LOCUS       DADOZM010000001        77560 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.001, whole genome shotgun sequence.
ACCESSION   DADOZM010000001 DADOZM010000000
VERSION     DADOZM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 77560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.001"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            27..545
                     /locus_tag="J8I47_000001"
     CDS             27..545
                     /locus_tag="J8I47_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ6321472.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            673..1356
                     /locus_tag="J8I47_000002"
     CDS             673..1356
                     /locus_tag="J8I47_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAZ6321473.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1665..1738
                     /locus_tag="J8I47_000003"
     tRNA            1665..1738
                     /locus_tag="J8I47_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Arg,seq:tct)
     gene            1897..2256
                     /locus_tag="J8I47_000004"
     CDS             1897..2256
                     /locus_tag="J8I47_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAZ6321474.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2379..4010
                     /locus_tag="J8I47_000005"
     CDS             2379..4010
                     /locus_tag="J8I47_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAZ6321475.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4072..4713)
                     /locus_tag="J8I47_000006"
     CDS             complement(4072..4713)
                     /locus_tag="J8I47_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAZ6321476.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4995..5303
                     /gene="rplU"
                     /locus_tag="J8I47_000007"
     CDS             4995..5303
                     /gene="rplU"
                     /locus_tag="J8I47_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAZ6321477.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5330..5674
                     /locus_tag="J8I47_000008"
     CDS             5330..5674
                     /locus_tag="J8I47_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAZ6321478.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5687..5980
                     /gene="rpmA"
                     /locus_tag="J8I47_000009"
     CDS             5687..5980
                     /gene="rpmA"
                     /locus_tag="J8I47_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAZ6321479.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6387..9221
                     /locus_tag="J8I47_000010"
     CDS             6387..9221
                     /locus_tag="J8I47_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6321480.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9412..9903
                     /locus_tag="J8I47_000011"
     CDS             9412..9903
                     /locus_tag="J8I47_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAZ6321481.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10006..10995
                     /gene="manX"
                     /locus_tag="J8I47_000012"
     CDS             10006..10995
                     /gene="manX"
                     /locus_tag="J8I47_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAZ6321482.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11028..11831
                     /locus_tag="J8I47_000013"
     CDS             11028..11831
                     /locus_tag="J8I47_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAZ6321483.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11852..12766
                     /locus_tag="J8I47_000014"
     CDS             11852..12766
                     /locus_tag="J8I47_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAZ6321484.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12849..13223
                     /locus_tag="J8I47_000015"
     CDS             12849..13223
                     /locus_tag="J8I47_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAZ6321485.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13362..13607
                     /locus_tag="J8I47_000016"
     CDS             13362..13607
                     /locus_tag="J8I47_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ6321486.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13779..14843
                     /locus_tag="J8I47_000017"
     CDS             13779..14843
                     /locus_tag="J8I47_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAZ6321487.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15089..15514
                     /locus_tag="J8I47_000018"
     CDS             15089..15514
                     /locus_tag="J8I47_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ6321488.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15507..15956
                     /gene="nusB"
                     /locus_tag="J8I47_000019"
     CDS             15507..15956
                     /gene="nusB"
                     /locus_tag="J8I47_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAZ6321489.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16053..16907
                     /locus_tag="J8I47_000020"
     CDS             16053..16907
                     /locus_tag="J8I47_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAZ6321490.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16908..18245
                     /locus_tag="J8I47_000021"
     CDS             16908..18245
                     /locus_tag="J8I47_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAZ6321491.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18250..18474
                     /locus_tag="J8I47_000022"
     CDS             18250..18474
                     /locus_tag="J8I47_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAZ6321492.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18476..19366
                     /locus_tag="J8I47_000023"
     CDS             18476..19366
                     /locus_tag="J8I47_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAZ6321493.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19375..20193
                     /locus_tag="J8I47_000024"
     CDS             19375..20193
                     /locus_tag="J8I47_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAZ6321494.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20300..20749
                     /locus_tag="J8I47_000025"
     CDS             20300..20749
                     /locus_tag="J8I47_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAZ6321495.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20767..22449
                     /gene="recN"
                     /locus_tag="J8I47_000026"
     CDS             20767..22449
                     /gene="recN"
                     /locus_tag="J8I47_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAZ6321496.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22504..22620)
                     /locus_tag="J8I47_000027"
     CDS             complement(22504..22620)
                     /locus_tag="J8I47_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAZ6321497.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22693..23649)
                     /locus_tag="J8I47_000028"
     CDS             complement(22693..23649)
                     /locus_tag="J8I47_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ6321498.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23820..24188
                     /locus_tag="J8I47_000029"
     CDS             23820..24188
                     /locus_tag="J8I47_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAZ6321499.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24358..24789
                     /gene="mraZ"
                     /locus_tag="J8I47_000030"
     CDS             24358..24789
                     /gene="mraZ"
                     /locus_tag="J8I47_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAZ6321500.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24810..25769
                     /gene="rsmH"
                     /locus_tag="J8I47_000031"
     CDS             24810..25769
                     /gene="rsmH"
                     /locus_tag="J8I47_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAZ6321501.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25774..26175
                     /gene="ftsL"
                     /locus_tag="J8I47_000032"
     CDS             25774..26175
                     /gene="ftsL"
                     /locus_tag="J8I47_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAZ6321502.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26176..28368
                     /locus_tag="J8I47_000033"
     CDS             26176..28368
                     /locus_tag="J8I47_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAZ6321503.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28394..29356
                     /locus_tag="J8I47_000034"
     CDS             28394..29356
                     /locus_tag="J8I47_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAZ6321504.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29370..30731
                     /locus_tag="J8I47_000035"
     CDS             29370..30731
                     /locus_tag="J8I47_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAZ6321505.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30757..31845
                     /gene="murG"
                     /locus_tag="J8I47_000036"
     CDS             30757..31845
                     /gene="murG"
                     /locus_tag="J8I47_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAZ6321506.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31860..33080
                     /locus_tag="J8I47_000037"
     CDS             31860..33080
                     /locus_tag="J8I47_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAZ6321507.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33236..34561
                     /gene="ftsA"
                     /locus_tag="J8I47_000038"
     CDS             33236..34561
                     /gene="ftsA"
                     /locus_tag="J8I47_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAZ6321508.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34584..35825
                     /gene="ftsZ"
                     /locus_tag="J8I47_000039"
     CDS             34584..35825
                     /gene="ftsZ"
                     /locus_tag="J8I47_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAZ6321509.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35842..36519
                     /locus_tag="J8I47_000040"
     CDS             35842..36519
                     /locus_tag="J8I47_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAZ6321510.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36533..37132
                     /locus_tag="J8I47_000041"
     CDS             36533..37132
                     /locus_tag="J8I47_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAZ6321511.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37147..37434
                     /locus_tag="J8I47_000042"
     CDS             37147..37434
                     /locus_tag="J8I47_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAZ6321512.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37483..38265
                     /locus_tag="J8I47_000043"
     CDS             37483..38265
                     /locus_tag="J8I47_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAZ6321513.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38286..38993
                     /locus_tag="J8I47_000044"
     CDS             38286..38993
                     /locus_tag="J8I47_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAZ6321514.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39255..42053
                     /gene="ileS"
                     /locus_tag="J8I47_000045"
     CDS             39255..42053
                     /gene="ileS"
                     /locus_tag="J8I47_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAZ6321515.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42102..43628)
                     /locus_tag="J8I47_000046"
     CDS             complement(42102..43628)
                     /locus_tag="J8I47_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAZ6321516.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43643..44290)
                     /locus_tag="J8I47_000047"
     CDS             complement(43643..44290)
                     /locus_tag="J8I47_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAZ6321517.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44474..44803)
                     /locus_tag="J8I47_000048"
     CDS             complement(44474..44803)
                     /locus_tag="J8I47_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAZ6321518.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44980..45708
                     /gene="sfsA"
                     /locus_tag="J8I47_000049"
     CDS             44980..45708
                     /gene="sfsA"
                     /locus_tag="J8I47_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAZ6321519.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45724..46737
                     /locus_tag="J8I47_000050"
     CDS             45724..46737
                     /locus_tag="J8I47_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6321520.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46737..48014
                     /locus_tag="J8I47_000051"
     CDS             46737..48014
                     /locus_tag="J8I47_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ6321521.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48077..50779
                     /locus_tag="J8I47_000052"
     CDS             48077..50779
                     /locus_tag="J8I47_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6321522.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50931..51248
                     /locus_tag="J8I47_000053"
     CDS             50931..51248
                     /locus_tag="J8I47_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6321523.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51278..51598
                     /locus_tag="J8I47_000054"
     CDS             51278..51598
                     /locus_tag="J8I47_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ6321524.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51706..53166
                     /locus_tag="J8I47_000055"
     CDS             51706..53166
                     /locus_tag="J8I47_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6321525.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53234..53455
                     /locus_tag="J8I47_000056"
     CDS             53234..53455
                     /locus_tag="J8I47_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321526.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53486..53668
                     /locus_tag="J8I47_000057"
     CDS             53486..53668
                     /locus_tag="J8I47_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAZ6321527.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53668..54081
                     /locus_tag="J8I47_000058"
     CDS             53668..54081
                     /locus_tag="J8I47_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAZ6321528.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54204..55385
                     /locus_tag="J8I47_000059"
     CDS             54204..55385
                     /locus_tag="J8I47_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6321529.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55774..55847
                     /locus_tag="J8I47_000060"
     tRNA            55774..55847
                     /locus_tag="J8I47_000060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55808..55810,aa:Arg,seq:cct)
     gene            complement(55884..56084)
                     /locus_tag="J8I47_000061"
     CDS             complement(55884..56084)
                     /locus_tag="J8I47_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6321530.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56389..57621)
                     /locus_tag="J8I47_000062"
     CDS             complement(56389..57621)
                     /locus_tag="J8I47_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAZ6321531.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57875..58444
                     /locus_tag="J8I47_000063"
     CDS             57875..58444
                     /locus_tag="J8I47_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6321532.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58622..59062)
                     /locus_tag="J8I47_000064"
     CDS             complement(58622..59062)
                     /locus_tag="J8I47_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAZ6321533.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59220..59984
                     /gene="map"
                     /locus_tag="J8I47_000065"
     CDS             59220..59984
                     /gene="map"
                     /locus_tag="J8I47_000065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAZ6321534.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60016..60939
                     /locus_tag="J8I47_000066"
     CDS             60016..60939
                     /locus_tag="J8I47_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAZ6321535.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61015..62154
                     /locus_tag="J8I47_000067"
     CDS             61015..62154
                     /locus_tag="J8I47_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAZ6321536.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62147..62947
                     /locus_tag="J8I47_000068"
     CDS             62147..62947
                     /locus_tag="J8I47_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ6321537.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62947..63774
                     /locus_tag="J8I47_000069"
     CDS             62947..63774
                     /locus_tag="J8I47_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ6321538.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63752..64486
                     /locus_tag="J8I47_000070"
     CDS             63752..64486
                     /locus_tag="J8I47_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ6321539.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64586..65452
                     /gene="rfbA"
                     /locus_tag="J8I47_000071"
     CDS             64586..65452
                     /gene="rfbA"
                     /locus_tag="J8I47_000071"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAZ6321540.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65466..66038
                     /gene="rfbC"
                     /locus_tag="J8I47_000072"
     CDS             65466..66038
                     /gene="rfbC"
                     /locus_tag="J8I47_000072"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAZ6321541.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66060..67088
                     /gene="rfbB"
                     /locus_tag="J8I47_000073"
     CDS             66060..67088
                     /gene="rfbB"
                     /locus_tag="J8I47_000073"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAZ6321542.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67186..68037
                     /gene="rfbD"
                     /locus_tag="J8I47_000074"
     CDS             67186..68037
                     /gene="rfbD"
                     /locus_tag="J8I47_000074"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAZ6321543.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68072..70105
                     /locus_tag="J8I47_000075"
     CDS             68072..70105
                     /locus_tag="J8I47_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321544.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70149..71429
                     /locus_tag="J8I47_000076"
     CDS             70149..71429
                     /locus_tag="J8I47_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAZ6321545.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71639..72445
                     /locus_tag="J8I47_000077"
     CDS             71639..72445
                     /locus_tag="J8I47_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321546.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72457..73677
                     /locus_tag="J8I47_000078"
     CDS             72457..73677
                     /locus_tag="J8I47_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321547.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73667..75253
                     /locus_tag="J8I47_000079"
     CDS             73667..75253
                     /locus_tag="J8I47_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6321548.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75292..77430
                     /locus_tag="J8I47_000080"
     CDS             75292..77430
                     /locus_tag="J8I47_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ6321549.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
BASE COUNT        25279 a        13130 c        16604 g        22547 t
ORIGIN      
        1 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
       61 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
      121 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
      181 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
      241 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
      301 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      361 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      421 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      481 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      541 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      601 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      661 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      721 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      781 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      841 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      901 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      961 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
     1021 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
     1081 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
     1141 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     1201 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1261 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1321 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1381 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1441 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1501 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     1561 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     1621 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     1681 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     1741 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     1801 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     1861 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     1921 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     1981 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     2041 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     2101 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     2161 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     2221 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     2281 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     2341 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     2401 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     2461 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     2521 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     2581 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
     2641 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
     2701 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
     2761 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
     2821 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
     2881 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
     2941 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
     3001 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
     3061 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
     3121 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
     3181 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
     3241 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
     3301 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
     3361 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
     3421 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
     3481 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
     3541 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
     3601 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
     3661 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
     3721 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
     3781 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
     3841 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
     3901 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
     3961 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
     4021 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
     4081 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
     4141 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
     4201 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
     4261 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
     4321 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
     4381 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
     4441 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
     4501 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
     4561 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
     4621 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
     4681 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
     4741 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
     4801 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
     4861 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
     4921 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
     4981 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
     5041 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
     5101 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
     5161 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
     5221 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
     5281 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
     5341 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
     5401 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
     5461 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
     5521 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
     5581 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
     5641 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
     5701 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
     5761 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
     5821 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
     5881 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
     5941 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
     6001 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
     6061 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
     6121 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
     6181 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
     6241 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
     6301 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
     6361 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
     6421 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
     6481 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
     6541 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
     6601 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
     6661 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
     6721 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
     6781 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
     6841 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
     6901 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
     6961 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
     7021 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
     7081 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
     7141 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
     7201 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
     7261 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
     7321 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
     7381 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
     7441 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
     7501 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
     7561 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
     7621 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
     7681 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
     7741 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
     7801 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
     7861 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
     7921 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
     7981 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
     8041 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
     8101 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
     8161 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
     8221 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
     8281 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
     8341 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
     8401 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
     8461 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
     8521 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
     8581 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
     8641 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
     8701 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
     8761 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
     8821 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
     8881 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
     8941 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
     9001 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
     9061 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
     9121 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
     9181 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
     9241 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
     9301 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
     9361 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
     9421 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
     9481 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
     9541 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
     9601 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
     9661 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
     9721 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
     9781 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
     9841 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
     9901 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
     9961 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
    10021 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
    10081 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
    10141 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    10201 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    10261 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    10321 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    10381 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    10441 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    10501 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    10561 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    10621 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    10681 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    10741 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    10801 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    10861 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    10921 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    10981 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    11041 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    11101 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    11161 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    11221 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    11281 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    11341 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    11401 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    11461 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    11521 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    11581 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    11641 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    11701 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    11761 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    11821 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    11881 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    11941 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    12001 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    12061 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    12121 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    12181 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    12241 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    12301 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    12361 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    12421 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    12481 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    12541 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    12601 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    12661 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    12721 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
    12781 gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga
    12841 gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt
    12901 ttacgggtct gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc
    12961 atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag
    13021 catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg
    13081 gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg
    13141 tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc
    13201 gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat
    13261 ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat
    13321 cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat
    13381 tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga
    13441 tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt
    13501 tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag
    13561 tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat
    13621 agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa
    13681 taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat
    13741 aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat
    13801 tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc
    13861 ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag
    13921 cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg
    13981 aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag
    14041 aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag
    14101 aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag
    14161 tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat
    14221 atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac
    14281 tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta
    14341 gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg
    14401 acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta
    14461 cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg
    14521 ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag
    14581 ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac
    14641 atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat
    14701 ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag
    14761 tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac
    14821 caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt
    14881 tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa
    14941 gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg
    15001 gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc
    15061 gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc
    15121 aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat
    15181 tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac
    15241 tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg
    15301 cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat
    15361 ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga
    15421 agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga
    15481 actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg
    15541 aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg
    15601 cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt
    15661 ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg
    15721 ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg
    15781 atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg
    15841 ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta
    15901 aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc
    15961 tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa
    16021 tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat
    16081 tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta
    16141 tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa
    16201 gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg
    16261 agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt
    16321 ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt
    16381 tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc
    16441 ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag
    16501 cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg
    16561 gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta
    16621 aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac
    16681 gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga
    16741 tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta
    16801 ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga
    16861 tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat
    16921 atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt
    16981 tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc
    17041 aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat
    17101 tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt
    17161 cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa
    17221 taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt
    17281 ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc
    17341 caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc
    17401 aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga
    17461 atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg
    17521 gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag
    17581 ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag
    17641 tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt
    17701 cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc
    17761 agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc
    17821 ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc
    17881 aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat
    17941 tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag
    18001 aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc
    18061 aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct
    18121 atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc
    18181 tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag
    18241 aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc
    18301 atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga
    18361 atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa
    18421 atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag
    18481 attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt
    18541 agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg
    18601 cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat
    18661 tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat
    18721 ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca
    18781 taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt
    18841 ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt
    18901 agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga
    18961 atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt
    19021 gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt
    19081 gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag atgatctact
    19141 cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga
    19201 aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga
    19261 gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg
    19321 cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa
    19381 aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc
    19441 aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg
    19501 ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt
    19561 tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga
    19621 aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat
    19681 ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga
    19741 caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac
    19801 tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg
    19861 atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa
    19921 ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag
    19981 acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg
    20041 ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac
    20101 ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt
    20161 tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa
    20221 gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat
    20281 gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt
    20341 gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat
    20401 atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc
    20461 ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac
    20521 taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt
    20581 tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta
    20641 caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac
    20701 tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg
    20761 aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt
    20821 tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt
    20881 atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga
    20941 gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa
    21001 ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg
    21061 tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg
    21121 agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc
    21181 cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag
    21241 aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa
    21301 gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa
    21361 gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa
    21421 aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg
    21481 aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc
    21541 gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg
    21601 ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt
    21661 cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc
    21721 gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct
    21781 gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta
    21841 cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa
    21901 aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt
    21961 ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac
    22021 attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa
    22081 ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt
    22141 attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat
    22201 aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt
    22261 gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa
    22321 acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct
    22381 ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa
    22441 aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta
    22501 tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat
    22561 tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat
    22621 tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac
    22681 aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat
    22741 aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt
    22801 aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga
    22861 agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt
    22921 catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac
    22981 caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc
    23041 tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt
    23101 ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc
    23161 caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg
    23221 ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg
    23281 gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt
    23341 gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt
    23401 tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa
    23461 ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat
    23521 ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca
    23581 gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata
    23641 tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga
    23701 atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa
    23761 cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca
    23821 tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg
    23881 gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg
    23941 atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa
    24001 tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt
    24061 tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt
    24121 tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt
    24181 acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat
    24241 ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta
    24301 gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg
    24361 ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa
    24421 cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc
    24481 ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct
    24541 aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt
    24601 gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa
    24661 ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa
    24721 gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc
    24781 ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca
    24841 tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta
    24901 cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc
    24961 tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt
    25021 atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct
    25081 tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc
    25141 agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca
    25201 tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat
    25261 tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa
    25321 tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta
    25381 aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc
    25441 aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg
    25501 caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag
    25561 acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac
    25621 ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc
    25681 ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg
    25741 cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat
    25801 tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct
    25861 tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct
    25921 ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca
    25981 gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa
    26041 atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt
    26101 actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta
    26161 aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat
    26221 ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga
    26281 ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt
    26341 acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa
    26401 agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca
    26461 attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa
    26521 aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat
    26581 gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc
    26641 tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa
    26701 ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt
    26761 tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa
    26821 ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat
    26881 caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc
    26941 gacggacaag atatctacac gactttagac agcagactac aaagttattt agaaacactg
    27001 atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca
    27061 aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa
    27121 ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca
    27181 ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag
    27241 aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac
    27301 tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg
    27361 atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga
    27421 tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta
    27481 aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa
    27541 atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc
    27601 agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg
    27661 caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa
    27721 agcgaaaact acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca
    27781 aaaaacggaa cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc
    27841 tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta
    27901 acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg
    27961 atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca
    28021 gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct
    28081 caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct
    28141 actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat
    28201 aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc
    28261 ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca
    28321 tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag
    28381 gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat
    28441 gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc
    28501 gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg
    28561 cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca
    28621 gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt
    28681 tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa
    28741 attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta
    28801 tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt
    28861 cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg
    28921 attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca
    28981 gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt
    29041 cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg
    29101 cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg
    29161 tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac
    29221 aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc
    29281 ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt
    29341 atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga
    29401 aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat
    29461 tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag
    29521 agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg
    29581 aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa
    29641 aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg
    29701 aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag
    29761 gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt
    29821 atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt
    29881 ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg attaccaata
    29941 tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga
    30001 accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac
    30061 tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt
    30121 tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg
    30181 aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt
    30241 ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc
    30301 atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg
    30361 attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta
    30421 aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt
    30481 cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg
    30541 ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc
    30601 ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca
    30661 gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc
    30721 gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga
    30781 ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa
    30841 ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct
    30901 gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg
    30961 cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc
    31021 agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg
    31081 tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt
    31141 gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg
    31201 gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta
    31261 gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca
    31321 gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt
    31381 ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat
    31441 gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc
    31501 aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact
    31561 tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt
    31621 acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg
    31681 attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt
    31741 gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct
    31801 tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag
    31861 tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga
    31921 ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc
    31981 caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg
    32041 ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga
    32101 tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt
    32161 taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg
    32221 gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg
    32281 cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat
    32341 taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa
    32401 aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt
    32461 tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag
    32521 tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta
    32581 tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt
    32641 tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata
    32701 aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc
    32761 ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga
    32821 tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat
    32881 cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa
    32941 gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat
    33001 ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt
    33061 caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac
    33121 cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa
    33181 cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc
    33241 aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc
    33301 tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat
    33361 taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg
    33421 acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa
    33481 catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat
    33541 caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga
    33601 acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa
    33661 agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc
    33721 taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga
    33781 aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt
    33841 tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa
    33901 gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc
    33961 tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc
    34021 atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga
    34081 accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat
    34141 tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt
    34201 cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt
    34261 cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac
    34321 aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa
    34381 aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac
    34441 aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa
    34501 agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta
    34561 attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg
    34621 cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg
    34681 aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa
    34741 attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg
    34801 gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag
    34861 ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag
    34921 gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg
    34981 tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg
    35041 ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag
    35101 ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac
    35161 aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg
    35221 ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta
    35281 gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa
    35341 catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat
    35401 tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata
    35461 tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg
    35521 caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac
    35581 aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt
    35641 cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta
    35701 gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg
    35761 aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa
    35821 aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt
    35881 caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca
    35941 aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca
    36001 gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata
    36061 aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt
    36121 gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag
    36181 aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt
    36241 tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt
    36301 gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt
    36361 ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt
    36421 agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca
    36481 tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct
    36541 tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata
    36601 ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca
    36661 gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac
    36721 ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact
    36781 acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca
    36841 acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc
    36901 tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt
    36961 agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga
    37021 tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat
    37081 tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga
    37141 actacgattt atactattgc actattactt catttacttc aacgggcagc ctatttctat
    37201 agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg
    37261 ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct
    37321 ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc
    37381 gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag
    37441 caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac
    37501 cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag
    37561 gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt
    37621 ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa
    37681 caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat
    37741 tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg
    37801 aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc
    37861 tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca
    37921 caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg
    37981 attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac
    38041 agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct
    38101 ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta
    38161 gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg
    38221 aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt
    38281 tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg
    38341 gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat
    38401 tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt
    38461 acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg
    38521 ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca
    38581 aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag
    38641 aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag
    38701 aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg
    38761 agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca
    38821 gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg
    38881 aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg
    38941 atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa
    39001 aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat
    39061 ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg
    39121 acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac
    39181 cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag
    39241 gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta
    39301 tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa
    39361 tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac
    39421 ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca
    39481 ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata
    39541 cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt
    39601 ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac
    39661 gagcagattt caaacgccta ggagttgcag gagattggga acatccttat gttacattag
    39721 atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata
    39781 tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag
    39841 ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag
    39901 acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga
    39961 cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag
    40021 ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg
    40081 gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg
    40141 caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag
    40201 acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg
    40261 gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag
    40321 aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat
    40381 taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt
    40441 ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata
    40501 aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta
    40561 aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg
    40621 catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag
    40681 aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa
    40741 aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat
    40801 ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg
    40861 ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc
    40921 aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac
    40981 catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta
    41041 aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc
    41101 ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct
    41161 taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga
    41221 atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag
    41281 acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg
    41341 acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat
    41401 cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc
    41461 aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga
    41521 ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt
    41581 acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg
    41641 atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa
    41701 gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac
    41761 aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac
    41821 cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg
    41881 atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag
    41941 ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa
    42001 tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa
    42061 aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca
    42121 cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt
    42181 tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt
    42241 tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa
    42301 tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct
    42361 gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca
    42421 aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata
    42481 tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt
    42541 ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc
    42601 aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg
    42661 aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga
    42721 tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt
    42781 tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta
    42841 cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt
    42901 aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga
    42961 tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc
    43021 cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct
    43081 tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc
    43141 tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga
    43201 ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa
    43261 aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct
    43321 aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat
    43381 tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg
    43441 attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg
    43501 attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac
    43561 aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca
    43621 ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa
    43681 agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga
    43741 tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca
    43801 attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca
    43861 atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag
    43921 gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca
    43981 catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa
    44041 ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc
    44101 ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt
    44161 tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat
    44221 tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat
    44281 ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa
    44341 aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca
    44401 acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga tatatatata
    44461 cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat
    44521 aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta
    44581 aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg
    44641 tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac
    44701 cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa
    44761 aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat
    44821 cactccttta tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc
    44881 gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa
    44941 ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa
    45001 atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga
    45061 gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc
    45121 gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt
    45181 gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca
    45241 attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta
    45301 tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc
    45361 ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct
    45421 tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca
    45481 caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca
    45541 acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc
    45601 ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag
    45661 gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa
    45721 ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca
    45781 gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca
    45841 caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt
    45901 ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt
    45961 caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg
    46021 acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt
    46081 gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta
    46141 aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa
    46201 gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg
    46261 gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc
    46321 cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt
    46381 tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca
    46441 gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca
    46501 caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat
    46561 ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct
    46621 tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat
    46681 gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga
    46741 acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta
    46801 tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag
    46861 tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa
    46921 acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta
    46981 ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt
    47041 tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct
    47101 tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc
    47161 aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa
    47221 tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga
    47281 cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg
    47341 acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga
    47401 tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga
    47461 ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg
    47521 taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac
    47581 aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag
    47641 atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt
    47701 tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac
    47761 aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag
    47821 agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc
    47881 ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata
    47941 acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag
    48001 caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg
    48061 ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa
    48121 ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc
    48181 aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc
    48241 agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc
    48301 tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca
    48361 gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga
    48421 aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga
    48481 gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc
    48541 gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca
    48601 agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca
    48661 ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg
    48721 tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac
    48781 tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt
    48841 gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa
    48901 acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt
    48961 catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg
    49021 tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga
    49081 agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct
    49141 cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt
    49201 tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt
    49261 cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga
    49321 tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga
    49381 tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg
    49441 gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac
    49501 attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa
    49561 aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga
    49621 acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct
    49681 aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag
    49741 acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa
    49801 agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata
    49861 tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc
    49921 attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac
    49981 gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt
    50041 agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa
    50101 agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg
    50161 tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt
    50221 agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt
    50281 attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat
    50341 cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat
    50401 agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga
    50461 tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac
    50521 cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt
    50581 catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt
    50641 ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg
    50701 aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga
    50761 aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc
    50821 gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc
    50881 atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa
    50941 aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc
    51001 aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg
    51061 cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt
    51121 tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca
    51181 atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa
    51241 tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga
    51301 agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat
    51361 tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga
    51421 atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa
    51481 tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc
    51541 gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat
    51601 acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata
    51661 aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca
    51721 agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta
    51781 gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct
    51841 gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac
    51901 agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt
    51961 tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat
    52021 aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc
    52081 ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct
    52141 tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca
    52201 ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt
    52261 acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac
    52321 attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt
    52381 ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta
    52441 gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga
    52501 ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc
    52561 tttggtattc atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca
    52621 caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc
    52681 gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct
    52741 tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc
    52801 tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat
    52861 atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca
    52921 ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta tcttggccta
    52981 gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta
    53041 gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc
    53101 ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa
    53161 caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg
    53221 aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt
    53281 gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa
    53341 gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact
    53401 aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat
    53461 ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg
    53521 ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat
    53581 ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag
    53641 agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca
    53701 tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc
    53761 aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat
    53821 aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca
    53881 tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc
    53941 tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat
    54001 atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat
    54061 atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga
    54121 agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct
    54181 tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga
    54241 ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc
    54301 gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg
    54361 atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt
    54421 acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga
    54481 tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt
    54541 atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata
    54601 aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa
    54661 taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc
    54721 cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc
    54781 aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata
    54841 tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg
    54901 atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg
    54961 aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt
    55021 ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa
    55081 attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg
    55141 cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa
    55201 aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat
    55261 tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag
    55321 aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt
    55381 tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc
    55441 atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc
    55501 ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg
    55561 atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat
    55621 tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta
    55681 gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa
    55741 aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca
    55801 atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc
    55861 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
    55921 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
    55981 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
    56041 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
    56101 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
    56161 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
    56221 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
    56281 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
    56341 ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc
    56401 gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt
    56461 gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa
    56521 ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc
    56581 taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga
    56641 aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt
    56701 atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc
    56761 atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt
    56821 acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg
    56881 catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg
    56941 gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa
    57001 ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc
    57061 taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc
    57121 aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc
    57181 agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc
    57241 tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc
    57301 gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg
    57361 atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc
    57421 tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc
    57481 ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt
    57541 gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag
    57601 ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt
    57661 agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta
    57721 tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa
    57781 aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg
    57841 aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag
    57901 agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac
    57961 tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa
    58021 aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga
    58081 agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta attatatcca
    58141 ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg
    58201 aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc
    58261 ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc
    58321 tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga
    58381 aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga
    58441 ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga
    58501 aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg
    58561 caatgaataa aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat
    58621 attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt
    58681 cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag
    58741 cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc
    58801 agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga
    58861 tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct
    58921 cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat
    58981 tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat
    59041 atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga
    59101 attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt
    59161 tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata
    59221 tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag
    59281 cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg
    59341 aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg
    59401 aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc
    59461 gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag
    59521 gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc
    59581 gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga
    59641 atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga
    59701 tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc
    59761 ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg
    59821 aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt
    59881 atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg
    59941 gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag
    60001 gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc
    60061 tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc
    60121 gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta
    60181 ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag
    60241 accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc
    60301 ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg
    60361 caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt
    60421 ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt
    60481 cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac
    60541 tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc
    60601 atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg
    60661 ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct
    60721 aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg
    60781 ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt
    60841 caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc
    60901 aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa
    60961 atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt
    61021 gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca
    61081 cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga
    61141 ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata
    61201 tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt
    61261 gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa
    61321 caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc agatatacgt
    61381 attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc
    61441 gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat
    61501 gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt
    61561 ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt
    61621 atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct
    61681 ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt
    61741 gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct
    61801 atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat
    61861 caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg
    61921 gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc
    61981 gtattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc
    62041 aatgagaaac atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt
    62101 acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt
    62161 aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact
    62221 agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa
    62281 caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga
    62341 tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc
    62401 tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt
    62461 tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat
    62521 tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta
    62581 cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt
    62641 gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag
    62701 ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt
    62761 catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt
    62821 gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa
    62881 actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt
    62941 tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa
    63001 tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt
    63061 tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat
    63121 catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca
    63181 gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat
    63241 tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt
    63301 ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct
    63361 caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt
    63421 gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat
    63481 tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt
    63541 cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga
    63601 taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta
    63661 tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac
    63721 cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt
    63781 gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag
    63841 tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga
    63901 tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc
    63961 ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga
    64021 tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac
    64081 tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga
    64141 cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg
    64201 gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt
    64261 aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt
    64321 gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga
    64381 tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt
    64441 tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa
    64501 atccttaata tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg
    64561 aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac
    64621 acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc
    64681 aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat
    64741 ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg
    64801 actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat
    64861 tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta
    64921 tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt
    64981 atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat
    65041 gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg
    65101 cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg
    65161 tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt
    65221 agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga
    65281 agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga
    65341 agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc
    65401 attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct
    65461 tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct
    65521 ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg
    65581 gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc
    65641 gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg
    65701 gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg
    65761 acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc
    65821 atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata
    65881 atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa
    65941 ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat
    66001 ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta
    66061 tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg
    66121 tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa
    66181 acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg
    66241 cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg
    66301 cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca
    66361 ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat
    66421 ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag
    66481 gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta
    66541 caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa
    66601 ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc
    66661 gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg
    66721 ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag
    66781 gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga
    66841 tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc
    66901 gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt
    66961 ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag
    67021 aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg
    67081 gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct
    67141 cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact
    67201 ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca
    67261 tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat
    67321 attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa
    67381 gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta
    67441 gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc
    67501 gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa
    67561 tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat
    67621 atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa
    67681 cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact
    67741 tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt
    67801 atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg
    67861 aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca
    67921 cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca
    67981 acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt
    68041 cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg
    68101 aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa
    68161 actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg
    68221 aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga
    68281 tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt
    68341 ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg
    68401 acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc
    68461 attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat
    68521 gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa
    68581 aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg
    68641 gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta
    68701 tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt
    68761 tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta
    68821 cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga
    68881 tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca
    68941 tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa
    69001 acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg
    69061 gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca
    69121 cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga
    69181 acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac
    69241 cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag
    69301 agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat
    69361 ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg
    69421 gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac
    69481 catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg
    69541 cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt
    69601 cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga
    69661 tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac
    69721 attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa
    69781 aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt
    69841 tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt
    69901 gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac
    69961 tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg
    70021 ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca
    70081 ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag
    70141 aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg
    70201 gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc
    70261 cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt
    70321 tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc
    70381 ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag
    70441 acttactgtt tcctttggtt attttatttt gtttagtaat taaatggatc gagtggacaa
    70501 aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc
    70561 gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc
    70621 tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat
    70681 tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag
    70741 cagtcgttgc agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt
    70801 tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc
    70861 catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg
    70921 tctctgaaaa agtcaatttg tttaccgatc gagaagatat ttggcataaa ttctatcaga
    70981 cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag
    71041 gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga
    71101 taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag
    71161 cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt
    71221 catttatgga ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg
    71281 ctatggccgt tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag
    71341 aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc
    71401 attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa
    71461 ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt
    71521 tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa
    71581 aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat
    71641 gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat tattgctaca
    71701 gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc
    71761 gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa
    71821 agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc
    71881 ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta
    71941 tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa
    72001 tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat
    72061 atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt
    72121 gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt
    72181 aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca
    72241 gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg
    72301 ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta
    72361 tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa
    72421 accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta
    72481 aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc
    72541 ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta
    72601 gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca
    72661 atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga
    72721 aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa
    72781 aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc
    72841 gatgatatca ttgaatttgc tgacatcgga gaccatattt atcaacctgt aaaaacttat
    72901 tcaagcggga tgttcgttcg tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt
    72961 ttgattgtgg atgaagcatt ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa
    73021 aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatatcagt
    73081 tcgattcgtc gtttttgtga tcgttcctat tggttgaaaa acggagaagt cgtcgaatat
    73141 ggcgatacga tggaagtaac gacgaattat gaaaactttt taaagaaaaa atcagttaaa
    73201 acagtggatc ggaataaaaa ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg
    73261 acgaaagcgg aattgttgga taaagacaaa aatccggtgg atatgctgga acaaggagaa
    73321 aaactatacg ttaaagtaac ttatgaagta aaagatgata caatcaaaaa tccggtttta
    73381 ggaattgcgc tacgaacagt cgacaatttt tatgtatgcg gacttaatac tctactagat
    73441 ggtatcaaga ttccttggaa aaaaggagag aatatctttt atctggagta tgaaaagtta
    73501 ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt
    73561 cctttggtat ataagacaaa gtacatgaac ttgtttgtca gcggttctta tatcggagaa
    73621 ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt
    73681 tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg
    73741 aagtaaagtt ttagagtttg gtcccggaaa cggccgatta acgaaacatt taatcggagc
    73801 aaaacaatgt gaagtgagca ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga
    73861 atttgctcaa gatggatttt atggtgatat cgaaagtttt gagtgggcaa actattatgc
    73921 tggacagaca tttgattacg tcttgtttgc ggatgtttta gaacatttgg tagatcctgg
    73981 aaaaacattg aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt
    74041 ccctaacttg gcgcataatt ctgtcatgat cgatttgttc aataaccaat tgccgtgggc
    74101 ttcttatggt ctcctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa
    74161 agtttttgaa aaagcgggat tattcattaa tattgaagat tatttgtact tagctgtagg
    74221 tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgct atgatttcaa
    74281 aatgcgacca tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagc
    74341 acaaagttcc attgcagagc cacaaaattc taattatgta aaagtgttag aagtaaccca
    74401 gcaaacgaaa caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa
    74461 tgagacacta agtttccccg tatcagaaac aacagaaaga atggtgtttc gcttcgcgca
    74521 acaacctagc tttatcgaat tcagcgcaga agctgccgga gaaaaactaa catttatcga
    74581 ttcgaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaattacc
    74641 agagttcgtt ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt
    74701 tatcggggaa ttgacaccaa caatgaaaga attgttggaa acgattcgtc ccatggctga
    74761 agtcacaaag cagctttctg agaaaaatga cgaattagaa agagaaaatc atcatcttct
    74821 tgaggaaaat acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt
    74881 gttggaaagt gatgaatgga caataaaaca tagactagga cgacgtaaga aagaaacttc
    74941 gaaaaaaatc caagaaaaag aactttctat atgtattgac gagaaaatct gggatgcaga
    75001 aacaaaaata ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agcccttgtc
    75061 ctataagtta tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagaaga
    75121 agtcaatcaa gcagaacagc tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct
    75181 ttgcgagcaa gaacgttcat tcttagtgga agcagtagct caaaatggcc aatcatggat
    75241 cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa
    75301 aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt
    75361 attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa
    75421 gaaaatgtga ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat
    75481 cttgatgtta agacccaaag cggtttcaag gtacgtttat cagggaaaat gagaggaaaa
    75541 gccatcttag acttccaaac agcgagacat caaaatggca tagctgtgaa attaaacggt
    75601 aaataccctt atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa
    75661 aaagggataa attatgcaag gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg
    75721 gaattgaagc caggatcgat cgactatgca gagtggatca gtcgtcatga gaaaccagat
    75781 ttgaaagagc aaaggaaaga agcacaagga tttgcgtacc gtccattgat ttccatactt
    75841 atgcctgtct ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtactggat
    75901 caaacgtatg atcattggga actatgtatc agtgatgatg cttctactga tccagctatc
    75961 cgaaagtgtc tggaatccta tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa
    76021 gaaaatggac acatcagtct ggcaactaat tctgcattgg aaatagcaga aggagaattt
    76081 atagcactct tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa
    76141 gtgctaaacg tacatccaga actggatgtg atctacagtg acgaagataa aatcgatgca
    76201 gatggcaatc gctttgatcc gcatttcaaa gcagactggt cgcctgatac attgatggga
    76261 aataattata tctctcactt aggcgtatat cgaacaagca ttgttaaaga acttggcggt
    76321 ttccgtaaag gctatgaagg ttctcaagat tatgatttgg tccttcgtgt gacagaacaa
    76381 atccctgcag atcatattta ccacattgac cgagtcttgt atcattggcg gacgatccca
    76441 ggatcaactg caagtaatgg cgaagccaaa agttatatct atgattccgg agtgaaagca
    76501 ttgacagatg ccctgtctag aagaaacatc aaaggaagcg tacgtcctgg cagaatttct
    76561 ggattttatg agattgctta tgacgtcttg caagaggatt tggtcagtgt gatcattcca
    76621 acgaagaatg gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct
    76681 tattcaaatt atgaaatcat tattgcggat aacggaagta cagatccaaa aatgcaagaa
    76741 ttatttgcag aatacaagca tcagctgaaa gaccgcttta tcgtagaatt gattgacatt
    76801 ccattcaatt attctcgaat taacaattta gcagcagaaa aagcaaacgg caaatacttt
    76861 ttattcttga ataacgatac agaagtgatt gagccagatt ggatgacggt gatggtttct
    76921 tatgcacagt ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca
    76981 acgcagcatg caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac
    77041 tatgatcgaa cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca
    77101 gtcactgcag cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat
    77161 gaaacattag aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt
    77221 cgttacaatg tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt
    77281 tatgaagata ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat
    77341 aaatggccag catatattgc gcatgatcca ttttacaatg ataatttaac aaaagacgga
    77401 atcggcgact tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt
    77461 cgctcgcaca taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc
    77521 aaaaaaataa gtcgaggttg ggacagaagt gtttaactcc
//
LOCUS       DADOZM010000002        70071 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.002, whole genome shotgun sequence.
ACCESSION   DADOZM010000002 DADOZM010000000
VERSION     DADOZM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 70071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.002"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="J8I47_000081"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="J8I47_000081"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAZ6321550.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="J8I47_000082"
     CDS             1007..1927
                     /locus_tag="J8I47_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6321551.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="J8I47_000083"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="J8I47_000083"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAZ6321552.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="J8I47_000084"
     CDS             3233..4711
                     /locus_tag="J8I47_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ6321553.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="J8I47_000085"
     CDS             4988..7048
                     /locus_tag="J8I47_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321554.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="J8I47_000086"
     CDS             7131..7700
                     /locus_tag="J8I47_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6321555.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="J8I47_000087"
     CDS             complement(7875..8681)
                     /locus_tag="J8I47_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321556.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="J8I47_000088"
     CDS             8819..10333
                     /locus_tag="J8I47_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAZ6321557.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            10894..11778
                     /locus_tag="J8I47_000089"
     CDS             10894..11778
                     /locus_tag="J8I47_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6321558.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            11987..12565
                     /gene="pgsA"
                     /locus_tag="J8I47_000090"
     CDS             11987..12565
                     /gene="pgsA"
                     /locus_tag="J8I47_000090"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAZ6321559.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            12805..14046
                     /locus_tag="J8I47_000091"
     CDS             12805..14046
                     /locus_tag="J8I47_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAZ6321560.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            14155..15204
                     /gene="recA"
                     /locus_tag="J8I47_000092"
     CDS             14155..15204
                     /gene="recA"
                     /locus_tag="J8I47_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAZ6321561.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            15615..17171
                     /gene="rny"
                     /locus_tag="J8I47_000093"
     CDS             15615..17171
                     /gene="rny"
                     /locus_tag="J8I47_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAZ6321562.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(17250..18056)
                     /gene="proC"
                     /locus_tag="J8I47_000094"
     CDS             complement(17250..18056)
                     /gene="proC"
                     /locus_tag="J8I47_000094"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAZ6321563.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            18197..20770
                     /gene="mprF"
                     /locus_tag="J8I47_000095"
     CDS             18197..20770
                     /gene="mprF"
                     /locus_tag="J8I47_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAZ6321564.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            20924..21775
                     /locus_tag="J8I47_000096"
     CDS             20924..21775
                     /locus_tag="J8I47_000096"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAZ6321565.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            22020..22958
                     /locus_tag="J8I47_000097"
     CDS             22020..22958
                     /locus_tag="J8I47_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAZ6321566.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            22981..23661
                     /locus_tag="J8I47_000098"
     CDS             22981..23661
                     /locus_tag="J8I47_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321567.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            23634..24458
                     /locus_tag="J8I47_000099"
     CDS             23634..24458
                     /locus_tag="J8I47_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ6321568.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            24625..25443
                     /gene="purR"
                     /locus_tag="J8I47_000100"
     CDS             24625..25443
                     /gene="purR"
                     /locus_tag="J8I47_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAZ6321569.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            25946..27319
                     /gene="glmU"
                     /locus_tag="J8I47_000101"
     CDS             25946..27319
                     /gene="glmU"
                     /locus_tag="J8I47_000101"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAZ6321570.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            27413..28384
                     /locus_tag="J8I47_000102"
     CDS             27413..28384
                     /locus_tag="J8I47_000102"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAZ6321571.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      28390..28492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            28558..29127
                     /gene="thiT"
                     /locus_tag="J8I47_000103"
     CDS             28558..29127
                     /gene="thiT"
                     /locus_tag="J8I47_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAZ6321572.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(29208..29981)
                     /locus_tag="J8I47_000104"
     CDS             complement(29208..29981)
                     /locus_tag="J8I47_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321573.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            30248..31948
                     /locus_tag="J8I47_000105"
     CDS             30248..31948
                     /locus_tag="J8I47_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAZ6321574.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            31918..32136
                     /locus_tag="J8I47_000106"
     CDS             31918..32136
                     /locus_tag="J8I47_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321575.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            32156..33046
                     /locus_tag="J8I47_000107"
     CDS             32156..33046
                     /locus_tag="J8I47_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAZ6321576.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            33064..33348
                     /locus_tag="J8I47_000108"
     CDS             33064..33348
                     /locus_tag="J8I47_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321577.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            33835..34449
                     /gene="gmk"
                     /locus_tag="J8I47_000109"
     CDS             33835..34449
                     /gene="gmk"
                     /locus_tag="J8I47_000109"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAZ6321578.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            34454..34759
                     /locus_tag="J8I47_000110"
     CDS             34454..34759
                     /locus_tag="J8I47_000110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAZ6321579.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            35012..37420
                     /gene="priA"
                     /locus_tag="J8I47_000111"
     CDS             35012..37420
                     /gene="priA"
                     /locus_tag="J8I47_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAZ6321580.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            37435..37926
                     /gene="def"
                     /locus_tag="J8I47_000112"
     CDS             37435..37926
                     /gene="def"
                     /locus_tag="J8I47_000112"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAZ6321581.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            37919..38857
                     /locus_tag="J8I47_000113"
     CDS             37919..38857
                     /locus_tag="J8I47_000113"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAZ6321582.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            38857..40215
                     /gene="rsmB"
                     /locus_tag="J8I47_000114"
     CDS             38857..40215
                     /gene="rsmB"
                     /locus_tag="J8I47_000114"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAZ6321583.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            40228..40968
                     /locus_tag="J8I47_000115"
     CDS             40228..40968
                     /locus_tag="J8I47_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAZ6321584.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            40965..43034
                     /gene="pknB"
                     /locus_tag="J8I47_000116"
     CDS             40965..43034
                     /gene="pknB"
                     /locus_tag="J8I47_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAZ6321585.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            43199..44098
                     /gene="rsgA"
                     /locus_tag="J8I47_000117"
     CDS             43199..44098
                     /gene="rsgA"
                     /locus_tag="J8I47_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAZ6321586.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            44111..44761
                     /locus_tag="J8I47_000118"
     CDS             44111..44761
                     /locus_tag="J8I47_000118"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAZ6321587.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            44762..45400
                     /locus_tag="J8I47_000119"
     CDS             44762..45400
                     /locus_tag="J8I47_000119"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAZ6321588.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            45555..46409
                     /gene="mreC"
                     /locus_tag="J8I47_000120"
     CDS             45555..46409
                     /gene="mreC"
                     /locus_tag="J8I47_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAZ6321589.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            46413..46922
                     /gene="mreD"
                     /locus_tag="J8I47_000121"
     CDS             46413..46922
                     /gene="mreD"
                     /locus_tag="J8I47_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAZ6321590.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            47177..48751
                     /locus_tag="J8I47_000122"
     CDS             47177..48751
                     /locus_tag="J8I47_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321591.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(48819..50618)
                     /locus_tag="J8I47_000123"
     CDS             complement(48819..50618)
                     /locus_tag="J8I47_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAZ6321592.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            50741..51247
                     /locus_tag="J8I47_000124"
     CDS             50741..51247
                     /locus_tag="J8I47_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAZ6321593.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            51326..52048
                     /locus_tag="J8I47_000125"
     CDS             51326..52048
                     /locus_tag="J8I47_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ6321594.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(52126..53526)
                     /locus_tag="J8I47_000126"
     CDS             complement(52126..53526)
                     /locus_tag="J8I47_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAZ6321595.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(53696..54670)
                     /locus_tag="J8I47_000127"
     CDS             complement(53696..54670)
                     /locus_tag="J8I47_000127"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAZ6321596.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            55023..55583
                     /locus_tag="J8I47_000128"
     CDS             55023..55583
                     /locus_tag="J8I47_000128"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAZ6321597.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            55600..59121
                     /gene="mfd"
                     /locus_tag="J8I47_000129"
     CDS             55600..59121
                     /gene="mfd"
                     /locus_tag="J8I47_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAZ6321598.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            59136..60731
                     /locus_tag="J8I47_000130"
     CDS             59136..60731
                     /locus_tag="J8I47_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ6321599.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            60742..61011
                     /locus_tag="J8I47_000131"
     CDS             60742..61011
                     /locus_tag="J8I47_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAZ6321600.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            61078..61503
                     /locus_tag="J8I47_000132"
     CDS             61078..61503
                     /locus_tag="J8I47_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAZ6321601.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            61549..62019
                     /locus_tag="J8I47_000133"
     CDS             61549..62019
                     /locus_tag="J8I47_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAZ6321602.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            62139..63584
                     /gene="tilS"
                     /locus_tag="J8I47_000134"
     CDS             62139..63584
                     /gene="tilS"
                     /locus_tag="J8I47_000134"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAZ6321603.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            63584..64129
                     /gene="hpt"
                     /locus_tag="J8I47_000135"
     CDS             63584..64129
                     /gene="hpt"
                     /locus_tag="J8I47_000135"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAZ6321604.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            64219..66330
                     /gene="hflB"
                     /locus_tag="J8I47_000136"
     CDS             64219..66330
                     /gene="hflB"
                     /locus_tag="J8I47_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAZ6321605.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLSEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            66547..67434
                     /gene="hslO"
                     /locus_tag="J8I47_000137"
     CDS             66547..67434
                     /gene="hslO"
                     /locus_tag="J8I47_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAZ6321606.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            67435..68439
                     /gene="dusB"
                     /locus_tag="J8I47_000138"
     CDS             67435..68439
                     /gene="dusB"
                     /locus_tag="J8I47_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAZ6321607.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            68550..70046
                     /gene="lysS"
                     /locus_tag="J8I47_000139"
     CDS             68550..70046
                     /gene="lysS"
                     /locus_tag="J8I47_000139"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAZ6321608.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT        23179 a        12438 c        15068 g        19386 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagaggctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacgagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg
    10441 catcaagtaa taagaacagc caagtaaaaa cagcttctta atgtttttaa cttggttgga
    10501 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    10561 ttttgacctg agtattagag aaacatacgg aaaaatatct ccttatattt tgccggtttt
    10621 tgagctaata ccgaggaaca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgc
    10681 cgtttaactc cgagtcacaa ggaacaagtt caaaaaacag cttcttatat ttattggata
    10741 ttgtgacttg tgaccgagga gttggcagga gaacaatgtt tattcggaaa aaaggagctg
    10801 tgacatgact tttgtcacag cacccaaaca taacgtcttt cgttgcaaca gcagatgctt
    10861 cggagaatca aacagactgg agaagatgaa cgagtggctt ttgaaaccat cggagaaaaa
    10921 ttacggcaag cacgcctgaa taaaaaaata tctttagatg aattacagca gattacaaaa
    10981 attcaaaaac gttatttgga agcaatcgac aatgatgatt ttgaccaatt gcctggtaaa
    11041 ttttacgtgc gggcatttat tcggcaatac gctgaagctg taggagaaga tggagaccat
    11101 ttagtcgatg tttttgatgg gaaaagcaaa ttgacaggat cggtccctga acgtccgcag
    11161 cctgaaacag taagtacttc tagaaaagca atgcatcagg aagaatcgca tccaagtcgc
    11221 ttttggacaa gtcttcctgt cattttatta ggattggttg ctttggcgat tgtcgttgtc
    11281 gtgggctata tgacttggca agatcgtcaa gctgatccaa taatcggaga cacagcttct
    11341 tcgcttaaag tagaaggatc agtcgaggag tcctcttcct cagagcaaag cacgcagacc
    11401 aaagagtctt ctacagtaga atcaacaaca aaatcaagtg aagaaaagaa aatggcaata
    11461 gctacaggag aagattctgg cagtgccatc gcagtaaata tcacagacgc taccaagcct
    11521 gtaacccttg aatttactgc tgtcaatcgt gtatggttag gtgtgctagt agacaatgcc
    11581 tatgtttatc aagggacact ggcagcaaat gaaacacaga gtaccgtgtt accggaaaca
    11641 gcaacaaatg cgaccatcac gattggtgca gcaagcaatg caacgatcaa agcgaatgga
    11701 gaatccgtac cagttaatcc aggggaaaac aatcagagtc caaaaaatgt gaaccttact
    11761 cttcaatacg cagaatgatc gtataaatta gtttacgcat ctgtttactt gtaggtataa
    11821 aaaagaagga tcgtgacaaa tctgtgtcgc ggtccttctt cgttaattca aaaagatcaa
    11881 tttcaataaa aatactcctt tgaataaatt catttggaaa agtaaataat atcggtggaa
    11941 acttcgctga tagtcacgta gaatagggaa gaaaggagtc gatagtgtga atttaccaaa
    12001 taaattaact gtacttagaa tttgtatgat cccgattttc attttggttg taggtttacc
    12061 tttagattgg ggacaattta acgtaggaga caccacactg gctgtcacac agctagtagg
    12121 tgcagttatt tttgctttag ctagtatcac agactggtta gatggaaaaa ttgcccgtgc
    12181 gcgaggcctt gtcactaatt ttggcaagtt tgcggacccg ttggcagata aaatgctagt
    12241 tatgactgct tttatcatgt tggttgacca aggaaaggcc cctggttggg tagtagcgat
    12301 cattgtctgt cgtgaattag ccgtcacagg cttgcgttta ctacttgtcg aacatggaga
    12361 agtcatggct gcagcttggc cgggaaaagt caagacggct acacaaatgg ttgctattat
    12421 cttgctgttt atcaataata tcccattcag cctgatcggt attccagtag atcaaatcat
    12481 gctttatgct tgtctgatct ttactatcta ttcaggtgtg gattacttca tcaaaaacag
    12541 cggtgtgttc aaaggatcaa tgtaagtatc gtttagtgga gaggttgtga gcctgccgtc
    12601 taactctgag tcacaaggga taatttcaaa aaacagcttt tcatgtttta tgtatattgt
    12661 gacttatgac cgaggttttg gcaggagaac actgtttatt tggaacaagg agctgtgaca
    12721 agatttttgt cacagctcct tgccctcttt ttaaataaaa aaatataaaa atacagcgta
    12781 tactatataa gggagcggga agtaatgaaa gcagaaataa tagctgtcgg aacagaactt
    12841 ttactaggac aagtcgtgaa tacgaatgcc actttcttgt cagaacagct agctgatcta
    12901 ggtatcgaag tttattatca aacagttgta ggagataatc agcagcgttt agaagagttg
    12961 attgctctag ctgaaacaag aagtgaactc atactcttat gtggcggatt aggaccgaca
    13021 gaagatgacc tgacaaaaga agcaactgcg gctcacctcg gtaagtctct catacaaaat
    13081 acggaaggat acaaaaaatt attggcatat tttgaaacaa cccatcgaaa aatgaccaaa
    13141 aacaatctgc aacagtccca gattatcgaa ggcggggttc ctctaccaaa tcgtacaggg
    13201 cttgctttag ggacttttta tcaaacagat acacatgcgt atatccttct tcccggtccg
    13261 cctaatgagt tgaaaccaat gtttgtggaa caagtacgtc ctttactaga ggaacgtttt
    13321 ccttctgaag aaaaactaat ttctaaagtt ttgcgctttt atgggattgg tgaatcgcga
    13381 ctggtcactg agctaaaaga tctgattgag acacagatca atccaacgat cgcaccatat
    13441 gccaaaccaa atgaagtgac attacgactg acagtgaaaa caaatgatgt tcaggcaggg
    13501 aatcaagctc ttttagcatt agaagaaaaa atccaagaac gagtagggga atatttttat
    13561 ggatacggcg atgataattc gctagcaaaa gtcgtagtgg aactactgaa agaaaacaag
    13621 cagactgtaa cagcagcgga aagtttgaca gccggcgcct tccaagccgc attaggcgac
    13681 attgctggtg tatctgaagt atttccaggc ggattcgtta cctactcgct ccaaacaaaa
    13741 gctggttttc tggagattga tccggagcta ttagcagaat atggaacagt cagcaaagaa
    13801 tgtgtggagc agatggccat tcaagcacgt gagatagcaa gaacagatta cagtgtagct
    13861 ttcaccggag tggctggacc ggacacgtta gaaggtcaat ctgcaggaac agtatggatc
    13921 gctatttcat ctaaagacgg cgtggtttcg gaaaaatatc actttgccag agatcgtgct
    13981 gctatcagat ataatgcggt tatgcaagga tttgatcttt tgagaaaaca gcttttaaga
    14041 aaatgataaa tgcgaatatt tgttcggttt tttcttgctt tttttcacta aaaaagatat
    14101 gataaggata atgaaatgtt gctctgattt gtaaatataa ggaggattat cgtattggca
    14161 gatgatcgta aagcagcctt agatgctgca ctgaaaaaaa ttgaaaagag ctatggtaaa
    14221 ggctctatta tgaaattggg cgaaaaaatc gaccaacaaa tctctacaat tccaagtggc
    14281 tctcttgcgt tagatgtcgc tttaggtgtc ggcggttacc cccgtggacg tatcatcgaa
    14341 gtatatggac ctgaaagttc aggtaaaaca acagttgcac tacacgctat tgcagaagta
    14401 caaaaaaatg gcggaacggc cgctttcatt gatgctgagc atgcgttaga tccgcaatat
    14461 gcacaaaaat taggtgtgaa tatcgatgaa ctacttcttt cacagcctga cacaggagaa
    14521 caaggtctag agatcgctga tgctttagta tcaagtgggg ctgtagatat cgtagtagtc
    14581 gattcagttg ctgctttagt tccacgagca gaaatcgacg gcgaaatggg tgactcacat
    14641 gtcgggttac aagcacgttt gatgtctcaa gcattgcgta aactctctgg ttcgatcaac
    14701 aaaacaaaaa caatcgctat tttcatcaac caaatccgtg aaaaagtagg cgtgatgttt
    14761 gggaatccag aaatcactcc tggaggacgc gcattgaaat tctacgcaac gatccgtttg
    14821 gaagtacgtc gtgcagaaca attgaaacaa ggtacagata ttgttggtaa ccgtacaaag
    14881 atcaaagtcg taaaaaacaa agtagcacct cctttcaaaa tcgctgaagt agatgtcatg
    14941 tatggtttag ggatctcaca agaaggagaa cttctagaca tggcagtaga aaaagacatt
    15001 gttgataaaa gcggtgcttg gtattcttat aaagaagatc ggattggtca aggacgggaa
    15061 aacgctaaaa tctatatggc taatcatcct gaaatgatgg cagaagtttc tgctctagtt
    15121 agagcagctt atggtattgg tgaagaagtc gctgtcccag aagatgaaaa aggacaagaa
    15181 gaattgcctt tagtagaaga ataaaaatta tgataaacaa aaacggctgg tctgaagagt
    15241 aggtactctc cttgacctgc cgttttttta ttgaataaag aaatcgtgag ttatttaatt
    15301 atacgagtgg gtacataaaa taaaaatatc tgttcatcta caaagaataa tttaatagaa
    15361 aagtttctca atttaaaggt ttacctatat gaaactagtg tcatttattt taaaaaccat
    15421 cgtttaatga ataagtagaa agccgtttct ttgtttattt aaagttgaca ctacggggta
    15481 caaacattag aataaagttg tgcatgtttt ttgtacacat atatgcgaag atgcatattg
    15541 acgagtttag aataatttat catagaagct gtatagatga taaaaatgaa aaagatagta
    15601 tggaggtgga ttttatggta ttcaacattc tcctcgctat catcggttta attgtcggtc
    15661 ttggtttagg gtttatggtc gcaaagtcac gtcacgagaa agaaatagct ggtgcacaat
    15721 catcagctgc tggaatcatc gatagtgcaa aaaaagaagc agaaactcta aaaaaagaag
    15781 cattgttaga agctaaggaa gaaaatcaga aataccgttc tgaagttgaa agtgagctga
    15841 gagaaagtaa gttagaatta aaatctcaag agaatcgttt gattcaacgc gaacaaacgc
    15901 tgaatcgtaa agatgactcg ttagaaaaac gcgagaactc acttgaaagt aaagaagaag
    15961 cacttagttc aaaacagcaa ttaattgaag agcgagaaaa agacgttgaa aaaatgattg
    16021 aagatcaaca aaaagaacta gaaaaaattg ctggtttatc aaaagaggac gcacgagatg
    16081 tcatcatgaa atcgactgaa gaagagctga atcatgagtt aactctaatg gtaaaagaat
    16141 ctgaacagcg tgccaaagaa gaagcagatc gcaaagcgaa aaacttatta tcattagcga
    16201 ttcaacgctg cgcagcagat caagtttcag aaacgactgt ttcagtcgtt acccttccga
    16261 atgatgaaat gaaaggtcgt atcattggac gagaaggtcg taatattcga acgcttgaga
    16321 cgctgactgg tatcgattta atcatcgatg atacaccgga agcagttgtt ctttccggat
    16381 tcgatccaat ccgaagagaa atcgcacgga tgaccttgga aaaactgata caagatggac
    16441 gaatccatcc agcgcgtatc gaagaaatgg tcgagaaatc tcgtaaagaa atggatgaac
    16501 gtattcgcga atacggggaa gaggcagcgt ttgaagtcgg tgctcataca cttcatccag
    16561 atctaatcaa gatcatcgga cgtctgcgat tcagaactag ttatggacaa aacgtcttga
    16621 agcattcggt cgaggttgca aaattagctg gtatcttagc agcagagctt ggcgaagata
    16681 tccaattagc aaaacgtgcc gggttgctcc atgatattgg gaaagcactt gaccatgaga
    16741 ttgaaggatc tcacgttgag atcggtgcgg aattagctgc caagtacaaa gaaaacccag
    16801 tagtaatcaa cgcgatcgct tctcatcatg gagatgtgga agctacttct gttatctctg
    16861 tccttgtagc tgcagcagat gcgctctcag cagcaagacc tggtgcgcga agtgaatcat
    16921 tagaaaacta catccgtcgt ttaagaagtc tggaaaatat ttcgaacagc ttcgccggtg
    16981 tagaatcaag ttacgcagtt caagctggac gcgaagttcg ggtaatggtc aagccagaag
    17041 aaatctcgga tttagacgct gttcgtttag tacgtgatat tcgtaaacga atcgaagaag
    17101 aactggatta tcctggacac atcaaagtaa tcgttattcg ggaaactcgt gcaactgatt
    17161 atgctaaata ataaaaaata caaaagaaga gccgcggcaa aagccacagc tcttcttttt
    17221 ttgtaaggca cgaaaaaaca ttgctatcat tattttttat tcaacatctc aagatctttc
    17281 tcgatcgtcg tattgattcc tttgatgatt gctgggatga atcctgcttc ttctaaggca
    17341 actacacctg caacagttgt accgccagga gaagaaactt ggtctacaag ttcccatgga
    17401 tttttatcac ttgaaaggaa tttttgacaa gaacccataa ttgcttttgc agcaatctca
    17461 gtggctgtct gtttggacaa accattcagt acaccagtcc gtcccatcgc atcgatcagc
    17521 atgtaaacga atgcgggtga gctgccagca aggccaatga atgtactgaa atctgattct
    17581 gctaaaggat agacgctgcc gattgcttca aaaatcatcg tagcttgctg aagctgttcc
    17641 tccgtggcag cttcattttt gcaaatcgca gaaacgcctg ctcctacgct gacattgaca
    17701 ttcggcatca cacgtacgat ggaggcctga ggatcttcaa gcgctgtttc taaatctatt
    17761 aatgattttc ctgaagcaat agaaatgaat aagactttct ggctatattc tcttagttct
    17821 tccaatactt tttcaatgat ttttggtttc acggctaaaa gaacgatatc tgattgtgat
    17881 gcaacttcct gattactttt gcatgcatgg acgccagttt cagcagcaaa tttttgtgca
    17941 ttttcaaaat gtccactgga aacgaacacc tcttctttcg agactgttcc tttttttaat
    18001 aaaccatcaa taatagcttg tgccatattc cctgtgccga taaatcctac tttcatgtta
    18061 tcctctcctt tagcattagg atatctttct ttttctatca tcgcaagcaa agaacaggtc
    18121 taaagtggaa aaattaaaat aaataagata acattggggt atgacacaaa agtatacaag
    18181 ttaggaatgg gaaaatgtga aaaataaacg atacttgatg ctgcaatgga taaaggaaca
    18241 tagtctgatt ttaaaactga tttttttcgg ctccgtgtta gtctttgtgg caaatcaagt
    18301 cgcaaatatt gctaatggca tgagttggca agatatttgg caaaggatgg gacaacaaag
    18361 ccgcatgacg atcctgctca tgattctggc tggactgctg ggtgtcactc cgatgttgtt
    18421 gtatgattgg gtgacgatca gagtcttaga aaaacaagga aaaccaaaaa tggaaagaaa
    18481 agagtttctt cttgctgcgt gggtaacaaa cacgatcaac aatctcgccg gttttggtgg
    18541 tgtggtagga gcaagtctgc gggctagttt ttatggaaag cagacaaata gaaaaatggt
    18601 tttagccact gtatcaaaag tcgcactatt tatgctgact ggtttgtctg tatggtcgtt
    18661 tcttacattt attgatgtct tttttattca gtcagaaagt atttttcgaa gctattggat
    18721 ctggcttttc ggcggtagtt ttttagcacc agtcattttc ttttttgctt atctaaagag
    18781 aaagacattg ttcttcgagt tttatccaaa aggcttgctt ggtctgctac tggcatcctt
    18841 tggacaatgg acgggagccc ttgcggtatt tttgatcatc ggcaagttga tgagaataga
    18901 tatttcgatg ctttcgatct atcctatgtt catgattgct acattgatag gaatgctgac
    18961 gatggtccca ggtggaatgg ggacttttga cgttttgatg atcttaggaa tgagtcaatt
    19021 gtctgttcgg caagatgtag cagtagtttg gctattatat tatcggttgt tttattatgt
    19081 tctacctttt atgacgggac ttctattgtt catcacacat acaggagtca aaatcaatcg
    19141 atttttagat aacattccgc gactgttttt ccaaaaggtt gcgcatatcg tgctcgtaag
    19201 tgctgtctat tttgcgggga tcatgatggt acttctatca acaatcacta atctgtctgc
    19261 aatcagtcgg ctatttcaag tattattgcc gttctcattc aattttttag atcagacagt
    19321 gaatctattg gttggctttt tattactcgg ccttgctaga gcactttcag taaaagtcaa
    19381 aaaggcattt ctgcctacga ttgctttact atcttttggt attttaaata caatcacacg
    19441 tacgttatct tggcagttga tattggtata tgtcttgatt ctgttagctg tgtggttatc
    19501 cagaaaagaa ttttatcgaa caaaattcgt ttattcttgg ggagctataa tctttgatgc
    19561 tagcttgttc ggctttttgt ttatcgttta cgcaattgca ggctatcaca gcggacgctt
    19621 tttagtcggt cgaataacag gaaatcattt tattttgttt ccttcagatg atgtgtggtt
    19681 ctcagggctt attggtttgg gcatctcatt gctagcttta gtggcgcttt atcaatattt
    19741 agctgaagat actacgaagc ttggcagtga gtacgaaaaa gaccgttttg accgtttatt
    19801 ggaaacatac ggaggcacag ctacaagcca tcagcttcgc ttgtcaggtt acagttattt
    19861 ttattaccaa gaagacgacc aagatcaagt cgtttttggt tatcagatca aaggaaacaa
    19921 atgctttgtt ttaggagatc cagttggcaa tcctaaaatg atgcgggcag caacgaatca
    19981 gtttatgaaa caagcagatt gtttaggcta tcagcttgcg ttttataaga tatcagaaag
    20041 ctacgtagtg atgcttcatg aattgggttt tcacttcacg aaagtaggtg aagcagggat
    20101 tgttgatcta gcaacagaag acttgtctcg gctgagtcag caacttgaat atcaacgttt
    20161 gatgaaagaa ggataccatt ttacttggta cgatcaactg ccggaagaat tacatcaagc
    20221 tgtaaatgaa gtttcacaaa aatggcttca aggtgcacga gaaaaatact tctctgtggg
    20281 acgatttgat aaatcatatg tattatctag cggtgtcggt attgtccgta aagatcaaaa
    20341 agtcgttggc ttcatcaccg ctcagccgat gacgaagaag caggcgggat atgatttgtt
    20401 gcgggtctta cctaaagaat ctgaggaatt agctgactat ctgttagcca atctgtttat
    20461 tgtatacgag aaaatgggct actcggaagc caatctaggc ttagctccat tagctaatgt
    20521 tggagaaaca gatttttctt tctttcaaga aaaagtcatg catatcgtat ataattatgg
    20581 taattttttc tattccttcc agtctgctta tgaaaagaaa ttaaaatatg tcgatcattg
    20641 ggaaggacgt tattttgctt ataggaaagg aagtaatttc ggatttgcta ccatgcagct
    20701 actcagtctg ataggaaaag aaaaaggaaa tgttccgagt ttggcagaag aagtgctcac
    20761 agaattataa gaatcactag aacttgtttg gattatgaag agaaaattct gtgaaagctg
    20821 ttgaaacgat gacaaaatca accactaggc agacataagc tatactttta tttttcccta
    20881 tgatagaata cttgcgactt agacttttcg aatgggtgag aggatggaat tgattgaaaa
    20941 agcgccagcc aagataaatt taggactgga tgtattatat agacggcaag atgggtatca
    21001 tgagttggaa atggtgatgt cgagcgtaga cttggcagat catctatttt ttgaagaaat
    21061 aacagaagac aaaatcattg tagaaacaaa taaagcattt ttaccggtag atgaacggaa
    21121 caatgtttat caagctgctt tattgctaaa aaataaatac aatatcaaaa ccggtgtcaa
    21181 gatctttgtt aagaagaata ttccagtagc agctggatta ggcggaggaa gtacagactg
    21241 tgcagctgct ctaagaggga tcaaccaatt atggaatctg ggctgttcgt tagaagaact
    21301 ggcagaaatc ggtttggaag tcggtacaga tgttccttac tgtgtctacg gaagtacggc
    21361 atttgtcggt ggcaaagggg aagaaatcca agtattacct tctatgccac agtgctgggt
    21421 tgtgttggtc aaaccaagaa tgagtgtctc tacacgtaca gtgtttagag aagtagatac
    21481 gcaagccatc caacatcaag acatccacgg acttgctgaa gccatccgag agcaagatta
    21541 tcagcagatg atcaaaaaaa tgggaaacag tttagaagat ataacgggaa agcggcatcc
    21601 agtcatccat caaatcaaag agcgtatgat caagtacgga gcagatgctg cattgatgag
    21661 tggaagcgga ccaaccgttt ttgccttgtg tcaacattat tcaagagcgc agagagtata
    21721 taatgggtta aaaggttttt gtgaggaagt ttatttagta cgaaccctgc aataaaatat
    21781 aagaagtaaa ataggtgatg aaaaactgag gaatcctaaa atggtattct tcagtttttt
    21841 attgtagtga aatggctaga tatgagcagc aactcatcca ttttacgatt taatcaagaa
    21901 tatacatgct taaatcagaa attgatggat ttctattgac tgtgtttttt tattttgata
    21961 cattagttca cgttaataaa tcgtaaaatt ccgatttgtt cattgaaagg agcatataaa
    22021 tgaaaaaata tcttttgaca gctacggtaa tgataggtgc tttactgttt gcagcatgtg
    22081 gaaatacgaa caaggaagca gacaaaaaag aggatttgac gatcgttacg actttttatc
    22141 cgatgtatga ttttacaaaa gagatcgtag gggatgaagg aaacgtgaaa cttttgatcc
    22201 ctgctggaac ggagccgcat gatttcgaac caagtgcaaa agaaagagca gaaatttccg
    22261 atgcagatgt tttcgtgtat aatagttcag acatggaatt ttttgtggat tcgttaaagg
    22321 attcagtgga tagcaaacag accttgatga tcgaagcagc taaaggaatc gaccgtttag
    22381 aatcgcaaga agcagacgaa catgaagaat cagaagaagg gcatggtcat tcacatgaat
    22441 acgatccaca tgtttggctc gatccggtat tagctataaa agaagtcaga acgattgctg
    22501 aagaattagg cgaaaaatat ccagataaaa aggaaacttt tacgaagaat gccgatgctt
    22561 atatcaaaaa gcttgaagca ctggatcaaa aatactccga tgagctaaaa gatgcaacaa
    22621 accgcacatt tgtcactcag catgctgctt ttgcttattt agcgaatcaa tacgacttaa
    22681 aacaagtagc gatttcgggc gtttcaccag atcaggaacc cacaccaagt cgcttagctg
    22741 agctaaaaga atttgtgaag aaaaacaaca tcaaagtgat ttattttgaa gaaaatgcct
    22801 cttcgaaggt agctgagaca ttgtcgaatg aaacaggagt gaagctagaa gtgttgaatc
    22861 ctttagaaag cttgactaat gaacaaatca aagcaggcga aaattatatt tctgtcatgg
    22921 aaaaaaatct gaaagcttta aaagaaagta tcaattaagt tttagaatgg aggttgtaag
    22981 gtgcgttata tcgaaatatc tgatttgacc ttttactatg atgatgaacc tgtactagaa
    23041 gatgtctctt atcatgtaga tgcgggtgaa tttgtcattt taactggaga aaatggagca
    23101 gccaagtcaa ccttggtgaa agcatcgtta ggattactga aaccttcaaa aggaatgata
    23161 aagattgctg agaaaaatca agaaggaaaa aaattaagta tcggttatat tcctcaacaa
    23221 gttgcatctt tcaatgcagg ttttcctagt acggtgatcg aattagtccg ttcaggaaga
    23281 tatccaagag gccgctggtt caagcgtctc tctgaaaaag atcatcaaca tgtacagcgt
    23341 gctttagaag ctgtagggat gtgggatatg cgaaatcggc gaataggtga attgtctggc
    23401 ggacaaaagc agcgaatcag tttagcgcgt atttttgcta ctgaccctga cttatttgtt
    23461 cttgatgaac caacaacagg tatggatgaa gaatccagaa atgaatttta tcgtcttctc
    23521 cgacatagtg cgcatgtcca tgggaaatca gttctaatga tcacccatga tcatgaagat
    23581 atcaaacagt atgcggacag acagatccga ctggtgagaa aggaggattc tcaatggcgt
    23641 tgctttcata tgaattcatg agaagagcgt ttcttgccgc tatcttcatt gccggaatcg
    23701 ccccgatgct aggtgtgttt ttagtgatca gaagacaatc gctgatggct gatacgcttt
    23761 ctcatgtctc tttagcaggg gtagcgttag gattcttttt gaaccttaat cctacactta
    23821 cgaccatgct agtcgttgta ttagcagctg tgatattgga gtatctgcga acgatctacc
    23881 gaacctattc agaaatctcc attgccattt taatggctgg tggtttggcg ctagcgttag
    23941 tattgatgaa tttgagcgga gggaattcag caacaagtat ccaatcctat ctgtttggtt
    24001 cgatcgtgac cattacatgg gagcaagtca tctttttagg aatcttattc gttgccatcg
    24061 gtatattgtt ctttttattc aaacggccga tgtatgttct gacttttgat gaagataccg
    24121 cccacgtgga tgggctaccc gttcatttga tgtcgatgat gttcaatgtg attactggtg
    24181 tggctattgc tgtgatgatc ccgattgccg gtgcactttt gatatccgca atcatggttc
    24241 ttcccgcagc aatcggtatg cgattaggca aaagcttcaa tgtcgtgatt gctatcagtg
    24301 tttgtgtagg atttctaggg atgctgtcag gactaactag ctcgttttat ctagatacac
    24361 ctccaggagc aacaatcaca ttagtcttta ttgctttgtt cctgattgta aatatcatca
    24421 aacgaatatt ggttgccttc agaagaaaaa ataactaaat tataaagaaa agaagcctta
    24481 taaaaacgaa aaatttttta taggcttctt tttattttaa taaataccga actattttct
    24541 tattttttca taaaatgctc gaatttttga agaaaaactt atataatgta aaacgataaa
    24601 atttcttaaa aggagttgga ataggtgaaa gtacgtcgca gtgaacgtct agttgacatg
    24661 acacagtatt tattgaatca cccgcatgaa ttgatttcct taacaagctt tgccgaacgt
    24721 tatgaatctg ctaaatcttc tatcagtgaa gatttgggaa tcatcaagaa aacatttaaa
    24781 gaacgagggt atggaacctt ggaaacaatc ccgggtgctg caggtggtgt attgttcatc
    24841 cctgaaattt catatgaaga ggcgaaaaaa tatattgaat ctctagcaga acgtctatca
    24901 gaacaagacc gtctgcttcc aggaggatat gtttatctct ctgatttgtt aggagaacct
    24961 gaactgctgc gtcaagttgg aagaatcatt gcttctaaat accttggtga aaaaatcgat
    25021 gctgtaatga ctgtagccac aaaaggcgta cctattgccc aagctgtgtc ctattatctg
    25081 aatgcgccat tcgttattgt acgccgggac tcaaaaataa cagaaggatc aacagtaagt
    25141 gtcaactatg tgtctggatc ttctgaaaga attgaaaaga tggaactgtc taagagaagt
    25201 ttgaaacgtg gttcacgtgt ccttgtagtg gatgatttca tgaaaggcgg cggaacagtc
    25261 aacggaatga agagcttgat tgaagaattc gaatcagaac ttgttggcat cactgtattt
    25321 gccgaatcaa aattcaacgg tcatcgcgcc atcgatgatt atacgtcact gctttatgtc
    25381 aaagatgtag atactcaaac gaaaaatatt acggttgtgc caggaaatta tttttcagaa
    25441 taagaggttg tgaaaaagcc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
    25501 tttccacatt ttttcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
    25561 atctagaggt tgtgaaatca gcgtcctgtc ctgagtatta gctcaataaa cggaaaaatt
    25621 gctcctcata ttttgccgtt tattgagcta ataccgaagg tcagctgatt gaaaaacgtt
    25681 taatgaagag gttgtaagcc taccgtttaa ctctaagtca caagaaacga tttcaaaaaa
    25741 cagcttttca tgttttatga gtagtgtgac ttgtgaccga ggagttggca ggagagcacc
    25801 ggttaccaaa tagataggcc cgttatggtc tatctatttt tttatccaga aaacgctggg
    25861 ataaaagaaa atcacttgtt ttgttgaaaa aatgggtata ctgggataaa ctgaaatcag
    25921 caaactatat ttaggagtga cgcagttgga cgcacgttat gcaattattt tggcggcagg
    25981 gaaaggtacc cgcatgaagt caaaattata taaagtttta catcccgttt ctggtaaacc
    26041 tatggtagaa catatcatta atcgggtcag tgaaacgaaa ccagatgaag tgattacgat
    26101 cgttggacac ggagcagaac aagttaaagc acaactagga gaacgcagta aatatgcgtt
    26161 acaagcagaa caattgggaa caggacatgc tgttttgcaa gctgcttctt tcttggaagg
    26221 aaaaaaagga acaacgttag ttatcagtgg cgatacacca ttattgacta ctgaaacgct
    26281 gaacaatctt tttgaatatc accaaggaaa aaatgcaagt gcaacgatct tgacggcaca
    26341 agcagaaaac ccaacaggat atggacgaat catccgtgac catattggga tcgtagaaaa
    26401 aatcgtggaa caaaaagatg caacaccaga agaagctctt gtacaagaaa tcaatacagg
    26461 aacttactgt tttgacaacg aagctttgtt cgatgcgtta agcaaagttg gaacaaacaa
    26521 tgctcaaggt gaatactatc taacagatat catcgaaatc ttaaaagaag aaggccatac
    26581 agttgctgct tatcaaacgg atgattttga agaatcaatg ggagtcaatg atcggatcgc
    26641 gttagctaaa gcaaatgaga tcatgagaaa gcgaattaac cagatgcata tggtcaatgg
    26701 cgtaagcttt gtcgattcag caacgacata catcgatgca ggcgtagaaa tcggtccaga
    26761 cacattgatc gaagcaggtg tacaaatcca aggaaataca gtcattggtt ctgattgtgt
    26821 catcgggtca cattcaaaaa tcgtagacag taggattgag gatcacgtcg tcattgaaaa
    26881 ctcagtcatt gaaagcagcc atgtcaagaa acatgcagat gtgggacctt atgcacattt
    26941 acgaccaaaa gctgaaattg gcgaaaacgt ccatatcgga aactttgtgg aagtgaaaaa
    27001 tgctcaaatc ggcaaaggaa ctaaagtcgg tcacttgact tatgttggtg atgctacgct
    27061 gggagaagaa atcaatgtcg gatgtggtgt tgtcttcgtc aactatgatg gaaagaacaa
    27121 acatcatacg actgtcggcg accacagctt tattggttct agcacgaata tcatcggacc
    27181 tgtcgaggta gctaaaaaca gttcgattgc tgccgggtca acaattacag acaatattcc
    27241 agaatatgcg ctggcaattg cgagagcaag acaagtcaat aaagaaggat atgcaaaaaa
    27301 actcccttat ctaaactaag acttttgcaa aatcacttga tttatttaaa aaatatgact
    27361 actatttatt aggtagaatt ctagagttgt gaaaaagaaa gtggaggtcc ccatgtcaaa
    27421 acattacttt gacccaagat taaaaatttt tgcgttaaat tccaatcgcc cattagcaga
    27481 gaaaattgca gcagcagtcg gcgtcgagtt aggaaaatca tctgttactc aatttagtga
    27541 tggagaaatt caagtaaaca ttgaagaaag tattcgtgga tcgcatgtct atgttattca
    27601 atcaacgagc agcccagtca atgataactt gatggagttg ctgatcatga ttgacgcact
    27661 gaaacgagca agtgcaaaaa caatcaacgt agtcatgcct tattatggct atgcgcgtca
    27721 agaccgtaaa gcacgtgcac gggaaccaat cacagcgaaa ttagtagcaa acatgattca
    27781 aaaagctggt gcaacaagaa tgctgacttt ggaccttcat gcagtccaaa tccaaggttt
    27841 ctttgatatt ccagtcgatc atttgatggg tgcgccactt attgccaatt atttcttaga
    27901 aaaaggaatc aaaggcgatg atgttgtcgt tgtttcacca gaccatggtg gtgtcacacg
    27961 ggcacgtaaa ttagcggaat ttttgaaatc tccgattgcg atcatagaca aacgccgtcc
    28021 aaaagcaaat gttgcagaag taatgaatat cattggacaa gtcaaaggca aaacttgtgt
    28081 actgatcgat gatatgatcg atacagcagg tacgatcaca ttggctgcca atgctttgaa
    28141 agaagctggt gcaactagtg tgtatgcttc atgtacacat ccagtattat ccggccctgc
    28201 tttacaaaga atagaagatt cagctatcga gcgtctagtt gttacagact cgatctatct
    28261 tccagaagat cgtaaaatcg acaaaatcga tgaagtaagt gttggcggac taatgggaga
    28321 tgcaatcaaa cgtatccatg aaaacaaacc agttagtcca ttgtttgaaa caaaaaataa
    28381 ataataaata aactcacaaa ggggagtcca attgggctga gattgaatct atttctaaac
    28441 ccttcgtacc tgtatcggtt atgcgagcgt aggaattgtg aataaacagc tgtttgacta
    28501 ttttcgaaag ctgcttcctc ctttgtgact tttttattca aaggaggaag tttttttatg
    28561 ggaaaaaata gagtttgggt agaaggaacg attgttgcag cgttagcgat ggtgttgtcg
    28621 ttgattccga tccagatcgg cagcagtttt tctatttcat tagggcagat tccattgact
    28681 ctttttgcgt taagaagagg gacaaaggca gggatgctgg caggtttcat ttggggcatc
    28741 ctacattttc cgttgggaca agtttattat ttaagtgtcg ttcaagtgtt gattgagtac
    28801 ccgattgcct atacttttgc aggcgcagca gggctcatgg cttcaaatgt ccaaaaaggc
    28861 tatgtagacg ataatacgaa acaaattaga aaaagcatcg tgtttggtgc attgattggc
    28921 gcactttcca gatatttttg gcattttatt gcaggagtgg ttttctgggg agcgtatgca
    28981 ctatggggga tgaatccttg gctcttttct ttcgtaatga atggtgccag cgggttgagt
    29041 acagctattg taacgatcat cgttttatta gggatccaac aaacgactcc ttcgattttt
    29101 gtgccagata aacgacagga gctgtaaata aaaaagaagc aagaaacttg agacagaatc
    29161 atgtcctagt ttcttgtttc ctttttattt tttgataaga aatagatcta cttcaatggc
    29221 agataagaaa gatcggcatc ttctaccaat tgccagtgtc gatcggtgac agagtagatt
    29281 aaagtagcga tgatttcctc ttctcgatac cgggtgcctt ttaatttcca aatataatct
    29341 tcaatcacat agattgggat acgatattta ccatcgatca aaccaacttg atcaaaagaa
    29401 aaaatgatcc cttcctgcat caaattacgc atttctttca ataattccac actgattttt
    29461 tcaacaggtg tttctttttt gaaaaaagat ttttttacat tgatcttatc tgacaagatc
    29521 gaacttaatt tactagcaaa aatcctataa aattcatcta aatagcgata ataaatccga
    29581 ttcagctgat cattaagatc gaagtataca aaattattct gtagtttata aaaaaacggc
    29641 gaatgaagat gtgttttcat atgaccaaaa tataataatt ctgaaatttc taaaggtgtc
    29701 aattctttca gcatcgttaa atcagtaaag tcgatccact tcaattcatc gaatccacgc
    29761 ctgttacgag aagaggaaaa gaaacgctga atattttctg taccacggat tactttcagc
    29821 cctgtatggg attcatattc tccagcagaa gcagatggat caagcaagag caagttttga
    29881 ggaaaatgca cgatactgtt tgcaaagtca gtagcagtca gcccttttgt taaaacagca
    29941 ttacttgtgg tatcaatatg tacataaact gactcagtca tcatgttcct cctcttattt
    30001 cctcttaatc tcttttattt tacaacattt gcattaaaaa agcttgtttc ttttatttta
    30061 aaattccatt acaaaatcac gttttcattg aaaacactct cattttcaat taatttttgt
    30121 gatttttgta tttttttatt taaaggaggt tttatgaatt gttttcttat ggaatctaca
    30181 aagtcgaata cgagactcat gaccttttta tgacgtactg gtatacttga agaaaaagga
    30241 gagcgcgatg gagataaaaa aagtttactt ttcacagaaa atgtcgctgt attcgcctat
    30301 ccagcaagca aaacaacccc ttccattttt tcaaagtcat caagaaagta gagacgcttt
    30361 ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta tcgaaaaaat ataccgtcta
    30421 ttttactatt gctaaaagta gaggagatgt tatttttaat aatctgttga ttgaaggtga
    30481 aacttttgaa tggaaaaaat tttttcggta tatggaagct tgcgctcgtt tttttattaa
    30541 agaaaactgt tgttttttat ttcaatctcc tctatcagaa gaatggttgc gtatttttca
    30601 taaaaacggc tatcaaggaa ccacacaatt aaataaaaag ctggtctatc atacagcact
    30661 tgttttaggt ggaggaggag cgcacggtgc ttatcagatt ggtgtgtggc aggcgctgaa
    30721 ggaacatgat atcaactttg agattatcac aggtacctcg gtaggtgcgt tgaacggcgc
    30781 attgatttta caaggagata tgaaaaaagc tgtgaattta tggaaaaagc tgtcgacaag
    30841 acaagtgctt gcgttaccag aaatggctgc agttgaagac cttcgtgaac gcttctatcg
    30901 agaaagacgc caaatgacga aaacagcact aattgaaggc ggggtttctt ctgctccatt
    30961 ggaaaagatg gtcaaagaca atttggattt gtccttactc aaacataatc ccaaaatacg
    31021 tttatatacg gtgtctacta aactgccaga actagcggaa gtagtgactg atatccaaca
    31081 gacaaaaaca gaagaaatac cagattggat actcgcctct gcttcatttt atcccgctat
    31141 ggcttatcga aagatcggaa aaaataaata tgcagacggt gggtatcgca acaaaatacc
    31201 tattgatatt gcgattaaca aaggagcaac agaggcattt gtcgtagatg tgcaagggcc
    31261 aggcccagcg aaaaaaattc gtatgccaga catttttatc cattggaaat gtcaaacatt
    31321 gtggacacta ggcagctttc tgctttttga tagccaaagg aaccaattaa atttacaatt
    31381 aggctatctg gaaatgaaaa aacgattagg ctattactat gggaactggt atacatttca
    31441 ttctgttaaa cctgcaagaa catactggcg aggatttttg tcttacttaa caaaagaaat
    31501 tcagttagaa ttatcttttt tcaaaagcat aaaattttgg caaaaactgc gtaatttata
    31561 taagaacagg gtcgtaccag agacatgcgg attggcgatg ctagaacttt tagcaaaaaa
    31621 acatttttta ctgcccaatg aaatctatca agtagacatg atgattcaaa tgatctgcca
    31681 acaagatata aaaagtatgc cggatgattt attggcacaa attggtcaat taagtactga
    31741 agaatggcga aggtatcgaa aataccagca aaaaatacaa aatgagcgca cgaaaactgt
    31801 ttattttgat tatctgctac aaggaaaacg caaggatcgt ttgcagcatg aattgcttaa
    31861 gcaaccagtt gacacattac tgatcctata tttatattat ctcaaggagg aacaaccatg
    31921 gcacaagaat tttcatatga aatcgtagaa gaaatcgcta tattgtcaga aaacaacaag
    31981 gggtggcgta aagaactgaa cctagtcagc tggaatggtc gcccgccaaa attcgatctt
    32041 cgagattggg cgccagacca tgaaaaaatg gggaaaggcc tgacattgac gaacgaagag
    32101 tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag gaaggaccct gtcgaatgaa
    32161 aagtatgact ggttttggca aagccatccg cgaaacaaaa gcgtatcagt tagatattga
    32221 aataaaaagt gtcaaccagc gttttctaga tgttcaaata cgaagtccta aaatcctgaa
    32281 ctttgttgaa aacgattttc gtcagcttat taaacagcag ttaagccgcg gtcgaatcga
    32341 agtatttgtg aatctagctt atattggcaa aaacgaaaaa cagatcgtca tcaactggga
    32401 attgattgat tcacttatca ctcaattgac agacggaatt caacaacgat acggtagtca
    32461 aaatcaagtg tcaattagtc ggatggcgga acgcttcgtg ctggacgaaa acttcgtaac
    32521 ggtagaagaa aaaccggaag agagcatgga agaattggca cagttagctc tagatacatt
    32581 gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa gaaggtcaag ctttagcttt
    32641 agtgttgaaa aagaatactg aagaattcaa agaagtacta aaaaaactcg atagttttgt
    32701 agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa aagctggagg aatttcttgg
    32761 agcgaaagtc gatgagcaac ggttgttgac ggagctagcc atcttgttgg aacgcggaga
    32821 tattcacgag gaactggatc gtctggcgat ccatgtacaa aaactagatg aacttatcga
    32881 agcacaatgt cctgtaggtc gggaattaga tttcctgatc caagaaatga atcgggaaat
    32941 caacacgatc ggttccaaat caaatgcaat cgaaattaaa aatcaagtcg tccagtctaa
    33001 gacgatctta gaaaaaatca gagaacaagt acaaaatatt gaatgacgaa agaggtagag
    33061 ataatgaaat attatgacgt aacgttccac gaattaagtg gaaaaactgt ggttaaacgg
    33121 gatatccctt ctgaaaaaaa cggctttgac gtatggaaag atgcctgtgc agactataat
    33181 gagaacgaac tgtttatttt gatcaacgac ggtgcttacg tgacgatgaa tcggaaattt
    33241 attgttcgaa ttgacacaga agaggtagaa gatccgacag aaaaagcacg ttctcgaaaa
    33301 gatgagatca tgggtgtagt gaatactttg tccaatatgg gcttttagca aggatgctga
    33361 acaaagaaat acaataaata aacagaattg gatttcttcg cttagcttct ttgctctagg
    33421 aatacattta aaggttgtga caaaattcgt gtcacaatct ttttttattt tgaataagtg
    33481 atgttcaaaa accattttct cggtattaag ccaaatttat gcaaaaatgt gagaaactat
    33541 tttcacaaaa tttttcttca tactcgaaac taatcggctt cttcacgacc tcttatacac
    33601 ggcgttcaaa agcttgcatc tgcgataata accccaacca aacgaaaaac atgagaagct
    33661 gtttatgttt ggctgttctt attacttgat gcaggacagc ttttttacga cctcttggta
    33721 cagggtagct tttttaccaa tttctagcta taattaaaga taaccttgaa agttgcagaa
    33781 aaaaagtgca aaataaacga taagtacaaa aagactagtt gaaaggacgt cgtcatgtca
    33841 gagcgtggat tattaatcgt gttgtctggt ccatccggtg taggaaaagg cacagtacga
    33901 aaagcaattt ttgagagtga ggataatgat tttcagtatt cgatctcaat gactacccgt
    33961 aaaatgcgtg aaggggaggt cgaaggcgtt gattatttct tccgaagcaa agaagagttt
    34021 gaatcaatga tcgaagctgg agagatgctg gaatacgcag aatatgtcgg gaattattat
    34081 ggaactcctt tgtcatatgt caacaagaca ttggatgaag gaaaagatgt tttcttggag
    34141 attgaagtcc aaggagcaaa acaagtcaaa gaaaaagtgc ccgatggtgt attcatcttt
    34201 ttgactccgc cagatttagc ggaattaaaa tcacggatcg taggccgagg aacagatgca
    34261 gatgaagtaa tcgaaaaacg aatgaaagtt gcaaaagaag aaatcgaaat gatggctctt
    34321 tacgactatg ctgttgtaaa tgatgaagtg ccgctagcag ttgaacgaat caaaaacatt
    34381 attgccagtg aacattttcg agtagatcgc gtaatcggta aatatattaa aatgttaaag
    34441 gagatgtagt cttatgatgt taaaaccttc aattgactca ctattagatc gtgtaaattc
    34501 aaaatattct ctagtgattt tagcaagtaa acgcgcgcat gaattggatg caagtgcaca
    34561 gcctactcta gattcttttg attcagtaaa aagcgtcggt caagctttag aagaaatcga
    34621 tgcaggaaat gtagtcaatg atccacatcc tgaattaaaa cgtgaacgtt tgaagatgga
    34681 agaagaggaa cgtcaagcac aaaaagagcg tgaacaacac gaattggaat cacgtatccg
    34741 tgaagagcaa aaaatgtaat aaagaaaaat agagcgaaca tctaaaaaga aaagcaaacc
    34801 cagctgtttc cttttcaaac attcgccact tattgtctgg tagaaaaggc tgtgtctgaa
    34861 ctttttgaca cagttctttt ttttcttatc tgctgttaat tttttgttag gatttggcag
    34921 aagataagac aaatccatcg atctctagta aaataggcaa ggaacttttt gtcgcttgat
    34981 aagaaaagac aaaaaaagga ggaaacgaac catgacatta acagctgaag tcattgtgga
    35041 cgtacctact atgcagaccg atcagccatt cacttatttg gttccctctg aaatggaaac
    35101 agctttgcaa gtcgggatgc gagtagaagt cccttttggt aatggcaatc gtcatgtcca
    35161 aggatttgtt atggctatcc aaaaatcaga agaatcagct aatccatctt tgaaggcagt
    35221 tattcgtttg ctagaccttg ctccggtggt caatgaagaa ttactttcat tagctgatta
    35281 tatgaaaaaa attacttacg cattcaaaat cacttgtcta caaacgatgc ttccgagtgt
    35341 gatgaaagca gaatatgata aattgatcta tcctttggca gacacaccag aagttcaggt
    35401 attgtttgat cagcgagaag tgatttcctg gaaggaagca gaagaagaag gcagcctttc
    35461 tcaattgatt cgctggcgcc aagagcaatt agtagatatc aaatatgaag tccatacacg
    35521 aaataaagta aaaacgattc gtttggtccg ttctttgtta actgaaaagc aaatcgaaga
    35581 agaatgggca aaattacggc aaaatgccaa gaaacaaaaa gaacttcttt tgtgtttatc
    35641 tgaactgagc caagaagaac caattgctta tttcaaaaat aaaggaatca gtactgctgt
    35701 cttgaatcaa ggaaaagaaa agggctggct ggagtttata gaatcagaac gttatcgcga
    35761 cccttataaa gaccgagtat ttgaccaaac gacggcattg gaactgaatg cagaacaaaa
    35821 aaatgctgtc gaacaaatca tcacagcagg tcagcagcaa aaagatcagg tctttttgct
    35881 ggagggaatc actggcagcg ggaaaacaga agtatatctt caggcaatcg ctgatgtttt
    35941 gtcagaaaat aaaacggcta ttatgttagt gcctgaaatc gcattgactc cgcaaatggt
    36001 cgagcgattc aaaggacgct tcggagaatc agttgcggtc ttacatagcg gactttccca
    36061 aggtgaaaaa tatgatgaat ggcgcaagat tgagcgggaa gaagcgcaag tagtagtagg
    36121 cgcacgatct gctatttttg ccccgctgaa aaatatcggg ctgatcatta tcgatgaaga
    36181 acatgaatca agttacaaac aagatgagac accgcgttat catgcgagag atctagcaat
    36241 ttggcgcagt aaatatcatc attgcccaat agttctagga agcgcgacgc cctctttaga
    36301 atcaagagca cgagctcaaa aaggcgttta tcagttattg cagttaaacc accgagccaa
    36361 agcagctgca cagcttccag ctattgagat cgtagatatg cgtgaagagt ttcagaatca
    36421 tcggacctct actttttctg ctaatttgca ggaaaaaata caaaatcgat tagacaaaaa
    36481 agaacaaact gtcctactgc tcaatcgtcg aggatattcg tcttttgtga tgtgtaggga
    36541 ctgtggattt gttcttccat gtcctaattg cgatatctct cttacactgc atatggatac
    36601 acgttcaatg cgctgccatt attgcggaca tgaagaacct attcccaatc gctgtcctaa
    36661 ttgtggcggc aataaaatcc gctactatgg aacaggtact caaaaagtag aagaggaact
    36721 ccgagaactt tatccgcaag ctaggatttt gcgtatggat gtagatacca ctcgaagaaa
    36781 aggggcacac gaacagattt tacaaaaatt cggtgcaaaa gaagcagata tattattagg
    36841 gacacaaatg attgctaaag gtttggactt tccagaagtg acattagttg gggtgttgaa
    36901 tgctgatacc tccctgaatt taccggattt tcgttctagt gagcatacat tccaattgct
    36961 tacccaagtt tcaggacgag cagggcgagc ggaaaaagca ggcgaagtcg tgatccaaac
    37021 attcaatcct caacattacg ccatagaatt ggctaaaaaa caaaattatg agcagtttta
    37081 tcagcaggaa atgcatgtac ggcatcgtgg aggctatcct ccttattact ttactgtgaa
    37141 aataaccgcc agccatcctg aagaacaagt agcggcaaag aaaatctttc agattgccaa
    37201 tcagctaaaa gaagtgttaa gccctcaaag cttgctatta gggccaacgc ccagcatgat
    37261 tttgagagtg aaaaaccgat attattatca actaatcatc aaatataaac atgaaccgaa
    37321 tcttcctcat gttttggatg aaattttgaa tggaagccag aaagaacagc gacaaggtct
    37381 ttttgtggca attgacaacg aaccattaaa ctttatttaa gaaagggcca aattatgcgc
    37441 tatcctatat tgatccaccc taatgataaa ttaaaaagaa ctgctcagcc aatcgatgta
    37501 atcacggacg agacaatcgc tttattagac aatttatatg agacaatgat tgcgaatgac
    37561 ggaataggga tcgctgctcc tcaagtagga caaaacaaga gaattgctgt gatcgaagta
    37621 gatgaaggcg agaaatttga actgattaac ccagaaatca tcgaagcaaa aggtgaaagc
    37681 cttgacgtag aaggttgtct cagtattccg catgtctatg gaacggtcaa gcgagcagat
    37741 gaagtgaccg tgcgttatta tgatcgagac ggagaagaga tagaagtgac ggcttttggc
    37801 tatttagccc gtgccttcca gcatgaaatc gatcacttag acggtatctt gttcattgag
    37861 aaaatgatac agcagatccc ggaagaagaa cttgaagaat atatggagga acatcttgat
    37921 gactaagcta gtatttatgg gcacacctgc tttttctgtg ccgattcttg aagggctgat
    37981 agaatcaggt tacgagatcc aggcagtggt aacacagccc gaccgtccag ttgggcggaa
    38041 acgagtaatt acgccgacac cggtaaaaaa agcggctctg aagcacggta tccgtgtgct
    38101 gcagccagag aaaatctccg gttcgccaga aatggaagaa atcattgaat tggtgcctga
    38161 tgtgatcgtc actgcggcat ttggccaatt cttgcctgaa aagttattgc aagtaccaaa
    38221 attaggggca atcaatgtcc atgcttcttt acttcctaaa tatcggggag gtgcgcctgt
    38281 tcattatgcc atcatgaatg gggagaaaga aactggtgta acgatcatgg aaatgatcaa
    38341 aaaaatggat gcaggcggaa tctatgcgca agaaagcata ccaatcacta aacaagatga
    38401 tgtagggacg atgtttgaaa agcttagcct tttaggaaga aagctattgc tagaaacatt
    38461 gcccaatatt ttggatggac aaaaaccagt acctcaagac gagtcagaag caactttttc
    38521 gccaaacatc acacgggaac aagaagcaat cgattggcat aaaactgcag aagaaattga
    38581 caaccaagta cgcggaatgc gtccttggcc aattgcattc acgacctatg agggcatacg
    38641 ctggaaatta ttatcggttg aagtattggc agaaactaca gacaaagcac ctggtacgat
    38701 catccggaaa gacaaaaaaa atctatggat tgcttgcggt aaacagaccg tcttagccat
    38761 caatgaatta cagccagctg gtaaaggaaa acaggccgta catgatttcc ttaacggaag
    38821 cggtcagcaa gttcagattg gtcagcaggt gaaataatga aaaaaatccc taaaaaaatg
    38881 aaaaaatcag tacgatatat ggcattactg acattagagc gagtgaacaa gggtggcgcc
    38941 tactcgaatc ttttgttgaa tgagatgatc aaacaaagcg agctaaatca aaaagatgtg
    39001 ggtttattta cagaattgat ctatggtacg attagccatc agcgactatt ggaattttac
    39061 gttgcaccat tgatcgctaa agcaaaaaaa gtggatgact gggtcaagat gcttctttat
    39121 ctttctgtat accaattaga atttttagat aaagtccctg cacatgctat tttaaacgag
    39181 gcggtcgaaa tcgcaaaagt aaaaggaaat cctgggacag ggaaatttgt aaatggtgtc
    39241 ttgaggaatt atcagcgtca aggagcacca gatctgcaag gcattgctga tcctatcgaa
    39301 agactttctg tcaaaatcag cctgcctcta tggatgacga aacgactggt ggatcaaatc
    39361 ggatacgaag aaactgaaaa gctcggttta tcgctatatc agcctagcca tgctagtgcc
    39421 cgagtagata cacgccgact ttccagagat caggccatca ctgttttgca agaagaagag
    39481 atcacagctg aaaaaagcca agtcagtcca tacggtatag tagcgaaaaa agggcattta
    39541 gcttctagct cattgttcca cgatggcgtc atgacgatcc aagatgaaag ttcgatgctt
    39601 gtagcgccgt ctatgcaaat cgagcctgaa catcatgtct tagatgcctg tgcagcaccc
    39661 ggaggtaaga cgacgcatat tgcttctttt ttagatccgt catgcggtgg agaagtggtc
    39721 gctttagatg tacacgaaca caaagtcaaa ctcattgaag ataatgcagc acgtctaggt
    39781 gtctcttctg ctgtctacgc tcaaaagatg gatgctagag aagtcagtaa acagttcgaa
    39841 gcagagcagt ttgatcggat tttagtagac gcgccttgtt caggtcttgg gctgcttcgg
    39901 cgaaaaccag atattcgtta taaaaaacag ccggatgaat tagcgaattt accaaaaatc
    39961 caattggaaa ttttagaaag ctgcgcacca actttaaagt cttccggtat tctgacttat
    40021 agcacttgta cgatcttaaa agaagaaaac caagacgttg tttctgcttt cttggaaaga
    40081 caccctgatt ttgagaggat tgatgttttg accaatccta tgctacaatc atcaatcgaa
    40141 gagaaaatgt tgactttgta tccgcatcag ttttacacag atggattttt catttgttgt
    40201 ttgcgcaaaa agtaatgagg tgaaaaaatg cagattgaat atcaatcaga tgtaggaaga
    40261 cgacgtaaca caaatcaaga ctatgccagt gtatttacca gccgttcagg tctcaagctt
    40321 gctgtattag cagacgggat gggtggtcat cgtgccggag acattgccag tcagatggcg
    40381 gtaacgaatc taggcgaagc ttgggaaaaa caagaagctg ttgacgatga aaaaatcgcc
    40441 caatggttca ttcaatcgat tcaagaagaa aatatgctga tttatcagcg tggacaagaa
    40501 cagccggaat ataacggtat gggaacaaca atcgtagcgg ccgctctgtc agatgactgg
    40561 ttcacgattg ccaatgttgg ggatagcaga gcttatttga ttcgtgaaga taaaatcatg
    40621 caattgacgg aggatcattc tttagtcaat gaactagtca aatcaggaga aatcagcgaa
    40681 gaaatggcag tgaaccatcc aaggaaaaat attttaacac gttctgtcgg catgcctgga
    40741 acagtcgaag tagatgtttc tactcatctt tggcaactaa aagatcgact gctgttatgt
    40801 tcagatggct tgacgaacat gctgtctgaa gaagaaatag gaacgatcat caatcaagaa
    40861 ggcccattgt ctcataaagt aactgagctg attgatcgtg ccaatgaagc aggcggagcg
    40921 gataatatca ccgttcttct tatcgaatat aaggaggaca gtgtatgatc gagttgggca
    40981 aaaaattaaa tggccgttat caaatcactg gaaatatcgg tagcggtggt atggcaaatg
    41041 tatttttggc gcacgattta atcttggatc gtgatgtagc agttaaagtc ttacgatttg
    41101 actttcaaaa tgaccagact gcgattcgac gatttcagag agaagcttta gcagcaacag
    41161 aactcgttca tccaaacatt gttagtgttt acgatgttgg tgaagaagac aacatgcagt
    41221 atctggtgat ggaatacgtc aaaggaatgg acttgaaacg atatatccaa acacattatc
    41281 ctgttcctta tgaaacagca gtaaatatta tgcagcagat cctttctgct atttctttag
    41341 ctcataacca tcaaatcatc caccgagatt tgaaacccca aaacgtactg atcgataatg
    41401 aaggtgttgt aaaaatcacg gatttcggga ttgctatcgc tttatctgaa acatcgatca
    41461 cgcagacgaa tacgatgcta ggatctgttc attatctgtc ccctgagcag gccagaggaa
    41521 gtatggcaac gaagcagtca gatatttatg ctttagggat catcctttat gagatgttga
    41581 cgggtagcgt accgtttgat ggtgaatctg ctgtaacaat tgctttaaaa cattttcaag
    41641 atgatcttcc atctataaaa gcacttgatc caaatgtgcc acaagcactg gaaaacgtga
    41701 ttttacgggc aacggctaaa gaacctgcag accgttataa gtcagccgag gaaatgtctg
    41761 atgatctttc tacagtatta tcaccagctc gtgcaaacga agaaaaatgg cagcctcatg
    41821 tgatggataa tgaaacaaaa gttatcacac cccttacgga agatgcacca atgcctgatt
    41881 cattcaaatc tatgcctctg ccaaaagaca agccagaaaa cgaaacctta ccagaggagg
    41941 aagaagttct gcctgatcct aaaaagaaag gacagaaaaa atggtggctg atcctattag
    42001 ctattcttgc tgttttagga atcggaacag ctgtattctt tgcttctggc gggcgaggag
    42061 aagttgttgt tcctgatgtt tctgatcttt ctgaagcttc tgctagagaa acactcgcaa
    42121 aagcgaaatt agaagtagca acaaaaacag aagaaattgc agatgacaaa atcgaagaag
    42181 gtaatgtagt gaagacagac ccggcagcgg gaacgacagt caagcaaaaa cgcgaagtca
    42241 ctctgtatat tagtagcggc acgaaaaaaa tcaaattaga tgattacagt ggtaaaagtt
    42301 atgaagatgc cgaagcaagt ttgaaagaat tagggttttc tgctgacttg attcaaaaag
    42361 aagaggaatt cagttcagag gtagaaagcg gaatgatcat ttcgcaatct ccatcagaag
    42421 atacagaagt cgatcctaaa acagacacga ttactttcac agtcagcaaa ggaccagaaa
    42481 cagtgacttt gagtagtcat gtaggagaaa actacgaaac tgttcgtaat tggcttgtta
    42541 cacaaggatt caatatcaat aatattagtg ctacttatga ctatagcgac actgtaccat
    42601 caggttcgat catcagtcag gatccagcct cgggtgaagt tgtagcagaa gaaacttaca
    42661 tcaattttgt cgtaagccaa ggaacagagc ctaaattgaa tgatatctcc ggctatacaa
    42721 aaagcgaagc acaaagttat ttagctagtg tcggtgcaga atatatcggg catgagactt
    42781 ccgaatattc aaacacggtg gataaagata aagtgattcg tacagtacca ggggcaggaa
    42841 cgacaattac caaaggaatg gttgtgaacg taatttactc taaaggacca gatccagcgt
    42901 cttctagttc tgaaaaagac tcttcagaca gttctagcag ctcaagtagc tccagtagca
    42961 caagcaactc ttcaaaagaa gaatcttcca gctccacaag ttcatcagct agcacctcag
    43021 aaagttcaaa ataagtgctg aataaaatat atcagaaggg aatgcgacat tgatttgttg
    43081 cattctcttt tttgtgtttc tatctctttt gatcaatttt caagccatat actttgagaa
    43141 gtttgcatgg aataacacct actttcctct tcgtatggta aaataaacaa aaacggaggt
    43201 ggcacttatc aaaggacaaa tcagaaaagc aatcagcggt ttttactatg tatacgcaaa
    43261 cggagagacc tatcaaacaa gaggaagagg aaatttccgg aatcggaaaa tcacgccgct
    43321 tgtcggagat caggtgatct ttgagagtga aaatcaaaca gacggctatc ttctggaaat
    43381 cttgccgaga aaaaatcaat tagttcgccc accagtcaca aatgttgatc ttggaatcat
    43441 tgtcacaagt atcgtagagc ctgcattttc ttataatcta cttgaccgct ttctagttac
    43501 cttggaatac gagtcgatcg aaccgattat ttttttgaca aagaccgact tgtcagacga
    43561 cccagaaaaa atcgaagaga tcaagcaaac ctatactaaa ataggctatc cggtgattgt
    43621 acccaatcat acgggtgata cgacagaact gatccgttac ttcccagaac ggctatcggt
    43681 ctttatgggt cagtctggag caggaaaatc gacgctactg aatcaaatat caccggaact
    43741 gaatttagca acaggagaga tttctgatgc acttggaaga ggacggcata caacacgtca
    43801 tgtggaactg attcctatct atggcgggtt agtagcagat acaccaggat ttagctcgat
    43861 tgactttttg acaattgaag caggagaact gccgagacaa tttcctgaat ttgtagaagc
    43921 ttctgtccat tgcaaattta gagagtgcat gcatgctaaa gaacctggct gcgaagtaaa
    43981 acatagagta gaaacaggtg gtatcgcaca gacacgttac gacaattatt tgcaattctt
    44041 acaagaaatc gaaaaaagaa aaccaatgta tgcaaaaaaa gaaggtaaac gaaaatagga
    44101 ggaaacaaga atgaagatag caccatccat tttgagtgca gattttgcca atttacaaag
    44161 agacattgag ttagtggaaa aaggaggcgc agattacatc cacgtagatg taatggatgg
    44221 ccgatttgtc ccaaatatca cattagggcc aaatattgtt caggcaatcc gtcctgttac
    44281 taaattaccg ttagacgtcc atctgatgat cgttgatcca gaaaactaca ttccagcatt
    44341 tgcaaaagct ggagcagata tcattactgt ccacgtcgaa gcaactcctc atattcatcg
    44401 ggcacttcaa atgacaaaaa gcttaggagt taaagctgga gtagttatca atccaggaac
    44461 accagtctcc atgatcaaac atgttttgcc tttggctgat caagtactag tcatgactgt
    44521 caatcctggg ttcggcggac aaagctttat tgaagaaaca gtagaaaaaa ttacggaact
    44581 cgctgatcta cgtagagaga acaattggac ttatgcaatc gaagtagatg gcggaatcgt
    44641 tccagaaaca gcaaaaatct gtaaagatgc aggggcggat gtcttcgttg caggatcata
    44701 tatctatgga gccgaaaatc cagtcggaca aatcgaacga ttaaaagagg cattagacta
    44761 aatgaaaatt ttattagctg ctggaggtaa tccgcaaaac tggccgcagt ctctgctgtc
    44821 caaatacgat gcctatgtcg ggatagaccg tggtgcgctg tacctgcttc aagcaggcta
    44881 cccagtagat ctggcagtgg gtgattttga ttcattgtcg aacgaagagc agaaggaagt
    44941 atttgcaaaa gccaaagagt atacccgttc ccctgcagaa aaagacgata cagatacaca
    45001 gttaggtatc ttaaaaacat ttgaacgtta cccgcaagcg gaagtcactt tgattggagc
    45061 tacgggcgga agattggatc atcttttagc caatctctgg ttaggtatgg aacctcgctt
    45121 caaacccttt gcccatcagc ttattatcag agattgtcaa aattatttgt cttattattt
    45181 gcctggagag catatagtaa gacagatacc agatatgaag tatttagctt attgctgctt
    45241 gactcccgtc tcgaatctta cattaaaagg cagcaagtat gtattggaaa ataaagaagt
    45301 agaatatccg acatcttatg caagtaacga atttctttca gataccgcag aattttcttt
    45361 ttcagatggt atgattgcag ttattcaaag caaagattga tccataaaca aataaaaaaa
    45421 tttgtctttc aggctaaaaa tgacagaatc tattatttgc taaaaaaaag cttaagagga
    45481 gcaacttacc ttttgtttca cggcatttag tgataaaatt gaacacaaga gtctaattaa
    45541 ttcgaggtgt ctttgtgaag aagttcaatc ctaataaaaa tattattatt accttggtta
    45601 ttgttatcat cgtggtgacg atactaagtg tgaccgctgc gcagcgagcg aatgaaggaa
    45661 agcccaattt cttccaatcc atcgttaatg acagtgttgc aatggttgat aaaacaatct
    45721 ctgccccagt acggtgggta cgaaatggag tagattctgt ccataatctt ttttcaactt
    45781 atgccgaaaa tgagcggcta aaagaaaaaa tcgatagcta tgatgacgtt tcacagcaaa
    45841 acaagaatta cgagagagaa atcgaagcgt tgaaaaaaga acttgagttg aatcagacgc
    45901 tgactagtta cgaaaaagta acggcaaatg tcattactcg ttcgcctgac agttggcagg
    45961 acatgctgat cgtagacaag ggttctaaag acggtatcga agcaaatatg gcagtcatgt
    46021 cgcaaaaagg attgattggc cgagtgatcg aggtcaatac ggcttcgtct aaaatcgaat
    46081 tactgtcatc ctctaatgaa agctccaatc attttccagt acgggtatct tcggctaatg
    46141 gcgaagcgtt tggtttgctt aaaaactatg atgaaaagct ccatgcctta gtggtgaccc
    46201 aattaactgg tgatacggat atcaaagaag gggatgttgt ccagacatcc ggtcttggag
    46261 ggaattctcc agctaacttg ccgatcggta cggttattaa aacgaaacca gatagttatg
    46321 ggctggatcg ggaagtttat gtgaaacctt atgcagaaat gtatgacgtg tcagttgtga
    46381 cgattgtcca acggctagcg gaggattagt ccatgttgaa gaaagaaaca atgaagtact
    46441 atctgccaat cgttttgttc tttttgatgt tgatagatgg tcatttaaca agaatgctag
    46501 gggagtggtc gaaaggcacc tatatgtcaa atgcccactt tctgatattg gcattattat
    46561 gttgcagtat ggcgtttgaa aaacgttatt tactgattac cacgattgtt ctcggggcta
    46621 tctatgacgc ttactatatt ggcgttatcg gtatctatgc agtagctctc cctttaattg
    46681 tatggttgat gtatgtaatg aaagacgtta tccatgtcaa catctttact gaatttttca
    46741 gtatgatcat ctttgtcacg ggttatgaat tgtttacgat ggtggtccag ttgattttta
    46801 aattagcagt agtaaataac acgtatttta ttacaaggtt tttaggacct acactgctgt
    46861 tgaacatgat tatatttgta ttattcattt ttccctttaa gaaattattc agtgatgaat
    46921 aatttctttt tttattttta ggtcgaaagc gaaaaagaat cgttgttatc ttaagaaatg
    46981 ctagacttaa cgcggctttt tcatgatcat tttctgaaac gaacagtttt gtcattttta
    47041 aaagttgttt tgaaacattt ttgtaacata actattatat cgttgacaca ctgctatggt
    47101 acaattttta tatcgttaag aaagacttaa atatatttag atatttatta aattaagcca
    47161 gtcggaggaa tgaagagtga aaaagagttt aatatcagca gtaatggtat gttcaatgac
    47221 cttgactgcc gtagcatcac cgatcgctgc cgcggcggac gattttgatt ctcagataca
    47281 acagcaagat caaaagattg cagacttgaa aaatcaacaa gcagatgcac aatcacaaat
    47341 cgatgcctta gaaagccaag tttctgaaat caacacacaa gcacaagacc tattagcaaa
    47401 acaagataca ttgcgtcaag aatctgcaca attagtaaaa gacattgcag atttacaaga
    47461 acgtattgaa aaacgtgaag atacaatcca aaaacaagca cgcgaagcac aagtaagcaa
    47521 cacaagctca aactacatcg atgctgtatt gaatgctgat tcattagcag atgctattgg
    47581 acgcgttcaa gcaatgacaa cgatggtaaa agcaaacaac gacttgatgg aacaacaaaa
    47641 acaagataaa aaagctgtag aagacaaaaa agctgaaaac gatgctaaac taaaagaatt
    47701 agctgaaaac caagctgctt tggaatctca aaaaggcgat cttttatcta aacaagctga
    47761 tttgaacgta ctgaaaactt cattagctgc tgaacaagca actgctgaag acaagaaagc
    47821 tgacttgaac cgtcaaaaag cagaagctga agcagagcaa gctcgtattc gtgaacaaca
    47881 acgtctagca gaacaagctc gtcaacaagc tgcacaagaa aaagctgaaa aagaagcacg
    47941 cgaacaagca gaggctgaag cacaagctac tcaagcttca tctactgcac aaagctcagc
    48001 aacagaagaa tcatcagcta cacaatcttc aatgactgaa gaatcatcat cagctacaca
    48061 atcttcagca acagaagaat caacaacacc tgaatcttca acagaagaat caacagcgcc
    48121 tgaatcttca gcaacagaag aatcaacaac agcgcctgaa tcttcagcaa cagaagaatc
    48181 aacaacagtg cctgaatctt cagcaacaga agaatcaaca acagtgcctg aatcttcaac
    48241 aacagaagaa tcaacaacac ctgcgcctac aacaccatca acagatcaaa gtgttgatac
    48301 aggaaacggc acaggaagta gtactccggc tccaacgcca acaccaacac ctgaacaacc
    48361 aaaacctgta acacctgctc cagctccttc tggaagtgtg aatggtgcag caatcgtagc
    48421 agaagcttac aaatatattg gtactccata tgtttggggc ggtaaagatc caagtggctt
    48481 tgactgctca gggttcacac gctatgttta catgcaagta actggtcgtg acatcggtgg
    48541 ttggacagtt cctcaagaat cagctggaac aaaaatttca gtttctcaag caaaagctgg
    48601 agacttgtta ttctgggggt cacaaggcgg aacttaccac gtagcgattg ctttaggcgg
    48661 aggacaatat atccatgctc ctcaaccagg cgaaagcgta aaagttggtt cagttcaatg
    48721 gtttgcccct gactttgctg tcagcatgta agaatgatta atcaaaaaga gttagcccat
    48781 tctggactaa ctcttttttt attgtgataa aatagaaatc agataaattc ttctgtcaat
    48841 tccatgaacg tttggatatc ttcagtcacc atgtcgatac ctgcttgcca aaaatctggt
    48901 tgggtaagat cgacattcaa atgtttcttc gcaagatctt ccgtcgtcat tgaggcagta
    48961 tcgcgtagta aatggatata ttcttcttca aaatttgctc ccttttggtt ggcgtaagca
    49021 tagatgccca aactaaagag gtagccaaat gtgtatggga agttataaaa cggtacgtca
    49081 tcaataaaga aatgcaattt tgcagcccag aaatatggat ggtaagtagc taaagctcct
    49141 tgatagcctt ctttttgcgc ctcaaccatc atctctgtga tcttatcttc tgagactaat
    49201 ccttcttgcc gtgcttcata gaatcgattt tcaaaaatga atcgggaatg gatattcatg
    49261 aacatagcta aagcattttg cagttttgta tctagcaaat tgattttttc ttcttttgtt
    49321 tttgcgactt ttaatgtagc atcggctacg atcagttctg cgaatgtact tgctgtttct
    49381 gcgacattca tggcatattc gcgatttaaa gaaggtaaat cccacatcgt gctgctatgg
    49441 aaagcatgtc ctagttcatg agcaagtgtc gcaacctcat taacagaatt actgtaggtc
    49501 ataaagatcc ttgattcttt tgtttctggt aactcagtac aataaccacc aggacgtttc
    49561 ccaggacgat cttctgcttc gatccagctc ttttcaaaag cagattgggc aaatttagcc
    49621 atttttggac tgaatttgtt aaagttttcg ataataaatg ctgctgcttc gtcaaaacta
    49681 aaggtttttt ctttcaaatc gcctaaaata ataggtgcat cttgatcttg ccattccatt
    49741 ttttctttac cgaaaagatt agcttttcta gtcaagtaat taacaagggg ttgtttgttt
    49801 ttctggattg tatcccacat gacagacaat gtttctttct tcaaccgatt gtactctaat
    49861 ggtttttgta gaaaatctgt gacaccatga agttcatagt cagaaagtct aaaaccatct
    49921 aaatgattca aagtatccgt gaagaggggt gccttttctt tccaggcttc ttcccatgct
    49981 gcgaaaagtg tttctctgac ttttggatct ggatcgccca tcatcttatt gaaggcttgt
    50041 ccagcagata attcgactac ttgtccatct tgttcgaagg gaatcgaaat gctggctaca
    50101 atcgtatcat aatgactgct ccaagcattt agaccatcta aagaaagcgt atttataatg
    50161 ttttcttcag cttctgataa taattgtgag ccatcacgac gaatctcgtt taaacgaaaa
    50221 gcaattgttt caaacgaaga ttgagaaagc atctgattcc aatcattgtc agatatttct
    50281 gcaaatttct ttgacaatac agtttcggct gattgcagac gaggcagcat tgcgtaaagc
    50341 ttgcctgata agacctttgc atctgaatca ttgacattgg cagaaagcaa tgcagtaata
    50401 tagctgctgc attgggtaaa accattcgta atcgtttctt gcaattgaag aatagcgttt
    50461 aactgctctg cattgtcaga actaggtgac cattttgtta cgcggtgata atactcgttc
    50521 atttggtctt ccaattgctt catccgttgg ttcaaagcat cagaatgact gcctccggaa
    50581 aaaatagaat ctagattcca atttaatgaa taactcatga aaaaactccc ttcatattgt
    50641 aatacaaaat taagtatagc acttttttat ggaagaagtc tctatgaaaa tgattgttgt
    50701 ttcttcaaaa aaagtctaag ataaaataca gaggtgactt atgaattctt caaaaaaaca
    50761 aatgaaaaac ggaaatattt atttagtaat cgcactgata gtcgttataa tcttatttta
    50821 cagttcttct caaacttacg gacagcaatc acaagtttcg cgattagaaa gctggctgcc
    50881 taatcagcca tttaaagaca cattagcggg tatacgtttt aattatggtg gaagtatggt
    50941 gagtatcaac catctgggct acgccaaatt tttagagttc tttttaagga agggcgcaca
    51001 tttcggtacc tattttattt tgggaggcag cttgttttta ggtgttttcc ccaagctaaa
    51061 aatttggtgg ctgacagcga tccttgcttg gctttcagca acagggtacg caggtttgga
    51121 cgaattccat cagatgatga ccggtggacg aacacctatg ttccaagatg tcatgttgga
    51181 ttcgatcggt gctctgactg ctgtgatatt atgtgtgctt tttgcggcat tcaagaagcg
    51241 tttatagatt ggctgtggct ttgttttttt gtgaaatagt actagaatag aagtgtattc
    51301 tcaaaagaac aagggaggaa aaatcatgtc aggacatagt aaatggcata atatccaagg
    51361 aagaaaaaat gcccaagatg caaaacgagg gaaagtcttt cagaaacttt ctcgtgaaat
    51421 ttatatggca gctaaagcag gcggtcctga tccatctatg aatccagctt tgcggcttgt
    51481 tgtggacaaa gcaaaagctg caaatatgcc taacgacaat gtggaacggg cgattaaaaa
    51541 ggctacgagt gccgtagatg aaacgaatta cgatgaaatc acgtatgaag gatatggtcc
    51601 gggtggagta ggaatcttag tccatgcatt gacagataac cgtaatcgga cagcgacaaa
    51661 tgtccgagta gcttttactc gaaacggtgg atcactggga gaaacgggtt cagtcagtta
    51721 cttgtttgac cgcaaaggat atatcgctat tgaacgcgat gggttgactg ttgatgaaga
    51781 gacgatgttt gaggatgtat tagaggcggg ggcagaagat ctgcaaactt ctcctgaagt
    51841 attcgagatt tatactgcac cagaagattt tgctgctgta cgagatgagt tggaaaaaga
    51901 gttcactttg gcgcaagcag aattgacgat gattccacaa acgacgattg atttaaatga
    51961 ggaacaaaaa gagcaattgg aacgtctagt agataagcta gaagacgatg atgatgtttc
    52021 agaagtcttt actgctgctg aaatgtaagt agcacattgt tagataataa aaagaaagca
    52081 cctgtaccaa tcgtacgggt gctttctttt tatatattat ttttgttaga agaccaatac
    52141 aggcgttcct ttttcgacca tcccgaataa ttccttcatt acacttggtg gggtgttgat
    52201 gcagccgtgt gaaccgcggg ttttccataa gtctccaccg tattcaggtt gccagtctga
    52261 atcgtggata ccaacacctg tccagtcaat cggcatccag tagtttaccg gactctcgta
    52321 aggtgttcca tcgtcatttg ttccttttaa agttgcatct tcttctttat tccaaacata
    52381 gaagactcca gcaggtgtag gtgtagttgg ctttccggaa acgatatctg tttcaagggc
    52441 cacttttccg tctttgtaat accacatatg ctggttctcc agatcaactt cgatatacgt
    52501 atcttcgatc aatggatgat cagcggttgt gccaccttgc acaatgggtg aacgagtaaa
    52561 atcttggcct gccagaatcg cttttttcaa tgcttctgtc tcactgtctg tttggatcgt
    52621 ccaactgtaa gtgccaactg ggaccgtaac ttctcctctt tttgtacttt taaattttgt
    52681 gtcattcgta cttgtgttat actttgtgcc taaatctgtt acatattgac gtacttgttc
    52741 tgtatctaaa tcgacttttc catcattgta ggtcagccaa ctcataatat cacttgatgg
    52801 gatctggaaa gtctcaccgt ttatactgta agtagctttt acatttgcga tagcattcat
    52861 ggatgcgagt tgttctttca gtgagctgtc ttcagatgtt acttttggtt tttctttgaa
    52921 ttcagtcaat tcaattgtat ctttaccaga gtttaccgca ctttttaatt gtttgaccgc
    52981 agcatcgacg tcgatcgtat ttccattgac ttcaggcttg ataacgaaag agtcacctgc
    53041 tttttcgatc gttgcatcag tagtaggtgt ccggtctttg ttcaaatcag tcagcttagt
    53101 tgtcaatgtc tgaacggtag tgtctagttt ttggtggttt tgactgacag gatctatttc
    53161 ttgtttttct gccgctgaga cataagtcat tccccaagtc catccgtttt gttcatttag
    53221 gatcggagaa agctccgaag tgaaatcatc tttgtagccg agctcggatt ttggtacttc
    53281 ctgccagact tttccatctt ctttaatagc cagtttttta tcagaataag cttcttttaa
    53341 tttttcattt gctttttcga ctgttagatt gctgacattt attccattta tttctgtatt
    53401 cggcaaaaaa tgctttgtgt agtatgtact gcgataggta taaaagctga tcagcactaa
    53461 gagaacacca agaactgaaa gcaaagcaac atgtttcgat ttttttcgat gcgaagatct
    53521 tgtcatatcg acaactcctt aagtaaaagt ttagggtgga acggtctcat gatagcataa
    53581 tttagaggaa aagacgaagg ttatctctac tttatgaaaa aatttagaaa ccataagggt
    53641 gataaaataa aaaaacggaa aacaccgaat agatgttttc cgtcatgttc aaagattatt
    53701 cagcgtctag tttagcaaaa gcttcatcta gaatttcttt taattgctta gcagaagcag
    53761 ccatacggtc ttgctcgctg tcacttaaag gaatttcgat tactttttgg ataccttgac
    53821 ggttgataac agcaggtgca ccaatgtaga tgtcgttcaa tccatattcg ccattcatga
    53881 atactgataa aggaagaaca gcattttcat catctaagat ggcacgagtg atacgtgcta
    53941 atgctactgc gataccatag aaagtagcgc cttttttctc gataattgtg taagcagcgt
    54001 cacgtacgcc aaagaatagg ttaaccatag cttcttcatc gatttctggg ttgtttttta
    54061 cccattcata gatttgcaag ccggcaacat ttgcatgtga ccaaactggg aattctgaat
    54121 ctccgtgttc acctaagatg taagcatgga cgttacgtgc atcaacgtca actaattccg
    54181 caagtgcttg acggaaacga gctgagtcaa gagaggtacc tgaaccgatt acgcgttctt
    54241 tagggaaacc tgagaatttc caagttgagt aagtcaaaat atctactggg ttagcggcaa
    54301 ctaaaaagat accgttaaat cctgattcaa cgatttgtgt gacaacatcg cggttgattt
    54361 ttaggttttt gtttactaag tctaaacgag tttcacctgg tttttgtggt gcgccagctg
    54421 taataacaac taagtcagca tcatgtgcgt ctgcgtaagt agcagaatag atttttttag
    54481 gtgaagtaaa tgccaaagcg tgagacaagt caattgcgtc tccttcagtt ttagcagtat
    54541 tgatatccac aatacctact tcttgagcga tattttgagt aactagagca aaggcatagc
    54601 tagaacctac ggcgccgtct ccaacaagaa taactttttg atgatctttt ttttctgcag
    54661 ctgcagtcat ttagtatcaa tccttccatt gatttgtttt ttcccatgct tatgctaaca
    54721 tttctcaagc cccatgtcac gctatttggc tataaaatat aacagataac aaggttgtat
    54781 tatatgtaaa agcttacatg tgcgttattt cactattttt atatatcaat tatttcatta
    54841 aactttgaag aaatttttaa tttctttatc atttattaac gattctcttt tagccgaaac
    54901 gccttgtttt gtgttatact atgcattgac aagtgtaaaa aagaaaaaag ctcgatggac
    54961 tggaccgcta attagcagtc cagccttttg tgctgctttt tagaattagg tggaaaacaa
    55021 aaatgaaaat gatcgtcggg ttaggaaacc caggtaccaa ataccaatat acaaaacaca
    55081 atatcggctt tatggtcgtg gataaaattg cacgcgagca tcaagctact ttcaaaaaaa
    55141 atccgttcga agcagaagtt gccgaatttt ttcataatgg agaaaagatc cttttagtca
    55201 aaccacaaac attcatgaat gaatctggtc gtgcagtcgg cccactaatg acttattttg
    55261 ggatttatcc agaggaatta gtcgtaatct acgatgattt ggatctggca gtgggaaaaa
    55321 tccggctccg tcaaaaggga agtgccggcg ggcataacgg tatcaaaagt attatttccc
    55381 atttgaatac gaatgtcttt gatcgaatca aggtgggaat cggcaggcca gaagggaaaa
    55441 aaacagtagt ccagcatgtg cttagtcctt tcagcaaaga aaatcagcct ttgatagaag
    55501 aaagcatgtg tcaatctgtt aaagcagtcg aatatctcat agaagggcat tcgttcgtgg
    55561 atgcgatgaa tcgattcaat tgaggaagga ggaaaaggaa tggatattat tcaattagtc
    55621 ggaaaagatc cgcaagtaaa agaatgggtc agcaatttag aagaaaaaat gcctagacaa
    55681 ctaatcacag gattagcggg ctcagcgaaa acacttctat ttgctcgagc gtttaagaaa
    55741 acaaataaaa acatgattat tctgatgcca aatttgtatt atgcgaatca gttagcagaa
    55801 gatctgcaac atgtgattcc gcaagatcag atccatttat ttccagtgga tgaagtgctt
    55861 tctgctgaaa tggccttttc ttctccagaa gcgcgaggcg agagagttgc aacgcttaac
    55921 gcacttcaag aaaaaaaagc aggtatctat ttgttgccag tcgcatcttt gcacaaacga
    55981 ctgccgaata agaaaacttg gctacaggcg caattgaatt ggcatattgg tgatgagata
    56041 gacgtggaaa atcttcctcg ccaattgact ttgatgggct atgtacgaga agagatggtc
    56101 gcaaaaccgg gagaattcag tattcgagga agcattatcg atatttaccc gttaacgaca
    56161 acctatcctg ttcgtgtaga actatttgat gtagagatag attctatgcg gtattttgaa
    56221 gcagaaacac aacgctcaat cgaaaaaacg gaagaaatct gggtttcccc aacgagtgaa
    56281 caagtctacg gaccagagga tctagaacat ggaatagagc aaatgcaaaa aatgctagaa
    56341 aaacggcttg ctgcgaccac agaagaagca gaccgcgagt ttttgcaaga ttacttcggt
    56401 cagctgactt caagctggca agcaggtgtc ccgacagaag aagcaaaatt ctactcagat
    56461 cttctttacc aagaatctgt gacgatcttg gattatttcc cttcaaatag tttactagtc
    56521 gttgatgatt atcagcgaat catggaaaca aatcgggaaa tcgaaaggga agcggcagaa
    56581 tggcagactc aaaaaatcag cgaattgcgg gtgttttctg agcagacatt ttcagcagat
    56641 atacaaaaaa tcgtgcagaa ggaaaagttc accacaacct ttttctcact cttccaaaaa
    56701 gggatgggaa atcttcgttt ccaagcaatc cataattttc aatatcggac gatgcaacag
    56761 ttcttcggac aaatgccatt gctgaaaaca gaaatggacc gttggcgcaa gcaaaaacag
    56821 actgttgtga tctttattcc gacaaaggac agaatacgca aagcagaaca aatgcttcga
    56881 gatgaagaca tattagtagt agaaacagaa aaaaatgcat tgatcagagg acaggtacaa
    56941 ttagttgaag gagctttgca ggctggtttt gaacttcctc aagaaaaaat agtagctatc
    57001 acagaaaaag aaattttcca aaaaacgacg aaaaaacaaa cgagacgtca aacagtaaca
    57061 aacgcagagc gattgaaaag ctataatgag ttaaaagcag gagattacgt tgtccatgcc
    57121 aatcatggta tcgggaaata cattgggatg gaaacgctag aagtagatgg cgttcaccaa
    57181 gactatatga ctattttgta tcaaaacgac gacaaactgt ttattcctgt tactcaactg
    57241 aatctgatcc aaaaatatgt cgcctctgaa gccaaagcgc ctcgcatcaa taagctaggc
    57301 ggaagtgaat ggacgaaaac caagcgaaaa gtctcttcaa aaatcgagga tatcgctgac
    57361 gatctaatca aattatatgc cgcaagagag tcagaaaaag gatatgcttt tggacctgat
    57421 gacgcctacc aaaaagaatt cgaaaatgcc ttcccctact ctgaaactga tgaccagttg
    57481 agaagtgcag cggagatcaa acgggatatg gaaaaagaaa aaccaatgga tcgactactc
    57541 gtaggtgatg ttggttatgg gaaaacagaa gtagcgcttc gagcggcctt taaagcgata
    57601 aaggaaagca aacaagtagc ctttttagtt ccaacaacga tcttggcaca gcagcactat
    57661 gaaacgatgc tggaaagatt tgaaggtttc cccgtaaata tcgggctatt aagtcgtttc
    57721 agaacaagaa agcaacaaaa agaaaccatc gaacaactgc gtacaggtca agttgatatt
    57781 gttgtaggaa cccaccgaat tttgtcaaaa gatattgaat ttagcgattt gggtctactg
    57841 atcattgacg aagaacaacg ttttggtgta aaacataagg aacgattgaa acagttacgt
    57901 gcacaagtgg atgttttgac tctgactgca acaccgattc cgagaacttt gcatatgtcg
    57961 atgttaggcg tgcgggatct ttcagtaatc gaaacaccgc ctgcgaatcg ttatccgatc
    58021 caaacgtatg ttatggaaaa aaatcctggg gctattcgcg aagcaatcca tcgagaaatg
    58081 ggacgaggtg gacaagtatt ctatttatat aatcgggtag aaacgatcga acaaaaagta
    58141 gaagaaatcc aagaactcgt tccggaagca cggatcggtt atgctcatgg gcaaatgaca
    58201 gaggcgcaat tagaaaatac gctttttgaa tttattgaag gtcaatatga tgttcttgtc
    58261 actacaacca tcattgaaac aggagtagat atcccgaacg caaatacgtt gttcgttgag
    58321 aacgctgatt atatgggatt atccacgtta taccaattgc gagggcgggt aggaagaagc
    58381 agccgtgtcg catatgctta ctttatgtat gagcagcaga agatccttaa cgaagtcagt
    58441 gaaaaacgtc ttcaggcaat caaagatttt acagaactgg gatctggatt caagattgcg
    58501 atgcgggatc tttccattcg cggtgcaggt aatctcttag gtgctcagca gcatggattt
    58561 attgatgcag tcggtttcga tatgtattct caaatgctaa gcgaagctgt ttctcgtaaa
    58621 caaggaaaga atagtcaagt ggagaagacc actgttgaga tcgacctcgg tgtcgatgcg
    58681 tatctacctg aaacatacgt ggcagatcaa cggcagaaaa tcgagatcta taaacgaatt
    58741 cgtgaacttg attcgcaaga aatgctagat gaactagaag atgatctgct cgaccgtttt
    58801 ggagaatatc cagaagaagt agcacatctc ttgacaattg ggcagatcaa aatggatggc
    58861 gatcgagcgc tcctagaaac gataaggaaa caggaggcgc atatagtctt tactctaagt
    58921 aaagttggaa caaaacgcta tagtgtggaa cagttattcg aagcactgac agcgacaaaa
    58981 ctaaaagcca gtctaggcgt tgaaaaagaa cagatggtga tacgcttgac gatccccaac
    59041 caaatggaag aagcagtatg gcttcaggaa atccaaaagt ttgtcaaagc gttgcgtgag
    59101 caaaaatatc tgtcagcgta ggaaggagca atgatttgga tcataaagaa atgcggcgcg
    59161 tcatgcaagg agcatttgta ttgacgattg cttctttcat agcaaaagta ttaagtgccc
    59221 tttaccgaat tcccctccaa aacttagtcg gcgacgaagg tttttatgtt tatcagcaag
    59281 tttatccaat ttatgggata gcaatgactt tagctttatc cggtcttccg cagttcatat
    59341 caaagtacgt tgcagaaaga aagaatcctt tcgagcgaaa gcaagctttg caaaatcttt
    59401 atcccttagt attttggaca ggtgtctttt tgtggttgtt tgtctttgca tttagcagct
    59461 ggattgcttg gatgatggga gacatacaat tgaaaccgct gatccaagta gtttccttta
    59521 cctttttgct gatgcctggt ctatcttttt atcgagggaa tttccaagga agattcttga
    59581 tggaaccaac tgctgtttct caagtagttg agcagttcgt tcgtgttttt gtgattgtct
    59641 gtagtgcgtt tgcttttaga aagcttggat ggacggtgta ccaaacaggg acggcagcaa
    59701 tgagtggagc tgtttttggc ggtttatgcg catatgggat ccttcactac tatgaacaaa
    59761 agattcatgg aggagcgtta agcttccgac ggtttccatt gttcaaacag ccgtcaaagc
    59821 ccttgatccg gcgttttctg gtagaaggcg gactggtttc gatttatagt ggattgctta
    59881 tctttttcca gctgatcgac tctttctttg taaaaaatgc tttggaagtt tacggactgt
    59941 cagaatacgg tgcgaagata gcaaaagggg tctatgatcg aggtcagcct ttagtacaat
    60001 taggtttagt gattgccact gcattaagtg cgacattcct gcctgcgttg actcgtcatt
    60061 tgaccaaccg gatctatcgt cagtttttac agacagccaa aatctatctt agattgacga
    60121 ccgctttagc attagctgcg tctttgggat tagccttgct gctcccttat atcaattatg
    60181 cgttgtttaa agattatgcg ggaaatgcag cgcttgtatt atttgttttt tccattgctt
    60241 tcactgcagt catccaagct tatcaaagta ttgctcaaag caagaactcg tttcgccctt
    60301 ctttaaaagg cgcaggatgg ggactattgg taaaaggatt gactacttct tttctgaccg
    60361 gtttattagg aacagcaggt gcaagcctgt ctaccctttt aggattaggt gtgacgcttt
    60421 ggtattttgt tcgaattgaa acaaaagagc taaatgcttt ttggaaagaa cggcattttg
    60481 gacggaagtt gatgttcagc cttctttgta tgacagtagt actgctgctg tattatggtg
    60541 tattgaatat agtatttggt cctgtaactc atcgttccac tgcttttttt gtcagcgtga
    60601 taggagttgg gatcggggct tctgtgttca tttatgcagt gattcgtctt cgtctgttta
    60661 cagtacgaga atggctaatg ttgccttttg gtaagaaaat cctgcgatta aataaaaaaa
    60721 ttaaaaagtg aggaataaga tatgcgatta gataaatttt taaaaatttc ccgaatcatc
    60781 aaaagacgat cagtagcaaa agaagtagct gataaaggaa gaattcaaat caacggtaaa
    60841 ttggcgaaat cttccagtga tgtcaaagta ggcgatattt tgaaagtcca gtttggcaac
    60901 aaaacgttgg aagtgaaaat tacggaatta catgaatcaa ccaaaaaaga agatgcacaa
    60961 aaaatgtacg aaattatttc tgaaaatcgc acagaaacac tagagaaata gattagacaa
    61021 gctttcacga tattggtata atgaaaaaga caatcaaaaa gattggagcg aatccaagtg
    61081 acgaaaaaaa gcaataaaat cgctgcgcta gatacggaat atgcaaagaa aaagtatgct
    61141 gaatttcagc aacaacaacg tcaactgatt tttcgaagac gcagactggc tgcaattttt
    61201 gttggtgctt tggtcatttt cgcgataata ggtgtgcaga ttttccatga catgcaaagg
    61261 atgcatcaat tgaacgaatt gagagtcgaa gcaaatactg aaatgaaaga agtcaacgca
    61321 gacgttgatc agctgcacca agatgtttca ttgctgaaag atgatgagta tgtagctaaa
    61381 cttgccagaa gcaaattctt ttactcaaaa gacggtgaaa aagtttatcc tatattagaa
    61441 tcaaatacag acagctcgac aaatgaaaca gatcaatcaa ctagttctac aactgcaaaa
    61501 taagcaggaa tataaaaaag ggaaataatt aggaggaaca ttttttttat gtcaatcgag
    61561 gtaggagcaa aactgcaagg aaaagtatca ggaatcacaa attttggtgc gtttattgat
    61621 ctaggtgctg gaaaaacggg tctagttcat attagcgaag tgtcaaatgg ctatgtaaaa
    61681 gacatccatg atgttttaag tgtaggagat gaagtaacag ttaaagttat ttcagtaaat
    61741 gatgatggaa aaatcggact atctatccgg aaagcgcagg aacaacctgc tgaatctgga
    61801 gaagaaagcc gtcctaaaaa atatcaacaa aaagatcccc gtgacaaccg aaatcgtcca
    61861 gcacagaaaa aatctttttc tcgcagtcaa ccttcagctc aaaaagaaga cttcgattca
    61921 ttgatgagtt catttttgaa agatagcgat gaccgcttat cttcattgaa gcgaaacaca
    61981 gaaggaaagc gcggtggccg tggcggacgt cgcagctaaa aagccgcaaa taatgatagt
    62041 gcgactattt gggtcatagt atcaacagag ggcaggttaa ttgtcgtttt agacgttttc
    62101 ccgctctctt ttttttagga agaaagggtg tggaaaggat gaacgttgaa aaaggcggga
    62161 aaaaagcacc gctattacag cagtcattta ttcggcacgg gaatgaacaa ggattctggc
    62221 aacaaggatc gagaatttta gcagcggtat caggtggtgt tgattcgatg gttttgctcc
    62281 atttactttt acaagtgcaa cgccaaatcg gtttttcttt aggtatcgtc cacatcaatc
    62341 accaattaag agaagaatca gcagaagaag ctgcctatct tcgctcatat tgtgaagaga
    62401 agaatctccc tttatatgta agtatttggg aagatccagc caaatccgcc atagaaacag
    62461 aagctcgtaa ctttcgttat caagaattca ctcgcgtgat ggaagaagga caatatgaca
    62521 cgttgatgac tgctcatcac ggagatgatc aaatcgagac ggtgttaatg aaaatcgttc
    62581 gtgggggaca gttaaatact ttttctggaa tcaaagaagt acaaccattt gctacgggca
    62641 gactgattcg tccgttgctg agttttagca aagaagacct atatacttat gcagcggaat
    62701 cgcagcttgt ttactttgag gatcagacaa accaattgtt agatatccag agaaatcggc
    62761 ttcgtcattt ggtagttcct caattgaaac aagaaaatac acaggcgttg agacatttcc
    62821 agcaattttc gcaacagatc caatgggcag accaagtcat tcaaaaatat atgggacagt
    62881 tgatcgaaaa ggaagtcgaa cagtgggagg aacaatttcg attttctgca gaaatgattg
    62941 aagaaatgac agatgctgaa cgatactatt ttttttactc ctttttcgac aaagcattct
    63001 caaaaacagg aattattttg aaagaacaac aaatggagtc gattttagac cagtggcaac
    63061 agaaaaaaag ccaatggcaa ctcagtattg gcaataattg gatactgaaa agagagtatc
    63121 attttgttgt actctctaaa aaagaaaaac aagcaactag ccagacagaa gaccagcgct
    63181 tgttgctgat tccagaacaa ggaatatatt tgaatgaaac agaatggatt ggtctggtca
    63241 aacctgaaaa tgcaggggac atcaaagcaa cagattcatg gtctctattt tctcatgaca
    63301 tttggattcc tgctgatgtg caactgtatg tcgaaaaaag aaaagcagga gaccgcatta
    63361 aattaacaga gaatctcaca aaaaaagtat ctagatattt tatcgatcag aaagtaccta
    63421 attcacaaag gcagcatgct tggatcatta cagatgccaa aagaaatata tatagcttga
    63481 taccttttgc cttttcatat ttgagtattc gtgaagaaac tgataaaata cactacatac
    63541 ttctttacaa atatcaaaaa gaagcaattg gaaggagaac ctgatgttgg aaaaggatat
    63601 tgagagaatt ttgatctcac aagaagagat acaagttcgt tgcaaagaac taggcaaaga
    63661 gttaacagaa atttaccaaa acaccaatcc cctagtagta ggtgtcttaa aaggagcggt
    63721 accattcatg gcagatatcg ttcgttctat cgatacgtat ttggaattgg atttcatgga
    63781 tgtttcaagc tacgggaatg caacagtctc gtcaggggaa gtaaaaatcg tcaaagactt
    63841 agatacgaat gtagaaggtc gcgatctgtt gatcgttgaa gatatcattg acagcggaag
    63901 aacacttgct tatttagtgg atcttttcaa ataccgcaaa gcaaaatcag tcaaaattgt
    63961 tacactactt gacaaacctg aaggaagagt agtaaatatc gaagcagatt atgttggctt
    64021 caacgtacca aatgaatttg tggtcggtta tggcttggat tatgcagaag catatcgtaa
    64081 ccttccgtac atcggtgtat tgaaacctag tgtttatcaa tcaaattaat gctgtatctc
    64141 aattaaatta tggtacaata gaatggtcag gattgataaa aaactgttaa attaaattga
    64201 cgcttaggga gggcaactat gaacaaaaag aacaacggca tgatgaaaaa tgccttgtat
    64261 tatgtcctcg taatcttagc gatggtaatg gtcgtttatt tcatttttgg taataacggc
    64321 cagcgatcgt cagatatcga gtactcaaaa ttcacagaac aactacaagc aggcaagatc
    64381 aaagaatttg aagtgcagcc tgcaaatggt gtatataaga tcactggtga atacaaagaa
    64441 cagcagaccg tcgctaacag cggtgggtta gctatcttag gttcaacaga tacgactact
    64501 acccatttca caacaatcat tttgccaaat gatacaacat tgagccaagt aacagaactg
    64561 gcaaaagaaa acaaagttgc tacaacagta aaagaagaat caagcagcgg tatatgggtt
    64621 tccctattgc ttagcttctt gccaatcgta atcatcatct ttttctttta catgatgatg
    64681 ggacaacaag gcggcggcgg tggcggtggc ggccgtgtca tgaactttgg taaatcaaaa
    64741 gccaaagaag cagacaaaaa agctaatcgc gtacgttttt ctgatgtagc gggtgcggaa
    64801 gaagaaaaac aagaactagt tgaagttgtt gaatttctga aagatcctcg ccgatttgtt
    64861 gaattaggtg cccgtatccc ggcaggtgta cttttagaag gacctccagg aacaggtaaa
    64921 accttattag caaaagctgt tgccggagaa gctggagtac cgttttactc gatctcgggt
    64981 tctgatttcg tagaaatgtt tgtcggtgtt ggagcaagtc gtgttcgtga tttgtttgaa
    65041 acagcgaaga aaaacgcacc agctatcatt ttcattgatg aaattgatgc ggtaggtcgt
    65101 caacgtggtg ccggtatggg cggcggacat gatgaacgcg aacaaacatt gaaccaattg
    65161 cttgtagaaa tggatggctt cgacggaaat gaaggagtaa tcgtcattgc tgcaaccaac
    65221 cgttctgatg tactagaccc agcgttacta cgtccaggcc gttttgaccg ccagatttta
    65281 gttggacgtc cagatgtaaa aggtcgtgaa gcaatcttgc gagtccatgc acggaataaa
    65341 ccgatggcag atgacgttga cttgaaagtt gtggctcaac agacaccagg ttttgccgga
    65401 gcagatttag aaaatgtctt gagtgaagca gcgttagtag ctgctagaag aaataaaaag
    65461 aaaatcgatg cttctgatat cgatgaagcg gaagatcgcg tgattgcagg tccagctaaa
    65521 aaagatcgcg taatcagcaa aaaagaacgc gaaatggttg cttatcacga agctggtcat
    65581 acgattgtcg gattagtatt gagtcgcgct cgggtcgtcc acaaagtaac gatcatccct
    65641 cgcggacgcg ccggcggata tatgatcgct ttaccaaaag aagatcagtt cttgatgacg
    65701 aaagaagata tgtttgaaca aatcgtaggc ttacttggtg gacgtacagc tgaagaaatc
    65761 atcttcaatg tccaatcaac tggtgcttcc aatgactttg agcaagcgac agctctagca
    65821 cgaagcatgg taacagaata tggaatgagt gatcgtttag gtcctgttca atatgaagga
    65881 aaccatcaag tgtttgttgg ccgcgactat ggtcaaacaa aagcttactc ggaacaagtc
    65941 gctttcgaga ttgatcaaga agtacgaaaa atcttgatgg aagctcatga taaagctcga
    66001 gaaatcatcg aagctcatcg tgcacagcat aaactgattg ctgaaaaact tttggaattc
    66061 gaaacattgg atgccaaagc aatcaagtca ttgtttgaac acggtgtgat gcctgaaggt
    66121 actgagtcaa atgattatcc aagcgaaaaa gaagcgaaaa cttttgaaga agcaaaacgt
    66181 gctttggaag aaaaagacgc tgaaaaacaa gcggaagaaa aacgtgattt tgaagaagca
    66241 aaacatgaac tgaatgagga aaataaagca gaacatgatt cagattctca acaatcagaa
    66301 gataatcatt cagacgatca atacaaataa aaaaatatag gtcaattgat ctgatatgac
    66361 tcccgaaaac cggaacaaaa accggttttc gggagatttc tttatttaag ttgtaaatag
    66421 ggtgatgtcc tgttttctag atgattctga attattttta aaagaagcat tccaaagttg
    66481 aaaaaagcag tagttgctag tatgattcac tatgagttta aaaacggaaa aaggagtaca
    66541 taacaaatga aagattattt ggttaaggca cttgctcacg acggattcgt ccgagcatac
    66601 gccgttcaag caacaaatac cgtagcggag gcacaaagac gtcatgatac ttggcacacc
    66661 tcctcagctg ctttgggacg gacattggtc ggtagtctgc tacttggtgc gacgctcaaa
    66721 ggagaagaca aactaactgt tcgaatccaa ggcgatggtc cagcaacagg aattgtagta
    66781 gatgcaaatg gaaagggaga tgtgaaaggt tacatcaaaa atccgcatct cagtcttcct
    66841 ctaaatagtg aaggtaaaat cgatgtgcgg ggagcagttg gaacacaagg aatgtttact
    66901 gtcacaaaag acttaggatt aaaagaacct ttttctgggc aaacgccgat tgtttctgga
    66961 gaaatcgctg aagattttac ttactatctt gctatctcag aacaaatccc ttcagctgtt
    67021 ggactgagtg tattggtcaa tactgacgac agcatcaaga cggcgggagg attcatgatc
    67081 caaatcatgc caggtgccga tgaagaaaca atcagcaaga tcgaagaaca attaaaaacg
    67141 acaccaagaa tctctaccct cttagatgaa ggacagacac cagaagaaat attgaaagga
    67201 ttgcttggta cagaagaatt gaacattttg gaaaaaatgc ctgttcaatt caaatgtgac
    67261 tgttcaaaag aaaaatttgc agaagcaatt atcgcgttag gtgcagaaga aatccaagca
    67321 atgatagacg aagatcacgg tgcagaagcg gtttgttctt tctgtaataa caaatacaag
    67381 tatagcgaag aagaattaga tggattgaag aaagaagcct taggagggat ttaagtggaa
    67441 cacacatgga aaattggcaa tgtggaaatc cctaatcgtg tcgttgttgc accgatggct
    67501 ggaataacaa attcagcatt tcgagtcact gtcaaagaat ttggtgcggg cttagtcgta
    67561 tgtgaaatga tcagtgacca aggtattcat ttcagaaaca aaaaaacttt agagatgctc
    67621 cacattgaag aaacagaaca tccattaagt ttacagatat ttggcggaaa taaagatagt
    67681 ctagttgaag ctgctcaatt tgtcgaagaa aacacagctg ccgatataat agatatcaat
    67741 atgggctgtc cagtaaataa agtaatcaaa gcagaagcag gagcaaaatg gcttttagat
    67801 cctgagaaag tatacgaaat ggtccaagct gtttcttcag cagtaaatat cccagtaaca
    67861 atcaagatgc gtattggttg ggatgaagag cacgtctttg ctgtagaaaa tgcattagca
    67921 gcacaatcag ctggagcaag tgccatcgct atgcatggac gtacacgggt tcagatgtat
    67981 gaaggaaaag cggactggga agtattgaaa gaagtgaaaa aacatttgac gattccattt
    68041 atggggaacg gggatgtcaa aacgccagaa gatgcaaaat ggatgcttga ctatgtcggt
    68101 gcagacggtg tgatgattgg tcgtgcagcg ctgggcaatc catggatgat tcaccgcaca
    68161 cagcattatc tcgaaacagg ggaactgatt cctgaaccaa caccagccga aaaaattgct
    68221 acagctaaac ttcatttaca acgcttagca gatctcaaag gagaaaaagt agcttgtaga
    68281 gaattcagaa cacatgcggc ttattattta aaagggattc ctcgagcagc gaaagtgaaa
    68341 gtagcaatca atcagacaga aaaacagcaa gaaatcaatg acctgctgga tcaatttgta
    68401 gaaaaagcgg aaaaagtact attgaaacaa acgttgtgat ttctaagttt ttcgtgaatt
    68461 ttcaagccga aattccaagt tcggcttgct ttttttgtgt ttcattggca ttataggaaa
    68521 gagattaaca tgagaatgga ggaacttttg tgacacagga acaacaacat caagtagaag
    68581 atttaaatga tcaaatgctt gtacgtcgtc aaaaaatgga acaattgcgt gaagatggaa
    68641 tcgatccatt tggaaaacga tttgaacgca cgcacaactc agaagaattg catgaactat
    68701 ttgatccaag aacaaaagaa gaactagcag aaatgggatt gaccgctagt gtagctggtc
    68761 gtatgatgac tcgccgagga aaagggaaag ctggatttgc gcatctacaa gatcgtgaag
    68821 gccaaattca aatttatgta agaaaagacc aagtaggtga tgaggcctat gaagtattca
    68881 aacatgcaga ccttggagat ttctttggtg taactggaca aatcatgaaa acagacacag
    68941 gcgaagtttc tatcaaagct tcagaaatca cgattttgtc aaaagcactt cgtccactgc
    69001 cagataaata ccatggctta acgaatattg aacaacgcta cagacaacgc tacttggatt
    69061 taatcagtaa tcgcgaaagc tttgaccgat ttatgaaacg aagccaaatt atcagcgaaa
    69121 tccgtcgcta tttagatggc aatggctata ttgaagtaga aacaccagta ttgcacaatg
    69181 aggcaggtgg agctgccgca cgcccattta tcacgcatca taatgcactg gacattgatt
    69241 tatatttgcg tattgcgcta gaacttcatt taaaacgact aattgttggc ggaatggaaa
    69301 aagtttatga aatcggccgc gttttccgta atgaaggaat tgatactaca cataatccag
    69361 aatttacgat gttagaagca tacacagcat atactgattt caatgatgta atgaatctga
    69421 cagaaggtat tatccgtaac gcagctgaaa aagtactagg aacagcaaaa atcacatatg
    69481 atggacaagc tgtcgactta gaaagtgact ttaaacgcat ccatatggta gatgcaatca
    69541 aagaacaaac tggtgtagac ttctggcaag aaatgacatt ggaagaagca ttagctttag
    69601 ccgaaaaaca caatgtagaa gtaaccgaag ctatgggtgt cggacatgtt atcaatgaat
    69661 tctttgaaac atttgttgaa gaaacattga cacaaccgac gtttgtatac ggacatccag
    69721 tcgaagtttc gcctttagcg aagaaaaatc cagaagatcc tcgattcaca gatcgattcg
    69781 aactattcat agtggggcgc gaatttgcta atgcctttac agaactgaac gatccaattg
    69841 atcaaagaga aagattcgaa gcgcaagaaa aagaacgtga actaggaaat gacgaagctc
    69901 acggtgtaga cgaagacttc ttggaagcct tagaatacgg tatgccgcca actggtggtc
    69961 taggcatcgg gatcgacaga ttagtaatgt tgctaactga tgcacaatca attcgggatg
    70021 ttttgctatt cccaacaatg agataatcga ataaaagctg tctaaggaat t
//
LOCUS       DADOZM010000003        60876 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.003, whole genome shotgun sequence.
ACCESSION   DADOZM010000003 DADOZM010000000
VERSION     DADOZM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.003"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="J8I47_000140"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="J8I47_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAZ6321609.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="J8I47_000141"
     CDS             join(2859..2921,2923..3960)
                     /gene="prfB"
                     /locus_tag="J8I47_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAZ6321610.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="J8I47_000142"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="J8I47_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAZ6321611.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="J8I47_000143"
     CDS             4735..5628
                     /locus_tag="J8I47_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321612.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="J8I47_000144"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="J8I47_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAZ6321613.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="J8I47_000145"
     CDS             7565..8422
                     /locus_tag="J8I47_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAZ6321614.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="J8I47_000146"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="J8I47_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAZ6321615.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="J8I47_000147"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="J8I47_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAZ6321616.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="J8I47_000148"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="J8I47_000148"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321617.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="J8I47_000149"
     CDS             11178..11936
                     /locus_tag="J8I47_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321618.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="J8I47_000150"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="J8I47_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAZ6321619.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="J8I47_000151"
     CDS             12864..13895
                     /locus_tag="J8I47_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAZ6321620.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="J8I47_000152"
     CDS             13892..14659
                     /locus_tag="J8I47_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAZ6321621.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="J8I47_000153"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="J8I47_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAZ6321622.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16627..16944
                     /locus_tag="J8I47_000154"
     CDS             16627..16944
                     /locus_tag="J8I47_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAZ6321623.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16944..17294
                     /locus_tag="J8I47_000155"
     CDS             16944..17294
                     /locus_tag="J8I47_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAZ6321624.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="J8I47_000156"
     CDS             17461..18399
                     /locus_tag="J8I47_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAZ6321625.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="J8I47_000157"
     CDS             18414..19244
                     /locus_tag="J8I47_000157"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAZ6321626.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="J8I47_000158"
     CDS             19288..20313
                     /locus_tag="J8I47_000158"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAZ6321627.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="J8I47_000159"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="J8I47_000159"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAZ6321628.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="J8I47_000160"
     CDS             21391..21831
                     /locus_tag="J8I47_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAZ6321629.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="J8I47_000161"
     CDS             21834..22436
                     /locus_tag="J8I47_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAZ6321630.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="J8I47_000162"
     CDS             complement(22495..23601)
                     /locus_tag="J8I47_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAZ6321631.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="J8I47_000163"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="J8I47_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAZ6321632.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="J8I47_000164"
     CDS             complement(25149..27494)
                     /locus_tag="J8I47_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAZ6321633.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="J8I47_000165"
     CDS             27773..28675
                     /locus_tag="J8I47_000165"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAZ6321634.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="J8I47_000166"
     CDS             28888..30663
                     /locus_tag="J8I47_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAZ6321635.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="J8I47_000167"
     CDS             complement(30729..32315)
                     /locus_tag="J8I47_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAZ6321636.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="J8I47_000168"
     CDS             32566..32790
                     /locus_tag="J8I47_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAZ6321637.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="J8I47_000169"
     CDS             32932..34683
                     /locus_tag="J8I47_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6321638.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="J8I47_000170"
     CDS             34683..36467
                     /locus_tag="J8I47_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321639.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="J8I47_000171"
     CDS             36793..37644
                     /locus_tag="J8I47_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6321640.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="J8I47_000172"
     CDS             37698..39449
                     /locus_tag="J8I47_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6321641.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="J8I47_000173"
     CDS             39453..39623
                     /locus_tag="J8I47_000173"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321642.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="J8I47_000174"
     CDS             complement(39663..40565)
                     /locus_tag="J8I47_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6321643.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="J8I47_000175"
     CDS             40796..42199
                     /locus_tag="J8I47_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ6321644.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /gene="ascB"
                     /locus_tag="J8I47_000176"
     CDS             42199..43632
                     /gene="ascB"
                     /locus_tag="J8I47_000176"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ6321645.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="J8I47_000177"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="J8I47_000177"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAZ6321646.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="J8I47_000178"
     CDS             44917..46644
                     /locus_tag="J8I47_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAZ6321647.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46716..47009
                     /locus_tag="J8I47_000179"
     CDS             46716..47009
                     /locus_tag="J8I47_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAZ6321648.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="J8I47_000180"
     CDS             complement(47101..47961)
                     /locus_tag="J8I47_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAZ6321649.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="J8I47_000181"
     CDS             48053..48481
                     /locus_tag="J8I47_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ6321650.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="J8I47_000182"
     CDS             48643..48819
                     /locus_tag="J8I47_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAZ6321651.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="J8I47_000183"
     CDS             48846..49292
                     /locus_tag="J8I47_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAZ6321652.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="J8I47_000184"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="J8I47_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAZ6321653.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="J8I47_000185"
     CDS             51531..52502
                     /locus_tag="J8I47_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAZ6321654.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="J8I47_000186"
     CDS             52526..54718
                     /locus_tag="J8I47_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAZ6321655.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="J8I47_000187"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="J8I47_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAZ6321656.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55189..55590
                     /locus_tag="J8I47_000188"
     CDS             55189..55590
                     /locus_tag="J8I47_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAZ6321657.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /locus_tag="J8I47_000189"
     CDS             55605..56504
                     /locus_tag="J8I47_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAZ6321658.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="J8I47_000190"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="J8I47_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAZ6321659.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="J8I47_000191"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="J8I47_000191"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAZ6321660.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="J8I47_000192"
     CDS             58762..60837
                     /locus_tag="J8I47_000192"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAZ6321661.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19864 a        10760 c        13026 g        17226 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaaggac atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaat
//
LOCUS       DADOZM010000004        60192 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.004, whole genome shotgun sequence.
ACCESSION   DADOZM010000004 DADOZM010000000
VERSION     DADOZM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.004"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="J8I47_000193"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="J8I47_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAZ6321662.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="J8I47_000194"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="J8I47_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAZ6321663.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="J8I47_000195"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="J8I47_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAZ6321664.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="J8I47_000196"
     CDS             1642..2274
                     /locus_tag="J8I47_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAZ6321665.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="J8I47_000197"
     CDS             complement(2276..3181)
                     /locus_tag="J8I47_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ6321666.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="J8I47_000198"
     CDS             complement(3266..3919)
                     /locus_tag="J8I47_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ6321667.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="J8I47_000199"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="J8I47_000199"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAZ6321668.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="J8I47_000200"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="J8I47_000200"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAZ6321669.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="J8I47_000201"
     CDS             6168..7253
                     /locus_tag="J8I47_000201"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAZ6321670.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="J8I47_000202"
     CDS             7250..8314
                     /locus_tag="J8I47_000202"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAZ6321671.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="J8I47_000203"
     CDS             complement(8563..9366)
                     /locus_tag="J8I47_000203"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAZ6321672.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="J8I47_000204"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="J8I47_000204"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAZ6321673.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="J8I47_000205"
     CDS             complement(10244..11251)
                     /locus_tag="J8I47_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAZ6321674.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="J8I47_000206"
     CDS             complement(11258..11902)
                     /locus_tag="J8I47_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAZ6321675.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="J8I47_000207"
     CDS             complement(12002..12760)
                     /locus_tag="J8I47_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAZ6321676.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="J8I47_000208"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="J8I47_000208"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAZ6321677.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="J8I47_000209"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="J8I47_000209"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAZ6321678.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="J8I47_000210"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="J8I47_000210"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAZ6321679.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="J8I47_000211"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="J8I47_000211"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAZ6321680.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="J8I47_000212"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="J8I47_000212"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAZ6321681.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="J8I47_000213"
     CDS             18158..19027
                     /locus_tag="J8I47_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ6321682.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="J8I47_000214"
     CDS             complement(19153..20052)
                     /locus_tag="J8I47_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ6321683.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="J8I47_000215"
     CDS             complement(20217..21041)
                     /locus_tag="J8I47_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ6321684.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="J8I47_000216"
     CDS             complement(21052..22347)
                     /locus_tag="J8I47_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAZ6321685.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="J8I47_000217"
     CDS             complement(22363..22842)
                     /locus_tag="J8I47_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAZ6321686.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="J8I47_000218"
     CDS             complement(22848..23876)
                     /locus_tag="J8I47_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAZ6321687.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="J8I47_000219"
     CDS             complement(23893..24999)
                     /locus_tag="J8I47_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAZ6321688.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="J8I47_000220"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="J8I47_000220"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAZ6321689.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="J8I47_000221"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="J8I47_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAZ6321690.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="J8I47_000222"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="J8I47_000222"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAZ6321691.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="J8I47_000223"
     CDS             29970..30668
                     /locus_tag="J8I47_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAZ6321692.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="J8I47_000224"
     CDS             complement(30836..33475)
                     /locus_tag="J8I47_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ6321693.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="J8I47_000225"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="J8I47_000225"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAZ6321694.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="J8I47_000226"
     CDS             35318..36493
                     /locus_tag="J8I47_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ6321695.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="J8I47_000227"
     CDS             36497..37138
                     /locus_tag="J8I47_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321696.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="J8I47_000228"
     CDS             37135..38052
                     /locus_tag="J8I47_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6321697.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="J8I47_000229"
     CDS             38049..38720
                     /locus_tag="J8I47_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321698.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="J8I47_000230"
     CDS             complement(39261..39749)
                     /locus_tag="J8I47_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAZ6321699.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="J8I47_000231"
     CDS             complement(39950..40216)
                     /locus_tag="J8I47_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ6321700.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="J8I47_000232"
     CDS             complement(40216..40479)
                     /locus_tag="J8I47_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ6321701.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="J8I47_000233"
     CDS             complement(40472..40744)
                     /locus_tag="J8I47_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321702.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="J8I47_000234"
     CDS             40955..41140
                     /locus_tag="J8I47_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6321703.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="J8I47_000235"
     CDS             complement(41282..43105)
                     /locus_tag="J8I47_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAZ6321704.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="J8I47_000236"
     CDS             complement(43703..46408)
                     /locus_tag="J8I47_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAZ6321705.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="J8I47_000237"
     CDS             complement(46714..47661)
                     /locus_tag="J8I47_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAZ6321706.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="J8I47_000238"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="J8I47_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAZ6321707.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="J8I47_000239"
     CDS             complement(48296..48658)
                     /locus_tag="J8I47_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ6321708.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="J8I47_000240"
     CDS             complement(48669..49790)
                     /locus_tag="J8I47_000240"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAZ6321709.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="J8I47_000241"
     CDS             complement(49806..50156)
                     /locus_tag="J8I47_000241"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAZ6321710.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="J8I47_000242"
     CDS             complement(50286..51797)
                     /locus_tag="J8I47_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ6321711.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="J8I47_000243"
     CDS             complement(52119..53471)
                     /locus_tag="J8I47_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAZ6321712.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="J8I47_000244"
     CDS             complement(53624..54700)
                     /locus_tag="J8I47_000244"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAZ6321713.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="J8I47_000245"
     CDS             54896..56641
                     /locus_tag="J8I47_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAZ6321714.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="J8I47_000246"
     CDS             complement(56921..57358)
                     /locus_tag="J8I47_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAZ6321715.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="J8I47_000247"
     CDS             complement(57342..58715)
                     /locus_tag="J8I47_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321716.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="J8I47_000248"
     CDS             complement(59037..59525)
                     /locus_tag="J8I47_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6321717.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="J8I47_000249"
     CDS             complement(59547..60149)
                     /locus_tag="J8I47_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ6321718.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DADOZM010000005        47429 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.005, whole genome shotgun sequence.
ACCESSION   DADOZM010000005 DADOZM010000000
VERSION     DADOZM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.005"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..1406
                     /gene="rseP"
                     /locus_tag="J8I47_000250"
     CDS             138..1406
                     /gene="rseP"
                     /locus_tag="J8I47_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAZ6321719.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1474..3183
                     /locus_tag="J8I47_000251"
     CDS             1474..3183
                     /locus_tag="J8I47_000251"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAZ6321720.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3395..7753
                     /locus_tag="J8I47_000252"
     CDS             3395..7753
                     /locus_tag="J8I47_000252"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAZ6321721.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7898..8371
                     /gene="rimP"
                     /locus_tag="J8I47_000253"
     CDS             7898..8371
                     /gene="rimP"
                     /locus_tag="J8I47_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAZ6321722.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8394..9569
                     /gene="nusA"
                     /locus_tag="J8I47_000254"
     CDS             8394..9569
                     /gene="nusA"
                     /locus_tag="J8I47_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAZ6321723.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9591..9884
                     /locus_tag="J8I47_000255"
     CDS             9591..9884
                     /locus_tag="J8I47_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAZ6321724.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9881..10192
                     /locus_tag="J8I47_000256"
     CDS             9881..10192
                     /locus_tag="J8I47_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAZ6321725.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10205..12511
                     /gene="infB"
                     /locus_tag="J8I47_000257"
     CDS             10205..12511
                     /gene="infB"
                     /locus_tag="J8I47_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAZ6321726.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12538..12885
                     /gene="rbfA"
                     /locus_tag="J8I47_000258"
     CDS             12538..12885
                     /gene="rbfA"
                     /locus_tag="J8I47_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAZ6321727.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13042..13215)
                     /locus_tag="J8I47_000259"
     CDS             complement(13042..13215)
                     /locus_tag="J8I47_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6321728.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13663..16025
                     /locus_tag="J8I47_000260"
                     /pseudo
     CDS             13663..16025
                     /locus_tag="J8I47_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16393..17316
                     /gene="truB"
                     /locus_tag="J8I47_000261"
     CDS             16393..17316
                     /gene="truB"
                     /locus_tag="J8I47_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAZ6321729.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17320..18261
                     /gene="ribF"
                     /locus_tag="J8I47_000262"
     CDS             17320..18261
                     /gene="ribF"
                     /locus_tag="J8I47_000262"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAZ6321730.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18613..19398
                     /locus_tag="J8I47_000263"
     CDS             18613..19398
                     /locus_tag="J8I47_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6321731.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19411..20262
                     /locus_tag="J8I47_000264"
     CDS             19411..20262
                     /locus_tag="J8I47_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAZ6321732.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20386..20838)
                     /locus_tag="J8I47_000265"
     CDS             complement(20386..20838)
                     /locus_tag="J8I47_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6321733.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(21303..21671)
                     /locus_tag="J8I47_000266"
     CDS             complement(21303..21671)
                     /locus_tag="J8I47_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAZ6321734.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            21821..22303
                     /locus_tag="J8I47_000267"
     CDS             21821..22303
                     /locus_tag="J8I47_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAZ6321735.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            22340..23350
                     /locus_tag="J8I47_000268"
     CDS             22340..23350
                     /locus_tag="J8I47_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAZ6321736.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(23465..24178)
                     /locus_tag="J8I47_000269"
     CDS             complement(23465..24178)
                     /locus_tag="J8I47_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321737.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24368..24958)
                     /locus_tag="J8I47_000270"
     CDS             complement(24368..24958)
                     /locus_tag="J8I47_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAZ6321738.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            25442..25669
                     /locus_tag="J8I47_000271"
     CDS             25442..25669
                     /locus_tag="J8I47_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAZ6321739.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            25685..26851
                     /locus_tag="J8I47_000272"
     CDS             25685..26851
                     /locus_tag="J8I47_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAZ6321740.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            27085..28128
                     /gene="hrcA"
                     /locus_tag="J8I47_000273"
     CDS             27085..28128
                     /gene="hrcA"
                     /locus_tag="J8I47_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAZ6321741.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            28222..28785
                     /gene="grpE"
                     /locus_tag="J8I47_000274"
     CDS             28222..28785
                     /gene="grpE"
                     /locus_tag="J8I47_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAZ6321742.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            28836..30665
                     /gene="dnaK"
                     /locus_tag="J8I47_000275"
     CDS             28836..30665
                     /gene="dnaK"
                     /locus_tag="J8I47_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAZ6321743.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            30816..31982
                     /gene="dnaJ"
                     /locus_tag="J8I47_000276"
     CDS             30816..31982
                     /gene="dnaJ"
                     /locus_tag="J8I47_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAZ6321744.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(32064..32729)
                     /locus_tag="J8I47_000277"
     CDS             complement(32064..32729)
                     /locus_tag="J8I47_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAZ6321745.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            32705..32878
                     /locus_tag="J8I47_000278"
     CDS             32705..32878
                     /locus_tag="J8I47_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ6321746.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(32923..33660)
                     /locus_tag="J8I47_000279"
     CDS             complement(32923..33660)
                     /locus_tag="J8I47_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321747.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(33662..35164)
                     /locus_tag="J8I47_000280"
     CDS             complement(33662..35164)
                     /locus_tag="J8I47_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ6321748.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            35423..37417
                     /gene="uvrB"
                     /locus_tag="J8I47_000281"
     CDS             35423..37417
                     /gene="uvrB"
                     /locus_tag="J8I47_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAZ6321749.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            37427..40243
                     /gene="uvrA"
                     /locus_tag="J8I47_000282"
     CDS             37427..40243
                     /gene="uvrA"
                     /locus_tag="J8I47_000282"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAZ6321750.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            40506..41771
                     /locus_tag="J8I47_000283"
     CDS             40506..41771
                     /locus_tag="J8I47_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAZ6321751.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            41775..42506
                     /locus_tag="J8I47_000284"
     CDS             41775..42506
                     /locus_tag="J8I47_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAZ6321752.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            42641..43525
                     /gene="rapZ"
                     /locus_tag="J8I47_000285"
     CDS             42641..43525
                     /gene="rapZ"
                     /locus_tag="J8I47_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAZ6321753.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            43522..44520
                     /locus_tag="J8I47_000286"
     CDS             43522..44520
                     /locus_tag="J8I47_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAZ6321754.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            44554..45489
                     /gene="whiA"
                     /locus_tag="J8I47_000287"
     CDS             44554..45489
                     /gene="whiA"
                     /locus_tag="J8I47_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAZ6321755.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            46032..46682
                     /locus_tag="J8I47_000288"
     CDS             46032..46682
                     /locus_tag="J8I47_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321756.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            46869..>47429
                     /locus_tag="J8I47_000289"
     CDS             46869..>47429
                     /locus_tag="J8I47_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAZ6321757.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        15919 a         8148 c        10182 g        13180 t
ORIGIN      
        1 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
       61 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
      121 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
      181 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg
      241 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
      301 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
      361 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
      421 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
      481 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
      541 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
      601 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
      661 actttgctgg acccatgaat aactttattt tggcaatcct gttattcatc attctggcat
      721 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
      781 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
      841 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag
      901 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
      961 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     1021 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     1081 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     1141 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     1201 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     1261 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     1321 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     1381 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     1441 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     1501 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     1561 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     1621 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     1681 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     1741 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     1801 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     1861 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     1921 cttcgcggaa gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt
     1981 ttggacgaga catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc
     2041 ttagaatttc gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa
     2101 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     2161 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     2221 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     2281 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     2341 gtccttgttt tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     2401 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     2461 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     2521 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     2581 gtgaatccag aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa
     2641 ggagatcctt caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga
     2701 catatcttca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     2761 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     2821 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg
     2881 ccatttgatt tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg
     2941 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     3001 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     3061 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     3121 gttgaagtac gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa
     3181 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     3241 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     3301 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     3361 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     3421 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     3481 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca
     3541 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     3601 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     3661 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     3721 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     3781 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     3841 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     3901 agcggaacga gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     3961 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     4021 atcgccagct cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga
     4081 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     4141 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     4201 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     4261 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     4321 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     4381 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     4441 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg
     4501 acaccgagct atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag
     4561 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     4621 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt
     4681 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     4741 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     4801 tcctttgtca cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc
     4861 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     4921 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     4981 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     5041 attcaaacgt ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca
     5101 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     5161 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     5221 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     5281 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     5341 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     5401 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     5461 agcaaaattc tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     5521 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     5581 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     5641 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     5701 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     5761 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     5821 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     5881 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     5941 tgagatcatt caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc
     6001 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     6061 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     6121 taatccgtta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     6181 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     6241 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     6301 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     6361 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     6421 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     6481 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     6541 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     6601 atatccagca gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat
     6661 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     6721 gcttcaagat ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat
     6781 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     6841 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac
     6901 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     6961 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     7021 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     7081 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     7141 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     7201 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     7261 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     7321 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     7381 aatgaaagaa atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac
     7441 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     7501 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     7561 agctgtccca agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg
     7621 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     7681 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     7741 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     7801 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     7861 ggaattacgc acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt
     7921 cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt
     7981 tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga
     8041 tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc
     8101 agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt
     8161 gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca
     8221 accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct
     8281 tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat
     8341 tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta
     8401 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
     8461 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
     8521 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
     8581 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
     8641 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
     8701 ggcggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
     8761 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
     8821 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
     8881 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
     8941 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
     9001 tattgcgccg cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg
     9061 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
     9121 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
     9181 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
     9241 ttgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
     9301 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
     9361 aaaacgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
     9421 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
     9481 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
     9541 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
     9601 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
     9661 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
     9721 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
     9781 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
     9841 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
     9901 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
     9961 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    10021 aagcgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    10081 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    10141 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    10201 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    10261 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    10321 tagcaatgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    10381 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    10441 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    10501 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    10561 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    10621 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    10681 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    10741 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    10801 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    10861 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    10921 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    10981 agaagaagtc gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca
    11041 cgtagaccat gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg
    11101 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    11161 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    11221 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    11281 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    11341 aattgataaa ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt
    11401 aattcctgaa gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca
    11461 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    11521 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    11581 tcctgtgtca accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt
    11641 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    11701 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    11761 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa
    11821 acgtgcatta ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga
    11881 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    11941 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    12001 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    12061 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    12121 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    12181 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    12241 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    12301 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    12361 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    12421 tgaatgtggt gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    12481 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    12541 gctaactacc gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    12601 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    12661 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    12721 aaaacagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    12781 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    12841 ggcaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca
    12901 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    12961 agttccatga ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    13021 aaaaattcga ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa
    13081 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
    13141 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
    13201 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
    13261 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
    13321 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
    13381 cgatccagcc ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat
    13441 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
    13501 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
    13561 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
    13621 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
    13681 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
    13741 ctttatctta aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa
    13801 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
    13861 cgagtcatca ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga
    13921 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
    13981 acggaagatg agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt
    14041 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
    14101 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
    14161 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
    14221 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
    14281 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
    14341 cttggctggg aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg
    14401 gctgaaaaaa tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg
    14461 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
    14521 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
    14581 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
    14641 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
    14701 agagaaatcg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
    14761 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
    14821 ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
    14881 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
    14941 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
    15001 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
    15061 gggttcttag aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat
    15121 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg
    15181 acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc
    15241 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    15301 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    15361 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    15421 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    15481 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    15541 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    15601 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    15661 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    15721 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    15781 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    15841 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    15901 gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata
    15961 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    16021 aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa
    16081 aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt
    16141 tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc
    16201 aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta
    16261 cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg
    16321 tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa
    16381 tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat
    16441 gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg
    16501 cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc
    16561 gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca
    16621 acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct
    16681 gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca
    16741 atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa
    16801 acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg
    16861 acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga
    16921 acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg
    16981 tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa
    17041 gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa
    17101 ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac
    17161 ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct
    17221 ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa
    17281 ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga
    17341 catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc
    17401 gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa
    17461 gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag
    17521 atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc
    17581 gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca
    17641 ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt
    17701 tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc
    17761 aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc
    17821 tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta
    17881 ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta
    17941 ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt
    18001 tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat
    18061 aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat
    18121 caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg
    18181 aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct
    18241 gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc
    18301 aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc
    18361 acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa
    18421 atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact
    18481 agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg
    18541 tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
    18601 ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
    18661 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
    18721 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
    18781 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
    18841 gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa
    18901 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat
    18961 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa
    19021 aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct
    19081 cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat
    19141 gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat
    19201 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
    19261 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
    19321 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
    19381 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
    19441 gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
    19501 atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct
    19561 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt
    19621 attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
    19681 atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa
    19741 tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt
    19801 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt
    19861 agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt
    19921 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
    19981 atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg
    20041 atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
    20101 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
    20161 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
    20221 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
    20281 ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
    20341 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga
    20401 aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt
    20461 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
    20521 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg
    20581 attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag
    20641 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
    20701 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
    20761 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
    20821 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
    20881 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
    20941 gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta
    21001 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
    21061 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactatgta
    21121 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
    21181 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
    21241 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
    21301 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
    21361 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
    21421 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
    21481 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
    21541 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
    21601 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
    21661 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
    21721 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
    21781 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
    21841 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
    21901 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
    21961 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
    22021 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
    22081 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
    22141 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
    22201 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
    22261 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
    22321 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
    22381 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
    22441 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
    22501 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
    22561 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
    22621 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
    22681 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
    22741 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
    22801 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
    22861 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
    22921 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
    22981 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
    23041 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
    23101 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
    23161 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
    23221 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
    23281 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
    23341 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
    23401 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
    23461 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
    23521 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
    23581 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
    23641 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
    23701 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
    23761 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
    23821 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
    23881 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
    23941 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
    24001 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
    24061 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
    24121 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
    24181 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
    24241 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
    24301 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
    24361 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
    24421 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
    24481 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
    24541 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
    24601 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
    24661 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
    24721 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
    24781 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
    24841 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
    24901 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
    24961 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
    25021 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
    25081 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
    25141 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
    25201 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
    25261 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
    25321 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
    25381 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
    25441 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
    25501 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
    25561 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
    25621 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
    25681 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
    25741 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
    25801 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
    25861 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
    25921 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
    25981 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
    26041 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
    26101 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
    26161 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
    26221 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
    26281 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
    26341 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
    26401 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
    26461 cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct
    26521 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
    26581 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
    26641 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
    26701 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
    26761 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
    26821 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
    26881 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
    26941 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
    27001 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
    27061 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
    27121 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
    27181 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
    27241 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
    27301 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
    27361 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
    27421 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
    27481 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
    27541 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
    27601 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
    27661 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
    27721 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
    27781 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
    27841 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
    27901 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
    27961 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
    28021 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
    28081 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
    28141 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
    28201 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
    28261 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
    28321 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
    28381 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
    28441 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
    28501 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
    28561 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
    28621 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
    28681 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
    28741 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
    28801 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
    28861 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
    28921 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
    28981 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
    29041 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
    29101 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
    29161 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
    29221 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
    29281 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
    29341 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
    29401 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
    29461 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
    29521 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
    29581 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
    29641 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
    29701 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
    29761 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
    29821 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
    29881 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
    29941 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
    30001 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
    30061 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
    30121 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
    30181 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
    30241 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
    30301 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
    30361 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
    30421 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
    30481 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
    30541 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
    30601 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
    30661 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
    30721 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
    30781 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
    30841 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
    30901 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
    30961 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
    31021 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    31081 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    31141 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
    31201 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    31261 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    31321 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    31381 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    31441 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    31501 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    31561 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    31621 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    31681 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    31741 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    31801 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    31861 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    31921 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    31981 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    32041 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    32101 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    32161 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    32221 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    32281 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    32341 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    32401 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    32461 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    32521 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    32581 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    32641 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    32701 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    32761 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    32821 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    32881 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    32941 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    33001 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    33061 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    33121 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    33181 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    33241 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    33301 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    33361 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    33421 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    33481 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    33541 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    33601 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    33661 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    33721 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    33781 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    33841 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    33901 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    33961 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    34021 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    34081 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    34141 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    34201 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    34261 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    34321 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    34381 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    34441 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    34501 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    34561 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    34621 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    34681 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    34741 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    34801 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    34861 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    34921 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    34981 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    35041 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    35101 acgcaaatgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    35161 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    35221 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    35281 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    35341 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    35401 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    35461 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    35521 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    35581 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    35641 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    35701 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    35761 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    35821 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    35881 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    35941 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    36001 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    36061 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    36121 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    36181 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    36241 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    36301 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    36361 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    36421 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    36481 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    36541 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    36601 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    36661 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    36721 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    36781 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    36841 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    36901 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    36961 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    37021 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    37081 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    37141 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    37201 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    37261 agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa
    37321 attagaaaaa gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt
    37381 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    37441 aattacgatt catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    37501 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    37561 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    37621 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    37681 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    37741 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    37801 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    37861 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    37921 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    37981 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    38041 catcgaccgg atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc
    38101 ggccctgcga ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    38161 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    38221 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    38281 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    38341 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    38401 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    38461 gatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    38521 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    38581 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    38641 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    38701 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    38761 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    38821 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    38881 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    38941 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    39001 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    39061 tgatgaagat acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga
    39121 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    39181 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    39241 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    39301 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    39361 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    39421 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    39481 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    39541 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    39601 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    39661 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    39721 taaacgatat aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    39781 tttggaaatg acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg
    39841 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    39901 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    39961 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    40021 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    40081 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    40141 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    40201 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    40261 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    40321 gaatcattac atggtagttc tttctacact gattggtata gataaaaact tcctataagt
    40381 tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt
    40441 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    40501 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    40561 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    40621 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    40681 caggatttga taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat
    40741 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    40801 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    40861 tatttgaaag gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    40921 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    40981 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    41041 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    41101 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    41161 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    41221 ggatgatgtg ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt
    41281 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    41341 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    41401 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    41461 acggcttgaa tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg
    41521 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    41581 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    41641 aagaaaatcg ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt
    41701 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    41761 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    41821 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    41881 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    41941 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    42001 gaccaaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag
    42061 cggcaacgga tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc
    42121 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    42181 atgaacgcga agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag
    42241 aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    42301 tctattacga aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag
    42361 gttgcacaga attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg
    42421 atgctcaatc tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg
    42481 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat
    42541 atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg cccgggatga
    42601 tataatttta ttatgcacaa tgtgagaggg gttcgtaaat atgacagata atttgcaatt
    42661 agtcatcata acaggtatga gtggtgcagg gaaaacagta gctatccaaa gttttgaaga
    42721 tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt
    42781 gatcaaagaa tcaggtaaag tcacaaaaat ggctttagga gtggatttgc gttctcgttc
    42841 cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac
    42901 ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag
    42961 acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc
    43021 gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacaa cattgacacc
    43081 acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg
    43141 gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat
    43201 ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa
    43261 agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa
    43321 atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt
    43381 gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt
    43441 ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag
    43501 gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc
    43561 gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttacg aaatcaagga
    43621 gcagatatca cagcagtagt gactgttgca gatgatggtg gaagcagcgg tgcaatcaga
    43681 gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat
    43741 atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta
    43801 gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact
    43861 tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct
    43921 tcagaaactc ctttgacctt gcatgctgtt tttaaagacg gaacgactgc agtaggagaa
    43981 tctaaaatcg cgatcaaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat
    44041 aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc
    44101 gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc
    44161 ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa
    44221 aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg
    44281 aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg
    44341 gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga
    44401 aatgaaggat gtcgagtgat ttccaccgac tttttagaat tgaaagatgg tggtgtcttc
    44461 cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa
    44521 gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa
    44581 gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg
    44641 cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa
    44701 aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt
    44761 gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg
    44821 aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat
    44881 ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt
    44941 gcttttatgg cctcaggttc agtcaataat ccggaaacga gtcgttatca tttggaaata
    45001 ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta
    45061 catgctcgaa cgcttgaacg gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa
    45121 attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt
    45181 cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat
    45241 ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca
    45301 aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa
    45361 catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc aatatcaaaa
    45421 tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa
    45481 gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt
    45541 ttttaaaagc tatacttgct tttcttccgt caaactataa ttccaacttt actggagtaa
    45601 cataatgttg aaaggcttct attgtttttt aatacaatcg tataaaaagc ggggagacaa
    45661 ctgaatgaag attttagttt tatacataaa cttttgctta atatagaagt cctgatatag
    45721 ataggatcaa gtatttatct ataactggag attaattaaa aactggaagg aactgctcga
    45781 tcaaccttga gaactgtttt tcttacttct ggtcaagatt ctcgtggttg gtggataagg
    45841 atggttgata agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa
    45901 gttttaacaa ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat
    45961 tgggatgtat ggttggtata cgttatacct gaacttgaac tatcaagatt tggtttagaa
    46021 tttgagaatc gatgtttata aagggagggc aaaaaatgtg cttttttaca acttttacta
    46081 tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt
    46141 tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt
    46201 ttctattcac agttattgta tttatttgga gaactacagc accaccattg ccagagatta
    46261 catatggaga atttccgttt agtcttgaat atgaaataga tgaggaaaga attttgattg
    46321 aagatacggt gattgcagaa tttataggaa gttttagagg gaatgtgaca actcaaccta
    46381 gacgaagttg gagtataaaa cttttaaata atagtggaga gttgaaagca tttcattatg
    46441 aattcttaat aatagaaagt gaagatgtaa caatcacttt aagtagtggc tctgctggat
    46501 attttatgaa tgatggtgga agcaagccac agctcacagt ccgtgatcgt attgcaggtt
    46561 ttatgcattt ttctcactat gacttagaag aagctcgtaa aatacttgca gaatatggga
    46621 tcacattaat caattgggaa tatgcgccac cgataagaaa ttttttcaat gattattatt
    46681 agtcttataa aaaacagaat gtaaaattta tgtgcgcgag tatttagttt tccatttttt
    46741 gtttgataga tatatagcat tgttgaagca tgggatagtg gtgccaattt tatcaatgag
    46801 gttggtcagg cagttaacat aattggtgat gctataaatc catttaattg gaattggagg
    46861 aattaggtgt ggaaacagtg agattatgga tattcatgaa aaattttcgt tcggctttga
    46921 gatatgttat tctggaatca aaatcaggtt attatctgat tgacacagat cactatactt
    46981 tttggggagg ctaccttttt ccgattttta attggataat cccccataag gttatgaaaa
    47041 ttaacttgcc tgcagaagaa ataaatagat taatactcaa caaagattta caaaggaaaa
    47101 gagagaaaga acattctaat gttgcttgga taggtccagc atttgcagta gtgacttctt
    47161 caattgtcgg tcctatgcta gagagtattt atagtgattt ttctattgga atacgttatt
    47221 tatttgttac tttaagtata gctgttcttt tgtgcataaa gggttatgtt agcaagaaga
    47281 ataaagatat tttaacacta atcggtgtaa acaatttatc tagtactaaa attatactgt
    47341 ttcctgaatc tataaaagaa ggatcaaggt tgttgataat ttttataatg cttatactgt
    47401 tttctgtttt atttggggtg atgtttata
//
LOCUS       DADOZM010000006        43805 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.006, whole genome shotgun sequence.
ACCESSION   DADOZM010000006 DADOZM010000000
VERSION     DADOZM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.006"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(16..360)
                     /locus_tag="J8I47_000290"
     CDS             complement(16..360)
                     /locus_tag="J8I47_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAZ6321758.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(353..787)
                     /locus_tag="J8I47_000291"
     CDS             complement(353..787)
                     /locus_tag="J8I47_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAZ6321759.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            925..1662
                     /locus_tag="J8I47_000292"
     CDS             925..1662
                     /locus_tag="J8I47_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321760.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1655..2866
                     /locus_tag="J8I47_000293"
     CDS             1655..2866
                     /locus_tag="J8I47_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321761.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3073..3852
                     /locus_tag="J8I47_000294"
     CDS             3073..3852
                     /locus_tag="J8I47_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAZ6321762.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            3922..4584
                     /gene="trmB"
                     /locus_tag="J8I47_000295"
     CDS             3922..4584
                     /gene="trmB"
                     /locus_tag="J8I47_000295"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAZ6321763.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4682..6436)
                     /gene="ade"
                     /locus_tag="J8I47_000296"
     CDS             complement(4682..6436)
                     /gene="ade"
                     /locus_tag="J8I47_000296"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAZ6321764.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6568..7497
                     /gene="cpaF"
                     /locus_tag="J8I47_000297"
     CDS             6568..7497
                     /gene="cpaF"
                     /locus_tag="J8I47_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAZ6321765.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7454..8503
                     /locus_tag="J8I47_000298"
     CDS             7454..8503
                     /locus_tag="J8I47_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAZ6321766.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8500..8778
                     /locus_tag="J8I47_000299"
     CDS             8500..8778
                     /locus_tag="J8I47_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAZ6321767.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8753..9223
                     /locus_tag="J8I47_000300"
     CDS             8753..9223
                     /locus_tag="J8I47_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAZ6321768.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9240..9542
                     /locus_tag="J8I47_000301"
     CDS             9240..9542
                     /locus_tag="J8I47_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321769.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9559..9930
                     /locus_tag="J8I47_000302"
     CDS             9559..9930
                     /locus_tag="J8I47_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAZ6321770.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9902..10261
                     /locus_tag="J8I47_000303"
     CDS             9902..10261
                     /locus_tag="J8I47_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321771.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10433..11440
                     /locus_tag="J8I47_000304"
     CDS             10433..11440
                     /locus_tag="J8I47_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6321772.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11465..12649
                     /locus_tag="J8I47_000305"
     CDS             11465..12649
                     /locus_tag="J8I47_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAZ6321773.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13086..14669)
                     /locus_tag="J8I47_000306"
     CDS             complement(13086..14669)
                     /locus_tag="J8I47_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ6321774.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15247..15390)
                     /locus_tag="J8I47_000307"
     CDS             complement(15247..15390)
                     /locus_tag="J8I47_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAZ6321775.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15612..16091)
                     /locus_tag="J8I47_000308"
     CDS             complement(15612..16091)
                     /locus_tag="J8I47_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ6321776.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16164..16448)
                     /locus_tag="J8I47_000309"
     CDS             complement(16164..16448)
                     /locus_tag="J8I47_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321777.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16526..16720)
                     /gene="ssrS"
                     /locus_tag="J8I47_000310"
     ncRNA           complement(16526..16720)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J8I47_000310"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16792..18078)
                     /locus_tag="J8I47_000311"
     CDS             complement(16792..18078)
                     /locus_tag="J8I47_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAZ6321778.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18216..18887
                     /locus_tag="J8I47_000312"
     CDS             18216..18887
                     /locus_tag="J8I47_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAZ6321779.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18853..19341
                     /locus_tag="J8I47_000313"
     CDS             18853..19341
                     /locus_tag="J8I47_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAZ6321780.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19354..20301
                     /gene="prmA"
                     /locus_tag="J8I47_000314"
     CDS             19354..20301
                     /gene="prmA"
                     /locus_tag="J8I47_000314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAZ6321781.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20302..21066
                     /locus_tag="J8I47_000315"
     CDS             20302..21066
                     /locus_tag="J8I47_000315"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAZ6321782.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21299..23512
                     /locus_tag="J8I47_000316"
     CDS             21299..23512
                     /locus_tag="J8I47_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAZ6321783.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23523..23969
                     /locus_tag="J8I47_000317"
     CDS             23523..23969
                     /locus_tag="J8I47_000317"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAZ6321784.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24140..25399
                     /locus_tag="J8I47_000318"
     CDS             24140..25399
                     /locus_tag="J8I47_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAZ6321785.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25488..26435)
                     /locus_tag="J8I47_000319"
     CDS             complement(25488..26435)
                     /locus_tag="J8I47_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAZ6321786.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26829..28121
                     /locus_tag="J8I47_000320"
     CDS             26829..28121
                     /locus_tag="J8I47_000320"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAZ6321787.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28136..29905
                     /gene="aspS"
                     /locus_tag="J8I47_000321"
     CDS             28136..29905
                     /gene="aspS"
                     /locus_tag="J8I47_000321"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAZ6321788.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30014..30889
                     /locus_tag="J8I47_000322"
     CDS             30014..30889
                     /locus_tag="J8I47_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6321789.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31155..33038
                     /locus_tag="J8I47_000323"
     CDS             31155..33038
                     /locus_tag="J8I47_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAZ6321790.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33113..33397)
                     /locus_tag="J8I47_000324"
     CDS             complement(33113..33397)
                     /locus_tag="J8I47_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAZ6321791.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33387..34121)
                     /locus_tag="J8I47_000325"
     CDS             complement(33387..34121)
                     /locus_tag="J8I47_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAZ6321792.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34250..34873
                     /locus_tag="J8I47_000326"
     CDS             34250..34873
                     /locus_tag="J8I47_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAZ6321793.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35065..36186
                     /locus_tag="J8I47_000327"
     CDS             35065..36186
                     /locus_tag="J8I47_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAZ6321794.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36193..39321
                     /locus_tag="J8I47_000328"
     CDS             36193..39321
                     /locus_tag="J8I47_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAZ6321795.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39340..42534
                     /locus_tag="J8I47_000329"
     CDS             39340..42534
                     /locus_tag="J8I47_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ6321796.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42680..43387)
                     /locus_tag="J8I47_000330"
     CDS             complement(42680..43387)
                     /locus_tag="J8I47_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ6321797.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14144 a         7763 c         9238 g        12660 t
ORIGIN      
        1 agaaaataat cattattata tttgttcagg atactctggc agatcagctt ttagtttttc
       61 tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc
      121 ttttttgatt gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat
      181 caatgcgctt ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc
      241 ttccaattct tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag
      301 acctccgaca gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat
      361 cgcaggacac ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac
      421 tatcttgatg attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta
      481 aatggatatg tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat
      541 taagaccttt catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg
      601 aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag
      661 gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt
      721 cgtcttcata tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt
      781 tttccatata aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag
      841 ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata
      901 aaaataaatg gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac
      961 ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg
     1021 ttgattggac tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg
     1081 gaagcttcaa aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa
     1141 tatcgcaaac aaattgggta tattccagag actccttctt tatatgaaga attgacattg
     1201 agagaacata tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa
     1261 agagcagaaa agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct
     1321 aatttttcaa agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct
     1381 tctttatata tcatcgacga accattttta ggcttggatc cactagctat caatgcattg
     1441 cttgaattga tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt
     1501 ttagctactg cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga
     1561 gcagacggtt ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac
     1621 gaaatctata ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt
     1681 agcaagacat caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt
     1741 tttagtttgt cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac
     1801 cctgcagcca ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt
     1861 attcgtgggt aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa
     1921 agaaaaagaa atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt
     1981 gatctttttg atattggcat gcggattggc catgccatta gtggtggtta gtacggagca
     2041 atccttttcg atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct
     2101 gcaaatcaag agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt
     2161 aggctggttt ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc
     2221 aggaatagca ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca
     2281 agaaaaagga ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat
     2341 ctatcaattt atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag
     2401 acgctatttg gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta
     2461 cttgtttgca agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt
     2521 aattataggt gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg
     2581 tttcttattt ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta
     2641 tatggttctc gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta
     2701 tctactcaca tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt
     2761 attagatggg cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt
     2821 tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt
     2881 agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt
     2941 tttttgagaa aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt
     3001 gacgtgtagt gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg
     3061 ggagaatgca tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc
     3121 gacacaggaa aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac
     3181 acgactccga tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca
     3241 aaacgaacgg ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg
     3301 actcctaatg agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat
     3361 tcaaattctc ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag
     3421 atgctgcaaa aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa
     3481 attgtccatc gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta
     3541 gtacacggag atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc
     3601 gattgggatt caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat
     3661 tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac
     3721 gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga
     3781 tattgcaaac gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt
     3841 ttcagtggct gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga
     3901 ggaaaggaat ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta
     3961 gcggctcacc cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa
     4021 ttgtttgaaa acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc
     4081 actggaatgg caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt
     4141 gttgtttcga ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta
     4201 catgtggatg gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat
     4261 ctgaattttt ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag
     4321 acttttcttg ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca
     4381 gataatcaag gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc
     4441 aaacaggtat ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat
     4501 gaagaaaaat tttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat
     4561 aagaccaaaa agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc
     4621 ataaataaat ctcagaaaat gaacattaca gatgtaatgt attttctggg atttatttga
     4681 tttaaaatgt atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa
     4741 atgaaaataa tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag
     4801 aaagtgtcaa aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta
     4861 attcattcaa ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag
     4921 gaagagaaac acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt
     4981 gaatcaaaag attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat
     5041 tatgcgagtc atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca
     5101 ctatgcctaa tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt
     5161 ggtctttttc gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat
     5221 ctttagtcac tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa
     5281 gcagctctaa cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat
     5341 atgaatttga atgatcagca aataaatggt cttggagttg cccagattga acaactaatg
     5401 ttcccttata gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat
     5461 cagcttgata cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat
     5521 tcaatgttgc catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga
     5581 cgatatgatc gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa
     5641 agaagcatct tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg
     5701 caacggtccc ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt
     5761 ttgcagttgt agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt
     5821 ccatgccgat accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga
     5881 tcttttctag gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta
     5941 agcctaaaac ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac
     6001 cattggtctc aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga
     6061 gtgtttgtct tgcatcttct atcataaact caagacctgt actcccagca atgtttccta
     6121 tttcatgggg atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt
     6181 cgcttggtga taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta
     6241 gatattgccc attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca
     6301 ttccttcgca gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct
     6361 tagcatgctt gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc
     6421 ttaatgttgt ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt
     6481 cggcttttgc caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga
     6541 cgtatgtttt ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata
     6601 agtgaaagtt ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt
     6661 tacatctatt ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat
     6721 ggacagcaac tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga
     6781 aaagctcagt taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg
     6841 tcttctgtag gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact
     6901 tttgaaacac cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc
     6961 gtcagacgtg gattatacct tttcagcggg acagtcggat caggcaagac ttctctaatg
     7021 tatcggttgg ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata
     7081 gaagaaccgt tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca
     7141 cttttgaaac tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat
     7201 gaatcaactg cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc
     7261 gtacatgcac gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa
     7321 gaagagctat ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat
     7381 caccatcctt ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca
     7441 tgggaggaaa gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg
     7501 attaacaaga agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg
     7561 ttttagtctg caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat
     7621 tttggcaaat atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca
     7681 aataggatat ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct
     7741 gccgacaact ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga
     7801 tttgaaaaag ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt
     7861 tagtatgcgg ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca
     7921 ttggggtatt cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt
     7981 tactctattg gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag
     8041 tgaatggata agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta
     8101 tttgtcacta gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt
     8161 ctgcttaaag gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca
     8221 aggattggag gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga
     8281 agaattcagt aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat
     8341 tttttacagc gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt
     8401 tattcagcca atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt
     8461 tttattacct gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct
     8521 tattatagcg gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta
     8581 gtgttgttgt tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt
     8641 gaagcgatca tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga
     8701 agagttccta ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa
     8761 atctatcagc ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact
     8821 cttactgatt ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg
     8881 gaagaatgaa ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac
     8941 gcaaaaaatc gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa
     9001 tcaaatcatt tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc
     9061 tgatgagatt accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga
     9121 aagttcacta aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca
     9181 attccagata gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt
     9241 tggagtccct tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc
     9301 agctgttttt gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga
     9361 tttatgaaga tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa
     9421 tgatcccata cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca
     9481 atatggagaa agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt
     9541 gaatgtctaa ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt
     9601 cagcaaacca tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata
     9661 tttttagcac aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag
     9721 caactgcact acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata
     9781 cgtaaaagaa gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa
     9841 ttattggaaa aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga
     9901 tatgccatat ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc
     9961 tgtcttgctt ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg
    10021 tttgtcaatg gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca
    10081 gatggcttta tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac
    10141 aactggatct gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc
    10201 accgtttatc tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg
    10261 atggaatgtt cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg
    10321 tttgccgttt cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata
    10381 gaattaaaca ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc
    10441 agaaaaaatg gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc
    10501 attgggaact tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta
    10561 tcaagtacgt gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct
    10621 ttatgaagag ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt
    10681 acttttattg aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga
    10741 tagtatcggt tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc
    10801 ggttaagatt ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa
    10861 cctatcgaat gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt
    10921 ggctgtcgca gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga
    10981 cgggttgcag gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg
    11041 ttattatccg aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag
    11101 ttatgcacat catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg
    11161 tttgtttttg atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg
    11221 gtttaaagaa gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa
    11281 taaacaatcg caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt
    11341 aaaagaagtc ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt
    11401 tttcaacgag tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag
    11461 tcatatgtct aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata
    11521 tcaaatgcca gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga
    11581 ttctattttt accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga
    11641 aaaccacgaa gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag
    11701 ctcttatgat gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg
    11761 ggattcagta gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc
    11821 tcctttgcat aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga
    11881 cattttgagt gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta
    11941 tttatatagc ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca
    12001 tggcacaagt catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga
    12061 agaactaaaa atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg
    12121 aggtaaatcc atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac
    12181 acgttcaggt gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga
    12241 tattgatgaa atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt
    12301 gtctagtgat atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta
    12361 tgacattttc gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg
    12421 tgttgatgca attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga
    12481 aatcatcaaa ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg
    12541 tggtgaagaa gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac
    12601 agatgaggaa ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt
    12661 cgttaggata aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg
    12721 agaagaccgc aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta
    12781 gggaattttg cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt
    12841 tcaggaaaat cccaatattt tattttcatt catctttata agggacatta catatgtaaa
    12901 aagaaaaaac atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt
    12961 tagctagaaa accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc
    13021 tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa
    13081 acgtcctagt caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg
    13141 agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt
    13201 ccttgatagt cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg
    13261 gttggttcat ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa
    13321 cggatttttt caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag
    13381 ccacttttgg ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa
    13441 gccagcaaac ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc
    13501 aagctactac ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg
    13561 agtaaggttg attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag
    13621 actgcaaaac tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga
    13681 atgatgaaaa cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc
    13741 gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct
    13801 tttttgaaga aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg
    13861 actttcaacc gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag
    13921 cgagcttctt tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct
    13981 tcttccagtt cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg
    14041 accagaaccg atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga
    14101 tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg
    14161 gctaaagcga ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacag
    14221 tgtggaatcc ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct
    14281 agttcttcaa agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta
    14341 tctggttctg tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca
    14401 gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg
    14461 accgtcccga tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg
    14521 ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt tgatatcgac taaggcaatt
    14581 ttttcaccag cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt
    14641 tttttaatag aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc
    14701 catggttatc cctggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag
    14761 atggccattg attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa
    14821 ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg
    14881 ttattttagt catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg
    14941 tacctgaaag tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg
    15001 taattctgtc agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat
    15061 tgtattctga gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg
    15121 tctacatgac ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt
    15181 ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc
    15241 ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa
    15301 cccgaaataa aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt
    15361 tttcgtatca gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt
    15421 aggtcgtttt aaaccacacc agggcgacta atcgccctgg tgttcacttt ttttatttta
    15481 cttgaactca ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg
    15541 ccgcgtcttt tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg
    15601 tcgtctacgt gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt
    15661 cggacaacaa gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata
    15721 aataaacgtt ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta
    15781 tcttctggga tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt
    15841 acattattca agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc
    15901 atttcagtag cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt
    15961 gtgtcaatga catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc
    16021 tttttaaaag ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat
    16081 tgttgtaaca taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac
    16141 actttaggga aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg
    16201 atactgaagg tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt
    16261 tgattgaaaa aagctgttaa aatacccatt acgcctaaaa cagcatatgc agcaccgtaa
    16321 aattgtagga atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt
    16381 ttattaaata aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc
    16441 ctaatcatga acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc
    16501 aaattgcttt attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt
    16561 attgatcccg cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga
    16621 taaggcatgt taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg
    16681 tcaacacaca aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg
    16741 aatttaaaaa gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt
    16801 tggaggatgc tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata
    16861 tttccctgta tcttttggct gataatactg agcattcttc agtttatctg gtaaatattg
    16921 ctgattgacc caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag
    16981 tgatttcgca cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc
    17041 ctctcgaata tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga
    17101 caagcaaaga tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt
    17161 ttcagcagct aatacagcgt tcactgtacg agccgcagca gcaggattac ctaaacctat
    17221 atcttcataa ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac
    17281 taaacgggcg agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa
    17341 agccgaaata acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg
    17401 gacacattct tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt
    17461 cgatagtgta gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg
    17521 cgaaaggtga agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc
    17581 tttatccctt aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac
    17641 ttcaaagatc tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt
    17701 agtcgctccg atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt
    17761 tgttttatct aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc
    17821 ttcttccgct acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa
    17881 tgcataatta gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg
    17941 tccatataga atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc
    18001 ttccccgact agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata
    18061 agccaatggc tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc
    18121 atatttagca agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc
    18181 aactaatatt gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg
    18241 aatttttttg gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg
    18301 agcatgacac accagaagga agattagctg gatatattgt agacgcggaa gcatacctag
    18361 gtccagaaga cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa
    18421 tgtatgaaaa accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca
    18481 tcattactca gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg
    18541 caatgattga tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg
    18601 gtccgggaaa attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag
    18661 cagatagttc gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt
    18721 tgcctagaat cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg
    18781 tgtcgggaaa tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt
    18841 ggaggaaaga aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa
    18901 caagtgacag actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc
    18961 gttgttcgct tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc
    19021 gtttcgcaag aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa
    19081 tctgtttttg atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg
    19141 gcaaaatcag tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa
    19201 agcgatttgc ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg
    19261 gataatcaac aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat
    19321 ttaccgtacc caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa
    19381 actgaaagtg aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt
    19441 gtcgcgattg aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt
    19501 ttagataaaa atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg
    19561 gaaacgactt tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct
    19621 gagtttggtc tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac
    19681 tgggcaacag cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt
    19741 gcgccaagct gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat
    19801 cctggtatgg catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta
    19861 gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta
    19921 agtattgcag caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta
    19981 gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta
    20041 tcagctaacg atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt
    20101 ttagcagata tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc
    20161 acgctgatcg tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta
    20221 gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg
    20281 aaaaaaccag aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa
    20341 gactattttg ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa
    20401 cccgaggatg aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg
    20461 gagattactg aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa
    20521 ctgccaagtt cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc
    20581 gttcagaaat ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct
    20641 gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct
    20701 caagaggcag cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg
    20761 aaacaagatt tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa
    20821 tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat
    20881 agtcgaatct tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat
    20941 tttgtagaac acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca
    21001 gcacctcttt atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc
    21061 agctaacgtt cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca
    21121 atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt
    21181 ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc
    21241 aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt
    21301 gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg
    21361 tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg
    21421 acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt
    21481 agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga
    21541 agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt
    21601 agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc
    21661 agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa
    21721 attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag
    21781 aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag
    21841 ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta
    21901 ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac
    21961 tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg
    22021 gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga
    22081 aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt
    22141 attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc
    22201 catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa
    22261 tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc
    22321 tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa
    22381 acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga
    22441 atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa
    22501 aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca
    22561 tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga
    22621 ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc
    22681 aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt
    22741 cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg
    22801 tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc
    22861 actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga
    22921 agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat
    22981 cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa
    23041 aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg
    23101 tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg
    23161 cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt
    23221 agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga
    23281 tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac
    23341 agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat
    23401 ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga
    23461 taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag
    23521 atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa
    23581 ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa
    23641 aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag
    23701 gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca
    23761 cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag
    23821 aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag
    23881 tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa
    23941 cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg
    24001 aaacacgtcg cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa
    24061 aagcgcttct tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt
    24121 ttataggagg ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt
    24181 aaaaaaattg tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc
    24241 tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc
    24301 agcaaaagta ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt
    24361 attgaagaca ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca
    24421 tcggaaaaat atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat
    24481 cttagataag aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga
    24541 tttaagtaag atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc
    24601 gatttctaaa gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac
    24661 gtttacttct ccgattgatc acataaaagg tgtcgagaat cgcaaaaaat cagaaaaatg
    24721 ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc
    24781 attatatgct tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc
    24841 agctgttgaa ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt
    24901 gatgggacat gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc
    24961 gacgttacaa gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct
    25021 acctgcgatt tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg
    25081 ggttatttta gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc
    25141 ctttatgatc aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga
    25201 gatgattgca caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat
    25261 cactcttcat agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt
    25321 acctgaacaa gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc
    25381 gctactttac aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg
    25441 ctgatcttcc ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga
    25501 aatgcttcat caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca
    25561 ttgtcagata cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc
    25621 ttcatgtacc aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg
    25681 cgaagtacat cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt
    25741 ctttttcgag gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc
    25801 agcaaaccgg aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa
    25861 gggaataatt ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg
    25921 acatatccat ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact
    25981 gctcggattt ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt
    26041 gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt
    26101 agtgcatctt ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta
    26161 tttggtacat gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc
    26221 ttcagtacct cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg
    26281 atcaaattga tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc
    26341 aaaccgtcaa aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg
    26401 tagacttgtg aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt
    26461 ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata
    26521 tctcttgact ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag
    26581 agcgagtaag tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag
    26641 tttcattagc ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc
    26701 gttattcgta agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca
    26761 ttcacccttg ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag
    26821 agaaaactat gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag
    26881 aaaaatggca gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg
    26941 aaatgcgtac accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa
    27001 cggatatcgt ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc
    27061 ttcgaccaga aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc
    27121 cagagtatgc gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc
    27181 ctcaaaaagg acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa
    27241 accctgcaac tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga
    27301 tcacgcaaat ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc
    27361 gtcaagcatt gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac
    27421 gccgccttca tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat
    27481 ttgtggcaga agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg
    27541 ataccgttgt ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg
    27601 ttcgcggact agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa
    27661 tgggggcaca agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag
    27721 gtggtccttc aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta
    27781 tggaggcaga ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac
    27841 tcggagacga aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt
    27901 tttcagctga ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata
    27961 aagcgaatgc caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta
    28021 atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac
    28081 attttgatga agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc
    28141 acaaagaacg atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt
    28201 aaaaggatgg gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga
    28261 tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat
    28321 cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg
    28381 agaaaatgca gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat
    28441 cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag
    28501 tgacgaaata cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa
    28561 catcaaatta agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt
    28621 tctcgatatc gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct
    28681 agttccttca cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt
    28741 taaacaattg ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg
    28801 cgatgaagat ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac
    28861 gttcttaaca gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa
    28921 agaagttcgc ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat
    28981 ggctcgctac ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag
    29041 cgatacagtc aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt
    29101 cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca
    29161 gttaggtcaa tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga
    29221 agacggattg aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa
    29281 agcaacagat gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt
    29341 cgctgctgca ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga
    29401 atcgaaattc aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga
    29461 aggtcgatat gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt
    29521 attggctgaa gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta
    29581 tgaattaggc ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga
    29641 aactctaggc tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt
    29701 agattacgga ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt
    29761 attagccgga gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc
    29821 agatgtgatg agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat
    29881 tgatgtaacg aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc
    29941 ttcttttgac aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa
    30001 gcggggcttt tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa
    30061 gctttcggta tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca
    30121 gccaggacat atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc
    30181 aaaatcactg ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc
    30241 tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac
    30301 agtcactttg acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc
    30361 aatcatctgt gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag
    30421 aaacggccta tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg
    30481 aaagtcgatt ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat
    30541 tctatttggc tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt
    30601 aactgatgtc gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc
    30661 agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga
    30721 agcagaaggt gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata
    30781 cgaactgcct ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat
    30841 tgcagaaaac gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga
    30901 caaaaattca tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc
    30961 atagtagaat cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa
    31021 aagcatgttt atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac
    31081 gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa
    31141 agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt
    31201 ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta
    31261 gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca
    31321 cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg
    31381 tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt
    31441 atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct
    31501 tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg
    31561 ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg
    31621 tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt
    31681 atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag
    31741 gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg
    31801 gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag
    31861 cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct
    31921 tctttttaga tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt
    31981 tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga
    32041 caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc
    32101 cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata
    32161 ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc
    32221 gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca
    32281 aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg
    32341 cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc
    32401 aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca
    32461 ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga
    32521 ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa
    32581 cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat
    32641 atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc
    32701 tgattggact aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg
    32761 caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa
    32821 atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg
    32881 attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa
    32941 atcctgaagg gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt
    33001 caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca
    33061 aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag
    33121 attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga
    33181 gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat
    33241 ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt
    33301 tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt
    33361 tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca
    33421 gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat
    33481 ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa
    33541 acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag
    33601 aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa
    33661 atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta
    33721 ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag
    33781 tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc
    33841 atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt
    33901 tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg
    33961 ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca
    34021 agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct
    34081 tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa
    34141 aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac
    34201 ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg
    34261 atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga
    34321 gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg
    34381 gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa
    34441 agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc
    34501 agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc
    34561 gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg
    34621 cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg
    34681 tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc
    34741 aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg
    34801 aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt
    34861 caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt
    34921 cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt
    34981 tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag
    35041 aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga
    35101 aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta
    35161 gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg
    35221 agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta
    35281 aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca
    35341 gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca
    35401 tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt
    35461 gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag
    35521 gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat
    35581 ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg
    35641 gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat
    35701 aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct
    35761 ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag
    35821 ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa
    35881 gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat
    35941 ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt
    36001 attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta
    36061 ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag
    36121 cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg
    36181 gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat
    36241 gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca
    36301 ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc
    36361 ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca
    36421 agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa
    36481 caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg
    36541 accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat
    36601 cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta
    36661 ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta
    36721 cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca
    36781 aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg
    36841 tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg
    36901 acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa
    36961 cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa
    37021 agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac
    37081 gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa
    37141 ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt
    37201 gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa
    37261 gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa
    37321 cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta
    37381 acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc
    37441 tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa
    37501 gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa
    37561 caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact
    37621 gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg
    37681 aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag
    37741 ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca
    37801 aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa
    37861 agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta
    37921 tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac
    37981 ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact
    38041 cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa tgaaagaata
    38101 gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa
    38161 cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta
    38221 gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa
    38281 aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg
    38341 ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa
    38401 atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg
    38461 tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa
    38521 aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt
    38581 caattagaaa atatccagct aactgagaca ttcccagata cagaagagat cagccaacag
    38641 ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa
    38701 caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag
    38761 acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat
    38821 cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa
    38881 gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat
    38941 accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca
    39001 ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct
    39061 ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca
    39121 ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa
    39181 gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa
    39241 ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa
    39301 atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa
    39361 aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct
    39421 gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat
    39481 ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa
    39541 tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc
    39601 atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt
    39661 gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt
    39721 attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg
    39781 gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac
    39841 atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac
    39901 cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt
    39961 gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa
    40021 ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg
    40081 aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt
    40141 tctgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg
    40201 caagcattga atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg
    40261 acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt
    40321 cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa
    40381 gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag
    40441 gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc
    40501 caagctcgag tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag
    40561 gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt
    40621 gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca
    40681 agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt
    40741 gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg
    40801 aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata
    40861 gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca
    40921 ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg
    40981 ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat
    41041 caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc
    41101 caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga
    41161 ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt
    41221 cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca
    41281 attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg
    41341 cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt
    41401 atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat
    41461 atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa
    41521 attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca
    41581 caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag
    41641 aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact
    41701 aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt
    41761 ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta
    41821 tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt
    41881 gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct
    41941 ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa
    42001 ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa
    42061 cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta
    42121 gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta
    42181 agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct
    42241 gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg
    42301 aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg
    42361 gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag
    42421 caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa
    42481 ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact
    42541 cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat
    42601 ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt
    42661 ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa
    42721 aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga
    42781 accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc
    42841 caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct
    42901 ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc
    42961 aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga
    43021 taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat
    43081 gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt
    43141 ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat
    43201 attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga
    43261 accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg
    43321 cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt
    43381 taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt
    43441 gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta
    43501 tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac
    43561 gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat
    43621 gcaatctctg tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt
    43681 caaacataaa ataacgttat ttgatttatt tgtttatagt aaattttatt tagtaatcat
    43741 tgctttaaaa tactacgctt atataacaga gaaaataagc gttataataa agaacagtat
    43801 tattt
//
LOCUS       DADOZM010000007        43529 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.007, whole genome shotgun sequence.
ACCESSION   DADOZM010000007 DADOZM010000000
VERSION     DADOZM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.007"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(168..2549)
                     /locus_tag="J8I47_000331"
     CDS             complement(168..2549)
                     /locus_tag="J8I47_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAZ6321798.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2539..3186)
                     /gene="recU"
                     /locus_tag="J8I47_000332"
     CDS             complement(2539..3186)
                     /gene="recU"
                     /locus_tag="J8I47_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAZ6321799.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3250..3789
                     /locus_tag="J8I47_000333"
     CDS             3250..3789
                     /locus_tag="J8I47_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAZ6321800.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3879..4298
                     /gene="gpsB"
                     /locus_tag="J8I47_000334"
     CDS             3879..4298
                     /gene="gpsB"
                     /locus_tag="J8I47_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAZ6321801.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4320..4687
                     /gene="rnpB"
                     /locus_tag="J8I47_000335"
     ncRNA           4320..4687
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J8I47_000335"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4896..6062
                     /locus_tag="J8I47_000336"
     CDS             4896..6062
                     /locus_tag="J8I47_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAZ6321802.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6108..7604
                     /locus_tag="J8I47_000337"
     CDS             6108..7604
                     /locus_tag="J8I47_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAZ6321803.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7685..8368
                     /locus_tag="J8I47_000338"
     CDS             7685..8368
                     /locus_tag="J8I47_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6321804.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9013..10203
                     /locus_tag="J8I47_000339"
     CDS             9013..10203
                     /locus_tag="J8I47_000339"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAZ6321805.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10305..11258
                     /locus_tag="J8I47_000340"
     CDS             10305..11258
                     /locus_tag="J8I47_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAZ6321806.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11327..12676
                     /locus_tag="J8I47_000341"
     CDS             11327..12676
                     /locus_tag="J8I47_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6321807.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12859..13182)
                     /locus_tag="J8I47_000342"
     CDS             complement(12859..13182)
                     /locus_tag="J8I47_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321808.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13311..14474
                     /locus_tag="J8I47_000343"
     CDS             13311..14474
                     /locus_tag="J8I47_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321809.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14544..15893
                     /locus_tag="J8I47_000344"
     CDS             14544..15893
                     /locus_tag="J8I47_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ6321810.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15938..16369)
                     /locus_tag="J8I47_000345"
     CDS             complement(15938..16369)
                     /locus_tag="J8I47_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6321811.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16757..19399
                     /gene="alaS"
                     /locus_tag="J8I47_000346"
     CDS             16757..19399
                     /gene="alaS"
                     /locus_tag="J8I47_000346"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAZ6321812.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19599..20195
                     /locus_tag="J8I47_000347"
     CDS             19599..20195
                     /locus_tag="J8I47_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ6321813.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20317..21465
                     /locus_tag="J8I47_000348"
     CDS             20317..21465
                     /locus_tag="J8I47_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAZ6321814.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21503..21916)
                     /locus_tag="J8I47_000349"
     CDS             complement(21503..21916)
                     /locus_tag="J8I47_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAZ6321815.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21995..22426
                     /locus_tag="J8I47_000350"
     CDS             21995..22426
                     /locus_tag="J8I47_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAZ6321816.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22525..22758)
                     /locus_tag="J8I47_000351"
     CDS             complement(22525..22758)
                     /locus_tag="J8I47_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAZ6321817.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22941..24611
                     /locus_tag="J8I47_000352"
     CDS             22941..24611
                     /locus_tag="J8I47_000352"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAZ6321818.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24758..25144
                     /locus_tag="J8I47_000353"
     CDS             24758..25144
                     /locus_tag="J8I47_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321819.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25131..25829
                     /locus_tag="J8I47_000354"
     CDS             25131..25829
                     /locus_tag="J8I47_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ6321820.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25834..26298
                     /locus_tag="J8I47_000355"
     CDS             25834..26298
                     /locus_tag="J8I47_000355"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAZ6321821.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26295..27206
                     /locus_tag="J8I47_000356"
     CDS             26295..27206
                     /locus_tag="J8I47_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ6321822.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27441..27980
                     /gene="pyrR"
                     /locus_tag="J8I47_000357"
     CDS             27441..27980
                     /gene="pyrR"
                     /locus_tag="J8I47_000357"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAZ6321823.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27994..29277
                     /locus_tag="J8I47_000358"
     CDS             27994..29277
                     /locus_tag="J8I47_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAZ6321824.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29290..30216
                     /locus_tag="J8I47_000359"
     CDS             29290..30216
                     /locus_tag="J8I47_000359"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAZ6321825.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30235..31515
                     /locus_tag="J8I47_000360"
     CDS             30235..31515
                     /locus_tag="J8I47_000360"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAZ6321826.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31520..32596
                     /gene="carA"
                     /locus_tag="J8I47_000361"
     CDS             31520..32596
                     /gene="carA"
                     /locus_tag="J8I47_000361"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAZ6321827.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32601..35786
                     /gene="carB"
                     /locus_tag="J8I47_000362"
     CDS             32601..35786
                     /gene="carB"
                     /locus_tag="J8I47_000362"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAZ6321828.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35840..36616
                     /locus_tag="J8I47_000363"
     CDS             35840..36616
                     /locus_tag="J8I47_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAZ6321829.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36613..37545
                     /locus_tag="J8I47_000364"
     CDS             36613..37545
                     /locus_tag="J8I47_000364"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAZ6321830.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37542..38258
                     /gene="pyrF"
                     /locus_tag="J8I47_000365"
     CDS             37542..38258
                     /gene="pyrF"
                     /locus_tag="J8I47_000365"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAZ6321831.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38265..38954
                     /locus_tag="J8I47_000366"
     CDS             38265..38954
                     /locus_tag="J8I47_000366"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAZ6321832.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38927..39196
                     /locus_tag="J8I47_000367"
     CDS             38927..39196
                     /locus_tag="J8I47_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321833.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39234..39875
                     /locus_tag="J8I47_000368"
     CDS             39234..39875
                     /locus_tag="J8I47_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6321834.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39906..40610
                     /locus_tag="J8I47_000369"
     CDS             39906..40610
                     /locus_tag="J8I47_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAZ6321835.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40607..41509
                     /locus_tag="J8I47_000370"
     CDS             40607..41509
                     /locus_tag="J8I47_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6321836.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41667..43373)
                     /locus_tag="J8I47_000371"
     CDS             complement(41667..43373)
                     /locus_tag="J8I47_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAZ6321837.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13930 a         7684 c         9466 g        12449 t
ORIGIN      
        1 aaaatggatt ttctaatttt taagctaaat gttagatcac acatgtatat gctggatagt
       61 cttataaaaa atcatgtaat acagcttggt acatgatttt tatttgtagc tgttttttac
      121 atagacacaa aaaagaaacc acgcttgtag acgtggtttc ttttttacta tctgtttcca
      181 gaagctgagg tgctgctgga tggtgtatgg tcatttgcag catccgctcc gctagttgct
      241 ggctcactgg aagaagcagg aggagcagat gaagacggtg tcgagctttc ttcggatgaa
      301 gaagaaggaa ccgttgttga agacgctggc tcgcttgttt ctgctgtcgt gctttctttt
      361 gaggactcag cagttgattc gctttgacta gtagagctag agctttgagt cgttgtctct
      421 gttgttgagc ttccaggagt ctggacatag ctggaagatg gaatagttgt tgatggtgta
      481 attgcattcg atctagctgt atattgatct ttgtagtaca gttcaccgcc cacacgaatt
      541 aatccgcttg gcatctccca gtctgtattt gtcacgttag ctgaaacata ctgcataagc
      601 tcgcgataaa catcagaggc tacgtgtgac gattctgaag tgacaggtgt cattttttta
      661 ttgtaacctg tccacacgga aatcgaataa tttggcgtat agcccgcaaa taggatatct
      721 ggatacaccc cagaagaaat accaagtttt gcatactcgt catctgtgta attggatgtt
      781 ccggtcttac cggcttgata taacccagcg atttgcgcat tcgtaccagt tccttcagtg
      841 atcgtatctt ttaaaatgtc tgtgatcata taagctgttt ctggagacat ggctgttttt
      901 ccatctggct cataagtttc ttctgttccg tcttggaaga cgattttatt aacatattga
      961 ggtttgtaat aagtccctcc attggcgaaa gccgcatagg ctgctgccat ttttaaggaa
     1021 gaagctccgt attttgtccc atcttgttct tccgtattac tagagatcgc attcgattga
     1081 tggatcgtac tgtattcgat cccaagattt tttaagaaag aagccacttt atctgaaccg
     1141 acttcattga ataatttgac tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa
     1201 gtaatggtgc ccatatattg attatcccag tttcctacag gagtcgaagt cccttcatag
     1261 ttatacggtg cgtcagtaat agtttttcca gtagaatatt tcaaatattc aaaagctggt
     1321 ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac gggaagtatt cactgctaag
     1381 ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc caatctgggc ttttactttc
     1441 ccggtattgg tgtcgataag ggtggaggca acttgcatct cgtcgtctgg gtaagagaca
     1501 tactgatccg tgttgacgat atcatatagt ttcttttggg cgtccagatc caaattcgta
     1561 taaatctcta aaccgtctgt gtagacattc ttatctgttt tttcttgtac ttcattgatg
     1621 acttctttga cataattatc gacgatctta gtattgtcat cggattgagt tagttcttgc
     1681 agaccatccg ttaccggtac attaacagct tgatcatatt ctgtttgaga gattttttcg
     1741 ttttgaagca tcgtatacaa aacagtatct cgtcttttct tcgcctgatc tggataaaca
     1801 taaggatcat aggcggaagg ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa
     1861 gaaagttctg ataatttctt accaaagtac atctcagaag ctgtctccat cccgtacaag
     1921 ccgtttgaca tatacacttt atttacgtag taggtcaaga tctcttgttt cgattttttt
     1981 tgttccaatc ttacagccat ccacgcttct tgggccttgc gtttcagagt ctgatcctcg
     2041 gcactggtgg agaagaagga aagtttgatc aattgctgtg tcaaagtact tccgccttgc
     2101 agtccgccag aggtaaagtt agagagggca gaaccgatga tccgaatagg gtcaaccccg
     2161 atgtgtttat aaaaacggcg atcttctacc gacacgatcg catcttctaa tgttttcggt
     2221 aattcattcg cagaaatttt ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca
     2281 tcttgtgtat atagtttgga agatacggta gcgtctaatt ttttatccgt caattcgggc
     2341 gcatcttttg cgtaatacca gaaaagtcct acacctgcta aaaagaggat acagaaaaat
     2401 gagagtattc ctaaaaggat cttgataaat aatccttttt tatgtgttcc agaggacttt
     2461 ccagaacctt tgccagaatt cttcttcaca ctctttttag gagtaggctg tttctgtcgt
     2521 ctagagcttc tagtttgttc atttgccatt cttgctttct ccttcaatag ataatgttga
     2581 taaatagaag tcaaccgcgt ccaaataagg aatccgcgga gaaataccag ttttgatctc
     2641 tattcctagt tcttcgatca aagataaagg tagtgatttg cgtccttttt ccttttgggc
     2701 gtcccagtaa gcaatcaaat gaacactatt caaataaaaa cagcgcttca aagacgaaaa
     2761 ccataacaag acaaagcaga ccccattttg ttcgaggcat gctttcatat gttcgatttg
     2821 atgggcatgg aagtttttaa agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa
     2881 gtccaagtaa aagcctcgat agacgccatt atagtctgta gttgaggctt gggtaaagta
     2941 ggcttccttg atcacagcag cacttctctt aggatagtct actttcacga tttggatagg
     3001 ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga taatatttat tactttcatt
     3061 gattgcttct tcaaagcgca tcccccgatt gccgaattca gtgatctgat ttcttttttc
     3121 ttgaattttt tcgacgggag aggcattctt ataataaggt tggccatttg gatagcgcaa
     3181 taccatcttt aatcacactc ctaggccaca ttatagcaaa aaagcggata gaaagaaaag
     3241 gaatctgaga tggataagtc aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc
     3301 ggtgttttta aagaaaatga tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa
     3361 ctccatcagt tagcagaaga agggctggaa tgggtacttg tcagtgggaa tttgggcgtc
     3421 gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag aatatccgga actatctctt
     3481 ggtattttgt atcctttttc tgattttggt aataactgga atgaagggaa ccaagaaaaa
     3541 aaacaattag cagaacaatt agcagattat gtcgaagctg ttagccatca accctaccaa
     3601 tcacctagtc agctaaagaa tcacactcgt tttatacttg agcatacagt gggctgtctg
     3661 ctgatctatg atgaagaatt ccctggaaaa acgaagtatt tttttgaaga tgcacagaat
     3721 taccaagcag atcatcccta cgaaattcgg ctgatcagta tggacgatct tcaaaatttt
     3781 agtgaataat cgatattgtg tttgatttat caactttttt cctatacaat gaaaaaggta
     3841 gaaatgttat tacgtaaatg aaaactagag gtgtaaatat ggcgaatttg gtttatagtc
     3901 ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg cggctatgat cccgttgaag
     3961 ttgatgaatt cttagacaat atcatcaaag attatgaagc ttacagcaaa gaactccttg
     4021 ctttacaaga agaaaacgat cgattgagtg caaaagtagc tcaattatct aaaactcaag
     4081 gagcagctca aacacgcgtg caacaaacag aggcaccaaa gagcgcagca gtgaccaatt
     4141 ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc
     4201 aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa taactacaca aatgcagaca
     4261 caagcttaga tgataatgaa aaaacacgtc aattttaatt tttgattaat gaatttgcgg
     4321 ttttcgggta atcgcggtct gacttgatca ggctgaggaa agtccatgct cgcacaggct
     4381 gtgatgcctg tagtgttcgt gcttagcgaa accataagct aaggtactct ttggagtaac
     4441 ggcaggaaaa caagctaagg cttcggctat gcttaagtat ccttgaaagt gccacagtga
     4501 cgaagcggct ggggaaaccc atccggtgga acgcggtaaa cccctcgagc gagcaaccca
     4561 aacaatggta ggggcgcttt ttgtctggaa gaagaacagg acaaaaaggc agtataactg
     4621 cagatagatg gttaccgctt tgttttttct ccctggaaaa gcaaagtaca gaacatggct
     4681 tatagaaaac cgcaaacatc agggaattag gtcgtgacaa aagtaatgcg ttataagaaa
     4741 caagaaaaac atttgaaagc tatttttcaa tacttcagat gaatcgttct tattacaaaa
     4801 caaacttttg tatcaccggt tattcggaat ttgagtaaaa gtgtggggta ggactgatgt
     4861 cccacctcct ttttttataa ataaggagat tgtgtatgga aaaaaacagg aagttcaacc
     4921 tcgtcgcaac tgcggcaagc gggctagaag cactggttgg taaagaatta agagatcttg
     4981 gaatcgaatg ccaagttgag aacggacgtg cacgttttga aggaacaatg gaaacgatcg
     5041 ctacagctaa tttatggtta cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg
     5101 atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc attgccttgg gaagattttc
     5161 ttccactaga tgcagctttt cctgtagctg gaaaatcgat caagtcgaaa ctatatagca
     5221 cacctgattg ccaagcgatc acaaaaaaag caatcgttga acgtctccgt acctattatc
     5281 accgtccagc ttctgtacca ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt
     5341 tgctgaaaga ccatgtgatg gtaactctgg atacgacagg accaagttta ttcaaacgtg
     5401 gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa catggcagca gcacttatta
     5461 tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc
     5521 tttgtattga agcagcctta ataggccata atatcgcacc aggattcaat cgtgagtttg
     5581 cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga aaatgttcga tcagcagccg
     5641 aagaaaaagc agattatgat attgaattag atattactgg ttcagatatc aatggacgga
     5701 tgattgaaat tgccaaagcc aatgcagaag aaatcggatt aggctcttcg attaccttta
     5761 agcaacaagc agtcaaagac ttcaaaacgg ataaagaata cggggtcatc gtggctaatc
     5821 cgccttacgg agaacgtcta ggagaagaag aaactgttcg gaaattatat aaagaaatgg
     5881 gcgatgtatt ccgtccatta aaaacatgga gtaaatatat cttgactagc gacttagcgt
     5941 ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc
     6001 gtacggatct attccagtat tggggaactc gtccgcctag aaaaaaagag aaagacgaat
     6061 aatcttacta attgattcat tcaattggat gtttggagga atagggaatg aaagaaaaag
     6121 agtttttaca agaagtaaaa gaaatcaatc ttctacaaca agctttagga atcctcgatt
     6181 gggatacaca aacaggtatg cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct
     6241 atctttacag catttatttt tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct
     6301 atttttctga acatccggaa gaattgtcgg aagttggaac tgccgttttt gccaaagtaa
     6361 aagaagagta tgatttggaa catcgcgtac cggaagatct tatgagaaaa tatacagctg
     6421 ccgtttcttc agcacacgga caatggcaaa aagctagaga aacacaacaa ttttctgatt
     6481 ttcaacacgc attgaatcaa aatattgaat taacaaaaca gttgatacct tactggagaa
     6541 aagatgaaaa gacgaattat gatgttttgc tgaatcaata cgaacccggg atgactgtcg
     6601 aaatccttga taaggtattt gatcagctta aagaaggaat tctttcgatc cgccgtacgc
     6661 tccaagaaaa agggactgaa cctgaaacag actttttaaa tcggagaatg acgaaaaaac
     6721 aacagaagag atttgtagtg aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc
     6781 gtttagatga cactgtccac ccatttatga ctggaattaa tccgaatgat gcacgaatca
     6841 cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt tggagtgatc cacgaggctg
     6901 gtcatggtat gtatgaacaa gacatcgatg ataaatatgc gtatacaccg gtttatgaag
     6961 gtacatcaat gggtatccat gaatcacaat cgttgtttaa cgagatcatt attggaagta
     7021 gtcgcagctt ctggaaaaag caatatcctt tctttcaaga atgtgcagaa ggaacattcg
     7081 atgatattcc ttttgacaaa ttttatcgtt cattgaacca tacaaaatca agtttggttc
     7141 gtatcgaggc agacagtttg acttaccctc tccatatcat cattcgttat gaaatcgaaa
     7201 aaatgatttt caatgaaggg gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat
     7261 atgaagagta tctgggaata cgaccaacaa atgatgtgga aggcatccta caagacgttc
     7321 attggtcagg tgcaagcttt ggttattttc cctcttatgc attagggtac atgtatgcgg
     7381 cacaattact tcattctttg cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg
     7441 attattctcc aatcaaaaac tggatgtcag agcatatcca tcaatatggt gcatctagaa
     7501 aaccaaatca attaatcatg gatgcaacac atgaaccgct taatccgcaa tacctgatcg
     7561 actatatgaa agacctgtat tttaatgtat atcaggtaaa ataaaataca atttacaaag
     7621 atagctgaaa ttcatctaat ctatagatgt tttcagctat ttacttaaga aagaagtgga
     7681 acgaatggat aaattacaag tgatcgctga ttatagtatg gaccaattag cgcatgatca
     7741 aacaggacat gggagcgatc ataccaaaag agttgtcaag ttagctgaaa aaattttgga
     7801 gacggaaccg caggcagacc gctttgtgac gcttgcagca gcttacctac atgatacgat
     7861 tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa cagcttcgtg tattccttcg
     7921 ctctttgcca atagtagagg aacagatctc gatgattttt tccattatcg aaaatatgtc
     7981 cttttctaaa aatctctctg aagcggccga actatctctg gaaggtaaga tcgttcaaga
     8041 cgctgatcgt atcgaagcat taggagcaat cggcattttg cgtactgctt atttcggcgg
     8101 tggacatggg cacccgattt ttgatcctga actgtatccg caaacattca aggacaaaga
     8161 aaattaccga aaaggcacga cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc
     8221 tgataaaatg aatactgatt acggatatga agaagccaaa cgaagagaaa ccttcatgcg
     8281 tgaattctta gaagaatttt ttctggaatg gtctattgac gatgaaaatt ttactccaga
     8341 caattggatc aaaaataaca aaaaatgatg gaggttgtga cagaagtggt cagctccaag
     8401 atatttcgga tcattttccg taaaagttac ttctggaaca ctgtttacca agaggtcgtg
     8461 agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg
     8521 atgaatattg taacttgtga ttcggaattg gcaggagaac accgtttatt cggaagaata
     8581 ggactgtgac aagacttttg tcacagtcct tttttcgaat tcaacaagta aaacttttaa
     8641 gaaatattca gagagtaaaa ttaaaaattg ctgataaacg aagatggaaa ttttatgtat
     8701 ttttattggt ctatgcaata aataaattaa atattcagaa tacaattgat tttaacgaaa
     8761 gggcttctat caatggttgt gttgtattta tctgcccagg tagagtaaaa tagatcatta
     8821 tctaaaaggt aattgttcat actatataag tcatttgaaa gtaaaaagat actgtaaata
     8881 aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt tatcaaaaat atagcatact
     8941 tgctttgtag atgaatgaca cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg
     9001 gaggtggaga caatgtcttc caaagaacta acaatgtatt tagagaaaga attggatcaa
     9061 ctgaaagaaa aggggttata caatacgatc gacatactag aaagtgaaaa tggtgcttgt
     9121 atcatcgtag acggaaaaaa gatgatcaat ctagcatcca acaattatct tggatttgcc
     9181 aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg aaacttatgg tgtaggagca
     9241 ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc agcaattaga agaaaaaatc
     9301 gcagaattca aagggacaga agcagcaatc gcctttcaat ctggattcaa ctgcaatatg
     9361 ggcgctatct ctgcagtaat gacaaaagaa gatgctatct tatcggatga attaaaccat
     9421 gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa
     9481 gatatgaaag accttgagaa aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa
     9541 atcatgtata tcacagatgg tgttttctcc atggatggag acattgcacg attgccggaa
     9601 attattccga ttgttgaaaa atatggactg atcacctacg tcgatgatgc gcacggctca
     9661 ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg gattaagcga caaaatcgat
     9721 atgcaaatgg gcacgttatc gaaagctata ggtgttgttg ggggttatgt agctggatcg
     9781 aaaacactaa tcgactggtt gaaagctaga agtcggccat tcctattttc tacttcgcta
     9841 actccaggtg cagctgcagc tgcattggct tcgattactt tgatgcaaga acatccggaa
     9901 cttgtcgaaa aagtttggga aaatgctaat tattttaaag aagaattgaa aaaagtaggc
     9961 tataacatcg gtatgtctga aacacctatt actccagtca ttttgggaga cgaaaaagtg
    10021 actcagactt tctctaagaa gctgatcgaa catgggattt atgctaaacc aatcgtttat
    10081 ccaactgtgc ctcttgggac aggcaggatc agaaatatgc cgactgcgga acatacgagg
    10141 gaaatgcttg atgaagcagt agcagtttat caaaaaatag gaaaagaaat ggaaattatt
    10201 taactaaatt tagttttttc gtctatttaa aattagagaa catgttcacg catatattgt
    10261 taatcatctg aagatagtag taaaaaagat cagggaggat catcatgaaa aaagtattgg
    10321 tgacaggatg tttaggacaa attggttcag agttgatcac acggctacgt caagaactag
    10381 gcagtgaaaa tgtcattgcg acagatatac gcatgcctga agtcattgag aaaaacgata
    10441 tctttgagat cctagatgta atggattacg aaaaaatgcg cagcatagca gaacaatatc
    10501 aagtggacac attgattcat ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac
    10561 aattagcctg gaatttgaac gtgactgggc tggtcaatgc attggaagtc gctagagaat
    10621 tccagctgaa attttttacg ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag
    10681 atcatacccc acaagatact cttcagcgtc ctacaaccat gtatggggta acgaaggtag
    10741 ctggagaact actatgtgat tattattatg tgaaatatgg cgtagatact cgtggtgtcc
    10801 gtttccctgg attgatttct tatgcaacct tgccaggagg cggtacgaca gactacgcag
    10861 tcgatattta ttattcagca atcaaagaag gaaagtacac tagtttcatt gatcaaggta
    10921 catcaatgga tatgatgtat atgccagatg cgatcgatgc cattataaaa ctcatgaatg
    10981 cagatcctga tcgattggtt catcgaaatg ccttcaatat atcggcgatg tcttttgacc
    11041 cggaagagat caaagcatct attcaaaaaa tcataccaga ttttgaaatg gattatgatg
    11101 tggatcctgt acgtcagtca atagcagaat cttggccaaa ttcactagat gtttcttgtg
    11161 cgcaagagga atggggcttt tcgccgaaat acgacttaga tgtgatgaca aaggatatgt
    11221 tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa caagaaccac atccaaaaag
    11281 ttttttatac ttttgggatt ggctatggta tgaaagggga caagggatgg aagaatatca
    11341 agccacgcca gtaaaagaag taaaaataga aaatgaatta aaacgtacga tgggattttt
    11401 tactgcttta tcgactgtta tgggcacagt gatcggtgca ggggtatttt ttaaagccgc
    11461 aagcgtagca gaagttacag gatccgccag tttgcatatg ttttcttggt ttctcggtgg
    11521 tatgatttct gtctgtgcag gactaacagg agctgaactg gcagctgcta ttccagaaac
    11581 aggagggatg attaaatata tcgaacggat ttacggaaat acagcagctt ttttattggg
    11641 ctgggcgcaa gtcgtgattt attttccagc aaatgtagca gccttgtcga tcattttcgg
    11701 tacacagttc gtcaatctat ttggtctttc ccagtcaatg attgttcccg tagctgttac
    11761 agctgctgta tcgatcctgt tgatcaattt tttaggttct aaagctggag gggcttttca
    11821 gtcgattacg cttgtctgca agcttattcc tttgtttgtg atcgtcattt tcggtttgtt
    11881 ccgacaagaa ggagtagact tccaactgtt tccgatccaa gcaggagaaa atctttcatt
    11941 tttctcagcc ttgggagcgg gcttgctagc aacgatgttc gcatacgacg gttggataca
    12001 cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc
    12061 tttaggaatc atcggaatca tgatcgttta tttattagta aatgcggtgt tcttaagaac
    12121 cgcatctatc gacggagtcg caggaaacag caatgcggca agtgacgttg caaaaatgat
    12181 ttttggcggc ttcggtggaa gactggtcac agttggtatc ttgatttctg tttatggaac
    12241 catcaatggc tataccctta ctggaatgcg tctcccttat gttatggcaa aagaaaacaa
    12301 cctgcctttt agcaagctct ttgctaaact tcatgataaa actaaagttc ctgtagccgc
    12361 aggtatctta gagctagtca tcgctattgg catgatgatg atcggtggtt ttgatacact
    12421 aacggatatg ctgatctttg tcatttggat tttttatacg atggtctttg ttggtgtcat
    12481 tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt
    12541 tattccacta gtagcaatca ttggagggac ttttatcgta tcaagcacgc ttatcactca
    12601 gaccttttta gcttcgatgg ggatagtatt gacacttgct ggtatcccga tttatctcta
    12661 tttaaaacgt agatagctaa aacattcgaa ttttattaga actgcacttc tctgccaaac
    12721 aaaatatttt atttaaatga ggagtacgtg gaagaaatgt caaaagtact cgttaatttt
    12781 gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc
    12841 tcaaccgatt tctgtctttt actttgattt gcgtaactga actattcggc gtacgccgcg
    12901 ataaatattt aataaaacca agcaaccaga aatgaaaatg ggaagccaaa tcagatagac
    12961 agggtagcca agcactttag acaaagcatg tggagtcatt ttatccattt caagaagtgc
    13021 ttgctgccaa gtctgaagat tgaagactag catgaaaccc gaaataatca ggagaataaa
    13081 tcctaaataa tgaattgtag ttctttttct tacataagcg atcgtagcat aaaggaacag
    13141 taaaaaaata ggtacgccaa aaacaaagta catattcagc atctcgacac cccctttgtg
    13201 tttatcttac tttactttga aagaaatgag aaataatagc ctcgttactt gcttgaaaca
    13261 gcaaaggact gctaagataa gagagataca aagaatcaag gaggagtcat atggcaagtt
    13321 atatcgaacc gattcgtttt gcgctgattg tttttccatt tttagcctta gcgatctcat
    13381 ctgttttttt tatttatgaa tatcgaaagt atggaacatt tctagtaaca agggcagtta
    13441 ttttgtattc tttcgttttt tatttattgt gtgcgtattt tttggtgatc ttaccacttc
    13501 cggcaaagga gacggttgcc caaatgacag ggccaaagat ggagctgcat ttatttgctt
    13561 catggcaaca tttcagagac aacacggtat tggtcttgtc tgaacctagt acctacctgc
    13621 cggcaatgaa gcagagtgta tttttggaac cagcttttaa tgttttatta ctcataccat
    13681 ttggcattta tttaagatat tatttccggc tttcttttat gaaaacgatg attgctagtt
    13741 tctgcctttc tctatttttt gaattgacgc agctaagtgg actttatttt atctacccaa
    13801 gaccataccg cttatttgat gtgaatgatt tagtgacaaa tacattgggt ggaatcattg
    13861 gttttgtcgt tacgccgtta tttacattta tgcttcctac aagaagacga atggatgaac
    13921 tttcttatga aaaaggaaag actgtctcat tgactcgacg cttggtcgct tatttgatcg
    13981 actggacatt tttatctctt attaatttgg tagcggcgat tttctttaaa attgcgttcg
    14041 attcaggggc agttacttct catatctggt ggcttttatt agaagtaggg gtatacttta
    14101 tgattgtacc gtttataacc aatggatgga cacttggcaa aaaaattgtg cgtatccgtt
    14161 tggtggaaga agaccgcgaa cgaatttctt taaaagcatt gatgatccgc tacgggtatc
    14221 tgtactttct tttttatggt ttaagctggt tggggcagtt gcttcagcaa atcatgaatt
    14281 cgacaacgaa tacggtgttg cagatgagtg cagtattcat ttctctcggt gtcttgttac
    14341 tacaaggtat ttttatttta aatatcttgc tatcattttt gagaaaaaaa cggattctgt
    14401 tttatgaaaa agctagtcac acatatactg ttagcacgat tggccaaaca aaggatgatg
    14461 attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata
    14521 atttaaagag aagaggtgca cgaatgaata attttgatat attccgcttc aaaccgttta
    14581 tcaatgaagc gttaaccgca aagggcttca aacagcccac agaggtccag gaacgtttga
    14641 ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa
    14701 cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag
    14761 ttgttattac agctccaagt agggaactag ctgcacagat ctatgaagca gctacacaaa
    14821 tcgctaaatt ttctacaccg gagatccgtg tctcgaattt tgtcggcggt acagacaaac
    14881 agcgtcaatt aaatcgtctg catcaccaac agccacaggt tgtagtaggc acgcctggta
    14941 gaatattaga catgatgaat gaacaagcgc tgaagattca caccgcattt gcatttgttg
    15001 tcgatgaagc ggatatgaca ttggatatgg gttttttaaa tgaagtagac caaattgcaa
    15061 gtcgattacc agaaaagctg cagtttttag ttttttctgc cactattcca gaaaaacttc
    15121 gtccattctt acgaaaatac atggaaaatc caatcattga agaaatcaaa ccaaaagcag
    15181 ttatttctga agacattgaa aattggctga tttcaacaaa aggtaaagat gagaatcaaa
    15241 tgatttatca actgctgaca attggtcatc cttatttagc aatcgtattt gccaatacaa
    15301 aacagcatgt ggatgaaatt gccgattact tgaaaaacca aggcttgaaa gtagcaaaga
    15361 ttcatggaga catcacccca agagagagaa aacgagtgat gcgacaagta cagaatctgg
    15421 agtatcaata tgttgtagcg actgatttag ctgctagagg gatcgatatc gaaggtgtct
    15481 ctcatgttat taatgcagaa attccagaag atctagactt ctttatccat cgtgtcggac
    15541 gtactggacg taatggctta aaaggaacgg ccattacgtt atattcgcca aatgatgagc
    15601 aagccattga tgcgatagaa aaattgggcg tctcatttga accaaaagaa atcaaaaacg
    15661 gggaaatcgt caaaacatac gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg
    15721 tattagatcc cacgctgatt ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt
    15781 ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac
    15841 gcagacagca aagccgtact gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa
    15901 gaggttgtga caaaaggcgt gtcacaatct cttgttttta agctagactt tttttcattc
    15961 cgatacaaag tacttcatct tcaataaaag gttctgtgca agtatgatac ccttgttttt
    16021 cataaaaatc tacagccgtc atttcagcta ctacataact agtctgacaa ccttctgaaa
    16081 ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa cccttttcct cggtattctt
    16141 ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt tttcttttca taacgcaaag
    16201 tagctactgg tcttttttga tcaagaaata gataataggt ttgttgcggg atatcttttc
    16261 cgtcgaattc tagttctgca ggaatccctt gttctaatac aaaaacttga tatctcaaaa
    16321 agcagctagc tgcgcgtatc cattcatccg ctccaactat ttttttcatc atattcctcc
    16381 ttgtcatgaa tagcagagat gtattgattc ctttattctt atcgtaccaa aaatcgtgga
    16441 gtatgaccaa aaggaaatga aaataaaaat tgagatattg acagtgatca atagaatgcg
    16501 tatactaatt aagtacgaaa aggatatgat ttgtttttta agactcacag aaaattcttt
    16561 cttggctgaa aaaagaatag tcactaatca aattgctccc tttttctatt ttggtagaaa
    16621 taccgacgca gctcagcgtt atcgagttta agaggtaatg attacacatc attgcaatca
    16681 aggtggtacc gcgagttttc gtccttggct gtgatggtgt ttttttgtat ttaatttata
    16741 gaaagcaggt aatgacatga aacaattaag tagtgcacaa gttcgacaaa tgtttcttga
    16801 tttcttccaa tctaaaggac atacgattga acctagtgcg tctttgatcc ccgtgaacga
    16861 tccaacctta ttgtggatca actctggtgt tgcaacattg aaaaaatatt ttgatggttc
    16921 agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat
    16981 cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt gagatgctgg gtaatttttc
    17041 gattggtgac tatttcaaaa atgaagccat ccactgggca tgggaatttt tgactgatcc
    17101 aaaatggatg gcattcgatc cagaaaaact ttatgtaaca gtttatccaa aagatacaga
    17161 agcaaaacgc atctggcatg aagaagttgg cctgccacta gatcacatta ttgatgtaga
    17221 agacaacttc tgggatatcg gtgcaggccc atgtggtccg gatacggaaa ttttctatga
    17281 ccgtggagaa gcttataacg atttagcaga agacgatcct gaaaactatc caggaggaga
    17341 aaatgaacgt tatttagaaa tctggaactt agtattttca gaattcaacc ataagcctga
    17401 taacacattt gaaccacttc ctcataaaaa cattgatacg gggatgggat tggaacgcat
    17461 ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca gacttgttta tgcctattat
    17521 ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt gaaaacccag taacagatat
    17581 ttcttacaaa gtaatcgcag atcatattcg cgccctttca tttgctattg gcgacggtgc
    17641 attgccatcg aatgaaggga gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat
    17701 gcatgggaaa aaattaggta ttgaacaagc attcttatat cgtttagtcc ctgttgttgg
    17761 ggaaatcatg gtaagttatt atcctgaagt acttcaacaa aaagaattta ttgaaaaagt
    17821 tatccgtaca gaagaagaac gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa
    17881 tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact ctagcaggaa aagacatctt
    17941 caaactatac gacacatatg gcttcccggt agaattaacg gaagaggtgg cagaagatga
    18001 aggactgaaa gttgatcatg ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc
    18061 tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa tctgctttat tgacagatat
    18121 caaagtagaa agtaaattca ttggatacac agaacaagaa tcagatagtc aattattggt
    18181 catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt
    18241 tgatcaaaca cctttctatg cagaaatggg tgggcaagta gcggatcaag gatggatcat
    18301 gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt caaaaagcgc ctaatggtca
    18361 atttatgcac accgtagaag taaaagcacc aatgagtgaa ggacaaacct atcatctatt
    18421 gatcgatcaa tcgatgcgca atcggatcat caaaaaccat acagcaaccc atctactgca
    18481 caaggcattg aaagaagtac ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc
    18541 tggacattta cgattcgact ttacccactt tggtcaaatc acttcagatg aattagctga
    18601 aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc ccagttgaaa caattgaaac
    18661 agatatcgat acagccaaaa acatgggtgc aatggctttg tttggtgaaa aatacggaca
    18721 tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt
    18781 agcaaataca gaaaacatcg gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg
    18841 cgtgcgacga atcgaggctg taacaagtaa agaagcgtac gaactacttg aagaagaaga
    18901 aaaacaacta aaacaagtag caacaattgt taaatcacct caattgaaag aagttgtttc
    18961 aaaagcggaa caattgcaac agcaagtaag agagctacaa aaggaaaatg aacaattagc
    19021 tagcaagtta gcgaatcaac aagctggtga tgtattcaaa gatccagaaa cgatcaatgg
    19081 aaaaacaatc attgcagcac aagtaaaagt gaaagatatg aatcagctgc gtcaattagc
    19141 tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt ttagcaaacg aacaagaagg
    19201 aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat gaagctggac taaaagctgg
    19261 cgacttgatt aaagcaatcg caccgaaagt aggcggtggc ggtggtggac gtccagacat
    19321 ggcacaagct ggcgggaaaa atcctgctgg tattccagat gcacttaaag ctgcgaaaga
    19381 atgggttgcg caacaatagg gcgtttacgt ttgacagaaa aacaaaagtt caaacattga
    19441 acgaaacatg attatggggg cgaagacaaa caagtcttgc cctcatactt ttttcaattg
    19501 gatggcgcat ctaatcaaat ggtcggattc ggatggtctt tagttgacga aagtgacaga
    19561 aatttttacg attagtataa ataaaaaggg gtgaaaaaat gaatagtaga aataagacct
    19621 ttcgactagt cttacgtgcg atcttgttag caatcatcat cgtacaagcg atggttcctt
    19681 ggctggggtt tattccatta ggattcatta gtttaacaat tatccatatc acagtaatta
    19741 ttgctgcagt agtattaggc ccaaaagatg gcatggtgat tggcttgttt tggggaatcg
    19801 ctacaattgt tcgtgcctat gccatgccaa caacgccatt tgatacatta gtgttcacga
    19861 atccgattat ctcggtagtt cccagagtac ttgttggctt agtagcaggc ttggttttcc
    19921 actggattta tcaaaaaaat aaatcaataa cgatcagctc agcctttgca ggggtactgg
    19981 gttcattggt caatacagtc ttagtgttag gattcatggg attattttat acaggtgcaa
    20041 cggcagatgc ctatggtgta gactctgctc ttttatttaa aaccttagca ggggttgcag
    20101 caatcaatgg gataccagaa gcaataggtg cgggaatcat aacaccatta ctagcaaaag
    20161 cgttatttgc agcaacaccg ttgaaaccag aataatgacc atcttttaat agtaatagga
    20221 cgcacgatag atagaaaaag gaagagagga gaggaaaaca atcgtcctct ccatcttttt
    20281 acctgagcaa caagcaaact agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa
    20341 aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac tagcgggtat cttaggattg
    20401 gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc
    20461 tccattatgg ctgatgcagt gaacagtcta tcagatactg cttcatccat attgactttg
    20521 atcggattta aaattgctgc aaagccagca gatcaggaac atccttatgg acacgaacgc
    20581 ttcgaatata tcagtgggtt atttgtttcg atcatcatta cttatgtagg atttcaattt
    20641 ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg tctctttaac acccatcgtc
    20701 ttttttgtcc taatcatttc catctttttg aaatttgcac aaggcaaaat gtatcaggtt
    20761 attgcgcaat ccattgattc tgaaacattg agagctacag caacagatag ttataatgat
    20821 gtctttacta cactggctgt attgctttca gcagcaatcg aatgggtaac tggatggcgt
    20881 attgatggat atataggttt tgctttagca ttatatatcc tttatagtgg attgacgatg
    20941 cttcgaaatt ttgtatacga actattagga agtcgcccaa cgccagcaga gattgaagaa
    21001 atggaagagc atttaaatgc ttgcgatgat atacttggct atcatgattt attggtgcat
    21061 aactatggac ccaataaacg ctttgcatca gtccacatcg aagtagatga atcaatgaat
    21121 ttgaatgtgg ctcataagat tattgatctg atagaaaaag actttaaaaa gtctttggat
    21181 gtggaacttg tctgtcactt ggatccaatg cctgttgata acgaaaaata ctataatatc
    21241 ttgcggcagt taaagcaaat tcttcttgaa acaggtacag ggcttaatat tcatgatttt
    21301 agagtaattc atcaaggaaa aacgctgcaa tttgatgtag tggtccctga gaaattccag
    21361 tttgttgata cagaactaga aagaatctta cgtagtcaaa tagaagaaaa aatcggagat
    21421 taccaattgg aaattacgtt tgatcacaat tatcttttat attaaatgag aaactataaa
    21481 aagactcctt aaagttttta atttaaagag tctttttttc ttttaattgt ttatggagag
    21541 cctgacgtgc aagattatct gctcctttat ttttgttttc gggcaaccat tgcaggatca
    21601 ataaagaaaa ttcggataga agttcttgga ttctttgaag gtagggaagg aagagctcgt
    21661 tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa
    21721 tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa agtttttaaa aagacttcga
    21781 actctgcttg atggtttgtc cctatagaaa gcgggataga tagctgttct tgtttacctt
    21841 gatagacaat caagattcca cctccactag gaccagggtt gccttttgta gaggcatcaa
    21901 tgtatacttt taacataaaa agagattctc ctttttgttc aaactaatgt tatggtaaga
    21961 ttatatcatt actgaatcgg tgagaggtta gagaatgaaa aaaaatattc gatggcagcc
    22021 tgaactggca catacgatca tctattggtc attgaccttg atcattctgt tttttagtct
    22081 gattctttct ttagaaaaca caggtcctta ttggaaaagc aattttgttt taggtatttt
    22141 tcttgttttt ctatttctag gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat
    22201 tatacgttat gctgcttttt ggaaagaccg agaaatatct gtcggacaga ttcgatcttt
    22261 gacgccaact aatactgggg tgcaaatgat gttgcaagat gagaaagccc catctgtttt
    22321 catgatgcga aaaaaagatc aagtagtcct ttttgatcat tttgaaaaaa acggacccaa
    22381 atcagaatca attccttatc cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga
    22441 acctaaaaaa acgaattttt acaggaaaag aaaagagtga gacgtacggt tcatcccgtt
    22501 tagcgtctca ctctatttgt ttatctattc atgcatacta gtatcggtag tctgaacgtt
    22561 gcctttcttg acatgggcag cttgtgttcc tcgattccct tcgatgatct caaattcgac
    22621 cacttgattt tcttccaaag ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa
    22681 aatttcttct gtgtcatcat aactgataaa gccgtatcct tttttgttgt caaaccattt
    22741 tacaattcct gtggtcaaag cactcacgac ctttctaaag tctgatcata gccttcatta
    22801 taaagagtat ttataagtac gtcaaataaa ttaaaaacct gtaattttat gttttttttc
    22861 tgaataaaaa aaggtataat tggcatatct taacaaagaa gatattttgt tgaataaagt
    22921 tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat ttcacaagaa acagagctag
    22981 aaccgattgt tgaagtggct agtaaattag atcttaagga agacgatttg gaactgtatg
    23041 ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg atcaaaggat aacgctgagg
    23101 ggcatttgat cttagttact tccatcaatc caacaccagc tggcgaagga aagtcaacga
    23161 tcactgtagg gttaggagac gcgctcaata aaatcaataa aaaatctgtt attgcattgc
    23221 gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg
    23281 cacaggtatt gcctatggaa gatatcaact tgcattttac tggtgacatg catgctatca
    23341 caacagctaa taatgcatta tctgctcttt tagataatca tattcatcaa ggaaatgaat
    23401 tgaacatcga tgcccgccga gtgatttgga aacgggtagt cgatctcaat gatcgtgaac
    23461 tgcgtaaagt aattgttggc ttgggtgggc caatccaagg tgttccaaga gaagatgggt
    23521 ttgatatcac agttgccagc gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag
    23581 atttaaaggc tcgtcttgca aggatcgttg tcggctatac atttgaccgt cagcctgtga
    23641 ctgctggaga cttgaaagca gaaggcgctc tggcattact gctgaaagat gcaatcaagc
    23701 cgaatcttgt acaaacaatt tacggaacac cagcttttgt ccacggggga ccgtttgcca
    23761 atatcgctca tggctgcaat agtattttag ctaccaagac agcattgaga ttagcagatt
    23821 acgttgtcac agaggcagga tttggtgcag atcttggcgg agagaaattt ttagatatca
    23881 aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat
    23941 tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa tgagaatctt gatgctttga
    24001 aagtaggttt tgcaaattta aagcgccata tacgtaatat ggaacaatat cagctacctg
    24061 tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa tgaactcaca ctgttggagc
    24121 acttgtgtga aaatcagggg attcttgcca agagagcgag tgtatgggca gatggagcag
    24181 aaggcggcgt tgatttagca aaagctgtag tacgattgat tgatagaaaa gaagctaatt
    24241 acaagccatt ataccgccta gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga
    24301 aaatttacgg aggaaatggt gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt
    24361 ttacgaagaa tggatgggac aaactgccga tttgtatggc aaaaacacag tattcatttt
    24421 ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac catcacgatc cgcgaaatca
    24481 tcccaaaatt aggggcagga tttttagtag ccttgactgg agatgttatg acgatgcctg
    24541 gtttgcctaa aaaacctgct gcattgaata tggacgtgac aaatgacgga gaagtgttgg
    24601 gattgtttta agcttgatta taatctacca tcgaaggagc tgtattttga ctacagcttc
    24661 tttttttaga cgatagggaa ttaattttgt ttacactagg atcattcttt ttctttattg
    24721 tattttgagc tatactaaag cataatagga gggtatgatg aaaacgacat ttataagaaa
    24781 aaccggtttt tacggtatgg gtagctcgat tcagctaaga atcaacaatg aaaaacgcag
    24841 tgttctcagt catgaacaaa agataacgct tgatctggaa gtgcccttta cgatgcaggt
    24901 caccttttat tggctaaaaa gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt
    24961 gatcacaatg aattacttga ttcttcaaat ctatccgttt ttatttctag caactgggtt
    25021 gggggccgtt ttcctacaaa atttattttt cagttttttt gcagttattg tgttgattgc
    25081 gttctttttc tatataaaaa accgggcata tttgatcaag gaggaaaatt atggcaaact
    25141 ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa
    25201 atgcacgaaa tatgggattt accgaacttg tcagacgcct agctcaaaga aaacaattaa
    25261 tgatcagaaa atacgaagat gatcttttac agcttgctca gttaaggaat gcgatcgtcc
    25321 atgatcggat cgcagtcgat tttgtcattg cagagccaaa tgattggaca gtcaagcgaa
    25381 tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac
    25441 gtgtaacagg ttttgaacaa gatcttccgt tacttgaact gttaaagata gtagcagaaa
    25501 aaaggtattc acaatttccg ttatacaata aaggaaaatt tgtagcattg attactttac
    25561 gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata
    25621 aacgagcgtt ggatttgatc atccaaaacg gaaagtacac aaattatcat tttgttccag
    25681 ctaaaacaca tatttacgag gttgaagcga tgtttcgtga gcaagggact ttggaagccg
    25741 tacttatcac taaagatggg aatcccgatg gtaatctgct cgggatcgta cgacctcgtg
    25801 atatttataa acaaatagaa aaggattgat tttttgttaa ttatttattg gatattcagc
    25861 gctttgctag taggagtgga tcagttggtc aaatggtgga tcactgataa tctagcgctt
    25921 ggagagacga aaaatcttat accaggtatt ttgtcactga atcatattcg aaacactgga
    25981 gccgcatgga gtatgctaga agggaagatg tggttcttta ctatagtgac actcattgca
    26041 gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg
    26101 attggattga gtttgattct agctggtgcg attggcaatt ttatcgaccg tattcgtctt
    26161 ggctatgtag tagatatgtt ccagacagat tttatgagct ttccgatttt taacgtagca
    26221 gatgtgacac ttgtatgtgg agtgatttgt atgttaattt atattatttt agatgaaaag
    26281 gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca ccatgaatct ggtaggatcg
    26341 ataaagtatt gactaattta ttcaccgatt acagtcgttc gcaaattcag ctgtggctga
    26401 aaaatggagc ggtttctgtc aatggacagc cagtcaaagc aaattacaaa gtaaaacaag
    26461 gagacgagat tactgtcttg atccccgaac cagaaacatt agatgtggtc ccagaaaata
    26521 tcccgttgac gatcgtttac gaagatgaag atgtagcggt gataaacaaa cctcaaggaa
    26581 tggtcgttca tccttcagca ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc
    26641 atatcaagga tctttctacg atcaacggcg ttattcgtcc aggtattgtt caccgaatcg
    26701 acaaagatac ttccggactg ctaatggttg ccaaaaatga ccaggcacac gaatcattag
    26761 ctcagcaatt aaaagataag acctctttgc gcaaatatgt tgctttagtc catggagaga
    26821 ttcctcacga aaaaggaacc atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa
    26881 tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt
    26941 tcgaaggatt tactctggtt gaattaaaac tagaaaccgg aagaacacac cagatccgtg
    27001 tgcatatgaa gtatatcggt tatccgttag ctggagatcc agtatacgga ccgaagaaaa
    27061 ctttgaaagg caatggtcag tttctccatg cgcaaatgtt aggttttacg cacccgacta
    27121 caaacgaaaa gatgatcttt gaagcacctc ttccagaagt ttttgaaaaa actttagaaa
    27181 aattaagaaa aaacattgca ttttaattta gataacggta tagtaattaa caacgaaagc
    27241 ataaaactta atagaattcc tttaagttta gtcccgtgag gctaagaagg gtgggagcaa
    27301 ggatacgtat ggtgcgccat aagtagattg ctcattgaca tagtatagct atcagtgtat
    27361 cagtatacgc tcagtctaaa cattcctcct gtcctcttag ggcaggagtt tttttatgca
    27421 aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt agatcaggta accatgaagc
    27481 gagcattgac tcgaattact tatgagatta tcgagagaaa ccatagtatc caagatatcg
    27541 tattggtagg tatcaaaaca cgaggaatct acattgcatc aagaattgcc gaacgcctga
    27601 agcagctaga agatatcgat attcctgtag gagaactgga tatcacttta taccgagatg
    27661 acaaaaaaga aaatccagaa gaaccggaac ttcattcttc tgacatccca gtttccttag
    27721 aaggaaaaga agttattttg attgatgatg ttttgtatac aggacgaacg attcgagcag
    27781 caatggacgc agtaatggat tttggcagac caagaaaaat ttcattagca gtcctggtcg
    27841 atcgtgggca tcgagaattg ccaatccgtg cagattatgt tgggaagaac attccaacat
    27901 ctcgggcgga agagatttta gtagagatgc aagagcttga tggacaagat cgcatcatga
    27961 ttttgaaaga ggaagattag aggagagaga aacatgtcgg agaaaaaagg atttcataat
    28021 gatgacgtgg tgttagatat ccacgatcgc ccaaccccag gacattgggt aggattaagt
    28081 ttacaacact tgttcacgat gttcggagca accgtgttag tccccatctt agtaggtatc
    28141 gatccaggta ttgcgctagt cagttcagga ctaggtacat tggtgtactt atttactact
    28201 aaaggaaaga tacctgcgta cttaggaagc agcttcgctt tcattgcagc gatgcagctt
    28261 ttgatgaaaa cagatggtta tccagcgatt gcacaaggag cagtcacaac tggattagtc
    28321 tatttgattg ttgcgctgat catcaaaaag atcggttcag catggctgga taaaatcttg
    28381 cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt tgggacttgc ggcaaatgca
    28441 gcaacaaatg ctatgtataa tcagggcacg tatgatttca agtatattgc tgtagcgctg
    28501 atcacattag gattaacaat tttcttcaac atgtggctaa gaggttttct cggattgatt
    28561 ccgattttac taggtattat tagcggatac atcgtagcct tgctgtttgg aatcgtggat
    28621 actaagccaa tcgtagacgc tgcctggttt gctgttccta actttgagat tccctttgta
    28681 caatatgaac caaagctata tcttggagca atcacaacaa tggcacccat cgcttttgtg
    28741 acgatgacag agcatatcgg ccatcttatg gtattgaata aactaactaa acgtaatttc
    28801 tttgaagaac cgggtcttca taagacgttg acaggtgatg gattagcaca aatcgtggca
    28861 gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa acattggtgt tctggctatc
    28921 acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag tatttgcagt cattttagga
    28981 ttcgtaggaa aattaagcgc tttgatttta agtatccctg gaccagtcat ctcaggaatc
    29041 agttttatct tatttggagt aattgccgca agtggaatga agattttaat cgagaacaaa
    29101 atcgattttg ataaaaagaa aaatctgttg attgcttcag ttatcttagt agtaggaatt
    29161 ggcggactcg tctttgaagt aggcacattt acattgtcgg caatggcatt agcaactgta
    29221 cttggcatca ttttaaatct tatcttacca gaaacttcac gaagcgaaga aaactaagga
    29281 ggaaacatta tgatcatcaa atcagaacgt atcagcttaa agcatttatt gaccgttgag
    29341 gcacttactg atcaagaagt tatgggcttg atccgcagag ggcaggaatt caagcaaggt
    29401 gctaattgga ctccgcaaaa gcgccagtac tttgctacaa acctcttttt cgaaaatagt
    29461 actcgcacac ataaaagctt cgatgtagcg gagaaaaaac tagggctaga agtgattgaa
    29521 tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac tatacgatac agtactgacg
    29581 atgtcagcat taggcgtaga cgtagcggtt atccgtcatg gtgatgagaa ctattatgac
    29641 gaactgatac aaagtaaaac aatccagtgt tcgattatca atggaggaga cggaagcgga
    29701 cagcatccaa cccaatgctt gcttgatttg atgaccattt atgaagaatt tggtcatttt
    29761 gaaggtctga aagttgccat cgtaggtgat atcactcact ctcgcgtagc taaatccaat
    29821 atgcagatgt taaaacgtct aggcgcacaa gtattcttct caggtcctag agaatggtac
    29881 gacgatgaat tcgaagtgta cggacattat atgccattgg atgaactact agatcaagtt
    29941 gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg atggaaaaga aagtttctca
    30001 aaagaaggct accatcagga acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa
    30061 catgcaatca ttatgcaccc agcaccagtt aaccgtgatg tagaattagc tgattcatta
    30121 gtggaaggat tacagtcgcg gatcgttcag caaatgagca atggagtatt cgtcagaatg
    30181 gcaatcttag aagcagtgct aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa
    30241 acactcatta aaaacggtca gatcaataca cgaaaaaata tgacgacacc agcagaaatt
    30301 tggatagaag atgggcgaat caaagcaatc ggtacaggat tctcagaagc cgaatttgat
    30361 gaagtgtttg atgcaaaggg acaattgatc acacctggtt tagtggacgt ccatgttcat
    30421 ttgagagagc caggatttac atacaaggag acgatcgaag ctgggactcg ctcagctgct
    30481 cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa atcctgtacc tgatacggca
    30541 gaaaagctga gacaagtata tgacatcatt agaaaagatg cagttgtgaa ggtattgcag
    30601 tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag ttgatcaaga agcactgatt
    30661 gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg
    30721 tatctggcta tgaaagaagc tgcaaaaaac aataaagccc tagtagccca tacagaagat
    30781 gaatctttat tatttggcgg cgttatgcac gctggcaaga aggcagaaga acttggtctt
    30841 cccgggatct taagcgtaac cgagtcttct caaattgcta gagatctgtt gctagctgaa
    30901 gcaactggtg ttcattacca tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc
    30961 agagacgcaa aaaaagcagg aatccacgta acagcagaag tttcacccca tcatcttatt
    31021 ttgatcgacg aagatattcc cgaagatttc ggcttttgga aaatgaaccc gccgctaaga
    31081 ggacgggagg acagagaagc tttgatcgaa ggcttgcttg acggtacgat cgattgcatc
    31141 gctacagacc atgcccccca tggattagaa gaaaaaagcc aaagtttcat gaaatcacca
    31201 tttggtatcg taggaagcga aacagcgttt caactgatct atactcattt tgtagaaact
    31261 ggtcgattca cgttggaaca agtgatcaat tggttggcag ttaaaccggc tgagattttt
    31321 ggtttgaatg caggaacgct aacggttggt gcccccgctg atgtagcagt ttttgatatt
    31381 acacaaactt gcacgatcga taaagaagac tttttatcaa aaggggagaa tacacctttt
    31441 atcggctgga aagtaaaagg cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg
    31501 cagaagggag aataaaagta tggaacgttt gcttatatta gaagatggaa cagttttcga
    31561 aggaaaggct tttggtgcag atgctagtgt cttcggagaa atcgttttta caacaagtat
    31621 gactggttat caagaaacta tcactgatca aagttttaac ggtcaaatca ttacatttac
    31681 ctatcctatg gtgggtaatt atggggtaaa tagagatgat tatgaatcga tttctccaac
    31741 atgcaaagga gtagtagtaa aagaacatgc acgtgtagct tcaaactggc gaaaccaaat
    31801 gacgctagat tcatttttga aacaaaaagg aattcctgga atatcaggta tcgatacgcg
    31861 cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg
    31921 agattccttc gaacatgcat ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt
    31981 gcagcaagtc tcaactaaca aaccttatcc aagtccagga acaggtcgga aagtcattgt
    32041 cattgacttc ggcttgaaac atagtatttt acgagaattg agtaaaagaa actgtaacct
    32101 cacagtactt ccctataaca cagatgccga gacgatttta gcgctatcac cagacggagt
    32161 catgctgacg aatggacctg gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa
    32221 aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc ggtcaccagt tatttgcttt
    32281 agccaacggt gcagatacct ataaaatgaa attcggacat cgaggattaa atcacccggt
    32341 acgagaagta gcgaccggaa gaattgattt tacttctcaa aatcatggct atgcagtcga
    32401 tgaagccacg attgatccag aaaaactcat cgtaactcat gtagaagtaa atgacggaac
    32461 agtagaagga gtcaaacaca gagattatcc cgctttcagt gtgcagtttc atccagatgc
    32521 tgcccctggt ccgcatgatg cactacattt atttgatgaa tttatggaaa tgatggacgc
    32581 aggaaaggag cagtaaacag atgccgaaac gtacagatat caagaaaatt atggtgattg
    32641 gatctggtcc gatcatcatt ggtcaagccg cagaatttga ctacgcagga acacaagcct
    32701 gccttgcctt aaaagaagaa ggatatgaag tggttttagt aaattctaat cctgcgacga
    32761 tcatgacgga taaagaagtg gcagatcaag tatatatcga accgatcaca cttgaatttg
    32821 tatcaaggat tcttcgaaaa gagcgtccag atgctctcct tccaacttta ggcggacaga
    32881 caggcctcaa tatggcaatg gagctggcag catcaggtat tttagaagaa ttgaacatag
    32941 aacttctagg aacaaagctt tctgcgatcg atcaagcaga agatcgtgat ctattcaaac
    33001 agttgatgaa agaattgaat cagccaatcc ccgagtctga aatcgtcaat acagtagaag
    33061 aagctgtaga ttttgcaaat aaaatcggct atcctgtcat cgttcgtcca gcctttactt
    33121 taggcggaac tggtggaggt atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa
    33181 atggattaag tctttccccc gctacccaat gtttgattga acgaagtatt gcaggtttta
    33241 aagaaattga atatgaagtg atgagagatt cggctgataa tgcaatcgtt gtctgcaata
    33301 tggaaaattt tgacccggtg ggtatccata caggtgattc tatcgtattt gcccctagtc
    33361 aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc ttctctttcg attattcgag
    33421 cacttaagat cgagggaggc tgtaatgtcc aactcgctct tgatccacat agttttaatt
    33481 attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga
    33541 ctggctatcc gatcgctaag ctagctgcta aaatagcgct tggattaaca ttagacgaaa
    33601 tgaaaaatcc tgttacagga acgacttttg cagaatttga acctgcatta gattatgttg
    33661 tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa cggagaacgg cttttaggca
    33721 cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat
    33781 tgaaagctgt gcgttcttta gaaatcggta cacaccatat cgaacttcct gagttggcag
    33841 acatcacgga cgatgaccta atccaaaaag tggttcgagc acaagatgat cgcctgtttt
    33901 atgtagctga agctattaga agagggtaca ccatagaaga attagcggat ttgacaaaaa
    33961 tcgatttatt ctttttagat aaactgttgc atatcgtaga aatcgaacag cagttgaaac
    34021 agcagccgtt ggatgacaag atattgaaaa cagcgaaaca aaacggtttt acagacaaaa
    34081 aagtggcgga gctgtgggaa ataaacgaag aagccgttcg ccagatgaga aaagaaaatg
    34141 atttgattcc tgtatataag atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac
    34201 cttattttta tagttcctat gaatatgaaa atgaaagttc acgatcagaa aagccctcaa
    34261 ttctagtact gggatctggt cctatccgta tcggacaagg ggttgaattt gactatgcga
    34321 ctgtgcattc tgtcaaagca atccagcagg caggatacga agcaatcatc atgaacagca
    34381 atcctgaaac agtttcgact gacttttcta tttcggataa attatatttt gagccattga
    34441 ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc gataggagta atcgttcaat
    34501 ttggtggtca gacagcaatc aacttagccg aacctttgac tgctgctggt gtaaaaatct
    34561 tagggacaac gatcgaagac ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc
    34621 tgaaagagtt ggatattcct caacctttag gggatactgc tacaagtaaa gaagaagccg
    34681 tggttatcgc ttcgagaatt ggttatccag ttcttgttcg tccaagctat gtattaggcg
    34741 gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga agattacatg gaacatgctg
    34801 tgaaagcatc tccagaacat ccagtactag tggaccgtta cttgcttggt ggtgaatgcg
    34861 aagtggatgc gatttgtgac ggtgaaacag tactcatccc agggatcatg gagcatattg
    34921 agcgtgctgg tgtccattca ggggattcaa tggctgtgta tccacctcaa acattatctg
    34981 aatcgatcaa gaaaacgatc gaagattaca cgatccgctt agcattaggt ttgaattgtg
    35041 tcgggatgat gaatatccag tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca
    35101 atcctcgagc aagtcggact gttccattct taagtaaggt gacaggaatt ccaatggcac
    35161 aaattgcgac gaaagctatt ttgggagaga aactaaagac acttggttat gaaaacggac
    35221 tttatccaga aacaaaagaa gttcatgtta aagcacctgt cttttctttt acgaaattgc
    35281 agaaagttga tacttactta gggcctgaaa tgaaatcgac tggagaagta atgggatctg
    35341 acagacaatt agacaaagct ttgtacaagg cattcgaagc ttctggatta cacttgcctg
    35401 aatatggagc cgtattattt acgattgcgg acgaaacaaa agaagaagca ctagaattag
    35461 ctaaacgttt tacagaaatt ggctatagtc tgataggaac gaaacataca gctgcttatt
    35521 tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa
    35581 aaaatgtagt cgacttaata agagatggta aaacacaagt ggttgtcaat acgattgata
    35641 aagatcgggg aaatgcttct aaagacggtt tcatcatccg aagagaagca gttgaacatg
    35701 gtattccatt gtttacttct ttagacacag cagatgcgat cgttcgagtg atggaatccc
    35761 gtgctttttc aactaaccct atttaacggc gagcacatgt taagacaaaa agacgaaaaa
    35821 ccgttgcagg aggggacaga tgaaacaaga aatgatggaa atcgttcagc aaaaacagct
    35881 ggcaccaaag atttttgaaa tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc
    35941 aggacagttc ttgcatattc tcgtgccgcg tgcagacttg ctattacgcc gaccgattag
    36001 cttgaatcat atcgaccacg aaacaaatac ttgccgtatc atttatcggg tggaaggtga
    36061 ggggactaga gtcttctcta ctctttctgc cggagaccat ttagatgtca tgggtccttt
    36121 aggcaatggt tttgatttgt ccaatcttca tacaggagac gaagtattta taatcggtgg
    36181 aggaatcggt gtccctcctc tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa
    36241 gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa
    36301 tcgtctagga gaaacaagaa tcgctactga cgatggaaca tacggtatgc acggaaatgt
    36361 cggtaattta ttgttaggaa cgaagacgga acctacagct gtttttgcat gtggttcaaa
    36421 cgggttattg aagacagtgg aacagctgtt ttcttcgcat caaaatgtcc agttatcatt
    36481 agaatcccgc atggcatgtg ggataggggc ttgctatgct tgcgtttgtc atacgccgga
    36541 agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg
    36601 agaggtaatt atatgaatga gaatccttta gcggtcaaac ttcctggcct agaattaaaa
    36661 aatcctatca tgccggcaag cggctgtttc ggttttggca aagaatacgg aaaatactat
    36721 gatttgaatc agcttggatc gatcatggtc aaggcaacga ctccaaatgc ccgctttggc
    36781 aatcccacgc caagagtggc ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa
    36841 aatcctggtc tagacgttgt tatgcataaa cttcttccag aattagagga gtacaatgag
    36901 ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag actatgtaga agtttgtagc
    36961 aaaattggcg aagcaccaaa tgtgaaagca atcgagctga acatttcttg cccaaatgtc
    37021 aaacatggcg gaattgcttt tggaacagat tctgatgttg cttttcaatt aacacaagca
    37081 gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt ccccaaatgt aacagacatc
    37141 gttccaatcg ctcaggcaat cgaagctgga ggggcagatg ggtttacgat gatcaatact
    37201 cttcttggta tgcggattga ccttaagaca agaaaaccga ttttagccaa tcaaacaggt
    37261 ggactctccg gaccagcaat caagcctgtc gctattcgtt tgattcacca agtagcaagt
    37321 atatccagcc ttccaatcat tgggatgggc ggcgtgcaga cagtggatga tgttcttgag
    37381 atgtttatgg ctggagcaag tgctgtagct atagggaccg cgaatttcac cgatccgtat
    37441 atctgtccta aactaattaa agagctccct gtgcgaatga gcgaactagg catcgaatcg
    37501 ctggaaagat taatcaaaga agtgagagag gaacgtgaac gatgaatcaa ctagaccaac
    37561 gaccaattat tgcattagat ttttcaacta ggcaagaggt agaagacttt cttcgatttt
    37621 tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga attgttctac caagaaggac
    37681 ctgaaatcgt acgttatcta aaagaggctg gtcataatgt atttttagat ctgaaacttc
    37741 acgatattcc gaatacggtc gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc
    37801 tcacctgtgt tcatgccgct ggcggtattc gtatgatgga ggcggcaatg cgaggattag
    37861 aagaggggac tccagaaggt ggaaaacgtc cgctacttct agctatcact cagcttactt
    37921 ctacaagtga agaagaaatg catgccgatc aactaatcga agtttcttta gaaaaaagtg
    37981 tgatccatta tgctagctgt gccaaaaaag caggattaga tggtgtggtt tcttctgctt
    38041 gggaagtaga agcgatcaaa gaaatggcag gagacgaatt tgtttgctta actccgggta
    38101 taagaccaga aggaacagtc gcaggagatc agacaagagt agtcacacct tctcaggcaa
    38161 aaaagatcgg ctcaactttt atcgttatag gtcgaccaat cactcagtca actaatcctt
    38221 ataaagcata tcaaacgatt caaacagaat ggagctaacc taaaatgaat gttgaacaaa
    38281 gtatcgcaaa agacttatta gaaattgaag cagtattttt aaatccaagt aatcctttta
    38341 catgggcaag tggaatcaaa agcccaattt actgtgataa ccgaatcacg atgagctatc
    38401 caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg
    38461 aagtacaagt gattgctgga actgcaacag caggaatccc tcatgccgca tgggtagcgg
    38521 aaatactgga tttgccaatg gtctatattc gaagtaaggc gaaagatcat gggaaaggta
    38581 accaaatcga aggacgtata tttgaaggtc agaagatggt tgtgatcgag gatttgattt
    38641 ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa aagagaaggt gcggatatcc
    38701 ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag
    38761 ccgaaatccc tcttatgacg ttgaccaatt attctgtatt gattgaagca gctttagaag
    38821 atcgttatat cgatgagcaa gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa
    38881 attggcagat tgaagcgcgc ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc
    38941 atcaattgga atagcaaaaa tccaaaaact gattgcgaga agcatctttt catcaggtat
    39001 gaaaagaaac ttctatcgtg atcagttttt gattttttta aaattcattt ttctaagact
    39061 ggttagcaga aagaagagaa taatctcttc tcttttttct attatatata gattttctct
    39121 attttttggt aagggagctg tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag
    39181 ttatttaatg atatgatatt cggaaaaaga aatctcaaag ggaggtaact tttatggggt
    39241 tgaatgaatt tattttagga ttgaacaatt tagagacgat cacaagggca cctggatttt
    39301 ttaaatatac agagcataca gttgcagcac attcgtatcg tgtagcatct attgcgcaag
    39361 ttctaggaga cattgaagaa gcgaatggac aaccgatcga ttggaaactg ctctatgaaa
    39421 aatcattgaa tcatgactac acagagcgtt ttattggtga tatcaaaacg ccggtgaaat
    39481 acgcaagcaa agagttaaga gggatgctgc aaacagttga agaaaagatg acggacgaat
    39541 tcatcaacca agagattcca gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag
    39601 gaaaagatga aaccgtagaa ggagaactat tagccatttc agataaggtt gaccttcttt
    39661 atgaatcttt tgatgaaatc atcaaaaata acccggaaaa agtttataag gagatgttca
    39721 ttgaggcagt gactacgatt aaaagctttg aacatcggcc ttctgttcag tatttcttta
    39781 aagagatatt tccggaacta ttgaaccagg aattttatgg aaaagaggcg tttttagaaa
    39841 ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg atatgagatg cagggggaag
    39901 tatagttgaa aaagaatatg gatgtcgaat ggggctattc tggagacaaa ggtccggaaa
    39961 attgggcgag tttatgtgat tggtttgcga gaggtgcgga atttccattg cagtcgccta
    40021 ttcatttgac caagggggaa gaaaccaaaa tagaaggaga cacgctttcc tttcattatc
    40081 aaaagcaacg gtttactgac aaggaattca aaaacacgat tcatttagta ccttttgacc
    40141 aactcagtta tgtagagttc aagaaagaac gctattattt gacagatatt catttccact
    40201 tgccttctga acatatcatc aacggaaaac aggaagatat tgaatttcat tttgttcata
    40261 tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag
    40321 gttggctgta taatcaagaa aatggggaaa gttggaactt agaaaaacat gagcattggg
    40381 taaatccgga tgtgttcttt ccggagcaaa aaagccattt ccattatatt ggctcattga
    40441 caactccgcc taccagcggg ccaatccaat ggtttgtaat ggatcatgta ggaaaactga
    40501 accaagagtt tcttctcccc ttcgatggtc agtatgctcg accgaacaac cgaccaatcc
    40561 agcccttgaa tggaagagaa atttattatt tcgaggagtt tgagcaatga atattcagca
    40621 gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc agagaagcgg caaagaaaat
    40681 gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa ttagaaaccg agttagggat
    40741 cacgctgttt actcgaacga atcgaggggc atatttaaca gaagaagggc aagacttctt
    40801 aaatcgtgcc gagaaaatat tggtccagct tgatacatta gaaactcact atctaacgag
    40861 tgaaaagaga gacaattttt cgatcgctgc ccagcattat gatttcttag gtcctttgac
    40921 cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa tttcggatca tggagacgac
    40981 aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt gagatcggca ttatttatat
    41041 gaatgagcat aatcaagctg gtatcagaag atatttacaa caaggagagc taagtggccg
    41101 cagcattgga aaattcaaaa cgcatatttt tttaaggcaa ggacatccat tagctgaaca
    41161 cgcagagatc accaaagaag aattgttatc ttatcctcaa gttcgtttta cacaggatgg
    41221 aaataatttc ccgtattttt atgaagacct tatcgagatc cctgatcaag aaacagtgat
    41281 ttatacgagt gatcgaggta ctttgatgaa tatcgttctt gaaactgatg cttacgcttc
    41341 aggatctggt attgtgattg gagggattcg cgagcacttg cggttaatcc ctttggcaga
    41401 aggcgaacta aatgaatttt atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc
    41461 tcaaagattt atcaaagagc ttactcggtt actggaccag cagagctaag aaaagcagat
    41521 gagaacgctg tttattgaac accgttgatc aaaaaagagg ggttgtgaca gaattttgtc
    41581 acaactcctc tttttgtagc aaacaattta taactaattg ctgttgtggg aaagtacgca
    41641 aatatgattc tagaaacact cattgattac ctgttttgtt tcatagctgt aattttttct
    41701 tcttctggtg tcacgataat cgtcttttgg ttttcataaa taacataacc aggcttggcg
    41761 ccatttggtt ttcggatatg ctttacctgt acaagatcaa ccggcacttg ggcagagtat
    41821 cggtattttg aaaaataggc agctaactcg gcagcttctg taattgtttc atctgagggc
    41881 ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg
    41941 gtttttttag ctgtcttcat tgtcagctgg tcattttgaa gattgttttt accaacaaga
    42001 atgagcgttc cgtcagaaga aaagtattga tcaggctgag atggtttctt acgtttttgt
    42061 ttttttaagt tcttcttttt caggtatcct tctgaagtca attcttcttt aattgcttcg
    42121 atatccattg ggccggcaat ttccagttga gataagacag attcaagata ttggatttca
    42181 tctttggctt cttggatttg ttcgccaatc agcttaacag cgttcttcag cttttggtat
    42241 cgatggaaat atttctgtgc attttgattt ggtgtcaatg ccggatctag tgctattttg
    42301 attggccgat cttcttcata ataattaggt aaaacaactt cttttgcgcc gcgtggtact
    42361 tgtgtcagaa aagtagtaag tagttcgcca tttctacggt aattctcagc gttttcggat
    42421 tctttgagtg tttgttcacg ttttttcaat ttatttttat ttcgttttag ttcattttcg
    42481 atttttcgaa tcagttctcc gccttgttgt tttgcccgat ctttctctgc tttttcgtga
    42541 taatagctat ctaataattg actgagtgtt ggataagctg ttacagcaga tgcctgttcg
    42601 gacaatacct gataagcaat cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta
    42661 ggaataggct gatggctgat ggctgagaaa aattcgtccc atacaaccat cttttcatta
    42721 ggacgttcca gtaatctttt ggtcagctct tgagcgatat cgaaaccgat tccctgaaac
    42781 tgacgttgga ttgcttttgg atctagttca gtttgggaaa gccgctgaaa gattttttct
    42841 ttctcggagc taaatgggtt caagacctct tgttttggcg gagcaacata ttctacacca
    42901 ggaagcaaag aacgataagt gttttgagag cttccaatgt gtttgatcgc atccagtatt
    42961 tttccagttt ctcgattaac taagacgatc gtactatgac gtcccattaa ttcaacgatc
    43021 aatatgatat tttgtagatc acccaactcg tttcgtttag caaagtggaa atggatcact
    43081 cgatcatttt cgatttgttc gatgtcttct aaaatagcgc catccaaata ttttcgaagc
    43141 atcatcacga agttaggcgg attatcagga ttctgataat cgatttgggt aatctgtaca
    43201 cgagcataac ttgggtgcgc agataataat aatcgatggt ttttgccgcg tgaacgaatc
    43261 accagcacga tttcattttc ataaggctga tggattttcg agatccggcc agatagtaac
    43321 gtttcacgca attcgttgac catcgcgtgg gtaaatacac catcaaagga catggttcat
    43381 tcctcatttc ttttcataag tatattcatt ataacgaaaa aaagaagaaa aaccaatttg
    43441 gccgaatagt aaagcaaaag tctagaaaaa tcttggacta attacataaa aaacagtgtt
    43501 tctatcgttg aaatccaaat ttttaggta
//
LOCUS       DADOZM010000008        42940 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.008, whole genome shotgun sequence.
ACCESSION   DADOZM010000008 DADOZM010000000
VERSION     DADOZM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.008"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(122..1096)
                     /locus_tag="J8I47_000372"
     CDS             complement(122..1096)
                     /locus_tag="J8I47_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAZ6321838.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1077..2960)
                     /locus_tag="J8I47_000373"
     CDS             complement(1077..2960)
                     /locus_tag="J8I47_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAZ6321839.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(2978..5203)
                     /locus_tag="J8I47_000374"
     CDS             complement(2978..5203)
                     /locus_tag="J8I47_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAZ6321840.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5318..6352
                     /locus_tag="J8I47_000375"
     CDS             5318..6352
                     /locus_tag="J8I47_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6321841.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6419..7432)
                     /locus_tag="J8I47_000376"
     CDS             complement(6419..7432)
                     /locus_tag="J8I47_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6321842.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7510..7698)
                     /locus_tag="J8I47_000377"
     CDS             complement(7510..7698)
                     /locus_tag="J8I47_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAZ6321843.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8138..8266)
                     /locus_tag="J8I47_000378"
     CDS             complement(8138..8266)
                     /locus_tag="J8I47_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAZ6321844.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8269..9642)
                     /gene="rlmD"
                     /locus_tag="J8I47_000379"
     CDS             complement(8269..9642)
                     /gene="rlmD"
                     /locus_tag="J8I47_000379"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAZ6321845.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9753..10808)
                     /locus_tag="J8I47_000380"
     CDS             complement(9753..10808)
                     /locus_tag="J8I47_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAZ6321846.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10818..12248)
                     /gene="gatB"
                     /locus_tag="J8I47_000381"
     CDS             complement(10818..12248)
                     /gene="gatB"
                     /locus_tag="J8I47_000381"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAZ6321847.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12245..13717)
                     /gene="gatA"
                     /locus_tag="J8I47_000382"
     CDS             complement(12245..13717)
                     /gene="gatA"
                     /locus_tag="J8I47_000382"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAZ6321848.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13717..14022)
                     /gene="gatC"
                     /locus_tag="J8I47_000383"
     CDS             complement(13717..14022)
                     /gene="gatC"
                     /locus_tag="J8I47_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAZ6321849.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14128..16164)
                     /gene="ligA"
                     /locus_tag="J8I47_000384"
     CDS             complement(14128..16164)
                     /gene="ligA"
                     /locus_tag="J8I47_000384"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAZ6321850.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16181..18415)
                     /gene="pcrA"
                     /locus_tag="J8I47_000385"
     CDS             complement(16181..18415)
                     /gene="pcrA"
                     /locus_tag="J8I47_000385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAZ6321851.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18570..20129
                     /locus_tag="J8I47_000386"
     CDS             18570..20129
                     /locus_tag="J8I47_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAZ6321852.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20240..22171)
                     /locus_tag="J8I47_000387"
     CDS             complement(20240..22171)
                     /locus_tag="J8I47_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ6321853.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22189..23103)
                     /gene="pfkB"
                     /locus_tag="J8I47_000388"
     CDS             complement(22189..23103)
                     /gene="pfkB"
                     /locus_tag="J8I47_000388"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAZ6321854.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23100..23852)
                     /locus_tag="J8I47_000389"
     CDS             complement(23100..23852)
                     /locus_tag="J8I47_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6321855.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24011..24220)
                     /locus_tag="J8I47_000390"
     CDS             complement(24011..24220)
                     /locus_tag="J8I47_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321856.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24231..25559)
                     /locus_tag="J8I47_000391"
     CDS             complement(24231..25559)
                     /locus_tag="J8I47_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321857.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(25924..26511)
                     /locus_tag="J8I47_000392"
     CDS             complement(25924..26511)
                     /locus_tag="J8I47_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAZ6321858.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26594..27844)
                     /gene="clpX"
                     /locus_tag="J8I47_000393"
     CDS             complement(26594..27844)
                     /gene="clpX"
                     /locus_tag="J8I47_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAZ6321859.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28091..29380)
                     /locus_tag="J8I47_000394"
     CDS             complement(28091..29380)
                     /locus_tag="J8I47_000394"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAZ6321860.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29534..30466)
                     /locus_tag="J8I47_000395"
     CDS             complement(29534..30466)
                     /locus_tag="J8I47_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321861.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30623..31282)
                     /locus_tag="J8I47_000396"
     CDS             complement(30623..31282)
                     /locus_tag="J8I47_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAZ6321862.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31285..32310)
                     /gene="sppA"
                     /locus_tag="J8I47_000397"
     CDS             complement(31285..32310)
                     /gene="sppA"
                     /locus_tag="J8I47_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAZ6321863.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32323..32613)
                     /locus_tag="J8I47_000398"
     CDS             complement(32323..32613)
                     /locus_tag="J8I47_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321864.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33022..34827
                     /gene="glmS"
                     /locus_tag="J8I47_000399"
     CDS             33022..34827
                     /gene="glmS"
                     /locus_tag="J8I47_000399"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAZ6321865.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(34899..36254)
                     /locus_tag="J8I47_000400"
     CDS             complement(34899..36254)
                     /locus_tag="J8I47_000400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAZ6321866.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(36278..37447)
                     /locus_tag="J8I47_000401"
     CDS             complement(36278..37447)
                     /locus_tag="J8I47_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321867.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(37444..38331)
                     /locus_tag="J8I47_000402"
     CDS             complement(37444..38331)
                     /locus_tag="J8I47_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAZ6321868.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(38520..42212)
                     /gene="nifJ"
                     /locus_tag="J8I47_000403"
     CDS             complement(38520..42212)
                     /gene="nifJ"
                     /locus_tag="J8I47_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAZ6321869.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            42411..42932
                     /locus_tag="J8I47_000404"
     CDS             42411..42932
                     /locus_tag="J8I47_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAZ6321870.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        11692 a         9275 c         7751 g        14222 t
ORIGIN      
        1 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
       61 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
      121 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
      181 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
      241 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
      301 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
      361 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
      421 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
      481 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
      541 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
      601 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
      661 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
      721 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
      781 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
      841 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
      901 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
      961 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
     1021 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
     1081 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
     1141 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
     1201 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
     1261 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
     1321 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
     1381 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
     1441 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
     1501 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
     1561 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
     1621 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
     1681 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
     1741 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
     1801 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
     1861 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
     1921 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
     1981 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
     2041 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
     2101 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
     2161 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
     2221 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
     2281 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
     2341 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
     2401 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
     2461 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
     2521 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
     2581 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
     2641 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
     2701 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
     2761 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
     2821 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
     2881 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
     2941 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
     3001 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
     3061 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
     3121 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
     3181 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
     3241 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
     3301 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
     3361 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
     3421 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
     3481 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
     3541 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
     3601 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
     3661 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
     3721 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
     3781 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
     3841 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
     3901 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
     3961 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
     4021 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
     4081 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
     4141 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
     4201 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
     4261 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
     4321 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
     4381 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
     4441 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
     4501 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
     4561 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
     4621 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
     4681 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
     4741 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
     4801 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
     4861 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
     4921 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
     4981 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
     5041 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
     5101 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
     5161 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
     5221 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
     5281 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
     5341 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
     5401 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
     5461 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
     5521 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
     5581 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
     5641 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
     5701 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
     5761 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
     5821 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
     5881 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
     5941 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
     6001 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
     6061 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
     6121 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
     6181 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
     6241 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
     6301 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
     6361 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
     6421 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
     6481 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
     6541 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
     6601 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
     6661 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
     6721 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
     6781 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
     6841 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
     6901 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
     6961 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
     7021 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
     7081 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
     7141 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
     7201 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
     7261 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
     7321 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
     7381 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
     7441 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
     7501 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
     7561 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
     7621 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
     7681 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
     7741 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
     7801 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
     7861 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
     7921 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
     7981 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
     8041 agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta agaaacaata
     8101 acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag attttagggc
     8161 cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat cggcctccac
     8221 cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt attctccttt
     8281 aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta caggctgaac
     8341 tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa aagttgccgg
     8401 attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt caataaaatg
     8461 atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga tccctttttc
     8521 ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat tttcgatttt
     8581 attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt ccatcccata
     8641 caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg aataagcgtc
     8701 gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt acagatactc
     8761 tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg tttttcctaa
     8821 tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc ctaaaatcac
     8881 atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca attcttcatg
     8941 aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa ctaaaacgac
     9001 catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct gtccttcatt
     9061 tttttcttca ttatatcctc gtacttggaa ccgttgcaag atatctctga ctactttgat
     9121 tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta gatcatggct
     9181 attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag ggatctgtgc
     9241 tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag gaacttctgg
     9301 catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga caagctgttg
     9361 ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat tgttttctac
     9421 aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc caaacttatt
     9481 gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt cgataaataa
     9541 tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga cgatttcgac
     9601 tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct cctttaagcc
     9661 tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact aaaaccggca
     9721 ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg tgtttcgtct
     9781 gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc ttttacgaat
     9841 tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc atcaggaatc
     9901 tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg catcggtgct
     9961 tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc atcttctgtt
    10021 tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac atgtcgaccg
    10081 ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt cttcacaatg
    10141 attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc gaatcctcca
    10201 acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc cccttcatca
    10261 tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc tttcgcaaga
    10321 taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt caattctgtc
    10381 aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc gatgtccatt
    10441 tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc tctaggaatt
    10501 tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat tttaggacgg
    10561 tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc accagctgcc
    10621 actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc tgaatctggt
    10681 tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc ctgtaggata
    10741 tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat tacgcgtgct
    10801 tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt attcacgaca
    10861 ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa gccaaccgca
    10921 cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa aatttcatta
    10981 atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt ttcaactact
    11041 tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc gatttttgag
    11101 ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg tgtcaatttt
    11161 gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc catcaaccag
    11221 ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa atcagacatt
    11281 tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc acggatatag
    11341 cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg aatccattcg
    11401 tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc actagaccct
    11461 tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg tgtttcttgt
    11521 tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa agctaaacct
    11581 tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc gaactcttct
    11641 tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc ttccattttc
    11701 acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata agcgtatgct
    11761 tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt gccttgtcgg
    11821 ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc atcttcttct
    11881 aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat ttcgatccat
    11941 ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg attatctggg
    12001 taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt taatgccaaa
    12061 gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac gcctggataa
    12121 ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc tggcgctgga
    12181 gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat gactgtttca
    12241 aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat tctgttgctt
    12301 gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta ccgatgatct
    12361 gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct ggaagtccag
    12421 ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga tcgttaatgt
    12481 tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag tcataatctg
    12541 caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct tttttgaagt
    12601 aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg cgttttactt
    12661 cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat ttcacatttt
    12721 cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt gcttctgatg
    12781 aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagcta acttcttcca
    12841 caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct tttacgccag
    12901 gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct ttgatgtcgc
    12961 ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg tctttttcgt
    13021 cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg gtcattggtc
    13081 cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca cggccataag
    13141 taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt cccccagtat
    13201 cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa ccaccagaag
    13261 agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa taagaagttt
    13321 ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg atcatatctg
    13381 cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg taaaggatct
    13441 tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata ccaattggaa
    13501 taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt gcctgttcca
    13561 atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt tctttgattc
    13621 gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca agcatatgat
    13681 gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct ccccattatc
    13741 gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt tcaataattc
    13801 atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg tttcaaccac
    13861 attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa ccatatcgat
    13921 gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg caagttttgc
    13981 taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc ttctttctaa
    14041 acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga aattgtttat
    14101 tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt tagccaatgc
    14161 atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt tggtcagctt
    14221 gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc taccagtcac
    14281 tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc gtgttagttt
    14341 accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca attgggcatt
    14401 tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca tcaattcatg
    14461 gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac cgatcgtatc
    14521 aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac cgaaatgttc
    14581 tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga aaatcaaccg
    14641 ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg ctgatttttc
    14701 ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat cagcaacatc
    14761 tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc catcgatatt
    14821 catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag ggcatttagg
    14881 attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc cacaaactgg
    14941 acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta acacgacttg
    15001 cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt tcaagcggat
    15061 atccttcaac ttaatataat caccgttatg caggctggca cggcttactg tcgttcctgc
    15121 tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac cgactgtcca
    15181 ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag ctgctgccca
    15241 tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat tcactttgat
    15301 gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt cttcaatgta
    15361 tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt ggaatccaat
    15421 tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac cgaaatcagc
    15481 tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat ctaactgacg
    15541 caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct ctgcttctct
    15601 gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc ttacttcgat
    15661 gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt tttcagtgat
    15721 gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc catcttcata
    15781 acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa ctacacgtcc
    15841 cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga aaacgtcgtt
    15901 caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata cttttccgcc
    15961 tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct cgattgctac
    16021 taattctgcg tattctttat cataaacata gtcttccact gttggtttat cttctacgta
    16081 gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt tagcagcttc
    16141 gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt tcaattggcg
    16201 caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct agttctagat
    16261 ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg tgtttgactt
    16321 tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag ggaacaaccg
    16381 ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc gtttttttag
    16441 gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct acaaaacgag
    16501 aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt gttaaataca
    16561 acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc tccagttcgc
    16621 tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca atcaggaata
    16681 caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact tgggaagcat
    16741 cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag accgttagtt
    16801 tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg tcgaattctt
    16861 gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct aatgtatttt
    16921 ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc aattctgtca
    16981 ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat gcacctaatt
    17041 gctgtcctgc ttttccaccg atatttgcca gatccacatt ttgcgctgct tcaagcagtg
    17101 gccattcatg aagagaagca aaactgcgta gtttttcgat agatcctggt ccgatgcctc
    17161 gtttgggcga attaacgata cgctcaaaac tgattgaatc ttgaggattt accagcacat
    17221 tcaaatacgc taaaatatct ttgatttctt tacgatcgta gaacttgtgc cctccaacca
    17281 ttttataagg aatatttgct ttcaacagag tttcttccat tacacgagat tgtgcgttcg
    17341 tccggtacaa gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca
    17401 tccggtctac gataaaacgg gtttcgtctc gttcattatc tgcacggtag taagtgatct
    17461 tgtttccttc tttgttttct gtccacaagt tttttggttt tcgattgcta ttgttttcaa
    17521 tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg atagttttgt tctagcaaaa
    17581 ttactgcagc atctggataa tctttttcaa aatccaagat gttctgcata tctgcaccgc
    17641 gccagccgta aatactttga tctgcatctc ccacgacgca caggttccta aatctacctg
    17701 ccaataaatt gactagcgta tactgtgcgt gatttgtatc ttggtactca tctacatgaa
    17761 tataatggaa tttgttttga taatacgtga gtgaatcagg atgttcttca aataatcgaa
    17821 tcgtattcat gatcaggtcg tcaaagtcca tacattgatt atattgaagc tctttttggt
    17881 aagcagcata acattttgcc gcaatctctt cgtaataact gccttgcatt tttgcaaaat
    17941 cctgaggggt ctgcaggctg ttttttgctt gagagattgt tcctaatata cttcgaggat
    18001 catatttttt cggatcaatg ttcagttctt ttagtatccg tttcatcaag gtcagctgtt
    18061 cagaagaatc gatgattgta aagttccggt tatacccgat aaaatcaaca tctcttctta
    18121 aaatacgcac acacatcgag tgaaaagtcg atacccagac atcttcgcct ccagatgcga
    18181 gaatcgcatt gacacgttct ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta
    18241 ggatgttcca aggattgact tctttttcct caatcaaata ggcaatgcgg tgtgtcagca
    18301 ctctcgtctt tccacttcct gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa
    18361 cagcttcttt ctgacggggg ttcatcccgt ttaataattc agctttggat gacattaaca
    18421 acatcctttc ttttatcaat cttgttaatt atagcatttt cttggctttc attaaactag
    18481 ctagttcaaa atcatttgtt tctttttatg gatttttcct tgtcaattcg ttgatttcgc
    18541 gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag
    18601 ataagactca gatatatttt attctaaaag gaatatttgt tggactgctg acaggaatcg
    18661 tcgttagcct gtttcgatta tctatcgaaa agctaagcga tttagttcga agtatctatg
    18721 aaatgagtcg ggaacagccg atttacttaa ttggcctagc tatttgctgc atccttgcag
    18781 catttttcgt gggttatctg gtcaaaaatg aaccggatat caagggaagc gggattccac
    18841 aagtagaggg acagcttcgc ggggaattat caatgaactg gttctctgtt ttatggaaaa
    18901 agttcattgg cggtgtactt tcagttggtg cgggcctttt tctaggtaga gaaggacctt
    18961 ctatacagct tggcgcatcc attggacaag gagctggaca attgtttcgg agtccatctt
    19021 cagaagaaaa gattttaatt tctagcggtg caagtagcgg acttgcagca gcattcaatg
    19081 ctcctatcgc aggattactc tttgttttag aagaaatcca tcactccttt tctccacttg
    19141 tttggctgac atcatttgct tcagcaatca ctgctaactt cgtctctctt tactttttcg
    19201 gcttgagacc tgttttatat ctaggtgacc ttccttcttt gccgttgaaa tattatggtt
    19261 ccttagtgat cttaggagta cttctaggca tcttaggttt tatttatcag aatgtcttgc
    19321 tggctttgcc aaaattttac aaccgattgc ctttaccagc ttattttcac gggttcgttc
    19381 ctttcttgct gattttgcca attggttatt tatggcctag tactctaggc ggaggcaatc
    19441 aaatcgtttt agcttttgga catgcttcct tgccaatcat tatgattatc ggtttgtttg
    19501 ttcttcgatt cgttttttcc atgatttctt acggtggggg tcttccaggg gggattttcc
    19561 ttccgatatt gacattgggg gctttaatcg gtagctttta tggaaacatt atggttgatt
    19621 tcgggatgga tccaatctac atcaaggatt ttgtcgttat tgccatggct ggttatttca
    19681 cagcgattgg aaaagcaccg ctgacagcaa ttattttagt gacagaaatg gttggctctc
    19741 ttaatcatct gatgccttta ggattggctg ctcttgttgc ttatatcgtc aatgaccttc
    19801 tcggcggtaa tccaatttac gaatcattat tggaacgtct acttggaaat actcagaccg
    19861 cctcactaaa aggtgcaagg acaacttttg attttccagt aacggcagag agttctttag
    19921 acggattaat ggtacgagac ttcaattggc ctaaaaatat gctgctaatc tctatacgca
    19981 gaggaaatca ggaaatcttg actcacggtg atacgatcat gaatgtagga gatattttgg
    20041 ttatcttgac tgatgaagca aacttgccaa gaatcaagca ggaaatcgag gctaaatctt
    20101 ctatcacaaa tttgaagcaa tcattttaac gaacatggaa aaactatttt acaacaacta
    20161 ttcatctaca ctacttgatt ctatatgatt ctataaaaaa agaagaggct gggaaaaccc
    20221 agcctcttct tttttatttc tatgcttctg ctactgcagg agcttttctc aataaaccat
    20281 aaatcacacc agatacgact gctccgatag caataaacac caaatacatg accgggtgac
    20341 tcagtaagaa tacaacgaaa atcccgccgt gaggagccag taatttgatt ccgaaagctc
    20401 cgactaatcc gccagttaaa gcagaaccaa taataaaact tggaatggca cgaatcgggt
    20461 cagctgctgc aaatggaatc gatccttccg tgacaaacga tagtcccatc acgatgttcg
    20521 tcaaaccagc atcttgatct gttttgctga atttattctt aaacaaacgt gtagcaacga
    20581 aaattgccaa tggaggtacc attcctccag ccataacaga agccatcacg acacttccgc
    20641 cagttgcaac agtcgtagct aaagttgctg taccaaacac ataagcggct ttgttgattg
    20701 gaccgcctaa atctgccgcc atcattccag ctaataatgc accaagtaaa gcagcattcg
    20761 aaccactcat acttgtcaag aaaccgttca atgcatcatt gattgctttc atagggacat
    20821 tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa cactggataa aagaggatcg
    20881 ttttaattcc atccaatgat tttggtaagt tcttgaataa tttacgtaaa aatacgatga
    20941 cataaccagc taagaatcca ccaatcagtg cacctaagaa tcctgcaccg cctgtattag
    21001 ctaatgcacc agcagcaaat ccaacgatca aaccagggcg gtcaccgata cttgaagcaa
    21061 taaatcctgc taataccggc agcatgaaac caaatgcagc ttgtccgatt tggttgaacc
    21121 agctagcagc ttgattgtaa ttccctagat ttgataattg atcatgtggc acgccaataa
    21181 attggtcgat catgaaagaa agcgcaatag cgattcctcc accaataacg aaaggtaaca
    21241 tgtgtgatac gccgttcatc aaatctttgt aaattctttg tccgatagta ccatccgcat
    21301 ttccttcttc tgaactgctt tcttttccat caccatggaa tactggtgca gtaccactaa
    21361 gggctagatt gatcaactct tctggtttac ggataccatc actgacagga cgattgatca
    21421 gttcttttcc gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg attacacctt
    21481 ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt
    21541 cgactttgat ctccacaccc atttctttcg cttttttctt caaagcatct tctgccatgt
    21601 aagtgtgagc gatccctgtt ggacaagctg taacggcaac aacgaatttt ctttctggat
    21661 gagcagaagc ttctgcagct tgtgtttctt gttctgcttt ttcttctgct tctttttgct
    21721 cttcagctgt ttggaacaag tcttggactt cttctggtgt atttgcttct tttaattttc
    21781 ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt
    21841 cgtttgcgcc ttctggagct gcgatcatga aaaacagata agttggctgt ccgtctaagg
    21901 cttcataatc tactcctttt tggctttttg cgaataaaac tgttgcttct ttaactgcac
    21961 tgtttttcgc gtgaggcatg gcgattccat caccaagacc tgtagaagtt tgtgcttcgc
    22021 gggctagaat tccttcttta tatgtttcga tatcggaaat ccggccggca tcaaacattt
    22081 tttggaccat ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg
    22141 cttctttgac cattaaatcc ttgatattca tgattatcct ccttgaaact aatcaatttt
    22201 tgtgatttca acttctggca gcaactcatc aatcaattcg cgagctgcca agtcatcaga
    22261 aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag gcttcgattg gatctttcgt
    22321 acggctgtaa gccccaataa agccagcaat cattgaatcc cctgcaccta ctgaattttt
    22381 caatgggcga actaatacat tcgttttata agttccttgt tccgtgaaga gcaaagcacc
    22441 gtctccagcc attgagatca gcacattctg tgccccttca gctagtaatt ttttcccata
    22501 cggaatgatt tcttctacag attggaaggt tgtgttgtaa agctcagcta attcgtgatt
    22561 gtttggtttg atcaacaacg gtttcttaac aagtgcattt tttaggtcat cacctgttgt
    22621 atcaatcacg aaatctgcac ctttttctcg aatgatatca atcagttctt gataaaatcc
    22681 ttttcgcagg ctagctggcg cgcttcctga caatacgacg atatctcctt gtcctactgt
    22741 tcctaatgct tgcttcaact catcgatttc ttcatctgaa atagatgggc ctaagccatt
    22801 gatctcagtt tcagtgtctg attttagttt gatattgatt cttgtatctt cttttacggt
    22861 tgtgaacaaa gactggattt gttctttttc aagccacttc gaaataaatt cgccagtgaa
    22921 ccctcctaaa aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac
    22981 attgatccct tttcctccag gtaacttgaa gtcttccttc attcggttca aatcgcctag
    23041 ttttaaccca tctacgcgta cgatataatc aattgatgga ttaagtgtta ctgtgtaaat
    23101 cattgtattg cctccttaat tgttgtcttt tcacggaatt cattaacgaa ttctaatgga
    23161 caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg
    23221 ttgaatttgg tatgatcaat caatacaaat gtctcttcgg aatttttcat ggctaaacgt
    23281 ttaacagctg cctcttctgg atccggagtc gtgaatcctt gttcaagatg ggcaccgttc
    23341 attcccataa atgctttatt aaaatgaaaa ttttttagtt gttctatcgc attagatcct
    23401 aaaacagcat tagttgccag ctttatcgtt ccacctaaaa taatggtagc aagctccaaa
    23461 tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc
    23521 agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta aataaatgac atcttcgtca
    23581 ttaatggttt cagctgccga tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat
    23641 ttttcactca tattctgctc aaaattcaat aattgctctt tcttagctcc gccatgaact
    23701 ctttctagaa acccactatt ctccagctct tgtaaatcac gtcttatcgt tgattcagaa
    23761 gcattcaaaa ggtttacaag atcttgtgat ttcacgattt tctgctgttc aagcaagtct
    23821 aggatttttt gtctgcgttc ctctgtaagc attttcacga tcacctcaat acaaataata
    23881 gcatattttt tttctactag caatataaat cagtcaaaaa cgttcataaa aattataaat
    23941 cagtcaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt ttgtctatca
    24001 tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa acagaagcag
    24061 gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac atgaccaaaa
    24121 aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg gataataatg
    24181 atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc tcatattttt
    24241 tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg cagatgctgc
    24301 tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg tttgaaggta
    24361 acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga ttctgaagga
    24421 acatagattc ttaaggataa aagatactct tgatattttt catctgttac ctctacttct
    24481 agatattccc taatataata acaataagct tcgattccat tatccaaagc aacagtcgga
    24541 tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg aaaatacgcc
    24601 tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg gatatcataa
    24661 ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg tgcattctga
    24721 tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg cggtttcaat
    24781 aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct aaaataggat
    24841 tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag ctggatacat
    24901 atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag aaaaatcggt
    24961 ttagggatga tttgatgata atagcgacta taatacattt cccaacacga agtgaaacat
    25021 aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac tacttccccg
    25081 ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa caatagccgt
    25141 tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat ataggcgaac
    25201 accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg cttttctttg
    25261 aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa gcgaaaacta
    25321 cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga tgctagcatt
    25381 attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa agagcggata
    25441 aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc ctgttttttt
    25501 tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc gccaatcatt
    25561 ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa gaaaaactaa
    25621 aactttatgt cttagaaacc aatttggacg attacatgat agactaagta aaaagaaaca
    25681 aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat aaacttttcg
    25741 aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc gatcgatttc
    25801 taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa gttaagctca
    25861 aaaaaagact taccagccaa acttttatga aagagtttga ctggtaagtc ttttttattc
    25921 tttttatgat tcaattgctt gttcgattgc tgcccacgct tcttcttttc cctgttttgt
    25981 aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa tccaacgctt ttttgatttc
    26041 gctctcatgc ttgttccatt taccacgagg gatcttatct gccttagtgg caacaacaat
    26101 tacagggata tcataatact tcaaaaattg atacatctgg atatcttctt gagaaggctt
    26161 atgacgcaaa tctactaaag aaacgaccgc acgtagctgg tctctagtcg ttaaatatgt
    26221 ctcgatcatc tttccccatt ttgctcgctc agttttggat acttttgcat acccatatcc
    26281 cggaacatcg acaaaatgta atgcgttctc gatcaaataa aaattcaatg tctgggtctt
    26341 tcctggctta ccggaagtac gggctaaatt ctttcgatca atcaatgtgt tgataaaaga
    26401 agatttccct acatttgatc gtcctgccag agctatttct ggcaattgtg tatctggata
    26461 ttgttttggt gccactgcac ttatgataat ttcagcttga tggactttca tggtctctct
    26521 ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa agccaaaatg acttttacgg
    26581 cagcctcttt tcatcaaccc gcttttttct ctgaatcttt atagattact gttggctcac
    26641 cagacaattc agctgcttct tttgtgataa tcactttttc gatgttttca tttgaaggga
    26701 tatcaaacat cacatccatc atgatttctt caatgatgga acgaagtcct cgagcaccgg
    26761 tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc ttcaggttcg aattcaagtt
    26821 cggtattatc tagagaaaga agtttttgat attgtttgac tagcgcattt ttcggctcag
    26881 tcaagatccg taccagatca tctgttgtca atttctctaa agctgccata acaggtaatc
    26941 gtccgataaa ctcagggatc aatccgaatt ttagtaaatc ttcaggaatg atatgctgca
    27001 tgacactttc gtcttcagaa agtttttggt tttgtgtacc aaatccaatc gtcttttcgc
    27061 ccatacgatt cttcacaatc gtttcgatgc catcaaatgc accacctacg atgaacaata
    27121 tgttcgtcgt atcaatctga ataaattctt gatgtggatg tttacgtccg ccctgaggtg
    27181 gcacacttgc aacagttcct tctagaattt tcagcaaggc ttgttggaca ccttcgccgg
    27241 aaacgtcacg agtaatagat acattttcgc ttttacgcgc aattttatcg atctcatcaa
    27301 tatagatgat ccctttttct gcaagttcca cattgtaatc agcagattga agcaatttaa
    27361 gcaaaatgtt ttctacatct tcccccacat aaccagcttc tgtcaaactt gtcgcatctg
    27421 cgattgcaaa aggtacattc aatgtttttg ctaatgtttg tgctaaaaat gttttaccag
    27481 accctgtagg tccgatcaag cagatattgc ttttttgcag ttcaacatca tctgtgtttt
    27541 cttgtgcttg ctgattgaca cgtttatagt gattgtatac ggcaacagct aacgagcgtt
    27601 ttgcgcgatc ttgtccaatc acgtagttat taaatacttc aagaatttct agtggtttag
    27661 ggacttcagt gaattcgcgt acagcttctt cataaaattc ttcatcaatg atttctttac
    27721 ataggtcgat acattcatta cagatgtaga cccctggacc agctacgatc tttttcactt
    27781 cttcttgggt tttcccgcaa aaagaacagc gtacggtctc attgctactt ggattgtcat
    27841 acatggtctt caccccttaa cttgcaaaaa gtatgcaaga aactatcaaa gactttacag
    27901 tcttttacta tcataacata gtttagtaaa aagaaaaagt gtttaacaaa ggatcaactg
    27961 acctatacct ttgcattttt tactcttaac aaaaaataag gaactgtgac aaaatcgtgt
    28021 cacagtccct tatttcgaat aaacaatttt ctagaagtag cttcttcaga aatgatttat
    28081 tacttgattt ctattctttc gcttctgtcg ctgtacctgt gatcaattcg attgcttttt
    28141 tcattgtcac atcatgttcc agcatatctt ctgtcaacac gcgtttgatt tgttcgattg
    28201 gcatgttgta tgtttctgat aattcttgga tttctgcatt gatttcttct tctgttgctt
    28261 gaagattttc agcagctgca attgcttcga tcacaagatt tgttcttgtg cgcatttctg
    28321 cttcaccttc gaattgttta tgcaagtctt cttctgtaga accagttaat tgatagtaca
    28381 tttcaggaga gataccttga cgttgcatgt tgttcaagaa ttcgtccatt gaacgatgta
    28441 cttcatcatg aaccatcacg tgtggcaatt caacgatttc tgcgttttca acagccatac
    28501 ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc ttttgtttct actaattctt
    28561 tgcgatattt ttctttcaat tcgtctaatg ttgatacttc gtcatcaaca tcttttgcga
    28621 attcgtcatc taattcaggc acttcttttg cttttacttc atggattgtc actttgaaga
    28681 ctgcttcttt tccagcaaga tcttctgctt gataatcttc tgggaaagtt acatttacat
    28741 ctagactttc acctgattta tgaccaacta attgatcttc aaaacctgga atgaatgagc
    28801 ctgaacctaa ttcaagtgag taattttcgc cttttccgcc atcaaacgct tcgccatcta
    28861 caaagccttc gaagtcgatt actactgtat cgccattttc agctgcagca tcttctttga
    28921 taactaattc agcttgtgct tcttgttcac gtttgatacg tgcatccacg tcagcatctg
    28981 ttacttcacg gtcttgtttt tcaacaacta gatctttata ttgtcctaat tttacttctg
    29041 gtttcactgt tacttcagca gtgattaccc agtcttgacc tttttccatg ctttcaacat
    29101 cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt gacagcagct tcataagctt
    29161 ctggtaatac agcattcaat gcatcttcat ataaagcttc ttcaccgtac atacggttga
    29221 atacttgacg agaaactttc cctttacgga atccaggtac gttcaagctt tttttcactt
    29281 tattaaacgc agttgttaat cctttttgga tttcgtcttg cgcaatcgta aattttaaaa
    29341 caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat ttaattccct ccgagtttag
    29401 aaattcattt tatacatcaa aaacgtttca tgcatacctt taaatagtac actaaccctt
    29461 tcaaattgta aacatattga atggatagaa tccttgtttt tcataacatt tttatctttt
    29521 tcactgataa aatctaatgg aagaagatca tttgctgcaa aattttttct ctttttttta
    29581 aaaaatcttg tttttcatct tctgtacctt catacatcgt ttttcctgta cacgcctcta
    29641 cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt atcatagaca ggataaacaa
    29701 ttgcagattg caacgtgaat tcttctttta acacggaact aagcagcgca tcttcattcc
    29761 cgtaacgatc tgaaaataca cataattgct ggtaacttgc ctgctgctgc ggctttggca
    29821 gatcttttgg tatgactttg actaactttt cttcaatcgt caaatagaaa acaggatggc
    29881 attcgttcaa ttgcctaagt gtctccagta attttgccct tgctaaaata tggacctctg
    29941 gcgatacaat aagtttactc gctgtttgga taaactcatg ataaggaagc tgttcgatct
    30001 catcaataga cgcaagctga cgtgcaggtt ccaacgaagg aagttcctcc gatttattgt
    30061 ggagtgcctt gatcttctgc ctctccattt gtccggataa ttcttctgca aattggattt
    30121 tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt acttgctatc aattttctag
    30181 catataaaaa atcttgtgta ctgattaaca agtcaaaata aaattcagca aactcctcat
    30241 tttgaagata ctgtttttca tgcaagagtg cttgcttcaa tgcttctttt ttctcgtcca
    30301 actcgtaaag acaaaaaaca agcaggctat tagcctcaaa gctttctttc aactcgtaag
    30361 ctcgttgaaa taattctttt gcctgctgat attgctggtt tgctagtgct tgttgtgcca
    30421 ctcgcattaa attctcatat ttttctggaa aatcaatcgg cttcacgcag tgtcctcctc
    30481 ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa aaaagcatga caattaaaaa
    30541 taactgaagc accctctaaa agctagattt tagatctgac ttttagaagg tgctttcaac
    30601 tgttactgct ttttattgct catcagctgt tgacttcctg gttgaacgct ttgatcagat
    30661 tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag atgttgcttt ttctgattaa
    30721 agactgctgg aagataccct aacatcagca aaggaaattt tagaatgaac cggcaagcac
    30781 cttctctaac taaaacggtt gaccagctaa gttcttcttc atcaaagctg atgactcgaa
    30841 taccaaagat cattttccca atcgtttggc catgattgaa tttcgtcaat aaaataaaat
    30901 acgctagata aataagtaag gctgtgaatc cgtagagact cagataagaa tctgaaaaat
    30961 taagccaacc tagatttttg gcaataccaa gtgttaaacg cgtaatagct ccgatacaca
    31021 aaagatcgac caagaaggca aacattcgga taaaaaaacc tgcatagaaa tacttaggaa
    31081 aatcatttgc ttttggcggt tcatttcttg tgtccgtata ctttttccat ttacgttgtt
    31141 tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa agagctcagt accttttggg
    31201 aaaagttgga gtcaggtcct acagcaggct gctgttgctt atctttttct tgttccttat
    31261 tttcatttga aagctctgac atgcttactc acctccgtaa taatacattg cttttggtga
    31321 ttccgctgtc cctagattct caagaacgga taagatctga ctagtctcag aaggcttcaa
    31381 tccctggaat tctgctagtt tacttcctaa ccatgtacta gcaaacccag atggcgtgga
    31441 agaatattct actagttccg catcttcaag ctgttgttcc tttcgtatag cttttaaagc
    31501 atcttcagga aatcccaatt catcgactaa acctacttct tttgcttgga caccatcata
    31561 aatccttcca tcagcaattt ttttgacttc ttcttcagat ttattccgtc cctcgctgac
    31621 gattgctaca aaacgactat atgcgctgtc gatatatgct tgaagcaccg cttcttcttc
    31681 ttttgtctct ggtcgcgttc ctgaacccat atctttcaac gctccgcttt taactgttgt
    31741 atcttccaca cccagttttt ctagaagtcc tgaataattc aatcctgaca tgatcacacc
    31801 aattgagccg gtcactgttt cttctgttgc aaagatttta tctgcttgag cagaaatata
    31861 gtagccaccg ctagctgcca tgtttttcat gctgacatac attgggatat catgttcttt
    31921 acgaatcgta tccaatttct tagcgatttc cgcactttcg tagacaccgc caccaggaga
    31981 atttacttct aaaaagatac ctttgactgt tggatcttct tcaatcgcct tcagttgttc
    32041 cataaaattt ttatgattgt aaccttcaga agcaaataaa cctgaagacc ctcccgcagc
    32101 aatcgttcca tctactgata attttacgat tttttgcgta ctgtctccct cttccaacac
    32161 atttggagct agttcttcat ccccatataa ccaactgttc aatccctcaa tttgatcgtt
    32221 ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc acaaataatc ctgcagcaat
    32281 cagaactgct gcccaacgtc ttttattcat tctttctcct ttctaataca agttttttat
    32341 gacgatcaca gtttctgact gctcagaagt gtcgaaccgc tctgcttttt cttcccgcgc
    32401 atacccggaa aacgactctt ttgttaattc atcttctacc gtaaactcac gagcttgagc
    32461 aacttccaag gcatcaggtg cgatttcttg gatgctatcc cagccagtat ctaaaaatat
    32521 tttcgtatca tctgatacac cagctgatgc ttcgaatttg gacaattgtt tttttaacat
    32581 tcccagtgtc atgatctcaa tttttgaatc catttttact cctcctctat ccgattactt
    32641 tctattgtac cgaactcctg aaaaagatga tagataaaaa cccattattt tttgacataa
    32701 aaaggacatt tgacaaagaa tttgatatat accaatttat tttttattct ttttttgttg
    32761 acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc agataaaaaa caaaaaggag
    32821 gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt
    32881 tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat
    32941 gtctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt
    33001 atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta
    33061 acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt
    33121 atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat
    33181 ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca tacacgatgg
    33241 gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc
    33301 ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta
    33361 gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact
    33421 ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt
    33481 tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat
    33541 aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg
    33601 gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc
    33661 acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc
    33721 gaagcacaag tagatgcttc agatatcgag aaaggtacct atccttttta tatgctgaaa
    33781 gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac
    33841 aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat
    33901 atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac tttggaaaaa
    33961 ttgacacaga ttcctgttga ggttcattta gccagtgaat ttggctataa cacacctctt
    34021 ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc agacagccgc
    34081 caagtgctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac aaatgttgct
    34141 ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc
    34201 gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa
    34261 gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg
    34321 agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg
    34381 gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg tgctgactac
    34441 aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt
    34501 tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg
    34561 atcggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc
    34621 gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac
    34681 cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact
    34741 caattacttg catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat
    34801 ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac
    34861 actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct
    34921 ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt aggcgcttct
    34981 gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat
    35041 cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg
    35101 tttttatcac ttacgcggat attcactaat ttttgcggat agattgttac ctctgctgca
    35161 agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc
    35221 atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca
    35281 aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg
    35341 acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc
    35401 gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc acagataaac
    35461 atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca
    35521 tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt
    35581 tctggatggg tagagcctac gccgtcattg atatttaacc cgtctggatt tgtgcccatt
    35641 gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg
    35701 tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg
    35761 aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct
    35821 gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg
    35881 tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga
    35941 ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca
    36001 acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct
    36061 gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg
    36121 cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc
    36181 ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca
    36241 aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc
    36301 tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc
    36361 ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag
    36421 cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag
    36481 agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga
    36541 tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg
    36601 agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc
    36661 ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg
    36721 ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca
    36781 ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct
    36841 gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta
    36901 ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac
    36961 taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt
    37021 ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga
    37081 tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacttca tgtgttcctt
    37141 cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg
    37201 cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat
    37261 ggatatagta gcgctttgaa tcataagaaa tcttgatcgg cacccccgta actgtttgat
    37321 tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca
    37381 gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg
    37441 agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt
    37501 cttgtttttt gttttctctt cagcaattag ttctctacgc aagattgcca aatattcttg
    37561 ctgggataag ccagccatca gttcattatc taacgtgatg ctgacgtccc cggtttcttc
    37621 agagacaacg atggtaatcg cgtcactgac ttcgcttata ccgacagctg cccggtgtcg
    37681 agtcccgaat tctttaggga tcaacatact ttctgataga ggaagatagg cgcttgctac
    37741 tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa
    37801 gatattgatc agcagctccc cagtgatatc tgcatctaaa gcaatccctg tctcaatata
    37861 ctcatctaat ccagtatgac gttcgatagt gatcaacgca ccgatttttc gcttggacat
    37921 atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt
    37981 tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg
    38041 aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa
    38101 ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt
    38161 cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata caagatacca
    38221 gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa
    38281 catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct
    38341 ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgata
    38401 tgctcgtatt tttcttgaga aaaacgcata attttcaaga aaattgcaag tatgcgtttt
    38461 atttttctct gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt
    38521 tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca
    38581 gctcttctag cagctcttct ttcttctgct tcaaggtccg tcgttttttc ttttctagtc
    38641 ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt ttctaattct
    38701 cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cgtggacagt cttttcaaat
    38761 aaagcttttg caagatttgg atttgcgtta tctagcgagg aaaagcgaac ttgtttcctc
    38821 atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat tgggttcttg
    38881 tttttttcga ccattgtagg gttatatcga tataatgacc aataaccaga agaaacagct
    38941 tcttttgctt ctttaagcga ttctttcatt ccgccaagca agccatgtgt gatacaaggc
    39001 gtataggcaa taatcagaga aggaccagga tacttttctg cttcttcaaa tgccttgatc
    39061 gtttgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc atatgtcatc
    39121 gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa tttagcgatt
    39181 gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac ttcgttatcc
    39241 aaaacaagga tattcacgtc tgctccgctg gcaagtacat gatcgatccc gccaaaacca
    39301 atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac gaatagatcc
    39361 tttctcttgt atatatcctc cagttcaggg aaaattgatc gttcgctttc gatcgcagct
    39421 tgcagttttg ctgctctttg ctgcgttcct tcactctcaa acatgtgtac taaccagtct
    39481 tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat tgaacttgcc
    39541 aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc agcattatct
    39601 tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt ataaggagaa
    39661 gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat catcattcga
    39721 tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc tgagcaagcg
    39781 cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt ctttcctttg
    39841 actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc ttgttctttc
    39901 tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc ctctacacac
    39961 agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac gccgtctgcg
    40021 cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc ttcatctgct
    40081 aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca catcgtacag
    40141 cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat agaagccgtt
    40201 cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac agagagttga
    40261 tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt gttcctttta
    40321 ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac ttgatgaagc
    40381 agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat tgatttatgt
    40441 ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa aggaatgatg
    40501 tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc caaaccgact
    40561 tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc agccaagtaa
    40621 cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag agtattcaat
    40681 aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag gtaagctggt
    40741 gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat cggcgtatct
    40801 ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata ataaaaatag
    40861 ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt atttgctcca
    40921 actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg ttctgtcaaa
    40981 tccagacttt cctttggttc caaagattga taagtcacat catccacgat ccctatggta
    41041 aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc tgagatttgg
    41101 tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc cgggcgaagt
    41161 tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc tgcccctggt
    41221 tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt ttgcacgaat
    41281 gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt ccgtccgttt
    41341 gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat agaaacaatg
    41401 acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt tcctcgtaat
    41461 cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata ataggggttg
    41521 atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact aacagtcgga
    41581 tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt gatcagagga
    41641 gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt acggaatcca
    41701 tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag caagatgggc aacaggtgct
    41761 aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt ttgtcttgca
    41821 gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac tgcccgacta
    41881 gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt cggtatcatc
    41941 aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt caatgatcca
    42001 tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac tggttgaccg
    42061 aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat ggtcgaactg
    42121 ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata tgcggctgcc
    42181 gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc atattctctc
    42241 ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt tataaaaata
    42301 ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc taaatcgttg
    42361 cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac atggatatat
    42421 cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt tcatcgaatg
    42481 ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga caatggtttg
    42541 aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag cgagtgatca
    42601 aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac cggtatctca
    42661 caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat actcaagacg
    42721 agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa ctaaagctga
    42781 cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag caagcacata
    42841 ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct atttcaatag
    42901 aggaattaaa agaattgatc caaacaaaat aaaaaaataa
//
LOCUS       DADOZM010000009        40498 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.009, whole genome shotgun sequence.
ACCESSION   DADOZM010000009 DADOZM010000000
VERSION     DADOZM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 40498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.009"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(7..963)
                     /locus_tag="J8I47_000405"
     CDS             complement(7..963)
                     /locus_tag="J8I47_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6321871.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="J8I47_000406"
     CDS             1137..1697
                     /locus_tag="J8I47_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321872.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="J8I47_000407"
     CDS             1690..2514
                     /locus_tag="J8I47_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAZ6321873.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="J8I47_000408"
     CDS             2524..3141
                     /locus_tag="J8I47_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAZ6321874.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="J8I47_000409"
     CDS             3159..3842
                     /locus_tag="J8I47_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAZ6321875.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5760
                     /locus_tag="J8I47_000410"
                     /pseudo
     CDS             4525..5760
                     /locus_tag="J8I47_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            5768..6655
                     /locus_tag="J8I47_000411"
     CDS             5768..6655
                     /locus_tag="J8I47_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6321876.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6672..7508
                     /locus_tag="J8I47_000412"
     CDS             6672..7508
                     /locus_tag="J8I47_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6321877.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7511..8134
                     /locus_tag="J8I47_000413"
     CDS             7511..8134
                     /locus_tag="J8I47_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321878.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8131..9423
                     /locus_tag="J8I47_000414"
     CDS             8131..9423
                     /locus_tag="J8I47_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6321879.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9782..9879
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9995..10576
                     /locus_tag="J8I47_000415"
     CDS             9995..10576
                     /locus_tag="J8I47_000415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAZ6321880.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10579..11886
                     /locus_tag="J8I47_000416"
     CDS             10579..11886
                     /locus_tag="J8I47_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAZ6321881.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12179..12667
                     /gene="purE"
                     /locus_tag="J8I47_000417"
     CDS             12179..12667
                     /gene="purE"
                     /locus_tag="J8I47_000417"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAZ6321882.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12651..13784
                     /gene="purK"
                     /locus_tag="J8I47_000418"
     CDS             12651..13784
                     /gene="purK"
                     /locus_tag="J8I47_000418"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAZ6321883.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13797..15092
                     /gene="purB"
                     /locus_tag="J8I47_000419"
     CDS             13797..15092
                     /gene="purB"
                     /locus_tag="J8I47_000419"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAZ6321884.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15272..15649)
                     /locus_tag="J8I47_000420"
     CDS             complement(15272..15649)
                     /locus_tag="J8I47_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321885.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            15905..16633
                     /locus_tag="J8I47_000421"
     CDS             15905..16633
                     /locus_tag="J8I47_000421"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAZ6321886.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16633..16887
                     /gene="purS"
                     /locus_tag="J8I47_000422"
     CDS             16633..16887
                     /gene="purS"
                     /locus_tag="J8I47_000422"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAZ6321887.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            16889..17560
                     /gene="purQ"
                     /locus_tag="J8I47_000423"
     CDS             16889..17560
                     /gene="purQ"
                     /locus_tag="J8I47_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAZ6321888.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17561..19783
                     /gene="purL"
                     /locus_tag="J8I47_000424"
     CDS             17561..19783
                     /gene="purL"
                     /locus_tag="J8I47_000424"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAZ6321889.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19768..21207
                     /locus_tag="J8I47_000425"
     CDS             19768..21207
                     /locus_tag="J8I47_000425"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAZ6321890.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21239..22282
                     /locus_tag="J8I47_000426"
     CDS             21239..22282
                     /locus_tag="J8I47_000426"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAZ6321891.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22279..22857
                     /gene="purN"
                     /locus_tag="J8I47_000427"
     CDS             22279..22857
                     /gene="purN"
                     /locus_tag="J8I47_000427"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAZ6321892.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            22854..24389
                     /gene="purH"
                     /locus_tag="J8I47_000428"
     CDS             22854..24389
                     /gene="purH"
                     /locus_tag="J8I47_000428"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAZ6321893.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24405..25679
                     /gene="purD"
                     /locus_tag="J8I47_000429"
     CDS             24405..25679
                     /gene="purD"
                     /locus_tag="J8I47_000429"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAZ6321894.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25801..26715
                     /locus_tag="J8I47_000430"
     CDS             25801..26715
                     /locus_tag="J8I47_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAZ6321895.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(26848..28413)
                     /locus_tag="J8I47_000431"
     CDS             complement(26848..28413)
                     /locus_tag="J8I47_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ6321896.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28406..29857)
                     /locus_tag="J8I47_000432"
     CDS             complement(28406..29857)
                     /locus_tag="J8I47_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6321897.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(29871..30887)
                     /locus_tag="J8I47_000433"
     CDS             complement(29871..30887)
                     /locus_tag="J8I47_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6321898.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31107..32195
                     /locus_tag="J8I47_000434"
     CDS             31107..32195
                     /locus_tag="J8I47_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ6321899.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32249..33775
                     /locus_tag="J8I47_000435"
     CDS             32249..33775
                     /locus_tag="J8I47_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321900.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33777..34982
                     /locus_tag="J8I47_000436"
     CDS             33777..34982
                     /locus_tag="J8I47_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6321901.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35239..36216
                     /gene="guaC"
                     /locus_tag="J8I47_000437"
     CDS             35239..36216
                     /gene="guaC"
                     /locus_tag="J8I47_000437"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAZ6321902.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36480..37904
                     /locus_tag="J8I47_000438"
     CDS             36480..37904
                     /locus_tag="J8I47_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAZ6321903.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38365..39474
                     /gene="pdhA"
                     /locus_tag="J8I47_000439"
     CDS             38365..39474
                     /gene="pdhA"
                     /locus_tag="J8I47_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAZ6321904.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39477..40454
                     /locus_tag="J8I47_000440"
     CDS             39477..40454
                     /locus_tag="J8I47_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAZ6321905.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        12931 a         6909 c         9066 g        11592 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 ggaaggcatg aaaaaagaaa aactaaaacg gttcttggat caagacaatg tggtcggtta
     5821 cgtatttgct tctccattca tcctaggatt tctattgata acagtgatac cgatgatctt
     5881 gtcactttat tattcgttca ccgattatcg tttgggaaat ccagtctcat ggatcggctt
     5941 ggataattat ataaagatgc tgagtgatgc ccgttttttg aactccatcc gagtcacttt
     6001 tctctatgta tttgcatctg tacctgtgaa actgatcttt ggtctattga ttgcttatct
     6061 tttaacgagg aatatcaaag gaacgacttt ctttcgttct ttgtattatg tgcctagttt
     6121 gatcggtggc agtatttcta tcgctctagt ttggcgggag ttgttttcta aaaacgggtt
     6181 agtgaatatt ttcctggaac gattaggcgc acctgcgctt tcctggttta gcgatcaaaa
     6241 actggtcatg atccctcttg ttttgatgtc tgcttggcaa ttcggttcgg caatgatcat
     6301 ctttgctgca ggtatcaaag agatcccagg ctcgtattat gaagcggcag agatcgatgg
     6361 agcgaacaaa tggcagacgt tttacaagat cacgttacct cttctttcgc cgatcatttt
     6421 gtataacttg gttatgcaga caatcgcggc gtttatgact tttacgcagg catttatcat
     6481 tacccaaggt ggtccgaatg atgccacgaa cttttatgct ctttatgtgt ataaccaagc
     6541 tttttctttt aattctatgg ggtatgctag tgctatttcg tggatcatgc tgttgattat
     6601 gggagctgtc acctttctga ttttcaaatc atcaaaaaaa tgggtcgttg tgtgaatgag
     6661 aggaggaaga aatgaataat acagtaacga aatggtttca atacggtttt attttgctat
     6721 taggtctagt catgatttat ccggtcttat ggatgatcgc aggttctttt aaaacggatc
     6781 aagagattat gagtggttcg ctgaatttga ttccagagac ttttcaatgg gaaaattacg
     6841 caaaaggatg ggcggggttt gctggaattt cattttttac tttctttagg aattcgtttg
     6901 tcatctctgc agtttcgacg atagggacag ttctgagctc gacttgtatt gcttacgcat
     6961 tatcgagaat cgatttcaaa ggaaaaagat tctggttcgt cgtcatgtta gtaacgatga
     7021 tgattcctgc acaagtgatt ttgattccgc agtttattat ttacaatcgg ctgaatttgg
     7081 ttgggacgta tgtgccgctg attttaccac atttttttgg acaagcattt ttcatttacc
     7141 agatcatgca attcatggtc agcatcccca aagagttaga tgaatcagcg attattgacg
     7201 gatgcagcaa gtatagtgtc tttaccaaaa ttatttttcc tttgctgaaa ccttcgatca
     7261 tcacgacgat catcatccag ttttattgga aatgggacga cttcatggga ccattgattt
     7321 atttaaacaa gccgagatct tacacggtat cgattgccat caaattattt gcagacgctg
     7381 gatctagtac gacttacgcg agtatgtttg cgatgtctac gttgtcgctg ctaccggttt
     7441 ttctgatttt cttattcttc aataaatatc ttgtccaggg gatcagtaca agcggtctga
     7501 agggctgaat atgaaaaagc caatagaaga gatgtttctt tacaagctca actactatgt
     7561 ctgtcggatt tttttaacca gtcttatcag taatgctctt ttcgctttag ttctaggatg
     7621 tttgttcact ttaggattag tgctgtctgc tcgtgttgtc tatctgattt attttttgat
     7681 gatgtatcct tcttattatc tatctgtcca ttacttggca gaaaaaaagg tatgttggag
     7741 gaaagggaaa atcaaacgga tgtgtttcat cattacgttg tttcagctgt ttgtcattat
     7801 ttttgcagtt gattggtatt tattagtggg aaagattgga gcttggtatc tgattccttt
     7861 tctattgtgt ctgttgcttt ttgtatccca tagttttttt agtacgctaa ttgatatctg
     7921 caaagaagat aaagcagata gtcttgttta tttgatcaaa aggaatatct ggctgttgtc
     7981 aaaaaacgtc aaggggtttc tgtggtattt atgccttttg tatggactgt tctggtgtat
     8041 acagcctgat tttttactca ctgcattact gtatccaggg ctgccatttg tcacagtatt
     8101 ttgcttaaaa gaaaaggaga atgactttaa atgaagaaaa aatatgtaca agtcggactt
     8161 ggtgggagag cgcgtttttt ttatgaggca attgcctcta cgtatcatca aacttgtgaa
     8221 ctagtcggtt tttgtgatgt atctcaaaca aggatggatt atgcaaatcg gatacttgtg
     8281 aacaagtatg gacagaaaaa agtaaagaca tgtatgccat cacaatttga gcaactactg
     8341 gatgacgtac ggccggctga agtcattatt acgtcagtag atcgaacgca tcatgattat
     8401 atcattcgtg caatgaaaaa aggatatgat gttatttgcg aaaaacctct gactatcgat
     8461 gaggaaaaag ctcaagaaat aataacggtc caaaaacaga cgggaagaag cctgcgagtc
     8521 acttttaatt atcgttatgc accgtatcac acgaagataa aggaactgat agaaagtgat
     8581 gtgattggtg atgtgttttc tatccacttc gaatggctgt taaatacgca acacggggca
     8641 gattatttca gaagatggca tcgaaataag cacaatagcg gcgggcttct tgttcataaa
     8701 tctacccatc attttgatct agtgaatttt tggttgaaca gccgtcccca aaaggtattt
     8761 gcattcggtg gattaaactt ttatgggatg cataatgcgg agaaacgcgg agtagaaata
     8821 ttttacagtc gatgctatgg aagcgaacta gctaaagctg atccatttgc tatagacctg
     8881 tcagccaatg aggaattgcg agaaatgtat ttagaagcag aaaaagacag tggatatatt
     8941 cgtgaccgca gtgtattttc cgatgatatc tctattgaag atacactagg cgtaacggtc
     9001 aaatatgaaa ctggtgcgat tttgacgtat tcgctaaacg cgtatatgcc gtgggaagga
     9061 ttcaatgttt ctttcaatgg cagcaaagga cggatcgaaa tgaaagtgat cgaaaagtct
     9121 tacatcaatg caggtggaga taaaaaggat gaaggagcag ccgttcttca gtccatccat
     9181 gtctatccga tgttcggtga gccatatgag gtaatcgtcg aacaagaagc gggcggacat
     9241 ggcggaggag atccgcgttt attagatgat ctatttggtg aagaaaaaga agaagatccg
     9301 tggaatcggg cagcaactca cgtagatggt attttgtcga ttttgacagg aatcgcagca
     9361 aatcattcta ttgcttcagg aaaagcagta gtgattgacg agcttgtcag cttaaaagaa
     9421 taagaataga tttacggaaa ttacttcccg tagtttataa aatcttgcct tattgccgat
     9481 aggctgtctt ttgaacacta tttattcgga aaaaggggct atgacatgct ttttgtcatg
     9541 ccttcttttt atgtgggctg cttttttaga taggagaaaa taaaaataac tatttaaacc
     9601 cgaacaaaaa ggaataatgc attaaaaatc attagatttt acaaaaaaat aagtaaacca
     9661 cgttgtcagt ttcagaaagt agtgttagac tgttttcaat atttgaaaca acatttttta
     9721 ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga atttacacta
     9781 ttattagttt tacataccta cttatatatc gtcataatat ggatgacagt ttctagccag
     9841 taccgtaaat gctggactat aagtaaaaga ttggctattt cacccttctt ttcttatgat
     9901 aaagtggagg gtgtttttct ttaacagaaa catagtaggt ttttgcacaa aagcggttat
     9961 ttcggtatta ataaatgata aaggagcaaa taaagtgaaa gaattagttg aacgtattca
    10021 aaaagacgga cgtatattag gcgagggcgt attaaaagtc gacagcttcg tcactcacca
    10081 agtagaccct gtattgatgg aacaaatggg aaaacggttt gcagaagtat ttaaagaaca
    10141 aggaatcacg aaagttgtga ccattgaagc ttctgggatc gcaccagctt tatttgctgc
    10201 aagagaatta gatgttccaa tgatcttcgc tagaaaagca aaaagtttga cgatggatga
    10261 agaattattg acagcttctg tttattcttt tacgaaacaa gtgacaagta cgatttctat
    10321 ttctcgtaaa tttttatctt cagaagacaa agtattgatt atcgacgatt tcttggcaaa
    10381 cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc aagtagaagg
    10441 aatcgggatc gtcatcgaaa agtctttcca agatggacgt cagttacttg aagaaatggg
    10501 attgagagtc gtttctcttg cgcgtattgc ttccttgacg aatggacaag ttgagtttct
    10561 tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc ggttttaggg
    10621 ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg tgcctctttt gatcggtaca
    10681 ggactaggct ttaatcaaga acagatgact tatttgattt caattgatat ctttatgtgt
    10741 ggattagcga cattattgca attgacagtt aaccgatttt tcggaattgg cttgcctgtc
    10801 gttttaggat gtgcgatcca agcagtagct ccactgatct tgatcggaac caatgaagga
    10861 gtaggagcta tctacggatc tattatcgct tctgggatct ttgttgtgtt agtttctggt
    10921 gtcttttcta aaattaaaaa actattccct cctatagtaa caggaacggt tatcaccgtt
    10981 attggtttga ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa tgcaacagca
    11041 gctgattttg gtgacaaaac aaatctgcta cttgcatttg tgacgattct cctaattgta
    11101 ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg cggtattgat tggtttagtc
    11161 ggaggaagca tcttagctgc tttcatggga atgattgatc tgagcgcgat tggagatgcg
    11221 ccgttgttcc atgttccaca acccttttat tttggtaaac caactttcga cgtatggtcg
    11281 attttactaa tgatcatcat ttccatggtc agcatggtag aatcaacagg tgtatatttt
    11341 gcacttggtg acattactgg gaaaaatgtt ggagaagaag aattgaaaaa gggatatcgt
    11401 gcagaaggac ttgccgttat cttaggcggt attttcaata cattccctta tactggcttt
    11461 tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa caagaaaacc catctatttc
    11521 tctgcattct tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt agcacaaatc
    11581 atccctgagc cggtacttgg tggaggaatg ttagtcatgt tcgggatggt ggcagtccaa
    11641 ggaatccgta tgctgttgga agttgatttc agcaatgata aaaatctatt gattgttgct
    11701 gtttctgttg gatttgggct gggtttcaat gttacgccga cattattcag tcaaatgccg
    11761 caaacattgc aaatgttcac tggaaatggc atcgtaatga gtagcattac ggctatcata
    11821 ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa tattggaaga agccttcaag
    11881 aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg ttagagaaac
    11941 aaacggaaaa atagttcctc gtattttgcc gtttgtttga gctaataccg agggtcagct
    12001 gattgaacac cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac attccttttt
    12061 ttcatcttta ataaacacga acaataaaat gtttttcttt gttaacattc gaaatttctt
    12121 tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa aaactgggag gaaaaacaat
    12181 gacagcaaaa atctctgtga ttatggggag tacttcagat tgggaaacaa tgaaagaagc
    12241 atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg
    12301 aaccccagat ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat
    12361 tattgcagga gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt
    12421 gccggttatc ggtgtaccgg tccaatcacg aacattaaat ggtttggatt cgcttctttc
    12481 gattgtccaa atgcctggag gagtacctgt agctactaca gcaatcggaa aagcgggagc
    12541 aattaatgct ggactacttg cgatacagat gctttccatg tatgatttag aattggaggc
    12601 taaattggct gaaagacgtt caatgttggc taaaactgtt atagaaagta gtgatcaact
    12661 tggttaaacc tttgttgcct ggttcaacga tcgggatcgt cggtggcggt caattaggtc
    12721 gtatgatggc gattagtgca aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga
    12781 gcgattgtcc agccgcacaa gttgcagatt ggcacattga aagtgtctat gatgacacct
    12841 ttgcgctgaa agagcttgcc agaagaacag atgtcattac ctatgaattc gaaaacgtca
    12901 gtgtagaggc gttaaatgcg attttaccca tgtcatttat tcctcaaggg acagacttgc
    12961 ttgcgattac gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat aacatcgtga
    13021 ttgctcctta tgcaacgatc gtcagtccga cggatatcca agatgcgatt gacgggatcg
    13081 gctatccttg tgttttgaaa accactcgag gcggctatga tggcaagggg cagtatgttt
    13141 tgaaaagtcg ggcggatcta gctcctgcca tggacctttt aagagaagga acctgtgttt
    13201 tagaagcatg gatcccgttt gaaaaagaga tttcgatcat ggtggcagga aatggtcaag
    13261 gagatttcac gacttttccg gtcgtggaaa acatccacca caacaatatt cttcatgaaa
    13321 cgattgctcc agccgcgatt gatcaggatg tgatcgagga ggctgagcgt attgcccgtg
    13381 tgattgcaga agcagtgagc ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa
    13441 caggcgggct atatgtgaat gaattagctc ctcgtcctca caattctgga cattattcta
    13501 ttgaagcttg ttctatgagt cagttcgatg cccatattcg aggtatttgt ggttggccat
    13561 taggagaaaa agtacgtttg ataagcaatg cagtaatggt caatgttttg ggagaacaac
    13621 tgctggaaag ctatcgcttg attgagaata agccagactg gcaattccat ttctatggaa
    13681 aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca gaagacatcc
    13741 agcaaacgct ggaagaaatc aaacaaacga atatttggga ttagaggaga aaaaacatgt
    13801 tagaacgtta tacacgccct gaaatgggga atatctggac ggaagagaac aaataccgtg
    13861 cgtggctcga agtcgagatt ttagcagatg aagcttgggc agaacttggt gaaatcccgc
    13921 aagaagatgt gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg atcttggaaa
    13981 tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg agcggtttct gaaacattgg
    14041 gagaagaaag aaaatgggtc cattatggtc taacaagtac ggatgtcgta gatactgctt
    14101 atggctatct catcaagcaa gcaaatgata ttttacggga agacttgaaa cgttttacgg
    14161 aaatcattgg cgaaaaagca aaagaacaca aatatacagt gatgatgggt cggacacacg
    14221 gtgtccatgc tgaaccaact acttttggat tgaaacttgc cacatggtat tcagaaatga
    14281 aacggaacat cgaacgattt gaacatgcgg caaaaggagt agaagcagga aaaatctcag
    14341 gagcagtggg aacgttcgca aatatcccgc catttattga agagtatgta tgtaaacaat
    14401 tggggatccg tccacaagaa atctctactc aagttctccc tcgggatcta catgctgaat
    14461 atttctcagc gatggcgctt attgctacaa gtattgaacg ttttgcaaca gagatacgcg
    14521 gattgcaaaa atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt
    14581 cttctgctat gccgcacaaa aggaatccga tcggttcaga aaatatggct ggtctagcca
    14641 gagtggtgcg cggacacatg gtcactgcct atgaaaatgt cacattatgg catgaaagag
    14701 atatttctca ttcttcagca gaacgaatca tcttgcctga taccacgatt ttattgaatt
    14761 acatgttaaa tcgttttggc aatatcgtta aaaatctaac ggtctttcct gaaaacatga
    14821 agcggaatat gtctgcaaca tttggtttga tttatagcca gcgcgttatg ctgaaattga
    14881 tcgataaggg aatgacaaga gaagaagctt atgatcttgt tcagccaaaa actgcgtacg
    14941 cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga agatccaacc atcacatcgg
    15001 tattatctaa agaagaaata gacgatgcct ttgattatca tcatcatttg aaaaacgttg
    15061 acgtgatatt caaacgagta ggtttggaat aaagaagagt gagttgcaaa attcaattaa
    15121 aatagaatag caaaaataac caaaaaagaa gcagatccgt tagcggttct gcttctttta
    15181 gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct acctctttac
    15241 cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg ctattataac
    15301 taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat tccatctctt
    15361 gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt tcatgaatct
    15421 gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt ttacggcatt
    15481 cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta gtatctttat
    15541 tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa cgttcttcag
    15601 ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc ggcaagtgcc
    15661 tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt aagtataaat
    15721 gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac attaaaagaa
    15781 tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta
    15841 aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac
    15901 gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc
    15961 tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag
    16021 gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg
    16081 ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca
    16141 attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg
    16201 aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga
    16261 attctactat aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt
    16321 cttacaactt gcaaaaccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa
    16381 tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga
    16441 atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg
    16501 attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg
    16561 tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa
    16621 ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat
    16681 cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat
    16741 atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca
    16801 gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat
    16861 gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc
    16921 aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt
    16981 tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt
    17041 ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga
    17101 agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat
    17161 attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat
    17221 ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc
    17281 cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga
    17341 aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa
    17401 cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat
    17461 gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt
    17521 tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc
    17581 gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga
    17641 atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact
    17701 gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa
    17761 atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga
    17821 catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt
    17881 ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat
    17941 gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag
    18001 aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg
    18061 cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt
    18121 caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg
    18181 cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc
    18241 aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt
    18301 aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca
    18361 ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc
    18421 ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag
    18481 agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat
    18541 ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga
    18601 tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga
    18661 agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga
    18721 aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat
    18781 cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg
    18841 ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag
    18901 cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg
    18961 caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc
    19021 tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta
    19081 tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc
    19141 tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga
    19201 aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga
    19261 tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg
    19321 taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga
    19381 aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac
    19441 agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat
    19501 tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc
    19561 ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa
    19621 taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga
    19681 tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc
    19741 aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa
    19801 tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc
    19861 catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag
    19921 cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg
    19981 cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga
    20041 agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg
    20101 cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg
    20161 atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa
    20221 acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat
    20281 ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta
    20341 gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa
    20401 gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac
    20461 gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa
    20521 tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg
    20581 aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc
    20641 gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat
    20701 gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag
    20761 ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa
    20821 aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat
    20881 cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa
    20941 tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac
    21001 acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag
    21061 ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct
    21121 tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca
    21181 ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat
    21241 ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg
    21301 gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt
    21361 cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac
    21421 cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg
    21481 gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata
    21541 tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa
    21601 aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga
    21661 aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc
    21721 tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat
    21781 agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca
    21841 gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt
    21901 gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat
    21961 aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc
    22021 agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg
    22081 tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat
    22141 cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg aaattgagca
    22201 agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt
    22261 gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt
    22321 caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt
    22381 tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat
    22441 tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa
    22501 ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg
    22561 gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc
    22621 ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc
    22681 acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg
    22741 agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat
    22801 cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa
    22861 gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg
    22921 aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga
    22981 tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca
    23041 aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata
    23101 tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc
    23161 ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg
    23221 atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg
    23281 ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt
    23341 cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg
    23401 cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac
    23461 ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct
    23521 tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga
    23581 aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat
    23641 atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat
    23701 ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg
    23761 ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt
    23821 ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa
    23881 agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag
    23941 aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg
    24001 aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag
    24061 cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac
    24121 gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga
    24181 ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa
    24241 tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag
    24301 gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct
    24361 ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga
    24421 tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg
    24481 atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg
    24541 ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt
    24601 ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg
    24661 aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt
    24721 ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat
    24781 gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg
    24841 acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg
    24901 cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag
    24961 aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt
    25021 atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata
    25081 caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag
    25141 ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca
    25201 cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca
    25261 atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg
    25321 cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag
    25381 gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa
    25441 tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa
    25501 ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga
    25561 cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa
    25621 cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa
    25681 tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga
    25741 agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag
    25801 atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt
    25861 atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc
    25921 attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca
    25981 gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga
    26041 atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa
    26101 agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca
    26161 ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt
    26221 cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt
    26281 cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat
    26341 caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt
    26401 gattattccc gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt
    26461 tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa
    26521 ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat
    26581 ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct
    26641 caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga
    26701 aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag
    26761 aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa
    26821 agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt
    26881 ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg
    26941 gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa
    27001 tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga
    27061 ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt
    27121 taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat
    27181 tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat
    27241 ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga
    27301 tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag
    27361 cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt
    27421 caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag
    27481 gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct
    27541 gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga
    27601 tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt
    27661 ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac
    27721 attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa
    27781 gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc
    27841 ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa
    27901 agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa
    27961 caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt
    28021 ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct
    28081 acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata
    28141 atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc
    28201 ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc
    28261 gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata
    28321 agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag
    28381 gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag
    28441 atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag
    28501 gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc
    28561 cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt
    28621 ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc
    28681 atgcggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat
    28741 tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta
    28801 tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact agataatcat
    28861 tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac
    28921 gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga
    28981 tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca
    29041 atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact
    29101 ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt
    29161 ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca
    29221 gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga
    29281 aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa
    29341 gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat
    29401 ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa
    29461 aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg
    29521 tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgatct
    29581 ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca tctacggtta
    29641 cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa
    29701 ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat
    29761 ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt
    29821 gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt
    29881 ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct
    29941 ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca
    30001 taccagccga catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc
    30061 tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt
    30121 gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat
    30181 acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc
    30241 atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg
    30301 gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt
    30361 agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc
    30421 tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa
    30481 ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca
    30541 agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct
    30601 tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg
    30661 atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca
    30721 agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc
    30781 gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt
    30841 tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc
    30901 atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc
    30961 aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa
    31021 tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt
    31081 gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta
    31141 tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg
    31201 atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg
    31261 atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg
    31321 gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag
    31381 atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa
    31441 aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg
    31501 attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag
    31561 agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc
    31621 ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata
    31681 tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc
    31741 ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata
    31801 ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt
    31861 cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac
    31921 aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct
    31981 ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg
    32041 gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta
    32101 cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca
    32161 ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga
    32221 gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat
    32281 caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg
    32341 ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt
    32401 gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg
    32461 tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt
    32521 agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga
    32581 aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt
    32641 cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact
    32701 agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac
    32761 cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga
    32821 gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga
    32881 tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag
    32941 cgaagagcat attattcgcg ggatggtggg cagagattta gttgatcgtt atcctgaaag
    33001 aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct atcaccctct
    33061 tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt
    33121 aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca
    33181 ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa
    33241 gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt
    33301 aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat
    33361 aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa
    33421 aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa
    33481 tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgga
    33541 tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga ttatcgaaga
    33601 aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg aaatattagg
    33661 gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag tctcgcgaga
    33721 agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg
    33781 aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa agtagagcag
    33841 ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt attgcgtttc
    33901 aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt gttctgcaaa
    33961 atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta ggtcatgtgg
    34021 atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg atgatggtca
    34081 atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc gggctgatcg
    34141 gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc gtgactttag
    34201 caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa tctctagcac
    34261 cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta agcgaaggaa
    34321 gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg ttagttgttg
    34381 gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa tctccaacag
    34441 ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact tatatctttg
    34501 cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt gcagcatatg
    34561 ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa
    34621 aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca
    34681 tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat gcggcaacac
    34741 ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt ggtggtgctt
    34801 cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg
    34861 tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag gcaatcaaag
    34921 gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa cgaacaacgt
    34981 aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac agtctttttt
    35041 tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata tatgtttttt
    35101 taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga gttaaattac
    35161 atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc tggatgtgaa
    35221 catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt aattcctaat
    35281 aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg taaacataca
    35341 ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac aatcgcagaa
    35401 ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc agctcgaatt
    35461 ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag
    35521 aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc tgattatatc
    35581 actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca gcatattaaa
    35641 aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt
    35701 gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg aaaagtatgt
    35761 attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc tcttagatgg
    35821 tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac acatggcgat
    35881 attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt gtttgccggt
    35941 cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt
    36001 ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg
    36061 atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga ccttcaatca
    36121 tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga ctatgtcatc
    36181 gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat attaggatgt
    36241 tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata gttctttata
    36301 tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca ttattcagaa
    36361 taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa aagtttttct
    36421 tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa gagaagctta
    36481 tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg gaacagatta
    36541 tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca gaacgaaaac
    36601 tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa gagcttaggg
    36661 agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag actgaatggg
    36721 ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt tatgagcaga
    36781 cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca tcgtttcttg
    36841 atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt ccttcttatt
    36901 ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat actccgttac
    36961 gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc cccaaccagc
    37021 ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg attctttcag
    37081 ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct cttttcaaag
    37141 cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac ttagaaaaac
    37201 tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg aattttcaaa
    37261 accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac
    37321 agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt ctaaaccaag
    37381 aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc ggttcgttat
    37441 ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag acttttatct
    37501 cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt acacaaatca
    37561 cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta agaaaaaagt
    37621 tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt ttcaagcttc
    37681 attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa ctagaccccg
    37741 aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta ctttttgggc
    37801 aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa ataccgttgt
    37861 tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac aaagtaaata
    37921 gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt ttcggaaaaa
    37981 gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta
    38041 aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat
    38101 ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aaaataaaac gctttcctta
    38161 ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg
    38221 aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt aatgaagacg
    38281 gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat
    38341 agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca
    38401 ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac
    38461 gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga attaatgact
    38521 catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc
    38581 ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca
    38641 tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat
    38701 ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat
    38761 ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca
    38821 gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact
    38881 ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac
    38941 catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa
    39001 aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt
    39061 caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg
    39121 gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat
    39181 acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag
    39241 aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa
    39301 gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa
    39361 gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct
    39421 ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg
    39481 cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg
    39541 agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg
    39601 aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg
    39661 gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc
    39721 aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa
    39781 gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg
    39841 gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg
    39901 gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta
    39961 ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag
    40021 aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg
    40081 gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag
    40141 acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat
    40201 tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag
    40261 aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta
    40321 tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg
    40381 gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa
    40441 tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DADOZM010000010        39177 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.010, whole genome shotgun sequence.
ACCESSION   DADOZM010000010 DADOZM010000000
VERSION     DADOZM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 39177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.010"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..562
                     /locus_tag="J8I47_000441"
     CDS             <1..562
                     /locus_tag="J8I47_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ6321906.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="J8I47_000442"
     CDS             762..1076
                     /locus_tag="J8I47_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6321907.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="J8I47_000443"
     CDS             1097..1474
                     /locus_tag="J8I47_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6321908.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="J8I47_000444"
     CDS             1484..2257
                     /locus_tag="J8I47_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321909.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="J8I47_000445"
     CDS             2279..3682
                     /locus_tag="J8I47_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ6321910.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="J8I47_000446"
     CDS             3864..5048
                     /locus_tag="J8I47_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ6321911.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="J8I47_000447"
     CDS             5045..5335
                     /locus_tag="J8I47_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321912.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="J8I47_000448"
     CDS             5332..5553
                     /locus_tag="J8I47_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321913.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="J8I47_000449"
     CDS             5595..6374
                     /locus_tag="J8I47_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAZ6321914.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="J8I47_000450"
     CDS             6441..6935
                     /locus_tag="J8I47_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ6321915.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="J8I47_000451"
     CDS             6991..7629
                     /locus_tag="J8I47_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321916.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="J8I47_000452"
     CDS             7701..8096
                     /locus_tag="J8I47_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6321917.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="J8I47_000453"
     CDS             8080..10533
                     /locus_tag="J8I47_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6321918.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="J8I47_000454"
     CDS             10530..12557
                     /locus_tag="J8I47_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321919.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="J8I47_000455"
     CDS             12554..13576
                     /locus_tag="J8I47_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ6321920.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="J8I47_000456"
     CDS             13593..14522
                     /locus_tag="J8I47_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6321921.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="J8I47_000457"
     CDS             14767..14883
                     /locus_tag="J8I47_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAZ6321922.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="J8I47_000458"
     CDS             14899..15702
                     /locus_tag="J8I47_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ6321923.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15877..16173
                     /locus_tag="J8I47_000459"
     CDS             15877..16173
                     /locus_tag="J8I47_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321924.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="J8I47_000460"
     CDS             16180..18132
                     /locus_tag="J8I47_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAZ6321925.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="J8I47_000461"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="J8I47_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAZ6321926.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="J8I47_000462"
     CDS             19063..20103
                     /locus_tag="J8I47_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAZ6321927.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="J8I47_000463"
     CDS             20201..20389
                     /locus_tag="J8I47_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6321928.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="J8I47_000464"
     CDS             complement(20447..20800)
                     /locus_tag="J8I47_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6321929.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="J8I47_000465"
     CDS             21331..21753
                     /locus_tag="J8I47_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ6321930.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="J8I47_000466"
     CDS             21750..21980
                     /locus_tag="J8I47_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6321931.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="J8I47_000467"
     CDS             22478..22678
                     /locus_tag="J8I47_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ6321932.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="J8I47_000468"
     CDS             22705..23898
                     /locus_tag="J8I47_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6321933.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="J8I47_000469"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="J8I47_000469"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAZ6321934.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="J8I47_000470"
     CDS             complement(25590..26513)
                     /locus_tag="J8I47_000470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAZ6321935.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="J8I47_000471"
     CDS             26709..27314
                     /locus_tag="J8I47_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6321936.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="J8I47_000472"
     CDS             27622..28638
                     /locus_tag="J8I47_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6321937.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="J8I47_000473"
     CDS             28779..30080
                     /locus_tag="J8I47_000473"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAZ6321938.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="J8I47_000474"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="J8I47_000474"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAZ6321939.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="J8I47_000475"
     CDS             30809..31207
                     /locus_tag="J8I47_000475"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAZ6321940.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="J8I47_000476"
     CDS             31273..32355
                     /locus_tag="J8I47_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6321941.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="J8I47_000477"
     CDS             32567..34129
                     /locus_tag="J8I47_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6321942.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="J8I47_000478"
     CDS             34122..35255
                     /locus_tag="J8I47_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321943.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="J8I47_000479"
     CDS             35255..36211
                     /locus_tag="J8I47_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6321944.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="J8I47_000480"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="J8I47_000480"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAZ6321945.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="J8I47_000481"
     CDS             37500..38318
                     /locus_tag="J8I47_000481"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ6321946.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="J8I47_000482"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="J8I47_000482"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ6321947.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        12979 a         6763 c         8013 g        11422 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaa
//
LOCUS       DADOZM010000011        35651 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.011, whole genome shotgun sequence.
ACCESSION   DADOZM010000011 DADOZM010000000
VERSION     DADOZM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.011"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            331..2097
                     /locus_tag="J8I47_000483"
     CDS             331..2097
                     /locus_tag="J8I47_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAZ6321948.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2199..3458
                     /locus_tag="J8I47_000484"
     CDS             2199..3458
                     /locus_tag="J8I47_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6321949.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3605..4906
                     /locus_tag="J8I47_000485"
     CDS             3605..4906
                     /locus_tag="J8I47_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6321950.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4909..5760
                     /locus_tag="J8I47_000486"
     CDS             4909..5760
                     /locus_tag="J8I47_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6321951.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5773..6588
                     /locus_tag="J8I47_000487"
     CDS             5773..6588
                     /locus_tag="J8I47_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAZ6321952.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6739..7905
                     /locus_tag="J8I47_000488"
     CDS             6739..7905
                     /locus_tag="J8I47_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6321953.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8135..8782
                     /locus_tag="J8I47_000489"
     CDS             8135..8782
                     /locus_tag="J8I47_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAZ6321954.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8866..9810
                     /locus_tag="J8I47_000490"
     CDS             8866..9810
                     /locus_tag="J8I47_000490"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAZ6321955.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9945..11396
                     /locus_tag="J8I47_000491"
     CDS             9945..11396
                     /locus_tag="J8I47_000491"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAZ6321956.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11417..12253
                     /locus_tag="J8I47_000492"
     CDS             11417..12253
                     /locus_tag="J8I47_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAZ6321957.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12253..12912
                     /locus_tag="J8I47_000493"
     CDS             12253..12912
                     /locus_tag="J8I47_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ6321958.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13038..13727
                     /locus_tag="J8I47_000494"
     CDS             13038..13727
                     /locus_tag="J8I47_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321959.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13733..14689
                     /locus_tag="J8I47_000495"
     CDS             13733..14689
                     /locus_tag="J8I47_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAZ6321960.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14733..15245
                     /locus_tag="J8I47_000496"
     CDS             14733..15245
                     /locus_tag="J8I47_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6321961.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAAFVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15501..16049)
                     /locus_tag="J8I47_000497"
     CDS             complement(15501..16049)
                     /locus_tag="J8I47_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6321962.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16138..17319)
                     /locus_tag="J8I47_000498"
     CDS             complement(16138..17319)
                     /locus_tag="J8I47_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAZ6321963.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17555..19063)
                     /locus_tag="J8I47_000499"
     CDS             complement(17555..19063)
                     /locus_tag="J8I47_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAZ6321964.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19222..19413
                     /locus_tag="J8I47_000500"
     CDS             19222..19413
                     /locus_tag="J8I47_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321965.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19616..20533
                     /locus_tag="J8I47_000501"
     CDS             19616..20533
                     /locus_tag="J8I47_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAZ6321966.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20526..21599
                     /locus_tag="J8I47_000502"
     CDS             20526..21599
                     /locus_tag="J8I47_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321967.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21747..22265
                     /locus_tag="J8I47_000503"
     CDS             21747..22265
                     /locus_tag="J8I47_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321968.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22246..22638
                     /locus_tag="J8I47_000504"
     CDS             22246..22638
                     /locus_tag="J8I47_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAZ6321969.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22714..23649
                     /locus_tag="J8I47_000505"
     CDS             22714..23649
                     /locus_tag="J8I47_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAZ6321970.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23782..24561
                     /locus_tag="J8I47_000506"
     CDS             23782..24561
                     /locus_tag="J8I47_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAZ6321971.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24554..24871
                     /locus_tag="J8I47_000507"
     CDS             24554..24871
                     /locus_tag="J8I47_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAZ6321972.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25115..26725
                     /locus_tag="J8I47_000508"
     CDS             25115..26725
                     /locus_tag="J8I47_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ6321973.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26882..27730
                     /locus_tag="J8I47_000509"
     CDS             26882..27730
                     /locus_tag="J8I47_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ6321974.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27961..28440
                     /locus_tag="J8I47_000510"
     CDS             27961..28440
                     /locus_tag="J8I47_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321975.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28473..30701
                     /locus_tag="J8I47_000511"
     CDS             28473..30701
                     /locus_tag="J8I47_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAZ6321976.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30830..32473
                     /locus_tag="J8I47_000512"
     CDS             30830..32473
                     /locus_tag="J8I47_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAZ6321977.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32546..33091)
                     /locus_tag="J8I47_000513"
     CDS             complement(32546..33091)
                     /locus_tag="J8I47_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAZ6321978.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33114..33599)
                     /locus_tag="J8I47_000514"
     CDS             complement(33114..33599)
                     /locus_tag="J8I47_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ6321979.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33589..34401)
                     /locus_tag="J8I47_000515"
     CDS             complement(33589..34401)
                     /locus_tag="J8I47_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAZ6321980.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34540..>35651)
                     /locus_tag="J8I47_000516"
     CDS             complement(34540..>35651)
                     /locus_tag="J8I47_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAZ6321981.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11670 a         6410 c         7118 g        10453 t
ORIGIN      
        1 cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat gtttcattct
       61 tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga aaattggaga
      121 aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga ctaaaacttt
      181 taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa
      241 ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg
      301 acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg
      361 gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg
      421 aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa
      481 aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat
      541 tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg
      601 gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg
      661 attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact
      721 attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac
      781 ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga
      841 gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac
      901 cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac
      961 gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac
     1021 ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat
     1081 atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat
     1141 aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca
     1201 gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta
     1261 aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa
     1321 gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg
     1381 aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt
     1441 tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca
     1501 tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc
     1561 agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac
     1621 ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg
     1681 atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg
     1741 acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag
     1801 aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa
     1861 cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa
     1921 gataagctct atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag
     1981 tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag
     2041 ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa
     2101 ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag
     2161 ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac
     2221 agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg
     2281 gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt
     2341 ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga
     2401 aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc
     2461 ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac
     2521 agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca
     2581 aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg
     2641 ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga
     2701 atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt
     2761 tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg
     2821 tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag
     2881 aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca
     2941 taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca
     3001 ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca
     3061 aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg
     3121 tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga
     3181 atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag
     3241 ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg
     3301 aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa
     3361 acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag
     3421 taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt
     3481 ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc
     3541 ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat
     3601 tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact
     3661 tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc
     3721 catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt
     3781 ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac
     3841 tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc
     3901 ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt
     3961 catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct
     4021 catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta
     4081 tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt
     4141 tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt
     4201 gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt
     4261 tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg
     4321 ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg
     4381 tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc
     4441 aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg
     4501 gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga
     4561 cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac
     4621 gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt
     4681 caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg
     4741 agctggttct actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc
     4801 gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc
     4861 attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt
     4921 cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta
     4981 atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca
     5041 ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact
     5101 cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc
     5161 ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat
     5221 cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact
     5281 atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac
     5341 tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa
     5401 ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg
     5461 catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa
     5521 gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga
     5581 acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac
     5641 caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta
     5701 ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag
     5761 gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact
     5821 caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt
     5881 ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt
     5941 ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt
     6001 tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc
     6061 cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt
     6121 attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc
     6181 tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc
     6241 caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat
     6301 agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc
     6361 tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt
     6421 gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt
     6481 atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt
     6541 ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg
     6601 atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg
     6661 aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt
     6721 tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct
     6781 ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg
     6841 gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca
     6901 agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga
     6961 agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa
     7021 gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat
     7081 atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg
     7141 acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat
     7201 aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc
     7261 ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact
     7321 gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat
     7381 ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt
     7441 gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat
     7501 ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc
     7561 cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat
     7621 gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat
     7681 ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat
     7741 caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta
     7801 tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg
     7861 cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac
     7921 tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt
     7981 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
     8041 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
     8101 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
     8161 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
     8221 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
     8281 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
     8341 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
     8401 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
     8461 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
     8521 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
     8581 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
     8641 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
     8701 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
     8761 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
     8821 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
     8881 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
     8941 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
     9001 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
     9061 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
     9121 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
     9181 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
     9241 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
     9301 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
     9361 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
     9421 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
     9481 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
     9541 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
     9601 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
     9661 ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct
     9721 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
     9781 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
     9841 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
     9901 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
     9961 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
    10021 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
    10081 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
    10141 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
    10201 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
    10261 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
    10321 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
    10381 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
    10441 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
    10501 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
    10561 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
    10621 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
    10681 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
    10741 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
    10801 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
    10861 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
    10921 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
    10981 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
    11041 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
    11101 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
    11161 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
    11221 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
    11281 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
    11341 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
    11401 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
    11461 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
    11521 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
    11581 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
    11641 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
    11701 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
    11761 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
    11821 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
    11881 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
    11941 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
    12001 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
    12061 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
    12121 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
    12181 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
    12241 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
    12301 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
    12361 aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta
    12421 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
    12481 ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
    12541 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    12601 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    12661 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    12721 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    12781 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    12841 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
    12901 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    12961 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    13021 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    13081 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    13141 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    13201 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    13261 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    13321 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    13381 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    13441 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    13501 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    13561 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    13621 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    13681 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    13741 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    13801 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    13861 aaatgtgatc gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca
    13921 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    13981 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    14041 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    14101 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    14161 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    14221 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    14281 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    14341 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    14401 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    14461 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    14521 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    14581 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    14641 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    14701 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    14761 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    14821 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    14881 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcatttgtct
    14941 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    15001 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    15061 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    15121 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    15181 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    15241 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    15301 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    15361 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    15421 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
    15481 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
    15541 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
    15601 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
    15661 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
    15721 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
    15781 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
    15841 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
    15901 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
    15961 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
    16021 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
    16081 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
    16141 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
    16201 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
    16261 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
    16321 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
    16381 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
    16441 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
    16501 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
    16561 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
    16621 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
    16681 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
    16741 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
    16801 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
    16861 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
    16921 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
    16981 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
    17041 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
    17101 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
    17161 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
    17221 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
    17281 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
    17341 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
    17401 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
    17461 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
    17521 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
    17581 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
    17641 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
    17701 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
    17761 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
    17821 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
    17881 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
    17941 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    18001 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    18061 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    18121 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    18181 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    18241 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    18301 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    18361 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    18421 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    18481 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
    18541 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    18601 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    18661 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    18721 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    18781 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    18841 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    18901 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
    18961 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    19021 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    19081 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    19141 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    19201 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    19261 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    19321 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    19381 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    19441 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    19501 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
    19561 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
    19621 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca
    19681 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
    19741 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
    19801 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
    19861 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
    19921 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
    19981 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
    20041 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
    20101 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
    20161 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
    20221 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
    20281 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
    20341 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
    20401 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
    20461 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
    20521 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
    20581 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
    20641 aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata
    20701 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
    20761 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
    20821 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
    20881 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
    20941 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
    21001 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
    21061 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
    21121 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
    21181 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
    21241 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
    21301 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
    21361 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
    21421 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
    21481 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
    21541 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
    21601 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
    21661 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
    21721 ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat
    21781 tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa
    21841 tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca
    21901 tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc
    21961 aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag
    22021 tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa
    22081 taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc
    22141 ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt
    22201 ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt
    22261 tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat
    22321 acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg
    22381 ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat
    22441 ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg
    22501 cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg
    22561 aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc
    22621 aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc
    22681 agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg
    22741 ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc
    22801 tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc
    22861 agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg
    22921 catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt
    22981 ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc
    23041 catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa
    23101 ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc
    23161 acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac
    23221 ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc
    23281 gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc
    23341 gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc
    23401 gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata
    23461 aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt
    23521 aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct
    23581 cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct
    23641 cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt
    23701 taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt
    23761 gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg
    23821 catgcagtga tgccactttg tataagctat ataccagtag gattagcttg cggagtattg
    23881 ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc
    23941 ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca
    24001 ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt
    24061 tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa
    24121 aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct
    24181 ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt
    24241 ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc
    24301 attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca
    24361 ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca
    24421 acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct
    24481 aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag
    24541 gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt
    24601 ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg
    24661 gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga
    24721 tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat
    24781 tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact
    24841 tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt
    24901 gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat
    24961 gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt
    25021 attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg
    25081 tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac
    25141 attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc
    25201 ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt
    25261 tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat
    25321 tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt
    25381 tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt
    25441 atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc
    25501 tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata
    25561 tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt
    25621 gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat
    25681 attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat
    25741 ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc
    25801 cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact
    25861 accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat
    25921 gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt
    25981 cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac
    26041 agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat
    26101 cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac
    26161 tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag
    26221 tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct
    26281 atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc
    26341 aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat
    26401 gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg
    26461 caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg
    26521 ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt
    26581 gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg
    26641 cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa
    26701 acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac
    26761 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
    26821 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
    26881 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
    26941 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
    27001 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
    27061 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
    27121 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
    27181 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
    27241 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
    27301 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat
    27361 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
    27421 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
    27481 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
    27541 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
    27601 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
    27661 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
    27721 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac
    27781 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
    27841 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
    27901 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
    27961 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
    28021 gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag
    28081 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
    28141 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
    28201 gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
    28261 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
    28321 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
    28381 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
    28441 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
    28501 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
    28561 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
    28621 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
    28681 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
    28741 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
    28801 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
    28861 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
    28921 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
    28981 cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg
    29041 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
    29101 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
    29161 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
    29221 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
    29281 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
    29341 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
    29401 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
    29461 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
    29521 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta
    29581 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
    29641 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
    29701 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
    29761 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
    29821 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
    29881 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
    29941 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
    30001 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
    30061 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
    30121 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
    30181 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
    30241 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
    30301 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
    30361 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
    30421 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
    30481 agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
    30541 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
    30601 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
    30661 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
    30721 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
    30781 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
    30841 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
    30901 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
    30961 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
    31021 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
    31081 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
    31141 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
    31201 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
    31261 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
    31321 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
    31381 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
    31441 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
    31501 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
    31561 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
    31621 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
    31681 aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc
    31741 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
    31801 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
    31861 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
    31921 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
    31981 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
    32041 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
    32101 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
    32161 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
    32221 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
    32281 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
    32341 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
    32401 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
    32461 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
    32521 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
    32581 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
    32641 gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
    32701 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
    32761 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
    32821 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
    32881 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
    32941 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
    33001 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
    33061 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
    33121 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
    33181 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
    33241 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
    33301 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
    33361 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
    33421 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
    33481 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
    33541 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
    33601 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
    33661 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
    33721 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
    33781 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
    33841 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
    33901 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
    33961 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
    34021 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
    34081 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
    34141 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
    34201 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
    34261 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
    34321 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
    34381 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
    34441 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
    34501 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
    34561 ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa
    34621 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
    34681 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
    34741 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
    34801 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
    34861 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
    34921 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
    34981 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
    35041 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
    35101 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
    35161 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
    35221 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
    35281 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
    35341 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
    35401 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
    35461 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
    35521 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
    35581 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
    35641 aagaatagga a
//
LOCUS       DADOZM010000012        35376 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.012, whole genome shotgun sequence.
ACCESSION   DADOZM010000012 DADOZM010000000
VERSION     DADOZM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.012"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(369..995)
                     /locus_tag="J8I47_000517"
     CDS             complement(369..995)
                     /locus_tag="J8I47_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321982.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="J8I47_000518"
     CDS             complement(1290..1625)
                     /locus_tag="J8I47_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAZ6321983.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="J8I47_000519"
     CDS             complement(1612..1896)
                     /locus_tag="J8I47_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAZ6321984.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="J8I47_000520"
     CDS             complement(1952..3475)
                     /locus_tag="J8I47_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ6321985.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="J8I47_000521"
     CDS             complement(3468..4643)
                     /locus_tag="J8I47_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ6321986.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="J8I47_000522"
     CDS             complement(4647..4832)
                     /locus_tag="J8I47_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321987.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="J8I47_000523"
     CDS             complement(4798..6492)
                     /locus_tag="J8I47_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ6321988.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="J8I47_000524"
     CDS             complement(6489..6962)
                     /locus_tag="J8I47_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAZ6321989.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="J8I47_000525"
     CDS             complement(7031..7186)
                     /locus_tag="J8I47_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAZ6321990.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="J8I47_000526"
     CDS             complement(7320..7700)
                     /locus_tag="J8I47_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ6321991.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="J8I47_000527"
     CDS             complement(7704..7919)
                     /locus_tag="J8I47_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321992.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="J8I47_000528"
     CDS             complement(7922..8329)
                     /locus_tag="J8I47_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAZ6321993.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="J8I47_000529"
     CDS             complement(8595..10070)
                     /locus_tag="J8I47_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAZ6321994.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="J8I47_000530"
     CDS             complement(10060..10908)
                     /locus_tag="J8I47_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAZ6321995.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="J8I47_000531"
     CDS             complement(10945..11307)
                     /locus_tag="J8I47_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321996.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="J8I47_000532"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="J8I47_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="J8I47_000533"
     CDS             complement(12228..12539)
                     /locus_tag="J8I47_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321997.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="J8I47_000534"
     CDS             complement(12583..12876)
                     /locus_tag="J8I47_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6321998.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="J8I47_000535"
     CDS             13070..13717
                     /locus_tag="J8I47_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6321999.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="J8I47_000536"
     CDS             13778..14923
                     /locus_tag="J8I47_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6322000.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="J8I47_000537"
     CDS             complement(15004..15309)
                     /locus_tag="J8I47_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAZ6322001.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="J8I47_000538"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="J8I47_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAZ6322002.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="J8I47_000539"
     CDS             complement(15762..16028)
                     /locus_tag="J8I47_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAZ6322003.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="J8I47_000540"
     CDS             complement(16283..17008)
                     /locus_tag="J8I47_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAZ6322004.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="J8I47_000541"
     tRNA            complement(17369..17439)
                     /locus_tag="J8I47_000541"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="J8I47_000542"
     CDS             17556..18308
                     /locus_tag="J8I47_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAZ6322005.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="J8I47_000543"
     CDS             18324..18803
                     /locus_tag="J8I47_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAZ6322006.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="J8I47_000544"
     CDS             complement(19054..20634)
                     /locus_tag="J8I47_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAZ6322007.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="J8I47_000545"
     CDS             complement(20627..21370)
                     /locus_tag="J8I47_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322008.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..22322)
                     /locus_tag="J8I47_000546"
     CDS             complement(21546..22322)
                     /locus_tag="J8I47_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322009.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22570..23406)
                     /locus_tag="J8I47_000547"
     CDS             complement(22570..23406)
                     /locus_tag="J8I47_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAZ6322010.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23417..24346)
                     /locus_tag="J8I47_000548"
     CDS             complement(23417..24346)
                     /locus_tag="J8I47_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAZ6322011.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24538..24891
                     /locus_tag="J8I47_000549"
     CDS             24538..24891
                     /locus_tag="J8I47_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ6322012.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24965..25621)
                     /locus_tag="J8I47_000550"
     CDS             complement(24965..25621)
                     /locus_tag="J8I47_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAZ6322013.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25847..27781
                     /locus_tag="J8I47_000551"
     CDS             25847..27781
                     /locus_tag="J8I47_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ6322014.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27867..29090)
                     /locus_tag="J8I47_000552"
     CDS             complement(27867..29090)
                     /locus_tag="J8I47_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAZ6322015.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29084..30982)
                     /locus_tag="J8I47_000553"
     CDS             complement(29084..30982)
                     /locus_tag="J8I47_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAZ6322016.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30976..32094)
                     /locus_tag="J8I47_000554"
     CDS             complement(30976..32094)
                     /locus_tag="J8I47_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAZ6322017.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32177..33010)
                     /locus_tag="J8I47_000555"
     CDS             complement(32177..33010)
                     /locus_tag="J8I47_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322018.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33003..33920)
                     /locus_tag="J8I47_000556"
     CDS             complement(33003..33920)
                     /locus_tag="J8I47_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322019.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34147..35271)
                     /locus_tag="J8I47_000557"
     CDS             complement(34147..35271)
                     /locus_tag="J8I47_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6322020.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10619 a         7132 c         5781 g        11844 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata
    22021 acagttattg ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt
    22081 tctctaagca tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg
    22141 aaaaaaatat ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg
    22201 ttcaatccct gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa
    22261 atttctacta gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc
    22321 ataaaaagta cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca
    22381 tacatgttag gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga
    22441 tatcaaatac tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg
    22501 caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat
    22561 tttgttcttt taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc
    22621 gtttgtaaca actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat
    22681 ggttccttct attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt
    22741 caacaaacca aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa
    22801 gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt
    22861 gccaatgaat tcgctagggt aattataaat tagctccata acttctaaat agttttcatc
    22921 tgtgatcttg atagtttgct tatccccata cttttctttc aactcattca tttggtctaa
    22981 gtagtctgat ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc
    23041 cggatcccct actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt
    23101 agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac
    23161 ataagcgatt ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc
    23221 cttttcttct gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc
    23281 agataaaatc attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag
    23341 tttgccagaa acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg
    23401 cagcatattt tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc
    23461 cgatcagcga taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga
    23521 tatcgaccat tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta
    23581 acaaagaaga tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata
    23641 acagcatgat gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg
    23701 gtaaataagt ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat
    23761 caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt
    23821 ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta
    23881 ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac
    23941 caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat
    24001 tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca
    24061 taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta
    24121 cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga
    24181 agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg
    24241 cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga
    24301 aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt
    24361 ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc
    24421 tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta
    24481 ttcccattaa attaaggtct catacatatc acttctatca taaaagggag aataaaaatg
    24541 aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc
    24601 atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa
    24661 cttctcacac attatgatat tccggcactt catttggatg catttgaaac accagaagta
    24721 gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa
    24781 gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg
    24841 acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc
    24901 aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt
    24961 atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca
    25021 aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca
    25081 atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta
    25141 cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga
    25201 tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga
    25261 tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt
    25321 attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat
    25381 cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac
    25441 gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa
    25501 cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta
    25561 aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca
    25621 cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt
    25681 ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat
    25741 ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa
    25801 tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca
    25861 agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga
    25921 aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt
    25981 attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac
    26041 agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa
    26101 cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga
    26161 gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata
    26221 cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg
    26281 ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt
    26341 atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat
    26401 tttgattcta gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa
    26461 ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga
    26521 taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa
    26581 ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga
    26641 aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc
    26701 ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga
    26761 tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg
    26821 aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga
    26881 accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat
    26941 cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga
    27001 aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac
    27061 aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat
    27121 ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct
    27181 gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa
    27241 ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga
    27301 aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat
    27361 caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg
    27421 agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc
    27481 acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa
    27541 agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat
    27601 ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct
    27661 ggaagatcac gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca
    27721 agaaacaaaa ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta
    27781 acgaaaaaac caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc
    27841 aaacaaaaat tccatattta cctttttcac caataagaag accaattact agtcattctc
    27901 actaaagctt ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt
    27961 ttgggcttat tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat
    28021 ctttctttca attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt
    28081 ttgtctaata gacctgattc tattaattct aataatccac aaaccatgat caccatacta
    28141 gatgtatctt tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta
    28201 tcttctggca accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt
    28261 tgttctttca aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta
    28321 gcccaacagg aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct
    28381 ttgacatcca tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct
    28441 gcattttgaa tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc
    28501 gcccaataca ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa
    28561 ggatcactag gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt
    28621 aatgtttctg cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa
    28681 tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga
    28741 cagtcaatcc tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga
    28801 tcacccgtct tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca
    28861 atattgtctc ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga
    28921 ctgttactga tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta
    28981 ttccattctt tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt
    29041 atctcttcaa acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc
    29101 tttttttatt gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg
    29161 gacgattgtt ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc
    29221 cgagccaatg ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc
    29281 atgtcatcaa gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat
    29341 cgtcacttaa acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt
    29401 cttcttctat gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat
    29461 atagaacata ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag
    29521 gaataagcca agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct
    29581 ctttcttaca tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga
    29641 tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg
    29701 aatccattgc catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac
    29761 tagaataagc aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt
    29821 gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg
    29881 aaagaagttt agttccttca cgaataggca taggcatttc tttagccgtc caatactcat
    29941 ggtcttcagg tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac
    30001 tattataggg ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt
    30061 tttcatcaaa tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga
    30121 tttcacctaa tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa
    30181 ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa
    30241 aatgaatata gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct
    30301 tttctggttc tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat
    30361 aatcttggcg acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta
    30421 tattatcaaa tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa
    30481 ttgttccgac aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa
    30541 tttgcgagaa ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata
    30601 attgttcatg atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc
    30661 tttcatgtat ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg
    30721 atagaattaa tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata
    30781 aaacccgaga gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta
    30841 tataatcaat ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt
    30901 taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat
    30961 tttctcctct ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc
    31021 aataaaaaaa taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc
    31081 ttctgctccg cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc
    31141 tgtcttttgg agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac
    31201 atatcctgct aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt
    31261 cgaatctttt acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat
    31321 aaaatttact gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata
    31381 acttaacgcg aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt
    31441 gtatccttgc ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat
    31501 atgcccggtt gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg
    31561 agcaatttta gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa
    31621 attcatcata ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata
    31681 gttccctgaa atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg
    31741 actatgtttg ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc
    31801 tacatcatga tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg
    31861 tattgctgcc tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc
    31921 actccaaaaa ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt
    31981 tccgttgtaa gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt
    32041 ttcagaagct acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat
    32101 cccgccattc ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta
    32161 acattcgtta ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact
    32221 gcgcccacac ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg
    32281 cgccataatt aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa
    32341 ccgtatattt atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc
    32401 ccataaagat aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt
    32461 tcgtaaaaat cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag
    32521 gaaattgact gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga
    32581 tgatcacacc tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa
    32641 tcaatgtggt aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat
    32701 tccccataaa acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta
    32761 aaactgtact gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc
    32821 tcgccatgat ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc
    32881 cacttaaaat ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca
    32941 aaaatattaa ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt
    33001 gtttagtcat ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca
    33061 aacaccatcg tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt
    33121 ttatataaat tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag
    33181 aatatatttg ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt
    33241 aaaatgattg gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct
    33301 ccatctattt cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat
    33361 aaaatagtgt tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc
    33421 caaaaaggat ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg
    33481 aataacccag tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta
    33541 atggttggaa tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc
    33601 aataaaacag caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata
    33661 atcaaaacat tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg
    33721 tcaaatccaa caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata
    33781 atgatgggaa tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa
    33841 gcactttttt tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt
    33901 ggtaacgaag attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt
    33961 tacgccgtac ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc
    34021 tgtttgccaa tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc
    34081 tggtaaaaca gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg
    34141 tcctccttag tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga
    34201 tgtagccacg cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt
    34261 aagaaattgt tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc
    34321 tcctgatact gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg
    34381 ccatttgtca gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc
    34441 aacaaacatt gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac
    34501 aatatctgag tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt
    34561 cagacggata tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat
    34621 aaacaattct tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt
    34681 taatgctttt gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc
    34741 tatttcagca aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca
    34801 aggaataatc gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa
    34861 ctgatcgcca tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa
    34921 tttttccagc acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc
    34981 gtgtgttacc aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac
    35041 aatttttaag tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac
    35101 atagtttgtc agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat
    35161 tggatctgcg atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa
    35221 taatagggta atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc
    35281 ttctttcttt tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt
    35341 aacacaaata tttatataaa aaaatatttt acttgt
//
LOCUS       DADOZM010000013        34773 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.013, whole genome shotgun sequence.
ACCESSION   DADOZM010000013 DADOZM010000000
VERSION     DADOZM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.013"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            65..304
                     /locus_tag="J8I47_000558"
     CDS             65..304
                     /locus_tag="J8I47_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322021.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            371..610
                     /locus_tag="J8I47_000559"
     CDS             371..610
                     /locus_tag="J8I47_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322022.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            696..1367
                     /locus_tag="J8I47_000560"
     CDS             696..1367
                     /locus_tag="J8I47_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAZ6322023.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1420..3396
                     /locus_tag="J8I47_000561"
     CDS             1420..3396
                     /locus_tag="J8I47_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ6322024.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3409..4065
                     /locus_tag="J8I47_000562"
     CDS             3409..4065
                     /locus_tag="J8I47_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ6322025.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            4161..4988
                     /locus_tag="J8I47_000563"
     CDS             4161..4988
                     /locus_tag="J8I47_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322026.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            4998..5246
                     /locus_tag="J8I47_000564"
     CDS             4998..5246
                     /locus_tag="J8I47_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322027.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            5277..7388
                     /locus_tag="J8I47_000565"
     CDS             5277..7388
                     /locus_tag="J8I47_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ6322028.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7426..9483)
                     /locus_tag="J8I47_000566"
     CDS             complement(7426..9483)
                     /locus_tag="J8I47_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAZ6322029.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9597..9809)
                     /locus_tag="J8I47_000567"
     CDS             complement(9597..9809)
                     /locus_tag="J8I47_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322030.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9996..10463
                     /locus_tag="J8I47_000568"
     CDS             9996..10463
                     /locus_tag="J8I47_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322031.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            10460..10702
                     /locus_tag="J8I47_000569"
     CDS             10460..10702
                     /locus_tag="J8I47_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322032.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            10720..11022
                     /locus_tag="J8I47_000570"
     CDS             10720..11022
                     /locus_tag="J8I47_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322033.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11093..13147
                     /locus_tag="J8I47_000571"
     CDS             11093..13147
                     /locus_tag="J8I47_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322034.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13147..14979
                     /locus_tag="J8I47_000572"
     CDS             13147..14979
                     /locus_tag="J8I47_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAZ6322035.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            15674..17590
                     /gene="ltrA"
                     /locus_tag="J8I47_000573"
     CDS             15674..17590
                     /gene="ltrA"
                     /locus_tag="J8I47_000573"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ6322036.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            17707..18507
                     /locus_tag="J8I47_000574"
                     /pseudo
     CDS             17707..18507
                     /locus_tag="J8I47_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            18504..21035
                     /locus_tag="J8I47_000575"
     CDS             18504..21035
                     /locus_tag="J8I47_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322037.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            21085..21417
                     /locus_tag="J8I47_000576"
     CDS             21085..21417
                     /locus_tag="J8I47_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322038.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21418..22011
                     /locus_tag="J8I47_000577"
     CDS             21418..22011
                     /locus_tag="J8I47_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322039.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22174..23868
                     /locus_tag="J8I47_000578"
     CDS             22174..23868
                     /locus_tag="J8I47_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAZ6322040.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            24556..26433
                     /gene="ltrA"
                     /locus_tag="J8I47_000579"
     CDS             24556..26433
                     /gene="ltrA"
                     /locus_tag="J8I47_000579"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ6322041.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            26564..26941
                     /locus_tag="J8I47_000580"
                     /pseudo
     CDS             26564..26941
                     /locus_tag="J8I47_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            26941..28146
                     /locus_tag="J8I47_000581"
     CDS             26941..28146
                     /locus_tag="J8I47_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ6322042.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            28162..28806
                     /locus_tag="J8I47_000582"
     CDS             28162..28806
                     /locus_tag="J8I47_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322043.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            28817..29623
                     /locus_tag="J8I47_000583"
     CDS             28817..29623
                     /locus_tag="J8I47_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322044.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            29645..29875
                     /locus_tag="J8I47_000584"
     CDS             29645..29875
                     /locus_tag="J8I47_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322045.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            30421..30696
                     /locus_tag="J8I47_000585"
     CDS             30421..30696
                     /locus_tag="J8I47_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322046.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            30738..31160
                     /locus_tag="J8I47_000586"
     CDS             30738..31160
                     /locus_tag="J8I47_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322047.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            31179..32771
                     /locus_tag="J8I47_000587"
     CDS             31179..32771
                     /locus_tag="J8I47_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322048.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            32783..33118
                     /locus_tag="J8I47_000588"
     CDS             32783..33118
                     /locus_tag="J8I47_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322049.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            33265..33459
                     /locus_tag="J8I47_000589"
     CDS             33265..33459
                     /locus_tag="J8I47_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322050.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            33491..>34773
                     /locus_tag="J8I47_000590"
     CDS             33491..>34773
                     /locus_tag="J8I47_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322051.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKG"
BASE COUNT        12860 a         5444 c         7081 g         9388 t
ORIGIN      
        1 gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt aggaggaaaa
       61 ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa aaggaaggta
      121 tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc tgtctaaagg
      181 aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa cccataatgg
      241 aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga
      301 ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caaggaaggt
      361 aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga
      421 agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg tcatgcaata
      481 tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa
      541 ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac tattgttaga
      601 agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg aaatgagaaa
      661 aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta tttggttttt
      721 taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac caaaaacatg
      781 aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa atcaatgaac
      841 atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct ttggggcaaa
      901 tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc tttgcacaaa
      961 ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat caatacactt
     1021 tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa ggaaactatg
     1081 tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt caaaagttgt
     1141 cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac aaggtaacgc
     1201 gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa gagaactctt
     1261 tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact tgtgtttata
     1321 cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa ggttttaatc
     1381 aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta
     1441 aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt tcaggggtat
     1501 caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa tatcacggtt
     1561 cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg gatcaaaaac
     1621 gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa ggcagattat
     1681 ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag agaagattct
     1741 gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa gggatctaac
     1801 agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga aaatggtgag
     1861 atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata tgtggtttat
     1921 gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt tgtgattgcg
     1981 ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta tcctaagaac
     2041 attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc agcccaaagc
     2101 aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc tggaaaagga
     2161 acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac tgatttgact
     2221 ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc agatcaagaa
     2281 ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca taataaatta
     2341 acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta cgtgaactat
     2401 aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca ttctttccaa
     2461 attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat tgcaggtgga
     2521 acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag cgtatttaag
     2581 tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa tatcaaagta
     2641 acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat ccaaaattca
     2701 gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga tactgtagca
     2761 agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga cagcgcagct
     2821 gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc atttaatcag
     2881 gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa attcttaaaa
     2941 gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg cgcagaattt
     3001 gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga taaagacggt
     3061 gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa atcagataaa
     3121 gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga agaaacaaaa
     3181 gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa caaaaactca
     3241 tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat tccattaaca
     3301 ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg agcaggggca
     3361 ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat gattattcgt
     3421 aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt gctgtcggca
     3481 gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca acaacagcaa
     3541 agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt tgcgaaaata
     3601 gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa actaccaatt
     3661 tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga aggaactggc
     3721 gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg actttataaa
     3781 gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt tattaaggta
     3841 aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc agaagaatta
     3901 caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat cacgttaatg
     3961 acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga acgagtgtca
     4021 ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat caatttcatt
     4081 ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt tatttataaa
     4141 aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt gtagcagctc
     4201 tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact actccaacta
     4261 ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt tcgaagaaca
     4321 ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa tcaacgtcag
     4381 aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa gtaatcaagc
     4441 aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa ggaaggcaag
     4501 tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc acgaatatct
     4561 tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata attgagtcaa
     4621 aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg actgaaggag
     4681 aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa gtacctgcaa
     4741 acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt tcaaatagcg
     4801 aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg acggatgttt
     4861 ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa gttgtatcaa
     4921 ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat ttatttttta
     4981 acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta tcttttttag
     5041 ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac ccaagatatc
     5101 aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta gaagtgatta
     5161 acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca ggattggtaa
     5221 cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa taatagatgg
     5281 agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact ttagtgttgt
     5341 ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5401 cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat tcattgagaa
     5461 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5521 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa attgttgccc
     5581 attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta actttttcta
     5641 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatgggaaaa
     5701 agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc atgcagtcaa
     5761 tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca aaagtagctt
     5821 tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca gctggtattg
     5881 agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag caacgtgtgt
     5941 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     6001 ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca gcatgtgcag
     6061 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6121 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6181 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6241 atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca caaccatcga
     6301 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6361 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6421 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6481 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6541 cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca ggtaatacag
     6601 tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt agggactaca
     6661 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6721 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa gtagaaaact
     6781 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6841 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6901 tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat tacaagcaat
     6961 ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct gcgcgagtga
     7021 tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat gatagccatt
     7081 caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt ttagatggac
     7141 gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7201 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7261 taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7321 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7381 tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt tgaagattgg
     7441 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7501 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7561 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7621 cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc tttccaataa
     7681 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7741 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7801 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7861 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7921 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7981 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
     8041 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     8101 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8161 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8221 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
     8281 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8341 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8401 attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8461 agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8521 aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8581 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8641 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8701 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8761 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8821 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8881 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8941 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     9001 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     9061 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9121 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9181 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9241 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9301 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9361 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9421 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9481 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggttaa
     9541 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9601 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9661 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9721 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9781 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9841 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9901 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9961 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
    10021 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
    10081 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10141 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10201 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10261 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10321 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10381 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca aataaataca
    10441 aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag gaggaatcac
    10501 agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag aacgccgcgg
    10561 tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg acctattacg
    10621 gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa agcttgtttt
    10681 cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc aattacaatg
    10741 aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc attacaaact
    10801 gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc agtggcttcc
    10861 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10921 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10981 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa gtagaaggag
    11041 taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat tcatgttttt
    11101 tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa cgaagaaagt
    11161 gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg aaacggaaga
    11221 acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg ttaaattgac
    11281 tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat acgtttttgg
    11341 ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt taatccgcct
    11401 agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc gaaacgaaaa
    11461 aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga atcaagtgag
    11521 tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa aggatgaatc
    11581 aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc ataaatcaat
    11641 atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg aagaaacaat
    11701 ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg aagacccaac
    11761 atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagagaa
    11821 aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc aatatccgcc
    11881 agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt attcgaaata
    11941 tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg
    12001 taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc
    12061 acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc aagaaattaa
    12121 tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac taaataaaga
    12181 agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg aaaagaaacg
    12241 tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga cgaatcaaga
    12301 agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac gaaaagaaga
    12361 attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa atgaacaact
    12421 gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc agatccaaca
    12481 agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc aacaagaatt
    12541 gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat tagaactaaa
    12601 aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac ggctagaaca
    12661 agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg
    12721 acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc aaacgccagt
    12781 gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat ggatggtggg
    12841 atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat
    12901 caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg aagaagccca
    12961 ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc gtaccaatca
    13021 gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta aggctaaaga
    13081 aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat caaatgaggt
    13141 gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa tttagaaaag
    13201 aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct accagaaaaa
    13261 catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct ccctttagag
    13321 tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa gggaagctta
    13381 caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt tgattcagct
    13441 ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc aatttcgaca
    13501 gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtcctcta
    13561 gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca acagatttat
    13621 ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc aatcacacat
    13681 tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt tggtacctct
    13741 cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc tcgtgcagaa
    13801 aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg tagtaaagat
    13861 gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc actgcaatca
    13921 atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga catagatact
    13981 gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa cgcttcagat
    14041 aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt tgcgttgtta
    14101 gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct cgttgagctt
    14161 ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt caaacagttg
    14221 aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa aaaacaatat
    14281 ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac ggcaatcacg
    14341 ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc aacgattgat
    14401 tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag tcttttaaat
    14461 caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga acgtgtcaaa
    14521 gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc aggtgatcaa
    14581 gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga attttctcaa
    14641 agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg agaaacccat
    14701 ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag taacttatta
    14761 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gcttacacct
    14821 gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt atataaagaa
    14881 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat tcattttatt
    14941 ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag gctagttcca
    15001 acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg ccctgaaggg
    15061 tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt atgaagctcg
    15121 ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc acgcaagtgc
    15181 tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa taaggtgaga
    15241 atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa ctcatagaaa
    15301 tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt aaaaatgtgt
    15361 ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca aaagcaagca
    15421 cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag cacggaggga
    15481 gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta ggtgaataga
    15541 tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga acaggctcaa
    15601 gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa gactaaaaag
    15661 gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa ttagcttaaa
    15721 gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg atgaattgca
    15781 cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta ttgatgacaa
    15841 taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa cggccggata
    15901 cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt taactattat
    15961 taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga tcccgaaacc
    16021 aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag tccagcaatg
    16081 tattctacaa attttagaac caatatgcga agcaaaattt aataagcata gctatggatt
    16141 cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa tcaatcaaag
    16201 taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag taaaccatac
    16261 caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc ttgtaattat
    16321 tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt ttccaaccaa
    16381 gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc tgaatgaatt
    16441 tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga aacaccacta
    16501 tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa gccacacaag
    16561 tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct gccgaacagg
    16621 agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa gattaaagct
    16681 cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa gtgaattctt
    16741 gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca cgtacgtttg
    16801 tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa aaattcaata
    16861 cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga tggtcatcgg
    16921 tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg agatttatag
    16981 aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga aaaatggaat
    17041 ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa tgagatatat
    17101 tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac tcaacaagcg
    17161 gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag agcaacaagc
    17221 tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata agaaatacac
    17281 ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga aatgcgctat
    17341 cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc catatcattt
    17401 gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc atagattaat
    17461 ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac taaatcacta
    17521 tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa tatcaatcaa
    17581 aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg tgtgcggtga
    17641 aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa ctcttaccta
    17701 tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc tggccgaaac
    17761 atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta tggagaacaa
    17821 aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc gacagatgat
    17881 aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca agagaacagt
    17941 accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc agatcgtatc
    18001 ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta
    18061 aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa
    18121 acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat
    18181 gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca aattgattat
    18241 gattatttcc taccagatga atatgctaga aatctctatc atggactgca tgatcaatta
    18301 gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga gaatgagacg
    18361 gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact tcaagaatat
    18421 gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg gttgttattg
    18481 gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc atagccgact
    18541 ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg aaagtcttac
    18601 aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt aatacttttt
    18661 tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa accatttatc
    18721 aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta tttcaaagtt
    18781 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat
    18841 tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta tttatggcca
    18901 tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaagct
    18961 tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct gcttttcaaa
    19021 ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg aataaagtag
    19081 aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa acagcgaata
    19141 tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat aagagtggtt
    19201 tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa ctggatgata
    19261 atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat gtttatactt
    19321 cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa tcttctttgg
    19381 cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag ctggtattta
    19441 aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa cggatgaaaa
    19501 gtgctatcga agatatttct caatccttct tgttattggc atttacaatt ttagaattac
    19561 gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga aagatcaatg
    19621 gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa ggctctcaat
    19681 cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat tctcttctta
    19741 gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa
    19801 gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga tttggccatc
    19861 gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct acaactgtac
    19921 ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag
    19981 ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct gaagatctta
    20041 cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct aaagaattgg
    20101 ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa gcttcagaag
    20161 tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca gtgaaagcta
    20221 atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc aaagatcacc
    20281 aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata gaagaaagaa
    20341 attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa tatcgctata
    20401 aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa gtaaatcaag
    20461 aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa tatcggtata
    20521 gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga aatcaagaag
    20581 tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa gttggaactg
    20641 aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa gatagtcgtt
    20701 tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca ggtactgttc
    20761 aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttat
    20821 ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac
    20881 caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca tttactgcac
    20941 caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg aaagaacaac
    21001 tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta ggccttttct
    21061 ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa ccctccaaga
    21121 tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac gcttggtggc
    21181 tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt ttttgcactc
    21241 ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc ctataataac
    21301 ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga caagaaccat
    21361 tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga tgaataaatg
    21421 cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg aaaaccagaa
    21481 gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg agtcttaatt
    21541 actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt ggaaggatta
    21601 aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg
    21661 ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca aatcgtgcaa
    21721 aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa tctacagcga
    21781 ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg agcagaacag
    21841 gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa ggatttgcaa
    21901 accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc actctacaaa
    21961 aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta ggaatgggga
    22021 agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag aagagaccac
    22081 tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat cttttaacca
    22141 agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa aacagctttt
    22201 tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa tcgggaaata
    22261 gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat gccacaaggt
    22321 ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg atgtgtgaca
    22381 gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat gaacaatcct
    22441 catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc
    22501 aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc gactgaccga
    22561 aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt gacttctaat
    22621 ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac gattcaagaa
    22681 gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta tcgtgcagtg
    22741 acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt cgatgaacaa
    22801 gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat tggcggaggt
    22861 ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg ggaaaccatg
    22921 acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt gtctaccaat
    22981 accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa
    23041 gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga ctttattccc
    23101 tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg gatgatcaat
    23161 cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata tgacgatgga
    23221 tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt agacaaagtg
    23281 gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag
    23341 acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt tacttcctca
    23401 aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc ttatccgacg
    23461 ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc ccaagagaaa
    23521 aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac gtatggcatg
    23581 atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt cttttttgac
    23641 attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta catggctatg
    23701 acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt aattaaagaa
    23761 aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga gtgccataat
    23821 ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa actattgtaa
    23881 taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt ccactgatac
    23941 tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc
    24001 tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct agaggtaaag
    24061 ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta
    24121 ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt
    24181 gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga
    24241 ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg
    24301 catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg
    24361 aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga
    24421 gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa
    24481 agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt
    24541 ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca
    24601 gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat
    24661 aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt
    24721 aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat
    24781 attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat
    24841 aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg
    24901 ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt attagaacca
    24961 atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag atcagcagaa
    25021 cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata tgtggtagat
    25081 gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag acaaatctgg
    25141 tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt aagagcaccc
    25201 gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca aggcggaata
    25261 cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat agcaaatcag
    25321 tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg agcttgggat
    25381 aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat atacctagtg
    25441 agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa aaaagttttc
    25501 tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga agaaaagtca
    25561 aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat gaaattagtg
    25621 accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca aagcatgatt
    25681 aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag caaagatacc
    25741 tttatctata ttggagtcta taacagtatg gttattggga ttcataatta cttcaaaatt
    25801 gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc aagttttgaa
    25861 atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta cgaaggatac
    25921 aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat attaccagta
    25981 gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa atacaccgaa
    26041 gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa gatatcgtgg
    26101 ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga caatcgaatt
    26161 tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct tatcttatct
    26221 gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag ctatgaaaac
    26281 ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt
    26341 gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact caatgagttg
    26401 agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg ggaaaacaga
    26461 atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact
    26521 gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta atccagattt
    26581 tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat
    26641 gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt cttctgataa
    26701 cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta tgcgtatgga
    26761 tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa actcagaatt
    26821 agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct caaaagaaac
    26881 cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata
    26941 atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc aaagcgacgc
    27001 ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat tactgtgttc
    27061 gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc agattcaagt
    27121 agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta taaagaaatc
    27181 aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaaa
    27241 tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc gtttggtacg
    27301 tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga tggttgtctg
    27361 tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta catgaagttt
    27421 cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca atccaatttt
    27481 aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg acgaggtggt
    27541 tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat gtccattatc
    27601 acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg tgaaggtggc
    27661 aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag cagtttagat
    27721 aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga attccgtccg
    27781 aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag tgaaattcac
    27841 gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgca
    27901 tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt tgcgaatagt
    27961 acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt aatcgggata
    28021 cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca agcacaagga
    28081 cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa tagcagcaaa
    28141 aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt aacagctgca
    28201 attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga agaacaagag
    28261 acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat
    28321 tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa
    28381 gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt aactgaagca
    28441 caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagtagaaat
    28501 gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct
    28561 gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt aggtgacgtg
    28621 caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat
    28681 tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa aggtggatta
    28741 actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag ttatttcact
    28801 cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca aaaaacgaaa
    28861 aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc ttgctagtcc
    28921 gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg cctttttaaa
    28981 cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga
    29041 atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc ttaattatcg
    29101 cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag tgatcaaggt
    29161 gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata tcgccatgcg
    29221 tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac cacaagatct
    29281 gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag attatgaaca
    29341 acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag ctcaaaaaca
    29401 aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga agctaaaaga
    29461 tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga ttaatagcta
    29521 ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga tcattcaaaa
    29581 acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca ggtggagtga
    29641 aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga aagtattgat
    29701 taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga atttatctaa
    29761 cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa aattcagatc
    29821 atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca aataaaaaac
    29881 aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct atatcacctt
    29941 aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca aacatcaagc
    30001 acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac aggaagacag
    30061 cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca aaatatttcc
    30121 ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata accttaattt
    30181 ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg taggctgtgc
    30241 ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt gtagatttgt
    30301 gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc aaaatcaaaa
    30361 aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt ggaggaaaat
    30421 atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga caaacgttgt
    30481 gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct aaaagttttg
    30541 atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt tgatcggttg
    30601 ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga atatattgca
    30661 acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg tgaatttata
    30721 aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc
    30781 atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat tatagtgaag
    30841 cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata aatccaattc
    30901 aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg gtttcagagt
    30961 ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca actgtttatc
    31021 aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattctt
    31081 ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca tttgatccaa
    31141 cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg cctgcagtta
    31201 ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac attgattata
    31261 tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa gaacaggaac
    31321 tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg
    31381 aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac
    31441 atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt tcttatatga
    31501 cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa aaagaatacc
    31561 aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa
    31621 atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat ctgaaggaga
    31681 tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttatct
    31741 ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca gataccttag
    31801 atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac
    31861 aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag catatccata
    31921 ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag attgaaaatg
    31981 gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac gcaatgaaaa
    32041 ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg atcggtatgg
    32101 agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca aaagtagtaa
    32161 aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta tataaaaccc
    32221 tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag ctttcaccat
    32281 ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa cctggatatc
    32341 aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt tatggggaaa
    32401 gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag
    32461 gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca ttaatagaaa
    32521 gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc aaacagttgc
    32581 caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa caattccaac
    32641 aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat gcacatgaag
    32701 aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa atggaacaag
    32761 aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag tcatagaagg
    32821 atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt
    32881 tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagttgttga
    32941 gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat attttattat
    33001 tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa atgaagaggg
    33061 aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg aatgttaaat
    33121 aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc aatgaattgt
    33181 tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga aagtgagatt
    33241 ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt ggttgaaaaa
    33301 agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt tgtagtcaca
    33361 gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct gactacgaag
    33421 atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat tttacaagga
    33481 ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg gaaagtccaa
    33541 aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc tatttgatca
    33601 ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt catggtttaa
    33661 ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca agcaacaaga
    33721 aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt ataacaagta
    33781 cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa tcgaacgctt
    33841 tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac gaaaattgga
    33901 aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc ttcctgaagt
    33961 acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa ctatctattt
    34021 tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat ttgaggaatc
    34081 taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat tacttgaaga
    34141 agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc gagtagaatt
    34201 caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg taacactaga
    34261 attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag gctattataa
    34321 attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa tggatctagg
    34381 ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt gcgaacaatc
    34441 acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag tacaaaacga
    34501 gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag aagagattaa
    34561 gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc atactcattc
    34621 acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac gatcctttag
    34681 aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag gtcgtgccgc
    34741 gttgaaaaaa gaaggaatct atatcaaaaa agg
//
LOCUS       DADOZM010000014        33751 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.014, whole genome shotgun sequence.
ACCESSION   DADOZM010000014 DADOZM010000000
VERSION     DADOZM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.014"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            124..2451
                     /locus_tag="J8I47_000591"
     CDS             124..2451
                     /locus_tag="J8I47_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6322052.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2577..2906
                     /locus_tag="J8I47_000592"
     CDS             2577..2906
                     /locus_tag="J8I47_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAZ6322053.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2946..3950
                     /locus_tag="J8I47_000593"
     CDS             2946..3950
                     /locus_tag="J8I47_000593"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ6322054.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3947..4393
                     /locus_tag="J8I47_000594"
     CDS             3947..4393
                     /locus_tag="J8I47_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAZ6322055.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4543..5358
                     /locus_tag="J8I47_000595"
     CDS             4543..5358
                     /locus_tag="J8I47_000595"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ6322056.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5564..6736
                     /locus_tag="J8I47_000596"
     CDS             5564..6736
                     /locus_tag="J8I47_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ6322057.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6898..7392)
                     /locus_tag="J8I47_000597"
     CDS             complement(6898..7392)
                     /locus_tag="J8I47_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322058.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            7975..8397
                     /locus_tag="J8I47_000598"
     CDS             7975..8397
                     /locus_tag="J8I47_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ6322059.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8563..9756)
                     /locus_tag="J8I47_000599"
     CDS             complement(8563..9756)
                     /locus_tag="J8I47_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322060.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9980..10357
                     /locus_tag="J8I47_000600"
     CDS             9980..10357
                     /locus_tag="J8I47_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ6322061.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10345..10683
                     /locus_tag="J8I47_000601"
                     /pseudo
     CDS             10345..10683
                     /locus_tag="J8I47_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10817..11260
                     /locus_tag="J8I47_000602"
     CDS             10817..11260
                     /locus_tag="J8I47_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAZ6322062.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11521..12336
                     /locus_tag="J8I47_000603"
     CDS             11521..12336
                     /locus_tag="J8I47_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322063.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12346..13008
                     /locus_tag="J8I47_000604"
     CDS             12346..13008
                     /locus_tag="J8I47_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ6322064.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13216..14880
                     /gene="alsS"
                     /locus_tag="J8I47_000605"
     CDS             13216..14880
                     /gene="alsS"
                     /locus_tag="J8I47_000605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAZ6322065.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14893..15603
                     /gene="budA"
                     /locus_tag="J8I47_000606"
     CDS             14893..15603
                     /gene="budA"
                     /locus_tag="J8I47_000606"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAZ6322066.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15733..16230
                     /locus_tag="J8I47_000607"
     CDS             15733..16230
                     /locus_tag="J8I47_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAZ6322067.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16415..16675
                     /locus_tag="J8I47_000608"
     CDS             16415..16675
                     /locus_tag="J8I47_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322068.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(17037..17276)
                     /locus_tag="J8I47_000609"
     CDS             complement(17037..17276)
                     /locus_tag="J8I47_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322069.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17562..17876
                     /locus_tag="J8I47_000610"
     CDS             17562..17876
                     /locus_tag="J8I47_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322070.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(18310..18676)
                     /gene="ssrA"
                     /locus_tag="J8I47_000611"
     tmRNA           complement(18310..18676)
                     /gene="ssrA"
                     /locus_tag="J8I47_000611"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(18847..19620)
                     /locus_tag="J8I47_000612"
     CDS             complement(18847..19620)
                     /locus_tag="J8I47_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6322071.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            19764..20114
                     /locus_tag="J8I47_000613"
     CDS             19764..20114
                     /locus_tag="J8I47_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAZ6322072.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            20095..20811
                     /locus_tag="J8I47_000614"
     CDS             20095..20811
                     /locus_tag="J8I47_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322073.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            20811..22259
                     /locus_tag="J8I47_000615"
     CDS             20811..22259
                     /locus_tag="J8I47_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6322074.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(22329..22838)
                     /locus_tag="J8I47_000616"
     CDS             complement(22329..22838)
                     /locus_tag="J8I47_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322075.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(22828..23553)
                     /locus_tag="J8I47_000617"
     CDS             complement(22828..23553)
                     /locus_tag="J8I47_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322076.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(23776..25896)
                     /locus_tag="J8I47_000618"
     CDS             complement(23776..25896)
                     /locus_tag="J8I47_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ6322077.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            26126..27304
                     /locus_tag="J8I47_000619"
     CDS             26126..27304
                     /locus_tag="J8I47_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAZ6322078.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            27320..28078
                     /gene="aroD"
                     /locus_tag="J8I47_000620"
     CDS             27320..28078
                     /gene="aroD"
                     /locus_tag="J8I47_000620"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAZ6322079.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            28101..28586
                     /gene="ybaK"
                     /locus_tag="J8I47_000621"
     CDS             28101..28586
                     /gene="ybaK"
                     /locus_tag="J8I47_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAZ6322080.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(28657..29910)
                     /locus_tag="J8I47_000622"
     CDS             complement(28657..29910)
                     /locus_tag="J8I47_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ6322081.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            30028..31377
                     /gene="gdhA"
                     /locus_tag="J8I47_000623"
     CDS             30028..31377
                     /gene="gdhA"
                     /locus_tag="J8I47_000623"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAZ6322082.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            31489..32835
                     /locus_tag="J8I47_000624"
     CDS             31489..32835
                     /locus_tag="J8I47_000624"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAZ6322083.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(33143..33529)
                     /locus_tag="J8I47_000625"
     CDS             complement(33143..33529)
                     /locus_tag="J8I47_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ6322084.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(33578..>33751)
                     /locus_tag="J8I47_000626"
     CDS             complement(33578..>33751)
                     /locus_tag="J8I47_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAZ6322085.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT        11241 a         5933 c         6709 g         9868 t
ORIGIN      
        1 aaataacctt agaataaaaa gagaaaaaaa gccccaaact ttctatacat caataaaata
       61 ataaaaatta aatttagaat aacgtgtaaa ggattatttg atgatttttt attgagagga
      121 gggatgaaaa tgattaagaa aactcaaaat aaaatccaac gtatatgtca aaaacatttt
      181 gtttctagac tcaaagctgt atctaaaaga aaatacttac atcccattat gatttcgatc
      241 atcttgattt ttactttctt agggagctta tcaagtattt atgtaaacgt ttatacagaa
      301 acgtcaaatt ttgctaacca aacgacagag gagacaaata gtccacccta ttctgaaata
      361 cagtccatag aaagcaccaa tcagtctagc aacaacgaga caacctcaac cacatcctcg
      421 actcaactga ttgatccgac agaaaaagaa atcacagcag caaaagcagc aggggagaaa
      481 gcttattcag aaacaggtag aagccagaca tttagtactg ttgcggccac tgcgaatttc
      541 aacggacata ccataccaga tggatataca gtgatatatg ccaaagatgg agtcattgtt
      601 gcggttaacg gatcgatgag tgcggataaa actgcgttac aaggcgtaac caaagcaatc
      661 gcttttattg ataaccgtcc agatatccat aatctgttta ttggctccac agtaaatggt
      721 actactatca cattcccaaa aagtatctat atgggggtgg ctaccgctgg aaatacgatc
      781 gattcagtta cacccaatgc ggataaaagt tattctttta caccctcaac atggggaggg
      841 cttcctgccg gaggaggtat gagtactttt tcaatcggta tacagttagc aaatgatatt
      901 ccattcacgg ggtactcgat caaaaatggg cagatcgttg atggtaaggg aaatgtttca
      961 aattttccaa cctatcaagc agcaacggtt caggtcaatg cgaatcggac aggaactttt
     1021 tcttatacag tcaatggtgg agcagcaaaa atcggaacgt acgggactac attatctgga
     1081 atccatgtgg gtgacaaagt ggttatcact ccaacagcag taacgggcta ttcttttgca
     1141 caaacaaacg gttctttcac agccctagaa agtaacgtcg atacggtgac ctatacaggc
     1201 aaacaaacac caatcactat taagtatcaa gactattttg gacagatgat tgctccttct
     1261 aaaataatga atcttactta cggaagtgct gcacaagacc tgacgactaa tacgcctact
     1321 atcaacggat atacctttac agctgtttca gcgatagaaa caaagaacca atcagcaatg
     1381 agtgttttag ctagtttaga tacaaatgga aatgtaatcg taaaggacgc aaatggaaaa
     1441 cagatttctg aagtcatcct atattataag acaaatgcaa cagtatcaat caatgcaaat
     1501 ggcagtaagt actatgatgg tctttctgtt gtaccgatcg ttaaatatgc ctttaatgat
     1561 aaaacagaat caacatccct tttaaacaat gatctcagta ttgatcaaat cacatggaat
     1621 ccaactgatt tcaaagcgat gaatgaaaag ggagttgaag taactgcgcc tactgaaatt
     1681 ggaacctata cgtcttggca attgacaaaa agcggactag ctaaactagc tgccagaaca
     1741 aactatttat ttactatcgt gcagacatct gatgtctata cgattaagca aatcagcggt
     1801 gcggtagagc tcagtggcag taagacatat gatggaaaag caggtgtccc gtcaatccat
     1861 gtcaagctag cagagggtgt cactagttcg cttactccag tacaagtggc acttagcagt
     1921 atggattaca cagcagatgc acaatcagca aaaaatatgg ttaatgctgg cagctataca
     1981 attaaactaa cgaatagcgg aatagacaaa gtcaaagcag caaatcccaa gttgtcattt
     2041 actgatatag ccaacacatc aggaacttat accatctata aagctaacgc agtgattact
     2101 gtcgatgacg ctgcctttaa ttatgatgcc caaagtcact cgatttcggt tggaaatctc
     2161 cacgttaccg gtgttgtctt aggcgaatca ttggattata ccctaacagt taacagccgg
     2221 acagatattg gatcacaaat agtaacgatt agtttaggcg aaggaagtgt aaacaataat
     2281 tatacgatta tcgcaacaga taccgctaag ttgacgatca atccgactct cttgcttcct
     2341 tcgacaggtg ggataggtct actgcccttt gtctttttag gactgatctt tattttttct
     2401 ggcttctttt attttatcca tcgtaagaaa aaagcaggtg aacagcgatg aaaaaaacaa
     2461 aaaatgtacg tgtggatata tttgctgttc tcttttttat cctactggtc ttttttatag
     2521 gttctccgac tgtgtcagct agagaatcac ctattacatt tactttgcat aatcgaatgt
     2581 tcgaaagcga taagatgcca agtatcagtc aaaacgacgg aataaatatc ccggataact
     2641 ttcaaggagt aaaagggttg aacggcgtta ctttcgctat atatgatgtc tcggatgaat
     2701 tttacaagct gcggtcagaa ggaagtagtg tggaagacgc acaaagaaaa ctagcacaaa
     2761 aaagtgatag tgaaaaaata ctggcagaaa atgtgacaaa aacagtagac gaggaagagg
     2821 gcattgcttc ttttagtgca tcggacaaag atgagcaagg tcgtgacgct gtttaccgat
     2881 ttacggaaat aaagacttca gattaagtaa aagaaaaatc agcaccattt gtcgttgtgc
     2941 ttcctgtgac agattcatct aatcataaat tgacgaccat ccacttgtat ccaaagagcg
     3001 aacaaaaaat acctgctgca ctcacattaa caaaaacagt agagaataag cagacagatt
     3061 ttgcagatgg ggataaagtc ccttatctca tcaccgtaac aattcctgag aacatcaacg
     3121 agataggaac ctatacgatc aaagatacag ctgatcctca gttatggcta gaaccagaaa
     3181 cgatcgattt tttgattggt ggagacaaga ttcatacatt tcacacacaa aaaataaaac
     3241 atggttttgt cttatcagat tctggaaaag atcttagttc gtttgctgga aaaaaattaa
     3301 cgatccgtta ccaaatgaca ttgaaagaaa acagtgaaaa aacgacattt gataacgatg
     3361 tacgtttgac gactgacaat caaacagtag aaacacatct tgccatccaa acaggaggca
     3421 aacaatttgt caaagtggat cggcaaacga aacaaaaact agcagatgct caatttctag
     3481 tcaaaaaaaa tgaccaatac ttagcgcaag aaaacgggat aaatcattgg gtagccaaag
     3541 atgattcaag agcaacgatt tttacttcca gtaaagatgg aacattttct gtccatggat
     3601 tgtcttttgg aagttacgaa ctagtggaga ttaaaccgcc taaaggatat atattgagtc
     3661 aatcaaccat tctctttgaa gttgaaaaag gcagttatga tccgcttcat cctataccat
     3721 tggaaatcat caacgaacca aaagcaaatc cgacgaataa tggaaaaacg ccagttcaga
     3781 tgcagacaaa tggtccatca aatgaaaaaa atggaggaag ctttccaaaa acaagcgaag
     3841 agatgcttgg aggtttctca attttagggt tattgctagt cctttcaaca gggacagctt
     3901 ggttttataa aaaacaacaa aaaggaaaca ataggaggga aataaaatga aaaacatcaa
     3961 aacaaaaata tggacgtttt taggaactgc tatcatgctg cttcctttcg ttctcggcct
     4021 aggaactgca gaggtttctg cagccgtatc acctacacca gaaaatgtta cggttaattt
     4081 gcataaattg aaattcactt cagcaccaga aaatcaaata aacaacggaa cggaattgac
     4141 atttccgaat agcgaaccgt tgaacggcgt tgaattcaac gtatatgaca tcactgctac
     4201 gtactatcca agtaaagaca cagcagttcc agcagacgct acacccttcg caagtgtaac
     4261 gacttctggt gaaggattag ctaacctgac tcttccaggt aaatctgatg ggaaagatgc
     4321 tgtatacgtc tttgtagaaa caccaaagcc cggtgtcgaa acgtcgccaa atatcgtgtt
     4381 aagcttaccc tagaccctga caaaccttta gacacgattc acctatatgc taaaaatgtc
     4441 atcaaagaaa gcaatatcaa aatccagaaa gctgggaact tttcaggggt agataagtta
     4501 gcaggggcgc aattccagat caaaaattca gagaatcaat tcgtgaccgg ttttgatgaa
     4561 aacactggtg tcgctctcta tgatcctgct gagtcagcgt tgacattcac aactgatact
     4621 gaaggatcca tcagtgtcaa tggattaata gacggcaatt atactttagt tgaaacaaaa
     4681 gcaccagatg gttatcaatt aaccaatcca caaaaagaaa ctacttttat agtaaaaaac
     4741 ggaaaattgc aacaaacaac cggtatcatc catggcgcag atgaagccaa tcttgggaat
     4801 ccaaacggtg tagtcaatcg ggaaaataca actacggata acacgattac tgtagaaaat
     4861 gtccaaaact acggtgattt ccacttcatc aaacaagatg tgaacacgca agaaaaatta
     4921 gccgatgcag aattttctat tctaagagca aaagatccaa ctgttaaatt aatgaaaaaa
     4981 atagcaaacc ttgcctcagg agaataccca tatgcttggt cagatgaagt cacaggttct
     5041 gactgggaag aagtgaaatt gatttctgat tcagaaggga aattttcaat cgaagggctg
     5101 aaacaagggg catactgcct tcaagaaaca aaagcaccag aaggctacgc attacctaag
     5161 aatgaagctg cgtacactga atttacagta gatgatgatc caacaacttc tgatgaaaca
     5221 acgatcgata accaacctaa aggaatcttg ccttcaactg gtggtatggg gatcatcgta
     5281 ttcatcttgg taggtactgc tttagttgga ggagcagtga tctatttcaa aaaacgccat
     5341 caagaaacag aagcttaaac tattggaaac aaaaatcata cgataaataa acgatagtga
     5401 ttcaaatggg gagaaaggtt gtgacaagtg gtcggtctct tggtattcgc tcaacaaacg
     5461 gcaaaatagg aggaactatt ttgccgtttg cttcgctaat actcaggtca aggcgctgat
     5521 ttcacaacct cttcatatac atattagaaa ataggtggac atgatgaata aaagcagaat
     5581 aagaatactg gacatcttca tggcaatcat tttagtcgtt gggatcggga tcttttctta
     5641 tccttttgta gaagattcac tgaatgattt tcttgcccag caaatgatca tccattatca
     5701 aaagcaagca tcgaataaga attcagagga aataaaaaaa cagcaggaaa aaatgacaaa
     5761 gaaaaaccaa cagttggcag agcaaaacgt atcaccaggg ataggcagtt tcaaccaagc
     5821 agttgatgca aaagcattaa aagatttacc tagtaatgcc ttttttatgg aacatatgct
     5881 tggagtcatc gaaatcccaa aaatcaatgt cagcttacct atattcgatc agacaacgga
     5941 aatatttttg caaaaaggaa cgtcattatt ggaaggaagt tcttatccga ctggcgggaa
     6001 aaacacccat gccgttcttt ctgggcatcg tggactccca gaagcaaaat tatttacaga
     6061 tttgcctaag ttgaaaaaag gagaccagtt tttcatccag atcaatggaa agacattggc
     6121 gtatcaagta gaaaaaatcc aagtggtctt acctgacgaa gtagattctt tgggtatcca
     6181 aaaaggcaga gatctcgtga ctttgcttac ctgcactcct tatatggtca atacacatcg
     6241 actgcttgtg acggggcatc gtattcctta tcaagcaaaa gaggcaaaaa aagcgatcca
     6301 aggaattgat caatggaaaa aatggaaatt ttttatttgg tttatcggta tactacttgg
     6361 atcaatcggc ttagtttggc tactcatcgc ttatttagat tctttggcca tcgctaagcg
     6421 aaactatcct ttatcttttt atgtaaagaa cacaaacgga cgaccaatcg aaggaatggt
     6481 cttttctgta aaaacgctca acggcaaaca ctatattact agagaaaaag ttccatttgt
     6541 gaaagcttct gatgaatacg gactagtgag attttctgat ttaaaaggca ggaactatcg
     6601 cttgcaacat gaggagctcc tgcttaaaat ccatgtaaaa cataaacatt cgaaacagtt
     6661 ttcaatgaaa ttgaaaaaag gaagatataa actgcgaaaa gaaaaagagg tgtactattt
     6721 gattgagaaa gaatgaatga agatgtccaa aaaagcagag ggaacagact aaatgaaaaa
     6781 agagagaagc tgacgaaatg gacgtgtaaa ctgctatttt tggtttacta tctggaacgc
     6841 cagcttctat ttttttatgt ttcagaagaa ttttattatt atagtataac ttttgaatta
     6901 gtttccataa catttggagc aaggggttaa accacgagct tgagcttcgc ttaaagtggc
     6961 aggggtatac gtaccatttc cacatttatg tgtatggtat tttgaaccgg tcggtgtgac
     7021 atagacagtt tgttcattat tttgctcagg agctgcggct tgtgcctgct gagaagcctg
     7081 ttcagctgct gcttgctgtc gcgcctgttc tgcttgttgc tgtgcgattg cttcttgcct
     7141 cgcctgttct gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg cttgttgtcg
     7201 tgcaatttct tcttgctttt tccgttcttc ttctgcttct tttgctttga ttgtggaatc
     7261 aattgcagca agacggcttg tcaactcttg tttgttttca ggtagcgctt ggatagctgc
     7321 tgaagcagtt gcatagtttt cttttgtagg atttgcttca gcttgtgaca aagcagattc
     7381 cgcttctgtc actttttttt ctagtgcagc tttttccgct ttttgtttct cttcttcttt
     7441 tttcttttca atttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct
     7501 gctggaagcc tgagctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact
     7561 caacaaactt cctgcgacca aaaacaacgc aactaatttt ttcactaaat ttcctcctta
     7621 agtaaaaaac aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat
     7681 aattgtgtta tgaatagtga ttgaggaaac ttaatctaat cccgtgttga tacacgggat
     7741 tttttgctat agttctgtta ttaagtatgc aataataaaa tatgtacgtt attttattat
     7801 tagattattg acatttatat aataaacgat taaatgattc atatcaatag aatacttagg
     7861 aaaaaaagag aaataataac aggtttatgc actattgacg gtgtgacaat atgatatttg
     7921 tatccgtttt tttcttgtat gtagtatagt aaagtagata caggaggaga aaatatgcag
     7981 acttttttaa caaaagtttc tcaaaagaaa attaaaatga ttcatttact gcttgaaaca
     8041 gaacgttggt gttcaataga agaactacaa aacgaattga aggtatcaag taagtctatc
     8101 ttgaattatc taacagattt agaagaattg ttccaacaat atcctgataa ggtcattttg
     8161 aagaatgaga ataatcggcg cttttctatg gaaaagcagg agaacttccc aatctacatt
     8221 atttatcttc atttctacag aaaatcatat aattatcatt tgattgaatt catgtatcaa
     8281 catcctgaga aaaatttgga agattatgca gacgctcaat atacgagtgt atccacagtt
     8341 tttcgttatg ctaagttatt agtaaagtac tttgaaatga cggacgtcaa agaatagtcg
     8401 ccgccagata gaaggaaatt agcagttgaa aaagctgccc tgcatcaagt aatacgacca
     8461 gccaaacgga aacagtgtct catgtttttg atttggctgg tcttattacc gcagatgcaa
     8521 gctttttcca cacctctttc ttgtcattta cagactcaac tatcaatgct gcaagctttc
     8581 ccttaatgct gtaacaccag taagagttgt caagaccagc agactgacta ggtaaatacc
     8641 gctcaaaaac aagattacgg cagttaaact ataatgatcc atcaagaaac cgatgacgat
     8701 agaagaaaaa ccaccaacag ctcgtccaag gttcataacg aaattattcg ccgtcgcgcg
     8761 gatttctgtt ggatacaaac tactaatcac tgcgccatat ccaccataca tgccattgat
     8821 aaagtagccg actatcacag cggctgctac caaactccac tggctatggg ctaacacaag
     8881 tagatagaca gataaggatg aacaaattaa aaacaaagaa aaggctagtt taggccccaa
     8941 tttatccatg attatgccaa aagtcatcat ccccaaagac atgcctaaaa tagtactgac
     9001 catccacaat gaagacccag aaaccgaaag ccctaatcga ttttgcataa ttgaaggtag
     9061 ccaattcatt aacccaaaat agccggcaat ttggacagtt accatgatac taaggccaca
     9121 tgtttgccaa gcaagatgtt ttgattcaaa cagtctgcta agtttggttt tcttttcggc
     9181 agattgtaca gatttgaatt cctttggttc agtcaagccg cgacgaatga acaagaccat
     9241 caacaccggc agtacaccta cgacatacaa catcttccaa ccaaacgttg gaatgatcac
     9301 agcagctaaa atcgcagcca aagccgcacc gacctgcgca ccaataccag caaccgaaga
     9361 agctcgtcca atttgttttt ttgaaaaact ttcggaaatc aaggacatac acgccccata
     9421 ttctcctccc gctcctacac ctgcgataaa gcgaaagaga taaaccaagt agatgtttga
     9481 agcaaaatac atcaataaag aagcaatcga aaacagaagg accgtttgag aaaatacttt
     9541 cacacgtcca tatttatcag ctatgatgcc aaaaaaaata cctccaatga gcatccccaa
     9601 attagtgatc gttgaaatga aacccgcttg cgtaccacta atatgaaaag aagtaatcat
     9661 tgaagataat gaaaaagcta aaaacataat atccatattc tctaaagcaa ttcccgccga
     9721 tgtcgatact aatacctttc gctgatacgt tgtcattttt ataccccttt tcctaaaatt
     9781 tccctaaatt gttaaaagta tatccaaaaa agaataaaaa aacaagactt ttttatttga
     9841 aataatacac aaacaaccga tatagataat ccagcttagt tttaatagaa atgagtattg
     9901 acttggagta cacttaaagt tgtagaataa ataaaataca taagtaaagt ggcaagaaat
     9961 taagagaaag agtgatcaga tgaatatcaa acaagtcagt gaagaaaagg gcatttcggc
    10021 cgatacattg cgctattatg aacgaatcgg gttgattcca ccagttaatc ggacaaaagg
    10081 cggaatccgt gattatacag aagaagacct aaaatgggtc gactttacga tctgtatgcg
    10141 aaatgccgga ttatcgatcg agtctttgat tgaatatatt cgcctatcta gcgaaggaga
    10201 agcaactgta tttgagcggc gtaagctttt gattgaagaa agtgaacttt tgacgaaaaa
    10261 aattgctgaa atgcaagaat gtttggaacg tttacaagga aaaatcaaga aatatgatcg
    10321 tgtgttatct agaaataaaa tggaatgtat caagtaaaaa aatcaaggag catcgctatg
    10381 gagttacgct tactaaaata tttttggaca attgcagaag aaggaacaat ctctcaagcc
    10441 gcagaagttc tacatcttac acaaccaacc ttaagccgac aactgaagga gttagaagag
    10501 taactaggaa cagaactgtt tgtacgcgaa aaccgcaaaa tgatcttgac agaagcaggt
    10561 tattttctaa aaaagccgag cagaagaaat tttagattta acgaatcaaa ccactcaaga
    10621 attcgagaac agaaaaaaac aattattcag cggccgattt acgatcggct gtgtggaagc
    10681 tgatagttcg aatacattgg cactattact agaaaagttg atccgtgatt atcccagcgt
    10741 tcaatttcat atccacagcg gaacaagtga tgatatcacg gagtgactag aaaaaagcct
    10801 gttagattta gcgattttgc tagatccagt agatacagag aaatacgaaa cgattttgtt
    10861 accaagaaag gaaaaatggg gattgctcat tttagaaaat tctgttttag caaaaaaaga
    10921 cgtgataaca aaagaagact ttgcgaatgt acctcttttg atctcaaagc gtccagaaat
    10981 acagcgaatg tttgctgact ggttaggcat aaaaaaggag cagttgctga tcacaggaac
    11041 gtacaatttg attttcaatg tatttgcgct aattgagaat caggtaggtg ctgctttagt
    11101 catagaaggt gctgtaagtc atcattcaaa gcgagtgaca ttcgttccgc ttgacccaat
    11161 gattgaaacg aactgcatgc tagtctggaa aaagaatcgg attttatcac ctgtagtcaa
    11221 tgaattgatt caccgtttca aagaagcttt tgaacagtaa taagtatctc taatcgaaga
    11281 aatttcaata caaaagcgag acgtatgaca gtcttgcttt tgtatttttt catttttaaa
    11341 tcaaaaaaat aaagagattt acataacttg ttaaataagc gcttacaaaa ataagaagaa
    11401 cggaaatgaa tcagctataa taaccgttgt tctggattgt aatcattaca aagaaatgaa
    11461 gattataact ttggaggtgt tcgaaatgaa agaaaaaaag caaaaattcg ttcaaaaaac
    11521 atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt attgacgatc
    11581 attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt gttcacgatt
    11641 ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat ttggtctcgt
    11701 attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat cacgttgatt
    11761 cagttgttta cttctagttt tgctgcttac gggttttcta aactaaattt taaagggcgc
    11821 gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc ttatatgatc
    11881 ccgcagttca ttatgatgag aaaagtgggt ttaacagata cgttgtggtc gttggtgcta
    11941 ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatattacag tagtatccca
    12001 gattctttat gtgagagtgc gcggatcgat ggcttatcag agtggggaat ctatcgcaag
    12061 atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac attcgttaat
    12121 acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa taaaacaatc
    12181 cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt aatcatggct
    12241 gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca aaaatatttt
    12301 gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa agagcagcgt
    12361 tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt ggcaagtagc
    12421 ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt gtcgatagat
    12481 ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg ttgcatcgtg
    12541 atgataggta tgacagaaga attagcaaaa gaaaatagtt tttcaccagt acgtttgttc
    12601 ataaagatgt ttcgccaatt ttggctaaag ggattttttt attggctatt tgcttggata
    12661 gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta tggaaaatgg
    12721 ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcac caactgctgg
    12781 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    12841 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    12901 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    12961 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    13021 gaaactttca attaagaatg acagtttttt ttatgtaaaa actgtcattc tttttcattt
    13081 tttgaaacat atggctaaac aaatgaaata aaacgtgcta tacttttgta tgaaacaggt
    13141 ttagaaactg tatcacttat ttgtgattat ttctaactaa taattaaaaa taaagcataa
    13201 aaaggacgat gttcaatggc tgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt
    13261 ttgattaatc atcatgttga ttatgtattc ggtataccag gtgcaaaaat cgatggtgta
    13321 tttaatgaat tagaggatca aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct
    13381 gcatttatgg cacaagcaat cggacgtatt acaggagagc caggagtagt tatcgcaaca
    13441 agtggtcctg gtgctagtaa cttagctaca ggtttggtaa cagcgacagc agaaggtgac
    13501 ccagttttag caattgccgg acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa
    13561 agcatggata atgcagcact gttccagcca atcacaaaat acagtgcaga aatccaagat
    13621 ccagaaacaa tttcagaagt gattgccaat gcttatcgta tggctaagag ttcaaaaaaa
    13681 ggcgcgagct ttatcagtat cccgcaagat gtagtggatg cacctgtcca aggtaatgtc
    13741 atcaagccat tgagcgatcc taaattaggt tctgcttctg cagatgatat ccgttaccta
    13801 gctgaacgca ttagagaagc aaaactaccg gtactattag taggtatgcg aggatcaagt
    13861 gaaaaagaaa cgttagccat caggcaatta gttgaaaaga cagcattgcc tgttgtagag
    13921 acattccaag cagctggagt gatctcacgt gaattagaag cgcatttctt tggtcgtgtg
    13981 ggtttattta gaaaccaacc aggagatatg cttttgaaac gaagtgatct agttattgca
    14041 attggctatg atccaatcga atatgaagca agaaactgga atgcggaaaa agatgcgcgg
    14101 atcattgtga tcgatgaagc gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa
    14161 ttgatcggtg atatctcagc cactcttgat ttattgacag gttcattaga gcctcaacaa
    14221 gtgtcagaag atgcaaaaga atatttagca tctcttcaag caaaattaac ggagcgggac
    14281 attgttcaat caaaaggaga agcaggtatc ttgcatcctt tagaagttat taacacgctt
    14341 cagtcaaaag taacagatga tatgactgtt accgtagatg taggtagtca ttatatttgg
    14401 atggcacgcc actttagaag ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa
    14461 acgcttggtg ttgcattgcc atgggcgatt tctgctgcat tggtgcgtcc aaatacacaa
    14521 atcgtatcag tttccggcga tgggggattt ttattctctg cccaggattt agaaacggcg
    14581 gtacgtaaaa aattgaatat cgttcatttg atttggaatg atggacacta caatatggta
    14641 gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg tagactttgg tccagttgat
    14701 tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc gagctacaag tgtagaggaa
    14761 ttagaaaaag ctttagaaga aggatttgcg acagaaggac ccgtaattat tgatattccg
    14821 attgattacc gtgataatga gaaattggga gaaacaatct taccagatca attctattaa
    14881 ggaagtgtcg acatgactga aaaaatctta taccagcacg ggacattagg agctttgatg
    14941 gccggcttga tggatggaac agaaacgatt gctcatatat tagaaaaagg aactctggga
    15001 ttgggaacat tgcatgggct tgatggtgaa gtcatctttt tagatggcgt agcttatcaa
    15061 ggaagatcag atggctcagt tgcccaatta gatggaagtg aacttacccc ctatgcagca
    15121 attacagatt ttacgccaga tactagtttt tctgtctcaa aaactgcaga tggagaacaa
    15181 ttgaaaaaag acatattagt aagagaagcc ggggaaaacc tatttttggc agtgaagata
    15241 actggattat tcaaaaatat gcacatccgt atcatgccga agcaagaaaa accttacaga
    15301 agactagcaa aaatttcaga gtctcaacca gaattccaac aaagcaatat ccaaggaaca
    15361 ttagtcgggt tctttacgcc tgaattattc caaggagttg ctgcagcggg atttcacctg
    15421 cattttatcg atgatacaca tacatttggc ggacatgtaa tggattttga agtggctgaa
    15481 ggaaaagttg agattagccg catctcgtcc cttgtacagc attttccagt tgatgatccg
    15541 acttttgttc acgaaacgat tgattatgct gatttagcag cagaaatcca acaagcagaa
    15601 tagaaaaaga cagcgagatg cgttcaaggt gattaacctg acgtatctcg tttttttgag
    15661 gaggaaacct agcgtttaca aaaaaactga gctataatct tctccataga tcaaaaagga
    15721 gcggttttta tcatgtggac agaaaaacag aaaaaagatg catacgaaat tttattatta
    15781 cagcaacaag gattattaga agcagaagat aactggttag caaatttagc aaattcatct
    15841 gcattgttga atgagacttt gcctgagact gtctttgcag gatattattt attcgatggt
    15901 aatgaattga ttttaggacc gttccaaggg cgagtttctt gcacacgtat caagatggga
    15961 aaaggagttt gtggagaatc agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa
    16021 acccatgcaa attacatttc ctgcgattca gccgcacaat cagaaatcgt agtgccaatg
    16081 gtaaaagaag gtaatttaat cggtgtttta gatatcgatt gcggagtaac gaaagggtat
    16141 gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc ttttattagc aaattttaat
    16201 ataaatgctt tttcatttat taataactaa aatcgtctat tgaaacaaaa aagaagagaa
    16261 atcaagaata aaaagagccg gaaagacagt ggtcatttct gtttttcggt ttttttattt
    16321 ttttataaaa tcacaagtgt tattgatttt cacaatcata ttttaatggt attatcattt
    16381 tgaataatga aaaaacatta aggatggatg aaatatgaat aacgaaacta tcattttagg
    16441 aaaaaaatat atttgccaac ctattggctt gaaacaaaaa gtggttggag aagtaatcag
    16501 taaattagaa aattgtgttg tgatctgcgt agacgaatat catgacgtcg atcgcaatga
    16561 aatattagaa aaatgcggaa aagtagtggc aaaatatact cacgtttatg agttggaaac
    16621 aacagaatat ctatataact atagcaacaa atatgaacat actttagttt cataaagatt
    16681 taaataaagt gccttcaaaa gcgagaagac caagcttttg aaggcacttt atttagttgg
    16741 cagtgattgt tcatgatttt tgatttattc aaaaggttcg cctagagaat ctgtctctgt
    16801 aacgaggcct ggtggagaaa ctcgataaaa acctatactg ccagaactgt cctattttcg
    16861 gatagacaaa ctagtagttt taaaaagatc atccgatcca atcatttcaa aaaatgcaaa
    16921 actgatatca ggatcattta gctcaggctt gttatcgtaa agcatttgta aaggttgcat
    16981 ttagttagga gtaaacgcga aactatcatc attatttgct gaagaagatg aagaagctaa
    17041 ttcagagttt gtttgaaata aaggagacgt gctgacagtt tctttaccct gttttttgca
    17101 gaggtagaaa cttttatatc ggaagtttct accggagaat ttttctcatt tgtagaaatt
    17161 gttgaatcga tactattcga aatttgcgta tctgaaggta tgttctttga tgcagaagtc
    17221 ccacaagcag taagtaacaa taaatttgat aaactgataa acagaattct tttcattatg
    17281 tacacactcc ttatagtatc gtttagcata cacctatgct cagtattagg caaaatcaat
    17341 gcaaaactat caagtctttt atagtatcta ttagtcttag taatacatat atggagaggg
    17401 tgatatccca gttcaaacca gatacattgg ttaccatctg tattaacgat tatgcctcta
    17461 aacgcttttt ttatgaaaaa atttataagt gaaaacgtag gtatgatatt atctagaggt
    17521 aatttttttg ggtatttaaa tagttgctag gaggatagct tttggatttt tacagtaatt
    17581 ttatcttaat aatagcaata ttattacttc taaatatttg gttttttgat aagagtagaa
    17641 atgcagggat aggttttaga accaaacgaa gtacgtcttc tgaaaaaaag tgggtatatt
    17701 cacagactat tttttacgga ggggttattt caatcagttt actttcttca acactttatt
    17761 cttttaatgt tatagacgtt tccatgtcaa attttatatc tattattgga atccttattt
    17821 ctgcaattat tacacaacta cttcttgtgt ttgaagagaa gtcaaaaaat aattaaagca
    17881 attatgcctt ttagtacagg aattattact tcttacaaat gtatagcgaa tgtgaagcct
    17941 gctgttttaa ttgttctaga aaaacttttg agtgaacaat tattttatgt taggatgaat
    18001 taatttctct agagtttata attaaaaaac ttatttgaag gaacatgctt aaaccataat
    18061 tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtgtacg aacaaaagtt
    18121 ctttttgtaa accttaaaat agattttcct gaatacgtat tggggatttc taaatgtttg
    18181 aatgtttttt tgaatgcaaa atcaatgcaa aaaattttgt atttctctac ttttctctcc
    18241 ttgaccaaaa taaaaaaacg ttgatttaac aacgtttttc atacatgttt atctgtctca
    18301 accatacata tatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
    18361 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
    18421 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
    18481 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
    18541 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
    18601 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
    18661 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
    18721 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
    18781 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
    18841 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
    18901 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
    18961 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
    19021 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
    19081 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
    19141 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
    19201 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
    19261 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
    19321 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
    19381 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
    19441 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
    19501 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
    19561 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
    19621 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
    19681 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
    19741 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
    19801 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
    19861 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
    19921 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
    19981 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
    20041 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
    20101 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
    20161 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
    20221 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
    20281 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
    20341 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
    20401 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
    20461 ttggcagaag caagagaaga agcagcagtt tcagatgatc attttgatat cgagactagt
    20521 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
    20581 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
    20641 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
    20701 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
    20761 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
    20821 tgtatcagca actcttagct ttttgcggaa tggtcgcgtt gatcttattg atcgttggag
    20881 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
    20941 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
    21001 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
    21061 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
    21121 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
    21181 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
    21241 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
    21301 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
    21361 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
    21421 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
    21481 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
    21541 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
    21601 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
    21661 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
    21721 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
    21781 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
    21841 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
    21901 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
    21961 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
    22021 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
    22081 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
    22141 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
    22201 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
    22261 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt tactataaaa
    22321 tgattgattt agcccgcggg ctctaaacga tcaatataag gactttgagc aatagtagca
    22381 tcatgagtaa agagtaagat accttgattt tcttcatgag ctaaataaga aagcaagtgg
    22441 agtagctctt ggatctccaa tacactaagt tcatcaaatg tatcttcaat aacaatattg
    22501 tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta ctttttcaaa agacgtcaac
    22561 tcagctgccg cacaattaag caaggaagga gagatatcaa gtttttcttg ataggccaaa
    22621 cgatcttgtt tgatttcttt ttccggtaca tccaacagga catggtcttt aagattcaga
    22681 taaggaaaca tcggccaatt tttttggatg atcgcaaaat tatccagttt tgcaacaaga
    22741 acctcttttt tcaagtgctc caatatttta tgctgttttt cgagaggaca caaaataacg
    22801 gtcaagtgtt cagcatgaat caaatattca ggtttcatta cgtccaggcc tcctactttg
    22861 caaatgatgg ataaaaaaag tgaaagggaa tagactaaga atgagacagc atccactgag
    22921 cccaataaga gtttgggcaa atcccttaag tatatctgtg aaaaaaagat cattttggaa
    22981 atcgatggta aaaaaagtct gttgatccat cggaattgta agtccgctta ccgaagtcaa
    23041 gatatcacgg atatctggca gttcagtagt ctttaagaca agaaaattga tcctttgaag
    23101 aagcgcttca taaactgact ggaagcttac gatccacaat aaaaccaaga tacaacaaag
    23161 aaagagagga aagaacagct gccagaaaat aaaaaggaat aatttggttt tagaaagacc
    23221 agtcaggcgc caattgatca attcctgctt gttttgcctg actgaatagt aaaagaaaaa
    23281 gataaaaagg aaggcaaata gaaaaaataa actgaatatg ccttttatat aaaatcggct
    23341 agctaattct aaagccggaa gaagctctgt agatgcagaa gcgccaataa atgaaagccg
    23401 atcttggact tgattggcta aagccgattg caaatggtaa agagtcaggc atgctatcaa
    23461 taaaaaagca gaaaagcttg aaaataaaca atagagtatt gtctgctttt tgtaataaat
    23521 taatcgagca gtagcatagc ggattatttt catacttttc acctctataa aaagtataga
    23581 aaatgaatat gaataaagta tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa
    23641 tattttgtaa aaagcaagag gttgtgaaga agctgcccta ttctattacc gtagatgcaa
    23701 gcttttgaac actgtttatt cgaaaagagg agcagcgaca ataattttgt cgctgctcct
    23761 tttaaattta ttttgtcatt cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt
    23821 ttgcgtttta taaagatcaa cagttgattg tccgttgtgt tcactaaaga cactagttga
    23881 cttgtcacca agagattttt cagcttcttt taatttctct aagccattct tgtagtcata
    23941 atcactggta tcaattgggt ctaatccact attcgtatag aaccgaagaa gatcaccatt
    24001 attgatttgg tcagaaacag ctaactgttt atcaactttt tctttcagtg aatcaatttc
    24061 attctgtact tcctcagtag gttcagtaat agcttcacca gtttcgttac tatagatcgt
    24121 accaccgtag taagtatatt ttggtgtaac aaaatcacca tcacggaaag aaacaagctg
    24181 ttcatgatct ttcgagaata gatcttgtcc taactgaata taattttttg tatctacacc
    24241 tagtaagtga agtaatgtag gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc
    24301 gccctgtgtt tgtccaggga tatggatcat ataagggaca cgctgcattt gtgcattgtc
    24361 gtagtttgtc caagtagaac ttgtttttcc aactaattca gctagatttt tatttcttga
    24421 attcgagaca ccgtaatggt caccgtaaag gacgatgact gaattatcat acaagccgct
    24481 ttctttcaaa taattgaaga attcttgtac ggcggtatct aaataattgg ctgtcgcaaa
    24541 gtaaccgttg atcgtttcat catttgtatt ggctaacggg aatcctgctt cgtcattggt
    24601 gaattttgaa taaggataat ggttagatac agcaatgaat tttgaataaa atggttgttg
    24661 cagacgttcc aagtattgta cggattgttg gaaaaatggt ttatcatgca acccgtattg
    24721 gaacgaattg tcatcattaa catcataata agaagaatca aagaaataat catatcctaa
    24781 attcttgtat gtctcgtttc gattccagaa tgtaccagcg ttcccatgga ataccgcact
    24841 agtgtaacct tgtgtttgac ttaaaatatc aggtgcagct tggaaagtat tttttccgcc
    24901 taattgtgtg aacaaggagc cttgattcaa gccaaacaat gaattttcca tcagcgtttc
    24961 cgcatcgctt gtttttcctg cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct
    25021 gttgctgtgg taaagactat tgatgaaagg tgtaacttca tgctggagac cattctcatc
    25081 tgtcaattta taatcgatca ggaattgctg gaagctttct aaatggatat aaatcacatt
    25141 ttttccttta gcgagcccga acaaatcgtc atttggtgct gcataatgtt cttttacata
    25201 attttctact tctttcatat cattgggact tgcttctgcc cgcacttggt ttgtctggta
    25261 agtttgaaca ccgtcataaa ccatgaaagc attcaagcca agatatttga ccaaataatc
    25321 acgagagaat gttcgagtca gcaattctgg acgatcagct tccgccataa aaagatttcc
    25381 tgagaaaatc ataactgcta atgtagagat cgcaaaagcc attcttgctc ggatcgggcg
    25441 agggtccaac ttgatttttt ttgcaatcag cagaacagtc aagataataa aatcgatcca
    25501 atagatcaca tcatatgggc gaaacagtcg aatggcactt tctcccagac cactcgctac
    25561 tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc gtgaattctc gataataagt
    25621 gacattcgaa aaaacaagca atgtcaaaag aaaataaatg accatcatcg tgatataagc
    25681 aactctaggg cgtctgatat aaagagcaat tccaagtaat aacaatgtag ttgcaatagg
    25741 gttgataaac aagataaagt attgaatgcc actttcgatt ccgagatgga aatcaactaa
    25801 ataggcaaaa atcgttttca accaaagtaa aacgactagt aaagaaaaaa atccgagcct
    25861 cttattcaaa aaggcaggtg tttttataca tttcaagata atcttccttt ctgcacttct
    25921 taatcacaga agtgtcatga aaccatgtta cacagctcac ttattctcat ttattttact
    25981 agtatgacaa tatagtgtca attgaaacca gaagtttatg ctgtttcatt aaggttttct
    26041 ttaagcttca tgaaaaaatg agcagtaaga caagtcctat gttttttgtt atacttggga
    26101 agagaaagct aaagcaggtg aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg
    26161 attgaaaaaa ggatatccac tgatccaaaa agaagacctt gctcatcccc aaaaagaaac
    26221 cacgcaatgg gtggaattag ttgattcgca aaaccaattt atcgcaaaag gatatctagg
    26281 aaagcagaat aagggaatcg gttgggtatt gagccagcag aacatcccat ttgatcaatc
    26341 tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga actttctttt tttcagatga
    26401 ttctactacc gcttttcgta tattcaatgg agaaggagac ggaatcggcg gtctgattgt
    26461 tgattattat gcaggttatg ctgttttttc ttggtataac gaaacgttat atgcacatcg
    26521 tgaattattg attcaagctt ttcgtgaaat ctacccggaa atcgtaggag gctatgagaa
    26581 aattcgattt gaatcagtaa atctgcctga gagccagttc atttacggaa aagaggcacc
    26641 ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc gtttatttaa atgaaggatt
    26701 aatgactggg atctttttag accaaaaaga agtacgggga tcattagcag aaggtttcgc
    26761 ttctgggaaa acagttttga acatgtttag ttataccggc gccttttctg ttgctgccgc
    26821 aatgggaggg gcaatcgaaa caacaagtgt cgatctagca aaacgcagtt taaaaaaaac
    26881 acaggaacag tttgaagtga atggtttgga tgtcgatcag caaaaaatca ttgttatgga
    26941 cgtattcgag tatttcaaat acgccaaaag aaaacaattt ttatatgaca tgattgtatt
    27001 ggatccacca agttttgcca gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg
    27061 ggaactggtc aaggatagtc tggatatttt agcagaagac gggatactta ttgcgtctac
    27121 aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg attgaagagg aattccatgc
    27181 caaaaatgtt tcttttgaac aacttgcttt atatcgtctg ccaaaagatt tccagacgac
    27241 ggataccttt ttagaaggca attatctgaa agtgtttgtt tatcaaatta aaaataacca
    27301 ttagaaggag cgatgcagaa tggtttgggt aaagggattg aatataggaa gcggtagacc
    27361 aaagatttgt gcgcctctta tcggggccgt ccgagaggaa gtattacgtg aagcagttct
    27421 tgcacgtgaa gcaggggccg atttagtcga atggcgagtc gatcattatc gtgaagtttt
    27481 ttcttttgat gaagtagcag aaacattggt tcttatccat gagatgcttg atggactgcc
    27541 gttattattt acgtttcgta ccttagctga aggcggagaa caagacatat cgatccgtca
    27601 gtatcgcgac ctttatgaac atgtgataca cacaggatta gtagatatgg tcgatatcga
    27661 gttgttcaaa gtagaaagtc taggaaaaag actgatagag gaaatcaagt ctttaaacat
    27721 tcctttaatt atcagtagtc atgattttaa agaaacacca gcagaccctg tcttgcttta
    27781 ccgattgaat gttatggaac attttggagc cgatatcgga aaactggcag taactcccaa
    27841 taatgaacga gatgttttgc ggttgatgga gttgactaga cgggcaaatg cttttgtctc
    27901 catgcctatt attacgatgt ccatgggaaa tcttggtaaa atttctcgtc tggctggcgg
    27961 aacgacgggt tccgttatga catttggttc cttaagcagc gattctactt ctgctccagg
    28021 acaattaccg gtcaaagcat tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa
    28081 caagtttgag gtgatatttt ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta
    28141 tggtcgaaca aaaaaagatt ccctatacag agcacgaata tgagtgggac gaaagccacc
    28201 taagtgcgtc atcggtcgct gaacagcttc cagaaagcca atctcgtata tttaaaacac
    28261 tggttgctgt gggcaatgtg acggggcctc ttgttgctgt gataccggga gaagccgaat
    28321 tgaatctaaa aaaattagca aaagtttcag ggaataaaaa agtagaaatg ctccatttaa
    28381 aagatttgga agcaacgacc ggatacatac gtggtggctg ctcgcctata ggtatgaaaa
    28441 aactatttcc tacttattta gatcaaattg cagaaagcta tgaacaaatc attgtctcgg
    28501 ctggcagaag agggctacag atggagttag cacctcaaga tatctgtgca ttaacgagcg
    28561 gacaatttgc agatataaaa caataaatca tacgtataaa agaaagaagg aggccgggat
    28621 gttgtttgtc cagtctcctt ctttcttttt tatacattat gctctttgcc cggcattcat
    28681 tcctgctaca tgccctgtag cgaaagccgc tgtgatatta aagccgccag tataaccatt
    28741 gacatccagt aattctcccg caaaatacag accatcaatg attttactct ccaatgtttt
    28801 aggataaatt tcctttagag aaacccctcc gccagttaca aatgatttct caataggaaa
    28861 agttttgttg attgatagct taaactcttt gcatagttga acaaaatctt gaatttgttt
    28921 ttgagaggct tgttgtcctg tcagatgatc catttcaagc cgctcaagaa agaaaactaa
    28981 caatcgctca ggcaatatac catgccaagc attgaccaag ttctttttgg ttgctttact
    29041 tttttctgtc aatatatgaa taagctcttg tttggtttgt gtagggaaac aatccagaga
    29101 aagtgtcact ggttctcccg ttttctctaa ttctttattg acaaaacttg aacaacgcag
    29161 tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa
    29221 tacacgacct tttttattta aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag
    29281 tgttttctcc tgaatatacg gttcatccga tatcaagggg gactctgtcg cataaagagg
    29341 cgtcaccgtg tgacccactc gcttcacgat cttgtaccca tctccagtag aaccagtaga
    29401 tggataagtc cgtccaccag ttgttaagat aatggcagga gcatggaact cttcgaattc
    29461 cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat ttctctactc gagtagagaa
    29521 tagaagcgtc acatctagtt ctttcagacg attgagcaat gcttcgataa tcgtttttga
    29581 tttatttgtt acaggaaaca tccgtccatg atcctcttct tttaaatgta cgccttgtga
    29641 ttcaaagaag ttcatgatat cgaaattatt ccattgcgca aacgtactat ataaaaactt
    29701 tccatttcca ggaatatgtg caatcaaatc atcaacagga cgattgttcg taacattgca
    29761 ccgaccacca cctgtcatca gtagtttttt tcctgctttt ttgttttttt cgatcaagag
    29821 tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc atcccgcttg ttccagcgcc
    29881 gactacgatc acatcataca gtttttccat tccctcacct caaggaaagt gtagcatatc
    29941 tgaagaaaga aaacagaaaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
    30001 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
    30061 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
    30121 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
    30181 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
    30241 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
    30301 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
    30361 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
    30421 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
    30481 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
    30541 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
    30601 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
    30661 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
    30721 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
    30781 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
    30841 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
    30901 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
    30961 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
    31021 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
    31081 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
    31141 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgtccagcga
    31201 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
    31261 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
    31321 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
    31381 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
    31441 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
    31501 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
    31561 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
    31621 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
    31681 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
    31741 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
    31801 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
    31861 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
    31921 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
    31981 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
    32041 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
    32101 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
    32161 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
    32221 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
    32281 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
    32341 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
    32401 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
    32461 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
    32521 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
    32581 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
    32641 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
    32701 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
    32761 aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc taaagaattg
    32821 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
    32881 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
    32941 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
    33001 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
    33061 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
    33121 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
    33181 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
    33241 agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta atgacatcga
    33301 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
    33361 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
    33421 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
    33481 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
    33541 ttttttattt ttattatacc cgtggtgcta gttaatttta atatcctaaa gaatatctca
    33601 aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc agtaatatgc
    33661 tttcaaatct actctctagc cctttaacag aacgtgagtt gaaattttga caacctaatt
    33721 tttctaagga agaaaaaaca gtctcaactg t
//
LOCUS       DADOZM010000015        31841 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.015, whole genome shotgun sequence.
ACCESSION   DADOZM010000015 DADOZM010000000
VERSION     DADOZM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.015"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(151..2304)
                     /locus_tag="J8I47_000627"
     CDS             complement(151..2304)
                     /locus_tag="J8I47_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAZ6322086.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="J8I47_000628"
     CDS             complement(2598..3446)
                     /locus_tag="J8I47_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6322087.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="J8I47_000629"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="J8I47_000629"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAZ6322088.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="J8I47_000630"
     CDS             complement(5947..6126)
                     /locus_tag="J8I47_000630"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAZ6322089.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="J8I47_000631"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="J8I47_000631"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAZ6322090.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="J8I47_000632"
     CDS             8274..10553
                     /locus_tag="J8I47_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6322091.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="J8I47_000633"
     CDS             complement(10646..11293)
                     /locus_tag="J8I47_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322092.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="J8I47_000634"
     CDS             complement(11472..12191)
                     /locus_tag="J8I47_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ6322093.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="J8I47_000635"
     CDS             complement(12322..13473)
                     /locus_tag="J8I47_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAZ6322094.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="J8I47_000636"
     CDS             complement(13690..14430)
                     /locus_tag="J8I47_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6322095.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="J8I47_000637"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="J8I47_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAZ6322096.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="J8I47_000638"
     CDS             complement(14814..15407)
                     /locus_tag="J8I47_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6322097.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="J8I47_000639"
     CDS             complement(15391..16038)
                     /locus_tag="J8I47_000639"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAZ6322098.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="J8I47_000640"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="J8I47_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAZ6322099.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="J8I47_000641"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="J8I47_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAZ6322100.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="J8I47_000642"
     CDS             complement(17484..18011)
                     /locus_tag="J8I47_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAZ6322101.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="J8I47_000643"
     CDS             complement(18191..18976)
                     /locus_tag="J8I47_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAZ6322102.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="J8I47_000644"
     CDS             complement(18969..19838)
                     /locus_tag="J8I47_000644"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAZ6322103.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="J8I47_000645"
     CDS             complement(19839..21218)
                     /locus_tag="J8I47_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAZ6322104.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="J8I47_000646"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="J8I47_000646"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAZ6322105.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="J8I47_000647"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="J8I47_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAZ6322106.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="J8I47_000648"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="J8I47_000648"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAZ6322107.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="J8I47_000649"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="J8I47_000649"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAZ6322108.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="J8I47_000650"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="J8I47_000650"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAZ6322109.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="J8I47_000651"
     CDS             complement(25176..25400)
                     /locus_tag="J8I47_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAZ6322110.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="J8I47_000652"
     CDS             complement(25453..26415)
                     /locus_tag="J8I47_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAZ6322111.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="J8I47_000653"
     CDS             complement(26425..26865)
                     /locus_tag="J8I47_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6322112.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="J8I47_000654"
     CDS             27171..28136
                     /locus_tag="J8I47_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAZ6322113.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="J8I47_000655"
     CDS             complement(28193..28423)
                     /locus_tag="J8I47_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAZ6322114.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="J8I47_000656"
     CDS             complement(28509..29396)
                     /locus_tag="J8I47_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAZ6322115.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="J8I47_000657"
     CDS             complement(29485..30708)
                     /locus_tag="J8I47_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ6322116.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="J8I47_000658"
     CDS             complement(30725..31804)
                     /locus_tag="J8I47_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ6322117.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10589 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt t
//
LOCUS       DADOZM010000016        31737 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.016, whole genome shotgun sequence.
ACCESSION   DADOZM010000016 DADOZM010000000
VERSION     DADOZM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.016"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(24..221)
                     /locus_tag="J8I47_000659"
     CDS             complement(24..221)
                     /locus_tag="J8I47_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAZ6322118.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(615..2342)
                     /locus_tag="J8I47_000660"
     CDS             complement(615..2342)
                     /locus_tag="J8I47_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ6322119.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2335..3528)
                     /locus_tag="J8I47_000661"
     CDS             complement(2335..3528)
                     /locus_tag="J8I47_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ6322120.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3715..4359)
                     /locus_tag="J8I47_000662"
     CDS             complement(3715..4359)
                     /locus_tag="J8I47_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6322121.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4453..4587)
                     /locus_tag="J8I47_000663"
     CDS             complement(4453..4587)
                     /locus_tag="J8I47_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAZ6322122.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4589..6721)
                     /gene="feoB"
                     /locus_tag="J8I47_000664"
     CDS             complement(4589..6721)
                     /gene="feoB"
                     /locus_tag="J8I47_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAZ6322123.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6718..7191)
                     /locus_tag="J8I47_000665"
     CDS             complement(6718..7191)
                     /locus_tag="J8I47_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAZ6322124.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7592..7816
                     /gene="nrdH"
                     /locus_tag="J8I47_000666"
     CDS             7592..7816
                     /gene="nrdH"
                     /locus_tag="J8I47_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAZ6322125.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7975..10134
                     /gene="nrdE"
                     /locus_tag="J8I47_000667"
     CDS             7975..10134
                     /gene="nrdE"
                     /locus_tag="J8I47_000667"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAZ6322126.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10184..11149
                     /gene="nrdF"
                     /locus_tag="J8I47_000668"
     CDS             10184..11149
                     /gene="nrdF"
                     /locus_tag="J8I47_000668"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAZ6322127.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11238..12377)
                     /locus_tag="J8I47_000669"
     CDS             complement(11238..12377)
                     /locus_tag="J8I47_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ6322128.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12686..13399)
                     /locus_tag="J8I47_000670"
     CDS             complement(12686..13399)
                     /locus_tag="J8I47_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAZ6322129.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13653..14354)
                     /gene="nagB"
                     /locus_tag="J8I47_000671"
     CDS             complement(13653..14354)
                     /gene="nagB"
                     /locus_tag="J8I47_000671"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAZ6322130.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14588..16120
                     /locus_tag="J8I47_000672"
     CDS             14588..16120
                     /locus_tag="J8I47_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ6322131.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16311..17513)
                     /locus_tag="J8I47_000673"
     CDS             complement(16311..17513)
                     /locus_tag="J8I47_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322132.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17595..18284)
                     /locus_tag="J8I47_000674"
     CDS             complement(17595..18284)
                     /locus_tag="J8I47_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ6322133.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18390..18758)
                     /gene="rplL"
                     /locus_tag="J8I47_000675"
     CDS             complement(18390..18758)
                     /gene="rplL"
                     /locus_tag="J8I47_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAZ6322134.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18811..19314)
                     /locus_tag="J8I47_000676"
     CDS             complement(18811..19314)
                     /locus_tag="J8I47_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAZ6322135.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19724..20413)
                     /gene="rplA"
                     /locus_tag="J8I47_000677"
     CDS             complement(19724..20413)
                     /gene="rplA"
                     /locus_tag="J8I47_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAZ6322136.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20517..20939)
                     /gene="rplK"
                     /locus_tag="J8I47_000678"
     CDS             complement(20517..20939)
                     /gene="rplK"
                     /locus_tag="J8I47_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAZ6322137.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21282..21950
                     /gene="sdaAB"
                     /locus_tag="J8I47_000679"
     CDS             21282..21950
                     /gene="sdaAB"
                     /locus_tag="J8I47_000679"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAZ6322138.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21963..22835
                     /gene="sdaAA"
                     /locus_tag="J8I47_000680"
     CDS             21963..22835
                     /gene="sdaAA"
                     /locus_tag="J8I47_000680"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAZ6322139.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22926..23945)
                     /locus_tag="J8I47_000681"
     CDS             complement(22926..23945)
                     /locus_tag="J8I47_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAZ6322140.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24118..25005)
                     /locus_tag="J8I47_000682"
     CDS             complement(24118..25005)
                     /locus_tag="J8I47_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ6322141.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25082..25627)
                     /gene="nusG"
                     /locus_tag="J8I47_000683"
     CDS             complement(25082..25627)
                     /gene="nusG"
                     /locus_tag="J8I47_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAZ6322142.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25728..25898)
                     /gene="secE"
                     /locus_tag="J8I47_000684"
     CDS             complement(25728..25898)
                     /gene="secE"
                     /locus_tag="J8I47_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAZ6322143.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25922..26074)
                     /gene="rpmG"
                     /locus_tag="J8I47_000685"
     CDS             complement(25922..26074)
                     /gene="rpmG"
                     /locus_tag="J8I47_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ6322144.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26236..26880
                     /locus_tag="J8I47_000686"
     CDS             26236..26880
                     /locus_tag="J8I47_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ6322145.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26936..27439)
                     /locus_tag="J8I47_000687"
     CDS             complement(26936..27439)
                     /locus_tag="J8I47_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAZ6322146.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27452..27498)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28036..29001
                     /locus_tag="J8I47_000688"
     CDS             28036..29001
                     /locus_tag="J8I47_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAZ6322147.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29100..30272)
                     /locus_tag="J8I47_000689"
     CDS             complement(29100..30272)
                     /locus_tag="J8I47_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322148.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30259..30384)
                     /locus_tag="J8I47_000690"
                     /pseudo
     CDS             complement(30259..30384)
                     /locus_tag="J8I47_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30492..31571
                     /locus_tag="J8I47_000691"
     CDS             30492..31571
                     /locus_tag="J8I47_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6322149.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9672 a         6568 c         5715 g         9782 t
ORIGIN      
        1 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
       61 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      121 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      181 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      241 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      301 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      361 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      421 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      481 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      541 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      601 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      661 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      721 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
      781 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
      841 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
      901 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
      961 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1021 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1081 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1141 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1201 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1261 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1321 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1381 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1441 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1501 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1561 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1621 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1681 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1741 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     1801 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     1861 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     1921 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     1981 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2041 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2101 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2161 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2221 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2281 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2341 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2401 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2461 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2521 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2581 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2641 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2701 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     2761 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     2821 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     2881 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     2941 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3001 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3061 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3121 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3181 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3241 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3301 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3361 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3421 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3481 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3541 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3601 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3661 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3721 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     3781 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     3841 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     3901 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     3961 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4021 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4081 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4141 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4201 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4261 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4321 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4381 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4441 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4501 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4561 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4621 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4681 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4741 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     4801 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     4861 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     4921 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     4981 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5041 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5101 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5161 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5221 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5281 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5341 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5401 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5461 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5521 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5581 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5641 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5701 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     5761 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     5821 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     5881 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     5941 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6001 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6061 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6121 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6181 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6241 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6301 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6361 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6421 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6481 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6541 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6601 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6661 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6721 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     6781 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     6841 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     6901 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     6961 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7021 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7081 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7141 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7201 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7261 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7321 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7381 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7441 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7501 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7561 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7621 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7681 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7741 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     7801 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     7861 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     7921 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     7981 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8041 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8101 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     8161 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8221 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8281 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8341 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8401 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8461 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8521 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8581 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8641 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8701 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     8761 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     8821 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     8881 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     8941 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9001 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9061 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9121 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9181 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9241 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9301 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9361 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9421 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9481 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9541 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9601 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9661 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9721 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
     9781 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
     9841 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
     9901 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
     9961 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10021 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10081 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10141 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10201 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10261 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10321 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10381 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10441 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10501 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10561 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10621 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10681 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10741 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    10801 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    10861 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    10921 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    10981 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11041 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11101 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11161 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11221 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11281 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11341 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11401 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11461 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11521 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11581 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11641 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11701 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    11761 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    11821 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    11881 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    11941 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12001 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12061 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12121 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12181 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12241 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12301 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12361 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12421 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12481 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12541 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12601 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12661 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12721 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    12781 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    12841 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    12901 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    12961 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13021 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13081 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13141 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13201 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13261 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13321 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13381 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13441 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13501 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13561 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13621 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13681 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13741 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    13801 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    13861 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    13921 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    13981 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14041 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14101 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14161 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14221 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14281 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14341 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14401 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14461 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14521 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14581 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14641 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14701 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    14761 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    14821 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    14881 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    14941 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15001 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15061 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15121 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15181 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15241 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15301 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15361 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15421 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15481 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15541 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15601 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15661 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15721 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    15781 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    15841 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    15901 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    15961 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16021 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16081 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16141 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16201 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16261 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16321 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16381 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16441 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    16501 ttgcttgctg cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga
    16561 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16621 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16681 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16741 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    16801 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    16861 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    16921 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    16981 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    17041 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    17101 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    17161 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    17221 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    17281 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    17341 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    17401 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    17461 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    17521 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    17581 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17641 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17701 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    17761 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    17821 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    17881 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    17941 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    18001 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    18061 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    18121 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    18181 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    18241 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    18301 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18361 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    18421 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    18481 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    18541 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18601 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18661 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18721 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    18781 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    18841 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    18901 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    18961 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    19021 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    19081 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    19141 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    19201 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    19261 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    19321 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19381 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    19441 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    19501 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    19561 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19621 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19681 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19741 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    19801 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    19861 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    19921 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    19981 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    20041 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    20101 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    20161 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    20221 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    20281 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    20341 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    20401 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    20461 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    20521 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    20581 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20641 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20701 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    20761 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    20821 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    20881 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    20941 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    21001 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    21061 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    21121 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    21181 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    21241 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    21301 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21361 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    21421 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    21481 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    21541 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21601 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21661 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21721 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    21781 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    21841 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    21901 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    21961 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    22021 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    22081 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    22141 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    22201 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    22261 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    22321 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22381 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    22441 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    22501 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    22561 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22621 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22681 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22741 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    22801 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    22861 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    22921 ttttattaat ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt
    22981 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    23041 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    23101 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    23161 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    23221 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    23281 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    23341 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    23401 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    23461 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    23521 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    23581 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23641 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23701 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    23761 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    23821 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    23881 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    23941 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    24001 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    24061 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    24121 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    24181 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    24241 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    24301 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24361 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    24421 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    24481 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    24541 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24601 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24661 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24721 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    24781 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    24841 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    24901 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    24961 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    25021 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    25081 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    25141 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    25201 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    25261 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    25321 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25381 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    25441 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    25501 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    25561 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25621 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25681 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25741 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    25801 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    25861 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    25921 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    25981 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    26041 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    26101 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    26161 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    26221 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    26281 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    26341 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    26401 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    26461 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    26521 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    26581 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26641 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26701 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    26761 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    26821 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    26881 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    26941 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    27001 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    27061 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    27121 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    27181 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    27241 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    27301 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27361 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    27421 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    27481 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    27541 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27601 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27661 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27721 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    27781 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    27841 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    27901 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    27961 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    28021 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    28081 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    28141 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    28201 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    28261 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    28321 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    28381 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    28441 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    28501 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    28561 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    28621 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    28681 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    28741 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    28801 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    28861 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    28921 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    28981 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    29041 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    29101 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    29161 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    29221 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    29281 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    29341 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    29401 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    29461 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    29521 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    29581 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    29641 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    29701 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    29761 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    29821 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    29881 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    29941 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    30001 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    30061 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    30121 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    30181 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    30241 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    30301 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    30361 caaatcagtg tagaaataaa gtttaggcgg attttataat gaacttagcc atttaaaata
    30421 aaaaaaacgc cacgtgaatt ccatgttata ttgaagttac cacaactctc aatagacaag
    30481 gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga aaacatcttt
    30541 cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc aaccgtcagg
    30601 ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat cgcggaacca
    30661 tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac tattctgttt
    30721 atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt ggcagacgcc
    30781 ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg ttaaatgaaa
    30841 aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc gattgtgcca
    30901 tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg cgaacaaaga
    30961 acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca ggacaccgtc
    31021 ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt gaaacacggg
    31081 aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag tcggatgccg
    31141 tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag ttgaacggga
    31201 aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct ggcgctaact
    31261 tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct gggctacacg
    31321 atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct tgggagcgtg
    31381 ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga agcccgatta
    31441 gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat tacccgcgga
    31501 aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag caagaacggt
    31561 tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg cttcatgtct
    31621 ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt cattatcaag
    31681 gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt tacctgt
//
LOCUS       DADOZM010000017        31648 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.017, whole genome shotgun sequence.
ACCESSION   DADOZM010000017 DADOZM010000000
VERSION     DADOZM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.017"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(57..974)
                     /locus_tag="J8I47_000692"
     CDS             complement(57..974)
                     /locus_tag="J8I47_000692"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAZ6322150.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            1176..1613
                     /gene="zapA"
                     /locus_tag="J8I47_000693"
     CDS             1176..1613
                     /gene="zapA"
                     /locus_tag="J8I47_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAZ6322151.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            1671..2218
                     /locus_tag="J8I47_000694"
                     /pseudo
     CDS             1671..2218
                     /locus_tag="J8I47_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            2333..4693
                     /locus_tag="J8I47_000695"
     CDS             2333..4693
                     /locus_tag="J8I47_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAZ6322152.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            4780..5094
                     /gene="trxA"
                     /locus_tag="J8I47_000696"
     CDS             4780..5094
                     /gene="trxA"
                     /locus_tag="J8I47_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAZ6322153.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            5276..7054
                     /gene="uvrC"
                     /locus_tag="J8I47_000697"
     CDS             5276..7054
                     /gene="uvrC"
                     /locus_tag="J8I47_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAZ6322154.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            7199..8098
                     /locus_tag="J8I47_000698"
     CDS             7199..8098
                     /locus_tag="J8I47_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322155.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            8091..9353
                     /locus_tag="J8I47_000699"
     CDS             8091..9353
                     /locus_tag="J8I47_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322156.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            9516..11414
                     /locus_tag="J8I47_000700"
     CDS             9516..11414
                     /locus_tag="J8I47_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAZ6322157.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(11713..12432)
                     /locus_tag="J8I47_000701"
     CDS             complement(11713..12432)
                     /locus_tag="J8I47_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAZ6322158.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            12769..13101
                     /locus_tag="J8I47_000702"
     CDS             12769..13101
                     /locus_tag="J8I47_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322159.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            13088..15091
                     /locus_tag="J8I47_000703"
     CDS             13088..15091
                     /locus_tag="J8I47_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAZ6322160.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            15110..15580
                     /locus_tag="J8I47_000704"
     CDS             15110..15580
                     /locus_tag="J8I47_000704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAZ6322161.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            15601..16185
                     /locus_tag="J8I47_000705"
     CDS             15601..16185
                     /locus_tag="J8I47_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAZ6322162.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            16200..17186
                     /locus_tag="J8I47_000706"
     CDS             16200..17186
                     /locus_tag="J8I47_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAZ6322163.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            17176..17487
                     /locus_tag="J8I47_000707"
     CDS             17176..17487
                     /locus_tag="J8I47_000707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAZ6322164.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            17509..19290
                     /locus_tag="J8I47_000708"
     CDS             17509..19290
                     /locus_tag="J8I47_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAZ6322165.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            19283..20659
                     /locus_tag="J8I47_000709"
     CDS             19283..20659
                     /locus_tag="J8I47_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAZ6322166.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            20666..21301
                     /locus_tag="J8I47_000710"
     CDS             20666..21301
                     /locus_tag="J8I47_000710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAZ6322167.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            21331..22686
                     /locus_tag="J8I47_000711"
     CDS             21331..22686
                     /locus_tag="J8I47_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAZ6322168.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(22878..24272)
                     /locus_tag="J8I47_000712"
     CDS             complement(22878..24272)
                     /locus_tag="J8I47_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAZ6322169.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            24364..25335
                     /locus_tag="J8I47_000713"
     CDS             24364..25335
                     /locus_tag="J8I47_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAZ6322170.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            25332..26114
                     /locus_tag="J8I47_000714"
     CDS             25332..26114
                     /locus_tag="J8I47_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAZ6322171.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(26283..26654)
                     /locus_tag="J8I47_000715"
     CDS             complement(26283..26654)
                     /locus_tag="J8I47_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAZ6322172.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            26788..27231
                     /locus_tag="J8I47_000716"
     CDS             26788..27231
                     /locus_tag="J8I47_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAZ6322173.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(27274..28083)
                     /gene="yidA"
                     /locus_tag="J8I47_000717"
     CDS             complement(27274..28083)
                     /gene="yidA"
                     /locus_tag="J8I47_000717"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAZ6322174.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(28102..29469)
                     /locus_tag="J8I47_000718"
     CDS             complement(28102..29469)
                     /locus_tag="J8I47_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6322175.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(29453..30283)
                     /locus_tag="J8I47_000719"
     CDS             complement(29453..30283)
                     /locus_tag="J8I47_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAZ6322176.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            30446..30883
                     /locus_tag="J8I47_000720"
     CDS             30446..30883
                     /locus_tag="J8I47_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAZ6322177.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            30959..31579
                     /locus_tag="J8I47_000721"
     CDS             30959..31579
                     /locus_tag="J8I47_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAZ6322178.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        10269 a         5434 c         6817 g         9128 t
ORIGIN      
        1 gagaacaccg tttattcgga acaagggact gtgacatgac ttttgtcaca gttccattat
       61 ttttttagca gcttcaaagc tttttctgta ttggcaaaat gaagtttggc atatttttca
      121 agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga tttcgttcca
      181 gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc ttccaaaaag
      241 ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc tcctttagta
      301 acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt tttaaaattt
      361 gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc gatttttttt
      421 agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc attgtatttt
      481 ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg gatggttttt
      541 tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga atctctaaca
      601 ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca tactgttagc
      661 ggaccaaaat agctgccatt tccgacctca tcactaccta aaacggataa ttgactgata
      721 ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg tgcctccccc
      781 catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc ggatgtataa
      841 gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat ctgtttattt
      901 aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt tgctttgata
      961 atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg caactcacga
     1021 cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat catataatag
     1081 ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa
     1141 ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca
     1201 aagcagtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag catcatatgg
     1261 atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta tcaccacaaa
     1321 tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat caattgaaga
     1381 aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa aaaatgatca
     1441 aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg
     1501 aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg gaagcacaac
     1561 agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat taagatcctt
     1621 ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt
     1681 tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt
     1741 tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag catttttaca
     1801 aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc ccaacatcaa
     1861 agttagcatt ttttgatcag gtatttgctt ttcacctaga tgaagctttt tatgcaggaa
     1921 ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg ggggtttttg
     1981 tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca ggaggcatcc
     2041 tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt gcctttgttc
     2101 cattagtgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta tcgttgagag
     2161 aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt tgaattaagg
     2221 aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa gaaggctgcg
     2281 tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata aaatgaataa
     2341 ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac aatttgtagt
     2401 gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga aacaaacgat
     2461 cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc gaggcgggat
     2521 ccctatacct aaacttgaaa atatccgtcc ccatatgaaa cggatcgaga tcggagcaga
     2581 cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa gcgaattgaa
     2641 tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt atatgtgggc
     2701 agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga
     2761 cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga acattcggcg
     2821 aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta
     2881 tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga
     2941 ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggac agactttatt
     3001 tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc aaatcgctga
     3061 acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc cccatcgtca
     3121 agaaatcatc cacaatgctt atgtgattgg taaaatggat ctgatgaatg caaaagcacg
     3181 atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt
     3241 gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg acatcacaat
     3301 cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga aaacgatcac
     3361 gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga ttcctgcagg
     3421 tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag acgaacagtc
     3481 gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag atattttatc
     3541 aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa cagatccgca
     3601 agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt
     3661 gatggcaaca acgcattatc ctgaattgaa agtttatgga tacaatcggg caaatacgat
     3721 taatgccagc atggagttcg atgtggatac actaagtcca acgtatcgtt tgctgattgg
     3781 cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg atacagaagt
     3841 tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg aaatgatcac
     3901 agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc gtcattttgt
     3961 ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt tctttgaaga
     4021 acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaac gaagtcgtgt cagaggcgga
     4081 agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg
     4141 aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga
     4201 agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc
     4261 gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg
     4321 gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag acgagctgac
     4381 gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac gatctgaatc
     4441 tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag aagcgctaag
     4501 tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag tgactattgt
     4561 ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta aaaaccatcg
     4621 gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt
     4681 taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag tatagaaatg
     4741 ctatactaat catagatttt aaaaatggag gtctttgata tgtcacaagt aattacagat
     4801 aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg ggcaacttgg
     4861 tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga atatgatgaa
     4921 gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc tcaacaattc
     4981 ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt agaaaaagct
     5041 gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt ataaaaactt
     5101 acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt tttttgcggt
     5161 tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca ggctggattg
     5221 tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt accagatgaa
     5281 cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt acttgatgaa
     5341 agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg
     5401 ctcatatttt actggcagcc ataatacgaa aacggaacgg cttgttagtg aaatcgaaga
     5461 ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga tcaatctgat
     5521 aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct atcctttcct
     5581 taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaagtac taaaagacaa
     5641 agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa agaagatcct
     5701 cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc cttgtcttta
     5761 ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac gtagatccga aagtctatac
     5821 gaaaatcgtt gatgaagtga aaaagttttt aaacggagga cacgaaaaaa tcgagtcaga
     5881 gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag ctgcagaata
     5941 ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga caaatactga
     6001 tttactggat cgagatgtat ttggttatgc gattgacaaa ggatggatgt gtgtccaagt
     6061 gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc ctttctataa
     6121 cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa atgagcattt
     6181 cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag aagctttgtt
     6241 agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg aattggcgaa
     6301 taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa aacaggaacg
     6361 gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc caatacgtat
     6421 cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga tggtcgtatt
     6481 cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg
     6541 accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcac gagtgattcg
     6601 cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac aagtagatgt
     6661 ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg gcatggcaaa
     6721 aaacgataaa cataaaacaa atgagttgct attcggtata gatttggctg tagtaccgct
     6781 tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag tccatcgatt
     6841 cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc gattagatga
     6901 aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa
     6961 aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga aaaatgtcgc
     7021 acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac aaagaaagta
     7081 aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg gtaaataata
     7141 aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga gtgaagaaat
     7201 gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg atcatgtctc
     7261 tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg ctgggaagac
     7321 aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag tgttatggaa
     7381 tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg aggaacgggg
     7441 actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac ttagagggaa
     7501 atcaagaaaa gagattgaac caaaaatcga tttttggatg gaaaaatttc aggtaaaagg
     7561 gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag tccagctgat
     7621 cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca gcgggttaga
     7681 tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac atggttcatg
     7741 tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc atttgatcat
     7801 gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg aatcatttgg
     7861 ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag aaatcgaagg
     7921 cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag aagatcctga
     7981 agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta tgtttaacca
     8041 gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg atgcgtaagt
     8101 tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct ttcttgatca
     8161 tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac tttgctcaac
     8221 aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc agtcagcaaa
     8281 tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa gaaaaagcac
     8341 aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact gagaatgata
     8401 agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa ctactgatac
     8461 aacaacaact gactggcttg caatcgatgc ttcgagcaag tcaattaggc atttcaccag
     8521 aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc aattttgatt
     8581 caaaaggaaa aatgacagtt ggagaagata actcgacagt ccaatatgtc gtaagttatg
     8641 tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt gcacaagaga
     8701 tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg actgcacaaa
     8761 agcattttta cggaaaacta acaggtgttt tacttgttgc attaacgcaa atggcattat
     8821 acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg gtcaaaaata
     8881 tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat tctcttctat
     8941 tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc tctttggtca
     9001 acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct ctaggcggat
     9061 atatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt cggatcactt
     9121 cgtatattcc attcttatct tcatatatta tgccggttcg cttagcaaat gagacagtcg
     9181 gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc ttattgatga
     9241 tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta tagcgaaggc ggattatgga
     9301 gttcattgaa acaatcgatt tcaattatga gaaatgaacg aaaaaaagca taaaatatag
     9361 aaaaatatag aaggaagctg gacatttatt gttcccagct tcttttttgt atagacctat
     9421 ttctattttt ttattatatt ggatatatat attcattgaa aatagagcat aacaacttta
     9481 taatgttgag tataccaatt tttggaggcg ttttaatgga ccgctattta gatttacttt
     9541 cagagaagtt ccctacaacg gaagatgtca tcacggaaat catcaatctc gaagcaattt
     9601 tgcagcttcc aaaaggaaca gagttatttt tgagcgatat acatggcgaa tttcctgctt
     9661 ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa gaccttttct
     9721 ctgaacaaat gacggaaacg gagatcagcc aattcactat ttttacggct tatccagaat
     9781 atgtgttgac gactgattgg tacaaaaaac aagataaatc ccagctgatc catcgtttga
     9841 tcgatctttt acggtttacg acagtcaaat acactcgatc aaaagttcga aaaggattgc
     9901 cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgatcgt atcaacggga
     9961 aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt gatgaaggaa gaagaaaaat
    10021 ttttaacaaa acttgcgcag acgatccaaa aatctgtcat cgatcatttg catatcgtgg
    10081 gtgatatttt tgatcgagga actcaagcag ccaaagtaat ggatcgaatc atggattttt
    10141 cctctgtaga tatccagtgg gggaatcatg atattttgtg gattggtgcg tattgcggct
    10201 ctgaggcatg tttattgacg cttttacgga ttgccgcacg ctacaactat ttatttgaat
    10261 tagagaaaga atatggatta aatttacgtc cattattttc ttttgcccat caaacctatc
    10321 agaagaatcc agtatttacc cctaaaaaca ggaaagatct atctgaaaga gagcaagaag
    10381 aattagaaaa gatccatcaa gcactttcta tcctccaatt caaatcagaa catcagttgt
    10441 tgaatcgacg acctgaattt aaaatggacg atcgtaaatt gttggaaaaa atcaactatg
    10501 aggaatcaac gatcgtttta gaaggtcagc tctatgagtt gaaagggact tgttttcaaa
    10561 cgatccagcc agatgatcct aaaaaattct tggaagagga acaaaaagtg atcgatagtt
    10621 tgatgtattc attccagcac tcgttaagaa tgcaaaaaca catgacgttt ttgatcgaaa
    10681 aagggggaat gtatctcacg aacaatcagc atgtactgtt ccatggctgt atacctgtag
    10741 atgaaaaggg gcagctctta gcatttgaat tagaccaatc ttatcgcgga aaagaactgc
    10801 tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga tttgactgca aatgaagatt
    10861 tggcgaccga tttgatttgg tatgcgtgga gcgggccatt ttcgccttta ttcggcaaga
    10921 gtaaaatggc aacttttgag cgctattttc tcacagagcc tcatacgcac gtcgaaaaaa
    10981 gcaatcccta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt ttggcagaat
    11041 tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca cacgccagta aaagtaaaaa
    11101 aaggagagtc accgatcaaa gcaaacggaa aagcatttgt tattgacgga ggtctatcca
    11161 aagcttatca aaaaacaacc gggattgctg gctactcatt actatgcaat tcttacggtt
    11221 tccaaatcgt gacacattat ccgttcttgc ctattgaaca acaatttgca aaaaaatctg
    11281 atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc acgcaaactt aatcggaata
    11341 cgacaaaagg aatggaatta cagcaacaga ttactcaact ccaaaggctg ttggattatc
    11401 gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa tagactaaaa agcgactgca
    11461 acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa aaacggtaaa atattagaag
    11521 ctatttttct gtttatttct ctaatactca ggtcagggcg ctgatttcac gacctcttcc
    11581 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccaa caaacatgag
    11641 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctaa
    11701 tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat aaacgctgtt cagatgaatc
    11761 tacacgatga gaaagttccg gatgccattg gacaccaagg attttgtgca tattatcttt
    11821 actttggact gcttcaatga gtccatcgtt ggaaaaggcg atggctttta acaaaggaga
    11881 aagatctttg actgcctgat gatgatagga attcacaaag tatgaatcag ggagaaggtc
    11941 acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt gcaaactgcg gggcagtggg
    12001 ctgttgttcg tgttttacgg accattttgg atataatgaa agatcttgat aaagtgttcc
    12061 ctccaacacg acattcaata gttgaagtcc tcggcagact gcaaaaatcg gttttctttg
    12121 tttcaatgct tccaagatca atgcctgttc gaattggtct cgttgaatat ttgtttccgt
    12181 cagttttgga tgagggtctt ccatgtaaag ttgcggacta acatcctgac cgcctgcaag
    12241 aatgagtttg tcgattttct caattagttg aggcgctgaa tctggagaca tgactggcag
    12301 aataagaggt aatccttgag catctaaaac tgcatccaca aatccctgcg gtgtatagct
    12361 tacttgattt ccttggaaag tatctgtcgc tcgaatcagt tgattcccgg caatgccgat
    12421 gattggtttc atttttattc cccctaaagt agactaattg taacaaggat ttaaaaaaat
    12481 ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac tatctgtaaa
    12541 gagaattttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt tgttataata
    12601 aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt cttcaagggg
    12661 tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag aggtacatac
    12721 tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt gaagcaagtg
    12781 ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgagaagcg tgctcgaacc
    12841 ctacggcagc agttgaagac ctatgcagaa gaaaaagagc aagaattgga gataatccaa
    12901 acgaacagtc ggcaacagct gaaacagttg gtagaagaaa aagaacaatt cgaaaagcaa
    12961 gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca agcaactttg
    13021 aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg caatcattga aagggtgaaa
    13081 gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata aaagaaatca
    13141 ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt gaaatccggg atctgtatca
    13201 aaatagaaca aacaaccagt gggtagaaaa atattttcca gataccacga ttattgataa
    13261 agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac atccaagaag ctatccaatt
    13321 catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc aagaactttc
    13381 cttacaagac ttggaagcaa attattccga agaaaatttc atgaataaat tagaagaagt
    13441 ccttgattta aaagaaaagt gggaacagct aactgaaaaa cgtgatcgtt tagaagaaga
    13501 ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt atgcaagtaa
    13561 gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt tcaaggaaga
    13621 actaagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa aacaagttta
    13681 tctttcgtat gttgctttaa aaaaatcagt tgctgctata gaagaaattg ctgggaaata
    13741 cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaat tgcaagaaac
    13801 caaacagcaa ttatccattg ttcacgagca gcaaaaaaag ataacgacag cgttaggtca
    13861 gtgcagcggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg ccgttgcaga
    13921 acgggaaaaa gtaaaagaac ggtttatcca tgccacttat ctgatcgttt tgcaaggttg
    13981 ggtagatgca gaagaaaaac agaatttgat gcatgtgtta aacgaaactg tatcagaaga
    14041 agatatatac gttagtttcg aaacaccaaa ctcagaagag attcagcagg aagtccctac
    14101 taaattgaaa aaccatccgc ttatcgaacc ttttgaattg ctgactgaaa tgtacagtct
    14161 gccaaaatat gaagaagtag atccaacgcc ttggatgaca ccattttatc ttgtcttctt
    14221 cgggatgatg gtcgcagatg ttggttacgg attgctgatg ctcattggga caatactagc
    14281 tcaaaaacta tttgttttac cccgcgggat gaaacgtttc gtaaaattct ttgagattct
    14341 ctcggtacca tcgatcatct ggggattgat ctacagttca tttttcggac aggcattacc
    14401 aaaagaacta tttggtatcc gcttgccttt cccaatccta tctacgacgg atgacgtcaa
    14461 tacgattttg attttatccg tgattttcgg tctgatacag atcttggttg gattattcgt
    14521 ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt gctgtcagtg atggttttgc
    14581 ctggcaaggg atcctgattg ggatcgttat tgcattgatt ggtgtcatgt tgataaaaaa
    14641 tacagcattt gtttatcttg gcgcggggct atcgatcatt gctgctttat gtatcttaat
    14701 cgtgccgatt atccaatcgc cgtctaaagt caagggtgct gccaaaggag cgtataatct
    14761 ttacggattg actggttata tcggtgattt agtcagttat acacgtttga tggcactagg
    14821 tatttcaggc ggaagtattg gcgcggcatt caatatgcta gttgctttca tgccgccagc
    14881 cgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga atatgttttt
    14941 gacactgtta agtgcctatg tccatggtgc acgattgcag tatgtcgaat tttttggaaa
    15001 attttataca ggaggcggac gtgcctttga cccgctgaaa acggcggaaa aatacgtcaa
    15061 catcaatcat aagaaaaaaa acaataaata attggaggat ttttatacaa tgatggatta
    15121 cttaattagt caaaatggtg gaatggtatt tgcagtatta gcgatggcaa cagcgacgat
    15181 tttttcaggg attggttcag ctaaaggtgt tggaatgaca ggtgaggcag cagcagcgtt
    15241 aacaacaagt caacccgaaa aattcggaca ggctttgatt ctgcaattgc ttccaggtac
    15301 gcaagggttg tatggattcg ttatcgcctt tttgattttt atcaacttag gaaatgatat
    15361 gtcagttgta caaggattga actttctagg cgcttctctg ccgattgctt ttacaggttt
    15421 gttttcaggt atcgcccaag ggaaagttgc agcagcaggt atccaaattt tagcgaaaaa
    15481 acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat atgcgatttt
    15541 aggtttcgtt atttccttcc tattagtatt gaatgcctaa gcataaagga ggaaaagcag
    15601 atgaacgcca ttgaaaaaat catctctcag atgaatgaag cagcagagca agaaagagcg
    15661 gcgttagagc aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa acgtacgcaa
    15721 atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattacttga aaaaaaatac
    15781 cgtcagctta ggaatcgcca acaagtagaa gtacgacaag aaaatttgaa tgcgaagcag
    15841 gaatttttac gacgattgtt tgcagatgca atagctgaaa tggagaattg ggatgaatct
    15901 gaacaaatcc agtttatcaa aaattcattg tattcattac ctttgacagg caaagtaaca
    15961 ttcattgctg gagaaaaatc agcagcttat ctttctcaga cattgctgga tgaatggaac
    16021 aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca agcgggtttt
    16081 ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt acaagatatc
    16141 caaggaacga tgagttttga gattgccaat caattattcg aataaggagg gatcagtgta
    16201 tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt ttgtccaatg
    16261 agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa atattgaaat
    16321 ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa cagaatcttt
    16381 taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag cctgaaatcg
    16441 tttggatcta tacgatgcgc tatactttcc ataatctgaa ggttttgaca aaagccgaga
    16501 tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca ttagaaacct
    16561 tgaaggatgc cattcatacg caagtttcaa atgaactccc tgaaagcatt atggacagta
    16621 tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt gacgtgattt
    16681 atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga tatcaagaat
    16741 tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg gctcgaggaa
    16801 tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg ggaagtatcg
    16861 aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg aattttctat
    16921 tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat cttgatctcg
    16981 taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa gcacaaaccc
    17041 aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta gaaagcaaaa
    17101 accttcgttt actggttatc ggcaaaagaa gccatttcga accagaaaca atcagaaagc
    17161 gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga ttctgttttg
    17221 ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac cgttcgtcag
    17281 gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga gcaatgtgca
    17341 gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc tatcgtattg
    17401 attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa aaacgtagaa
    17461 aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt gcaaattgga
    17521 aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga tgccagtatc
    17581 caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga gatgcgtggc
    17641 gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg cgaaccggtt
    17701 cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc acaaatgttt
    17761 gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa tttcttaggg
    17821 cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga accaacagta
    17881 gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga aacaaaagtt
    17941 ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac aatcgaaaaa
    18001 ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg tgacaaaaat
    18061 ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga aaaattaaac
    18121 ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc agtgacaaaa
    18181 ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt tcagcatcaa
    18241 attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga gcgtggaaat
    18301 gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac aggagaatcg
    18361 ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc agctcgagaa
    18421 gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg ttatgacgtt
    18481 gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat gagcggacgt
    18541 ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg gcttgcggaa
    18601 tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga aggaagtata
    18661 accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt cacacaaaat
    18721 acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca aaaaagacat
    18781 tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt agggaagtat
    18841 atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat gcgtattttg
    18901 caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc cctttcagat
    18961 aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt gcagcaaaat
    19021 gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat gttgaagatg
    19081 atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta tttaagtgag
    19141 atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata tatcccggaa
    19201 acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat gcaaacgatc
    19261 gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg aagtcgtcgg
    19321 tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa tcgaagtccg
    19381 tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag ataaagcaat
    19441 ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg tacgattttt
    19501 aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat tcgatggttt
    19561 gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg atattaacgg
    19621 agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa caggtatctc
    19681 tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat tttctggctc
    19741 tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg tactcgattc
    19801 atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag aagctgaatt
    19861 tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt ttatgaattt
    19921 agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga ctgctgcaga
    19981 atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca tgaccaacta
    20041 tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa gaagaggata
    20101 tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa gaatccgcgg
    20161 actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg ataaaactca
    20221 tccaatccct gacttgactg gttatattac agaaggacag atcatcttga ctagagagct
    20281 ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc ggttaaaaga
    20341 taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga atcagctttt
    20401 tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag gtgaatctgc
    20461 tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa aagaatatgt
    20521 caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag ggtgggaact
    20581 gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc tggacaaata
    20641 tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga cccgcatgga
    20701 attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat tattaaaaga
    20761 taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca accagctgcg
    20821 tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg caaatgcttc
    20881 gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg ttgatttgtc
    20941 tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg aatatgatga
    21001 acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg aactggatcg
    21061 gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag aaatcgaaaa
    21121 aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca atgcattaga
    21181 atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga agctggaaga
    21241 aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta ccactgacta
    21301 agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca aataaagaaa
    21361 cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt agttggcggc
    21421 ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt tatcgatgcg
    21481 cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa tacagcagag
    21541 cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg cggtcttggt
    21601 tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag tctcagcaca
    21661 cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat gaagctcatg
    21721 ctttacatcc taaaattggc ctttgttatc caattgattg gtacggttgc tttatctttt
    21781 gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt ccatgcaatc
    21841 tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa tcaccagcaa
    21901 aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt aggcttcatc
    21961 gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca ttcaaaaatc
    22021 gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt tttcacagag
    22081 cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa cacctttttt
    22141 atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca tatgagccat
    22201 gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg gtcgacggcc
    22261 ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc gatgttgaaa
    22321 ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt gttgcgggca
    22381 cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat tttatcggtg
    22441 actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt cttctctgcg
    22501 tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat agggaaattg
    22561 atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt cttctctctg
    22621 atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag tgtcatgatt
    22681 ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa ataaaataat
    22741 ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg aggcactacc
    22801 cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa gatttgattc
    22861 ctttatttat caataggtta taattcatat ttttttattt tttcttttac gatacttagt
    22921 ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa tgcatctggt
    22981 tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc aagaaagtca
    23041 ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata gtaagggaca
    23101 cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc tctttttgta
    23161 agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat ttgagtgatg
    23221 acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg ttctatcttg
    23281 ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca aataaatttt
    23341 gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg aggaatgatc
    23401 gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac ccatagacct
    23461 tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg aaaccctata
    23521 ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct acattcctga
    23581 atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat ctctaaatga
    23641 tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc tattctatca
    23701 cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc ggaaacaata
    23761 gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc tgcagtagtc
    23821 aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag atttgggtta
    23881 ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa agaactaacc
    23941 agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat ggactcaaag
    24001 acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc tttctcaatt
    24061 ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc agctagctct
    24121 ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc ttgttctaat
    24181 tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg tcgccaagtt
    24241 aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa ttgtatacca
    24301 tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag gagggaattg
    24361 tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga agaagcgatt
    24421 caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca aaatcctcga
    24481 ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat gcctgcttat
    24541 ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga taatcatcag
    24601 tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa cggactaccc
    24661 aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt atctgcgctg
    24721 gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg agctggtcaa
    24781 caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac agtgtttgtt
    24841 tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa acagtatcct
    24901 aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc ggaaatcatt
    24961 tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag ggaagacgca
    25021 catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac atcgttgatg
    25081 gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag cggggacctg
    25141 cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac attaggcgaa
    25201 ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat tttcgacgca
    25261 gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa attagagaaa
    25321 ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat gaggtgttca
    25381 aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt acgatgcaaa
    25441 aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa aaccaagaat
    25501 ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac gcgacattag
    25561 caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt aaattttcta
    25621 ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg gattttccta
    25681 gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt ggggctaaga
    25741 ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa aaaacggtgg
    25801 ctaaattgca gccagatttt acagctatca aaaattttga attaggaata ggagtcatcg
    25861 tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca aaattaacaa
    25921 tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat tgggcagaaa
    25981 gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg gatctagaat
    26041 gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt tctaaaggta
    26101 cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga gtggcaacct
    26161 tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg aaaagtttgc
    26221 gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat gccccatact
    26281 aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa gtagtaagaa
    26341 ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccggcagc tttatgttgc
    26401 tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc gtctcctaga
    26461 tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc cccaggaagt
    26521 gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct ttcttcaaaa
    26581 ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg aacacatgtg
    26641 tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa caaaaagaag
    26701 taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa tggatagaaa
    26761 ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga aaaaaagcaa
    26821 tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga tcagcagatt
    26881 gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat tgaaggagaa
    26941 gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat tgtggcagaa
    27001 atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg tgcaacttat
    27061 tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg agaaccaact
    27121 gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa attgaaacgt
    27181 ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg aaagaaagcg
    27241 agtagatcag ctttctctca gtttttttat ttattacagt acatattttt tgattgcttc
    27301 agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt tgacaagaga
    27361 aacggcattt cccattgcta ctcctagacc agcatattca atcatggata agtcgttctc
    27421 gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg cgagattcgc
    27481 aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg gcgcactttt
    27541 gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg catctaaaat
    27601 ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca tttcttcagg
    27661 tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat aagcgctgat
    27721 atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac tgccgacacg
    27781 gcgtgccatc acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac gggcgatgat
    27841 tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt aatcattatc
    27901 ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac ggccagtgca
    27961 taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta cttcttttgt
    28021 tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta gtttgataga
    28081 cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac ggatatggct
    28141 ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg acggatcaac
    28201 catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta atgcagcaac
    28261 aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa tctcgatttg
    28321 tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt agctagaatt
    28381 cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag tgagttcttg
    28441 gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt tacgattcaa
    28501 aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt gagtaaagta
    28561 agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc ttccgtcaag
    28621 aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt gatacaactc
    28681 gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa caggatggaa
    28741 gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg catgcatgcc
    28801 actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata ttcgggtaag
    28861 atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat agtccattcg
    28921 atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt aagttttttg
    28981 gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa tctgatacac
    29041 ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg tatggaagat
    29101 atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg ccgcacataa
    29161 agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt ttgaaaaatt
    29221 tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg aatgagtaaa
    29281 acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt taatgcgtcg
    29341 tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct ggacatggat
    29401 atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat atttataagg
    29461 ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg atttggttgt
    29521 tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt aaatcacttg
    29581 cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt acaaaaaggg
    29641 gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca ggatagccat
    29701 ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact tgcccgcgtt
    29761 tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt ttgatccgac
    29821 gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt ccaggacaat
    29881 aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat ttagtcaata
    29941 ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt tgggggaaaa
    30001 tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct gaatttctga
    30061 tcactggagc gtaacctgca tctttcagtg tttggatccc gtcacttaga aaaggagttc
    30121 gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt aaaaagattt
    30181 gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg gaaaagtcgt
    30241 ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg gctcctttgt
    30301 ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt tggaaaaaag
    30361 gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga acaatgtagc
    30421 tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga tttcaatcaa
    30481 attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg atttgaaagg
    30541 gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc aggaggagac
    30601 gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac agctgatccg
    30661 tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag aaacatccta
    30721 tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc atgttagttt
    30781 gaaagaccgt cccacaggag ggaaggttcg aatcgattat gatctgttca tgggaccgga
    30841 aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa agaaaagttt
    30901 gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg tgtcctgatt
    30961 ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg aagtagcaca
    31021 cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta accagatcca
    31081 aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat tttatactga
    31141 cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct tgcgttcatg
    31201 gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag aggatgaaga
    31261 tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg atgaagacgc
    31321 gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag atgacgatga
    31381 tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt ctgacttgca
    31441 agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa atatcgaaga
    31501 agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg aagaattttc
    31561 tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga gacgatcagc
    31621 ctatcagaat aaggaggtgt gacacgat
//
LOCUS       DADOZM010000018        31487 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.018, whole genome shotgun sequence.
ACCESSION   DADOZM010000018 DADOZM010000000
VERSION     DADOZM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.018"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(76..543)
                     /locus_tag="J8I47_000722"
     CDS             complement(76..543)
                     /locus_tag="J8I47_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAZ6322179.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="J8I47_000723"
     CDS             complement(663..2201)
                     /locus_tag="J8I47_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAZ6322180.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="J8I47_000724"
     CDS             2281..2967
                     /locus_tag="J8I47_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAZ6322181.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="J8I47_000725"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="J8I47_000725"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ6322182.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="J8I47_000726"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="J8I47_000726"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAZ6322183.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="J8I47_000727"
     CDS             complement(4453..5583)
                     /locus_tag="J8I47_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ6322184.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="J8I47_000728"
     CDS             complement(5583..7766)
                     /locus_tag="J8I47_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAZ6322185.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="J8I47_000729"
     CDS             complement(7763..8788)
                     /locus_tag="J8I47_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6322186.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="J8I47_000730"
     CDS             complement(8833..9660)
                     /locus_tag="J8I47_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ6322187.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="J8I47_000731"
     CDS             complement(9657..10724)
                     /locus_tag="J8I47_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAZ6322188.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="J8I47_000732"
     CDS             complement(10750..11586)
                     /locus_tag="J8I47_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322189.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="J8I47_000733"
     CDS             complement(11583..12452)
                     /locus_tag="J8I47_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322190.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="J8I47_000734"
     CDS             complement(12522..13805)
                     /locus_tag="J8I47_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322191.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="J8I47_000735"
     CDS             complement(13819..15504)
                     /locus_tag="J8I47_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAZ6322192.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="J8I47_000736"
     CDS             15779..16804
                     /locus_tag="J8I47_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6322193.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="J8I47_000737"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="J8I47_000737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAZ6322194.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="J8I47_000738"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="J8I47_000738"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAZ6322195.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="J8I47_000739"
     CDS             25476..26444
                     /locus_tag="J8I47_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322196.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="J8I47_000740"
     CDS             26462..27367
                     /locus_tag="J8I47_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322197.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="J8I47_000741"
     CDS             27364..28170
                     /locus_tag="J8I47_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322198.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="J8I47_000742"
     CDS             28327..29295
                     /locus_tag="J8I47_000742"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAZ6322199.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="J8I47_000743"
     CDS             complement(29612..30136)
                     /locus_tag="J8I47_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAZ6322200.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31487
                     /gene="scm"
                     /locus_tag="J8I47_000744"
     CDS             30423..>31487
                     /gene="scm"
                     /locus_tag="J8I47_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAZ6322201.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8835 a         6798 c         5928 g         9926 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DADOZM010000019        29934 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.019, whole genome shotgun sequence.
ACCESSION   DADOZM010000019 DADOZM010000000
VERSION     DADOZM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.019"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(336..1793)
                     /gene="ascB"
                     /locus_tag="J8I47_000745"
     CDS             complement(336..1793)
                     /gene="ascB"
                     /locus_tag="J8I47_000745"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ6322202.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1790..3685)
                     /locus_tag="J8I47_000746"
     CDS             complement(1790..3685)
                     /locus_tag="J8I47_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6322203.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3743..5518)
                     /locus_tag="J8I47_000747"
     CDS             complement(3743..5518)
                     /locus_tag="J8I47_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ6322204.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5568..7064)
                     /locus_tag="J8I47_000748"
     CDS             complement(5568..7064)
                     /locus_tag="J8I47_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6322205.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7258..8880)
                     /locus_tag="J8I47_000749"
     CDS             complement(7258..8880)
                     /locus_tag="J8I47_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAZ6322206.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(9329..10642)
                     /locus_tag="J8I47_000750"
     CDS             complement(9329..10642)
                     /locus_tag="J8I47_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAZ6322207.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10887..11111)
                     /locus_tag="J8I47_000751"
     CDS             complement(10887..11111)
                     /locus_tag="J8I47_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322208.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(11131..11499)
                     /locus_tag="J8I47_000752"
     CDS             complement(11131..11499)
                     /locus_tag="J8I47_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAZ6322209.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11697..12407)
                     /locus_tag="J8I47_000753"
     CDS             complement(11697..12407)
                     /locus_tag="J8I47_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322210.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12425..12922)
                     /locus_tag="J8I47_000754"
     CDS             complement(12425..12922)
                     /locus_tag="J8I47_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6322211.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12959..13411)
                     /locus_tag="J8I47_000755"
     CDS             complement(12959..13411)
                     /locus_tag="J8I47_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6322212.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13546..14151)
                     /locus_tag="J8I47_000756"
     CDS             complement(13546..14151)
                     /locus_tag="J8I47_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAZ6322213.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(14601..15221)
                     /locus_tag="J8I47_000757"
     CDS             complement(14601..15221)
                     /locus_tag="J8I47_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6322214.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(15223..16092)
                     /locus_tag="J8I47_000758"
     CDS             complement(15223..16092)
                     /locus_tag="J8I47_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAZ6322215.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(16115..16522)
                     /locus_tag="J8I47_000759"
     CDS             complement(16115..16522)
                     /locus_tag="J8I47_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAZ6322216.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(16541..17830)
                     /locus_tag="J8I47_000760"
     CDS             complement(16541..17830)
                     /locus_tag="J8I47_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6322217.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(17857..18159)
                     /locus_tag="J8I47_000761"
     CDS             complement(17857..18159)
                     /locus_tag="J8I47_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6322218.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(18184..20277)
                     /locus_tag="J8I47_000762"
     CDS             complement(18184..20277)
                     /locus_tag="J8I47_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ6322219.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(20766..21530)
                     /locus_tag="J8I47_000763"
     CDS             complement(20766..21530)
                     /locus_tag="J8I47_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAZ6322220.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(21641..22510)
                     /gene="mdcB"
                     /locus_tag="J8I47_000764"
     CDS             complement(21641..22510)
                     /gene="mdcB"
                     /locus_tag="J8I47_000764"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAZ6322221.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(22535..23611)
                     /locus_tag="J8I47_000765"
     CDS             complement(22535..23611)
                     /locus_tag="J8I47_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAZ6322222.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(23635..24228)
                     /locus_tag="J8I47_000766"
     CDS             complement(23635..24228)
                     /locus_tag="J8I47_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAZ6322223.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(24232..25908)
                     /gene="mdcD"
                     /locus_tag="J8I47_000767"
     CDS             complement(24232..25908)
                     /gene="mdcD"
                     /locus_tag="J8I47_000767"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAZ6322224.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(25898..26197)
                     /locus_tag="J8I47_000768"
     CDS             complement(25898..26197)
                     /locus_tag="J8I47_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAZ6322225.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(26299..27945)
                     /gene="mdcA"
                     /locus_tag="J8I47_000769"
     CDS             complement(26299..27945)
                     /gene="mdcA"
                     /locus_tag="J8I47_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAZ6322226.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(27952..28863)
                     /locus_tag="J8I47_000770"
     CDS             complement(27952..28863)
                     /locus_tag="J8I47_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAZ6322227.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            28983..29840
                     /locus_tag="J8I47_000771"
     CDS             28983..29840
                     /locus_tag="J8I47_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6322228.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         8601 a         6348 c         5060 g         9925 t
ORIGIN      
        1 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
       61 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
      121 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
      181 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
      241 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
      301 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
      361 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
      421 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
      481 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
      541 gcttccttca tctgctcaat atgctgtttc aaatagtcaa tgcggtaatc gtcttgaacg
      601 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
      661 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
      721 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
      781 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
      841 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
      901 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
      961 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
     1021 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
     1081 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
     1141 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
     1201 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
     1261 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
     1321 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
     1381 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
     1441 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
     1501 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
     1561 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
     1621 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
     1681 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
     1741 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
     1801 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
     1861 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
     1921 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
     1981 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
     2041 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
     2101 cttccccgtt tacaggactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
     2161 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
     2221 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
     2281 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
     2341 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
     2401 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
     2461 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
     2521 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
     2581 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
     2641 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
     2701 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
     2761 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
     2821 gacctacaac taataaagta attgggacca tcacgatgat acaaatgaat ggtgtcaata
     2881 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
     2941 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
     3001 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
     3061 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
     3121 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
     3181 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
     3241 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
     3301 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
     3361 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
     3421 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
     3481 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
     3541 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
     3601 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
     3661 agttcttaga aaacggtttc tccattcaat tagtacccct tataatagcg aacgaaactg
     3721 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
     3781 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
     3841 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
     3901 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
     3961 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
     4021 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
     4081 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
     4141 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
     4201 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
     4261 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
     4321 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
     4381 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
     4441 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
     4501 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
     4561 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
     4621 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
     4681 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
     4741 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
     4801 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
     4861 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
     4921 tcggcatgat attggaaaaa attgtactga tttgttgagg caaccttgcg tgttggatca
     4981 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
     5041 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
     5101 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
     5161 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
     5221 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
     5281 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
     5341 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
     5401 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
     5461 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
     5521 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
     5581 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
     5641 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
     5701 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
     5761 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
     5821 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
     5881 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
     5941 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
     6001 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
     6061 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
     6121 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
     6181 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
     6241 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
     6301 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
     6361 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
     6421 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
     6481 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
     6541 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
     6601 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
     6661 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
     6721 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
     6781 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
     6841 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
     6901 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
     6961 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
     7021 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
     7081 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
     7141 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
     7201 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
     7261 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
     7321 tttcagagga taatttgaca caataaccct taaagcttca cgtggaacag tgaacgtctg
     7381 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
     7441 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
     7501 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
     7561 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
     7621 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
     7681 tcctttcgca ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
     7741 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
     7801 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
     7861 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
     7921 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
     7981 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
     8041 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
     8101 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
     8161 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
     8221 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
     8281 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
     8341 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
     8401 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
     8461 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
     8521 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
     8581 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
     8641 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
     8701 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
     8761 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
     8821 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
     8881 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
     8941 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
     9001 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
     9061 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
     9121 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc gtttttttct ctaatactca
     9181 ggccagaaca ctgatttcac aacctcttta taaacggtgt tcactcagct gtccctcggt
     9241 attagctcaa aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc
     9301 aggccagaac gctgatttca caacctcttt atttcacatt caatatccac atgaatccca
     9361 caaatacaaa gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta
     9421 acatggtaat tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca
     9481 aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga
     9541 tttgtcgcaa agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt
     9601 gtgaagtaac aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg
     9661 tagtaatagc tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa
     9721 atgcaccaac aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag
     9781 ctaaagcttt ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta
     9841 atccaactaa agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga
     9901 cccacatttg aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt
     9961 tcaaacttgg tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac
    10021 ctaaaatcgt tgtagctgct atcccgatga agatgccacc tggtacacgc cgaacaagca
    10081 agatagcagt tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc
    10141 ctaatcctac taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga
    10201 aagcgataaa tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat
    10261 caatgatcat ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga
    10321 atacgccggc taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa
    10381 aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca
    10441 ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca
    10501 ttccagaagc acctaataca gttggattta caaataagat ataagccatt gagataaatg
    10561 ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt
    10621 aggaaagtat tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc
    10681 cgttaaaatt tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat
    10741 aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct
    10801 ttatctcaaa gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata
    10861 aagtttcttc attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc
    10921 tgttcttctt tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa
    10981 cctgtatcga ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt
    11041 gctcgaagct tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc
    11101 attagatcca ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac
    11161 aaaataattg ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat
    11221 ttctcctggt tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa
    11281 agaaggcggt ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac
    11341 gcctaattct aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc
    11401 gattgtttca gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac
    11461 gtcattgaca taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt
    11521 tgattttctt tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt
    11581 gtttgatact attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac
    11641 tttttttaca aaaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
    11701 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
    11761 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
    11821 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat tgttctttgg
    11881 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
    11941 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
    12001 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
    12061 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
    12121 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
    12181 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
    12241 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
    12301 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
    12361 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
    12421 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
    12481 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
    12541 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
    12601 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
    12661 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
    12721 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
    12781 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
    12841 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
    12901 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
    12961 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
    13021 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
    13081 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
    13141 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
    13201 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
    13261 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
    13321 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
    13381 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
    13441 ctagtttacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
    13501 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
    13561 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
    13621 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
    13681 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
    13741 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
    13801 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
    13861 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
    13921 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
    13981 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
    14041 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
    14101 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
    14161 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
    14221 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
    14281 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
    14341 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
    14401 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
    14461 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
    14521 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
    14581 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
    14641 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
    14701 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
    14761 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
    14821 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgacataa cacttcaaag
    14881 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
    14941 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
    15001 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
    15061 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
    15121 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
    15181 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
    15241 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
    15301 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
    15361 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
    15421 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
    15481 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
    15541 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
    15601 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
    15661 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
    15721 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
    15781 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
    15841 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
    15901 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
    15961 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
    16021 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
    16081 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
    16141 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
    16201 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
    16261 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
    16321 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
    16381 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
    16441 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
    16501 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
    16561 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
    16621 cattgatgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
    16681 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
    16741 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
    16801 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
    16861 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
    16921 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
    16981 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
    17041 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
    17101 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
    17161 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
    17221 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
    17281 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
    17341 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
    17401 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
    17461 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
    17521 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
    17581 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
    17641 aagttaaact tccaacgatg atgctcgaac cggcactcaa cacttgaaat cctaggagtg
    17701 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
    17761 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
    17821 taacgcccat gtttttaccc tcttttcttt ttgtatttag ataactccct tatcttgcag
    17881 aactggggtg attttttctt caatttcatt ttttgaaaca atatttttca aatagatcat
    17941 cgccgtttta ggatcaactg cgaacttttc gaactgcgaa cgaaaatttt ctgctgtgat
    18001 gatcacatct ggtttcatgg aaactgcgga agaaatatca caatggtcca gtttggcttc
    18061 tactcctaat ttgttcaaaa catcctcagc agacatctgt gctgcaaaac tacttcctaa
    18121 acctgcaccg catacgaata aaatgttcaa tttgttcatc tttgttctcc cccgtatcat
    18181 ttattatttc tcaactactg aaaaaagcag ttgtttgaaa tcttcgatat ttgtgcattc
    18241 aatcaatcga tccaacttct cttcgttgtt gatcaattca attagttctt tcatgatgtt
    18301 cagatgcgag taggagtcta ctgctgccaa acaaaaaata atctttactg gatccatttc
    18361 aggattaccg aaatcgattg gttgctcaat cgtagctacg ctgactccta atttattgac
    18421 cccatcttct ggtcgggcat gggctagtgc catgtgtttg ccaatcacga tatacggtcc
    18481 gtaatcattc actgcacgaa tcatcgcttc gacatagcgc tcttcgacaa tgttttcttt
    18541 aaccaatggt ctagcagcta gttcaatcga ttcctcccaa gtagtggctt tttctttgat
    18601 catgatattg ctgtccgtca aaatatcttt taacataggc tggatctccc ttttgttgat
    18661 ttctaaattg tttgtttcga ataatttttc taacttgcca tagatgattc gatctactcg
    18721 gccaccactt tctttcatga catctattat ttgagtgaat aactccgtcg attcatgact
    18781 gttaggctct aaccgctgta gttcttcatt tcttttcaaa aagtcttcta taaccaagat
    18841 attcgtctca gttaaaatcg gatcgacaac taaaagcggc ttatttcgat aattcaattg
    18901 aaaggtgctg aaaaccagat tgacatccaa tttatctact aattcgattt cttttgaact
    18961 taaaactgca gtaatctcga tctgaggaaa cttttctttc agattctctg ctaacagatt
    19021 tccagtagcc atcccatggt tacagacaac gactgattta taaacataat tcaagtctcg
    19081 cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga aaagctatct cgtcctccag
    19141 tatcttgcca ccaattactt cttcgatcgt aggaacaaaa tgagtgactg cgcggtagat
    19201 tgggaaataa tttgttcgga tgttttcttt caatggattc atgacttgga tatgattctt
    19261 gattcttaca atcagtgcag ccatgtgctt atagagattt tcgaataaag cttcttcctt
    19321 tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg atcaattgga tcgttagtaa
    19381 ttgagcttgt acccactcga ttgaattgga gatatctcta gcattgaacg tctcaatagt
    19441 gaaacgaaga taatttcttt ctacttgcgg gacattgtcc aattgaaatt cctcgatcat
    19501 gtggtcaata aatgcagaaa agctactgtc ttttatttct gtatttttcc aattaatggc
    19561 agagataaat tcatgtttcc tgattcgctg gatccagatc aatgtaaaaa gcaggatttg
    19621 ttttttataa atatcatctt cttgtttcaa caacttattg gaatagatct cctgtatttt
    19681 tttgatttcc accaaaggaa aataatggag aaagattttt tgagccggtg ttaattgtgc
    19741 atcttgattc ataaagaagt cggtcattcc cagattctga ttgataaaat catagatcat
    19801 cgtacggata gatcgttctg aacctttata aaccagtcct tgctttccat aactgacgat
    19861 ctctatccca taactgacca gtgttgctcg taaccgtctc atatcttcat ctaatgtact
    19921 tttagagatt tgaaattctt cttctttttt gtttagatgg atcgggattc ggctgtaagc
    19981 aacggctaaa actagatcaa aaatacgttc gcgccgattc aaaaagccgt ctttcacatc
    20041 ttttatttca gtttctaatc gagcttgctt ttccttttcc agcttcaaca aaaaaacttt
    20101 gttgcgaatt gtacggatct ccggaagctt tttactgctc aaccagatat tcgcttcaag
    20161 aacttcctga cggatcgtcc gctgactgac attgaactca cttgctagat gttttgcttt
    20221 tactggatat tgggctgtca tgattctgtg aagtatttca attgttcttg ttttcataga
    20281 aatgatctcc ttctgatttc aagtatatgg ttccgcttac ataataaaaa gtagatattt
    20341 ctgccacttg aagtggaaag aaagtaagca aagcaaacaa aaaataagcc atatgctttt
    20401 tcatctcctc aacgaaaaag acatacgcct attattacaa aaattttgcc catctataca
    20461 aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacaacta aactaaaata
    20521 gtgcttccta tttttcgttt ggttgttctt attatcgcag atgcaagctt ttaaacacca
    20581 tttagtggag aggttgtgac agagatgttc agccttgagc gataagaagc gattttgaaa
    20641 aatcgcttcc catattttac aaaatcgtgc cttattgccg ataggctatc tctggcacac
    20701 tgtttattca gaaaaaggag ctgcgacaag gcttttgtca caactccgtt cataaatatt
    20761 tcctgtcaat ccagcataat cggctgaata aaatcggctt gccatctttt ttctttctca
    20821 gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt gtgtcttact tcctgagatc
    20881 aatcgcccat cttttcgatc gactgaataa tcacagcatc tgaaatcaat tcggatggca
    20941 aaactatttt cgtggatctg tcgataattt cctacttcct gaatagttat ttttttagta
    21001 tgatttctta caccgtctgc tttgtttttt tgcagaactt tctgatgttc ggcaaacagc
    21061 ttgtctgtat aacagcttct ggctttttca ggagtttctt cttcccaagc ggattggatc
    21121 ttcatgaata tcttttggat ttccagaaac tgtgctttct tttctttagc cgttcctgac
    21181 atgcgggaca atatctccgc tttcagcctt ttcttttgtc ttttattctt gacgatagaa
    21241 aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa gaaatcccgt ttgctcataa
    21301 ctagaaggac ctgtacctgg accataatac ccataagaat ctctgccgta agaacgactg
    21361 ccaaaataac tgcccccata tgaacgacca ccaaatgaac ctctgcccga acttcgaccg
    21421 ctagatgtac ctccaccatg accacccgcg acagcgaatc cttgggtcgg cagagcaaac
    21481 aaacataggc ccactaacaa aatgattgct aaataattga tttttttcat tgtcttcctc
    21541 gttatctttt attacaaaaa actattttta tgatagcctt gctaaaagga agagtcaata
    21601 accagatgca taaactgttt ttttagcaat tttgtcttat ttagtcattg agtaacatcg
    21661 gacaaatgta tgtgtcggca gtccataatt cctctatctt cagcatgaac aagcttgcag
    21721 acaagagatc tgcgcttcct ccaggagaga tttggttttc ctgacaaaag tgcaacagtg
    21781 ctgtaaatgg acgatttggt aaagcacttt gactagcagc atgagcaagt ttttgcattt
    21841 ccactaacgt tttctgatca ctgcgatgca atacgcatgt gtcatctagc gaactcatca
    21901 aaactaacaa catatgcatt tgtttctgta catatgaatc attatctagc tccttagctg
    21961 cttggattgc aagcctgata tgcggatacc ctaatatcgc ttcttcatat gcaccattta
    22021 cgccatattt ttgcttcacc ctatgaccat gagtggtttt tttatgacga tattttgtat
    22081 caggaaaagc agcaagcttg ctgacatcat caagtatttt cgataaagaa aaaggctgtt
    22141 ccatactgat tttttgtgca atgacactga tcaatagccc cattgcccaa atcgctcctt
    22201 tatgggtatt gatgccattt gtagcaatca gcattgcctt ttccgctttt cgcccaatct
    22261 cagcaattct ttctcttaac tcctgatcaa tagcatgatg ccatgctatt tgagccattt
    22321 cttcaaaaaa aggagttagc gtcttagcag acttaagaaa taaagcatag tccatatcgg
    22381 aatgtgcacc cgcatccagt gcatccacta atccaggttt tggacttaat gttacttctg
    22441 cgatcaatgc ttcctctacc aacgcagcga gttgctgagt cactgctttt tttcgttcgt
    22501 catttatcat gacactcact ccttcttcct actattactt gctgaaacct tttttgatag
    22561 ctacccaaat gatatgaagt aaccagctta aagcaaatgc acaacttgcc gtcattagat
    22621 aagctacaag aatcatgact cctgaaggta actgcagcca actatcttta aacaataata
    22681 acagtacttg gaaaacaaat acattggcta aatacgtacg ataagcataa acagacaacc
    22741 atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat tttggaagcg cctatgaaga
    22801 cagcaaagat gatgactaaa ctgtataaac tttggatcgt attcaagtaa ggtgcattcg
    22861 caaagctcaa gtctcctgga tgtgcgagca atgaacggac agaaaagaac gcgataacta
    22921 aggcaacagg taagaaaaga taacgtactc tgtccaaata ttgatagact ttttcgtgat
    22981 aaacaaatgc ggctacccca taaatcatga aactagcaac aaaccgatct aataagtacc
    23041 agctgtcatg ataaggccca gtcaatactt gtgtttgata gaacagatac catgcgacat
    23101 ataaaatcgt tgaaccaatc aatactggcc atacatattt tttattagca aatactttgt
    23161 aaccgagcca aacaaaaaac ggcatgaaca attggatctg cagcatcata acggtatacc
    23221 ataaatgagg cgctccatca cctgtgacat atttcagtaa aaagcttgca gtatccgtat
    23281 acggagtttg ttgttggact gctggaaata gccaaagata agccgtcgtc catagaagat
    23341 acggaacgac tagttcaaaa aacttttctt tcaaaaattc ccaataagac aaatgctgac
    23401 ttgtttttac catattataa acaattgcaa aaataaacat tggtgctgtg tatttaacga
    23461 tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct gtggctgttt gccaaagcaa
    23521 agctcaatat cgtttgcatc ataacagccg ttacagctga tactttctgg aagtcactta
    23581 cgttgatttt gatttttttg ttttcaaaca tctctttctc tctctttttc tttattacca
    23641 aagttctttg gttaatttag gtccttcgac tgttttcaat agaatttttt tctctggctc
    23701 tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc actacttggg tatcgaattg
    23761 ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc aattcaatcg gtgaatttgc
    23821 gtacagcagc aaatcaaaat cactggtttc ttttactgcc ggaatcccag tcgataattc
    23881 aaatcctaag ctgccgccga ttccccattt acatcctgat aatatgtttc tagcagcatg
    23941 aaattgctga taaacaggat aatccatgat tttctcttgt ctatacgatt cttgttcgat
    24001 gatgtcccac ggatgaatga cttgtttgac ggcatgtttt gagatttctg ttgaccatct
    24061 ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt tctgcgtgat ccgtcgacgc
    24121 acgacgaact gttccgaaca tcttttcagt tagaacgacc cattcaggca aggtgaaccc
    24181 ttctatttca tgtttatcaa aaaggacaat atcatgtgct tgaatcattt tttatgcatc
    24241 ccattcttcg tccattttgg cacggacttt atttgtttcg acacgtccga atgttttggc
    24301 tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt gtattgcgga catcttgaat
    24361 ggcttcattg attgcttcga tgactttgct gacattttgt tctgaagcag cttggtcggt
    24421 gatatcttct aggaaatgat agatcgctcc tagttttgta taattattga tatcgtaagc
    24481 gatagctgga actttttcag cagctgcttc gatttcagca accgaacgtt ttgtcacacg
    24541 tgcagcactt tctttagaca tcgcttggac ggtaatttct ttattgttta aagcaatcaa
    24601 gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa atagcatttc ctgcaatcaa
    24661 tccaataact ggatgtccag cttgacggag ttttgcataa gcagcagcac tattagccaa
    24721 agccacatgg ataccgatca attcttcgtt atagccgtat gcttggcttg gtacatcgat
    24781 cacaacgatg attggccgtt tcactttttt gtctttatct tcttcgtaaa cgtgatccaa
    24841 aatttgtccc atgcggaaac cttcaactaa accaacttca ccgtttcgta cacggtacat
    24901 cgggttttta ggatcaggta cgattgatgt cacgatggct tcttctatga atttacttga
    24961 tttaccataa tacacagtac taatagaaga agttggattt ttcatgccag ttagtgccgc
    25021 aaaccaatcg taaccaacac ttgctgcagc tggttcattt ccttctgcta cttctggtaa
    25081 gtcaattgat tttgcttgat ggttagcaat tgcttcattg taatcgtcaa tggccattgg
    25141 ttttgaaaga tctaaagaat caagtaatga taaataaaat gctgagcttt cactgcgatg
    25201 cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct ttgattgctt ctacactatc
    25261 agtcactaat tcatcaatga tttttgattc tacccgttga cgagatccta atgtattcca
    25321 aatcaagtct ttgtcactag aatcaaattc tcgaacgcct gcttcttgtt caataacttc
    25381 tggtccattc aaaccgactc tcgctttttt tgttgcgatt aaataactca ttaatgctga
    25441 agtaatcgac ataccgccaa atgatcctac acgtcctgga atcaaaccga tgactggtac
    25501 atattgtttt agagcaatga ttacatttcc gatttctgaa atagataaca gtccgtagtt
    25561 tgcttcttgc aaacgaacac cacctgtatc tagtacaatg actggaaaga tctcatttcc
    25621 ttttttattt tcttctaaca catgttcaag tgcagcaacg atttttgcac cggaaacttc
    25681 accgataccg ccaccttgga attttccttc catagcgatt actactgctt gtttgccatt
    25741 gatcgtgcca cggctgacaa cgatcccgtc atcactttca gggacgattc cttgtgctgg
    25801 taaacttggc gcgatgattt gatcgaatgg atcaatcaat tcacgtccat tgtcaagtag
    25861 agcgttcact cgttctctgg cgtttaattc tacaaaacta tttttcatct gaaagttcct
    25921 ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt agcaccgaag tcattgatca
    25981 cgatatctgc tgaaattgga taacgatcaa aaaatctcgt taatacattg ttccatactt
    26041 cgccgaagcc atcgcttcct gtacaaattt ttacagtaga ttctgttttt tctgttggaa
    26101 acatcaagat ttccaaatct cctgaaccga ccactcctac atggattttc ttattgatcg
    26161 gctgtttcgt tggatattta taagctaatt gttccatgga tctctcccct ttttgtaaaa
    26221 cagagcatac acgttctgct tattcgtctt tcttcttcca tctgggtaaa tggcgacaaa
    26281 aggtttttct cgccaatttt atgaccaagt tctgaatttc attggtggtt catacaggcc
    26341 atctgaccat tcgatcaaat catcgatggt ttgtgcagct aataatgaac gtttcgcttc
    26401 actacgtttg atgcctagat cttctggtaa tttcacgatg ccggctgcac gcaagctatc
    26461 tagtgattct ttcgtgctag tcattccaag aggtgtaaca ccgccaattg ctgcaattgc
    26521 tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa gcaattcctt cttctgtcac
    26581 gatatgtgta gtatcttcgc tataaatcat cactggtgca ttggctaagc ctgcttgttt
    26641 ttgaacagct agtgcatcta attcttcgac aaagactggt tttttatttg aaccatatgt
    26701 ttcaaccatt tgaacaacta atttttggcc tttccctaat ggatcatcat tgtcgcgaag
    26761 tgattgccat gctggtgtag aatgacgacg accgccagga ttatgtccca tgttaggcgc
    26821 accgccaaaa ccagataaac gtccttttgt tacggtagaa gagtttcctt cgtaatctaa
    26881 ttgcaaggtt gaaccgataa acatatcgat ggcatattgt cctgcagctt gtgataatgt
    26941 tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag acatctgggc gagcagcaat
    27001 atatttttcc ataccaactt cgccaccaaa actatggatg ctttcaaccc aacctgattc
    27061 gatggctgga atcatcgttg gatgtgggtt caatacccag ttcttcgcga ttttcccttt
    27121 taaccctagt gattcaccat aagttggcag taacaattcg atagctgctg tattgaatcc
    27181 aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag attcctttga ttgccatcat
    27241 tgccattaga atttgcaatt ctgtgatgtt ttgaggatct cgtgtgaata aagcttctaa
    27301 ttgataaggt ttatctgctt tcacgacaac gtctacccag tcaccaggga tatctacacg
    27361 tggtaaagaa tcgacaactt cgttgacttg aacgatcacg ataccatctt tgaaagctgc
    27421 agcttcgatg attgttggtg tttcttccgt attgaaacct gtgtacaagt tgccgtcttt
    27481 atctgcttta tctgcagcta ctaaaacaac atttggaatc aaatcgatga ataagcgtcc
    27541 ataaagttcc aaataagtat ggatatcacc taatttcatt gtgccgtctt caatcatttg
    27601 ggcgacacgc aagctttgtg caccagcata agagaaatcg atcttgcttg cgattccttt
    27661 ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac atgatcaaat gcagatcatt
    27721 cactttttct gcatctactt tttcaagact ttctgaaaga aaacttgctt gtttttgatt
    27781 gtcgccttct aatacaactt tgtcgccggg agcaatcagt ttttccatta cttctacgat
    27841 tttttcagtt tctacatact tgccattgag caagttgctg attttttcca atcgctgcgc
    27901 ttttttttcg cgtttagaag cccagttgcg gtttggtttt tccataatta gtcatctcca
    27961 tttttgatat agaaacgtgg cgtcatcaat accatgttgg tctagattga tcgtacggat
    28021 ccctttatct acaccaaatt ttgtatgaat cagctttgtc aaagtattgc cggggggaaa
    28081 ttcaatcgtc agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac
    28141 agggaaagct aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct
    28201 tccatccgta ttttttatat acaaacatct agcatcagat agatggacag actgcatata
    28261 cggcttgaat ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt
    28321 cagcagcttc gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc
    28381 ttcgatactc ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg
    28441 atggtcagga tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac
    28501 tactacgccc attgcataac cgcttggata agcttctttc attaagactg cgcggtgata
    28561 gactagttcc gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc
    28621 accaatcgaa tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa
    28681 ataaaatact gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc
    28741 ttctttatca actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg
    28801 gatatccgct aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact
    28861 catgagcctc gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta
    28921 taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa
    28981 ttatgaaatt gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc
    29041 aagcagccaa agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg
    29101 aaaacgaatt aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt
    29161 atgcagaaga attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca
    29221 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    29281 cctctgtttc tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat
    29341 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    29401 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    29461 ttcctaagaa acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca
    29521 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    29581 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    29641 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    29701 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    29761 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    29821 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    29881 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttc
//
LOCUS       DADOZM010000020        28448 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.020, whole genome shotgun sequence.
ACCESSION   DADOZM010000020 DADOZM010000000
VERSION     DADOZM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.020"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8I47_000772"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8I47_000772"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAZ6322229.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="J8I47_000773"
     CDS             complement(937..1518)
                     /locus_tag="J8I47_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6322230.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8I47_000774"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8I47_000774"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAZ6322231.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="J8I47_000775"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="J8I47_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAZ6322232.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="J8I47_000776"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="J8I47_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAZ6322233.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="J8I47_000777"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="J8I47_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAZ6322234.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="J8I47_000778"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="J8I47_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAZ6322235.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8I47_000779"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8I47_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAZ6322236.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="J8I47_000780"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="J8I47_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAZ6322237.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="J8I47_000781"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="J8I47_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAZ6322238.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="J8I47_000782"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="J8I47_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAZ6322239.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="J8I47_000783"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="J8I47_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAZ6322240.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="J8I47_000784"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="J8I47_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAZ6322241.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="J8I47_000785"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="J8I47_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAZ6322242.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="J8I47_000786"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="J8I47_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAZ6322243.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="J8I47_000787"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="J8I47_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAZ6322244.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="J8I47_000788"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="J8I47_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAZ6322245.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8I47_000789"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8I47_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAZ6322246.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="J8I47_000790"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="J8I47_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAZ6322247.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="J8I47_000791"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="J8I47_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAZ6322248.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="J8I47_000792"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="J8I47_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAZ6322249.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="J8I47_000793"
     CDS             13972..14157
                     /locus_tag="J8I47_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAZ6322250.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="J8I47_000794"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="J8I47_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAZ6322251.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="J8I47_000795"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="J8I47_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAZ6322252.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="J8I47_000796"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="J8I47_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAZ6322253.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="J8I47_000797"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="J8I47_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAZ6322254.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="J8I47_000798"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="J8I47_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAZ6322255.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="J8I47_000799"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="J8I47_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAZ6322256.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="J8I47_000800"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="J8I47_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAZ6322257.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="J8I47_000801"
     CDS             18232..18879
                     /locus_tag="J8I47_000801"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAZ6322258.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="J8I47_000802"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="J8I47_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAZ6322259.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8I47_000803"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8I47_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAZ6322260.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="J8I47_000804"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="J8I47_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAZ6322261.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="J8I47_000805"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="J8I47_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAZ6322262.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="J8I47_000806"
     CDS             20317..21255
                     /locus_tag="J8I47_000806"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAZ6322263.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="J8I47_000807"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="J8I47_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAZ6322264.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="J8I47_000808"
     CDS             21935..22774
                     /locus_tag="J8I47_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ6322265.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="J8I47_000809"
     CDS             22750..23619
                     /locus_tag="J8I47_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ6322266.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="J8I47_000810"
     CDS             23609..24406
                     /locus_tag="J8I47_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAZ6322267.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="J8I47_000811"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="J8I47_000811"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAZ6322268.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="J8I47_000812"
     CDS             complement(25688..28357)
                     /locus_tag="J8I47_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ6322269.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9231 a         5292 c         6045 g         7880 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaa
//
LOCUS       DADOZM010000021        28441 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.021, whole genome shotgun sequence.
ACCESSION   DADOZM010000021 DADOZM010000000
VERSION     DADOZM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.021"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(218..1015)
                     /locus_tag="J8I47_000813"
     CDS             complement(218..1015)
                     /locus_tag="J8I47_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAZ6322270.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="J8I47_000814"
     CDS             complement(1012..2196)
                     /locus_tag="J8I47_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAZ6322271.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="J8I47_000815"
     CDS             complement(2221..3468)
                     /locus_tag="J8I47_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAZ6322272.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="J8I47_000816"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="J8I47_000816"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAZ6322273.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="J8I47_000817"
     CDS             5448..5762
                     /locus_tag="J8I47_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAZ6322274.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="J8I47_000818"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="J8I47_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAZ6322275.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="J8I47_000819"
     CDS             6447..7091
                     /locus_tag="J8I47_000819"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAZ6322276.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="J8I47_000820"
     CDS             7106..7435
                     /locus_tag="J8I47_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322277.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="J8I47_000821"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="J8I47_000821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAZ6322278.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="J8I47_000822"
     CDS             8423..9247
                     /locus_tag="J8I47_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAZ6322279.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="J8I47_000823"
     CDS             9240..9587
                     /locus_tag="J8I47_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAZ6322280.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="J8I47_000824"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="J8I47_000824"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAZ6322281.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="J8I47_000825"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="J8I47_000825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAZ6322282.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8I47_000826"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8I47_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAZ6322283.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8I47_000827"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8I47_000827"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAZ6322284.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="J8I47_000828"
     CDS             15244..16011
                     /locus_tag="J8I47_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322285.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="J8I47_000829"
     CDS             16001..18043
                     /locus_tag="J8I47_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322286.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="J8I47_000830"
     CDS             18324..18473
                     /locus_tag="J8I47_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAZ6322287.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="J8I47_000831"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="J8I47_000831"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAZ6322288.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="J8I47_000832"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="J8I47_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAZ6322289.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="J8I47_000833"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="J8I47_000833"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAZ6322290.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="J8I47_000834"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="J8I47_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAZ6322291.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="J8I47_000835"
     CDS             22890..23972
                     /locus_tag="J8I47_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAZ6322292.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="J8I47_000836"
     CDS             23972..24775
                     /locus_tag="J8I47_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAZ6322293.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="J8I47_000837"
     CDS             24845..25585
                     /locus_tag="J8I47_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ6322294.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="J8I47_000838"
     CDS             25596..26600
                     /locus_tag="J8I47_000838"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAZ6322295.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28408)
                     /locus_tag="J8I47_000839"
     CDS             complement(26645..28408)
                     /locus_tag="J8I47_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322296.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8962 a         5035 c         5858 g         8586 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 g
//
LOCUS       DADOZM010000022        27828 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.022, whole genome shotgun sequence.
ACCESSION   DADOZM010000022 DADOZM010000000
VERSION     DADOZM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.022"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(286..1230)
                     /locus_tag="J8I47_000840"
     CDS             complement(286..1230)
                     /locus_tag="J8I47_000840"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAZ6322297.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1526..2470)
                     /locus_tag="J8I47_000841"
     CDS             complement(1526..2470)
                     /locus_tag="J8I47_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6322298.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2691..3905
                     /locus_tag="J8I47_000842"
     CDS             2691..3905
                     /locus_tag="J8I47_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322299.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3927..5942
                     /locus_tag="J8I47_000843"
     CDS             3927..5942
                     /locus_tag="J8I47_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAZ6322300.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6025..7398)
                     /locus_tag="J8I47_000844"
     CDS             complement(6025..7398)
                     /locus_tag="J8I47_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAZ6322301.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7424..7708)
                     /locus_tag="J8I47_000845"
     CDS             complement(7424..7708)
                     /locus_tag="J8I47_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6322302.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7710..9725)
                     /locus_tag="J8I47_000846"
     CDS             complement(7710..9725)
                     /locus_tag="J8I47_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ6322303.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9904..10209)
                     /locus_tag="J8I47_000847"
     CDS             complement(9904..10209)
                     /locus_tag="J8I47_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAZ6322304.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10298..11329)
                     /locus_tag="J8I47_000848"
     CDS             complement(10298..11329)
                     /locus_tag="J8I47_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAZ6322305.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11536..12411
                     /locus_tag="J8I47_000849"
     CDS             11536..12411
                     /locus_tag="J8I47_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6322306.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12563..13405)
                     /locus_tag="J8I47_000850"
     CDS             complement(12563..13405)
                     /locus_tag="J8I47_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ6322307.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13497..15374)
                     /locus_tag="J8I47_000851"
     CDS             complement(13497..15374)
                     /locus_tag="J8I47_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ6322308.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15371..16792)
                     /locus_tag="J8I47_000852"
     CDS             complement(15371..16792)
                     /locus_tag="J8I47_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ6322309.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16795..20184)
                     /locus_tag="J8I47_000853"
     CDS             complement(16795..20184)
                     /locus_tag="J8I47_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAZ6322310.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20441..21832
                     /locus_tag="J8I47_000854"
     CDS             20441..21832
                     /locus_tag="J8I47_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ6322311.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21905..22552)
                     /locus_tag="J8I47_000855"
     CDS             complement(21905..22552)
                     /locus_tag="J8I47_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6322312.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22560..23246)
                     /locus_tag="J8I47_000856"
     CDS             complement(22560..23246)
                     /locus_tag="J8I47_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322313.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23252..24325)
                     /locus_tag="J8I47_000857"
     CDS             complement(23252..24325)
                     /locus_tag="J8I47_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAZ6322314.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24547..25221
                     /locus_tag="J8I47_000858"
     CDS             24547..25221
                     /locus_tag="J8I47_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322315.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25226..26566
                     /locus_tag="J8I47_000859"
     CDS             25226..26566
                     /locus_tag="J8I47_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6322316.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26715..27701)
                     /locus_tag="J8I47_000860"
     CDS             complement(26715..27701)
                     /locus_tag="J8I47_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAZ6322317.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8115 a         5252 c         5048 g         9413 t
ORIGIN      
        1 tttctttatt cagttaatca aatatgaacg aattagatat gaatcattgt atttgccgtt
       61 gcggaaggat tttttaatta accacagata aacattgata acgattcgct tattgtaatc
      121 aacagattaa atttcatatg ttcatatgta cggatacatt ttcggaccga ttaattttaa
      181 ccattctgtt tatttgctta cctattagat atcaataaaa aactgcttaa cagctatttc
      241 agctgttaag cagttttagg atttttagtt catattcatt gtttcttata atacagtgtt
      301 caatacttct cttaaagcac ttgttgattt gctgaacatt gctttttctt cttcgttgat
      361 tgataattca acaacttcac gaacaccatt ttcgtcaacg attgatggca cacctgtaaa
      421 gatatctttt tcgtcgtatt caccagttaa ataagcagat acaggcaata cagcttgttc
      481 gttattcaag atagctttta cgatacgtgc agtactcata ccaataccat agtaagttgc
      541 ttttttacga tcaatgatct cataagcagc attacggact ttatcagcaa tcacatctaa
      601 ttcatcttgg gcaatacgat ggtctttttc tacaatttca aacactggtt taccaccgac
      661 agttgtatgt gaccatgcag caacttctga atcaccatgt tcacctaaga tatagccgtg
      721 gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct ttacggaaac gagttgtatc
      781 caatgttgtt ccagtaccga taacacgtga tgttggcaga ccagattcgt tccaagcgat
      841 atatgtcaaa atatctactg ggtttgaagc aacaacgata ataccgtcga atcctgatcc
      901 catgatttct tttacgattt ggcgcatgat tgacgcattt gttttaacta aatctaaacg
      961 agattgacca ggtttttggt tgataccagc tgtgataaca acgatatcag catctttaca
     1021 ttcttcatag ccacctgacc atacagccac attttcatcg ccccaagcca taccatctaa
     1081 aaggtccaat gcttcccctt ctgctttttc ttgattcaca tcgatcaata ctaattcatt
     1141 agagattcct tggttgatca ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac
     1201 gattactact ttacgacttg tttttttcat gaataaacag ctccttttca taactatttg
     1261 ttactttcat gaaaagtata gtcttgttta ttcattttat caacatgtta attataatag
     1321 ttgttattat aatttttgat ggaaacgaat tcctgaatac gcataacaga taaatgacaa
     1381 atgtttacac ctatatcccc tgataataca accaaaagtt tagaactgaa aataaatcca
     1441 aaaaaattcg ctgcaacgat ataataagac agcaatcgca cctaaaacaa ttttccttca
     1501 tatgctttag tgaaagattt tcttcttaat gtttatttct ccattcattg ggagtacatg
     1561 ttgaatactt cttgaataat ttgaataaag aagtcgtttg tttatatccg atacgattag
     1621 cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc caacgcaaag tcgaattttt
     1681 tctttagaac cagttcttga aaggaagcat caaaatacat tttcaattta ttactaagat
     1741 agaccggatt gtagccaaaa tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct
     1801 tatcgatata atctaatact ctaagcatct cattttctgc gcatccatct gctgatgaaa
     1861 agctttgttg ttctaataaa tgagtcaatt cgatcagtaa tagtgatgta tagagattta
     1921 acgaacggat cttatgactg tatgtatcaa aataaatcaa cagcatattt tcaatcaaat
     1981 gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt
     2041 cttttgatgc atttatcaaa aaagaagaaa gtagattatt tgtgcttgcc aaatgattca
     2101 atatctctgt agataaggtt tcttctttta ataagatgtt gattaaaata tcctcttgcc
     2161 ctgtgtattc gattctttga gtagcttctt tatcaatgat caccacgtca ctttcttcca
     2221 tctctatcct ttctccatta ataatttgta aacatgtacc gctatacata taattgattt
     2281 caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg acggtgttta ctgatatgaa
     2341 tatttccctg ttgaaataga tcccctcggt ggatttttgg cggtgtctga agatggtaag
     2401 ctaagtttaa atcattgata ttcattcctt gttcacgctg cagagtttcg attgattctt
     2461 cgttacgcaa aaaaaatgtc taattcagct agttccaatt gactccctcc tttttgtaag
     2521 cggtttcttt cgcttattag ttcacgctgt actgctatta tatgcaaagc tatttttggt
     2581 ttcataaaga ctttctatgg ttgggacatg aacttcctgt gattccgcta ttgagagaat
     2641 aaccacttca tactatattt ttgataagcg cttacaaaaa tgaaaggaac atgcttatga
     2701 ataaaaagat gatgaaattt tctctgctgt cagtctctct tattttgact tctgctggtg
     2761 cagtcacgtc tgcaattccc agtatgtcag ctacttatgc cacgcgttct ttatctgctg
     2821 ttgaattatt aacgactgct ccctctttta tggtcactat ttttgttttg attagtagtt
     2881 ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat cggattagtg attgcatcga
     2941 tttgtggtat cctccccctc ttctcacaaa gttacagtat tgtcttagct tcaagagctg
     3001 gactaggtat tggctttgga ttgatcaatt ctttggcagt cagcatgatt gctgcctttt
     3061 atcacggaga tgaacgagcc tctatgattg gttttcaaag tgcatttcaa ggatttggtg
     3121 cagctttaat gacatttgtt gcaggacagc tagtacagtt cggttggcaa tatacctttt
     3181 tagtctacgc catcagctta cctattcttg tcttgtttgt tttgtttgtt cctacacctg
     3241 aaacaactga acttcaatcg catggcacag ccccaacaag acaagtaatc aaaacgaatc
     3301 ctaaaatgat tagttatggt atttttctct tgctggtcat cgtggtatat aatgctgtat
     3361 cgattaaatt agccacagtc ctgacgactt attctctagg aaacgaaact aatgcagcga
     3421 atatcttaac aatcatgcaa ttggcaagta tggctgccgg agtttgtttt ggctggcttc
     3481 agacaaaagc acatcgctat atgcttacac tttctttact actgatggga atcggctttg
     3541 tattgattgc cactgtgata aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa
     3601 tttctttttc gttattcatt ccttacttat tcaaccaagt aagtatccaa gcgcccaagt
     3661 acgcggagag tttcaatact tcaatcttat tggttggcgc taatttaggt actttcattg
     3721 caccatatgg tttgctcttt ctctcttttg cttctcttgg gagtaaaaca cttgctgttt
     3781 tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat tagccttcta gtagtacaca
     3841 ggacgaatag aaagacagtg ataagagtag atgaagaaat aaaagaacaa ttgacaacaa
     3901 aataaatttg atggaggtcg tgtaaaatga caaatgaaaa acataaagat agatggcttt
     3961 ggtatcctgg agactttgag atccgccatg gactgctaca aaatttccaa cgggaagaac
     4021 gaggttttga ctggccagcg tactggtaca tggatgattg ccatcggaac gtcaagttta
     4081 agagatatta ctttcttgat caaccttcca tgtttaaagt gacgatccaa ggagttgggt
     4141 atgtagaaat taatggacaa aaacatccat gtggaaaatg gctgacttgt ccagctggta
     4201 aagcaaaaat tcgtatcttt gtaggacata catcagggct accagcgatg tttatcgagg
     4261 gagacgaagt aaaatcagat attggctgga cagcaagtaa ttttattgaa gaatatccag
     4321 ctggctggag tccgctttat gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa
     4381 aagaatgcgt ccatccagtc actgaaacgg agattggcgg aggaatctta ttcgattttg
     4441 ggcgagcagt gaatggctgt gtagaactaa tatctctgga tgaccatatc gaatctatca
     4501 ctctatgtta tggagagtcc gatgtagaag ctatggatat cgattattgc tactataagc
     4561 aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac
     4621 cagaggtttc tactggaatg gttcaacttc aagtttttca tgaattttta tctctttcga
     4681 aacaagctgc tttttcaagc gattcttctt tactgaatca gatctggtca gtatcagaag
     4741 aagccttcaa gttatgtagt gatttgtttt ttatcgacgg tatcaaacga gatcgctgga
     4801 tttggtctgg agatgcgtat caatcattca tgatcaatca atatcttttt tttgacgagg
     4861 aaatcaataa acgaacgata ttagcattaa gaggacaaaa tgaaatcaag cagcatatga
     4921 atactatcgt tgactattcg atcttgtggt tgattggtat cgaaaaccac tatatgatga
     4981 cgaaagacta tgaattttta cgcatcgtct accctaagat gcaatcatta atgagatact
     5041 tgatggagca aacgaatgaa ttagggttta tttatgggag agcaaaagat tggatattta
     5101 ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc agaacaagtc ttattactga
     5161 aagcctatca ttccatgatc acttgcggag atatcttagc tgaaacatat ggagagaacg
     5221 aaaattatca tactcatctc tatcaagaaa aatatcaact attgaaacaa aacctatttg
     5281 actacttttg ggatgaagaa cttggcgctt ttatcgattg ctatgaatct gggagaagaa
     5341 atgtgacgcg tcatgcgaat attttcgctg ttttattcga ttttgctaac aaagaccaac
     5401 agcaatcgat cttaaagaat gtcttattga atgacactat cacacagatt actactccat
     5461 atttcaaatt ctatgaacaa gatgctttat gcaaactcgg acagacgaat atcgtctacc
     5521 aaacgattct tgattattgg ggagggatgt tatctcatga tgcggtaact ttctgggaag
     5581 aatacgaccc tactcaaaca ggaaatgaaa agtacagtat gtatggagat ccttatggaa
     5641 aaagcttatg ccatgcatgg ggagcaagcc caatctatct tttaggacgc tattttatcg
     5701 gtttgtatcc aacaaagcca ggttatgaag aatttatgat acaacctaat ctagattcat
     5761 ttgaaacact gaattgtacg ctacctatca aagatggaaa gatttcgctt gaaaaaacaa
     5821 aagataagat cgtgattact tctgatagaa caggcggaac agtgaaaatg aatggtaaaa
     5881 gttatcctct tctagcaaaa cagccacttg tcttaacaag ctattcaaaa ataaatgcat
     5941 aaaacaaaaa actgcttgcg acatatcaga agactttaaa tcttcttctg atattgtcca
     6001 agcaattttt attttgaagt ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt
     6061 tccggcaaaa agaaagctag ataatacgac aagtaaaaag aaagcgatga ttccaactgt
     6121 caaggctatt tgaccgtatt ctcctagttt cccaagtaca agtcctggta ctagataatc
     6181 agtatcggca aatgtagaag aagcgatttt aagatctcct aatactggaa ggataaataa
     6241 tggcagccaa ctaatcaata agctattgaa agcagaacct aataccgccc ctcttctacc
     6301 gccagtcgag ttgccgaaga caccagaagc agctccgcag aagaaatgtc ctactacccc
     6361 aggaatgata actgtagttt gcaagcctat catagcaaac atggatagtg ttcctacaaa
     6421 gaagctaaca aaaaagccaa ttaatacagc attaggtgca tatggaaaaa cgataggaac
     6481 atctaatgct ggtttagaat cgggtacgag ttttttagca atcccttgga atgctggaac
     6541 gatttctgcc aacaccattc gaaccccttg aaggacgata acaaacccag ctgtgaatgt
     6601 tccagcttgg atcagtgcaa agattacagc gttgtctcca ttgcttagtt ctctttcaac
     6661 aaatccagga ccagctaaca aagcaagtgc gacatatacg atcgacatca agagcatgat
     6721 cgatacagta gaatctcgca aaaagctgaa acttttagga atctttatgt cctctgtaga
     6781 acggtctttg tttccaaaaa actttccgat ccatccacta gaagcataac cgatatttcc
     6841 ggtatgccct aacgcaactt gattgtttcc agtgatgttc ttcataaacg gttgacaaag
     6901 agcaggcgta atagttaata gtgttccttc gaagaccccg cctaataaga tcatccacaa
     6961 atttgcagta aaccccgcgc ttactaaaat gactgctgtt agacaagaaa cataaagcat
     7021 tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat ctagcaagta aaatattcac
     7081 gatcatccct actagcatga tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc
     7141 cactactgct tcattgctag gaactactcc ttgagtatgc agagctgttt ggaacatcga
     7201 agcaaaaggg gttagtgaat tactgattaa accagcaccg ccgatcagta ctaggaatcc
     7261 gacaaatgtt ttgatagttc cttggatcac ttctgaaaat ttcttcttct gaagaacgag
     7321 accaagcagt gcaatcaaag aaacgaggat ggagggctga ctgacgattg acacaaacaa
     7381 attcaacaat ttatccattg atagttcctc ccttgaatca tttttattct tttgacaaga
     7441 tcttcgttac tttttctttt acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct
     7501 tttcatcagg tatagaagtt agcgtatctt tcaaagaatg ctcaatgaag aaataatcag
     7561 ccatatcgct agatacactt ccaacatccg tatgacttac ttcaaactcg tccacatctg
     7621 ctccaagatc tgcaagtgct tgtttgatgt tcatctctac cataaaactt gttcctaatc
     7681 cactttgaca tactgcaaaa attttcatat cattcgtctc cttttcttat gatcgctaat
     7741 atttcttctt ttgttgatgc gtgtaataaa tcttgaagat tttgtttatt agaaagtagc
     7801 tttgttaaat ttgccaaagc ccgaagatgt gcctcattat cgatagcagc taaacagatg
     7861 aacaaactaa tgggatgggc tggattatca agcagatcca caggttgttg aagtttcagc
     7921 aaagacatcc ctacttcgtt tacgccatct tctggacgag catgaggtaa tgcgatgtga
     7981 tcgccaatat ggataaacgc accatactgc tccacgcgct cgatcatagc tttgatataa
     8041 ctttcttcta ttttattttc tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc
     8101 cagttttttg gttgatccga taattggatc atttcttctg ttaataattc agatagcata
     8161 ggacgttgat cctccttctt ttctaaaagt ttgttcattt tacgatttaa tacttgatag
     8221 agcttttctt cgtctacgcc ttcttttaat tcgatataag gcagtagcgc cgacaacaat
     8281 tcttgggcag aaggcattga aaatcctggt atcagccaat ctctttgtac ttgattgatc
     8341 agctgatttt tttccaagtc agtcatgatt ggatgcagaa catagagaga ctttttctta
     8401 tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg
     8461 tgtaagcggt cagctgaatt agtctctgta aaaaagatca tcgggaataa ttgctgaagt
     8521 tcacttttta agatcaatga agagctaatg ccacttggac agacgatcac ggctcttatt
     8581 tttctatttc tttcttctgc gtccgctttt ctgatctctc cgccaaatag tatagtgaaa
     8641 aaaccaatct cttcttcagg gatgctcttc cctattcgtc tttctaacgg aagtaatgct
     8701 tttcttgtca tttcgtagat ggaagcatat tgatgcttaa tttgatcgat catcacatta
     8761 ggtaaataaa aagaatagga aatacgaaaa taggcaggga ccaagtgata aaagacattc
     8821 atcaataatt cacgaaacga agcaaattct acggccatca ggaattccat gcgatggatg
     8881 acttctgaag aacattccag caaaaaatca agtgcaggct cctggacatc tccttcaacg
     8941 atcgatgcta agcaaagaga gaaaaatata atctccgaat tttgatgttc ggaatagcca
     9001 aggccagtca atacttgtcg actaatgtca gtgactgcaa gagaagccaa cagttcagca
     9061 tcttttacca gttccatgga tgtttttcca attcgaatgg ttgtcaaact caaataataa
     9121 gctaattcat ccattcgatt tggtgcgaat cgaagatctg atagtgaaat cgttttatct
     9181 agcaaatctc taactttggc gtaataatca atggaaaggg ttgcggttaa atacgtgagc
     9241 ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt
     9301 aatattgctt ctgaccctag cagcctaaaa ccagttttcc tagtatacga cagctgacaa
     9361 tcttccactt ctaattcatt tcttatcttt ttgatatctg acaggactgt ccctttagaa
     9421 acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc
     9481 aaataaagaa gatctcttcg ctcttgttcg gtatacacag cttcaaatcg agtaatatcc
     9541 aaaaattttt ctgcccagtc gattgctcgg aatttcttta tacggatacg ggtatcatcc
     9601 acatattcgg caaaaggagt taattgtttg atcgcctcgc ttgtatttac ttcgatgatc
     9661 tctgctgctt tttctagact gatttctctt ggtgttttca ggagataact aattttgtag
     9721 ttcacagcgc tgttcacctc ctacttatat tctagtatgg tagcgctttt aaatcgaggg
     9781 gtgtttggta caacttacag ttggattttt tttacgactg taccgaacga tttctatcga
     9841 atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc
     9901 cgatcaatga tgatcatgtt tattttttga ggtcagttct tcaaattttt ctttgacatc
     9961 ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc tcattaggct ttgtttcctc
    10021 ttctacagaa tttgaaccag ataaagctgt ttctttattt tctgtttcac cagagtttgt
    10081 gccagctaat gattcttctt tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac
    10141 ttcttcttct gttttagggg ccattgaacc tcttaatcgt ttgtgttttt caccgtcata
    10201 atttgtcatg ttggttcctc ctagtagttc tatacttttt catcgtacaa taagacagtt
    10261 tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca tatttttcct tctgattctt
    10321 gaatgctttt gattccttct tccaaaattt tcatttgtaa gattgtttct tcatctttga
    10381 tgactttttc tgcgccatta actaatgtct cgtaaatccc ttcgtagaca cggctgtaat
    10441 caccaacctc ggaaattact ttttcttcat gataactccc ttgttcatct atataggtca
    10501 atacaccata atgttcgggc aaatcgatac caaagtcttc atgatcaggc atgtaaaaca
    10561 ttttcagatg ttcttcttgt cgatcttttg tttgtttcac gaacatccct ttttttccgt
    10621 aaacgacgaa acttggacgt tctttgatgc ggaaataact ggattttatc gaaattttca
    10681 tatttccata gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc cctaaaagtt
    10741 gacgtacatc atagctgata tcatctggtt cgccgaaata agaaagaact tgatctactg
    10801 tatgacaagc atgtccatat aaatagctgt tcgcagtaga aaattcatgc gtattttctg
    10861 gtacttccgg tctgaaataa tcataatgca tttctacttc taaaatatcc cctaattttc
    10921 cgctttcgat tactttttgg actgtcaaga agtcagaatc gaaccgtctg ttttggtaac
    10981 attggataaa tagacctttt tcttttgcta atgcaaataa ctcttctgct tcctttgacg
    11041 tttctgcaaa aggtttctct actagtacat tcttaccatg ttcgagtgcc atttttccat
    11101 attcataatg aaattgacta ggtgtagtga tgacaactaa ttggatctca ggatcatccc
    11161 aaagatcaga cagctgatca gtatattcga tgtcttcgaa tgttttccac actggttcag
    11221 atggggaaaa aatttttttg atattgattt tgttcgtaag ttttcttgaa aaaggcaaat
    11281 gatagcgatt tgtactcttt ccattgccaa gataagcgat tgtaagcatg agtggtgcct
    11341 ccttttattg atgttttcag tatatcctag aaaagcgcag aaagtattta taatagctat
    11401 tctggtaaaa aagttcagaa aaacacttgt tatcattcgg tttggtgaaa ttaatgttca
    11461 aagtaaggat aaaaatcaaa aagatagtag cggaaccaag tgtttatcag taagatagaa
    11521 tttggaggtc agatcatgct tattgaagaa aaactaacaa aacaagagtt gtttaccacg
    11581 acagagaaac ggattgtcga ttacatccgt aggaatatcg aagctgcagt ctacatgacg
    11641 attgaagaat tagcaaaagc tacgtatact tcccattcag ctatcattcg tttatgcaaa
    11701 aaaatgggat tcagcggatt taaagaattc cgtttcgagc tggggcggga agttcatcaa
    11761 ttgattgctc agttcaatca aacgattgat cctaattttc ctttttcaag tgaagatagc
    11821 actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa aaaagcacaa
    11881 ttgcagattg aacatgaaga tttagatcaa atcggtaaag tattgaacaa ggcacagcga
    11941 atctttcttt ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca gaataaaatg
    12001 gtcaagttga ataagttttt gatcatggct gaagaataca gtgattcttc ttggaatgca
    12061 gccaatttga ctagcgagga ttgcgccatt tttattagct acagcggtcg catccatcat
    12121 tatgaacgaa taatgaccta tttgaagcat gtagaggccc ctaccttgct gattaccgga
    12181 aatcagcatt cagaaatggc caaacaggct gatatgtgtc ttgttatatc gcaggaggaa
    12241 tatgattttg ctaaagtagc gactttctcc tctcaaattg ccttcgatta cgtattaaat
    12301 acgttattct cagtgatata ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac
    12361 aaacaagaat tgatgcaaaa aggccttctt aaagaatatc ggaaaactta agaaaagatc
    12421 ctcatttagt aataagaaag atagtgtttg ataggctctc tttgatatca tcataaaaac
    12481 aggctgaagt tttctttttc aaacctcagc ctgtttgttt acggaacgat tcgaatagat
    12541 atattataga aatgaggaat agctaacttc tgtttcttat tttgaacgat tgacgaaaaa
    12601 gatatataag aaggaggaaa atcagtatac atgcagctaa caatccatac aggaaaaatc
    12661 gtttgttctc ggatactttc tgtacttctt tttgaacttc ttttgcatga taaggaaccc
    12721 ggtgccctct gacaagtaag cgatgactgt tgaccatata aggtgtacaa gttaggagtg
    12781 tcacgaaatc ctgccctttt tcgattctca gatcttttgt atcagtcggt tctaccgtct
    12841 tgatctgatc gacttgataa gcgagtgtcc gtccattgat ttcgatatag aaagaatcac
    12901 ctaaagtcag tttcggtaag tctgtgaaca actttgcttg aggcagtcct cgatggctgg
    12961 aaataacggc atgtgtattt tctccccccg taggataaga cgttccttcc aataatgagg
    13021 cacctttttc tagcagcaca gtattcgttt catcaaaaat tggcaaatgg acattgatct
    13081 cagggatcgt caaaataccg atcgtatgtt tctcaaaata ggttctatcc tttttcgcag
    13141 gttctttttt cttagtaaag ggatctgcac ctggatttcc accttctttt gctaattgct
    13201 ggtttttctt tgtcatgttt tcttggattt tagccatgac ttcttcattt tctttgaccg
    13261 cttgttgctg ataatgagag atgatctgct gatccagata attattcagc gcgtcgctaa
    13321 caaatggata agctagtatc cctattcctg agagaagcaa gaaaagcatc agcagatcta
    13381 gaagtttcct tttctttggt ttcatttgtg ttctccttgt gatgactgtt tgataataaa
    13441 agaaaaagga gcatttgcgt aatccgctgc tcctttttcc aattcttgca aaaagactaa
    13501 atctgactgt gcttgcgtct agcaaagtac agtccagcaa tcagtagaag gactactcct
    13561 gcaccgatat acacatagat tcccttaccg cctgttgaag gaagtgtacc tttgtgttta
    13621 tttggtattt tttcaggaga aatcaactgt cctgctgtta cataagattg ttcatcgata
    13681 gtaaatgcta cacggtttgt taatggaaca tatttttctg gcgctttcgt ttcttccaga
    13741 tagtacgtgc catattttag acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa
    13801 accgttgctg attctttcgc cgatacccag ctgacggctt ttgtggatgg gtcgatcgat
    13861 aaatatttcg ctgtgtcact atcttgatca cgaacgacga attctgctcc agcaagtgtt
    13921 tggtctgatg taacgtcacc atctacttta acaaatcgtt tgcccccagt aacgacatcg
    13981 actgacggtg gtgtttgatc atttgtatgc ccgttatcga catttgcttg gttagaaaat
    14041 cctttggttg gatctgcttt ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg
    14101 ccaggagtta atgaaggaat ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc
    14161 tcagtgacag aataatttac tgggtcgatt tctttatttc catcatataa ggcataagca
    14221 tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt catgagtatc gataagtttg
    14281 aatgttgtgt acttgttttg cgttccttct ttatcagcga tacctaatgg gatattgaca
    14341 gaaatctcat attgaatttt ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt
    14401 gttttatcaa ctttagacgt atcatttttg atggtctttt ctactggtgt ttggctattt
    14461 gccaggatct caaaaggcgt gattgtttgc ttttcaatca tttccgcatt atcaggagct
    14521 tttacttctt ctaaattata ttttccaact tctaaatgat taacgatcaa ctggcctttt
    14581 tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa
    14641 tgtttggctt tggtttgatc agttgtccaa gtgtacaatc catctgtgac actttggatg
    14701 tattttttga cgcttggtgt tccttcttct ttagaaataa taaattctgc tccatttagt
    14761 gcttcgtttt cggcagtacc gatttttgta actttcaacg ttccatcatt accgactgta
    14821 tttttagggt agagatggat agtatctagt tcttctgtcc cgtatttata agagccatct
    14881 gcttgtttga tcatctcata tacagggaaa gctaaaacca tgtttgcggc agctgacact
    14941 ccgtcttttg gttcttcttt gatcgtgtag actgcatctt tcccattttg ttttttaggt
    15001 aaagataaag tgacatttcc atcagcatct gtcgttcctg aagcaactgg tgtaccagga
    15061 gtcaaagact ggactgcttg ttttgctgca tccacggacg ctcctttatc tcgttgcgca
    15121 taaaattctt gagtgacgtt ataaactgaa aatgaaatat cggctaatcc ttggtattga
    15181 tcgaattcgc tcatttcttt cccgctgttt tggattaaag gatcgggtaa atcagtcatt
    15241 ttctttttat gaaggatgac ttgagctgct gtctcctctg caaacacttt ttttgcgccg
    15301 aagctgtttg tgagtaatgg taaaataagg aaaaggactc ctaacataac gtttattttt
    15361 ttatgatttt tcattttttc actcctgttc tttttttgat aaacaaccag attgcaaagc
    15421 ttgcgaatac cataccaaca acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa
    15481 gaaatggacg gctattttct tttttattga ctactttcaa tatactgtcc gcctctttat
    15541 cctcagaaag aaaagttgaa atggtaaacg gaatttctgt ttcacttaaa atataaccag
    15601 aaggtgcttc gatctctttt aatcgataac ttcctgtttt caacccggaa attgaaaaag
    15661 cgccattttt atcggaaatc aggtgaagcg catctgattc gttctttgtc cacttgtatc
    15721 cgttggctgt ttgctggagg taatttcctg ctttgttttt aataaggaat actgcgccag
    15781 ctaatcgttt atctggttta tttgtctcga ttttgacaaa ttgcttactc cctgtatata
    15841 cagagacttt ctttgtactg acaccaaaac caaaatccag tgttccgttg ttgttgattt
    15901 ccttgttcgc ctgtgctgta ctgcttagct gcatctgata agaaatggtc agtttttttc
    15961 cagcatattt ttccaacgaa gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt
    16021 gtatgacaaa gccttctgtc agcttttctc cattcgatga aatcgttaaa ctattaggta
    16081 aaaacgttaa tgcttcatcc gcactatctg acaattcgaa cttttgatag tcaaggatat
    16141 ttacaggaat tgtcgtatgc aaggaataag tgattttttc accaatcgta aaatcatttc
    16201 taggctcttc taataccttc tcaaaaggtg gatcagggta gtcgttttct tcatttttag
    16261 gatacaaatg gatagttgaa agtttttgat tgttttgtcc atgtacagga agaacaacta
    16321 ccatattctc tgccttttct ttgacgactt ctggtacttt ggattcaatg aaaagatacg
    16381 ctttatctct tttttcttga tctttagcgg ccagagaaaa agaagcgata ccatcttcgt
    16441 tgttaagagt tgtagttgtt gcttctgcgg taaacatacc ggaagacgca ccgttttttg
    16501 cgatctctgc ttgtgcttct tctaccgttt tgcccttttc ccgtagatgg taaaaagaat
    16561 ctgtgacatc ataaacttgg aatgtgacac catttaatcc tcgatacgtt tgtaataaag
    16621 ctttttcttg tccgtcattt ggatgatttt tcggcagttg cccgtttggg aaaagcaatt
    16681 tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt tgctacctga gtaaaagctg
    16741 gtttaaatag taacaagaag agacacatac taagccaacc aagttttttc atatttagct
    16801 gccccctctt tcttttttca aaaaaagata ccaaatagaa aacgcacaac caatcatccc
    16861 tactagatag attcctaaac ggccaattcc tcccgtttca ggtaatggta cttttgcctg
    16921 attcgtgatg tctaaagaaa tctggttgtt tttggctcct ggtgacagaa cagattcatg
    16981 atcttgtcca tccacttgaa ttgacccatc ttcgtgtata actatagtaa ctggctcttt
    17041 tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc gtataagttc caggttttaa
    17101 attttcgaat agaaaggtat ctgtttcttg tccatctttt ggcgattcta agtccatttc
    17161 tggtccctgc agtctgaatt ttgctccttt taatgtctgt ccgttgtctt cttttttgtg
    17221 taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg agcgtccata cattatttgt
    17281 catctcggta ttttttcccg tgtaaatgat ttttccatat ttatcgattt ggaattcata
    17341 atttcccggc gaagtatcat atccggcagg accagctgtt tcaacgactc gatatttacc
    17401 aggtgtgagt gaatcaaaac tggcaagacc tttttcattc gttgtttggc tgtcaattgg
    17461 ctgataagta ccatttgctt cttttttctg tagggaaaat gctgctcctt taagatttat
    17521 ctctttgtcc gtcccttgca tcgcatactt acgtactgct acaggaactt caacaaaagg
    17581 attttcaata gtcaactgga tcgtatcatc ggaagtagtg actgtttttc catcaatggt
    17641 taccgtacca tcagaagcga tcttgatttc ccaagtagtc tttttgctta atccatgccc
    17701 ttctggagct tttgtttctg tcagcgtata ggtcatttct ttttgcaatt tgacattttc
    17761 tggaagagaa taggttccgt cgccatgatc tgttagtaaa gtatcaatag aatctcctgt
    17821 taatttgaaa acagcgccaa taagatcctt ttcacctgta gaggaatttt tcgttatttt
    17881 caagtttaac ggtgtgaatt gtttagtatt tttccatgtc attgcatcta tttgttgaga
    17941 atcgtatcct ggtacaggta attctttgat tgtttggtaa ctgaatgctt gaccttgatt
    18001 attgtattga ggtagtgata atatctcttg ataactttct ccgctatttg cagataattt
    18061 gtcaatgtct gcacgttccc acgtatttgt tgtatcttta gctggtttaa tgattcgaat
    18121 atatccgttt ttccaagctg cagcatttgt cgtatgttcc cgttgaatct caaaagtaac
    18181 ttgatctgga cgatcagcta gattgttgtc aaattcttcc cataactttt tgatgtgaag
    18241 actgactccg ggagctttag cagatggtat accgaattca gctaattcat tggtatcaat
    18301 gtttggctgg aaagttgtcc tgccgttcat ttgataccag aaatttggat ggaagtcctc
    18361 attttctgtt tggattctca cttggtaatg gatttggatt tcttggtctt ttcctaaata
    18421 gatctgattg ctcttgatgg tatttccttc tatggaaata gatggagtga ccgttgtagg
    18481 acttgtcccc acactcttga ctgaaagtgt accaggctga taaatgaatg gttctgcgat
    18541 tggatcattt atttgtccat tgcttacagt tgctgagatc tgtacagctt ttttggctag
    18601 gtattcagag atatctgttg catgatcagc tgattcatag taaagatcgc ctttttcatc
    18661 tgatgaaacc atttgtcgca tacgagactc tacttctgct tttgagagac cagctgccgg
    18721 atcgctttga agttggatgc caagaccatg tatttcgatt cctcgttctt tgagtgccat
    18781 cgcttctccg atggttgcga taaacgtact gtcgattctt ctggatagat tgttttggtc
    18841 aggtgcatca tagattcttg agattagaga agtatttccc ggccgatctt gcgtattaga
    18901 aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt acccgctgta ctttatagga
    18961 aaatgttggt acaccatccg tcagcaaaac gatcactttt ttatgtccat ttggaacgga
    19021 tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga
    19081 aggtgtaatg gattttactt gatttttcac tgaatcaaat gaacccatct gtactgcacc
    19141 gttactgtag ctatatcctt cgcttgagta gccgacatat cccatattga ttttgtctgt
    19201 gataccgcta tctgctaaag tatcgacaaa acgatcgaca ccaatcttta cttcaccgat
    19261 ccgattattg tcgttcatac ttcctgacca gtctacgacc aatacgagat caagaggcgt
    19321 gatatctttt tgtacatttc cacgagcatt caaataaaca tcaaacaatc cgggtgtact
    19381 tgtttccttt gcatactttc gtatcgcata gtctgcttga ttaccggttc ctccatattc
    19441 gatatacgaa tgggtccgat cattaggatc tccattccaa gaatttatac catcccattg
    19501 atttgttccg tttttattgc cttgatgatc aagaacattt gtatttcctg taggttgcca
    19561 actggcttta ggataagtgc cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt
    19621 agatggcaca ctcacagctg gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt
    19681 ttcgttttct tttgatgatt cttcttctat ttcgtctggc tttggtactg acacatctgt
    19741 atctttctta ttttctgtct catcaataat gtcatattct ttattatcga cgatcagctt
    19801 gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa ctcagatcga tagtttgttt
    19861 agtgtttgat gagatttcca acgtaatttt cccgctattt acctgataag catagccatc
    19921 tccttctttt ctttccacat cacttaagta attttcgact agaccaattt ctttttctat
    19981 cttttcagca ctggtgttat tcgcatttag cttgatgttc agtgcttttt ttccagactg
    20041 atccgaaatg acttcagctg aaattgtttc taaaactgtt ggatcatctg tagtgtctgc
    20101 tattgcagat aatggcacaa agtttgataa actcaatatt atcagcatga aaataacttt
    20161 cagtttcttc cctgtggtcg tcatttttcc cctccttttt ctttttttct gttttgacgt
    20221 gaatagtgaa aattattaaa ataaacaggc gttacataat tttatttatt ttaatatgat
    20281 aattattata cgaagggcgc taaagggaat cacaccaatt ttcataccaa ttggtttcat
    20341 ttatgaagtt gattccttcg attaatgagc gtttcattga aagaaaaggg ttgagggaga
    20401 aaaacatatg atcaggttgt tggaatgaaa ctatagaaag atggaaaaaa tcattcgaat
    20461 tctgtccatt tctgacaagt ggatgactaa aaaagaactt gccaaacgaa ttggtagttc
    20521 agaatctaca tttgtaaggt atatcgaaga gatcaaaatc cgctggggaa cggatgtaac
    20581 gattcaatct tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac cagcttattt
    20641 aaatgttctg atggaaatgt cgcaaagttc tacaaacagt cgtttattaa atgagattat
    20701 tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca gccggtcttc
    20761 ttttacacgt aagttgaaac aatgcaatca aattttagaa agctatttat taaaagtgat
    20821 cgttgaccaa ggttttcagt taactagcct taaaagtgag ctccagttgc gtatctttgt
    20881 tacctttttc tttttaagtt actacggaag acgcacgttg ccatttccaa tcgaaaaaaa
    20941 gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg aatttgtttc aggaagaaag
    21001 cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga gagaatccca
    21061 aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa
    21121 aaaagacttc ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa
    21181 agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga
    21241 aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa
    21301 agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat
    21361 tttcccatac gatacgactc atttgacgat ccgtgcaact tactttgcac agcagatgca
    21421 acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct
    21481 actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa
    21541 tcctgaaatt caccattata cttctgataa aaaaatactg attatcagcg acttaagtct
    21601 tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca
    21661 ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga
    21721 tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga
    21781 tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa
    21841 aaaacagcta tgtataaacc accctgataa aaatttcagg acaaatacac atagctgttt
    21901 ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact
    21961 cttcttttgt ccaattattt gcaatgagat ccgtcaaagt gttcattacg agggtcatgg
    22021 aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta
    22081 ataattccag ccatttttcg ttcattggag aatcagccga tagttctcta cgtttggttg
    22141 tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat
    22201 ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt
    22261 cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca
    22321 aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccccatct
    22381 tgtcaacgat ttctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta
    22441 ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca
    22501 tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt
    22561 cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca
    22621 ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg tttctcatga
    22681 gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt
    22741 ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc
    22801 tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa
    22861 gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact
    22921 agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat
    22981 cctatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagtgagtga
    23041 ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa
    23101 gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa
    23161 aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact
    23221 tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta
    23281 ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc
    23341 cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca
    23401 gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg
    23461 actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc
    23521 caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa
    23581 ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag
    23641 gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt
    23701 tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag
    23761 caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg
    23821 gagaaactgt gtaataagtt tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac
    23881 tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga
    23941 tgatttcatt ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt
    24001 ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt
    24061 acaatccgat tggtgctttc tctcctcctt caatgtattt cagatctcct cttgttaatt
    24121 gtgaagctgc aaatacttga tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat
    24181 catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata
    24241 cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc
    24301 gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca
    24361 aaatgtcact tgttgaatag tgtaactcta aagtgacaat ttgtcacttg tgttgttttt
    24421 ttcttatcaa taaattgtct aagacatctt tcgataaaac cttaacatga gtcataaaaa
    24481 atttatttaa gccttattta tgctttccat gataaactaa agtgaccaaa tcaatgaggt
    24541 gggaaaatga ttaaactatt agtagtagaa gacgataaag tattatccaa taatataaaa
    24601 gaaatactcg ctgcaatcgg cgagataaca caagtttatg atggtagtga aggactgttc
    24661 gagttggaaa gtggagttta tgatcttgca atcttggatt tgatgatgcc ggaaatgaat
    24721 ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac agactccagt attgattttg
    24781 actgcaaaag atagtttgga agataagatt gaaggatttg aacgaggtgc agatgattat
    24841 ctaacaaaac ctttctatcg agaagaactg atcatgcgcg tgaaagcatt attgaaacgc
    24901 tcgctaggtc tctttgatga aaatgttctg aagtataagg atatcagttg tgatttaaaa
    24961 agcaatgaag tcttgtataa agaacaagtt ttgcccatac aaggaaaaga gtttgaattg
    25021 ctcgtttatt ttttacaaaa taagggaatc attttgacaa aagagcagat atttgaccgg
    25081 atctggggat ttgattctga tacgacgatt acagttattg aagtttatat gagcaactta
    25141 cgaaaacatc tgcgaagtac tggattggat aaagaaatca aaaccttacg taatgtcggt
    25201 tatatactgc aaagggtgtg atgcaatgaa tggagatatt acacgaaagc aacgtagtcg
    25261 cttttttgcg acaaatgtag cagcatttgc ttttatcttt tttcttttgg gattcattac
    25321 tttacagata ttaaaccaat ctgcatacag agaaacagat ctgtcattag agaaaataaa
    25381 ttttgattct cgaacagtac aattagagat acaaagatat cagcaaggcg atcccttttt
    25441 aaacccgact caaacggaac aaaacagaac aaacatggaa cagccattaa tggaaccatc
    25501 aaatgaccgt tttaacactc aagtgatctt gtggtctaaa aatggtgaga tattgaataa
    25561 agcagctatt ggtgggcgtt tcaaccaaat caaagaatta aaaatggata cgaaaaatct
    25621 gaataccatc caaaatattg aattaaatga agacagtggc tcatctgccc tctctttccg
    25681 ttcgattacg aaaaaagcgg agacgaatca agatggcgtt gcctacatcc aaattttaga
    25741 aaatacaaat caaatcaaga attcattagc gacttttcgc acgattatga ttttgtgtat
    25801 gattgttttt tggattctct ctattggtat cagttattac ttgtctagtc taagcatgcg
    25861 ccctattatt ctttcttgga gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt
    25921 gcgcacaccg ttaacgatta ttcaaaatag tttggaacat ctttttacca agcctaatca
    25981 tactattatt gaagaatccg aaagcatcgc tcaagcattg tcagagacga ggcgcctcac
    26041 tggattgact tcggatttgc tgacaatcgc tcggaatgat tctgatcagc agctgctttc
    26101 aaagcaaatg atcaatacac aagaatatat cgaacaactt gttaaaccat ttcaagagat
    26161 ggccatcata gatggaaaag agtttattct ccataacgat gcaacattag aagtaccaat
    26221 tgatgaaaaa aaaatccatc aagttttggt tattttgctg gataatgcat taaaatatac
    26281 gaatccagaa gataagatca ttttggattc atctgttaac cataaagaat ggctgattca
    26341 cgtaaagaat actggccctt ctatcagtga agaagatcaa gcaaggattt ttgatcgatt
    26401 ttatcgggaa gatcaatctc gggcgaaaga gactggcgga tacgggcttg gcttggcaat
    26461 tgcaaaacag gtaatcgaac agcatcatgg acagatttct gtatccaatt atcttccaca
    26521 aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa aaataatgct tatccctaaa
    26581 aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca
    26641 gaagtagctt cttcggaaat aagccgaaat ttctttaagc tgaccacttc tgtcacaacc
    26701 tctttagctt acgcttacga ttttttattg cggtcttcaa tgtgtacatc accgcttgtg
    26761 tcaatatcca attcttctcg gctaacatct tctgtcactt tttcaacatc ttcatgttct
    26821 tctttatgaa tcccaacttc ttcacgaata actgtatgtt tgcttacatc tacttgctct
    26881 tctttgattg ggatagtaat cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat
    26941 tgatctgtta ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg tacttctacc
    27001 gtttcagtat cgttgaccac atgtttgtgg atgtcgactt ctccagttgt cacatcatgt
    27061 gtatttacat ctaatttttc ttcttttagt tgaattgtat catcatctag atttggtttg
    27121 cgatcgatgt cttcagtctt gcttgtaccc attgtatcag tctcgccagg tacgtccatc
    27181 ggatcagttc ctggtgtcat attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca
    27241 cctacgtcca ttggatctgt tccagtgcca gtttctaatc cagtccccat tgtgcttgct
    27301 gggctaacag tatcttgtgt atccattgta tcttctgttg tttctggctg acgatagcct
    27361 tttactgtga tgacaaattt tccatcagaa atatctcctc tatattgata taaaggatca
    27421 ttttctggtg tagccgtctc agaatcatag gtatcaaaag aaaaagtatc ttttattttt
    27481 tcccataaag aacggtcttc ttcaccatcc atccgatttt catctgtttc aacatctata
    27541 ccagacaatc cgtcaaatcc tagtctagat gctgcttcat cagtagtata aataacgata
    27601 tcatctcgtt catacccttc gttacgcaac cgttcaatta ctgataatgc ttcttccctc
    27661 gtactatagc ttccgatgac cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa
    27721 tagatttatg attggtatat tgttacttat aaatttattc tataggagac gattgttttc
    27781 tacaaatatt tagattggaa tttgattaaa acaatttaat tgaaattt
//
LOCUS       DADOZM010000023        27541 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.023, whole genome shotgun sequence.
ACCESSION   DADOZM010000023 DADOZM010000000
VERSION     DADOZM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.023"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(68..1960)
                     /locus_tag="J8I47_000861"
     CDS             complement(68..1960)
                     /locus_tag="J8I47_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322318.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1957..3684)
                     /locus_tag="J8I47_000862"
     CDS             complement(1957..3684)
                     /locus_tag="J8I47_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322319.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3700..4350)
                     /locus_tag="J8I47_000863"
     CDS             complement(3700..4350)
                     /locus_tag="J8I47_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6322320.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4554..4850
                     /locus_tag="J8I47_000864"
     CDS             4554..4850
                     /locus_tag="J8I47_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ6322321.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5022..5642
                     /locus_tag="J8I47_000865"
     CDS             5022..5642
                     /locus_tag="J8I47_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAZ6322322.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5734..6900)
                     /locus_tag="J8I47_000866"
     CDS             complement(5734..6900)
                     /locus_tag="J8I47_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ6322323.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6912..7265)
                     /locus_tag="J8I47_000867"
     CDS             complement(6912..7265)
                     /locus_tag="J8I47_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAZ6322324.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7393..8415
                     /locus_tag="J8I47_000868"
     CDS             7393..8415
                     /locus_tag="J8I47_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAZ6322325.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8854..9288)
                     /locus_tag="J8I47_000869"
     CDS             complement(8854..9288)
                     /locus_tag="J8I47_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322326.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9398..11257)
                     /locus_tag="J8I47_000870"
     CDS             complement(9398..11257)
                     /locus_tag="J8I47_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ6322327.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11375..12535)
                     /locus_tag="J8I47_000871"
     CDS             complement(11375..12535)
                     /locus_tag="J8I47_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAZ6322328.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12842..14266)
                     /gene="araA"
                     /locus_tag="J8I47_000872"
     CDS             complement(12842..14266)
                     /gene="araA"
                     /locus_tag="J8I47_000872"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAZ6322329.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14282..14980)
                     /locus_tag="J8I47_000873"
     CDS             complement(14282..14980)
                     /locus_tag="J8I47_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAZ6322330.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14937..16598)
                     /locus_tag="J8I47_000874"
     CDS             complement(14937..16598)
                     /locus_tag="J8I47_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAZ6322331.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16816..18216
                     /locus_tag="J8I47_000875"
     CDS             16816..18216
                     /locus_tag="J8I47_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAZ6322332.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18468..19547)
                     /locus_tag="J8I47_000876"
     CDS             complement(18468..19547)
                     /locus_tag="J8I47_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6322333.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19633..20592)
                     /locus_tag="J8I47_000877"
     CDS             complement(19633..20592)
                     /locus_tag="J8I47_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAZ6322334.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20604..22127)
                     /locus_tag="J8I47_000878"
     CDS             complement(20604..22127)
                     /locus_tag="J8I47_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAZ6322335.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22137..22700)
                     /locus_tag="J8I47_000879"
     CDS             complement(22137..22700)
                     /locus_tag="J8I47_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAZ6322336.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22706..23569)
                     /locus_tag="J8I47_000880"
     CDS             complement(22706..23569)
                     /locus_tag="J8I47_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322337.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23583..24479)
                     /locus_tag="J8I47_000881"
     CDS             complement(23583..24479)
                     /locus_tag="J8I47_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322338.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24654..25925)
                     /locus_tag="J8I47_000882"
     CDS             complement(24654..25925)
                     /locus_tag="J8I47_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322339.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25915..27432)
                     /locus_tag="J8I47_000883"
     CDS             complement(25915..27432)
                     /locus_tag="J8I47_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAZ6322340.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8042 a         5816 c         4751 g         8932 t
ORIGIN      
        1 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
       61 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
      121 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
      181 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
      241 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
      301 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
      361 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
      421 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
      481 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
      541 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
      601 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
      661 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
      721 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
      781 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
      841 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
      901 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
      961 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
     1021 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
     1081 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
     1141 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
     1201 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
     1261 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
     1321 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
     1381 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
     1441 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
     1501 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
     1561 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
     1621 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
     1681 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
     1741 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
     1801 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
     1861 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
     1921 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
     1981 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
     2041 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
     2101 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
     2161 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
     2221 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
     2281 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
     2341 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
     2401 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
     2461 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
     2521 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
     2581 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
     2641 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
     2701 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
     2761 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
     2821 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
     2881 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
     2941 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
     3001 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
     3061 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
     3121 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
     3181 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
     3241 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
     3301 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
     3361 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
     3421 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
     3481 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
     3541 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
     3601 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
     3661 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
     3721 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
     3781 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
     3841 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
     3901 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
     3961 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
     4021 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
     4081 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
     4141 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
     4201 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
     4261 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
     4321 aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca
     4381 ttgtcatatg acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata
     4441 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
     4501 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
     4561 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
     4621 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
     4681 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
     4741 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
     4801 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
     4861 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
     4921 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca
     4981 tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag
     5041 tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt
     5101 taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg
     5161 atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg
     5221 cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga
     5281 ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata
     5341 tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct
     5401 atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta
     5461 atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact
     5521 ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc
     5581 gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt
     5641 aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg
     5701 taccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt
     5761 ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt
     5821 agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga
     5881 agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa
     5941 tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat
     6001 gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga
     6061 tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc
     6121 taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag
     6181 ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg
     6241 tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt
     6301 caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc
     6361 gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc
     6421 tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag
     6481 cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt
     6541 actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc
     6601 gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg
     6661 cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat
     6721 aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt
     6781 aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt
     6841 atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat
     6901 aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc
     6961 ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat
     7021 gatacgtgag ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta
     7081 tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt
     7141 gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa
     7201 atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga
     7261 atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta
     7321 gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag
     7381 gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa
     7441 ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct
     7501 gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt
     7561 actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat
     7621 gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta
     7681 ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc
     7741 gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa
     7801 gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat
     7861 cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta
     7921 actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga
     7981 caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa
     8041 gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt
     8101 gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac
     8161 cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac
     8221 aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat
     8281 aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct
     8341 gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt
     8401 tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga
     8461 ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc
     8521 gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag
     8581 aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga caagactttt
     8641 ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg
     8701 gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt
     8761 cattatcata aaggattttg atcactctat cataaaggta atattacatt tatattagat
     8821 aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat
     8881 tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt
     8941 gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc
     9001 tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg
     9061 tggacgcaaa gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa
     9121 taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat
     9181 tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt
     9241 gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct
     9301 actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact
     9361 tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat
     9421 ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt
     9481 aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt
     9541 acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc
     9601 catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga
     9661 ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg
     9721 tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa
     9781 gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga
     9841 actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg
     9901 tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg
     9961 gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa
    10021 gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat
    10081 ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat
    10141 cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg
    10201 cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag
    10261 gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga
    10321 tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag
    10381 ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag
    10441 gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt
    10501 tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg
    10561 aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa
    10621 aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat
    10681 atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa
    10741 ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc
    10801 gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa
    10861 acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt
    10921 ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac
    10981 cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa
    11041 aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa
    11101 aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat
    11161 tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt
    11221 tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc
    11281 aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa
    11341 agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata
    11401 agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt
    11461 caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg
    11521 atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat
    11581 tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg
    11641 atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa
    11701 ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca
    11761 taagtatacg gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca
    11821 gatgttgttt ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta
    11881 cattgataag tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt
    11941 ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt
    12001 ctaaatctgc ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat
    12061 ccatccctaa gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa
    12121 gaccgatggt tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct
    12181 gttctaaaat attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac
    12241 gggaagataa gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg
    12301 gtgtattcat gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc
    12361 caacaccagc tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg
    12421 tactacgaat aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt
    12481 gtaacttttc tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac
    12541 cccctcctta ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga
    12601 cttcctagaa gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta
    12661 tgacaagtta ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt
    12721 gataaatggt gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa
    12781 agcaaatttc gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt
    12841 tttatctaat aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg
    12901 ttgttagatt tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga
    12961 caccttcatg gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg
    13021 cttcagtcgt tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga
    13081 aatctgccat cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg
    13141 gatcttctct atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa
    13201 gcgtcggatc tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct
    13261 cgtaagtata atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc
    13321 gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca
    13381 aacgttgtac agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg
    13441 taaaagcgtt atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt
    13501 gtacttttac atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca
    13561 attccttgta ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt
    13621 gtacaagatc tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca
    13681 ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta
    13741 ttccaaaact ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga
    13801 tctcaggtcg tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga
    13861 tgaacccata ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga
    13921 tcgttttcca aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta
    13981 gaagctttgt cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca
    14041 cacctgctac ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag
    14101 taactgctaa ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat
    14161 cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct
    14221 gtgatcctac aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct
    14281 tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta
    14341 aaatccgttg aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct
    14401 tgcatacttc atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag
    14461 taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt
    14521 ctttgaatgt ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct
    14581 gttgtgtcaa aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc
    14641 ccatcgcagg aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat
    14701 ggacgattcc tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg
    14761 aagagggatt gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt
    14821 ctgctgtcat ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag
    14881 gatcaaaagc gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa
    14941 gatttgcttg gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct
    15001 tgcaattgat gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat
    15061 atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca
    15121 aacacgtctt ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct
    15181 gctaataacg cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt
    15241 gcctccatag cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca
    15301 tgccccacta aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc
    15361 attgcaccaa aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta
    15421 cctggtgtac gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa
    15481 aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat
    15541 aataccgtga ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca
    15601 aaaatactga cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact
    15661 agttctcctg aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt
    15721 tctaatacga tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct
    15781 acactgttcg tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt
    15841 cctggtctca aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca
    15901 gcttcttctc ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc
    15961 gctttttcga ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa
    16021 ggatcgatag gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc
    16081 tgccaatgga cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct
    16141 ttattcaaaa tagattgata caaatgagcg atactccatc tctgcggaat attgtattga
    16201 aatttttccg ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat
    16261 ggtactagta actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac
    16321 ccaatcgacc cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt
    16381 tttcggtaac tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca
    16441 ttttctagtt tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca
    16501 atcaatacag ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct
    16561 atgacatcta acatactttg cgcatttact tccttcataa tgatttaact ccctccttct
    16621 cgtcaaacct gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg
    16681 tacgtacatg tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta
    16741 cataagttat aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag
    16801 aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt
    16861 ggcgcatttg gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct
    16921 tttttacagc atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct
    16981 tcggtcatgt taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt
    17041 ggacgacgaa aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct
    17101 gcatctgccc ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta
    17161 gctgttggcg ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga
    17221 ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc
    17281 tatgttgcag attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta
    17341 gggctcgctg ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca
    17401 ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt
    17461 cggacaccaa aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa
    17521 aagacaaccg gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt
    17581 gccggtattg gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat
    17641 attccgctga tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg
    17701 atcatccaag gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag
    17761 ttcaatcggc gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta
    17821 ccagctgttt taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt
    17881 ttatgtattt atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata
    17941 ggcgaaatat tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat
    18001 tggattggtt ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa
    18061 gaaatcgttt tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag
    18121 atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa
    18181 acttatccta aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag
    18241 cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca
    18301 gcttctcatg tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag
    18361 tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt
    18421 gcgacgagta accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt
    18481 tctttcactg tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt
    18541 cctttttcaa tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga
    18601 tgcgtcaatg ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct
    18661 acgatcgata ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa
    18721 ctagctattt gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt
    18781 tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc
    18841 gtcaaaaaac gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt
    18901 ccttgaagat cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa
    18961 tattctgctg ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac
    19021 tcttcataag cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa
    19081 agtgctaagt ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc
    19141 ccataagcaa tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg
    19201 gctaaaagca atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac
    19261 ggaaaaatat aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat
    19321 ggtgtttttt ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc
    19381 agatagccct catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca
    19441 tatatttctt gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga
    19501 aggatctttt gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct
    19561 ttttatttgt acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa
    19621 ggtttcttgt gctcataagg catacggaac aggttcacca aattctggaa atccttgctc
    19681 attccacgta aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt
    19741 ttggttcttc tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac
    19801 cgtaaacgag ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg
    19861 ctccgataat ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag
    19921 caggcccatg gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc
    19981 attgcgagta aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata
    20041 ctcaggtata gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga
    20101 tagataaaga ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag
    20161 ctttccttga gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat
    20221 ttgtcccttt tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa
    20281 catgcggtga tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat
    20341 ataccatttt ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc
    20401 ttcttcatga gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt
    20461 tgcccttctt aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc
    20521 atctgtatgt ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag
    20581 tgtcttcatc ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat
    20641 ttttctatcc ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt
    20701 tttatttttt ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg
    20761 tagtgctctc cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca
    20821 tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc
    20881 acataaggaa cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc
    20941 aatacttgac ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc
    21001 catgcttgtc ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga
    21061 caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat
    21121 aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta
    21181 tgtctttttt ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc
    21241 tcacttctca aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga
    21301 tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt
    21361 ttagtcaagt cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca
    21421 ttctcataag cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc
    21481 gtagagcttc caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag
    21541 gcttcatttg ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg
    21601 tctagctcat tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt
    21661 attcttaaat cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt
    21721 gcttcatcaa ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga
    21781 ctgatccatt taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct
    21841 aaatcaattt tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca
    21901 gatgtaaagt ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag
    21961 aggacatctt gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct
    22021 gtatagacga cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct
    22081 cctaccgtaa aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac
    22141 ctttcatttc ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata
    22201 aaaataaaag gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa
    22261 agaatatttc tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa
    22321 aatgtgatat ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa
    22381 ataagtaaaa ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga
    22441 ttaacaagaa aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt
    22501 tgctttattc cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc
    22561 gccattacat aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg
    22621 attacccaga gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat
    22681 ttagtctcaa ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa
    22741 caaaatattt ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc
    22801 cagccattac aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg
    22861 gtaaagttct catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac
    22921 tattcatcag cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga
    22981 ttttcaaaaa aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat
    23041 ctggtaaagt aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag
    23101 gaatgatcaa acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag
    23161 gaatcatgaa gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg
    23221 attttccttt gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag
    23281 aagtgatcac tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga
    23341 tcgttgtcca aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg
    23401 gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa
    23461 acgcaataac caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa
    23521 ctgcttttcg actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc
    23581 tcctatttca tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt
    23641 gcaacaatga taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct
    23701 gtaaatcctc gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca
    23761 gcggttgcaa catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata
    23821 caaaaaccta aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt
    23881 gttgcaccat ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc
    23941 aagagaatcg tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc
    24001 atagcaaggg ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag
    24061 taaggaatcg cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc
    24121 aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt
    24181 ttagataatg ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg
    24241 gtgaaatata aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg
    24301 aaattttcaa aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta
    24361 atagcaaatg ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga
    24421 gttaaaaaca aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata
    24481 tagtccacct catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat
    24541 aagatatccg aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg
    24601 atcattcaga tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt
    24661 tgcagtaatt gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct
    24721 gctaataatt tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta
    24781 aaacctggta aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga
    24841 tcatttttta cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg
    24901 atactccatt ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct
    24961 tcttttttat tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct
    25021 gtttttttgt ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt
    25081 gctttcaatc cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc
    25141 attaaattgt ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc
    25201 attttttgga tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta
    25261 taatcatcgt tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt
    25321 ccatccttat tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc
    25381 atttctgtca attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta
    25441 aacaaatcat tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt
    25501 ttaccttcta cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca
    25561 tcatattccc agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa
    25621 tctggataat ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt
    25681 ttttcattca aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt
    25741 gttttattaa attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca
    25801 ttccaaaaag tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg
    25861 caagctccta aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct
    25921 ttcatttttt gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg
    25981 tttcaataaa caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt
    26041 tacttgttca cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa
    26101 atcaatcact ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc
    26161 tccacggtta actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa
    26221 atacggcact tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa
    26281 cacctctcct tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca
    26341 agcttctccg tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca
    26401 agcaatcttt actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa
    26461 tgcatcttca aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc
    26521 ttgctcatct tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat
    26581 ctgcttctta ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat
    26641 gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc
    26701 ataatattga tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa
    26761 ctcccaatca ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat
    26821 tgaatcatct acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc
    26881 cgctgtttta tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt
    26941 aattccaaaa gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt
    27001 aggaaaatta caatattcga ctaaattacg agctgcatct actccacgag tacccagatt
    27061 gacagccata ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc
    27121 aacttcattc gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat
    27181 gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat
    27241 catgggaatg ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt
    27301 agctgatgga tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat
    27361 aaatgaactg tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat
    27421 tgtggcttcc ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc
    27481 gttgtcaata atataaatgt taaacttact tattttttat cgattttaat taaccaaaat
    27541 a
//
LOCUS       DADOZM010000024        26707 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.024, whole genome shotgun sequence.
ACCESSION   DADOZM010000024 DADOZM010000000
VERSION     DADOZM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.024"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            180..2108
                     /locus_tag="J8I47_000884"
     CDS             180..2108
                     /locus_tag="J8I47_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAZ6322341.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2246..2701
                     /locus_tag="J8I47_000885"
     CDS             2246..2701
                     /locus_tag="J8I47_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAZ6322342.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3171..3440
                     /locus_tag="J8I47_000886"
     CDS             3171..3440
                     /locus_tag="J8I47_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322343.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3840..4094
                     /locus_tag="J8I47_000887"
     CDS             3840..4094
                     /locus_tag="J8I47_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAZ6322344.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4183..5229)
                     /gene="holA"
                     /locus_tag="J8I47_000888"
     CDS             complement(4183..5229)
                     /gene="holA"
                     /locus_tag="J8I47_000888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAZ6322345.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5267..7540)
                     /locus_tag="J8I47_000889"
     CDS             complement(5267..7540)
                     /locus_tag="J8I47_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAZ6322346.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7533..8072)
                     /locus_tag="J8I47_000890"
     CDS             complement(7533..8072)
                     /locus_tag="J8I47_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAZ6322347.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8208..8426)
                     /locus_tag="J8I47_000891"
     CDS             complement(8208..8426)
                     /locus_tag="J8I47_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAZ6322348.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8423..8668)
                     /locus_tag="J8I47_000892"
     CDS             complement(8423..8668)
                     /locus_tag="J8I47_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ6322349.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8784..9512)
                     /locus_tag="J8I47_000893"
     CDS             complement(8784..9512)
                     /locus_tag="J8I47_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAZ6322350.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(9671..10714)
                     /locus_tag="J8I47_000894"
     CDS             complement(9671..10714)
                     /locus_tag="J8I47_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAZ6322351.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(10704..11195)
                     /gene="coaD"
                     /locus_tag="J8I47_000895"
     CDS             complement(10704..11195)
                     /gene="coaD"
                     /locus_tag="J8I47_000895"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAZ6322352.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(11192..11752)
                     /gene="rsmD"
                     /locus_tag="J8I47_000896"
     CDS             complement(11192..11752)
                     /gene="rsmD"
                     /locus_tag="J8I47_000896"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAZ6322353.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(11871..12209)
                     /locus_tag="J8I47_000897"
     CDS             complement(11871..12209)
                     /locus_tag="J8I47_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAZ6322354.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(12193..12630)
                     /locus_tag="J8I47_000898"
     CDS             complement(12193..12630)
                     /locus_tag="J8I47_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAZ6322355.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(12620..13744)
                     /locus_tag="J8I47_000899"
     CDS             complement(12620..13744)
                     /locus_tag="J8I47_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322356.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(13765..17193)
                     /locus_tag="J8I47_000900"
     CDS             complement(13765..17193)
                     /locus_tag="J8I47_000900"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAZ6322357.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(17219..18382)
                     /locus_tag="J8I47_000901"
     CDS             complement(17219..18382)
                     /locus_tag="J8I47_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ6322358.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(18385..18687)
                     /locus_tag="J8I47_000902"
     CDS             complement(18385..18687)
                     /locus_tag="J8I47_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAZ6322359.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(18801..20573)
                     /gene="recQ"
                     /locus_tag="J8I47_000903"
     CDS             complement(18801..20573)
                     /gene="recQ"
                     /locus_tag="J8I47_000903"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAZ6322360.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            complement(20834..22663)
                     /gene="typA"
                     /locus_tag="J8I47_000904"
     CDS             complement(20834..22663)
                     /gene="typA"
                     /locus_tag="J8I47_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAZ6322361.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            complement(22831..23598)
                     /locus_tag="J8I47_000905"
     CDS             complement(22831..23598)
                     /locus_tag="J8I47_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAZ6322362.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            complement(23604..23878)
                     /locus_tag="J8I47_000906"
                     /pseudo
     CDS             complement(23604..23878)
                     /locus_tag="J8I47_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
     gene            complement(24040..25446)
                     /gene="lpdA"
                     /locus_tag="J8I47_000907"
     CDS             complement(24040..25446)
                     /gene="lpdA"
                     /locus_tag="J8I47_000907"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAZ6322363.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(25454..26662)
                     /locus_tag="J8I47_000908"
     CDS             complement(25454..26662)
                     /locus_tag="J8I47_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAZ6322364.1"
                     /translation="MNEDDTLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVL
                     VEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKD
                     VDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPA
                     KPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEV
                     AAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLY
                     VPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVI
                     NYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLL
                     ADPELLMMEG"
BASE COUNT         7542 a         5637 c         4624 g         8904 t
ORIGIN      
        1 ctcttatttg agaaatgttc tggtttttta ttttctttaa taataatcat tataaataat
       61 aaaaacaaca tcaagcttaa tcaccaaatg ataaccatta tcgttctcat ttagctgtta
      121 ttattatcaa ttacattttt attatgctat ccttgttttg acaaggaggt ctaataacga
      181 tgactaatca gaaaaaagct tttgcagcaa caatcggctg ccttttattt atgatcttcg
      241 gttttatctt cgaccgattc tctcttacca tcaccccact tttttatatc agtgccatta
      301 tcatcggtgg atataaacaa acatcggaag gcttgaaaga attgtggaat gatcggacac
      361 tgaatgtgga tttattgatg gctttagctg ctattggtgc ctgtttgatc ggtaattact
      421 ttgaaggagc aatgttgaca ttcatctttt gtttaagcgg tgcattagag gaatacacaa
      481 ctaataaaag tcaaaaagaa attactgctt tgatgaatct tcaaccacaa aaagcccaat
      541 tattaaaaga aaatggacaa atgcaagaag tagatgtttc tcaactaaag gtggataact
      601 tggtatttgt cgctaaagga gcagctgttc cgatagatgg ctttttagaa agcacacagg
      661 caacgatcga tgagtctgca ttaagcggtg aatctgtgcc tgtagaaaaa tatttaggcg
      721 atgagatgta tgcaggaacg ctgaatcaag gaaatcccat cacgattcga gtaacaaaaa
      781 cgagttccga aactgtcttt gctaagatta tccaattagt agaagaagcg cagaatacgc
      841 caactcaaac agcttcattt atcgaacgaa tcgaaaataa ttatgtaaaa ttgattttgc
      901 ttgctgttcc tctaatgatc cttctccccc actttttcct aggttggagt tgggatgaaa
      961 gcttttatcg aggaatggta ttattagttg tggcttctcc ttgtgcgctt gtcgcatctg
     1021 ctacaccagc ttctctagct gctctttcaa attctgcaaa aaatgggatt ttaatcaaag
     1081 gcggtatcca tttagaaaag ctttctgaat taaaagcgat tgcctttgat aaaacaggaa
     1141 cgctcactaa aggaaaacca gttgtaactg acagcttttt ctttgatgaa aaggatttgg
     1201 cacaacaact tcttgttgct atggaacaaa aaacgacgca tccacttgct catgcgattt
     1261 tgcaatattt cgattgcaca atacctgaag aaatccaata cttaccaata gaagaaatta
     1321 ctggttttgg attacaaact acctatttag gtgcgcaatg gaaagttggc aagcacgcat
     1381 atgatcctga gacgatgata atcagcaaag agattgcgga aatgatcgaa cgtttggaaa
     1441 atcaaggcaa aaccgtgatt tatctttcta aagatcagca gttaattgct gttttaggtc
     1501 tgctagatat tccaaaagca aatacccaac aagtaatttc ctactttaaa tcccaaaaca
     1561 tccatacaag tatgataacc ggcgaccatt ctggcacagc aaaggccatt gctgaacaag
     1621 tagggattga acattactat gcaagctgta ctccggaaga aaaaacacaa ctagtcaaaa
     1681 aagagaatga actttaccaa gtaaatgcga tggttggtga tggagtcaat gatgcgcctg
     1741 ctttagcagc cgcttcttta ggtgtcgcta tggggcaagg gacagatgcc gctatggata
     1801 tcgcagacat cgtcttaatg aaaagcgact taaataaatt tgtctacagt catcggctat
     1861 ctttaaaaat gcggcggatc atcaaacaaa atattatttt ttcgattctt gttattctct
     1921 tgttgattgc ttctaacttc ctgcaatttt taaatctacc tcttggagtt gtaggacacg
     1981 aaggaagcac aatattggtt attctgaatg gcttgcgttt actaagacct ttacctgatc
     2041 tttctgattc ttcaaaaaaa tgtaagtctt gtccactata caaagcgcgg ctgtcttctg
     2101 tcaaataagc aaagtgtttt tttatgattt tgtttatttt atgaaaaaaa cagcttatga
     2161 ataggtcttg aaggatgaat tcctactttt gtaactttct aaaaacatgt tatgattgtt
     2221 tcgaagaaag tgaggaatat atttcatgga aagttggctt tttttaggaa ttattttact
     2281 tgtagcaatt ttagctaaaa atcaaagtct gatgatagcg gcagcttttg ttttgattat
     2341 gaagctcata ccagcctccc gttccttttt cccttggctt caaggcaaag gaatcaatat
     2401 tggggtgacg atcatcacag cggctattct aatccctatc gcaacaggag aaatcggtct
     2461 acgtgatcta ttagaagcct ttaaatcccc tattggctgg gtagctgttt tttgcggtgg
     2521 gctagtggct gtcttatctt ccaagggtgt aggattgatt gcttctgatc cacaaatcac
     2581 tgttgcactt gttttcggca cgattttagg tgttgtcttc ttaagaggaa tcgccgccgg
     2641 accggtcatt gcttctggta tcacttactg tatcttacaa gtatttgctt taggaaaata
     2701 gaacaaattt tgtctatttt aggagattct taacaccccc aatttactga tccgtgatat
     2761 actgacaacg aggtgataca atgttcgaaa aattaaagta tttcatagga acaatcggct
     2821 ggtttcttct agcggtactt ttgattctgg gcttagctta tctattattt cctgatttga
     2881 tacaaaatgt gtttgatact gctttttgat tgacctatta aaacgatata ggggatgctc
     2941 ctgcgatgca ggggcttttt ttaatcaatg ttttgtaata atttaggccg tgccaatgag
     3001 acttcctata gaattcgatg ctcttttttc tttttggctc ttctgctctt ttttgttata
     3061 ctagattaca gtggacaaaa gaaagaatga tcgcatccac ttcaacgaat atcaaaatgg
     3121 aagaattagt gcctgttttc aggttacttt gaaaagaaag gaagacgctt gtggaaactt
     3181 ttgaaggtct tttaattgat acgtacgttt tacaagaaca gccaatgatg ctgactggga
     3241 tcaacttgca gcttgaggac cgtgtgatca atggcctgct taaagatcgc tatttggcaa
     3301 aatatatctt acgtttgccc ctaaataaat atgtgctgga agtaagtggc aaatggaacc
     3361 gtcggaacca gttggtcata aaaaaaatgt tgataaaaaa tatcgatgaa tacattaaaa
     3421 taatcggtac accagaatag cctttactga tcaaaggata ctggttataa aataactgca
     3481 gtttcttaat agaaaaacaa agaacaacag ggaatcgtat cccttttcaa aaaaaacttt
     3541 gcaaacggtt ctctttgtta tataataact tagtaccaaa cattcccatt caaactttgt
     3601 ttaaatagat aaataaaaat ctagtttggt ttgacgctat tgcctcccac aatagcgtct
     3661 ttttgatttt tttcagcttg tactatccgt ttttactgaa aaaaaatcct aataaaagac
     3721 tgttaaggaa aaaaacttgt catgttcata ttgcaaagca cgctgccttt aggtataata
     3781 atgaatgttg aattatgcgg taacacgcgt aagaacttaa gttatcggag gtgaaactaa
     3841 tgccaaacat tgaatctgca atcaaacgtg ttcgtacaag cgaaaacgct aatgtgaaaa
     3901 attcatctca aacaagcgct atgcgtactg caatcaaaaa atttgaagat gctgtagctt
     3961 caggtgctga caacgtagat gcgttatata aagaagctgt taaagctatt gatatggctg
     4021 aatcaaaagg tctaatccac aaaaacaaag ctaaccgcga caaatctcgt ttaagcaaaa
     4081 aaatcgctaa ataaaagaat cggcttcggc cgattttttt atttgtcaaa aaaaggacaa
     4141 aaacaaacga ccttcccgca tgctttgccc tcgtctttct tcttatcgat ttcttaacaa
     4201 ctgccctgtg cgcaaaacga atagttgaaa acttaattct ttttcgattt gtcccgtttt
     4261 gatttgataa tccatctcaa ctaattgatc aaacaactgt accagcaaat gttcatcgaa
     4321 tttttttact tcctgcatgg caagttttac acgatatgga tggattttca atgtttcagc
     4381 aatatttgcc tgctgataac caatctttac aagaaatttt gtctgtaaaa ataaacgtaa
     4441 ttgagaaagc aaaatcgcat taattttgat tgtttcttcc ccttgcataa ctaaatcttc
     4501 gtataaacgc aaagcttgtt ccgtcttccc ttttaaaata tattgggtca tgtcaaaaat
     4561 attatgttct aacgttttag gaactaattc ttctacttct cttaccgtga tttgctgttg
     4621 attttgtcca taaaggacca atttctgtaa ctctctcatc attttggata aatctaagtc
     4681 ggtcaacctc aagaataaat caattgcact tcgttccatt ttgattgagg aattctctaa
     4741 ggtattcatc agatattgac gtacttcttt ttcattcaac tgttggacat tgatgataat
     4801 tgctgttttt ttcaattgtt tcgttacttt tttccgttca tccaactttt catatggcgc
     4861 aaagaatact aatacagtcg attctaatgg ttctttcaaa taagccgtca ataaatcagt
     4921 attttgttcg attccattgt ttactttttc acctgttaaa aaataaggat tctctacgaa
     4981 tactaaccgt tgatctccga aaaacggcaa cgtctctgct tcagctacta cctcatctaa
     5041 acttgcattt tccatgtcaa aacttaaaaa atttaagtca tcttcgccct ctaattggat
     5101 ttttttgaca atttctgtcc gaacttgatc ttggagaaac ttttctgttc ctaaaatcaa
     5161 gtaacaagat gcaagttttt ctttacgtat tttttgtaac tctgcttgta tactcaaatt
     5221 actcaccact ttcaaaaatt atcctaccat gagcagatga gaattactag tctatcattg
     5281 tttccactag cccatgagga tgaaaaactg gatgccagcg ataggtgatc attccttgtt
     5341 gatccgttcg ccatgttttt atctgatttt cttctagtgt ctccaatact tcgctgtgcg
     5401 ggtgaccata acgattattt acgccgcagg atataatccc atgttttgct tcaatatggg
     5461 aaagaaaatc tgtgttactt gacgtacggc taccgtgatg acctaatttg actacatcgg
     5521 ctttcagatt aggataaatc aaaagcaatt gtttttccat tgcttgattc aaatctccta
     5581 aaaataaaaa gcgcctattg ttatattcca tccatagacc aatcgaatct tcatttcttc
     5641 cctctccctt ttcaaaagga gtcaatacat aaagatcata ataaccgctg atcctgtccc
     5701 catgtttgat ttcaatgatc tcagtaccat ttttttgagc ttcagataaa tgcctttgta
     5761 atggctcatt tcgtccagcg ccccaaccga tataaatttt tttgatagta aaatgctgag
     5821 ataacgtggg taattctccc acatgatccg tatcgtcgtg tgtcaagatg agtttatcga
     5881 ttacacttat cccctgacct tttagatatg gaacgatatt tctttcagca ggaggacgta
     5941 tcataccttg cgcccacttt tctctttcaa agtgtaactg acctccagta tcgatcaaaa
     6001 tcgtttcttt tcgaaaagga ctttgcaaaa agatactgtc tccttgaccg acatcgataa
     6061 aagcgatcgt tgaagaaaag cgtaaaaatg gcaaagtcaa aaaaataaaa gaaaaaagac
     6121 caacataaaa caagtgtttc acaggttgtt ctttcaacct atccatacaa atcagtaatc
     6181 cgaaaatata catcccagat agcacaaggg gcaaacgtcc tataatgaga gacgaccctg
     6241 aagaaaaatc gattattcgc tcaaataaga ttaatccctc ttctatcagc aaaagtaaag
     6301 tttccggcag ccagcgattg aagagtgtga gaaataacac accaggaaga aaaagaaact
     6361 tgaataccgg tgcaatcagt aatgtaaaaa gactacctaa aataggccac tcataaaaat
     6421 gccatacaat caacggcatc gtgatcatag taaacaggac gtgagaggcc gtttttttca
     6481 accaatctcc atcaaaagaa atcaggataa acaaaaaact catgaaaaaa ctaagctgtc
     6541 cacccgcatg aaaaagaaat cgaggatcaa atagtaaaag aagccaaaag acaactgcaa
     6601 ataagtccat ggtggaataa tgcttattca gcaatttgac tagcaatcgc aataaaaaca
     6661 gcaaactagc acgcaaaaca gaaatagaac ttcctgcaat agaaacataa cagatagtta
     6721 gtagactcaa cggaataata ctgacgtgta gtggccatcc gatacgtcga aataaataat
     6781 acagccaccc taaataaaat tggatgtgta gacccgatag actgaacaga tgcagcaaac
     6841 cactctccct aaaaatttct tctggttctc tgaatcctgc atctttatag ccccataata
     6901 aagaattaat atatacactt gttttttgcg gaaattcaga ttggacaaag gagataaaag
     6961 atcctctttt tctctgaagc cagtttcgcc agtgattttg catggtgatc tgttcaatct
     7021 tttgaatttg gaaccgtcca gaaattctat ggttcctgaa gtaagcttct tggtcaaaat
     7081 tataaagatt ccgctttttt gctggagaga tgaactcgcc ttcagcagtc aattctattc
     7141 cctttcctcg gaagttaagc catgttttca attctgcttc tgtgtttact tgataagaaa
     7201 gctggagctt ttgtttattc tctgataacc ttccttccat tgtcaaaaaa ttaccatttg
     7261 cacgaatcgt atctgtaaat acagtaattt gcgcagatgt tgttttttcc tctaaaaatg
     7321 aatactcttg tagcctataa gaaatggata ccgttatgct acacaaaata cacaagtacg
     7381 cgataaaacg tccttctttt agcgaaaacc ataaaaaaat tccggcaaga agcaaacgat
     7441 aaccccaatt gtcagtgtaa tacaacaaac agataatcat cgacgtaatt gccggaaaaa
     7501 tcaaccgatt tcgaacatgc ctttctaaaa cgctagacat gttttcctgt taattcattt
     7561 tgaagttttg aaaaatgctg tgagtctaaa gcaacttggt gtacagatac acctaattgt
     7621 tcgatcaacg cttgggcata aggatcattt cgatagtcat ggagataatt gatccctttc
     7681 acaccagctt gcaacaaagc ctttgtacag gccaaacatg gaaaatgtgt gacatagatt
     7741 tctgaacctt ccgtcgggat accaagtttt gcacactgta ggagtgcatt catctctgca
     7801 tggatcgttc gcaagcagtg tccatccacg acgtaacacc cttcatctat gcagtggaca
     7861 tctccactga ctgctccatt atagccgcca gcaatgatcc ttttttctcg aacgagtgtc
     7921 gctcctactt ctaaacgtgt acacgtacta cgtaaagata acagtactgc ctgagccata
     7981 aagtactggt cccaaggaat gcgttcatta tgtgacttat cagtaacttt ttctttattc
     8041 gtatcgcatg cttttgaaga tttttctaac atgttttata ttcctccgtt ttttcaagtt
     8101 gattaaaaag tactcattgc ttaaacaaaa tattctttaa atataacgaa tcatcctgtc
     8161 tgacaaaaga tcctaccctt cactgccgaa atgaagtccg tcatccatta gcccattata
     8221 aaaattcttc gaatccattt gttcaagaaa ttccgtataa aaatcgatac ttcacaatca
     8281 tggcgttttg ctacttctgt gagaacagag atatattctt tgatcaactt gttattccga
     8341 tatttttgtt ttgtctcatc gactgctggt ggcgtgataa agaaagcatg atcagaacta
     8401 tagtcttttt tcagctgaac tatcatccac tgcagatttt ttggtacgtt tctttagaaa
     8461 gttgtttatg ctctgctaga tcattcgtcc caatgagaat aaatacttta tctgctgtat
     8521 agctaagtac atctttggca aagcgttctc gtagagcttg actgttattg ccgctgacag
     8581 ccgtattcat aatatgaatc ccagatattt tattttggag atagacattc agcattggtt
     8641 tttttaatgt ttctttgcga acaatcaagc tatttcctat ccaaaaatac tacctcctcg
     8701 tcctgccaag ctcttagaat tggattattc ttagtatcca ttagcgtcca tttattgctc
     8761 gtgccagtcg attttgaatc attctattta attgtaacga agttttttag tttttcaact
     8821 gtcttttcac cgataccaga aatctcctgc aggtcttcca ctttctggaa ggatccgttt
     8881 tcttctctaa atcgaatgat ttcttgcgct ttgactgatc cgatcccagg aatctgctga
     8941 agttctgatg gagtggctgt attgatattt attttgggag gctcggaatc tgctgtaggc
     9001 tgttttcctt ccgaggatgc caactgatca agatgttctg gcagctcttc acctacagca
     9061 gggatatata ggacttgctg atcttctatc ttcgcagaaa agttgattcg atcgctatcc
     9121 gcttcttcta ataatccacc tgcacgcttt agtacatcat agacccgttc ttctgaagaa
     9181 aaggaataga taccaggctc cttcacactt cctttgatat cggtgtacat gacctttgga
     9241 cgagcatcct cttctacatc gtttccttca gctgatggat cttctccact ggaactaaac
     9301 gaagaaagta tttcttttgc agcagtctct tctgtttctc tataaaatag caaatttgat
     9361 aataaaaaac cacttgcgat acaaaagata agaactatca tagacatcca aatgatcaat
     9421 ttgggtgaag ctagtatttt cttgctgatt ttttctatca ttgttctttt ttcaaagaat
     9481 gaccagtaaa aatcattcat tactcctttc atttaaaatt gaagaaacag tgagctctgg
     9541 caaaatcgtc ccttcaacat ctaattacgt caattttttc ttttgttttt atttttatcg
     9601 tgaagatttt tgacgaaaaa gaggggttgt gacagaactc gtgtcacaac ccctctttaa
     9661 ttatttagtt tcatttcatt ttctctagat agtcaagcgc ttcttgtact gtttttactg
     9721 gtacgatttt cattgaggaa ttcaattttt tagcggcagc ttttgcttct tcgtagttgg
     9781 tttttatttc aggatattct ttcttaacat cgtctgaaat cgtatcatct ggtgcaaaga
     9841 aaatctctac gccagcttta tccgcacttg ccactttttt atcgattcct ccgattcggc
     9901 caacttctcc ttcactgttg atcgttccag ttcctgcaat ttgcttacca tgtcgcaagt
     9961 ttttaccagt gacttgttca tagatttcta aagtgaacat caatcccgct gatggcccac
    10021 cgatagagcc agaattaata cgaacatctg tatctgattc gatctcggta tggtcagtaa
    10081 gcgtgatacc tattcctgcc tttttatctg ttggtaactc gatcagatca ccagaagctg
    10141 ttttttcttc accattatgc aaataactga tttcgattgt ttgtccaact ttttggcttt
    10201 ttacataatc cataaatgct tctgtacttt gaaagctttt cccgtctact tttgttactg
    10261 tatctccaac tgaaagcttg ccaaagaaat tcgatgtttt ctctacactc atgacatata
    10321 cgcctttgaa ttccatttta taaggcttgt ctgcaagttt taacgcttgt tcgattgctg
    10381 cattttggga agaatccata taatactgtt gcatttgatt atattcttca tttgtcgccc
    10441 caccagtcaa ttcttcttca ctgatcaatt cttcaaaagg ggtcatttta gcttttaaag
    10501 ctaagaaacc tgtagcttgc ctgataccaa cagaggtaag agagaaagac ccagaacctt
    10561 tatcttcctt atcattgacc gtaattagtt ctttcagatc gatcgtggct cccggtgatt
    10621 ctacataata cggcaaggga actactacaa atccaatgat caacattcca aatatgataa
    10681 tcagtactgt tttcaaccat tgtttagctt tcatttgatt cacgctttcc agctaaagct
    10741 tcgttgatca ctggcggtaa gtagctgctc acatctccgc caaagtgcag tacttctttt
    10801 aaaatactgg aactcacgtg actatattct ggttttgcta ataagaagac agtttccaat
    10861 tcatcatata gatgatgatt catctgagca atctctcgtt catattcaaa gtctttgata
    10921 cttcgtatgc ctctgatcaa cgcatctgca ccaatctctt ttgctgtttc taccgtcaat
    10981 tgattttctt gatgcatgac ttttacatta ggaagatgag aaacagcttt tgtgatcaat
    11041 gtcattcttt cttctggggg aaacaaactt ttctttgaag tattgataaa aacaccaatt
    11101 actacctcat cgaataattt agccgcacgt tccaccgtat ctaagtgacc ttttgtaaaa
    11161 ggatcaaaac ttcctgggaa taatgcacgt ttcattgatt tccctctttt ctatagatag
    11221 ttacggccgt tatgccataa atctgttttc ttatttgatg gaatccagcc acagtttctg
    11281 gcaattcgac tgttttatcc gtttcgcata cgataactgc ggttttagaa aaaagtccca
    11341 gttcttccat tttatggatt tgatcgatta ttttttgttt tgcatatggc ggatcaagaa
    11401 acaagtagtc gaattgttgt ttctccgctg ctagccgttc gattgctttg tccgcatcaa
    11461 gttttattgt ggtaaatttc tctggttctt tcgtaatttc gatgttttct ttgatgattt
    11521 ttagcgcttg gtaatttttg tctacgcata ccgcatgact gcagcctcta gatactgctt
    11581 cgatagctaa accaccgctt cctgcaaaca gatccaatac tgtctctcca tcaaaatatg
    11641 gtccaatcat attgaatatg gattctttta ctttatctgt agttggtctg gtgttcgccc
    11701 ctgctaaact ttttaaacgg cgcccgccat aatttcctga tattatccgc atctactcac
    11761 tccttagaaa tggtactggt actagtatac caaaaaaata cttggacaat gatcaaaaat
    11821 cgatcgttat ccaagttatt ttttatttaa aaactgctca taagagattt ttaaagatgt
    11881 tcagctaatc ggatttctgt atttagctct tcaatttcga aaccttcgtc ttttaatact
    11941 gttttgtttc ctattttttc ggcaaaattc atttcgatat ccggtcggta agaacgttct
    12001 acgtttcgaa caaaatgtaa tttacggatt cgttcttctg tttcttccaa tgtctcttca
    12061 tttaagtaaa gcacgacata tttcattctt cttgagacat aatgcaacaa gccgaacttt
    12121 ttcagattct ttaattgttt taagctgtag acccatacga tcagcccgcg tcgttttgtt
    12181 atttcaaatt cactattgtc taattgcatg acaattacct ccgcatccaa agtgtttttt
    12241 cgtttcaaaa aacgggctac ctgcatctac tttgatttca ttagaaactt cttcggcaag
    12301 tacttggaca atttgatcca acagactttg cagctgtgtt tcactatatc ggaaagctgc
    12361 aacatgctca tccatatcaa gctgtctttt tgcttttcga acagctcgtt ttttttcacg
    12421 ataatcaggt gcatactcac catatgccgc tacattttca aatgcttctt tcttagacaa
    12481 gaacgcttct ttcaactggc aaacttttgc agattcttcc attgactgtt tatgttcaag
    12541 ataagaagcc atcgttttac tcttttttat catttcagcg acttggaggc attgatcctc
    12601 cagttgaaac agctcttcgt taacgatcac tgctatcact actttctccg ttttcttctt
    12661 gcaataagac taacatattt tctttagaaa aagcaatgcc tacagaatcc gttcccgggt
    12721 ccatgaaacg gtcatggttg taaggatcgc tgtaccaata taaaaacgaa aaagtagcgt
    12781 caatcgccgg atgaataaat agcccactag tattttctaa tttttgttta ctgattaact
    12841 tctccaattc atcattcgtc agtgagaatt tcactgtcgt ttgagcactg cctagcgatt
    12901 tttgccaatc agccatacgt tcctctgaca atatttcctc aggccttgtc aaaaaatcat
    12961 gtagtagcag ctgactgtgg ttttgaaagg caagtgtttg attgtatatg ggaagttcgt
    13021 cttctcttct aagcatattt aataaagtcg tactttttcg ttctttaacg cgattgattg
    13081 attcccaatc agccgattta tctgcttgat aatgtggaag gccttcacca aacacttgat
    13141 atggcaatag tttcaaaaga ctatctttat ctaaatacac aactgaaaat aggccgtttg
    13201 ttgtttgatc aaaaaataaa atagcaaaag tgtcgttatc aaaagcaatt agcggacgat
    13261 aattcatatc ctcttccatt agttcgattc caacttctgt atcatggtac tcaaaagtga
    13321 aattagtata gatagtagtg attttcgtaa gatcttgcat cgtcatccct aaataaaacg
    13381 gcgcgatatc agcaggccct gtttgcagta ctttaacagt tgtgactctg ctgtcttgga
    13441 tgttcgcttc aaatgtgaga tgactagctt tattttcata atatcttgtt tcaaaaccaa
    13501 atccgctagt taatcgatca taggcttggc caaaatcctt ttccagctga tcgatcggtt
    13561 ttccgatata agtagaaaaa ccagttgctt taagctcctg gtatttccac gttgtcattt
    13621 ggtttgatat ttgttgatca gagttttcct tcacttcaag ttttggcgga aacaagactg
    13681 gttccatgta gaatgccaca ataactaata gcagaatcag ccaaaacaaa cccagtcttt
    13741 tcatgtttcc ccacctttcg cttattattt ctcagtcact tcaatcaaaa ggtcaccaga
    13801 cgaaatagtg tctcctgaag acacatatac atgtgcgact tctccgtcaa aacgtgcttc
    13861 gatggccgtt tccattttca tcgcttcagt aatcatcaac gtatcacctt ttttaacgtt
    13921 atcgcctttt ttcactaata catccaaaac agaaccagac atcgttgctc caatttgttc
    13981 tcggtttgtc ggttcagctt tcaatcgggt ttggaccgta ctgatgatcg aattatcttt
    14041 gatagtgatt tcacgtcttt gtccattcaa attgaagaac agtacgcgat tcccttcgat
    14101 atcgggttct ccgatttcat ctaaacgaat gataagcgtt tttccgcgtt cgatttgtac
    14161 attgattgtt tcacccaaac gtataccttg gaaaaatgtt ggtgtgtcca gtaatgtaac
    14221 atcgccaaat tgattatatg ctttttgata gtccaaaaag acttgcggat acatcaaata
    14281 gcttaatact tcgtcttgtt ttggttcata gccaattttt tctgccaatt ctttttttac
    14341 ttcgttgaag tcgactgatt cagccaaaag acctggacgt tcattcaatg caggtctgcc
    14401 tttaagaata atcttttgca attttttagg aaatccgcca acgggttgcc ctagttctcc
    14461 ttggaagaaa gtcacaacag attcagggaa acttagtgtt tcgccttttt cataaatatc
    14521 ttcttctgta agatcatttt ggaccataaa tagtgccata tctccaacta ctttagatga
    14581 tggagttact ttaacgatat cgccaaacat caagttgaca gtatgataca tttgtttgat
    14641 ttcatcccag cgatgaccta atccgacagc ttttgcctgt tgctgtagat tcgaatactg
    14701 tcctcctggc atttcatgca tatatacttc tgtttcagga gcattcaagc cgttttcaaa
    14761 cggtttgtaa tacatgcgca catcttccca ataatggttt aatttctgtg cattttcaat
    14821 cgtgatttct ggcagccgtg gaccattgac cagtgcataa tacaagctgc tcatgctagg
    14881 ctgactagta ttcccactca ttgcactcat tgccacgtca acgatgtcta ctcctgcttt
    14941 tgaagcagca gaataagtga tgattccatt cccgctggta tcatgagtat gcagatggat
    15001 tggcagatct gtcgtttctt tcaattcact aatcaaacga taagctgcct gcggtttaag
    15061 cagcccagcc atatctttga ttgcaatgat atgcgcaccc agttgttcta gttcctttgc
    15121 catgtcttta taatactgaa cattgtattt tgcccgactt ggatcgttga tatcgccagt
    15181 atagcaaata gctgcctctg cgattttacc agtatcccgt acagcttgga tacttttttc
    15241 catttgtgga gtccagttca aactatcaaa gattcgaaat acatcgattc cttgcgctgc
    15301 tgcttctttg acaaattcca ccaatacatt gtcaggataa ttagaatacc caacggcatt
    15361 tgaacctctg aaaagcattt gcagcaacgt attaggcatt aaagaacgaa tttttctcaa
    15421 ccgttgccaa ggatcttcat tcaaaaagcg gtaagccaca tcaaatgttg ctccccccca
    15481 catctcactt gaaaagagtt caggaagacc ttctccagtc aaacgtgcaa tctgcttgaa
    15541 atcctttgtc cgaacacgag ttgctagcag actttgatgg gcgtctctaa atgtcgtatc
    15601 cgtcaacaac aaattttcct gtcctttgat ccattcgacc aaagcatcgg cacccttcgc
    15661 atctaataca ttcttagccg ttacgtaatc agttcttgtg attagatctt taggcatacg
    15721 tggttcttca taaaatggtt tttcgccact ttcgatacct ggaaaaccat tcacagtaat
    15781 ttcaccgata tatttcatcg ttttgtttcc gcggtctcgt agtcgaggaa attcaaataa
    15841 tgttgctgta ctatcaatga aggttgtttt tgcatttcct gattggaatt caggatgtgt
    15901 gatgacattg agcataaatg gaatgtttgt tttcactcca cggattctaa attctttcaa
    15961 gcaacgttcc attttctgaa tggctgtttc aaaggtagca ccatgcgtac atacttttac
    16021 tagcaatgaa tcaaaatacg gtgtcactac atagcccgcg taagcatttc cgacatccag
    16081 acgtacgcca aaaccaccag gtgaacgata cgtatcgatt ttccctgtgt ctggtaaaaa
    16141 gttattcaac gggtcttctg tagtgattcg gcattgaatg gctgaaccat ttaatttaat
    16201 tccttcttgc tgaggcaatc cgatttcttt atgcagatcc aaaccttgag caatcaacag
    16261 ttgcgttgtt acgatatcaa tatctgtaat catttctgtg atcgtatgtt ccacttggac
    16321 gcgagggttg acttcgataa agtaaaaatc atctccttca acaagaaatt ctactgtacc
    16381 tgcgttgaca tacccgacat atttcatcaa ttggacagct gcctggcaga ttttttgacg
    16441 ttgctgttca ttcatagaga cacaaggcgc tacttctact actttttggt gacggcgctg
    16501 aacagaacaa tctctttcaa aaagatggat gacgtttcca tgcgtatccc cgagaatctg
    16561 tacttcgata tgcttaggat tagcgatgta tttttcaaca tacacttcat cactaccaaa
    16621 ggctgctttt gcttcgcttt tcgcacgttc gtagccctct cttgcactct tttcatcgtg
    16681 agctacacgc atgccgcgtc cgccgccgcc tagagctgcc ttgatcataa tcggataacc
    16741 gtattgtttc gcaaactcca aggcatcgtc tatagaagcg attggtccat ctgtgcctgg
    16801 aatcgaagca atccccgctt cgatagctgc agcttttgct ttgattttat ctccaaaaat
    16861 atctaaatga tgcagtttgg gtccaacaaa aatgatgcct tcttcttcgc accgttgcgc
    16921 aaaattcaag ttttccgaca aaagaccgta acccggatga atagcgtcag ctccgcattc
    16981 tttcgcgata gaaatgatcc cttcaatatc tagatacgca tcaatcggtt tttttccttg
    17041 tcctactaaa tatgcttcat ccgctttgaa acggtgtacg gaatattcat cttcttctgc
    17101 atagattcca acagttttaa ttcctaattc tgtacaagca cgaaatacac gtacagcgat
    17161 ttcacccctg ttagcaacta aaactttttt catcgaacca ctcctacatc tttttttatt
    17221 ataaacctaa gctcttgcgt ttttcatccg cactgatgtt gaggacaaag ccgacacaaa
    17281 tcgaaagcat cagcaaactg gagcctcctt gacttaaaaa tgggaacgtg atccctgtca
    17341 aaggaataac gccagtaatt cctccaagat tgacaaatac ttggatcaaa aacattgcgc
    17401 cgatcccaat gcacaaaagc gaattgaacg gatcttttga acggatacct acaaggacaa
    17461 tgcgcacaat cataaagaat agcaacgcta atatcaaaat ccccataatc actcccagtt
    17521 cttccactac gattgcataa ataaaatcgg tttgtgcttc ttgtaaaaat ccttttttct
    17581 ggatactgtt acctaatccc ctgccaaata gtcctccgtt gaacatggca tagtagccgt
    17641 tgactagttg atgcccttta tttaattcgt ctgagaaagg attttggtaa atagcaaatc
    17701 gattatagat atactgtaaa cgtccgggta agatctttcc gtttgtgata ttgatcagcc
    17761 agatcgtaaa agtacttaga caaaagcctc ctactcctac aatcaaagta tacacataat
    17821 tgactccgct ggctaaaagc agaaccaaaa taatcaaaaa gataactgcc gcattaccaa
    17881 aatcgggaag aatagcgact aatgcagtaa gtccgataac cattgcaagt ggacgtttca
    17941 cagtcccaag aaaatctttt tgtacactgt tttgtctttt agataaagtc agagacaggt
    18001 accagataga aatgattttt aggtattcgg caggttggat cgagaatcct gcaatttgca
    18061 gccaaccttt cgccccattg atctccttac caaaaaacac aacaatcagc agcaaaatcg
    18121 tcaaaactgc gatggctgcc atgatcaaac gctggttttt caaaacatct gttttcattt
    18181 tataaagtag tgcgatagca atcaagctaa gcacccaaaa aatactctgg ttaatgactg
    18241 atgcggacgg attttgtccg ttttctaata atagatacga cgtagaacta tataccatga
    18301 tcaatcccaa tacacataaa attaaatagg gaatcaagat gctatagtct aatagatgcc
    18361 tttttttcac tttcttaggc aagtctattc ctccttgcca acttcttttt tatcataaat
    18421 ttcttggtaa acctcactat ataactgatt caattctcgt tctagctcac ttaacatcag
    18481 ctggccctcg ttctttccga ttactcctat acggacagca aaaccgactt gccgggaaaa
    18541 gccgtacatc tgtgtatcta ccacttcttc gaaggctttg cattgtgaga tacagagact
    18601 gttgttttga tttcggatta atcgtttgat ccgttctgct tcatcattta atacttggat
    18661 cgcaagtttt ttcgacaaag aatccatctg tgcttacctc ccattctaat tccttaatta
    18721 taacatattt gattttcata aggagcaaaa aaaacagatt taaccagttg tttttattca
    18781 tttttacgta ttcgttcttt ctacttatcc agaacgttag agtccgcatt ttttatccga
    18841 gataaaaagg cttcgccata tttatcaagc ttgctttggc caactccttt tatctgaagc
    18901 atctccaatc tgcttgatgg acgtattcgg ctcatttctt tcaatgtgga atctgaaaat
    18961 acgacatacg ggggaacgcc tgcttctgat gctagatccg ttcgcagttc ccgcagcact
    19021 tcaaatagtg tatcggtttc ttcatccgat agctgctgga ctttcttggg ttcctttcga
    19081 taaacagatt cttgtccttt caatacttgt atccctcttt ctgttacttt caaaacagga
    19141 tactcaccat tgatggtcag aagatagcca ttcgatatca aatattcaat taactgcatc
    19201 gtttcttttt gcgattggtt tttcatcagt ccataggtag ataaatgtcc aaaacctaaa
    19261 gcttgcagct tttgttcttt cgatcctgct aagactttca tcagcatttg tttaccatag
    19321 ttctcaccca tacgttttaa gcaagaaaga accatctgtg cttctgtcgt tacttctact
    19381 atttctctgt catctaaaca gttaccacaa cggttgcacg gactgcttgt ctcgccaaaa
    19441 tagtttagga tgtattgctg aagacaggac tcaatataca cgtattctgt catcgctttt
    19501 aatttttcat attctttttg cttttgccct tcttctcttt gggactgctg gataaaaaac
    19561 ttttggactt ggatatcttg aggagagaaa agcaggatgg cttcactgga aagaccatct
    19621 cttcccgccc gaccagcttc ctgatagtat gcttctagag agccaggaat ttgataatgg
    19681 ataacaaaac ggacattgct tttatttata cccataccaa atgcatttgt cgctacaatc
    19741 agctgcaagc gatcgtataa aaaggcttct tgcatttctg ttcgctcatt ctcatttaga
    19801 ccaccatgat atctacccac ggaaaaatcg aattttttaa gcaaatgata gagacggtct
    19861 acttcttttc tcgttgctgc ataaatgata ccagattgat ttttgttgat ctttaaatat
    19921 tcgattagat attgttcttt tttttgatct ttaattactt gaaatcgtag attttctcgt
    19981 tcaaaaccag tctgaatatg gttgttttct ggaattttta aaagtctttt tatgtcagca
    20041 gctacttgca cagtcgctgt agctgtcaaa gcaacaatta caggacgatt tggcagttga
    20101 tctagctggt tcgccatctg taaataactt gggcggaaat catgccccca ctgagagata
    20161 caatgtgcct cgtcaatagc gatcatacgg acgttccatc gcttcattgc ttggataaaa
    20221 tccggcatga taaaacgttc aggagcgata taaagtaaac ggtactgctg tctatcgatt
    20281 tcttggaagc gtctagctgt ttcgtaacca tctaacgagc tattgataaa agtggcgggt
    20341 atacctaatt gattcgccgc gtccacctga tccttcatca aagagattaa aggggacaca
    20401 accacagtaa gcccatctaa tagtaaagct ggaagctggt aacagattga tttcccactt
    20461 ccggtaggca tgattcctaa tacattttcc tgatcaatga ctttctggat aatttctttt
    20521 tgaccaggtc taaactcatc ataaccaaag tattgtttta ataagtcgtt catcttcccg
    20581 ctcctttctc ttgccatttt acttcgattt tcttcgaagg acaagttgat ctcctttcgt
    20641 gaaacattat cttattatga aacaaaaact ctctagaaat aagtttttta aaagtctttt
    20701 aaacgtcttt cggcgtattt tctgaattta tgtccattga caaagatgac ttctccacag
    20761 aaaagagttg gacaaaaaaa gaaaccagct attttttata acaagctggt ttctctcttc
    20821 atccttttac agattatttt ttctttttag atgctttttc acgagcattt ttctctaaaa
    20881 tttgttttct caagcgaatg ctttctggtg ttacttcgca atattcatca tcattcaaga
    20941 attccaaaga ttcttccaac gtcaattgtt ttggtttttt gatgacagat gtttggtctt
    21001 tagtagcaga ccgtacgttt gtcatttgtt ttgctttcgt gatgttgaca gtcaaatcat
    21061 tatcacggct gttttccccg ataatcattc cttcatatac ttctgtacct ggttctacga
    21121 agactgtacc gcgttcttcg atactcatga tagaataagt cgttgctttt cctgtatcga
    21181 tcgaaacaag cgccccttgg tgacgtccgc caatttgacc aggaagcatt ggcagatatt
    21241 ggtcaaaagt atggttcatg attccgtaac cacgtgtcat tgaaaggaat tcagttgaat
    21301 aaccaatcaa accgcgggca ggtgttaaga atgtcaaacg gatttgtccg tttcctgtat
    21361 ggaccatatc ttgcatttca cctttacgta agctcaagga ctcgataaca ctacccatat
    21421 attcttctgg tgtatcgatt tgcacgcgtt caaacggttc gcattttaca ccgtcgattt
    21481 ctttttcaat aacttctgga cgagatactt gcaattcgta tccctcacgg cgcatgtttt
    21541 caatcaagat agacaaatgc aattctccac gaccagaaac tgtccatgca tctggagagt
    21601 ttgttggttc aactcgtaag gatacatctg tttgaagttc tgccatcaaa cgttcttcaa
    21661 tcttacgagc ggttacgaat ttaccttctc gaccggcaaa tggtgaatta ttaactaaga
    21721 aagtcatttg caatgttggt tcgtcaatgt gaaggatagg taacgcatct tgatgatcca
    21781 caggagtaac tgtttcaccg acaaagatat cttccatacc tgagacagca atcaagtctc
    21841 cagcttttgc ttcttgaatc tctagacgtt ttagtccaaa gaaaccaaat aatttcgtta
    21901 cacggaattt cttaactgtt ccgtctaatt taattaatgc tacttggtct cctactttga
    21961 ttgttcctct aaatacacgt ccgattccga tacgacctac ataatcgttg tagtcaagta
    22021 atgaaacttg gaattgtaat ggttcgtcgc tgttatcgac aggagcaggg attttttcaa
    22081 taattgtatc aaaaataggt gccatggttg gttcttgatc tgctggatca tctgacaagc
    22141 ttgacgtacc atttagtgca gatgcataaa tcacaggaaa gtctaactga tcatcatctg
    22201 ctcctaattc gatgaatagt tcaagaactt catctacgac atgctcagga cgagcagaag
    22261 gtttgtcaat cttgtttacg acaacgattg gtgtgatatg ttgttccagt gcttttttca
    22321 atacaaaacg tgtttgaggc attgttcctt catacgcatc tacgaccaac acaacgccgt
    22381 caaccatttt catgatccgt tctacttcgc caccgaagtc cgcgtgtcct ggagtatcca
    22441 tgatgtttac gcgaatacct ttatagtcta cagctgtgtt tttggctaaa atcgtgatac
    22501 cacgttcttt ttctagcgca tttgagtcca tcgcacgttc ttgcaattgc gtatgtgcgt
    22561 ctaatgtatc tgattgtttt aatagttcat caactaatgt tgtttttccg tggtcaacgt
    22621 gggcaataat tgcgacgtta cggatatcgt ttctataatt caatgtaact aactcctttt
    22681 ttattggttc tttatgacag ggcaaatttt actcgactgc taattatagc agactttttt
    22741 catgaaatat agtaaaatgt tttcattttt tcgagaaaat taatcttttg ctcgttttta
    22801 tcatttttgt gaatgatttc tacctttttt tcagccttct tttttcaaaa ggtctgcgat
    22861 ttctgcatat gcacaagaag tccctgctaa atagtattcc cttttttcaa atgtcaaagg
    22921 agtaccaaaa agaccgcttg ttaccatacc aaactcttcc aataagacat ttcccgctgc
    22981 ataatcccaa gggctaaggt ttgaaatata agcaatgtgg tttcctttca agattgccac
    23041 tagttcaaga ccagcgcatc cgtacatacg tgtccccatg ctgctttccg caatttctcg
    23101 gattccatat ttattatgac cgaaaagata actattcaca ccaagtaagc cttccgataa
    23161 ggatttattt gctggtctag gcaatggctc atcattttta tacacgccta ttccttttcc
    23221 accccaaaaa agttcatttc tcatcacgtc ataaatgaaa ccaagctttc caacgccgga
    23281 ttcataaaca gcgagcataa tacaaaaatt ttccccttca atcacaaaat tcaaggtgcc
    23341 atcaatagga tcgatgataa agactcttcc atcgagatcc gataattgat tacacccttc
    23401 ttcttctgcc aatattttcg cctctggatc aaaggaatgg attttttcga ttagaaaatt
    23461 ctggatgttt cgatcgacat ttgttactag atccgttcga tttgattttt gcgagattgt
    23521 taatgtttgt tgagaagatt tgattttctc accagcttct tcaagccagg acttgatatg
    23581 atcaatcatc tgttccattt atctcacccg ttcattctca aagttttgtt tggatttttt
    23641 ttgcttgctg cactgtacga tataaagaat aaccggaagc tttttcaaat tcccgtccca
    23701 atcgtttttc ctcaccaatg gatttgacaa cagttttaaa ctgctgataa gtcttcaaaa
    23761 aagcttcatt gtttatccct ttttcattcg cacgttccag atcttcccac atcttcatta
    23821 caatgaccat ttcgtctgtt gtccaatcta aatcaagtgg atactgatat tcttgcatct
    23881 tcaactctcc tatttcatat tcctcttcag tgtagcatag gaagtggtaa taaaccaaga
    23941 agttgtcata tttttaagtt tacataataa tattattgat aattctaaaa aacaaaacgc
    24001 aactgatttt tcagttgcgt ttcgcctgta ttattcaaat caaatatgga ttggtaaacc
    24061 taatgctaat tccgaagcat ccatgacgat ttcgccaaga gatggatgag gatgaatcgt
    24121 caaggcgata tcttcagcat tcattcctga ttcgattgct aaagctaatt cagagaccat
    24181 atcacttgcg ccgactccac cgatttgcgc gccaataagc acgttatctt cgatcgtcgt
    24241 aactagacgg atgaatcctt ctgttttacc aagcgataat gcccgaccgt tccctgaaaa
    24301 tgggaattta tatgcttttg cttcgatacc agcatctttt gcttctttga tcgtcatacc
    24361 tacagaagcc aattcaggat cagtgaaggc tactgccggc attgctttat aatctacagc
    24421 tacttttttg ccagagattg cttcagcagc gatttttgct tcataactgg ctttgtgtgc
    24481 taatgccgcc cctggaacga tatcaccgat ggcatagatg ttaggtacgt tcgtacgtcc
    24541 ttgtttatcc accgtgatca atcctcgttc tcctacttcg acacccgctt gttctaagcc
    24601 catatcatct gtattcggac gacgacctac agtgaccatc acataatcag cagtaacgga
    24661 ttcttctttc ccgtccactg catatttcac tgtcacgctg tcaccgttat cgactgcttc
    24721 tttcgccatt gcattcgtta cgactgttac gccttttttc ttgaaatcat cttctactaa
    24781 ttttactaga tctttttcgt aagttggtaa aatttgtggt gagccttcaa gaatcgttac
    24841 ttctgctcct aagtttgcgt acgctccgcc caattcggca ccaatcacac cgccaccgat
    24901 gatcacaaat ttcttaggta cttctttcaa tgctaatccg ccagtagaat ctaacacacg
    24961 tcctccgaat ttaaatcctg gaatctcgat tggacgggaa cctgttgcga tgattgcgtt
    25021 attgaacgaa taagtttgag ctgaatctgg atgaatgacg cgtagtgtgt gatcatctac
    25081 gaagaacgct tctccttcga ttgtttcaac cttatgtttt tttaatagga aaccaacacc
    25141 agaagtcaac gtatggacaa ctttgttttc tttccattct tgtgtttttg caaaatctag
    25201 tttgacgttt tcactagtta ctccaaacat ttcagaatct agtgactctt gataatggtg
    25261 tcctgcagct atcagtgctt ttgaaggaat acagccgaca ttcaaacaaa caccgccaat
    25321 atactctctt tcaatgattg ctactttttg tcccatttca gcagcgcgga tggcagctac
    25381 atagccacca ggtcccgcac caattacaac tgtatctaat tctacagcaa aatctccaac
    25441 taccatttgt ttatcatcct tccatcatta atagctctgg atcagctaaa agacgtttga
    25501 tattattcat tgcttgttgc gcagttgctc catctacgat acggtgatcg aaacttaatg
    25561 acagtttcat cacacgaccg acaacgattt cgccttcagc atttacgatt ggttgttgtg
    25621 caatcgttcc tacacctaaa atagctactt ctggatagtt gatgactggt gtaaaccaac
    25681 cgccacctac agatccgata ttgctgatcg taattgttcc attgcgcata tcttctgcag
    25741 atagtttgcc gtcatgagct aattttgctt tctcattgat ttcgtctgca atagcaaaca
    25801 ttcctttgcg gtctgcatct ttcacatttg gtacatatag tccgtgatcc gtatcagttg
    25861 caataccgat attgtagtaa tgtttataaa cgatttcttg gtttgcatcg tcgatagatg
    25921 cattcaatac tgggtatttc ttaacagtcg ctgttaaagc tttcacgaca tatggcaaga
    25981 aagtaagttt cgtaccattt gcagcagcaa cttctttaaa tcgtttccga ttatcccata
    26041 gtttagacac ttctacttca tcatgcaatg tcacgtgtgg agctgtatgc ttactgttga
    26101 ccattgcttt agcgatagct ttacgtgttg gcgtcattgc tacacgttct tccaagtctc
    26161 ctaagttgga cttgaatggt tttgctggtg cagctggttt gctttctgct gcaggagctg
    26221 cttctttcgg tgctgccgct tctggcgctt ctgatttcgc tggtgcactg cttggaccac
    26281 ctgccaagaa gttttcgatg tcctcttttg ttactctgcc accttttcca gttgcagtta
    26341 cttgtgagat atctacatct ttttcacggg caaattgacg aacagatggc atagccaata
    26401 cacgtttatt tggatcggca gcttcaacga cacttgctga acctgatgtt tccacttttt
    26461 cttttggtgc ttcagctgat ggcgcactag ttgacggtgc actattgtgt ccaggagcat
    26521 cgatttctac taatacatcc ccaacatttg ctacagtgcc ttctggaaca atcacgtttt
    26581 taactgtccc tgtgacaggt gacggaattt cttctactga tttgtcattt tgaacttcta
    26641 gtaatgtatc atcttcattg atcgtatctc ctggtttgac gaaccatttg acgatttcac
    26701 cttctgc
//
LOCUS       DADOZM010000025        26408 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.025, whole genome shotgun sequence.
ACCESSION   DADOZM010000025 DADOZM010000000
VERSION     DADOZM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.025"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(130..825)
                     /locus_tag="J8I47_000909"
     CDS             complement(130..825)
                     /locus_tag="J8I47_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAZ6322365.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="J8I47_000910"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="J8I47_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAZ6322366.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="J8I47_000911"
     CDS             complement(2024..2215)
                     /locus_tag="J8I47_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAZ6322367.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="J8I47_000912"
     CDS             complement(2236..3126)
                     /locus_tag="J8I47_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAZ6322368.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="J8I47_000913"
     CDS             complement(3113..3880)
                     /locus_tag="J8I47_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ6322369.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="J8I47_000914"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="J8I47_000914"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAZ6322370.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="J8I47_000915"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="J8I47_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAZ6322371.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="J8I47_000916"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="J8I47_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAZ6322372.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="J8I47_000917"
     CDS             8496..8834
                     /locus_tag="J8I47_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322373.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="J8I47_000918"
     CDS             complement(9002..9760)
                     /locus_tag="J8I47_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAZ6322374.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="J8I47_000919"
     CDS             complement(9778..10596)
                     /locus_tag="J8I47_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAZ6322375.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="J8I47_000920"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="J8I47_000920"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAZ6322376.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="J8I47_000921"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="J8I47_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAZ6322377.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="J8I47_000922"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="J8I47_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAZ6322378.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="J8I47_000923"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="J8I47_000923"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAZ6322379.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="J8I47_000924"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="J8I47_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAZ6322380.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="J8I47_000925"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="J8I47_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAZ6322381.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="J8I47_000926"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="J8I47_000926"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAZ6322382.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="J8I47_000927"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="J8I47_000927"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAZ6322383.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="J8I47_000928"
     CDS             20638..20937
                     /locus_tag="J8I47_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAZ6322384.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="J8I47_000929"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="J8I47_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ6322385.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="J8I47_000930"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="J8I47_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAZ6322386.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="J8I47_000931"
     CDS             21904..23877
                     /locus_tag="J8I47_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAZ6322387.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="J8I47_000932"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="J8I47_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAZ6322388.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="J8I47_000933"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="J8I47_000933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAZ6322389.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8006 a         5212 c         5232 g         7958 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaa
//
LOCUS       DADOZM010000026        25775 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.026, whole genome shotgun sequence.
ACCESSION   DADOZM010000026 DADOZM010000000
VERSION     DADOZM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.026"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(388..1341)
                     /locus_tag="J8I47_000934"
     CDS             complement(388..1341)
                     /locus_tag="J8I47_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6322390.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1364..2119)
                     /locus_tag="J8I47_000935"
     CDS             complement(1364..2119)
                     /locus_tag="J8I47_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322391.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2116..3075)
                     /locus_tag="J8I47_000936"
     CDS             complement(2116..3075)
                     /locus_tag="J8I47_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAZ6322392.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3068..4018)
                     /locus_tag="J8I47_000937"
     CDS             complement(3068..4018)
                     /locus_tag="J8I47_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAZ6322393.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4168..4620
                     /locus_tag="J8I47_000938"
     CDS             4168..4620
                     /locus_tag="J8I47_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAZ6322394.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4709..6976)
                     /gene="gshAB"
                     /locus_tag="J8I47_000939"
     CDS             complement(4709..6976)
                     /gene="gshAB"
                     /locus_tag="J8I47_000939"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAZ6322395.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7163..8161)
                     /gene="ftsY"
                     /locus_tag="J8I47_000940"
     CDS             complement(7163..8161)
                     /gene="ftsY"
                     /locus_tag="J8I47_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAZ6322396.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8172..8981)
                     /locus_tag="J8I47_000941"
     CDS             complement(8172..8981)
                     /locus_tag="J8I47_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6322397.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8990..12571)
                     /gene="smc"
                     /locus_tag="J8I47_000942"
     CDS             complement(8990..12571)
                     /gene="smc"
                     /locus_tag="J8I47_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAZ6322398.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12591..13277)
                     /locus_tag="J8I47_000943"
     CDS             complement(12591..13277)
                     /locus_tag="J8I47_000943"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAZ6322399.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13625..15406)
                     /locus_tag="J8I47_000944"
     CDS             complement(13625..15406)
                     /locus_tag="J8I47_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6322400.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15432..16346)
                     /locus_tag="J8I47_000945"
     CDS             complement(15432..16346)
                     /locus_tag="J8I47_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322401.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16373..17335)
                     /locus_tag="J8I47_000946"
     CDS             complement(16373..17335)
                     /locus_tag="J8I47_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322402.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17335..18279)
                     /locus_tag="J8I47_000947"
     CDS             complement(17335..18279)
                     /locus_tag="J8I47_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322403.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18280..19284)
                     /locus_tag="J8I47_000948"
     CDS             complement(18280..19284)
                     /locus_tag="J8I47_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322404.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19552..19794)
                     /gene="acpP"
                     /locus_tag="J8I47_000949"
     CDS             complement(19552..19794)
                     /gene="acpP"
                     /locus_tag="J8I47_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAZ6322405.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19916..20917)
                     /gene="plsX"
                     /locus_tag="J8I47_000950"
     CDS             complement(19916..20917)
                     /gene="plsX"
                     /locus_tag="J8I47_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAZ6322406.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20966..23002)
                     /gene="recG"
                     /locus_tag="J8I47_000951"
     CDS             complement(20966..23002)
                     /gene="recG"
                     /locus_tag="J8I47_000951"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAZ6322407.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23119..24801)
                     /locus_tag="J8I47_000952"
     CDS             complement(23119..24801)
                     /locus_tag="J8I47_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAZ6322408.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24853..25215)
                     /locus_tag="J8I47_000953"
     CDS             complement(24853..25215)
                     /locus_tag="J8I47_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ6322409.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25485..25673
                     /locus_tag="J8I47_000954"
     CDS             25485..25673
                     /locus_tag="J8I47_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAZ6322410.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7041 a         5639 c         4540 g         8555 t
ORIGIN      
        1 ctagtctagt ggacttattg ccgagaagca gcagatggaa catcgtttaa agaagagatc
       61 ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg tacctttgat
      121 tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt tttgccactc
      181 cccattctag aatcaacccg tatgatgtag ataatgaatt cacgttgaaa aaagatttct
      241 caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg tttttgataa
      301 cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg ttacatagta
      361 tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat cttcgatcat
      421 cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt caagggagat
      481 gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag aaatatcatc
      541 tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga tatccggatt
      601 tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt cgatttgatc
      661 atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac cagatccgta
      721 ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag tagcttctgc
      781 tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt tagcttcttc
      841 ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt cccatggatt
      901 tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt tttgaaaatc
      961 tctttgtcgg ccagaaatga tgattagatc tggctgaagc tgattaattt tttctaagtc
     1021 tggttctttt attccacctg ctgattctac ttttttatag gatgaaagat actcaggaag
     1081 gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca tcgtatctaa
     1141 agaaccattg tcgaatacga cgactttttc tggattattt ggcacctcga tttggtctcc
     1201 attactatct gttatcgtca atgtagtttc agtttgtgtg ctgctttttt ttgtgtctgc
     1261 ctgactggtg ttgttgctat ttccacatgc tgctaaacca aatgatgcta gaatcaagat
     1321 acctactact aattttttca tctcttctct cctaatgtta gttttagaaa taaagacaga
     1381 atctgcggcc ttcaatctca caaatccgta tgtccatttc gtataattca tccaatacat
     1441 ctttttttat catttcttgg gtcgttcctt gcgtaaataa ctgtccatct ttcattgcaa
     1501 caatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata acaattgttt
     1561 tcccgatttc ttgtactaaa ttttttaaaa tctgcatgat ttgaaccgca aaattcatat
     1621 ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct agcaccattg
     1681 caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga tccgctattt
     1741 cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt gtcgtcaatt
     1801 ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg atttggacat
     1861 ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg gatttccaag
     1921 cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc aaacgactga
     1981 tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca gtcaatttct
     2041 tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac agtttattga
     2101 ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg tgacaaaata
     2161 aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga aaatctgctc
     2221 tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc cgataaatag
     2281 ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa taaaccctaa
     2341 aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga cacaacataa
     2401 aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt catttccgag
     2461 atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc cgataatgat
     2521 caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa ataatctgcc
     2581 ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac tgacactacc
     2641 aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg ctctcatttt
     2701 tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa cgttcaataa
     2761 gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga agaaaaggac
     2821 cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca aaaactggtt
     2881 atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga caaggataaa
     2941 tgcaaaaatt ttcttactcc tcaatgcaca tgcaaaatgc cagtttccat atgtatggat
     3001 cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa aaatagatgg
     3061 ctttttttta agcatatcgt cgtcccctta tcagaagata aaggaagata gccccaccga
     3121 tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg cgagcgacaa
     3181 gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt gttttgtgca
     3241 cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata aaaggaatgc
     3301 ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg cctaaaaact
     3361 gcatcttctg gtaatctatt cccagattta ctgcaaacga ttcgcccaga ccaatgatcg
     3421 taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca tacaataact
     3481 cataatttcc tcgtatcaca gtcgcaaaat tcccttgaag ccatgaagtc atattttgta
     3541 ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc cctaacatga
     3601 ctccaatcaa cggaagcatc aattgatttt tctggaggaa caatcgcgta agggataaaa
     3661 aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca atcaaggatg
     3721 caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc atcgtgcccg
     3781 cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac ccacaaatac
     3841 tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc actagcaaga
     3901 tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc gatactccga
     3961 tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg tagcgcatcc
     4021 aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc gttgaacaaa
     4081 agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat cgtaagaaag
     4141 acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag ccatttggat
     4201 aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata tttagggaca
     4261 ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc aaaaaacaaa
     4321 gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt gaatggggct
     4381 ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa tagccgtttc
     4441 actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct cttaacagca
     4501 aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga agaaaactct
     4561 tcgccagctg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg tttattttaa
     4621 aacgaaaaaa cacataaatt tttacatgca aaaaaagctt ttgatcagat tgacatcaaa
     4681 taggacaatc tgtcaaaagc tttttctttt aaaatacttc aggatataac aatttcaata
     4741 cattcatcgt caatcttctg ccttttcctg caaaaggata aacatgcata tgcatggctg
     4801 gattgaaatt tgcttcgatg atcccatatg cttttttatc agttgtagga tcgatttctt
     4861 tatctggtat aatcaaatcg ataccactaa tcttagctcc tagtgcttct accgcagaaa
     4921 cggcgatttt tttgtaagct tcagaaaatt catcagtcat atcgatcgaa tcaccacctg
     4981 tactgatatt tgagttctcc cttagataaa caatttgatt ggcttgggga actgattcga
     5041 tcgtcaaccc ttgttctttc aacatcaatt gttctaattc acctaattgg atcaattcta
     5101 aaggtgcacg atgattcgtt ccacgcaaag ggtcactatt tttttcttca acaagttctt
     5161 tcactgttcg gatactgtca ccaataacat tagcagggac tcgcaacatg atggcttgga
     5221 cttctccgtc gatcacaaag aaacgatatt ccgttcccgg cataaattct tctactaaaa
     5281 ccgaactgtc ttcacgaaaa gcaatggcta aacctgcttg ataatcttcc agactagctc
     5341 cttccttgaa aatcgtgata cctaacccgt aattggttga tttaggtttg atcacaaatg
     5401 cctgctcggc aaatctcgga taagctttta ccgcttcttc catggatgaa aattctgcgc
     5461 ctcctggtac ccggaaccca gcttctttta agactttttt cgttactgtt ttgttttcca
     5521 tgataagtgg gacgatatag ctgtccttac ttgtcatgtt tgcatttttc acatattcta
     5581 catggtcttg gaattgcaga cgcaaaaatt gatccgactc atctaatact ttgacttgga
     5641 cacctttttg gatggcatcg aacataaata tctgagtaga aagttccatt tcgcgaaaac
     5701 cggccaactg atagggacgt tcatggcttt ctttataata ttgatgtccc aatgccacag
     5761 caacttcttg ctgactcgat agctgtgctt cttgatagag tttcgctgct aacgtttgtt
     5821 caggatgatc catttgagtc aatgcttcct ctactaaata aagagaatcc aaccaatcca
     5881 attcaaccag catttgcttc attccttcaa gaatttctct accttcttga tgaaattctg
     5941 tttgatctga tggttgttct agtgctactt tgttatttcg agtcgtacct tctgctaccc
     6001 attggtcaca gtcttctttt tcttctgtcc ataacatgta catcaggaat aattgtaaga
     6061 accgaacttg ctctggattg atacccaatc ttgcataagg attcaaatcg atattccgca
     6121 attcaatata acgaacacct gttgttgcta aatctgcgac tttctttccg cctcggaaac
     6181 gaagcggtgt ataaaattct ttctcctccg aaagtttccc ttcttcgatc attcgctcga
     6241 tatctgctaa atactgtttc atagaagcat aggaaacttt cacatttgga tgattcgtat
     6301 agcccagcgc actgttgcga atactgcgaa caggttcttg cggatgtgat tcatctaaaa
     6361 agtagttctt ctcactcatt ggtgaagcac cgaataaata agtgatcatc cagcgatagc
     6421 gcataaagtt tctcgctgtt ttcaaataaa gttctgtttt gaattcggaa aaatcttgaa
     6481 attcttcctg atggctgaac aatgttctta gcaactcatc gccaaattca aaattgaaat
     6541 gaataccgct taccatctgt tttcgcttcc cgtattcttt tgctaaataa cgtcgataca
     6601 agacatcttc gaaatttttt aattttgcaa taatgatctc ttcgtctttt tccggtaagg
     6661 caggtggcat acttaatggc cagatcatct cttctttcgg tatagaacga gcagtcacat
     6721 cataaacagc agcgagatac tgaaataatt cgggaataga atcggtaaca ggggtgatca
     6781 tctctgtttg tgtttcgcta aaatctgttt ggatataggg gtgataggat cgatcgccaa
     6841 agattgctgg atgatcggtt tttgctagat ttcctgcaag atcaacccgc tgtccttccc
     6901 gttctatacc aaatcgagct tggtcgataa aagggcgcgc attgacatgc aataataatt
     6961 gcttaaaatt catcattaag tgacctccaa ttcatatgaa tctaattata atcaaaaagt
     7021 ctaagaaaaa gaacgaaatt gtttgagaac cgcttacaaa taagtataaa aatgaaggca
     7081 tcaaaaacac agctatgtta aaaacaatca ttcaaacaag gagctgcaac ttttgtcaca
     7141 gctcctgctt tttagacatg ggttattctt cttttagtaa tcctttgaac aatccaacga
     7201 caaaatcatt tggatcaaac ggctccaagt catcgattcc ttcaccaaga ccgaccaatt
     7261 tcactggtaa atgcaattca ttacggatag caagtacgat accgcccttt gctgtaccat
     7321 ctaattttgt caaaacaaga ccggtcacat ctgttgtttc tttgaattgt tttgcttgaa
     7381 ccatcgcgtt ttgaccagtg gttgcatcta atacaagaag tgtttcttgg ggtgcttctg
     7441 gtaattcccg ctggattact cgttttattt tttctaattc gttcatcaag ttcactttat
     7501 tttgcagtcg gcctgcagta tcaactagta aaacatctgc ctgctctgtc tttgctcttt
     7561 ccaaagcatc gaatacgaca gctgctggat cgcctcctgc attgccacga acaacttcaa
     7621 caccagcgcg ttcgccccag acaactaact ggtcgattgc tccagcgcgg aaggtatctg
     7681 ctgctgccat caatactttt ttaccttcgt ttttgaactg atgggcaagt ttaccaatac
     7741 ttgttgtttt ccctacacca ttaacaccga caaacaaaat aactgttagt ccgtcttctt
     7801 ggatatttag ttcatttact tcattgattc cttccgcttc atagagatcc acaagtttct
     7861 cgatgatcgc attttggact tctgccgatt ttttcgcatt tctgagtttc acttcttgac
     7921 gtaatgcttc ggtaattcgt atcgctgttt caaaacctac atctgcaccg ataagtgttt
     7981 cttctaattc ttcaaaaaag tcttcatcga ctgttcgaaa attggcaaac aactcattca
     8041 tccgttcacc aaatgtttta cgcgtctttt ctaaaccctt gtcgtacttc tcctgtactt
     8101 caacttcttc tgttttctct tcgccagtaa aagcacgctt gattttatca aaaaatccca
     8161 tttgactccc cctactctaa tacaaatttt tggatcactt tggctacgcc attttcttca
     8221 ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt ctccatcgct
     8281 acgcctagtc cagcatattg gatcatcggt aaatcatttt cctcatcacc gatcgccatg
     8341 atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata agcttttgta
     8401 acaccttttg gcataaattc caaaagcata cttcttgttt tgatgatctc atacatttca
     8461 tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc ataagcgatt
     8521 actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg ttggaattgc
     8581 agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg cagctgtaaa
     8641 accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag tttgtataag
     8701 cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt atcatttttt
     8761 tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag tcctaattct
     8821 tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat cttgacccct
     8881 tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt ttgttcattt
     8941 aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc tacactttcg
     9001 tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg atttttgata
     9061 caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc gttcctttgc
     9121 ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa cgaccaaaac
     9181 gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa aacggaacag
     9241 gacagacacg gataatggaa aagagcaacg cgatggctgt aagtgctcgt tctcctccag
     9301 aaagcaaact tagactttgc agttttttgc caggaggttg tacttcaatc tcaatacctg
     9361 ttttcagcaa atcagttggt tccgtcagta ctaattcagc tttaccgccg ccaaacatat
     9421 ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg aatcttgtcc
     9481 gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct gctaacagat
     9541 catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat tgttcgattg
     9601 aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa acctttgttc
     9661 tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca aatgtcagct
     9721 gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct cggtcttttt
     9781 ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta cgctctgcca
     9841 agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct ttggcttttg
     9901 ccaactcggc ttttaatgtt tctcgctgag cagataattc gttaatttgt ttttccaaac
     9961 tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac tgcttttcta
    10021 tcgcttcttg acgttcggtt gcctctgctt tttggactcg ggcaccgcgg agctggatct
    10081 gcagatgatt aaactgctct ttcaagacag caagatctgc ttgttcttgt gctttttgac
    10141 tttgtacttg tgctcttctg gcttccatct ggtcgctttc ttgacttaac gatttgattt
    10201 cttcgtcgat tttttgtcgt tgcgattcaa gatctgtttg tttgtctttc aattcgttcg
    10261 tttgcttttc gtaatcttcg ataaactgct gcagttcacg tgtctcaaaa gttgagattt
    10321 gcttttcttt ttcaaaacgt tcaagttcat ttatgatgtt ttgtagctga tttgtcgctt
    10381 cttgttcttc aaaacgtaat tgttcgcctc gtgtacgcaa aacttcttgt tcttcggcta
    10441 aacgagctgt ttcttgttgc agctcctgaa cttttttttc ttgtgcttgg agttgtttat
    10501 ctgcttcttc aaattcacta gtcaactgtt tcaattcttg attttgtaca aacaaactgc
    10561 cttggtttcc tcgtttattc gctccacctg tcatggatcc tccggcattc atcacatcac
    10621 cttctaagga aaccactcga tattgatagc ggatcgtttg cgcgattgca ttggcactag
    10681 ttaagtcttt ggctaaaagg atcgttccta gcaggttttg aaccacagtt tggatttgat
    10741 cgggataact gacttgttca ctggcaatcc ctataaaccc ttctacagct gctgcttgtg
    10801 tcaatatatg agccggtaat tgtcttggtt tgattgtcgt cagtggaagg aatgttgctc
    10861 ttcctcctct ttgctgtttc aaatacgtga tggcttgccg agcatctttt tcattttcaa
    10921 cgatgacatg ctgggctgcc ccacctaaag ctgtttcgat agctaacgtg aaatctgcag
    10981 gaacatcgat cagttcagcc actgctccga caatacccga aagctgctgt ttatgctgca
    11041 agaccaatcg aaccccttgg taaaaaccaa aatagttttc ttggatttct tgaaggcttt
    11101 tcttacgagc acgtagctgc tggacttgat tcattaattg atacatttta ggctgttctt
    11161 ttgctaatgc ttcttggaac tttttcccgt tttgttggat catttctaac ttctcttttg
    11221 tttcagttaa agatgatttt agctgcgcct gcttttgtgt caattcttct ttttgtaaga
    11281 tcaatcggtc tactgatgct tctacttccg attgcttggc taacgtctgc ttgctttttg
    11341 ctgtttcttg tatatattga cgttctagat acttcagttc gtttcctaca gctgcttgtt
    11401 cttgcatcaa atcaacatat tgatctctta attctgccaa taattctttc gttgattttt
    11461 ggtatttttc taattcttgc tgtgttttta tgagatttgc ttcagctttt tggatctctg
    11521 tttctttttc tgctgctgct ttcatcaagc tttcttgaag cttttcaaaa tgtttaacct
    11581 ttttttgtgc ttctgctaat gaagtttgat attcttgcga gcttttttgt gtatgctttg
    11641 tccgctcctg caaaacatct ttttgtcctt ctgtctgctt cagtttttcc gaaagatcta
    11701 ataatacttg ctgatttttt tcgatcagac gatcggcttg cgcattttct ttacgctgct
    11761 tcgccaaaat ggattcttgt tcttggatcg actcagatag tttgcctaat tctagattga
    11821 atttcgcaag ctgtgcttgt ttgttgtccc aatctttttt tgctgtcttg atttcagcaa
    11881 ccatcaaaga gacatcggtt tgagtcagtg tttcttttag ccgtaaaaac tcctttgctg
    11941 cctcactctg ggcggccaaa ggagttaact gttcttccaa ttcgtggatg atgtcttgga
    12001 cgcggctgag gttgtcttct gtttcaaaaa gcttctgttc tgctttcttt ttccgctgct
    12061 tgtatttcaa gacaccagct gcttcttcaa agatcccacg acggtcttcc ggtttactac
    12121 tgaaaatcgc ttctactttt ccttgagaaa tgattgagaa cgattctttt cctaatccag
    12181 aatctaagaa caattcttgg atatctttta atctgcagct ttgtttattg atgaaaaact
    12241 cgctttcccc agtccgtcga taacggcggg ttacactgat ctcattgtac tccaatggca
    12301 gataatgatc actattgtct aaaatcactg tgacttctgc aatattcagc tgcttacggg
    12361 tatcggaacc ggcaaagatg atatccggca tttttccgcc tcgcagactt ttagcagatt
    12421 gttctcctaa tacccagcgg atagcctctg taatattgct ttttccgctg ccatttggtc
    12481 caaccactgc tgtgacgcta ttttcaaaat caatgactgt cttgtctgca aatgatttaa
    12541 aaccggctat ttcaattcgt ttcaaataca aagctttccc tcacttactt ttacagtgct
    12601 gccaatgcat tttccgccgc agcttgttct gctaattttt tcgatttccc ttgtccagtg
    12661 ccaatcaatt gatcatctac ataaacttcg atccaaaata ctcgttcatg agctggacct
    12721 tcttcattga tcaatcggta ttcgattgat acatcacctg atttttgcaa gacttcttgt
    12781 agttttgttt tatgatccat ctcatgtgaa aaagcacctg ctttgacttt aggaaaaatc
    12841 actttttcga tgaatgcatg ggtcgtgtcg aatccctggt caagatataa cgcgcctaaa
    12901 aaagcttcga ataggtcgca aagcaatgct ggtcgcgtac gtcctcctga attctcttct
    12961 cccttgccca acaaaacata ttgatcaaag tgacattctt tggcaaattt cgacaagctg
    13021 tcttcgcgaa cgatcgcagc tcgtgttttt gtcaattttc cttctggcaa atgaggatat
    13081 aaacggaata aatactgaga aaccatcagt tctaaaacgg catctcctaa aaattcaagt
    13141 cgctcattat ccgataattg cagattgcga tgctcattca cataggatga atgggtaaaa
    13201 gcttgttcaa gcaaatttag atcgtgaaaa acgatgttat agttttcttt tagttctttt
    13261 gtcagctggt tgtccatttt ccacattcct tttatggata ttttttcgat acttccttat
    13321 tatactggtt ttttgtcaaa tttgaaatgg ttcggtcaaa aggtaagtaa agctttaata
    13381 atcctagtga ttgacgagaa gttgtgaaaa attcgtttag tttcgagtat gaagaaaaaa
    13441 ttttgtgaaa atggctttcc atatttttgc ataaatttga cttaataccg agaaactgga
    13501 ctttgaacac cgtttattgg agaagtcatg aaatcagcgc cttgacctga atattagaga
    13561 aataaacaga aaaatatcaa aaaaccgaaa aaggacatcg ccctttttcg gtcattttta
    13621 gtgattatga acgtgaatct gctgtaacag aaacatttgc ccatgggttt ggtacatcat
    13681 aagaccaatc ccagtctttg acacggtcgt tgactggcaa tacttcatta cggaacaacg
    13741 ttggaatcgc aaatgcttct tcaaatgcat attgttgcca tttttgatat gcttcttttt
    13801 ggtactcttc atcaaatgct ttgcttgaac tgatatcgtt gatcaatttt gtattttctt
    13861 ctgattcata acgagtgtag ttgaacgcag catttggtcc ccatagtcca cttggatttg
    13921 gatcagttcc tgtattccaa gcagcttggt aaacatcaat ttcaggatca tcgttttcta
    13981 atttatcata gaaagaattg aattcgatca gacgacctgt tgtgtattgg acatccaaac
    14041 caatttcttt ccattgttgg atatagtaat ctgccaatgg ttgcgctgtt tccccaccgg
    14101 acatcgaagc aaagttgatt gttagtttat ttccatcttt gtcttctctt aaaccatcgt
    14161 catccacatc tttgtagcca gcatcatcta atagtttttt cgctttgtct agatcttgtg
    14221 tgaatccttt catctcatca tcatggtatg actggaatac tggaggaatc aatgaagtcg
    14281 cattgcttct caaacctgca tagaaacgtt cacctactgc attattatct aatgcatagc
    14341 ccattgcttg acgtaatgat ttatcagcca tttttgaatc tggattgtat tccacactct
    14401 ttgtatctgc attccattta cctaacttga atcctagata agtataagaa gtttgttcac
    14461 ggcctaagtt tgtatagcca gcaatttctt tataggtatc gtaagtatct gttggcattt
    14521 ggaaaatcat atcatataat ttagagttca atgcttcagt tgcacttgcc atcggtacag
    14581 atttcatcgt gatctttttc aatttaggcg taccattcca gtagtgctcg tttggcaagt
    14641 attctactga ttctcctgca acgatattac tcatgtaata agggccaaaa gtcactggat
    14701 ttttacgtac ttgatcgctt gattccatat ctttgattgc tacaccttct aacgtatgtt
    14761 ttggtgctgc atatgaccag ataccaccac ccgattgaag catgcttggg tttacttctt
    14821 tatattcgat ttcaactgtt ttgtcgtcta tttttttgat tccggagatt gtgtcggcac
    14881 ttccatcatg atattcttcc atacctacga tgttacggaa agtgtcgtca taacggatac
    14941 cagtgtaatc tttattccca atgatttcgt aagggaaaat cacatcgtcc gccgttacat
    15001 cttcaccatc tgaccatttg acgttgtctt ttactttgat tgtcgctttt ttcgtatctt
    15061 catctagatc aagcgttgca gctccgtcgt ctgtgattct gaaatcgtca tcgtttgcaa
    15121 atagcggttc atgagaagga gccataaaag ctgcatcata tgaatcttgg taaaactccc
    15181 actggaacaa tcctttaaac tgactgtccg ttgcgaccgc agcttctaat tcgccgtctt
    15241 cgattgcatc atcattattt tttgttgcaa ttggaaaagt taattcctct ttgttattgc
    15301 cgttgcctcc agagttgttt ccagaggtat ctgtacctcc gcccccgcat gctgcaagaa
    15361 cgacagcaga catcagcgct gcaaatccaa aaatcgtttt cttcattcct atttccccct
    15421 tcgcttctat actagcctaa ccgttggcgc gcatctgcag aacgcttgaa tgcttgtcca
    15481 acataattga tactcaacat caaaactaaa ataaggattg atgcgggtaa ccagatccat
    15541 tgccgattca ctaagacttc tccgttactt gcataaccga tcaatgtacc taaactaggt
    15601 acatttgccg gcaagccgaa acctaagaaa gtcaaagttg tctcgatacc gatgtttgcc
    15661 gcaaagttca tcgtcaaatt agtaatgatt aatgaactta gattcggcat gatttcgcgg
    15721 aacatgattt taaaatcatt ggttcccatt gtttttgagg cactgacata atctcttctt
    15781 gcttcagata atgtcttgct tcggaataat cgggctttag ctacccaata aaatgcactc
    15841 atgatgaata taaaggacca gacattgtat ctggaaataa tcgatacgaa gacgataatg
    15901 atcagcatga tcggtagaat catgataaaa tcaacgatcc gcatcaagat gttgtcaatg
    15961 atccctccgt agtagccaga gatgatccct aatccaaccc cgatgatcga agtaaggatc
    16021 gtgatcgcaa atccgatcgt aacagagttt ctagcaccta tgatcaattg tcctaataca
    16081 tcacgtccgc cttcatctgc tcccagaatg aacttttgtc cttgtgtcga aactgttcct
    16141 ggtgcagcgt atttgtcgaa gatactaaca tacatcactt tgtcttgatc ggtcagcatc
    16201 gcaccgataa agatcactaa taataaaacc actagcaaac ctaatgaaaa aatagcaatt
    16261 ttatcttttt taaattctcg agcaatcatt cgtatcccca taggaggaat actttcttga
    16321 actgctgtat tgtttttttc actcatcgat ccattctcct ttctgattgg ttttattgaa
    16381 tccgaatacg cgggtcaacg atactcatga tgatatctga aagtaatgtc cccactagcg
    16441 tcgcgattcc taaaatcaat actaaggatg tgatcacggt atagtcccgt tggccgatgg
    16501 aatcaataaa tagttttccg actcccggat aaccaaaaat cttttcgata actacagaac
    16561 cagcaatcaa cgaagtgatt tcatacccca tttgagaagc catcggcaac gctgcatttc
    16621 ggaaaatatg tcgactgaat actttattcg tcggtacccc tttggaacgg gctgttctga
    16681 caaaatcgag cgatttcgaa tcgattactt cacttcgcag atattggatt gtgacggcag
    16741 tcgccaaaaa tgcttgggta attgatggta acagaatata tttcaaacgg tctatccatt
    16801 gagtgataaa tccaccttgc aaacctagtg tttgtgatcc acttgttggg aaccagccta
    16861 aacgataacc aaaaacaaat agcatgatca agccgaagat aaataaagga acagcaaaac
    16921 taaagaagtt gtagattacg acaatcttat ccaagattga attttgatac cggccagcaa
    16981 gcatccccag aggcacagca atcaaataag taaggacaac tgtcaagatc gatagatata
    17041 aagtgttgat gatccgttcg ccgatcaagt cagcaacaga gcgtttgtat aagaaacttt
    17101 gaccaaaatc gccttgcaaa gcatttccga tccaacggaa gtactggaca taccatgggt
    17161 cattcaagcc agctgcttgt cgcatctgtt cgatcactgc cggatcctga tttggattga
    17221 tcaaaccggt aaatggatcc cccggcatcg cttgggctaa taagaagatc agaacactta
    17281 aaataataac ttgcgggatc atcaataaaa cacgtcgcag aatcgttttc cacattagct
    17341 tgcacctcca tctttcaacg ccactttgtg tgtagttgtg atcgtacgca aatcgtagac
    17401 tcgaccagtc gcatcatagt attctttttg gttttggata tactcttgtt ctactcttcg
    17461 tcgattttct ttgtggcttt ccctattttc tacatcgatt tgcggaatag ctgaaagcag
    17521 acgtttcgta tagatatgtc gcgggtcatt ataaatgtct tctcttgtac cgatttctac
    17581 aaatcgacct ttgtacataa ttgcgatatt gtcgcacatg tgttttacta cacctaaatc
    17641 atgtgaaatg aataaatagc tgaggccgta ttcttgctgg attcgtttca tgaaattcaa
    17701 tacttgcgct tgaacagata gatccagagc agatacaggt tcatcggcta cgatcagttt
    17761 tggattggta gcaacagcac gggctactcc tagacgttga cgttgtccgc ctgaaaattc
    17821 atgaggatat ttatatagcg catcttccgg catcccaacg atatctagca gtccttttac
    17881 ctttttcttt tcttcttgat cgcttaaccg ttcaaaattt cggatgggtt cagcaatgat
    17941 atccaacact cgctttttag gattcaaact ggacattgaa tcttggaaga tcatctgcac
    18001 atccttgtta taacccattt ttttgcggtt gcttcgcttc gttacatctt ttccttcata
    18061 catgatttgt ccggctgtta ccttttctaa tcccacaacg gcttttcccg tagtagattt
    18121 tccagagcca gactctccca ctaaaccata tgtctttcct ttttcgataa taaaatccac
    18181 accatctaca gcatatacat gatccgttac tcgattgaag aatccactgc gaatagggta
    18241 atgaactttc aggtctttga ttgtgattaa ttctgtcatc tacacctctc ctttttcgtc
    18301 tctaaagtgg aaatgcttat agcaggagca acgtaccaag tggttcggtg cgacttcatg
    18361 aaggataggc tcttcttcat gatcgcttgc tggtatccat ggaatacgtg gtgcaaaccg
    18421 acaaccttta cgaggcatgt ttttcagaga aggaacgaca ccttcgatca catggagttc
    18481 tgcatcatga tttccttctt gtggtataga gttcagtaaa gagcgtgtat aaggatgctt
    18541 aggatgatca aacagttctt ttacacctgc tacttcaacg aactgtccgc catacatcac
    18601 tgccactcga tcagccattt ctgctacgac tcctaagtca tgagtgatca agatgatccc
    18661 tgtttttgtt tcttcctgaa ggtctttcag taaatcaaga atctgtgctt ggatggttac
    18721 atctagggct gtcgtcggtt catctgcaat gacgattgga ggtttgcagg cgattgcgat
    18781 tgcgatgatg acacgttgtc tcataccgcc tgatagttca tggggatatt ggcgtccaac
    18841 tctcgcagga ttaggaatcc ctacttgttc tagcaattct agtgcacgag cttgacgctg
    18901 ttctgcattc atctttgtgt gataggaaag tccttctttg atctggtctt cgattctcat
    18961 gagaggattt aatgcagaca acggatcttg gaaaatcatt ccgatatcat ttccgcggat
    19021 tttattatag agcgtttcgt tcaggtcgat taaattcaga tcgttatata aaatctcacc
    19081 tgtaattttt gtgttattag gatcaagtaa tcccataatc gtagtagcca aggtactttt
    19141 cccgcaccca gattccccaa cgattgctaa aatctcgttt ttgtctaaag taaatgaaac
    19201 gtcatctact gcatcgtaat agtcatcttt gagtcggaat cctgtatgta ggttctgaac
    19261 ttctaataat tggttatcgg acaatcgaat acccctctct gcttcctaaa tggaagtttt
    19321 tcttaataaa aaaggacatt acacagaata aatcgtttta taacaaaggt tattcttatc
    19381 tttgtgtaat gaattattaa taattatata gaaaaccgct ttactttgca acataatttc
    19441 tgtttatcgc cagaattatc agaaaaatga cttttacgtg tcaaaaagat taaaaaaaga
    19501 aggataaaaa gctgcatgca gctttttatc cttctttgct tttatttgaa attatttaag
    19561 atggctgctg atataatcta ctgcgccacc gactgtttca atttgttcag cgtcttcatc
    19621 tgaaatttcc gtaccgaatt catcttcaag ctctaaaacg aattccataa tactaattga
    19681 atcagctttt aaatcatctt tgatgttcaa ttcatccgtc actttatcgg tatctatctc
    19741 aaaatgattt gaaataattt tcgctacttt gttaaatact tcttcgcgag tcaacttttt
    19801 cacctccaag aatcatacac ttgtcaatta cctacaccat ggctaatttt actggcattt
    19861 ttcaggaatg tccacctttt tttcaatgga acggatcgct tcagatatcc aaatactatt
    19921 cttcgctttt ttcaaattgt tcgactaatt gtccaactac atctgtctca agcattgtgt
    19981 ggatttggcg gatcgtatag cgaacagcat cggggcctgt tgctccatga gttttaatga
    20041 ctggtgcttt caagccaaac aaaacggcac cgccatgttt ggaataatcc atttctgctt
    20101 tcaaagaacg taagccgtct tttaaaagca tagcgcccat ttttcctttg atcccttcat
    20161 ttagaatagc agattttaat agattcatca tattcattgc tgttccttca atagatttca
    20221 aaacagcgtt gcctgtaaat ccatctgtga ccactacatc agccacacca ttgagtagct
    20281 cacgagcttc tacgtttcca ataaaattca agcttgtttc attctgaagg agttcatagg
    20341 cttttttcgt cacttcacta ccttttgttg cttctgttcc gttgtttaag agtgcgacac
    20401 gaggacgtcc gatacctcgg actttacgtg cataaaaaga acctaaaata ccgtattgca
    20461 acaaatgttc tggtttgttt tctgcgttag cgccaagatc cagcatatcg aaaccggcac
    20521 cttctttccc tactactggc aaggtagaca tcaagccagg gcgttcgata tttttgatac
    20581 gtcctacgat gaacaaccct gctgctagta gcgcgcctgt attccctgct gaaaatacag
    20641 catctgcttc accgtttttc actgcttgtg cagcaaggac cattgatgca gtcttttttc
    20701 gtcgaattgc ttttactggc tcatcatcac tattgatttt ttcgtctgta tggataatcg
    20761 tgatgttttt ttcatttgtt aagtatttct tgatttctgc ttcttttccg taaagtaaga
    20821 attctacttc tggaaaatct tgctgcgcta aagccacgcc ttctacaatt gcttgaggtg
    20881 cgtgatctcc gcccattgca tctacggcaa ttttcattgt ttccactcct ttttccgtca
    20941 tcagtataaa gtatagcaca gtctgctaat cgaaataacg attatcctga ttttctattt
    21001 ggaggctttg cgctaaaggc tgatactcag gaagcatctg ccaatccttt atttgccaaa
    21061 gggctgcagc ctcttctctg gctacctcca aaacatttcc atccgtcacc aagtcagcaa
    21121 tcgcaaattg aggcagacca gattgacgga agccaaatac ttcaccagga ccacgtaatt
    21181 ccagatcttt ttcgcttagt acaaaaccat tggtggtctc tgtcatgatt ttcatccgtt
    21241 ccacacctag ttcattttta ggattagcaa ctaagataca gtaggaagca tcacttccgc
    21301 gtccaacacg tccacgaagt tggtgcagct gtgccaatcc aaatcgatcc gcatccatga
    21361 tcagcatgac ggtcgcgttc ggcacgttta cccctacttc aattacagta gtcgaaacta
    21421 atatctgcat ctggttgtct ttgaaggctt ccatgattac ttctttttcc tgattcttca
    21481 ttttaccatg taacaagcca acttcatatt ttggtgcaaa tagttcacga agtttttcat
    21541 agatttccac tgcatttttt acatctaatg cttctgattc ttcaatcagc ggacagatga
    21601 catacatttg atgtcctctt gccagttctt ttttcgtcca atccagtaca ctgtccaatt
    21661 gaggtgttct tacccagcgt gtttcgatcg ggatacgtcc agctggaagt tcatcaatga
    21721 tagatacatc catctcacca tatgctgtaa tagctaatgt tctaggaatc ggagtcgctg
    21781 tcataaataa gacatctggt cgccagcctt tttcacgaag tactttccgc tggttcacgc
    21841 caaaacggtg ctgctcatcc gtaataacta gacctaaatt ctgaaattcg actccatctt
    21901 ggatcaatgc atgtgtgccg atcacgacgt taatttcccc ttgctctagt ttttcaagga
    21961 gctcacgtcg ttcttttgct ttcgttgacc ccgtaatcaa tgcgatggtt acttcattgg
    22021 gatcaaacag ctgttgcaag ctttccatat gttgttgtgc caagatttct gtaggcacca
    22081 tcaacgcacc ttggaaacct gctgtaacgg tagcgtataa cgcaatcgcg gcaaccactg
    22141 tttttccgct acctacatct ccctgcaaaa gtcgctgcat atgctgcgga ctgcgaagat
    22201 cccgacagat ttcattcgtt acacgttttt gtgcatttgt cagttcaaaa ggtagctttt
    22261 gagtaaattg tttcaatttt tggacatcat aaaggatctc caaaccattc gtttctgctt
    22321 tttctgcttt tttcaatcct tgaagacgca tttgaaataa aaagaactct tcaaaaacga
    22381 cacgtcgttt ggcttgatgg ttttcttcgt ggtcttttgg aaaatgcata cttcttacag
    22441 cagaagctcg gtcaagcagt cgatatttct ccacaaggtt gtttggcaga ttttcttcaa
    22501 tcaatgtgcc gtattcttga aacccttttt tgattagatc aactaatgtc gtttgacgaa
    22561 ttgatttgtt gacgtgatat atcggcgaaa agtcatccat tgtttgagaa cctaaaatct
    22621 tcatcccgtt caaggcttta cgctttgcat cccatttacc gtatacggcg atttcctccg
    22681 ataaaatgac tttttctttt agataaggtt gattaaaaaa agaaacgttg aacacatcat
    22741 gttcttgcat catacgaaat tgcaatcggc tttttttata accaaatcga ttcatcacag
    22801 gaggcgagac tacgattcct ttcaaagtaa ccttttcttg atcctggatc tcgtttagat
    22861 ttctttcctg aatatcttcg tagcgaaaag gataataagt cagtaagtct tcaatcgttt
    22921 ggatgcccaa gctggcaaga ctagctgctc ttttttctcc gactcctgcc agcatagtga
    22981 ttggatcatc taaagaacgc ataaattttt cctccctcct ttaacaaaaa aaggacttgg
    23041 tcagcgatca tacctgatca ctaaccgaag gccttttatg aatgaatact gtgttagaaa
    23101 gatattctga cacagaaatt attcagcaga aaatagataa ggatagacag gctgatctcc
    23161 ttggtggatc tcgatttcca aatcaggatg tttttctgtt agtttctcag caaattcatt
    23221 tgcttgttca actgttcctt catcacctgc caagatcgtt acgatctctg tgtcctcatt
    23281 gatcatttgt tctagtgtac ttaacgctgt tgctgtgata tctgggttag agatcaggat
    23341 ttttccatcg atcatgccca aaaagtctcc ttctttgatc tctacaccgt caattgaggt
    23401 atctctgatc gcgtgagtga cttgaccgct cacaacgcta gacaacattt ctgtcatcgt
    23461 ttctttgttt tcttctaaag aagcttgatc attgaatgaa agcatagctg tcaatccttg
    23521 ggaaatcgtt cttgaaggaa caactgctac tgggatatct gctacttctg ctgcttgatc
    23581 agctgccatg aagatgttct tattgtttgg caagacaatc actttatctg cgtttacttc
    23641 ttgaatcgct ttaacaatgt cttccgtact tggattcatt gtttgtccac cactgataac
    23701 ataattagcg cctaaacttt caaacagctc ttggacgcct ttaccagcag cgatcgctac
    23761 gataccatac gggatacgtg gtatttcttt tttcgcaaac tcgttcactt cttcatcgtg
    23821 ttccaaaatc gtctcatgct gcaagcgcat gttgtcgact ttgattttga ctaatgaacc
    23881 gaatttttgt ccataattca agacttctcc tgggttttct gtatgcacgt ggactttgat
    23941 gatctcgtca tcattgacca caagtaaaga atctcctata ccatctaaat agttacggaa
    24001 ttcttcgtaa tcgaatgtac tatcgactgt aggtccttct ccgattttta ccatgatttc
    24061 tgtacagtaa ccaaaatgga tatcttctgt cgccatttga ccttggacac tgcgatgatg
    24121 ttctgcattg accatatcat ccatttctgc tggagaaggt tcatatgttt catccgcttg
    24181 gaattctcca ttcaatgcac ttaaaaagcc ctcataaaca aacagcagtc cttgccctcc
    24241 actgtctacg acaccgactt cttttaggac tggaagcaag tctggagttt tagctaaagc
    24301 acgctttgct ccttttacaa cagctgtcat tacttcaacg acatcatcag tactttttgc
    24361 tttcttttct ccagctttcg ctgcttctct agctactgtc aaaatcgttc cttctactgg
    24421 tttcattact gctttatacg cagtttcaac tccatgagtg aaggctgcag caagatcaga
    24481 agcatttaat gagacaacat ctggaatttg cttagaaaat ccacgaaaca attgagacaa
    24541 aataacgccg gaatttccgc gcgcacccat cagcaatcct tttgaaagca ttgctgctaa
    24601 ttctcctact ttttctgagg ttgaatctac tactgcctta gctccactag tcatagatag
    24661 attcatattt gttcccgtat cgccatctgg cacagggaat acatttaatg aattgacgta
    24721 ttctgcattt acttgcagac gattcgcacc tgcctggacc atttcctgga actggcttgc
    24781 acttatttct gttacactca cctagataat cctccttcag atcgactgct gagttaccag
    24841 ccagtctcct gcctagtctg gcagtacacg aacaccttgt acaaagacat taactgaatt
    24901 ggcagttacg cctaataatg cctctagatt gtatttcact ttttcttgga cgttgcggga
    24961 aacttcagag attttcgtac catagctgat aatcgtataa acatctacag caataccatt
    25021 atcttcttgg cggacaacta ctcctcttga gtaattttct ttacgcaaaa tctcagtaat
    25081 attatcttta atttgatttt tgctggccat tcctacaaca ccgaacacat ctgtcgcggc
    25141 gccaccaaca actgttgcaa ttacatcatt ggtaatttca atggttccag ctgacgtttg
    25201 aatttttaca gccatgaatg agcctcctta acgggtaaaa aactcccatt tttcttctat
    25261 catattttat catagcgcaa cttgaaataa aaggcagtta cccaaagtac tcaaaaacct
    25321 tgcaaaattt caggtataac cttgtttaat ttacaattat tcttctcaat tgtcaattgt
    25381 atctatgaaa atgtacttgc aaattcccat tctatatgat atgttagtct ggtatgagct
    25441 gaattattct attgctccaa atcaaggcaa aggaggaaat taccatggca aaagtttgtt
    25501 actttacagg tcgcaagaca aaaagtggaa acaaccgttc acacgctatg aactcaacaa
    25561 aacgtactgt taaacctaac ttacaaaaag ttcgcgttat ggtcgacgga aaacctaaaa
    25621 aagtttgggt gtcaactcgt gctttgaaat caggaaaagt tgaacgcgtt taattgaatg
    25681 atacttggac cgttcagata gtgaacggtt ttttatttaa taaaaatttt taagggtggg
    25741 acttttgatt cctacccttt ttttgcgttt atttt
//
LOCUS       DADOZM010000027        25394 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.027, whole genome shotgun sequence.
ACCESSION   DADOZM010000027 DADOZM010000000
VERSION     DADOZM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.027"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            147..794
                     /locus_tag="J8I47_000955"
     CDS             147..794
                     /locus_tag="J8I47_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAZ6322411.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1187..3832
                     /locus_tag="J8I47_000956"
     CDS             1187..3832
                     /locus_tag="J8I47_000956"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAZ6322412.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4142..5455
                     /locus_tag="J8I47_000957"
     CDS             4142..5455
                     /locus_tag="J8I47_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAZ6322413.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5445..6098
                     /locus_tag="J8I47_000958"
     CDS             5445..6098
                     /locus_tag="J8I47_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6322414.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6153..6704
                     /gene="radC"
                     /locus_tag="J8I47_000959"
                     /pseudo
     CDS             6153..6704
                     /gene="radC"
                     /locus_tag="J8I47_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6835..7920
                     /locus_tag="J8I47_000960"
     CDS             6835..7920
                     /locus_tag="J8I47_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6322415.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7917..9809
                     /locus_tag="J8I47_000961"
     CDS             7917..9809
                     /locus_tag="J8I47_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6322416.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9816..10193
                     /locus_tag="J8I47_000962"
     CDS             9816..10193
                     /locus_tag="J8I47_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6322417.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10347..11129
                     /locus_tag="J8I47_000963"
     CDS             10347..11129
                     /locus_tag="J8I47_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAZ6322418.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11255..11986)
                     /gene="erm(A)"
                     /locus_tag="J8I47_000964"
     CDS             complement(11255..11986)
                     /gene="erm(A)"
                     /locus_tag="J8I47_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAZ6322419.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12044..12131)
                     /locus_tag="J8I47_000965"
                     /pseudo
     CDS             complement(12044..12131)
                     /locus_tag="J8I47_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            12495..13157
                     /locus_tag="J8I47_000966"
     CDS             12495..13157
                     /locus_tag="J8I47_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ6322420.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13392..13535
                     /locus_tag="J8I47_000967"
                     /pseudo
     CDS             13392..13535
                     /locus_tag="J8I47_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13685..13885
                     /locus_tag="J8I47_000968"
     CDS             13685..13885
                     /locus_tag="J8I47_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6322421.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14139..15818)
                     /locus_tag="J8I47_000969"
     CDS             complement(14139..15818)
                     /locus_tag="J8I47_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAZ6322422.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15820..16032)
                     /locus_tag="J8I47_000970"
     CDS             complement(15820..16032)
                     /locus_tag="J8I47_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAZ6322423.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16425..18155
                     /gene="efrA"
                     /locus_tag="J8I47_000971"
     CDS             16425..18155
                     /gene="efrA"
                     /locus_tag="J8I47_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAZ6322424.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18152..19912
                     /gene="efrB"
                     /locus_tag="J8I47_000972"
     CDS             18152..19912
                     /gene="efrB"
                     /locus_tag="J8I47_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAZ6322425.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20007..20204
                     /locus_tag="J8I47_000973"
     CDS             20007..20204
                     /locus_tag="J8I47_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322426.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20357..20842
                     /locus_tag="J8I47_000974"
     CDS             20357..20842
                     /locus_tag="J8I47_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAZ6322427.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20946..21539)
                     /gene="clpP"
                     /locus_tag="J8I47_000975"
     CDS             complement(20946..21539)
                     /gene="clpP"
                     /locus_tag="J8I47_000975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAZ6322428.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21719..22246
                     /locus_tag="J8I47_000976"
     CDS             21719..22246
                     /locus_tag="J8I47_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAZ6322429.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22337..23074
                     /locus_tag="J8I47_000977"
     CDS             22337..23074
                     /locus_tag="J8I47_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAZ6322430.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23078..23995
                     /gene="miaA"
                     /locus_tag="J8I47_000978"
     CDS             23078..23995
                     /gene="miaA"
                     /locus_tag="J8I47_000978"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAZ6322431.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23997..25226
                     /gene="hflX"
                     /locus_tag="J8I47_000979"
     CDS             23997..25226
                     /gene="hflX"
                     /locus_tag="J8I47_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAZ6322432.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(25260..>25394)
                     /locus_tag="J8I47_000980"
     CDS             complement(25260..>25394)
                     /locus_tag="J8I47_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6322433.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT         8447 a         4132 c         5338 g         7477 t
ORIGIN      
        1 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
       61 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
      121 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
      181 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
      241 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
      301 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
      361 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
      421 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
      481 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
      541 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
      601 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
      661 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
      721 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
      781 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
      841 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
      901 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
      961 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
     1021 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
     1081 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
     1141 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
     1201 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
     1261 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
     1321 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
     1381 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
     1441 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
     1501 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
     1561 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
     1621 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
     1681 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
     1741 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
     1801 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
     1861 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
     1921 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
     1981 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
     2041 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
     2101 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
     2161 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
     2221 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
     2281 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
     2341 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
     2401 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
     2461 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
     2521 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
     2581 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
     2641 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
     2701 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
     2761 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
     2821 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
     2881 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
     2941 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
     3001 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
     3061 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
     3121 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
     3181 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
     3241 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
     3301 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
     3361 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
     3421 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
     3481 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
     3541 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
     3601 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
     3661 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
     3721 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
     3781 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
     3841 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
     3901 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
     3961 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
     4021 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
     4081 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
     4141 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
     4201 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
     4261 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
     4321 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
     4381 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
     4441 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
     4501 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
     4561 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
     4621 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
     4681 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
     4741 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
     4801 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
     4861 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
     4921 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
     4981 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
     5041 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
     5101 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
     5161 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
     5221 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
     5281 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
     5341 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
     5401 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
     5461 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
     5521 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
     5581 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
     5641 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
     5701 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
     5761 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
     5821 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
     5881 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
     5941 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
     6001 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
     6061 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
     6121 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
     6181 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
     6241 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
     6301 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
     6361 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
     6421 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
     6481 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
     6541 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
     6601 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
     6661 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tttaaccata gtataataaa
     6721 taatagtagt taaagtagtt taagaggaga tattaaaggg atattttact ttaaataaaa
     6781 attaacaagg tttataattt gtagtagtta aagtatttaa agaggtggga acatgtgaag
     6841 gttcaaagga tagaagtgga gaataagccg tatccattgt atttattact agataaagaa
     6901 taccagctaa tagaaccagt aatgaaattt attaaatact tagataatac tggtaagtct
     6961 cctaatacca ttaaagcata ctgctatcat ttaaagttgc tgtacgagtt catggaacag
     7021 agaggtgtta ttcttaatga tattaacttt gagttgttag cagacttcgt aggttggttg
     7081 agatatcctt cagcatcaaa tgtaattgat cttcagtcaa aaaaagccat aagagaagaa
     7141 acgacagtga atacaatttt aaatgtagtt atgagttttc ttgattattt aagtagatta
     7201 ggagaattta aatcaattga tgtatttaaa caagcaaagg gaagaaattt caaaggattt
     7261 ttacatcatg ttaataaggg tagataccaa aagaatgtct taaagttaag ggttaaaaag
     7321 aaacagataa gaacattgag atcagaggaa gttaagcaaa ttattgatgc ttgtcatacg
     7381 aaaagagata aattaatttt aatgcttatg tatgagggtg gtttaagaat cggtgaagtg
     7441 ttatcgctta ggcttgaaga tattgtcact tgggacaatc aaatccattt aacacctaga
     7501 gatgttaatg ttaatgaagc ttatattaaa ttaaggaagg aaagaacaat acatgtgagt
     7561 aaagaactta tgtcacttta tacagattac ttgatatatg agtatagtga ggaattggag
     7621 catgattatg tttttatttc cttaaaagaa ggctattttg ggaaaccact aaagtaccaa
     7681 agtgttcttg atctagttag aagaatagtt aaaaggactg gaatagaatt tacatcacat
     7741 atgcttcgcc acactcacgc aacgcagcta attagggaag gatgggatgt tgcgttcgtt
     7801 caaaagagat taggtcacgc acatgttcag acaacgttaa atacctatgt tcatctttca
     7861 gatcaggata tgaaaaatga gtttaataaa tacctcgaga gaaaggagca taagaaatga
     7921 atgcttctag taaaaggaaa attattagtc agagtgagat tagcaaaaaa atagctgtaa
     7981 tgaatgaaga aatgcagggg ttttgggcta ataatagttg ggatataaga aaatgtccac
     8041 atccttctgc catagaatta agtaagaatc ctgctttaag gaatcgttgg gttcgttttg
     8101 aacgtgttaa aaatctgtgg ttaagaacag aattgaaata tttttatttt taccatttaa
     8161 acaatggaat atggaatgca aaaactgtct ggattagaaa aggaacagta attaataaaa
     8221 tgttagattt cttagattta aagtatccta gcattacttc aattactgaa gttcctattg
     8281 aaaaagcaat gacggagtat agaacttatt tgacaaaacg gggtgttaga attaccacta
     8341 ctaattataa gattactgct aatcaagaaa aaacacctgt aaaagctaat tcctactatg
     8401 ttactaatct aaaacaattt atggagtttt atgagaactt ttattttgat ggagaggagt
     8461 gggataaaga cgtttgggat agacgtaact tacctttgcc agatgataag gttaacccaa
     8521 cacaatatga atatacaatt aactttaaag ggtttcggaa tacatatttt aaacaacttg
     8581 taaaaagata ttgtaagttg agattgaaca tggatagctt ttcctatgta agtgatattg
     8641 cccaaagact taaagagttc tttaattttc tggacatgaa atttaaacaa gttcagagag
     8701 tacaccaatt aacgagagtg gaaattgaag catatttaag tgaactaaac atgatgggaa
     8761 taaaacctag tacaataact gggaggatct ctatattgga aggactattt agtacccttc
     8821 ttaggctaga atgggatgat gttccttcca aaatattaat ttattctgag gactatccga
     8881 aaataccaag agcaaaacca cgctttatag atgaattcgt cctagagcaa ttgaacagtc
     8941 atcttgataa attacccgaa tatatagcta cgatgactat gattgttcaa gaatgtggaa
     9001 tgaggataag tgaattgtgc accttgaaaa aaggctgtct attagaggac aaagatggag
     9061 atttcttttt aaagtattat caatggaaaa tgaaaaagga gcatatagtt ccaatatcta
     9121 aagaggtagc tttacttatt aaagttcggg aagataaagt ttcagaggaa tttccagata
     9181 gtgaatacct ctttccaaga aaagatggat cgccattaaa acaagaaaca tttagaggtg
     9241 agttaaataa attagcttat gagcaaaata tagtggataa atcaggtgag atttatagat
     9301 tccatgccca tgcctttcgc catacagtag gaacaagaat gattaacaac gggatgcccc
     9361 agcatattgt gcagaaattt ttggggcatg aaagcccaga aatgacaagc agatacgctc
     9421 atatctttga tgaaactcta aaaaatgaat ttactaaatt tcaggaaaaa ctggttacca
     9481 ataatggaga tgtgcttgat ctagatgaag ataatgaagt cgatgatgta gagcttcaat
     9541 ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg ttattgtaga ttgccagtag
     9601 tagcaggtgg ttgtccacat gcgaatgcat gcttagattg cactcacttc tgtaccagta
     9661 agcaattctt accacagcac gaagaacagt tagagcgtac agaagagtta ttagccatag
     9721 ctaaggataa acaatggcaa agacaagtag agactaatag ccgtgttaaa gagcgtttag
     9781 aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt agaaatacaa
     9841 cagaaattgt acgtttggcg aagcagaaat caaaaaagac aagggaaaaa gtagacaaag
     9901 cgatttctaa attttcgatt gaaggtaaag ttattaattt taattcaata gcaaaggaag
     9961 ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga atcgaatccc
    10021 ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg
    10081 aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa gaaaataaaa
    10141 aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa taattattaa
    10201 tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt attgaacagt
    10261 catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa actttatatg
    10321 aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa ataccaaatc
    10381 aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata gttggagtat
    10441 atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta gatgttctag
    10501 tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa agactaatga
    10561 ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt acggttataa
    10621 ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata tacggtgagt
    10681 ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat cctgatttgg
    10741 ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct gattcttcaa
    10801 gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat tctttgccag
    10861 aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta gctcgaatgt
    10921 ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa tgggctatac
    10981 ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc tatcggggag
    11041 agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt aagtatatga
    11101 aaaattctat agaaacttct ctcaattagg ctaattttat tgcaataaca ggtgcttact
    11161 tttaaaacta ctgatttatt gataaatatt gaacaatttt tgggaagaat aaagcgtcct
    11221 cttgtgaaat tagagaacgc tttattactt taatttagtg aaacaatttg taactattga
    11281 aaatagaaag aaattgttcc ttcgatagtt tattaatatt agtgacattt gcatgcttca
    11341 aagcctgtcg gaattggttt ttagtgaaaa gaacacgata ttcacggttt acccacttat
    11401 aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat caatggttga tgtcgttcaa
    11461 gaacaatcaa tacagagtct acacttggct taggatgaaa atatagtggt ggtacttttt
    11521 tgagcatttt tatatccatc tccaccatta atagtaaacc caaagctcgt tgcagatttt
    11581 gcaatctttt cgcaaatccc ttctcaacga taagatagct atatttagcc tgactttcaa
    11641 aggtaattct tttgacaata tccgtactga tgttataagg aatattacca tatatcttat
    11701 agtttatatg ttttgggaag gaaaatttta gaatatccgt ttgaatcact tttatattct
    11761 cagaggggtt taccgcttct ttagtcactt gacataagcc tccatcaatt tctatagcag
    11821 taactgatcg actcattttg actagctctt tggtaaaatg tccttttcct gatccgattt
    11881 ctattacgtt gtcttgttta ctgatattcg tgtgattcaa tatttctttt acatgctttt
    11941 tagaagtaat aaaattttgc gtgtctttag ggtttttctg gttcattata accttctcct
    12001 tactggttat aatgaactga cattaagcgt tcattataac caattaattt tgatttggtt
    12061 gataacgaac tttattaata acaaatatag aaaaagtacc catactttta tctcctttcc
    12121 atttttttca ttatgaatga gataaagtaa tttctactac tgcgatactg gtgcacataa
    12181 ttgaaacctc cttatgtaac tgaatatgat tgtatcatct tttcatttta gaataaacct
    12241 ttatatgtaa ttttgcactt gacacttcag atggacgttt tcttcaaaat aatatgtttt
    12301 gaaacttgat aagagaagcc actgtcttta ctaaaaagta aaggctgcga taaaggacag
    12361 tgactttaat aaagttgcac tgttgtattg tctttggata aaattgtcta aagggaagtt
    12421 ttatccccaa cgaaaaaaca tctaaagtgg taagtaatga aagaatgtcg ataaagtagg
    12481 aatgggggat ctttttgaat acaatagaat cacagtttga taaggttgca gaagattacg
    12541 atttcgtgaa tgagcttttg aatgattatt cattctttgt gtctaatatg tctccaaaga
    12601 aaggcagagc attagatatc ggatgtggct cgggtttgtt agtggagaaa ttagcaagtt
    12661 attatgatga agtggtaggg attgatattt ctaatcaaat gctcgatctt gccaaatcta
    12721 aacgtcaact aacaaatacg gtctatctga atatgaatgc agaacaactt aattttaatg
    12781 agaagtttga ttttattgta agccgaacaa cctttcatca tttggatgat atagccagcg
    12841 taatacagca aatgaaggaa ctgttgaatg aggaaggaag aatagttatt cttgataatg
    12901 tatctgaagt cgagacaccg cctacctatg tttataaatt aggggcaatc caagagtttt
    12961 taccgcattg tttcaaattt ggaataaaaa atgcgattag aatttataat cacaacacat
    13021 ctaaatcatg gcttgagcat ttagcttcag ataagtacct atctgaacaa aattattacg
    13081 atttatatga aaagttgttg cctggatgcc aattccataa aatgggttgg gcaatggggg
    13141 ttgtctggac aaaataactt aaagctgtgg ctttgaaaag ttgaatttgt gattgttttc
    13201 agagctatcg ggtgctttag tataaataac aatgaataaa aaatcagcct gtatcttatg
    13261 ttactattaa gtaatttaag atacaggttt tactattttt gaaataagta ccttccactt
    13321 caaatacatg tattcaaagt gaaagtattg atttaaatga ttcttttgta atttactctt
    13381 gataatatga aattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg
    13441 gagaaatgat ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta
    13501 gtttgagaga agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa
    13561 gcaagtgtac aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac
    13621 ctctatcgta cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat
    13681 tattatggaa aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc
    13741 acgtgaagat ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa
    13801 aacattagaa gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca
    13861 agctgcaaac gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa
    13921 tttttacgag agaacaaagc gagtaacaaa aatacctgcc acaagagaca atttctcttg
    13981 tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa gaggctatga aagagccacc
    14041 ttgtatcaag gaataagaac aaacaaaaat ggcttctcat gtttttcatt tgttttctct
    14101 tatattcagg acaaggtgct gatttcataa cctcttattt attcttcatc tggtgtcatt
    14161 accattggca aaatcattgg atgtctttct gtacgttcga ataagaaagg ctgaagcgcg
    14221 ccagtcatcg cttctttcaa tttatattct gaacagtcag gtccattcaa acattctctt
    14281 aaagcgtgga ataagattcg ctgagcttca ttgatcattt ctcctgattc tcgcatgtag
    14341 acaaatccac gagagagaat atctggacca gccaagattt cttttttctt gatatccact
    14401 gtcgcgacag ctaatacaag accttcttct gaaagcaggt gacggtcacg taaaacgaca
    14461 ttaccaatat caccgatccc gctaccatcg atatacacat ctccagccac aaaatgccca
    14521 gcaggacgcg cagaatcgct agttaacgct aaaacatcgc cattttccat gatgaagcag
    14581 ttttctgcag gaacacctac atcttgtgct agagaagcat gtactttcaa catgcggaat
    14641 tctccgtgga caggaataaa atattttggc ttcatcaaac gaagcattaa tttttgttct
    14701 tcttgtccac cgtgaccgga agtatggatg ttattgattt ttccgtgaat cacttctgca
    14761 ccagcttcag acagcaaatt gatcaaatga ttgacacttg ttgtattacc aggaataggt
    14821 gaacttgaaa agatcactgt atcgtctggc tggatttgga tttgacggtg agtaccattc
    14881 gcgatgcggc tcaacgcggc catcgcttct ccttgagaac ctgtacatag gatcatcgtt
    14941 tcattggcag gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc tggaactttg
    15001 atatatccta aacgttgacc gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg
    15061 attttacgac cagttgccac agcggcatct gctgcttgct gcaaacggaa gatgttagaa
    15121 gcaaagctgg caaaaatgat acgtccttcg atttttgaaa agatcttcaa aatagaatgc
    15181 ccaatcgttt tttctgattt agtaaatgtt ggaatctcag catttgtact atcagataaa
    15241 aggcacaaga ctccttcttc cccgatacga gccattttat gcaagtttgc tggttcgcca
    15301 acaggagtga aatcgaattt gaagtctcca gtcgctacga tatttccaga aggtgttttc
    15361 accacgatcc ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct gacagaagtc
    15421 tttctaaaac ggatgatcgt atcttcattg atctcatgga gctctgcatc tcgaagcaag
    15481 ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc atagatagga
    15541 atatttgctt gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta
    15601 atcaccaagg cttttacttt gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca
    15661 tagtcaattc caagtaaatc gtcttccggg aatttgatcc cggcatcaat caaaatgatt
    15721 tcatcttgga attgtacccc atacgtattt ttcccgattt cacctaatcc accaatagca
    15781 aagacgcctg tctcgttatt tttgatggaa agtttcatat taaaactcca ctaatgagaa
    15841 atcagcattt tctttttcgt actctagatg acttccttct agaagttgaa cgaattcaat
    15901 attgtatggg gtattttctt ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat
    15961 gtaaagagat tttgtttctt cccgtttagg gttgcgtact tttgtttctt gataataaac
    16021 tttatagatc attgttttgt ctcctttatc tgcgtaaagc agtgatttag ctagttgatt
    16081 tttttagaca acacaaaacg aggggacaag ccacctggtt tttcatatgc tttgactaga
    16141 atactggatt cgagtcaaag agacaagcct cgtttttgct tatctgttcg gatatttttt
    16201 caaccgtacc ttctgtataa aaaatataca acaattaatt gttattctag cataaaacag
    16261 caaataagta tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc atcccgctat
    16321 aatcataatg acttttttat gagagaagtg ttctattaat aggctcgttg tgctatcatt
    16381 tttagtaatg aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc
    16441 attataccat gcgttataag aaattcttgt ttttcaattt catatgcgta tttggtttta
    16501 ttttgattga attaggactt cctacgattt tagcacgtat gatcgatgta ggaatccgca
    16561 atgatgattt tgattatgtg aagcaacaag ggatatttat gatcgttatt actgtaatcg
    16621 gtattatttt gaatatcttg ttaggttatt ttggttcgag gattacgact aatatcgtag
    16681 cagatattcg agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa
    16741 atctaggcgt atcttcattg atcactcgta caacaaacga tgcttatcag atcatgttgt
    16801 tcctgcaaaa tattttacgt atcggtttta tggcaccaat gatgttcatt gtcagtttgt
    16861 acatggtcat gcgtacaagt gtgacactca gtttgtatgt agtaggtgca ttacctttac
    16921 tattattggc agtcgtagcg attgctaagg tgtctgaacc gttatctaaa aagcaacaaa
    16981 aaaacttaga taaaatcaac agtatcttgc gggaaaatct ttcaggattg cgtgtgattc
    17041 gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat gagaattatg
    17101 cttctagttc aaaaagctta ttccgtttga tggctgttgc acaaccgggt ttttatttct
    17161 tatttaatat cgtaatggta ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg
    17221 agcttcgtgt aggagatttg attgcgttta tcgagtatat attccatgcc ttgttctcat
    17281 tcatgttatt tgctagtgtg ttcatgatgt atccaagagc agctgtttca gctggacgta
    17341 tccaagaagc cctagatgcc aaaccttcga ttgtagaaga tccaaatggt atatctgata
    17401 caaaaactaa agggtatatc gaatttcaaa atgtaacatt tgcttatcca ggccattcgc
    17461 aagagccagt gatacgaaat gtcagtttta cagcctctcc aggagagacc gttgctttta
    17521 ttggaagtac cggtagcgga aaatctacgt tgatccaatt gatccctcgc ttttatgatg
    17581 tgagtgaagg aaagatcttg atcgatggag tagacgtgag agattatcgg ctaagcaagt
    17641 tgcgagataa gattgggttt attccgcaaa aagcgctgct atttactgga acgattgcag
    17701 ataatttgcg atacgggaaa gaagatgcta cgcaagaaga aatggaacgt gcagctgaga
    17761 ttgctcaagc ttcagaattc atttctcgaa aaccagatgg atttaatgaa catttatcag
    17821 aaggtggttc caatttttca ggtggacaaa aacaacgatt ggcaatagcc agagccatca
    17881 tacgccgtcc ggaaatctat atttttgatg acagcttttc tgcattagat tatcaaacgg
    17941 atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga atctactgta ttgatcgtag
    18001 cacaacgtgt gggaacgatc atgcatgcag ataagatcat cgtattaaat gaaggagaag
    18061 tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg cccgatttat tatgatattg
    18121 ccgcttctca attatcaaag gaggaattag catgaaaaaa accttttctt ccttaaaacg
    18181 tttagctcga tatatcagac cgtatcgtga gacttttctt ttagtgatcc tatttacagt
    18241 tctgacagtg gctttcaacg tggctttgcc atatgttaca ggacttccta ctacagaaat
    18301 cagccgtaat attgccaatg gcgaatcaat caatttttct tatatattca aatgtctcat
    18361 ttggatcaca gttgttggaa tagggtattg tatttcgcaa ttactttctg gtgttttgat
    18421 gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga gacatagaag aaaaaatcaa
    18481 tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac
    18541 gaatgatgta gatgcagtca gcggtgcttt acaacaagct tttattggta tcgtaaatgc
    18601 gatattggga attacgttgg ctgcagcaat gatgttttat atccagccga tgatggcgct
    18661 gatctctatg atcatgattc caacatcgat ctggatctca aaacgagtga tcaatgcttc
    18721 gcagaaatat tttcaaagca tgcagaattc tcttggtgag ctaaacggct acgtccaaga
    18781 aaatatgaca ggttttagtg tattgaaagt atatggacgc gaaaaagaaa cattggaagg
    18841 attcaaaaaa gttaatcaca gcttgaaata ctttgggttt cgtgcagcat tcatttctgg
    18901 cttgatgatg cctctagttc aattaactgc ctatgctact tatattggta tggctgttct
    18961 aggaagcttt tatgttatta ctggggcgat cgttgtcggt caactacaag cattcatcca
    19021 atatatttgg caaatcagtc agccaatggg gaacgtgaca caattgtctt cagttttgca
    19081 aagtgcatca gcggcaacga aacgtgtatt cgagatttta gatgagccag aagaaaaaga
    19141 aaacaaagta gatgcttcac tgccagaaac gattttagga gatgtgacat tcgatcatgt
    19201 cgattttgca tatgatccga agaaaccatt aatccaagat ttgaattttg aagtgaaagc
    19261 tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc aagacgacat tgatcaatct
    19321 attgatgcgt ttttatgacg tagacaaagg agcaataagg attgatggaa tcgacaccaa
    19381 atcaatgagt cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag atgcatggct
    19441 ttatcaagga acgattgcag ataatatccg cttcggaaag cttgatgcga cagattatga
    19501 agtagttgat gctgcgaaaa cagcaaacgt agatcatttt atccgaacga tgccagatgg
    19561 atatgatatg gccatcaatt ctgagggaga caatgtgtct ttaggacaaa aacagttgct
    19621 gacgattgcc cgagcagtgg tctccgatcc taaaatcttg attttagatg aagcaacaag
    19681 ttcagtagac acccgtttag aagcgttgat tcaaaaagca atggatcgcg tgatggaggg
    19741 acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag atctgattct
    19801 agtgatggat caaggtcaga tcattgaaaa aggaacgcat gagagtttat tggcacaagg
    19861 tggattttat gaaaaactgt ataacagcca atttgctgag gaagcagaat aagtcatata
    19921 agaataaaac agtttaaaac ttattttttg ttctctattc ttgataatgg taaagtaaag
    19981 ttatcaagaa aagtgaggac acggagatgg aacagttttt aaatgaaaaa attactgaag
    20041 ccaaaagaga agttgatcgt ttaacgcacg tctgtcagac gaagcctttt gattcagctg
    20101 atttttttga aaatgagttt caaatcgaac gcttggcagc acaaatagaa gtgtacgaat
    20161 taattttaaa aaaagtaata gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc
    20221 agttaccaga tactgtaaat agacatctgg cctgagcagg ttttacctgt tttttttctt
    20281 atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta caaaaaatga
    20341 aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga ttggtgatgt
    20401 gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg acagtaattg
    20461 gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg tcgatcatga
    20521 tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc tacatggctg
    20581 tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc atgcggttat
    20641 tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag ttattcctcc
    20701 taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa aagaagaaat
    20761 acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc atgcgcaagg
    20821 aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga tggtcagttt
    20881 gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc ttcctttgaa
    20941 acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc atatgctttt
    21001 gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac ttcaagcggt
    21061 tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt
    21121 gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg atggatcatg
    21181 atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag caagaagctt
    21241 cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac aaagttcatt
    21301 gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat ataaagatag
    21361 atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat cgaattagca
    21421 agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa acgtgagtag
    21481 atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat taaattcata
    21541 atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt tctaatacct
    21601 aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc
    21661 gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga acaacagatt
    21721 gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg tctatatcgg
    21781 catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg aattggaatt
    21841 gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg agatcttgca
    21901 aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg attgttatat
    21961 cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt tttatctgcc
    22021 aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga ttttatctcc
    22081 agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga ttttagagaa
    22141 ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga agactatgac
    22201 gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt
    22261 gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac aatagaactg
    22321 aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc
    22381 agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact
    22441 agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag aagtggaccg
    22501 gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca aaaaattgaa
    22561 tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat tgagagaagt
    22621 gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa ttaaaactga
    22681 tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa gtagagattg
    22741 gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa tcgcattact
    22801 ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga tccaactagc
    22861 agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt tcgaacatcc
    22921 agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg atgaagaaga
    22981 aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc cggagaaagc
    23041 tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt
    23101 cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac tatttggagg
    23161 agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg gtacagcaaa
    23221 aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga tcgaacctac
    23281 tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta ccgagattgc
    23341 gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc aatcccttct
    23401 atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa aaaagtatga
    23461 aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag aaaaagaccc
    23521 ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag ctctggaagt
    23581 tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc aattgtatga
    23641 ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa tcaatcaaag
    23701 agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg
    23761 agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt ttgaagggaa
    23821 agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat acgcgaaacg
    23881 tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta taagtgagcc
    23941 aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg
    24001 aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt
    24061 caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta ttcacaatta
    24121 cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa ttacaagaat
    24181 tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat gagctaactc
    24241 cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc
    24301 ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg caggttgaac
    24361 ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc
    24421 ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca gaccgccgac
    24481 atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa gctcatcgag
    24541 agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt attggctaca
    24601 cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact tattcgaaag
    24661 atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct gaaggatttg
    24721 agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa ctgatcgatg
    24781 ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat gtagtcgatg
    24841 ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg gatgaactga
    24901 atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc gacccagcga
    24961 tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga
    25021 tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct tatacacttt
    25081 ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca atggttttgg
    25141 acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca aaaaccaatt
    25201 caaaatggtt aagaaaaaac caataaaatt ttattttttt aacaatatta tttgtcaaat
    25261 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
    25321 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
    25381 ttgacttggt tgaa
//
LOCUS       DADOZM010000028        24816 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.028, whole genome shotgun sequence.
ACCESSION   DADOZM010000028 DADOZM010000000
VERSION     DADOZM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.028"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(81..851)
                     /locus_tag="J8I47_000981"
     CDS             complement(81..851)
                     /locus_tag="J8I47_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322434.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(844..1779)
                     /locus_tag="J8I47_000982"
     CDS             complement(844..1779)
                     /locus_tag="J8I47_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322435.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2095..3744)
                     /locus_tag="J8I47_000983"
     CDS             complement(2095..3744)
                     /locus_tag="J8I47_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAZ6322436.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3861..6464)
                     /locus_tag="J8I47_000984"
     CDS             complement(3861..6464)
                     /locus_tag="J8I47_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ6322437.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6476..7525)
                     /locus_tag="J8I47_000985"
     CDS             complement(6476..7525)
                     /locus_tag="J8I47_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAZ6322438.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7551..7865)
                     /locus_tag="J8I47_000986"
     CDS             complement(7551..7865)
                     /locus_tag="J8I47_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAZ6322439.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7881..8327)
                     /locus_tag="J8I47_000987"
     CDS             complement(7881..8327)
                     /locus_tag="J8I47_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ6322440.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8328..10304)
                     /locus_tag="J8I47_000988"
     CDS             complement(8328..10304)
                     /locus_tag="J8I47_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAZ6322441.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10462..11358)
                     /locus_tag="J8I47_000989"
     CDS             complement(10462..11358)
                     /locus_tag="J8I47_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6322442.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11351..12031)
                     /locus_tag="J8I47_000990"
     CDS             complement(11351..12031)
                     /locus_tag="J8I47_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322443.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12032..12814)
                     /locus_tag="J8I47_000991"
     CDS             complement(12032..12814)
                     /locus_tag="J8I47_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ6322444.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12807..13724)
                     /locus_tag="J8I47_000992"
     CDS             complement(12807..13724)
                     /locus_tag="J8I47_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322445.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13888..14211)
                     /locus_tag="J8I47_000993"
     CDS             complement(13888..14211)
                     /locus_tag="J8I47_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322446.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            14999..18226
                     /gene="fss3"
                     /locus_tag="J8I47_000994"
     CDS             14999..18226
                     /gene="fss3"
                     /locus_tag="J8I47_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAZ6322447.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18339..18653
                     /locus_tag="J8I47_000995"
     CDS             18339..18653
                     /locus_tag="J8I47_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6322448.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18666..19040
                     /locus_tag="J8I47_000996"
     CDS             18666..19040
                     /locus_tag="J8I47_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6322449.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19050..19385
                     /locus_tag="J8I47_000997"
     CDS             19050..19385
                     /locus_tag="J8I47_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAZ6322450.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19468..20808
                     /locus_tag="J8I47_000998"
     CDS             19468..20808
                     /locus_tag="J8I47_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ6322451.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20886..21560
                     /locus_tag="J8I47_000999"
     CDS             20886..21560
                     /locus_tag="J8I47_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6322452.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            21793..22227
                     /gene="dcm"
                     /locus_tag="J8I47_001000"
     CDS             21793..22227
                     /gene="dcm"
                     /locus_tag="J8I47_001000"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAZ6322453.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            22228..22935
                     /locus_tag="J8I47_001001"
     CDS             22228..22935
                     /locus_tag="J8I47_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAZ6322454.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            22925..23215
                     /locus_tag="J8I47_001002"
     CDS             22925..23215
                     /locus_tag="J8I47_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322455.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            23474..24658
                     /locus_tag="J8I47_001003"
     CDS             23474..24658
                     /locus_tag="J8I47_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ6322456.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            24673..24792
                     /locus_tag="J8I47_001004"
     CDS             24673..24792
                     /locus_tag="J8I47_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAZ6322457.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         7723 a         4775 c         4632 g         7686 t
ORIGIN      
        1 aaacgacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg
       61 ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa
      121 gtaacggcgt acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc
      181 acatcgattg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg
      241 actttcgaaa cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct
      301 agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga
      361 gcaaaaatcc cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta
      421 atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt
      481 acgagagagc cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc
      541 acatgcgtag gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag
      601 aagaaggacg aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca
      661 ggcacgatga attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt
      721 tttccgaaaa taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc
      781 cagatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa
      841 agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc
      901 ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg
      961 atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc
     1021 ttcaaatgta caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa
     1081 gataaatgtt tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag
     1141 ttcacctgat tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg
     1201 cgtcgtcaaa atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc
     1261 gcgtctcaat tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc
     1321 atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc
     1381 acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat
     1441 cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt
     1501 ttcaaaggga ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac
     1561 tagatccgta tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa
     1621 agaagtaatg attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata
     1681 gaaatctcct tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata
     1741 aacctttttc aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag
     1801 acatgattct ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga
     1861 aaaatcaggt tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc
     1921 agacaacctt ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca
     1981 aaaggagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
     2041 ggcggaagca aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc
     2101 caatacatgc cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac
     2161 taaagtctct gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg
     2221 tccttctttt aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa
     2281 gtggacattc tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac
     2341 aaatgctaaa gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg
     2401 ttcgatcgta tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga
     2461 agccacttgg tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc
     2521 ttcaaataaa tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa
     2581 ctgttctgca atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt
     2641 gacatacgta gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt
     2701 gaccggattc actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc
     2761 gccctcagta aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc
     2821 agcggctact tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt
     2881 ttctaattta gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac
     2941 atattcaaaa atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt
     3001 agggatgtct cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag
     3061 ttttgctagc tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc
     3121 tgtttctagt gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat
     3181 tgccaaatag attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag
     3241 caagccaaga aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag
     3301 atattgtttt gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt
     3361 ttctctcagt tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc
     3421 accaaaatca aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt
     3481 actgttgatt ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat
     3541 ttcgctatcc catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc
     3601 tttatcaaat ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa
     3661 tttcaaacgt gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa
     3721 aacgataaat tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat
     3781 agcactgttg cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt
     3841 ttttcttgta tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga
     3901 ggaattgttg ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg
     3961 atagcctcag gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct
     4021 tgttcatgaa aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc
     4081 aatgaaaatt tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac
     4141 ctgtgaataa atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt
     4201 tctgcctgtt ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa
     4261 gtgactttat actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca
     4321 tttgtcgtat ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc
     4381 cctgttgttg aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa
     4441 ccatctgcat aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat
     4501 atttctaaat taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca
     4561 ggggtatttt caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa
     4621 acattaattg ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct
     4681 tcaacgattt gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg
     4741 ggtttttcag ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact
     4801 aatgtttctt gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct
     4861 gtatctcctt ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa
     4921 tgaacgaaat caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa
     4981 gaaagtttat atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat
     5041 ttttcattat ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt
     5101 atttttatgt caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc
     5161 atccgataag catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga
     5221 ctacctttga agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta
     5281 taattctcag tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca
     5341 gccatccgtt ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt
     5401 cgatggaaaa tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc
     5461 tggttttgca aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca
     5521 tttctttctg caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta
     5581 aaattttcta ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa
     5641 accggctgac gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct
     5701 acataagaag gataatctga aataatattg tggaatttac tgtatttatt caacttctct
     5761 acgacttgat caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata
     5821 tctttgtttt ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca
     5881 aattcaggaa tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga
     5941 aaattcatgg cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga
     6001 accatcgttt caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc
     6061 aaaagtgtag gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt
     6121 ggatctccat atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat
     6181 tcagcatcca ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct
     6241 gttttcatca gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg
     6301 acgattgaag tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct
     6361 agaacttcag taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat
     6421 tctctgtccc agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact
     6481 ttccttcttc tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat
     6541 tgaccattaa cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat
     6601 tcgtaacaaa aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa
     6661 atccatttag accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag
     6721 gatctttcac ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag
     6781 ctgtctttgc attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta
     6841 aaaacattcc aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc
     6901 ttccgcttga aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca
     6961 tatcaaacgc catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca
     7021 tgccttctag ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat
     7081 accataatat gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta
     7141 gactaataac ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag
     7201 ctaatccagc aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag
     7261 tgataaaagc tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa
     7321 tcatcggtac gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg
     7381 tatccgtctg gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc
     7441 ctgctgctac gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt
     7501 ctcttccttg tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta
     7561 attaatgcaa ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct
     7621 actgttaaaa tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca
     7681 aatatgacaa catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata
     7741 gccccctgtg tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta
     7801 gcttttgcca tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt
     7861 gtcatatgaa attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc
     7921 gaatctttta ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa
     7981 atacgtaaat gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca
     8041 actggttgat catctaatgc gccccattca acagaccgct ttaacttggc tacagccact
     8101 gttgattcaa ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct
     8161 gtggtcatat ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca
     8221 aacccattag tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca
     8281 tttaaatcta tcactatatt atttatattg attttttcac tgatcattta taagccttct
     8341 ttcaatatat aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca
     8401 tgattcgaag cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt
     8461 tcattaagtg caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct
     8521 ggattaacgt agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata
     8581 gcaaaatcat tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc
     8641 ccttctttcg cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact
     8701 tgattatcct ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa
     8761 gcatcttctg tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct
     8821 aacatcaaag gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt
     8881 ataacaagtt catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct
     8941 atctcaacca tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca
     9001 tatccgctac tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt
     9061 gattgaatat ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc
     9121 tcttcgattt cctctaataa atctacactt atcataaatg accttttaaa agaatatttg
     9181 attttgtccg tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca
     9241 ttgagatgta atactagatt attgatcaat tcttcatcag gataaaattc atttaattta
     9301 attcttacta actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct
     9361 aagtcgtgat tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt
     9421 tcatctattg gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta
     9481 ttctttgaga tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc
     9541 ctttctatcg caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc
     9601 aattgtgtct tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta
     9661 tttaagaaat tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta
     9721 ctgttagatg ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt
     9781 ttactttttt catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca
     9841 tgcttgttta gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg
     9901 tataattcgt cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca
     9961 aacacataag ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat
    10021 aaatttcgaa gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac
    10081 atttgtacat ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt
    10141 gttttcgtag atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca
    10201 atcaaaaagt atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc
    10261 tcctgaatac agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt
    10321 gattaatgta gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcctgcaat
    10381 atttatatca caggcttttt tctctattaa atatatataa gataatacat ttttctacta
    10441 gttctcacta atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc
    10501 cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta
    10561 aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg
    10621 gaagactttt tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg
    10681 aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa
    10741 attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt
    10801 atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa
    10861 agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt
    10921 ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc
    10981 aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc
    11041 cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc
    11101 gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt
    11161 ttcatccaac catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc
    11221 gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa
    11281 tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag
    11341 caacagccat ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat
    11401 gtgcctttgc tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat
    11461 caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg
    11521 gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac
    11581 cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt
    11641 gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca
    11701 aaagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg
    11761 aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct
    11821 gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga
    11881 tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa
    11941 gaagtttgta tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt
    12001 cgataatcaa aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa
    12061 acattcctag agctgatgat accacgattg ttactagact gaccagaatc attttcggtg
    12121 cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc
    12181 actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc
    12241 ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca
    12301 aagccactgg aacagataat gtccctaatg tcagcagaag caatacagga aatactttta
    12361 gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt
    12421 aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa
    12481 cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa
    12541 tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa
    12601 gaatccatgc agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat
    12661 ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat
    12721 aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata
    12781 cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag
    12841 aaataatctt ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct
    12901 accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg
    12961 ctagattgta caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc
    13021 gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat
    13081 aattctttga tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat
    13141 tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg
    13201 atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa
    13261 atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct
    13321 aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg
    13381 tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt
    13441 gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct
    13501 gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc
    13561 ataatagttc tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc
    13621 attccacgtt tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg
    13681 gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct
    13741 tattgatgag tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa
    13801 agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat
    13861 tatcaggcag tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt
    13921 cgtttctctg tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa
    13981 ttttaccaga ccggttataa ggataactat agcaagactc cctcccagaa aaattagact
    14041 gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc
    14101 aagtggactg gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg
    14161 cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta
    14221 aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa
    14281 gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc
    14341 aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc
    14401 ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg
    14461 tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag
    14521 aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt
    14581 gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc
    14641 gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg
    14701 ggctttctta agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt
    14761 tataaaggtt ttcccgtaaa tatattttat tatatttgat taaatttgaa atgtttgtgt
    14821 tctaaaatgt gttctaattt aaaggcagta tatttcgtat tttagattaa atttttaggc
    14881 tcattcattc tcaaatccat tctattcagt caataaaaat tttaaaaaga ctttataaac
    14941 aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag aacatttcat
    15001 gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaagc
    15061 aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc atccacaaac
    15121 ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg
    15181 tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct ttaaacaaga
    15241 tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg gctatgagaa
    15301 aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt
    15361 aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg
    15421 gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga
    15481 ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc ataatagtac
    15541 ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc
    15601 agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga atggtaatca
    15661 gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc
    15721 tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg
    15781 ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg agggttcttt
    15841 aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg ttttccattt
    15901 agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag gcattgctac
    15961 attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag taccggcgcc
    16021 ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa ccatttacgt
    16081 gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg gagttgaaac
    16141 agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg ccattcgcaa
    16201 agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga
    16261 aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga ccgaaactaa
    16321 agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga agtatgccaa
    16381 tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag tcactggcga
    16441 aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta aggcagtctt
    16501 tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta aatggagtga
    16561 ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg tcactttgac
    16621 tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt attattggca
    16681 agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg tatccatcaa
    16741 aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg caaaagaaca
    16801 agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg gcactaccga
    16861 aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca atgaaatcac
    16921 aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg
    16981 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg
    17041 ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca aagaagactt
    17101 tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac caattaaaac
    17161 ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt tgaaccgttt
    17221 aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga cttggaaaga
    17281 cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat tgagttacaa
    17341 tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggatgcgac
    17401 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc
    17461 aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat taaccgccga
    17521 acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata aggaagcttt
    17581 tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag cccaaactgt
    17641 cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag aaaacggttc
    17701 tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga ttactcatga
    17761 tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat taccggatgg
    17821 tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg ataaagaatt
    17881 caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag gaaccaagtt
    17941 tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac acaatgaaga
    18001 tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac taagtacgcc
    18061 gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc ccacagtgaa
    18121 gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg gttttacttt
    18181 gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg atgagatgat
    18241 acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt
    18301 tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc
    18361 cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa attataacag
    18421 aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat tccagtaagc
    18481 aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag gattttgatc
    18541 aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    18601 atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa
    18661 agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt gggaagttgc
    18721 gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca attggtaatg
    18781 aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg attcaagtta
    18841 gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagttggtca
    18901 atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc gttaattggt
    18961 tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc
    19021 aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat attttagatt
    19081 gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa gcattaacct
    19141 ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat agcgacagtg
    19201 aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt atagttgtcg
    19261 aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt ggtgtggaga
    19321 tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa ctcccctcct
    19381 cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc tattgggctt
    19441 tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg cgcaggagat
    19501 cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg
    19561 agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc actttctact
    19621 attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct cgctttttca
    19681 ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag cttgtggcat
    19741 cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa acaaactcaa
    19801 agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat cagttacttc
    19861 cccaaaatct gttatcgaat gaaagatgga ttactgtcta ttcgcgttca aatttcactt
    19921 gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg actgtattgt
    19981 gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact gtatgatatg
    20041 atcgccaatc gaattgggat agacgaagta gtggcagaaa atgggactct gcgattgatg
    20101 aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg tggcacgggt
    20161 ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc agatgcagaa
    20221 ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt gatgcctcat
    20281 gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga acgtatgatg
    20341 gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa ttatgcctat
    20401 cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat ggagatgtta
    20461 acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt
    20521 caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac
    20581 ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat
    20641 tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt
    20701 tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
    20761 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
    20821 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
    20881 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
    20941 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
    21001 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
    21061 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
    21121 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
    21181 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
    21241 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
    21301 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
    21361 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
    21421 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
    21481 attatgctac ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg
    21541 aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
    21601 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
    21661 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
    21721 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
    21781 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
    21841 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
    21901 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
    21961 tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg atttccgtgc
    22021 caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg tactttattc
    22081 tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct tgagaacgtc
    22141 cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat
    22201 ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat tccccaaagc
    22261 cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
    22321 atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct
    22381 tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca
    22441 ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt agcaggacta
    22501 ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc
    22561 ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa
    22621 gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag tcgccgaggt
    22681 cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc gatcgtcacg
    22741 gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc
    22801 gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta taagcaagcc
    22861 ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa agagttggat
    22921 gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa agattcagtc
    22981 agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca agaatggtgt
    23041 caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa
    23101 tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag atattcgtca
    23161 atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa
    23221 accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg
    23281 tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc cccgtatcta
    23341 acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc ttgttgtatc
    23401 aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac accttgaatt
    23461 tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag accgacgcaa
    23521 cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc ggccgtactt
    23581 aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga aaatttttga
    23641 ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact atgtaaggat
    23701 tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt tgaaatttga
    23761 gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg tcatgggtga
    23821 tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat taaaggggcg
    23881 aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt acgacttttt
    23941 cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg
    24001 agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc
    24061 cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg atgaaaagga
    24121 cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt tctgtttgta
    24181 tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa ctgagacgaa
    24241 aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc aagatttgtt
    24301 aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact tgcgatttgc
    24361 ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt
    24421 tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt ataccattga
    24481 acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt
    24541 ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac gcttatcaga
    24601 ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga ttatatgaaa
    24661 agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg cagtatgctt
    24721 ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc aatggaaaag
    24781 aaaggaaagt gacaagatga atttcggaca aaattt
//
LOCUS       DADOZM010000029        24372 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.029, whole genome shotgun sequence.
ACCESSION   DADOZM010000029 DADOZM010000000
VERSION     DADOZM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.029"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="J8I47_001005"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="J8I47_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAZ6322458.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /locus_tag="J8I47_001006"
     CDS             complement(1552..2973)
                     /locus_tag="J8I47_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6322459.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="J8I47_001007"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="J8I47_001007"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAZ6322460.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="J8I47_001008"
     CDS             complement(5384..6640)
                     /locus_tag="J8I47_001008"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAZ6322461.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="J8I47_001009"
     CDS             7220..8521
                     /locus_tag="J8I47_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAZ6322462.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="J8I47_001010"
     CDS             complement(8590..9189)
                     /locus_tag="J8I47_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6322463.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="J8I47_001011"
     CDS             9443..10324
                     /locus_tag="J8I47_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322464.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="J8I47_001012"
     CDS             10337..11947
                     /locus_tag="J8I47_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAZ6322465.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="J8I47_001013"
     CDS             complement(12024..12683)
                     /locus_tag="J8I47_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ6322466.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="J8I47_001014"
     CDS             complement(12966..13631)
                     /locus_tag="J8I47_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322467.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="J8I47_001015"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="J8I47_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAZ6322468.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="J8I47_001016"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="J8I47_001016"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAZ6322469.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="J8I47_001017"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="J8I47_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAZ6322470.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="J8I47_001018"
     CDS             15945..16265
                     /locus_tag="J8I47_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAZ6322471.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="J8I47_001019"
     CDS             complement(16377..17246)
                     /locus_tag="J8I47_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6322472.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18616)
                     /locus_tag="J8I47_001020"
     CDS             complement(17342..18616)
                     /locus_tag="J8I47_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ6322473.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="J8I47_001021"
     CDS             complement(18642..19151)
                     /locus_tag="J8I47_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322474.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="J8I47_001022"
     CDS             complement(19326..21461)
                     /locus_tag="J8I47_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6322475.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="J8I47_001023"
     CDS             complement(21544..23199)
                     /locus_tag="J8I47_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ6322476.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="J8I47_001024"
     CDS             complement(23201..23515)
                     /locus_tag="J8I47_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ6322477.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="J8I47_001025"
     CDS             23685..23945
                     /locus_tag="J8I47_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAZ6322478.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="J8I47_001026"
     CDS             23959..24273
                     /locus_tag="J8I47_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAZ6322479.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6945 a         5039 c         4393 g         7995 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
     9301 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
    10801 ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatccg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gacccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aa
//
LOCUS       DADOZM010000030        23830 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.030, whole genome shotgun sequence.
ACCESSION   DADOZM010000030 DADOZM010000000
VERSION     DADOZM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.030"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(55..789)
                     /locus_tag="J8I47_001027"
     CDS             complement(55..789)
                     /locus_tag="J8I47_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAZ6322480.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="J8I47_001028"
     CDS             1116..1526
                     /locus_tag="J8I47_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAZ6322481.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="J8I47_001029"
     CDS             1519..2220
                     /locus_tag="J8I47_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAZ6322482.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="J8I47_001030"
     CDS             2220..3806
                     /locus_tag="J8I47_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ6322483.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="J8I47_001031"
     CDS             3825..4046
                     /locus_tag="J8I47_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAZ6322484.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="J8I47_001032"
     CDS             4043..4804
                     /locus_tag="J8I47_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAZ6322485.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="J8I47_001033"
     CDS             5184..5693
                     /locus_tag="J8I47_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAZ6322486.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="J8I47_001034"
     CDS             complement(5768..6307)
                     /locus_tag="J8I47_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAZ6322487.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="J8I47_001035"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="J8I47_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAZ6322488.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="J8I47_001036"
     CDS             7374..8171
                     /locus_tag="J8I47_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAZ6322489.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="J8I47_001037"
     CDS             8199..8834
                     /locus_tag="J8I47_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAZ6322490.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="J8I47_001038"
     CDS             8854..9627
                     /locus_tag="J8I47_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6322491.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="J8I47_001039"
     CDS             10035..10832
                     /locus_tag="J8I47_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAZ6322492.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="J8I47_001040"
     CDS             10810..11223
                     /locus_tag="J8I47_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322493.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="J8I47_001041"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="J8I47_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAZ6322494.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="J8I47_001042"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="J8I47_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAZ6322495.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="J8I47_001043"
     CDS             16000..16560
                     /locus_tag="J8I47_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAZ6322496.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="J8I47_001044"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="J8I47_001044"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAZ6322497.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="J8I47_001045"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="J8I47_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAZ6322498.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="J8I47_001046"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="J8I47_001046"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAZ6322499.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="J8I47_001047"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="J8I47_001047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAZ6322500.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="J8I47_001048"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="J8I47_001048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAZ6322501.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="J8I47_001049"
     CDS             21907..22485
                     /locus_tag="J8I47_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAZ6322502.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="J8I47_001050"
     CDS             22537..23019
                     /locus_tag="J8I47_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAZ6322503.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="J8I47_001051"
     CDS             23136..23510
                     /locus_tag="J8I47_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322504.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6648 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DADOZM010000031        23516 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.031, whole genome shotgun sequence.
ACCESSION   DADOZM010000031 DADOZM010000000
VERSION     DADOZM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.031"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..258)
                     /locus_tag="J8I47_001052"
     CDS             complement(<1..258)
                     /locus_tag="J8I47_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322505.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="J8I47_001053"
     CDS             complement(274..924)
                     /locus_tag="J8I47_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322506.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="J8I47_001054"
     CDS             complement(935..2107)
                     /locus_tag="J8I47_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAZ6322507.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="J8I47_001055"
     CDS             complement(2126..2998)
                     /locus_tag="J8I47_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6322508.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="J8I47_001056"
     CDS             complement(3020..3940)
                     /locus_tag="J8I47_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322509.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="J8I47_001057"
     CDS             complement(3940..4209)
                     /locus_tag="J8I47_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322510.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="J8I47_001058"
     CDS             complement(4206..4586)
                     /locus_tag="J8I47_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAZ6322511.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="J8I47_001059"
     CDS             complement(5053..6516)
                     /locus_tag="J8I47_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ6322512.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="J8I47_001060"
     CDS             complement(6580..9597)
                     /locus_tag="J8I47_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6322513.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="J8I47_001061"
     CDS             complement(9748..10587)
                     /locus_tag="J8I47_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6322514.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="J8I47_001062"
     CDS             10732..10977
                     /locus_tag="J8I47_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322515.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="J8I47_001063"
     CDS             11040..11462
                     /locus_tag="J8I47_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322516.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="J8I47_001064"
     CDS             complement(11556..11873)
                     /locus_tag="J8I47_001064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322517.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="J8I47_001065"
     CDS             complement(11900..12106)
                     /locus_tag="J8I47_001065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322518.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="J8I47_001066"
     CDS             complement(12087..12269)
                     /locus_tag="J8I47_001066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322519.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="J8I47_001067"
     CDS             complement(12283..12633)
                     /locus_tag="J8I47_001067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322520.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="J8I47_001068"
     CDS             complement(12659..12961)
                     /locus_tag="J8I47_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322521.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="J8I47_001069"
     CDS             complement(12964..13197)
                     /locus_tag="J8I47_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322522.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="J8I47_001070"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="J8I47_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="J8I47_001071"
     CDS             complement(14628..15743)
                     /locus_tag="J8I47_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322523.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="J8I47_001072"
     CDS             complement(15749..16147)
                     /locus_tag="J8I47_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322524.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="J8I47_001073"
     CDS             complement(17176..17562)
                     /locus_tag="J8I47_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAZ6322525.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="J8I47_001074"
     CDS             complement(17559..18080)
                     /locus_tag="J8I47_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAZ6322526.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="J8I47_001075"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="J8I47_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAZ6322527.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="J8I47_001076"
     CDS             complement(19149..20564)
                     /locus_tag="J8I47_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAZ6322528.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="J8I47_001077"
     CDS             complement(20533..22686)
                     /locus_tag="J8I47_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAZ6322529.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="J8I47_001078"
     CDS             22944..23180
                     /locus_tag="J8I47_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ6322530.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="J8I47_001079"
     CDS             23199..23414
                     /locus_tag="J8I47_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ6322531.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         6629 a         4713 c         3609 g         8565 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttact
//
LOCUS       DADOZM010000032        23480 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.032, whole genome shotgun sequence.
ACCESSION   DADOZM010000032 DADOZM010000000
VERSION     DADOZM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.032"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(2022..2687)
                     /locus_tag="J8I47_001080"
     CDS             complement(2022..2687)
                     /locus_tag="J8I47_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6322532.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2813..4849
                     /locus_tag="J8I47_001081"
     CDS             2813..4849
                     /locus_tag="J8I47_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAZ6322533.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4853..5491
                     /locus_tag="J8I47_001082"
     CDS             4853..5491
                     /locus_tag="J8I47_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322534.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5519..5947
                     /locus_tag="J8I47_001083"
     CDS             5519..5947
                     /locus_tag="J8I47_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322535.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6404..7315
                     /locus_tag="J8I47_001084"
     CDS             6404..7315
                     /locus_tag="J8I47_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ6322536.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7532..7969
                     /locus_tag="J8I47_001085"
     CDS             7532..7969
                     /locus_tag="J8I47_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322537.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            8064..8948
                     /locus_tag="J8I47_001086"
     CDS             8064..8948
                     /locus_tag="J8I47_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAZ6322538.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            9172..12339
                     /locus_tag="J8I47_001087"
     CDS             9172..12339
                     /locus_tag="J8I47_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322539.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            12359..12904
                     /locus_tag="J8I47_001088"
     CDS             12359..12904
                     /locus_tag="J8I47_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAZ6322540.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12932..13387
                     /locus_tag="J8I47_001089"
     CDS             12932..13387
                     /locus_tag="J8I47_001089"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAZ6322541.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            13384..14142
                     /locus_tag="J8I47_001090"
     CDS             13384..14142
                     /locus_tag="J8I47_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ6322542.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(14228..14968)
                     /locus_tag="J8I47_001091"
     CDS             complement(14228..14968)
                     /locus_tag="J8I47_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322543.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            15029..16282
                     /locus_tag="J8I47_001092"
     CDS             15029..16282
                     /locus_tag="J8I47_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ6322544.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16604..16831
                     /locus_tag="J8I47_001093"
     CDS             16604..16831
                     /locus_tag="J8I47_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAZ6322545.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16954..17280
                     /locus_tag="J8I47_001094"
     CDS             16954..17280
                     /locus_tag="J8I47_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAZ6322546.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            17348..18061
                     /locus_tag="J8I47_001095"
     CDS             17348..18061
                     /locus_tag="J8I47_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAZ6322547.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            18371..19222
                     /locus_tag="J8I47_001096"
     CDS             18371..19222
                     /locus_tag="J8I47_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6322548.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            19343..21208
                     /locus_tag="J8I47_001097"
     CDS             19343..21208
                     /locus_tag="J8I47_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6322549.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            21256..22665
                     /locus_tag="J8I47_001098"
     CDS             21256..22665
                     /locus_tag="J8I47_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAZ6322550.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22773..23474
                     /locus_tag="J8I47_001099"
     CDS             22773..23474
                     /locus_tag="J8I47_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6322551.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         7913 a         3809 c         4809 g         6949 t
ORIGIN      
        1 ccatttaaat acccacaaga aatgaagcga gcatggcgta aaatgttaga tgatgagttg
       61 attccacttg agaatgaatt gaggtaaacg agaagagtta agaaaattgt acgattcaaa
      121 gatggtagtc aagtggaagt gtagaaaata gggatagatg tcttttattt gttcacattt
      181 tataacgaag tatttaagac agctattgaa taatgtttcc tccacctttt tcattcattt
      241 accccacccc gttgttatat gaccgtgttt tgagaggaac caataaaaag cataggctaa
      301 ctaggggttc ctttttcttt tgtttttttg gaatccgctt aagtgaaatg tatctgaaat
      361 gaaacggaga taaagtgatt ttttgctata atgacaaggt tttagctgtt tcagatacca
      421 tgtaacccaa aatagtttat tgagaaaata ggagagtatt tgatactaac tagtaggaaa
      481 aaatgagata aaaattaaat ggaatcattt caggtaatct caaataaaaa aatatacagt
      541 cctgtggcac tttacctatg ggactgtata ttttttactc tcagcgggct tgttttgact
      601 ccgtgtctga ctccgtcgta ttgattttaa atgatattcg atgaccattt aacaggtcat
      661 ttttgaaatt tttgtctcta tatgactcag tttgataatt attgatagat caatttgggt
      721 ctatatcgaa caagggaata atccaataaa aaatgattta gaatatcaaa ataacaaaaa
      781 agaggactac ctgtattcgt cctctttttt gaccagattt tgaccagtct aatttacatt
      841 ctaatatatt catatgagct aattttttga ttaaatggta agtttttcct tgtttgtttt
      901 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
      961 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
     1021 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
     1081 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
     1141 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
     1201 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
     1261 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
     1321 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
     1381 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
     1441 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
     1501 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
     1561 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
     1621 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
     1681 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
     1741 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
     1801 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     1861 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     1921 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1981 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     2041 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     2101 ggaatggctt cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt
     2161 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     2221 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     2281 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     2341 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     2401 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     2461 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     2521 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     2581 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     2641 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     2701 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     2761 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     2821 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     2881 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     2941 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     3001 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     3061 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     3121 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     3181 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     3241 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     3301 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     3361 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     3421 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     3481 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     3541 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     3601 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     3661 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     3721 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     3781 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     3841 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     3901 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3961 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     4021 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     4081 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     4141 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     4201 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     4261 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     4321 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     4381 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     4441 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     4501 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     4561 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     4621 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     4681 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     4741 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     4801 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     4861 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     4921 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4981 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     5041 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     5101 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     5161 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     5221 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     5281 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     5341 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     5401 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     5461 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     5521 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     5581 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     5641 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     5701 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     5761 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     5821 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     5881 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg
     5941 gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa cagcgttaaa
     6001 agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa
     6061 aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc
     6121 agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac
     6181 ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt
     6241 gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc
     6301 aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg
     6361 tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt
     6421 agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa
     6481 aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa
     6541 aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga
     6601 taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa
     6661 acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt
     6721 caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt
     6781 gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat
     6841 tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga
     6901 cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg
     6961 aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt
     7021 taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac
     7081 tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa
     7141 atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca
     7201 gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt
     7261 tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt
     7321 ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat ctttttatta
     7381 gaactaaatc aacagtagtt taattactac ttgatttagt tctttttatt ttaaaaattt
     7441 tttttactta gaatccagcg accctagata tttttctgta taatcttaaa taaagaaaac
     7501 aatatcaaaa aatgcacgta gaagtagata aatggagtgg ttttgcaaat ttcttttatt
     7561 tctagaagtt attcacgtat acggatttgt ttttctcaca aataaaaagg caggaagtac
     7621 aatggatcat ttgtatataa aatatattca tcaattttcc gttttggtgt taggcacact
     7681 aattatagcg gggattgtcg catttattat tcaaggccgt gacatattaa cagtacatca
     7741 agagtttttc agttatgcat ttggtattcc tctgatcgct ataaggtctg ataacaaaaa
     7801 gtggaagaaa cgctattaca ttctcttgct atttttgtgt ttttatctgc cacctaagat
     7861 ttttacttta ttaggtatac aatgcctatc attttctttt atttcctata tcattgtttt
     7921 aattttcctc agtttcatag tatggatgtt ttatcttgtc aaaaaataaa tgaatgtttc
     7981 atgaacagta cttcatcaca gcctaatttt tttctttatg ctatcgttat attaaagaaa
     8041 tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt agtggtaaac cccagttcag
     8101 gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc aaaattggaa tcggtgtttg
     8161 atgaagtggt cgtcttgcat acaaaaaaag caggggatgc aaaaaacttt actcgcgaag
     8221 cggctacgga agggtatcat agtgtgtttg tcatgggcgg agatggaaca gtcaacgaag
     8281 gaatcagtgg aatcgctgaa caagaacatc ggccaaattt cggatttttc ccattgggta
     8341 cggtgaatga cttggctcga gcattaggga taccgctaga acctgaggaa gcgatcaatc
     8401 atttttcgat tgaatcagtc aagccattgg atattggaaa aataaatgac gactatttta
     8461 tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa tgatgtagat gcggagaaga
     8521 agacgaaatt tggaaaacta gcttatttca tgtcaggaat caaacagtta gcttccacac
     8581 aatcctactc gtttcacgta gaagttgatg gaaagaagga agaaattgag agcagtacct
     8641 tgctgatagg actaactaac tcagtcggcg gttttgaaac attacttcca aatgcaaaag
     8701 tggatgatgg taagctccat ttggtttatt taaaagattc ttctttgatc gatacgctta
     8761 aaacattgcc agacctatta aaaggagtag atgaatctac gaaaaatcta gcctacctta
     8821 cttgtgagca attagttgtg tcattagctg atgaggaaga attgacgacg aatgttgatg
     8881 gagatgaagg ggataaatta cctgtgacaa tcaaagtatt gccctcacat ttacaggttt
     8941 attgttaaaa tggaggttgt gaccgaggag ttggcgggag aacaccgctt gttcgggata
     9001 agggactgtg acaagacttt tgtcacagcc ttttttttgt acaaaaaaga aatttacacc
     9061 taatagaaaa atttttccat aaaaatacat attttaaata tgaaagtaca attggaaata
     9121 gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa
     9181 tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc
     9241 gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca aagttttgac
     9301 aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca aagtgaggct
     9361 tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt agctgaacaa
     9421 acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg gtacagcgaa
     9481 gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac taagatccga
     9541 ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat cgttattgat
     9601 tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga gatacctcag
     9661 tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt gacaccaaat
     9721 atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag cagatatcat
     9781 tttacgggag aaataaaatt taccgggaca cttgatttgt atgatgggtc caaattagga
     9841 ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt gttggatgtc
     9901 caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg tagaagctat
     9961 caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa attctacggt
    10021 tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat ccatatctta
    10081 agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga tgaaggagag
    10141 gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg aagcgaattt
    10201 tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat ccaaatgaga
    10261 ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa tacagcaact
    10321 acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag tggtggacaa
    10381 atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat gcgttttgta
    10441 ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat cgataagcag
    10501 agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac agtaaccaat
    10561 ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa cagactcaat
    10621 caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag tccaggttca
    10681 gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga ttccggagca
    10741 actgtagata caactggaga tattgattta aattttacgg caggtgaaaa aacttattta
    10801 gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga tgtgttgaat
    10861 gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg aagcgagacc
    10921 aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa gatcgcccat
    10981 tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga aaaagacccg
    11041 gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg caatttcgta
    11101 tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg aatgaaaaat
    11161 tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag agtgccaacg
    11221 aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga ggcaaagcca
    11281 agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc agatacgacg
    11341 aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa tgaaggggca
    11401 ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca agtacccaat
    11461 gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa agtaagaggt
    11521 ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat tcctcctgaa
    11581 cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac aggtacaggt
    11641 gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga aacaactatt
    11701 gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc aggagatgta
    11761 ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt gcagaacaag
    11821 ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt tgtttatcat
    11881 gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct ctcatttctt
    11941 gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca agaagcattt
    12001 ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac gaaaaatccg
    12061 tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt ttctatgcag
    12121 gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga agaactggaa
    12181 attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata tttaaataac
    12241 ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa ttactcagca
    12301 gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag gatgattgat
    12361 gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga tccttctcct
    12421 tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg cggtagattt
    12481 tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa ggatggagcc
    12541 tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat cagtaacagt
    12601 tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat atggagaatc
    12661 aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa aaacagtggg
    12721 agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga aagcgccaga
    12781 aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa aagagaacgt
    12841 ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag caatcttgat
    12901 ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt atgcggatca
    12961 aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt cttatatgtt
    13021 cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt acctttcaat
    13081 aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg tcccattaga
    13141 aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt ggaaagaaaa
    13201 acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc aattgaatga
    13261 aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc tgatacttgt
    13321 tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa gaaggaaaaa
    13381 gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt attatgtaca
    13441 caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat ttctgtgacc
    13501 aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga tccaaatgat
    13561 cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt actgacgatt
    13621 gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa aacagtatat
    13681 gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca gaatttcgta
    13741 caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa acgaacagaa
    13801 tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa attcaaaaac
    13861 gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt ttctggaact
    13921 ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac tgcaggagca
    13981 aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca agggagacaa
    14041 tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt ttcaacattc
    14101 acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat caacgatttc
    14161 agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac gtctcagctc
    14221 ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt gtcttttcaa
    14281 agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca taatgagcag
    14341 aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt aaaaagtaag
    14401 catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat ttatcttctg
    14461 tagttgtttt gaccataact tggtttttca aacctttgat actttcaata tataaaattt
    14521 cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta tcgacattta
    14581 cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt tcagataaat
    14641 tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc tctactgcaa
    14701 aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta cgtatttgtt
    14761 cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag aaaacatcgt
    14821 gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa gtgagctgca
    14881 cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg tgaaactggt
    14941 tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa tatgtgtaca
    15001 ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt atttgacctt
    15061 tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc tagtaaaaaa
    15121 cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc ctaaagtgag
    15181 ctttccttac ctagttggct tattattgat gtttcttttt tattttacta cgaaaaacct
    15241 gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca gcttttttct
    15301 ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa gtttttttgc
    15361 actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac agacgagatt
    15421 tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt gtattttatt
    15481 aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat ctaattatct
    15541 gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt ccattatgtt
    15601 gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt atcaaataaa
    15661 tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc tactgttttc
    15721 attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag aaatattaga
    15781 agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa atattgtatc
    15841 cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg agaaaatccc
    15901 gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg ttgtttttat
    15961 tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg atcagagtcc
    16021 gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag cgaacaaggc
    16081 aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct ctaaaaaaaa
    16141 tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa atgcagaact
    16201 aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc tggcatctag
    16261 aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa agtctacacg
    16321 gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga ccgttgtcac
    16381 ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc tacttagtat
    16441 tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg gaacccacga
    16501 tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat atcgcttatt
    16561 ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga cccagcagtt
    16621 aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag tcccgcctaa
    16681 agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag tagcgttgtc
    16741 tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag acacgctact
    16801 gcatacacat ggtggctacc taaattattg attttctatg acatttattt taaataaaag
    16861 aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg gtatgattaa
    16921 tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa agtcaaccag
    16981 tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc tatctctatt
    17041 tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga agaagtaaaa
    17101 gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta ttcatacatg
    17161 atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt gatcaaaaat
    17221 aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag
    17281 aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga gtctggaggg
    17341 agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct
    17401 cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt attttgtcag
    17461 agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa
    17521 cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag aaggggtcat
    17581 gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag tcccttcagt
    17641 ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt acggagcagg
    17701 aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa gccatcgtgc
    17761 aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg ccaagatcta
    17821 tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa aagttgctcc
    17881 tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac taattacttg
    17941 tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa gtgttacatc
    18001 gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa aaacatttta
    18061 ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga atcgataagg
    18121 aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga tttggttgtt
    18181 ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct tgcataaata
    18241 aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac ggcaggcaaa
    18301 accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt tcttgagagg
    18361 gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg
    18421 attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa agattgggga
    18481 cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt ttgctaaaaa
    18541 ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg tcaaacaagt
    18601 agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt atctgacatt
    18661 gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac tgcctaatcc
    18721 attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga aagaggctct
    18781 tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg gttttattgc
    18841 ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga tgaccgctac
    18901 cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg tgttcgatga
    18961 agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc agcgttatct
    19021 caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc aaggaaagta
    19081 tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca
    19141 ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag tagtaagtga
    19201 gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc tggattgaaa
    19261 aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat tttttaaata
    19321 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
    19381 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
    19441 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
    19501 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
    19561 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
    19621 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
    19681 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
    19741 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
    19801 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
    19861 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
    19921 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
    19981 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
    20041 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
    20101 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
    20161 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
    20221 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
    20281 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
    20341 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
    20401 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
    20461 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
    20521 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
    20581 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
    20641 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
    20701 agacaatcaa ttagaaaaca aacatgcgaa tgcaggaaaa ccgattactt cagcacggca
    20761 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
    20821 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
    20881 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
    20941 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
    21001 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
    21061 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
    21121 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
    21181 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
    21241 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
    21301 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
    21361 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
    21421 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
    21481 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
    21541 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
    21601 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
    21661 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
    21721 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
    21781 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
    21841 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
    21901 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
    21961 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
    22021 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
    22081 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
    22141 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
    22201 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
    22261 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
    22321 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
    22381 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
    22441 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
    22501 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
    22561 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
    22621 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
    22681 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
    22741 cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta ctatacttgg
    22801 aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc ggttcgatgt
    22861 tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact gtccgaaagg
    22921 caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg
    22981 ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg caaatcttgg
    23041 agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc catataaaga
    23101 aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag cgcttatatt
    23161 atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa atgatgcaat
    23221 tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa aaaggatata
    23281 ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa gaacaaggaa
    23341 ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct acgcaaattg
    23401 gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct taccatattg
    23461 attatgaaac ataaataaaa
//
LOCUS       DADOZM010000033        22991 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.033, whole genome shotgun sequence.
ACCESSION   DADOZM010000033 DADOZM010000000
VERSION     DADOZM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.033"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(109..564)
                     /locus_tag="J8I47_001100"
     CDS             complement(109..564)
                     /locus_tag="J8I47_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322552.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="J8I47_001101"
     CDS             complement(718..1926)
                     /locus_tag="J8I47_001101"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAZ6322553.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="J8I47_001102"
     CDS             complement(2038..2370)
                     /locus_tag="J8I47_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAZ6322554.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="J8I47_001103"
     CDS             2518..3393
                     /locus_tag="J8I47_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6322555.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="J8I47_001104"
     CDS             complement(3475..4056)
                     /locus_tag="J8I47_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAZ6322556.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="J8I47_001105"
     CDS             complement(4062..5321)
                     /locus_tag="J8I47_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAZ6322557.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="J8I47_001106"
     CDS             complement(5323..6369)
                     /locus_tag="J8I47_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322558.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="J8I47_001107"
     CDS             complement(6604..6879)
                     /locus_tag="J8I47_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAZ6322559.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="J8I47_001108"
     CDS             complement(7122..8432)
                     /locus_tag="J8I47_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAZ6322560.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="J8I47_001109"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="J8I47_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAZ6322561.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="J8I47_001110"
     CDS             complement(9978..10658)
                     /locus_tag="J8I47_001110"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAZ6322562.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="J8I47_001111"
     CDS             complement(10707..11342)
                     /locus_tag="J8I47_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6322563.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="J8I47_001112"
     CDS             complement(11408..12811)
                     /locus_tag="J8I47_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAZ6322564.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="J8I47_001113"
     CDS             complement(12798..13829)
                     /locus_tag="J8I47_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322565.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="J8I47_001114"
     CDS             13860..14081
                     /locus_tag="J8I47_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAZ6322566.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="J8I47_001115"
     CDS             complement(14246..14983)
                     /locus_tag="J8I47_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAZ6322567.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="J8I47_001116"
     CDS             complement(15136..16476)
                     /locus_tag="J8I47_001116"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAZ6322568.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="J8I47_001117"
     CDS             complement(16713..18236)
                     /locus_tag="J8I47_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAZ6322569.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18613..19224)
                     /locus_tag="J8I47_001118"
     CDS             complement(18613..19224)
                     /locus_tag="J8I47_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ6322570.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19347..19472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19554..20270)
                     /locus_tag="J8I47_001119"
     CDS             complement(19554..20270)
                     /locus_tag="J8I47_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAZ6322571.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20276..20845)
                     /gene="scpB"
                     /locus_tag="J8I47_001120"
     CDS             complement(20276..20845)
                     /gene="scpB"
                     /locus_tag="J8I47_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAZ6322572.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20829..21626)
                     /locus_tag="J8I47_001121"
     CDS             complement(20829..21626)
                     /locus_tag="J8I47_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAZ6322573.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21596..21967)
                     /locus_tag="J8I47_001122"
     CDS             complement(21596..21967)
                     /locus_tag="J8I47_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAZ6322574.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21986..22873)
                     /gene="xerD"
                     /locus_tag="J8I47_001123"
     CDS             complement(21986..22873)
                     /gene="xerD"
                     /locus_tag="J8I47_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAZ6322575.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6445 a         4914 c         3898 g         7734 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aagaccaaga aatttcttga
    18601 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    18661 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    18721 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    18781 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    18841 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    18901 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    18961 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    19021 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    19081 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    19141 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    19201 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    19261 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    19321 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    19381 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    19441 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    19501 tatacatatc tattcaaaaa acgcaacatc taacaattat aaaaagtaag tgttcatttt
    19561 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    19621 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    19681 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    19741 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    19801 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    19861 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    19921 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    19981 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    20041 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    20101 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    20161 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    20221 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    20281 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    20341 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    20401 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    20461 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    20521 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    20581 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    20641 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    20701 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    20761 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    20821 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    20881 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    20941 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    21001 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    21061 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    21121 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    21181 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    21241 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    21301 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgcc gaattcttgg
    21361 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    21421 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    21481 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    21541 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    21601 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    21661 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    21721 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    21781 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    21841 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    21901 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    21961 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    22021 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    22081 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    22141 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    22201 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    22261 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    22321 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    22381 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    22441 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    22501 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    22561 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    22621 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    22681 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    22741 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    22801 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    22861 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    22921 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    22981 gtttcttttt t
//
LOCUS       DADOZM010000034        22031 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.034, whole genome shotgun sequence.
ACCESSION   DADOZM010000034 DADOZM010000000
VERSION     DADOZM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.034"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            367..555
                     /locus_tag="J8I47_001124"
     CDS             367..555
                     /locus_tag="J8I47_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322576.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="J8I47_001125"
     CDS             545..805
                     /locus_tag="J8I47_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322577.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="J8I47_001126"
     CDS             846..1073
                     /locus_tag="J8I47_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322578.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="J8I47_001127"
     CDS             1138..1710
                     /locus_tag="J8I47_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322579.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="J8I47_001128"
     CDS             1711..2022
                     /locus_tag="J8I47_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ6322580.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="J8I47_001129"
     CDS             2013..2273
                     /locus_tag="J8I47_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322581.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="J8I47_001130"
     CDS             2434..2793
                     /locus_tag="J8I47_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322582.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="J8I47_001131"
     CDS             2790..4433
                     /locus_tag="J8I47_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ6322583.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="J8I47_001132"
     CDS             4468..5550
                     /locus_tag="J8I47_001132"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322584.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="J8I47_001133"
     CDS             5594..6766
                     /locus_tag="J8I47_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ6322585.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="J8I47_001134"
     CDS             6753..7457
                     /locus_tag="J8I47_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAZ6322586.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="J8I47_001135"
     CDS             7462..8622
                     /locus_tag="J8I47_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ6322587.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="J8I47_001136"
     CDS             8698..8976
                     /locus_tag="J8I47_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAZ6322588.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="J8I47_001137"
     CDS             8957..9328
                     /locus_tag="J8I47_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAZ6322589.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="J8I47_001138"
     CDS             9344..9748
                     /locus_tag="J8I47_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322590.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="J8I47_001139"
     CDS             9745..10155
                     /locus_tag="J8I47_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322591.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="J8I47_001140"
     CDS             10216..10803
                     /locus_tag="J8I47_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ6322592.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="J8I47_001141"
     CDS             10876..11343
                     /locus_tag="J8I47_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAZ6322593.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="J8I47_001142"
     CDS             11389..11697
                     /locus_tag="J8I47_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322594.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="J8I47_001143"
     CDS             11892..18626
                     /locus_tag="J8I47_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAZ6322595.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="J8I47_001144"
     CDS             18630..19502
                     /locus_tag="J8I47_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322596.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="J8I47_001145"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="J8I47_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="J8I47_001146"
     CDS             20087..20548
                     /locus_tag="J8I47_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322597.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="J8I47_001147"
     CDS             20552..21376
                     /locus_tag="J8I47_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322598.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..21939
                     /locus_tag="J8I47_001148"
                     /pseudo
     CDS             21394..21939
                     /locus_tag="J8I47_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DADOZM010000035        22026 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.035, whole genome shotgun sequence.
ACCESSION   DADOZM010000035 DADOZM010000000
VERSION     DADOZM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.035"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(61..1371)
                     /locus_tag="J8I47_001149"
     CDS             complement(61..1371)
                     /locus_tag="J8I47_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAZ6322599.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="J8I47_001150"
     CDS             complement(1383..2801)
                     /locus_tag="J8I47_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322600.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="J8I47_001151"
     CDS             complement(3041..4609)
                     /locus_tag="J8I47_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAZ6322601.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="J8I47_001152"
     CDS             complement(4613..4894)
                     /locus_tag="J8I47_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAZ6322602.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="J8I47_001153"
     CDS             complement(4891..5814)
                     /locus_tag="J8I47_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAZ6322603.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="J8I47_001154"
     CDS             complement(5811..6689)
                     /locus_tag="J8I47_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAZ6322604.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="J8I47_001155"
     CDS             complement(6676..8013)
                     /locus_tag="J8I47_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAZ6322605.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="J8I47_001156"
     CDS             complement(8016..9119)
                     /locus_tag="J8I47_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAZ6322606.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="J8I47_001157"
     CDS             complement(9193..10701)
                     /locus_tag="J8I47_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6322607.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="J8I47_001158"
     CDS             complement(10667..11359)
                     /locus_tag="J8I47_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ6322608.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="J8I47_001159"
     CDS             complement(11381..12355)
                     /locus_tag="J8I47_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322609.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="J8I47_001160"
     CDS             12655..13527
                     /locus_tag="J8I47_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322610.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="J8I47_001161"
     CDS             complement(14109..16307)
                     /locus_tag="J8I47_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6322611.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="J8I47_001162"
     CDS             16539..17531
                     /locus_tag="J8I47_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6322612.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="J8I47_001163"
     CDS             17591..19012
                     /locus_tag="J8I47_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAZ6322613.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="J8I47_001164"
     CDS             19005..20504
                     /locus_tag="J8I47_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAZ6322614.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="J8I47_001165"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="J8I47_001165"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAZ6322615.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DADOZM010000036        21073 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.036, whole genome shotgun sequence.
ACCESSION   DADOZM010000036 DADOZM010000000
VERSION     DADOZM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.036"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(263..706)
                     /locus_tag="J8I47_001166"
     CDS             complement(263..706)
                     /locus_tag="J8I47_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAZ6322616.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(924..2264)
                     /locus_tag="J8I47_001167"
     CDS             complement(924..2264)
                     /locus_tag="J8I47_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6322617.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2533..3429
                     /locus_tag="J8I47_001168"
     CDS             2533..3429
                     /locus_tag="J8I47_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAZ6322618.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3579..4520)
                     /locus_tag="J8I47_001169"
     CDS             complement(3579..4520)
                     /locus_tag="J8I47_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAZ6322619.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4646..5767
                     /locus_tag="J8I47_001170"
     CDS             4646..5767
                     /locus_tag="J8I47_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ6322620.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            6025..7527
                     /locus_tag="J8I47_001171"
     CDS             6025..7527
                     /locus_tag="J8I47_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAZ6322621.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7594..8121
                     /locus_tag="J8I47_001172"
     CDS             7594..8121
                     /locus_tag="J8I47_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ6322622.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8332..9393
                     /locus_tag="J8I47_001173"
     CDS             8332..9393
                     /locus_tag="J8I47_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAZ6322623.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9396..10292
                     /gene="murQ"
                     /locus_tag="J8I47_001174"
     CDS             9396..10292
                     /gene="murQ"
                     /locus_tag="J8I47_001174"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAZ6322624.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10306..12288
                     /locus_tag="J8I47_001175"
     CDS             10306..12288
                     /locus_tag="J8I47_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6322625.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12353..12799)
                     /gene="nrdI"
                     /locus_tag="J8I47_001176"
     CDS             complement(12353..12799)
                     /gene="nrdI"
                     /locus_tag="J8I47_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAZ6322626.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            13010..14434
                     /locus_tag="J8I47_001177"
     CDS             13010..14434
                     /locus_tag="J8I47_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6322627.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14615..15388)
                     /locus_tag="J8I47_001178"
     CDS             complement(14615..15388)
                     /locus_tag="J8I47_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ6322628.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15385..16290)
                     /locus_tag="J8I47_001179"
     CDS             complement(15385..16290)
                     /locus_tag="J8I47_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322629.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16444..16764)
                     /locus_tag="J8I47_001180"
     CDS             complement(16444..16764)
                     /locus_tag="J8I47_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAZ6322630.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17238..17924
                     /locus_tag="J8I47_001181"
     CDS             17238..17924
                     /locus_tag="J8I47_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAZ6322631.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17926..18870
                     /locus_tag="J8I47_001182"
     CDS             17926..18870
                     /locus_tag="J8I47_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAZ6322632.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            19069..19713
                     /gene="pcp"
                     /locus_tag="J8I47_001183"
     CDS             19069..19713
                     /gene="pcp"
                     /locus_tag="J8I47_001183"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAZ6322633.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19792..21069
                     /locus_tag="J8I47_001184"
     CDS             19792..21069
                     /locus_tag="J8I47_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322634.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6802 a         3804 c         4374 g         6093 t
ORIGIN      
        1 ccatttgtct ctctaatact caggtcagaa cgctgatttt acaacctctt aataaacggt
       61 gttcaaaagc ttgcatctgc ggtaataagc ctaaccaaac gaaaaatagg agaagctatt
      121 tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaaccc cttattcacg
      181 gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga acatcctctt
      241 tttttatttg gacggataat ggtcattcca tttctttgat catttttttc attttgtcga
      301 tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca ggatctttta
      361 aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg gccttcatcc
      421 aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg gcaaaaacaa
      481 gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt ttccacatga
      541 acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat actcgtgtac
      601 gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca aacataacta
      661 ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa aacaaactcc
      721 tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa ttattttgca
      781 cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc ggaaaaagga
      841 gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat tccacataaa
      901 taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc attctttttt
      961 tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac cccaatggag
     1021 ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa aaagctgatt
     1081 cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc actgataatg
     1141 atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa taatttgtca
     1201 ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa taaaacagca
     1261 cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc ttttcgatcc
     1321 ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat agatgagaac
     1381 aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt cattttttca
     1441 aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg aaaaagaatc
     1501 aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac acctttgatt
     1561 gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc aatcagttca
     1621 gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt cataccatga
     1681 ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc tgtgcccaac
     1741 atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac actgatttta
     1801 gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag attgatgatc
     1861 gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg gaaccacaac
     1921 tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca catagaccag
     1981 tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat caaaccagat
     2041 aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt catcacgata
     2101 tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc gatcccaatc
     2161 gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc taacatggtg
     2221 atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga cctctttctt
     2281 ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca atgactaatg
     2341 ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat tttaatagaa
     2401 gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa atataggaaa
     2461 cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt
     2521 gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa atcactttat
     2581 tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa
     2641 ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat
     2701 ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta
     2761 tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
     2821 ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa
     2881 cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat
     2941 ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa
     3001 tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa
     3061 cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg
     3121 gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt
     3181 caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa
     3241 aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca
     3301 tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga
     3361 tttatcacta acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa
     3421 aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct
     3481 tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa
     3541 ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct
     3601 tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt
     3661 tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg
     3721 atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa
     3781 gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa
     3841 gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca
     3901 gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt
     3961 cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag
     4021 acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt
     4081 tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg
     4141 atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat
     4201 ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac
     4261 atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt
     4321 tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag
     4381 tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag
     4441 aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc
     4501 ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat
     4561 atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta
     4621 cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg
     4681 aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg
     4741 aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta
     4801 cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa
     4861 aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca
     4921 agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag
     4981 ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt
     5041 cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc
     5101 ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga
     5161 tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc
     5221 atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac
     5281 gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg
     5341 ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct
     5401 ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt
     5461 ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca
     5521 aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc
     5581 aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc
     5641 aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca
     5701 aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc
     5761 gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg
     5821 agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt
     5881 gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg
     5941 gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     6001 tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc
     6061 tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg
     6121 aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa
     6181 aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa
     6241 cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag
     6301 atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga
     6361 gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg
     6421 gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga
     6481 aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac
     6541 cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa
     6601 ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa
     6661 atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa
     6721 aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc
     6781 gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg
     6841 aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta
     6901 ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag
     6961 cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt
     7021 cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag
     7081 tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac
     7141 cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg
     7201 ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga
     7261 aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa
     7321 cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat
     7381 gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac
     7441 gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact
     7501 gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt
     7561 aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa
     7621 acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa
     7681 ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg
     7741 aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt
     7801 ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat
     7861 ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc
     7921 ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc
     7981 atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc
     8041 aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca
     8101 gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca
     8161 ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc
     8221 aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga
     8281 tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc
     8341 ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg
     8401 gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg
     8461 gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg
     8521 atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg
     8581 cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag
     8641 attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa
     8701 gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat
     8761 tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg
     8821 ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc
     8881 ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc
     8941 gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca
     9001 gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg
     9061 gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac
     9121 gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca
     9181 gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg
     9241 cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat
     9301 gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg
     9361 aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc
     9421 taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag
     9481 ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga
     9541 caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga
     9601 tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac
     9661 cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa
     9721 tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca
     9781 agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat
     9841 atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt
     9901 gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc
     9961 cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga
    10021 atatgatttc tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg
    10081 atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga
    10141 cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc
    10201 tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg
    10261 aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa
    10321 ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta
    10381 gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta
    10441 aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt
    10501 caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca
    10561 ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca
    10621 tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa
    10681 caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg
    10741 attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta
    10801 gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc
    10861 ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt
    10921 tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg
    10981 aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt
    11041 gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat
    11101 aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc
    11161 ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc
    11221 ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca
    11281 ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa
    11341 atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga
    11401 caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa
    11461 atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga
    11521 gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg
    11581 gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct
    11641 ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct
    11701 tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa
    11761 gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa
    11821 acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta
    11881 gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa
    11941 ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg
    12001 aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc
    12061 aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg
    12121 acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa
    12181 acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa
    12241 acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac
    12301 aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat
    12361 tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat
    12421 aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact
    12481 gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt
    12541 catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac
    12601 catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa
    12661 aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat
    12721 gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg
    12781 atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt
    12841 ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa
    12901 aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag
    12961 gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga
    13021 gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat
    13081 tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat
    13141 tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat
    13201 caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg
    13261 ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt
    13321 agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg
    13381 gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt
    13441 gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt
    13501 gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga
    13561 taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca
    13621 agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt
    13681 cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt
    13741 cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa
    13801 tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg
    13861 taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct
    13921 ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc
    13981 taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac
    14041 gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt ataactttgc
    14101 ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat
    14161 gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc
    14221 ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct
    14281 tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca
    14341 acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat
    14401 ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg
    14461 ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt
    14521 cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga
    14581 acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga
    14641 gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag
    14701 tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca
    14761 acatactgat cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac
    14821 cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat gcgccggaac
    14881 tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac
    14941 tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg
    15001 tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa
    15061 ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc
    15121 gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga
    15181 tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag
    15241 cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta
    15301 tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa
    15361 ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat
    15421 ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt
    15481 ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt
    15541 gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag
    15601 atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt
    15661 acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag
    15721 ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat
    15781 gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat
    15841 aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa
    15901 cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt
    15961 gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa
    16021 gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact
    16081 agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc
    16141 taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat
    16201 tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt
    16261 ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac
    16321 aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc
    16381 ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag
    16441 tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa
    16501 gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc
    16561 aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc
    16621 ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat
    16681 tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc
    16741 tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa
    16801 aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt atcctacccg
    16861 gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc
    16921 atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg
    16981 aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta
    17041 actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt accttacaat
    17101 aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata gtgtctattt
    17161 cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat
    17221 tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc
    17281 tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag
    17341 ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa
    17401 acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa
    17461 atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta
    17521 tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg
    17581 gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat
    17641 caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta
    17701 tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc
    17761 tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag
    17821 tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag
    17881 aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca
    17941 aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc
    18001 gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg
    18061 tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca
    18121 atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc
    18181 aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga
    18241 atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt
    18301 accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg
    18361 atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg
    18421 ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt
    18481 acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga
    18541 aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg
    18601 gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt
    18661 agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg
    18721 accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg
    18781 aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc
    18841 caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac agaataattg
    18901 tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt
    18961 gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta
    19021 tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta
    19081 gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa
    19141 aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc
    19201 gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc
    19261 tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta
    19321 gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa
    19381 aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa
    19441 gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc
    19501 atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc
    19561 cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct
    19621 ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa
    19681 gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct
    19741 aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa
    19801 tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa
    19861 atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa
    19921 cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa
    19981 cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga
    20041 acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac
    20101 atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat
    20161 cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat
    20221 ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta
    20281 tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg
    20341 gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata
    20401 gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag
    20461 tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag
    20521 gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc
    20581 gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg
    20641 agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa
    20701 aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat
    20761 ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa
    20821 gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca
    20881 aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc
    20941 gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat
    21001 tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa
    21061 ttactataaa aaa
//
LOCUS       DADOZM010000037        20485 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.037, whole genome shotgun sequence.
ACCESSION   DADOZM010000037 DADOZM010000000
VERSION     DADOZM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.037"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="J8I47_001185"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="J8I47_001185"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAZ6322635.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="J8I47_001186"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="J8I47_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAZ6322636.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="J8I47_001187"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="J8I47_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAZ6322637.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="J8I47_001188"
     CDS             2856..3731
                     /locus_tag="J8I47_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAZ6322638.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="J8I47_001189"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="J8I47_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAZ6322639.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="J8I47_001190"
     CDS             4628..5062
                     /locus_tag="J8I47_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAZ6322640.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="J8I47_001191"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="J8I47_001191"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAZ6322641.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="J8I47_001192"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="J8I47_001192"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAZ6322642.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="J8I47_001193"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="J8I47_001193"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAZ6322643.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="J8I47_001194"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="J8I47_001194"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAZ6322644.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="J8I47_001195"
     CDS             13333..14259
                     /locus_tag="J8I47_001195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAZ6322645.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="J8I47_001196"
     CDS             14441..15334
                     /locus_tag="J8I47_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAZ6322646.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="J8I47_001197"
     CDS             15468..15593
                     /locus_tag="J8I47_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6322647.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="J8I47_001198"
     CDS             complement(15727..16359)
                     /locus_tag="J8I47_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322648.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="J8I47_001199"
     CDS             complement(16507..17049)
                     /locus_tag="J8I47_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAZ6322649.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="J8I47_001200"
     CDS             17184..17573
                     /locus_tag="J8I47_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322650.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="J8I47_001201"
     CDS             17577..18341
                     /locus_tag="J8I47_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ6322651.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="J8I47_001202"
     CDS             18470..19417
                     /locus_tag="J8I47_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6322652.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="J8I47_001203"
     CDS             19547..20287
                     /locus_tag="J8I47_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322653.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7191 a         3481 c         4316 g         5497 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttataat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agttt
//
LOCUS       DADOZM010000038        20432 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.038, whole genome shotgun sequence.
ACCESSION   DADOZM010000038 DADOZM010000000
VERSION     DADOZM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.038"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            297..1406
                     /locus_tag="J8I47_001204"
     CDS             297..1406
                     /locus_tag="J8I47_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322654.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="J8I47_001205"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="J8I47_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="J8I47_001206"
     CDS             2401..3225
                     /locus_tag="J8I47_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322655.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="J8I47_001207"
     CDS             3257..4567
                     /locus_tag="J8I47_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAZ6322656.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="J8I47_001208"
     CDS             4715..5974
                     /locus_tag="J8I47_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6322657.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="J8I47_001209"
     CDS             5984..6856
                     /locus_tag="J8I47_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322658.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="J8I47_001210"
     CDS             6868..7698
                     /locus_tag="J8I47_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322659.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="J8I47_001211"
     CDS             7738..9921
                     /locus_tag="J8I47_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAZ6322660.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="J8I47_001212"
     CDS             9982..11202
                     /locus_tag="J8I47_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAZ6322661.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="J8I47_001213"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="J8I47_001213"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAZ6322662.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="J8I47_001214"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="J8I47_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="J8I47_001215"
     CDS             14164..14373
                     /locus_tag="J8I47_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ6322663.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="J8I47_001216"
     CDS             14366..14515
                     /locus_tag="J8I47_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAZ6322664.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="J8I47_001217"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="J8I47_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="J8I47_001218"
     CDS             complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="J8I47_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAZ6322665.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17037..17270
                     /locus_tag="J8I47_001219"
     CDS             17037..17270
                     /locus_tag="J8I47_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAZ6322666.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17267..17674
                     /locus_tag="J8I47_001220"
     CDS             17267..17674
                     /locus_tag="J8I47_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAZ6322667.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18811..19329)
                     /locus_tag="J8I47_001221"
     CDS             complement(18811..19329)
                     /locus_tag="J8I47_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAZ6322668.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         6931 a         3148 c         3803 g         6550 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
    16621 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
    16681 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
    16741 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
    16801 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
    16861 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
    16921 ttaattatat agaaagaaca atctttctat agagttagac atgagaggga ctatgcttga
    16981 tttaaaatca acaagcgtat atactgtata tacaaagaaa aaggagggag aagactatgg
    17041 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
    17101 agtttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
    17161 ttcgagcggc aattacaaaa aaacaagcct tatctgatga aattgattta gcaatatctc
    17221 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
    17281 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
    17341 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
    17401 ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta tcctacttat
    17461 acaattgata aagaaacatc cttttcacaa tgcaaataaa cgtactgctt tcttagctac
    17521 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
    17581 tttagcggta tatattgcta cgtatgataa ggatttcgat caactaaaat atgaagtgat
    17641 caacactata aagacacata cgcagcccat ttaaggagtt actgtatgtg tctttatagg
    17701 gttattagct gctatagcga aataattgaa gtaaattaca aatagctata attcaaatcc
    17761 aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
    17821 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
    17881 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
    17941 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
    18001 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
    18061 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt
    18121 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
    18181 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
    18241 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
    18301 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
    18361 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
    18421 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
    18481 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
    18541 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
    18601 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
    18661 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
    18721 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
    18781 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
    18841 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
    18901 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
    18961 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
    19021 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
    19081 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
    19141 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
    19201 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
    19261 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
    19321 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
    19381 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
    19441 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
    19501 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
    19561 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
    19621 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
    19681 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
    19741 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
    19801 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
    19861 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
    19921 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
    19981 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
    20041 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
    20101 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
    20161 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
    20221 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
    20281 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
    20341 atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac tagacagaaa
    20401 aattaaggtg tgtagactag aagaaaacat ac
//
LOCUS       DADOZM010000039        20217 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.040, whole genome shotgun sequence.
ACCESSION   DADOZM010000039 DADOZM010000000
VERSION     DADOZM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.040"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..152
                     /locus_tag="J8I47_001222"
     CDS             <1..152
                     /locus_tag="J8I47_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6322669.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="J8I47_001223"
     CDS             complement(230..763)
                     /locus_tag="J8I47_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAZ6322670.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="J8I47_001224"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="J8I47_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAZ6322671.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="J8I47_001225"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="J8I47_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAZ6322672.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="J8I47_001226"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="J8I47_001226"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAZ6322673.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="J8I47_001227"
     CDS             complement(4488..5066)
                     /locus_tag="J8I47_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAZ6322674.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="J8I47_001228"
     CDS             5388..5858
                     /locus_tag="J8I47_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAZ6322675.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="J8I47_001229"
     CDS             complement(5966..7192)
                     /locus_tag="J8I47_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAZ6322676.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="J8I47_001230"
     CDS             complement(7946..8146)
                     /locus_tag="J8I47_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6322677.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="J8I47_001231"
     CDS             complement(8280..8888)
                     /locus_tag="J8I47_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAZ6322678.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="J8I47_001232"
     CDS             complement(8973..9443)
                     /locus_tag="J8I47_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAZ6322679.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="J8I47_001233"
     CDS             complement(9491..10405)
                     /locus_tag="J8I47_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAZ6322680.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="J8I47_001234"
     CDS             10551..11354
                     /locus_tag="J8I47_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAZ6322681.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="J8I47_001235"
     CDS             11532..12479
                     /locus_tag="J8I47_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAZ6322682.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="J8I47_001236"
     CDS             complement(12547..12840)
                     /locus_tag="J8I47_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAZ6322683.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="J8I47_001237"
     CDS             complement(12868..13533)
                     /locus_tag="J8I47_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAZ6322684.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="J8I47_001238"
     CDS             complement(13673..14305)
                     /locus_tag="J8I47_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322685.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="J8I47_001239"
     CDS             complement(14312..15379)
                     /locus_tag="J8I47_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6322686.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="J8I47_001240"
     CDS             complement(15376..16107)
                     /locus_tag="J8I47_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAZ6322687.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="J8I47_001241"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="J8I47_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAZ6322688.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="J8I47_001242"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="J8I47_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAZ6322689.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="J8I47_001243"
     CDS             18270..19439
                     /locus_tag="J8I47_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAZ6322690.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20217)
                     /locus_tag="J8I47_001244"
     CDS             complement(19706..>20217)
                     /locus_tag="J8I47_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322691.1"
                     /translation="LLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDY
                     VPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLT
                     VTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCI
                     KLKIRTSHE"
BASE COUNT         6125 a         4026 c         3213 g         6853 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataata
//
LOCUS       DADOZM010000040        20114 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.041, whole genome shotgun sequence.
ACCESSION   DADOZM010000040 DADOZM010000000
VERSION     DADOZM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.041"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            4..360
                     /locus_tag="J8I47_001245"
     CDS             4..360
                     /locus_tag="J8I47_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAZ6322692.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            320..565
                     /locus_tag="J8I47_001246"
     CDS             320..565
                     /locus_tag="J8I47_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322693.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            562..981
                     /locus_tag="J8I47_001247"
     CDS             562..981
                     /locus_tag="J8I47_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAZ6322694.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            978..1535
                     /locus_tag="J8I47_001248"
     CDS             978..1535
                     /locus_tag="J8I47_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAZ6322695.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1532..1828
                     /locus_tag="J8I47_001249"
     CDS             1532..1828
                     /locus_tag="J8I47_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322696.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1905..2318
                     /locus_tag="J8I47_001250"
     CDS             1905..2318
                     /locus_tag="J8I47_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAZ6322697.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            2664..3038
                     /locus_tag="J8I47_001251"
     CDS             2664..3038
                     /locus_tag="J8I47_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322698.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            3268..3612
                     /locus_tag="J8I47_001252"
     CDS             3268..3612
                     /locus_tag="J8I47_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ6322699.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3617..3898
                     /locus_tag="J8I47_001253"
     CDS             3617..3898
                     /locus_tag="J8I47_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322700.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4001..4315
                     /locus_tag="J8I47_001254"
     CDS             4001..4315
                     /locus_tag="J8I47_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAZ6322701.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            4293..5987
                     /locus_tag="J8I47_001255"
     CDS             4293..5987
                     /locus_tag="J8I47_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ6322702.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            6007..7185
                     /locus_tag="J8I47_001256"
     CDS             6007..7185
                     /locus_tag="J8I47_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ6322703.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7148..7834
                     /locus_tag="J8I47_001257"
     CDS             7148..7834
                     /locus_tag="J8I47_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAZ6322704.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7834..8994
                     /locus_tag="J8I47_001258"
     CDS             7834..8994
                     /locus_tag="J8I47_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ6322705.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            9004..9879
                     /locus_tag="J8I47_001259"
     CDS             9004..9879
                     /locus_tag="J8I47_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322706.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9876..10187
                     /locus_tag="J8I47_001260"
     CDS             9876..10187
                     /locus_tag="J8I47_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322707.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10177..10530
                     /locus_tag="J8I47_001261"
     CDS             10177..10530
                     /locus_tag="J8I47_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAZ6322708.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10520..10921
                     /locus_tag="J8I47_001262"
     CDS             10520..10921
                     /locus_tag="J8I47_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322709.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10914..11318
                     /locus_tag="J8I47_001263"
     CDS             10914..11318
                     /locus_tag="J8I47_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322710.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            11330..11938
                     /locus_tag="J8I47_001264"
     CDS             11330..11938
                     /locus_tag="J8I47_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ6322711.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            11958..12320
                     /locus_tag="J8I47_001265"
     CDS             11958..12320
                     /locus_tag="J8I47_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322712.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            12323..12505
                     /locus_tag="J8I47_001266"
     CDS             12323..12505
                     /locus_tag="J8I47_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322713.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12522..15941
                     /locus_tag="J8I47_001267"
     CDS             12522..15941
                     /locus_tag="J8I47_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAZ6322714.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15992..16729
                     /locus_tag="J8I47_001268"
     CDS             15992..16729
                     /locus_tag="J8I47_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ6322715.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            16739..19030
                     /locus_tag="J8I47_001269"
     CDS             16739..19030
                     /locus_tag="J8I47_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322716.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            19054..>20114
                     /locus_tag="J8I47_001270"
     CDS             19054..>20114
                     /locus_tag="J8I47_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322717.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
BASE COUNT         7192 a         2936 c         4276 g         5710 t
ORIGIN      
        1 ataatggatc tcattacaca atacagtgac atcatcctca agaaaatcat gatgaagatt
       61 cagaaagata aaaaatcaaa agaacgagct gaattagtta agttagaaat ggctgaaaca
      121 ggagcaggag tgcgaagtag caggcattgg aaagcagcag caaacattga attttattac
      181 aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac aaattggagc
      241 aagaaacttc atcaagatcg tttcaaattt gtagagaagt ataaagaaat attagaagag
      301 tatttgagga ggacagcaaa tgataaaaaa actcgttcaa ttcagcatgg atttatatga
      361 tatcgaatca ggagcaacac tatctgtgga atcggaccat ctaatcataa attttggtgg
      421 aaagcgccag attattttgt gggtagttga tgatgtactg tttccagaaa ttgttcatga
      481 tttcgaagaa tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg aattgattga
      541 aaaatacgag gaggacagcg aatgataccg aagtttagag cgtgggataa acgaaagaac
      601 gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa ctttattgaa
      661 tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga cctcatgcaa
      721 tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat agtattagtc
      781 agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa aaattcaata
      841 gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca aatttttaac
      901 acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga gaatagcgag
      961 ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa agtattaaag
     1021 cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca ggtattacgt
     1081 taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg gccggttggc
     1141 ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta atcaaatcta
     1201 aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata gtgacgcaag
     1261 agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca ctattgagcc
     1321 tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat gccgatggaa
     1381 actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt tttgaatgca
     1441 atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca gcattgttgg
     1501 tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat tttacgctag
     1561 cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta aaacacgcaa
     1621 gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt tcgtttatcc
     1681 gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta ttgaaaatac
     1741 aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag cgagattagt
     1801 tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact gattcaataa
     1861 atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa ttgttacgag
     1921 aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac tttcggcgtt
     1981 tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt acggatatgc
     2041 cgaaggcacc aaagcacggc aataaggcag aagacgcgat cattcagatg atggatatag
     2101 aagcggagag agatgcgatt ctagcggctt tgatggctct tagtctaatt agtcgccaga
     2161 tactctacta cagcttctgt gtgccagata gcttctcaaa ctacaggatt agccgtgaag
     2221 tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata gagtttgcag
     2281 aagcatataa acacggaaga ataattgctt ataaataatt tggcggtttt ttggcggaat
     2341 gatggcggtt tttagctatt taccagtgat attatggtag tgtcgaaaga ttagtgatag
     2401 gtctaagaca aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga
     2461 tagacagcag caaataaact aaaggatgtg ggggtcagct cctacagtta gttcatgtgt
     2521 tgctgtctat taattgcaac tctttcggtt ttattgagta tttactcaac ttaaaaaaac
     2581 tgttgtttgt agatgtaagc tgttcctatt tgtacatagt aaaaaatgat ataataaaaa
     2641 tattacgaaa ggggtgagag tgcatggaag acaaaatgta tttaagacaa aaagttttca
     2701 caaaaaaaga tattgaaaat caaattaatg gatttataca gaaattagaa aatgcaaatt
     2761 cttatttgat aaataaggaa ctaaataaag cttatgacca gtggaaagaa gtatatgatg
     2821 agttgaaatt gattcaaaat gaaacaaaat tagtacgaac ggagaaaaag aatgaaaata
     2881 gtttcttttt tgatggatat gctatattga tgttggaaac tgtagcaaaa caaaatataa
     2941 aagctcccaa aaaagaacta tctgacaata ttgataacgc attagccgag ttaaggtatt
     3001 acgttatgca aataaaagat gttaggatca ctcgttgagt ggtcttttta ttttgcacaa
     3061 aggaggtaac aacaatgtat agaccacaat acttagaaca gaagtatgaa gtaatcactg
     3121 tgcaaaatgg taacggagag atagtacgaa agtatagaag accaataaag agcgatacat
     3181 ataaacgaaa ggaaagcaat gaagttattc cattgtatgg caaaagaata gctaagcatt
     3241 aaataagatt gtgaaaggag acgggacatg aacgaggaat tctatagatg gctattacag
     3301 ttgataagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg caggcttaga
     3361 gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact aggtaagtat
     3421 catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga cttagcttta
     3481 gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca tggcagatat
     3541 cttacagcat tggataaaca agagaagaag atagaaagct ttgctaactt cgatgcaagt
     3601 gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata cagaaaagat
     3661 tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg ttcgctatgt
     3721 aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga atcaagtaag
     3781 agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg gatatagtat
     3841 tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg tgagataacc
     3901 cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg ctgtcaggaa
     3961 aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa acgaattgtc
     4021 tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg aaagtcagaa
     4081 aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat gggaaacggc
     4141 gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg gtaaaaagaa
     4201 tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt tggataaact
     4261 tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt tataagcata
     4321 ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa gagcaattat
     4381 tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac ttcgataagc
     4441 agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa ttaattccgt
     4501 gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat aaaacactag
     4561 tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt tttgcttctt
     4621 ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat attgatatct
     4681 atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa gtaattaaag
     4741 atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt caaaatatcg
     4801 ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat gggaagcgac
     4861 caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg
     4921 ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca actaatggtc
     4981 atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt ctttctggag
     5041 aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt atttgtcgct
     5101 tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt ccaactatta
     5161 attacaatgc aaatttaaaa cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg
     5221 ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa gatacacgat
     5281 ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa
     5341 tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc agcgttgggt
     5401 tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc cacgaatcag
     5461 cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat caaggaaaat
     5521 cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg tttcttgaaa
     5581 tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca aaaatattga
     5641 agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga tctgttacac
     5701 atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta ttttttggtg
     5761 acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt tctaatggaa
     5821 atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc
     5881 ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca gtcacaaata
     5941 gctcagtaga aaatacagga actggattta ctcctctagt attctaactt gaaaggaggt
     6001 gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc aacggttcct
     6061 ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa
     6121 acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc atatgaagat
     6181 ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa taagaaaaat
     6241 aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa tgaagtattg
     6301 atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg ggatgtcaca
     6361 gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat agtccttgat
     6421 agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca aattaggcaa
     6481 ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat gaatgtttac
     6541 aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc gcaagacaat
     6601 acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt tatggaagct
     6661 gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga tttcagcgga
     6721 aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt tgagatgaca
     6781 gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg gttatcggat
     6841 caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat tcaggatgcg
     6901 tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt acgtgtggat
     6961 acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga tgtaggcatt
     7021 agaaatggag tatttacaat caacgaagga agagaacgcg ttggaaatga tcgctctgat
     7081 aaggcgatgg cagatgaaat atttataact aaaaacaatc aacaagtatc gaaaggaggt
     7141 gaggcgaatg acgacaatga aaacatttct agcagtaaag aatgaaggcg cagtaccgca
     7201 aatttttatt cagggattta ttggttctag ttggttcttt gaagggaata ctgacaaggg
     7261 aatcaaaaat attttggata gtctaggtga tcaagaagaa attgaagtag taattaattc
     7321 aaacggtgga gacgtatttc aagggattgc tattgggaac ttacttaagt caaataaagc
     7381 aaaagttaac gttgtgatta acggcttagc cgctagtgct gcttcaatta tcgcaatggc
     7441 tggcgatact ataaaaatct acaacaatgc acaattgatg attcaccgcg cttccacata
     7501 cggagaagga aatgtcgatg acttccgtac gattgctgac caactggaat caattgataa
     7561 atcggtaaag gcttcatata aaacacgatt caatggcaca gatgaagcat tgcaagaact
     7621 tcttgaaaaa gaatcgttta tggatgcaga aacagctttg agttatggat tggtcgatga
     7681 aattatcgat gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa gcgttgaaga
     7741 aattttgaat gacgttaaag aaaaaagagc agaaaaaatt gctgcattta cagcagcatt
     7801 aaataaaaca tttggacaag gagatgtaaa ataatgacag ttaaaaattt aaaaggtgta
     7861 acagctgcaa gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga atcttttagc
     7921 gcagctatgg taagcttatc taaggaaatt caggataaaa ttttagaaga agcaacagca
     7981 aaaaatcaag atcaattagt attaatgaac cgtggtcagc gtgtattaac tacacaagaa
     8041 acaaaattct ataacgaagt agtgaaaaac gaaggttttg caggggtcga agaattagtg
     8101 ccagctactg tatttgaacg tgtatttgaa gatttagaac aatctcatcc actattgcaa
     8161 aaaattactt ttgttaacac aactggtgta acagaatgga ttgtgtcacg tggagtcaat
     8221 ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag ttttagataa tggctttgat
     8281 gtaattaaca tgaagcagtt caagctatca ggttatattc ctgtatgtaa ggcaatgctt
     8341 gatttaggtc cagtatggtt agatcgttat gtccgtactg ttttagtaga atcattgaga
     8401 attgcattag aacaagcaat tgttgatggt actggtaaag atatgccagt cggaatgatg
     8461 cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga acctattaca
     8521 gctttagatg ctgtaactat gggccgtttg atggcgcgac tatcaaaatt caatatcgaa
     8581 ggtgtgaatg atccgattta tcgtaatgtg aatccttctg atgtggtcct aattgtgaat
     8641 ccaactgatt actggtctaa agtattccca gctaagactg tactaactgc taatggagaa
     8701 tacgtacaag tattgccagt accagtttca gatttgcagt caacggctgt gccagaagga
     8761 aaagcagtta ttggggtagc ctcagattac ttcatgggtg taggatctac actaaaaatt
     8821 gaagcttcag atgaatacca ttttgttgaa gacgaacgca tttatctagc taaacaatat
     8881 gcaaacggtc aacctaaacg taacgatagt ttcattgtat tagatattag cgctttggga
     8941 actactacta caactacaaa accaacaacc acaacaacta caacacaagc gtaggtgatc
     9001 agaatgaagt atattctttg tcagccggca atcaatcggt ttaaatggga gcttgaagtt
     9061 tgtttaacta atctgaagaa actaggaatc aaagatatcg tattgctttt cagcagacac
     9121 gatgatcaga ttcctatttt ttttgagaag gaatatggtg ttgaagttca tgtgtacgat
     9181 gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg gtggaaatat
     9241 ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc ggatgtcatt
     9301 ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg gtattgtagc
     9361 gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg agaaaatatt
     9421 ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac tataaacact
     9481 aattcaggag gcgcacagtg ggttattaac cgtcctaaag cgaattattg gaaaaaggtt
     9541 tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa tatacaaatc
     9601 tggacagccg agatgtgggc acggctttgg aacatgatgt atttcaatat tggtcctaaa
     9661 gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt taaagaagta
     9721 aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt caaagggaga
     9781 tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa aaaatgctct
     9841 tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa gtgactgaag
     9901 aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa gatgagtcaa
     9961 ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt acaacacttt
    10021 ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga tacgactgga
    10081 atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt ttcattcaac
    10141 attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg tagactcgaa
    10201 gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta
    10261 ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag aaatctttcc
    10321 atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac taagaaagta
    10381 aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt tatcgtaatc
    10441 gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat ctatttttat
    10501 cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac gtttaaataa
    10561 gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac tctttcaaga
    10621 ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg aaacaggaga
    10681 aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc taactttcta
    10741 ttcagaaaat agagaagatt taacaggaga ctcactagat attatttcat tgattcaaaa
    10801 tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga accaagatcg
    10861 ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt gtcatggcta
    10921 aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg atggaacgct
    10981 attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt tcggctattg
    11041 cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga agagggcacc
    11101 aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt ttcatcatta
    11161 gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt acttctaaaa
    11221 gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat ccaattacag
    11281 aacttctgat tcgtcaattc gataaattaa ataaataggg ggaacaacaa tggctaaaac
    11341 aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt ttaattttaa
    11401 gaacgcagga aatgcaatcg caacgaattg taatggacaa ttagatggcg aaacagaaat
    11461 gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa tcaaaatcta aaccaatcaa
    11521 tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgtt tcaatgggtt
    11581 gaaacaagat ggacgtatta aaccaggcat ttactcttac ggtcctgatt ccgtaggcga
    11641 agatttctca cttgctgcag agatcgtgga tgacttcgaa gaaaatagaa agttagttgg
    11701 tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga
    11761 agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca
    11821 tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc
    11881 tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa
    11941 aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa
    12001 ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta
    12061 acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc
    12121 aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat
    12181 tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat
    12241 tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg
    12301 aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg tcgatgtcta
    12361 ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt
    12421 agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc
    12481 aataaaccaa tctcagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag
    12541 aagcagaagt aactttcata gctaacgatg acggattgaa atctacgtta aaagaaatca
    12601 gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga
    12661 ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat
    12721 tacaaagcca aaaagttgaa gtaactagcc aacgtttagc caatgccaaa aaatattatg
    12781 gagaaaattc caccgaagtt cagaaacttg agagagaact gattaaccaa caaacagcgc
    12841 aacaacgttt gtcaaacgaa attgataaaa cgagtaatac actagctcaa gcaaaaggcg
    12901 aaatacagac gtacgagtct acaatgcaac agttggatag tgaacaaaaa aatgttcaag
    12961 ctagtgcttc tctgattgaa tccgaataca aaaaatggca agtaactgct ggtcaatcag
    13021 cttctgaatc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg
    13081 cagagaaaac gatagatatc ctgagacgac agttagaagc tacacagtct gagtttggcg
    13141 ctacatccac agaagcaatg cagatggagg cgaagcttaa tgatgctgaa cgtgaatttg
    13201 aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca
    13261 aaatagatat gaataattta atggaagctt ctgacgtttt aagcgacatt ggcgataagc
    13321 ttacagaatt agggaaacaa gcagtggact ctgctaacag tgtaggtagt tcccagagta
    13381 aaatacaagc taattttggt ttgactaaac aagaggctga agaattaacg aatgtagcca
    13441 gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt
    13501 tggtaaagcg taatttaggc gatttaaata atcaagattt acaaaatatt acggaacaag
    13561 ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg
    13621 gcttaatggt caacttcgga ttgagcgctc aagatgcaat ggatttaatg gtttcgggta
    13681 ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat
    13741 tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag
    13801 atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt
    13861 taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt
    13921 ttgaagagtg gaaaaatggt ggaggaacac aaaaagacgt tattaattcc atgattcaag
    13981 attttagcaa tatggatggt caatacgacc aattaaataa agcttctaca atttggtctg
    14041 cacttggcga agataacgcg atgaaagttg tccaatcttt gactgatgtt aaccatacat
    14101 ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttacaag
    14161 aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca
    14221 tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc
    14281 ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct gcatttactt
    14341 tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca ttaataatag
    14401 gagcagttat tactgctatt gctggagtta tagcaattat aaaaaactgg ggcgcaatta
    14461 ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt acttgggaaa
    14521 gcataaaaga aggtgcctca tcagtttggg atggagtcaa agaaacctgg tctggatttg
    14581 tagattgggt tcaagaaatt tggcaaggag tttctgattg gtttggagag ttatggagcg
    14641 gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg caagcattcg
    14701 ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag
    14761 acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt caagctggta
    14821 ttaatattat cgtgggtatc tttgatgtag ctggacagtt attaggcgca gcttggcaag
    14881 ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg acacaatgga
    14941 taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat ccagtagtgg
    15001 catggtggaa tggtatatgg acatctatta gtactacggc ttcaaatatt tggaactcaa
    15061 ttagtgcaac agcttctagt atttggaata gtatcaagaa tacaatcact agcttggtac
    15121 aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa
    15181 tttggaattc aatcagctct acagcatcaa atatctggag tagcgtgact agtagtataa
    15241 gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt
    15301 ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg aatggaatta
    15361 caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca
    15421 atctaacagg tattgtttcc gacatattca ataatgttaa aagcacgata gctaatatat
    15481 gggaaggtat caaaaagact gttagcgctc cgattgattg gattagagat aaaatcagta
    15541 gtgtctttga taatttgaat atttctatac cacatattcc gttaccacat tttaaattga
    15601 gcggagaatt caatccattg aaggggaaaa tcccaacatt gggtgttgat tggtatgcga
    15661 aaggtagtgt gtttaattct ccaaatatta tcggtgtagg tgaatcagga cctgaagcag
    15721 ttttaccttt gaaaagatct gtgctgcaag aaattggtga tcgtatcttg agtagcacct
    15781 cagtttcatc taggacacaa gcggttcaac ctgtgaacaa ttacgaattc aatttcacaa
    15841 ttgatggtaa cgcagatgaa gtcactatga agcaaacaac tcaacaaatc attgatagca
    15901 ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct
    15961 gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga
    16021 tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc
    16081 aaaaagacag agagttgaaa cttctgtttt aggaaggtta ggtggcttag tgcaagatta
    16141 ctcgtttgaa gacatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca
    16201 tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaactatg caaagaaatt
    16261 agaattctct gacgatccca acgtctatta cgttatccag actatcgata ttggggatgc
    16321 agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc
    16381 aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa
    16441 taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca
    16501 gttcatctta aatgattatg tgtttagttt cgaaggctta gtagcaggaa ctgatgtagt
    16561 cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg
    16621 gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatca atcattttaa
    16681 ttgcactggt ttgagcaaaa tacaaattta tcgtaacggg ttaaggtagg tgaaatagat
    16741 gatcgataat ttaataacta tttacgataa aaatgacgcg aataatttag ctgaacattt
    16801 atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa
    16861 actaaacgga gccgagatat ttcagggtac ttatccaata agcggaacta atgcagattt
    16921 gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct
    16981 acggatttat tatgcaaaaa cttctgtaat aggcaatacg atagaagtaa aagctgaacc
    17041 tattttcaat gatataagaa aatcggtgtt gaataaatat gacagcggaa cagaaaaaat
    17101 cactgctact caggcatggc aaaacgcaaa agctttagcg aaaccagcta tcccttcgca
    17161 gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa aagcgaattt
    17221 tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg gggaatttct
    17281 taaagataat aacacattac atcatgaaaa aaggctaggt acggatcata aaatcaaagc
    17341 gatttatact aaaaatttaa ctggtcttga cttagagata gatgctcaaa gcgttttagt
    17401 tggagtttat ccattcatta gcagttcttc agaaggagaa gacgagatca ctctaccaga
    17461 agaagtcatt ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga
    17521 ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa
    17581 aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag
    17641 gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt
    17701 agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt
    17761 taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt
    17821 agaaaacaca gagaataatg taagcaattt gattaatgat gccattgatc aattgaaaaa
    17881 tggtggcgaa atcagtgatt taatcaatga tattgtagat catcaaactg atatgattac
    17941 tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc gtattttgat
    18001 tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca atgctggtat
    18061 tggtttttct aaaactggca ttaatggaac atatgaaacc gcatggacat tagatggcgg
    18121 attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta gaggtactac
    18181 attaattagt gatggcactg attatagaac aagtattgct aatggcaaaa tgacttggta
    18241 ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga
    18301 tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca
    18361 gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc tatcttttgg
    18421 tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg gtagttcttt
    18481 aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta ggactatgaa
    18541 atttactagt gatggtatgc taacgttgcc aggttgtttt tttggttcat gggaagatgg
    18601 gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg
    18661 tactaaaaac tcaaccgtac caacagaaca ttatggacaa cgactattaa acgcttatga
    18721 aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgatg atggtaaagt
    18781 tcgtgttgat attgacccca tgtttgctga gacagtaaat ctaagtcgat atatgacaca
    18841 tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat
    18901 cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg
    18961 gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa
    19021 acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct
    19081 ctagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa
    19141 gggattacag tgactgttaa accattttta aatggcttgc agatggatac gagtggcgga
    19201 acatttactt taaaaggaac aacgccatct aaccgttacg tagatagtgt ggcaactagt
    19261 gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga agcaggatat
    19321 tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac gcaagatatc
    19381 atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga atacgtttcg
    19441 caattggaag agttgattca aaagtataac gaaacttttg atgcttttat ggctgaaatc
    19501 gaaggtagag tggacagctt aaataaacag attactgatt taactggtca agctaaaaca
    19561 ctacaagaca agttagacgc tctgaaagaa gaaatttcta aattaggtaa cttgcaggtg
    19621 atgtacagta acagcatcga cttcggggga tatgattata gcggtaagcc gaatttaatg
    19681 tccaaactaa aatcgagcga ttttaacgtt ggttaccacg ggtcactaac ttcggataac
    19741 gaaaagctac attttacttc tgatggtaca ggaagcatta ttatgtttac gcgtattaat
    19801 acacctcagc ttgctagtgg gaaaacctat actctgagtg cgaaagttcg atttgatgaa
    19861 ggaactacag gagctattga taaattacgt ttggtgtatc gtacatcacc aggagaaaag
    19921 atattattgg aagcaaatag tacaaatatt acaacagatg atgtagggaa agaaataaca
    19981 atcaaaggta cagctaacgt taattatcaa atcacaaatt tagatcgatt ttatatgagt
    20041 attagctttg ttgacaggga taaaataaat ggcggattta agttgtacga catcaaaatc
    20101 gaagaaggct caac
//
LOCUS       DADOZM010000041        19843 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.042, whole genome shotgun sequence.
ACCESSION   DADOZM010000041 DADOZM010000000
VERSION     DADOZM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.042"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(13..996)
                     /locus_tag="J8I47_001271"
     CDS             complement(13..996)
                     /locus_tag="J8I47_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAZ6322718.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="J8I47_001272"
     CDS             complement(1001..1648)
                     /locus_tag="J8I47_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6322719.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="J8I47_001273"
     CDS             complement(1733..2365)
                     /locus_tag="J8I47_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ6322720.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="J8I47_001274"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="J8I47_001274"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAZ6322721.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="J8I47_001275"
     CDS             complement(2929..3666)
                     /locus_tag="J8I47_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAZ6322722.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="J8I47_001276"
     CDS             complement(3663..4652)
                     /locus_tag="J8I47_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAZ6322723.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="J8I47_001277"
     CDS             4910..5452
                     /locus_tag="J8I47_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6322724.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="J8I47_001278"
     CDS             5469..6554
                     /locus_tag="J8I47_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322725.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="J8I47_001279"
     CDS             6566..7372
                     /locus_tag="J8I47_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322726.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="J8I47_001280"
     CDS             7373..8200
                     /locus_tag="J8I47_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322727.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="J8I47_001281"
     CDS             8197..9270
                     /locus_tag="J8I47_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322728.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="J8I47_001282"
     CDS             9698..12394
                     /locus_tag="J8I47_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6322729.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="J8I47_001283"
     CDS             12471..12959
                     /locus_tag="J8I47_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6322730.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="J8I47_001284"
     CDS             12971..13465
                     /locus_tag="J8I47_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6322731.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="J8I47_001285"
     CDS             13486..14295
                     /locus_tag="J8I47_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6322732.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="J8I47_001286"
     CDS             14292..15113
                     /locus_tag="J8I47_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6322733.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="J8I47_001287"
     CDS             15164..17263
                     /locus_tag="J8I47_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAZ6322734.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="J8I47_001288"
     CDS             17260..18378
                     /locus_tag="J8I47_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAZ6322735.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="J8I47_001289"
     CDS             18417..19295
                     /locus_tag="J8I47_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6322736.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19486..19638
                     /locus_tag="J8I47_001290"
     CDS             19486..19638
                     /locus_tag="J8I47_001290"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322737.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6056 a         3447 c         4246 g         6094 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ttgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggaatt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaa
//
LOCUS       DADOZM010000042        19483 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.043, whole genome shotgun sequence.
ACCESSION   DADOZM010000042 DADOZM010000000
VERSION     DADOZM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.043"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(32..418)
                     /locus_tag="J8I47_001291"
     CDS             complement(32..418)
                     /locus_tag="J8I47_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAZ6322738.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(474..1097)
                     /locus_tag="J8I47_001292"
     CDS             complement(474..1097)
                     /locus_tag="J8I47_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAZ6322739.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(1161..1829)
                     /locus_tag="J8I47_001293"
     CDS             complement(1161..1829)
                     /locus_tag="J8I47_001293"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAZ6322740.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(1841..2281)
                     /locus_tag="J8I47_001294"
     CDS             complement(1841..2281)
                     /locus_tag="J8I47_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322741.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(2340..3012)
                     /locus_tag="J8I47_001295"
                     /pseudo
     CDS             complement(2340..3012)
                     /locus_tag="J8I47_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(3197..3595)
                     /locus_tag="J8I47_001296"
     CDS             complement(3197..3595)
                     /locus_tag="J8I47_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAZ6322742.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(3592..4365)
                     /locus_tag="J8I47_001297"
     CDS             complement(3592..4365)
                     /locus_tag="J8I47_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAZ6322743.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(4362..5045)
                     /locus_tag="J8I47_001298"
     CDS             complement(4362..5045)
                     /locus_tag="J8I47_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAZ6322744.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(5212..6099)
                     /locus_tag="J8I47_001299"
     CDS             complement(5212..6099)
                     /locus_tag="J8I47_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6322745.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(6096..6965)
                     /locus_tag="J8I47_001300"
     CDS             complement(6096..6965)
                     /locus_tag="J8I47_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6322746.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(7102..8388)
                     /locus_tag="J8I47_001301"
     CDS             complement(7102..8388)
                     /locus_tag="J8I47_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6322747.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            8661..9239
                     /locus_tag="J8I47_001302"
     CDS             8661..9239
                     /locus_tag="J8I47_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ6322748.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSLDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            9239..10168
                     /locus_tag="J8I47_001303"
     CDS             9239..10168
                     /locus_tag="J8I47_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322749.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            10165..11901
                     /locus_tag="J8I47_001304"
     CDS             10165..11901
                     /locus_tag="J8I47_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6322750.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(11964..12800)
                     /gene="pheA"
                     /locus_tag="J8I47_001305"
     CDS             complement(11964..12800)
                     /gene="pheA"
                     /locus_tag="J8I47_001305"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAZ6322751.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(12797..13306)
                     /locus_tag="J8I47_001306"
     CDS             complement(12797..13306)
                     /locus_tag="J8I47_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAZ6322752.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(13299..14594)
                     /gene="aroA"
                     /locus_tag="J8I47_001307"
     CDS             complement(13299..14594)
                     /gene="aroA"
                     /locus_tag="J8I47_001307"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAZ6322753.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(14607..15698)
                     /locus_tag="J8I47_001308"
     CDS             complement(14607..15698)
                     /locus_tag="J8I47_001308"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAZ6322754.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(15715..16881)
                     /gene="aroC"
                     /locus_tag="J8I47_001309"
     CDS             complement(15715..16881)
                     /gene="aroC"
                     /locus_tag="J8I47_001309"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAZ6322755.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(16883..17947)
                     /gene="aroB"
                     /locus_tag="J8I47_001310"
     CDS             complement(16883..17947)
                     /gene="aroB"
                     /locus_tag="J8I47_001310"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAZ6322756.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(17980..19002)
                     /gene="aroF"
                     /locus_tag="J8I47_001311"
     CDS             complement(17980..19002)
                     /gene="aroF"
                     /locus_tag="J8I47_001311"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAZ6322757.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         5578 a         4261 c         3409 g         6235 t
ORIGIN      
        1 ctttaatcta tcgcaggtat gtttttactg tctattcttg tttttctgat tgaaaattca
       61 attttacccc aggctgatct aaggcgatct ctgtcacctc ataagacatt cggcgtacga
      121 tatcgaataa cggtgctact agctcgtcca cttcttcttg tgtgacatca ttttgacctt
      181 caattttccg attgaggata tgattgatct gactcacact tccagataaa acatatgtat
      241 caaacactaa acggaactct aagcgtgctc ctacgatgct atgttctttc ggatagtttt
      301 cgatcgtttc ttttaaaggc gagaagccta ctttcaaatc tgtttctact tcttggttcg
      361 gcaatctcat atcataatga aatgcttcta caacttcttt ttgtcttctg atttccatat
      421 aattttatca ctcctttatg aaaattataa catatctcct ttgaaagggg atactacttt
      481 ttaatgatgc tctgcaaggt aaggatttca ttcagatttc ctaccacata atctgccggg
      541 atctcaccca gaaaatgatc gatgtcgtaa agacaagtag cgactcctgc atttttccct
      601 gcttcgatat ctaaacgacg atcgccgatc atcatcgtgt ctgttctttc taaatgaaaa
      661 gtatcgatca aataatttaa agatgctgga tcaggtttac gaggaaagtc ctgatcgatc
      721 cctactactt cctcaatcag atgtgccaat cgatgttctt ttaacaggcc ccatgttgat
      781 tctgtcaaac ggtgggtcag gatgaaatga cgaccgccgt tgtcttttag catttccagt
      841 acttgtttcg tttctggaaa tggacgggaa acttccgtac tttctttttc atatcggtga
      901 aataattcat taaactctct ggcattcaag tttgattcat tgatcaaata actagtagaa
      961 tactttttca ttttgaagta gatttctttt ttgtccatac tgataccaaa atcttttagt
     1021 gcttgccatg caccatctac cattgcggga tacgtatcaa acaatgtacc atcaaagtcc
     1081 catatataat ttttcatgtt ccttttgttc cattcctttg cttctttttt ctatctctca
     1141 cttttagata aaggtgttct ttaaagtatt tttttgtcca gaattgtcaa atcatctgct
     1201 aatgtatact ggattggttg tgcatttggg atatcgatcg tatccatttg atcttcagga
     1261 acatcttcta aatattttgt cagtgctcgg atgctattac catgacccac aactagtaaa
     1321 tcttgccgat cttttagttt tggtgctaga tgatcttgcc ataagggaat gatccgattc
     1381 actgtcattt ccagactttc tccgtatggg atcaaatgcg gatccaagtg atcatatctg
     1441 cggtcaaatc tattttctgc ggttaacggc ggaacttccg tatatcctct gcgccatgtt
     1501 tttacttgat cttttccgta tatttctttc atcttatctt tgtttttacc cacaagatcc
     1561 ccataatgcc gttcatttag acgccatgtt tttattatag gaagccataa ctgacccatt
     1621 tcttctagtg cgacttgtcc ggtaagaatc gcacgtttca aaacagaggt ataaacggca
     1681 tcgaattgaa gtccggcttc aagcattttt tgaccggcaa ctgctgcttg ttcttttcct
     1741 ttttctgtta aggcgacatc tagccatcct gtccagtaat tttcaaaatt tgcttcgctt
     1801 tcgccatgac gtaataatac taatttcatt cttttctccc ttaaacattc gtttcgaatt
     1861 ttataccaca tgttttgcat gtcacacgta ttcgtccttt tcctttaggt gcacgctgct
     1921 tttgatgaca attcgggcac tcaaaaaaag tataactttt tcgatccatt ttgacttgct
     1981 ttttgttttt tatctgatga tatttttctt tgactctatc aagatatttc aaataacgtt
     2041 gattctcatt tagtctgggg tagatttttt ttgataggaa acgaaatatc atccatccga
     2101 aaaagacgat gaacaacaat tgtccgattc tatagggcaa ccaacgactt agcaaactaa
     2161 agacaagcgc agcgatcaat agaccgcgat tcaactgatc aaatttcgca tatcttccct
     2221 gcataagttt gtgatagttc tgctgaaatc gtattagcct ttctaaccat gtttgcatca
     2281 tattatctcc tccatcatct aatatttatt atgacaaaaa aatccaaaaa gaaaaagcct
     2341 taattggcct tttccattgc aacttggtga aggacattcc gatttctggt aaagtcaatc
     2401 acaatcactt tctttttatc ttctctgttg actactcgaa atgtgtccgt caatggaaga
     2461 aacagcttct tagatggtag ctgttgcata cgtataaaac gatggatcat cgtaatcaat
     2521 tgaggaaatt gaatagacgt gtgaagcaga cctggatatt ttttcaacag ctgtgtcgtt
     2581 ttgatcagat tccgtttttt tactgatcac ttttaaaaag ctttttagaa tcttttgctg
     2641 acgtctaaaa atagaaaaag aattttcgtc atctatttga taacgcattt gtttttgcag
     2701 ttctgctgca gtcagatgga tcttcccatc gtttgtatcg atcattaaac cttctggaaa
     2761 cagttcaacc actgctcgat cccaagattc aagagaaagc tcgatataat cttttactgg
     2821 taattccaat tctaccttaa aaaagtgcaa aacagccgac agcccttctg cttgatagat
     2881 ttctcctaat gttagcccct ctagatggaa ctctggtgaa aaatgcagca atgttttatt
     2941 ttcctgcaat tgaacatata gaaatgtaga tcgctcaatc agagtatctt ttaaaacaac
     3001 taaaaaattc atttttctcc cccttttttc atcattttta aaatagcata ttttgaaaag
     3061 aaagtctgat gcttagagta gaatttcata aaaatttact atttcataag tattcacgag
     3121 aaaaatacaa acaaaaaagc ggaaataacg ctcatccgcc ttcttgtttg tgtttatttc
     3181 ctcttcaaac gcttgtctat gattcagact tttctctatc gttattttca tctaaaagtt
     3241 cttgcattcc gataatttct cgatattgat gaatcttagt gataccaatg actttgtcta
     3301 catcaatact aaaacaaata ccattacctg gaatatcgat gttgatgcct tgaccaatcg
     3361 catccattac tttattcgtc aaactatcgg gtactaatgt caacacaata tctttttctg
     3421 gcgaaatgtc caaacctaaa aatttccctg aatcatgtac accagaacct cttccatgta
     3481 agatcgttcc gcctgctgca cctgcttctt ttgaaaagtc gataacatct tcgcccattc
     3541 ctccgtcaac gatcgtcaca atcatgtgaa gattcaatgc tacaagatct ttcatttgct
     3601 ttcccccaat ttttgcaatc tttctttttc gattctgtct ttttcatctt ttcgtttata
     3661 aatatatcct agtactaaag tggataatat tggtgtcaat gcaatcattg caaccacacc
     3721 aaacccgtca atgatcggat ctcttccttc aatttgtgca gcgattgcca cgctcattga
     3781 taaaataaat gtcgaacaca tcgggcccgt tgctactcca ccgttatcaa aagccattgc
     3841 taaaaagatc ttgtccactt ttcgtcctaa aataaatgct agcccgtatc ctgggatcaa
     3901 gaaataataa agagagaatc cgacgagcaa acgccacata gacaatccta ctgctgcgcc
     3961 gatcccaatt gaaattacca tcagcatgat tttcgatcgg attctcccct cgctcaattc
     4021 ttcaacctgt ttgatcatta catgaatcgc cggctcagca aaccctacta agaaccccat
     4081 taaaaagcca aggggaacca gtatataatt gtaaggcaat cccgcaatag cactgcctaa
     4141 atgctgtcct accggtacat aagccatatt gactccgtgc aggaaaagga tcaacccaat
     4201 agtcgttatc aagaaacctt tcataatgtc aaagaaacgc tttttgcgca tacgaaaact
     4261 aatgatattc attaaaacaa ataggattac gataggtgcc actgccaaaa tgacttccat
     4321 ggtaacgatt tcgattccat ctatcacttg ctgccagaaa ctcattggaa gatcaccccc
     4381 aaaatcatca cggcaagaat cggccctaac gaagcaatac cgaccattcc aaaactgtca
     4441 ttttcatttt tcttttgttt gatcatacta gtcatccctc cggcaagggc caagataaat
     4501 ggaacagtta ctggaccagt agtcactccc cccgaatcaa aagcaatcgg cataaattcg
     4561 gaactggtga aaaatgaggc gatcaaaacg cctatgtagc ctatagccat caattgataa
     4621 taggatagct tgaatacgac ccgaagcagc gcaaaggcta gaaatactcc agtgccgacg
     4681 ctgacgatcc caatcagcaa cactctgcca atctctccgt cagatatatc atatacttgt
     4741 tgtcccaaaa cttgcacgga aggttcagca atcgtgatca cgatcccgat agcaaatccc
     4801 aaactgacca aaacggtcaa acttttcttt ttgatcatat atttccctac tagatctccg
     4861 acttccatca tagaataatc tgctccaaac aaaaagagcg tcattccgat catcatgatt
     4921 gccgcaccaa ccagaaatga agcgaggtct tctccttcta acggtgcaaa aacaaacgtt
     4981 aaaatgacaa tcaggacagt catcggcaat atagcagcaa tcacttcttt aaaatttttt
     5041 agcatgtatc ctcctccttt actctttaag tttaccttaa aatgaaagag ttttcgagca
     5101 caaacttaaa acgttatcat ttaatttttc tcaacataaa ataacacacg tcccaaaagt
     5161 atctttttac ttggaaaaat gactttgaag gaacgcgctt attgtgtttt tttattttac
     5221 agagccgatc attccttgaa cgaattgctt ttgcaaaaca aaaaatacga tggcagttgg
     5281 taaagtagcg atgactaatc cagccatgat cattccatag tctggtgtat aggaagcacc
     5341 catagcagat aaaacaagcg gaaccgtccg tttatcagga gattgcaaag ctactaaggg
     5401 ccataaataa ttgttccagc tactcatgaa tgtgatgatt gcagccgcgg cataggtatt
     5461 tttagccgtc ggcatataca ctctaaaaaa aatacttagt tcttttagac cgtctaatcg
     5521 agcggcttct actagatctt ttggaaaaga tttaacattt tgtcgaaaga agaagatcaa
     5581 aaaagcagtg ctaacagatg gcaggatgac aaccaagaaa ctgttgattc ccaaaaccgt
     5641 tccgttcaat tgtgaaaaca ttcgatataa agggatcatc aatgccgcaa aaggaaccat
     5701 catcgatagc agtagaatat tgaaaattag gtcttttcgt ttactttgat aaatctcaaa
     5761 tccatatcct gccattgacg aaatcagcaa tgcaaacaca gtggtcacca aagcgatcat
     5821 cgctgaattc caaagggaac gagtaaaatt cagctcattg gaaaacaaat tttgaaaatt
     5881 gatgatcaag ttactgccaa tctttatttt tcccgatgtg atatccacgg gtgtattcgt
     5941 cattcccaaa accatccaga caaaagggaa aatggaaata aatgatagga tgcttaatgc
     6001 tgtatacttt aatactgacc caattgtcga acgaagttct aatgcttgtt ttttgacgtt
     6061 ggggcgttcg attgtttctt tttctaccat atttttcatc gactttctcc tcctactttc
     6121 gtttggatta tcgacaatat cacaataaag gaaacaataa caaaggaaac tgctgccgca
     6181 tatccaaaat taggcgtgaa tttataactc aaattataga tgtattgaga tagggtggtc
     6241 gttgcatttg ctggtccccc gccagtaata ttttgtactt catcaaaaag ctgcaatgtc
     6301 ccgatcgtcg aagtaatgga agtaaacaaa atgattggtt ttaaatttgg gatcgtcact
     6361 ttgataaact gctgccattt agaagcaccg tcaatagatg ctgcttcata aatctccgaa
     6421 tcaatatttt gcatgcctga aaggaaaaaa atcatattgt atccagtcca acgccaagtg
     6481 atcgaaagaa tgatcagaac tctcgcccaa attggatgag tcaaccagcc aatcggtgtg
     6541 tcaatcatac caatgcccaa caacaagttg tttacaaggc cagattgaga aaatagactt
     6601 ttcatgatca gagaataact gaccatcgaa gtgatacatg gaagaaagat agcggttcga
     6661 aacaaccctt tatatttcaa ctttttatca tttaaaaggt tggaaataaa aagtgctaga
     6721 aacaacatga tcggtacttg tacaagtaaa tacaggaatg tattaaaaaa agctttttta
     6781 aaagttgcat ctgtcaacat ccgctgataa ttacctaacc cgttaaatgt catattgaca
     6841 gggggaccac tttgaaaaga cgtgatcaaa gccaaaacca tcggaataaa aaccatgatt
     6901 gatatcaaaa tgactggcaa gattaaaaaa gcatgcccgt ttttgaataa atctttctgc
     6961 ttcattcttt tccctccaat tctatcattg attatctaca tgaaaagaca agaaagacaa
     7021 tatctgtaag gcagacgtga tcttttttgt cttttcgttt gtctgatgaa aaagcgaaga
     7081 aattcacttt tttaccaccg atcaattagc caattgtgct tctacttgtt tttgtgtatc
     7141 tgctagtacc ttgtctgtct cttctccatt gatgattcga tgaagagctt cagctacgac
     7201 tgcttcaatt gcataggtct catgtccgta attcacttct ggtatttcag atgtccatgt
     7261 tgaaaaatcg tcaaagatct tttgaccact gtaaaattca gaagcttctt gataagcttc
     7321 ttgttcattc gcgctaagca tcgtagatac tagtccgatt tctttcgcaa gagtgcccat
     7381 cagttcttca ttcgaagcaa acgttgcttc taggaaatct ttcgctgaat cttctccagc
     7441 tactcctttg atcacatacc agccagcgcc gccaaggtta gaagcttgcg ctttttgcag
     7501 attttctgga agtgcaggta ttggtgcaat cttccatttt ccagattggt tttctgctcc
     7561 ttggatcgtt gaagaatacc aagcgccagt tgggctagaa gctactgctc ctgattgtat
     7621 cgcagttact ccagcattcc agtcagaggt ttgttcgact agatcttctt tcaaaagagt
     7681 ggcaaacaat tcaagaccat actttaaaga tgcattgtct tggatcgtga ctgtctcccc
     7741 atcttcagat gtataccatt ctcctgcctg ttgcataatc atccttactc gaccaaggtc
     7801 agaaggattg acttcagcga ttttctttcc cgtcttttct ttgacatctc gcgctacttg
     7861 aatatactcg tcccacttca gattctccat atcctcttct gtataacctg cttctgccat
     7921 cagatccgta cgatagaagt ttgctgttac gcccgaatcg aaaggaacac cgtatatttt
     7981 atctcctact ttgtttaccg caaatttata cggtgcaaaa tgatcttcct ttacaatagc
     8041 agacaaatta gcgaatgcat ctggataaga agtcaaatag ccttgtatcc tataatcttc
     8101 aatcaagacg atattgggca atccttctgt attcccgctc gctaaagcgg tgttcagttt
     8161 ctgaacgata tcatcctgag acatcgtaac aacttctatc tctgtttctt cgttctcata
     8221 aacttttttt gcttcattca ctgctttgat attgaatgtc tcatcccacg cccagacagt
     8281 gactttttct tctttgtctg ctgtactact agcacttccg catcctgtta agaaacctga
     8341 tgctgccgct aaacaaacga ctccagcaat taactttctg atgttcattt ttatccgctc
     8401 cttttccatc cattagttgt ttacgtttgc tacattcatc ataccaaaat ttgaaaacgg
     8461 tttctttatt attcagatac ttcaaaaatt tctcctaaaa taataaaaaa atacaaatca
     8521 actatgacaa aaggaataat cttagcatct agaccaaaga tcgaaatggt gaggtttctt
     8581 taaaataaaa atatccttgc attttctcct agaaataagt atttttaaat tacaagacta
     8641 ttcttaaggc gggtaaaaaa atgccaatac gcgtaattct tatcgatgat gaacagacga
     8701 ttttagatgg aatgaaacag ttgatcaatt ggtctgagca cgggtttcaa ttaataggga
     8761 ctttttccag tccgctgaaa gcgcttgatt tttctttggt acatgcaatc gatatcgtgg
     8821 tcacagatgt aatgatgcct gaattaaatg gaatcgaatt atcgcggcta ttaaaacagc
     8881 aacaacctga aacacagatc cttatcctct ctagctatga tgaatttgat atggttaaag
     8941 attcttttaa agaaggcgtc gctgattatc ttttaaagcc tcgattgagt cttgattttt
     9001 ttctaaattc cttaactgcc gtttcacaaa aaatcaaaaa aaagcccaac aaaagcccaa
     9061 tcaccaatcg ttatgagcag atctgcgaga atctttcaca gcagattgct ggacaaactc
     9121 gtccattgct tgaccctctt ctttttgaac acaccttgtt tttctgttat ataccgaaaa
     9181 acggccattc agttcaaaac aaggctctaa acaaaaacag tcgatcaccc attcattgat
     9241 ggatagcgac gattcgttta ctatttatcc tgtttctacc ttttcggaag aatccggcta
     9301 tgtaatcaat actgattcca aagatacact taggaagttt gttcaaaagt ttcgtcaaga
     9361 agtttttcca ggtgattttt ttgttgtttc tgattctttt gcaatccaag atatcaatga
     9421 aatattccaa gagatcagaa attgcaccaa aggacaacgt ttctatcata agggaaagaa
     9481 ccttgcttat cagcatgaac ttcttccgtt attaaccaac cacgagcagc aagcaagtag
     9541 ttatttaaca aaaatcgttc ataaagacta cgctttagct gtcaaagaac ttgagaattt
     9601 cttcacgacg atattggaac agcctattga tccagcttat ttgaaacatg aggcaatcaa
     9661 tcgtttctat gcattactca acgctttggc tgacgagcat gttgggaatg aagaactttc
     9721 tttgctcaag atcgcccttc cttctttatt agccgatgct gagtatttcg aagatttttc
     9781 tattcttgtc aaagaaacac ttgagaaact cacccacttt tctgcaaatg caaaaaaaga
     9841 caatttcgat atcttgactt tgatctacga gttcgtccag gaaaattatg agaaagaaat
     9901 caatttacaa cttttatccg aaaaatatca tttttcatat agctatctct cagccatctt
     9961 tactgaaaaa tttggtatca acttttcaaa atatctaaaa aaagtccgta tcagtaaagc
    10021 caagctcttt ttgacggaaa cacggcttac tttgacggaa atctgtgaga aagtcggcta
    10081 taccgaactg ggttatttct ccagagtttt taaagaagag acaggaatga caccttctca
    10141 ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca aagagcatga actctttgcc
    10201 aaacttctgt tcattttgtt catcggtctt tttctgcaag ccatcgtcat ttctttgttt
    10261 atttatcatc gatcaaggga tgcctatatc cagctgttca atcaatcgaa tgatgtggta
    10321 ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata caatcgagaa tacattagcc
    10381 gcttttgatt ctaatccagc tgtcaaaagc tatttttcca atgatccagc ccaacatatg
    10441 gaacagcttc agcaactaag aacaatccaa aaaatgaatg actctctttc aaaaatccat
    10501 ccaatgatcg attacgatgt attgatcttt ggagaaaatg gaagaacttt tgtcggaaac
    10561 gacatgctca ctgccgtatc tgccgattct ttttttcaat ctgcgattgc tcagagagtg
    10621 aatgaacgtg ctgctgatac acaaatgctt tttgcacacc acggattgac tcttcgcgat
    10681 aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa atacattgaa tcacgtttac
    10741 ggatatgctg ttttatcgat ctcatccaag caactagcga atctttttca atctgtagta
    10801 aatcctgaga tatcgaagat ctccttcatg aacgaagaac agacgatcat cgcatcaaat
    10861 gaagaaaata caattgggag ccgatcaatg attttcaaaa aactaacagt aggtgaaaca
    10921 acgatttcaa atggacagca gttgacccgt cttcctctgt accgccagga ttgtacatta
    10981 ctcagtcaag tcgatcttca ttctttatct aaccaaatgg gcgtgatttt tccgattatt
    11041 ttgtataata tcgtgacact tgcttttgct ggctcattgg tatttatgta tttggatcga
    11101 cacacaaaat cgatctaccg cttgattgat tcgctaaaac ggctagagga agatggctta
    11161 aaagctactg ttcctgtaca aggaagccat gaagtccaat tgctagggaa tacgatcaat
    11221 caattgctaa gtaatgctca gcatcattat gaacagacga tccaagacga aaagaagaaa
    11281 cgttcgcttg aaatccaaac tatgcaagca cagatacagc cccattttat ttataatacg
    11341 ctgactagct tgaaattttt gatttggcag aaaaaaacag atgaagcagt tcaaggaatc
    11401 gacagtttca ttactttact aagaagtacg attgggaaaa aagaagaagt gattccagtc
    11461 aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt tgagtatgcg atatggcgaa
    11521 agaatctcgg ctaaaataat gattcctgat gaactgcagt cattatctat gccgaatatg
    11581 atccttcaac caattatcga aaacgcctat cttcatgctt ttcacaataa aacgcaagga
    11641 ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt tattcgaagt gattgataac
    11701 ggcgatggct tcgatactga aaataccaaa caaaaaaatg atcatttttc aggaatcggc
    11761 atcagccatg tagatgagcg gatacgccta atgtatggcg atgcatatgg cttaacgatc
    11821 caatcagtga ttgggacagg aacgactgta acgatttgtc taccgcttct tcctaaaaat
    11881 caatcagaat atcaaaaata aagaatagcc aagcgctgat ttttttattt taagttaaat
    11941 aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag cactggataa
    12001 cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc attctctact
    12061 aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc caagctagtt
    12121 ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa agctgccagc
    12181 attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact catttttact
    12241 ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg tgtctggttc
    12301 aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg tgctgtttct
    12361 aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat tgctgcagca
    12421 gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa aaactgttgt
    12481 gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc tggattgcct
    12541 aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc tggatgtttg
    12601 gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac aggaacgaca
    12661 gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga agcatacgca
    12721 gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga actttctggc
    12781 cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg atgatctttt
    12841 ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac caagaaagtc
    12901 gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa ggtcgaatag
    12961 acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct aagtaaagaa
    13021 caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc cctccaccag
    13081 tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc acttttcgaa
    13141 agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata tgttcctcaa
    13201 tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct ttaccgattg
    13261 ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc ttctcctcct
    13321 gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac agcttcagct
    13381 ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat cataccgatt
    13441 cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc atggatgatc
    13501 agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc tgccaccgca
    13561 tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat cgtgattcct
    13621 tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg cggaatgatt
    13681 tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc agccgattgg
    13741 ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa cacatctaag
    13801 ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct ttccggaaca
    13861 agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac agttatctgt
    13921 tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac tgtgatcctt
    13981 ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt ttcaacgatt
    14041 acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac ttgcgcactg
    14101 gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac agtgattggt
    14161 agatactggt cattttcttc tccttgcaag tcagcgccca tctcgcgtaa aggttccatc
    14221 actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt cgtaaaaggc
    14281 tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc catgtcaatt
    14341 gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat cacctcttcc
    14401 tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag acaatcttct
    14461 gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa catgatgctg
    14521 cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc atgtatttgt
    14581 tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact ggtattagac
    14641 ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat ttgcagtatt
    14701 cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac tcccgctttt
    14761 ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc gataaataaa
    14821 tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg actttctttt
    14881 gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca ttctgctatt
    14941 tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt attgctcaac
    15001 aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc tcgaaagcct
    15061 cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc ctgattgact
    15121 aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg gtcatgttct
    15181 cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg cagtttttca
    15241 acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc aaatagttta
    15301 gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc accgacaaac
    15361 cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct gacatccgta
    15421 atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc taaggttgta
    15481 cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc aaaatcactt
    15541 gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga ctgactatcc
    15601 caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat agaagcacca
    15661 atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc ctttttagta
    15721 atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa gcgaatcact
    15781 tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt ccgcaacgac
    15841 gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt aaggttcttt
    15901 tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt tcatgacccc
    15961 gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta gattattggt
    16021 acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt ctgagcctgg
    16081 taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat tgatactaac
    16141 cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc ttcctaatcc
    16201 agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc catttttctt
    16261 tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa ctcgtacttc
    16321 tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta tttctgcttg
    16381 gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt ccagaatttt
    16441 cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg accgttcaag
    16501 cacattccga agatctcgat gatggtattt cataccgcct accaaatccg cgtgtcctgg
    16561 acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg ctacagacat
    16621 gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag gtgaaccaat
    16681 tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga tcttcatcct
    16741 tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt cttcttctga
    16801 cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc catgggattc
    16861 tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat gtctctgtgg
    16921 gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc aattggcttc
    16981 ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga cttagatcgt
    17041 acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc gatccagttg
    17101 gagacaagcc gattttttct gcatgagaag tgatggctac cattccaata gctactcctt
    17161 ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca attgtatgac
    17221 caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact acttttcgtt
    17281 tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct ttttcgtctt
    17341 tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat ttgatgattt
    17401 cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca ggatcaatca
    17461 atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt gtattgactg
    17521 ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga atttgaacga
    17581 aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc accattcctc
    17641 caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa aaatcgtata
    17701 actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt gaaaggactg
    17761 gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt ggagcaacgt
    17821 ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc catgatccag
    17881 cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca acatgtacag
    17941 taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg tctgaatctt
    18001 ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag tcttttcatt
    18061 caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct ctacgatcat
    18121 gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt cccaaattcc
    18181 tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca tcaatgggac
    18241 agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc cacgttcgat
    18301 aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa gccattcatt
    18361 gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac cgacagcaga
    18421 tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc tgtactcagc
    18481 caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc caaattcatc
    18541 agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag catatggaga
    18601 agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt tggccattcg
    18661 cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca tcgtcacgaa
    18721 actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag gatggaattc
    18781 tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc cttcatagcg
    18841 tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca ctactcgttc
    18901 tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga cgactttttt
    18961 tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc ccgctttctt
    19021 ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt ttacattaat
    19081 tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc tacttatgac
    19141 ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt gaacatcttc
    19201 aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga atttctctac
    19261 gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct ggtttgtttg
    19321 tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac gtattcagtt
    19381 ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa ttgtacctag
    19441 tatagacata aaaaaatcga ctgtcaatca tttttatgaa act
//
LOCUS       DADOZM010000043        19456 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.044, whole genome shotgun sequence.
ACCESSION   DADOZM010000043 DADOZM010000000
VERSION     DADOZM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.044"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            132..3659
                     /locus_tag="J8I47_001312"
     CDS             132..3659
                     /locus_tag="J8I47_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ6322758.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="J8I47_001313"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="J8I47_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAZ6322759.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="J8I47_001314"
     CDS             7447..7788
                     /locus_tag="J8I47_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322760.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="J8I47_001315"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="J8I47_001315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAZ6322761.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="J8I47_001316"
     CDS             9145..11565
                     /locus_tag="J8I47_001316"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAZ6322762.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="J8I47_001317"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="J8I47_001317"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAZ6322763.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="J8I47_001318"
     CDS             12999..13736
                     /locus_tag="J8I47_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322764.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="J8I47_001319"
     CDS             13750..14565
                     /locus_tag="J8I47_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAZ6322765.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="J8I47_001320"
     CDS             14578..15225
                     /locus_tag="J8I47_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ6322766.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="J8I47_001321"
     CDS             15241..15897
                     /locus_tag="J8I47_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ6322767.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="J8I47_001322"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="J8I47_001322"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAZ6322768.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="J8I47_001323"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="J8I47_001323"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAZ6322769.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="J8I47_001324"
     CDS             18239..18757
                     /locus_tag="J8I47_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAZ6322770.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="J8I47_001325"
     CDS             18864..19364
                     /locus_tag="J8I47_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ6322771.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5396 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagc
//
LOCUS       DADOZM010000044        19130 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.045, whole genome shotgun sequence.
ACCESSION   DADOZM010000044 DADOZM010000000
VERSION     DADOZM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.045"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..703)
                     /locus_tag="J8I47_001326"
     CDS             complement(<1..703)
                     /locus_tag="J8I47_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322772.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="J8I47_001327"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="J8I47_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="J8I47_001328"
     CDS             1758..2837
                     /locus_tag="J8I47_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ6322773.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="J8I47_001329"
     CDS             2869..3462
                     /locus_tag="J8I47_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322774.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="J8I47_001330"
     CDS             complement(3572..3799)
                     /locus_tag="J8I47_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6322775.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="J8I47_001331"
     CDS             complement(3902..6490)
                     /locus_tag="J8I47_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAZ6322776.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="J8I47_001332"
     CDS             complement(6510..7169)
                     /locus_tag="J8I47_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ6322777.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="J8I47_001333"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="J8I47_001333"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAZ6322778.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="J8I47_001334"
     CDS             complement(7942..8808)
                     /locus_tag="J8I47_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ6322779.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="J8I47_001335"
     CDS             8917..9558
                     /locus_tag="J8I47_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAZ6322780.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="J8I47_001336"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="J8I47_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAZ6322781.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="J8I47_001337"
     CDS             complement(10999..11898)
                     /locus_tag="J8I47_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ6322782.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="J8I47_001338"
     CDS             complement(11900..12697)
                     /locus_tag="J8I47_001338"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAZ6322783.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="J8I47_001339"
     CDS             complement(12675..13379)
                     /locus_tag="J8I47_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAZ6322784.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="J8I47_001340"
     CDS             13510..14085
                     /locus_tag="J8I47_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAZ6322785.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="J8I47_001341"
     CDS             14203..14880
                     /locus_tag="J8I47_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAZ6322786.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="J8I47_001342"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="J8I47_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAZ6322787.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="J8I47_001343"
     CDS             complement(16943..17941)
                     /locus_tag="J8I47_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAZ6322788.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="J8I47_001344"
     CDS             complement(18167..18820)
                     /locus_tag="J8I47_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAZ6322789.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DADOZM010000045        18875 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.046, whole genome shotgun sequence.
ACCESSION   DADOZM010000045 DADOZM010000000
VERSION     DADOZM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.046"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(146..1111)
                     /locus_tag="J8I47_001345"
     CDS             complement(146..1111)
                     /locus_tag="J8I47_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAZ6322790.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="J8I47_001346"
     CDS             1345..2190
                     /locus_tag="J8I47_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6322791.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="J8I47_001347"
     CDS             2381..3568
                     /locus_tag="J8I47_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ6322792.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="J8I47_001348"
     CDS             3574..3930
                     /locus_tag="J8I47_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAZ6322793.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="J8I47_001349"
     CDS             3932..4270
                     /locus_tag="J8I47_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAZ6322794.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="J8I47_001350"
     CDS             4665..5699
                     /locus_tag="J8I47_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322795.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="J8I47_001351"
     CDS             5692..6378
                     /locus_tag="J8I47_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322796.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="J8I47_001352"
     CDS             6402..7250
                     /locus_tag="J8I47_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6322797.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="J8I47_001353"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="J8I47_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAZ6322798.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="J8I47_001354"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="J8I47_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAZ6322799.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="J8I47_001355"
     CDS             9516..10751
                     /locus_tag="J8I47_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ6322800.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="J8I47_001356"
     CDS             10738..11208
                     /locus_tag="J8I47_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAZ6322801.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="J8I47_001357"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="J8I47_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAZ6322802.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="J8I47_001358"
     CDS             complement(12699..13841)
                     /locus_tag="J8I47_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6322803.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="J8I47_001359"
     CDS             complement(13880..14614)
                     /locus_tag="J8I47_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322804.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14846..15121
                     /locus_tag="J8I47_001360"
     CDS             14846..15121
                     /locus_tag="J8I47_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6322805.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15219..17216
                     /locus_tag="J8I47_001361"
     CDS             15219..17216
                     /locus_tag="J8I47_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322806.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17292..17627)
                     /locus_tag="J8I47_001362"
     CDS             complement(17292..17627)
                     /locus_tag="J8I47_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322807.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            17754..17969
                     /locus_tag="J8I47_001363"
     CDS             17754..17969
                     /locus_tag="J8I47_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322808.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            18006..18368
                     /locus_tag="J8I47_001364"
     CDS             18006..18368
                     /locus_tag="J8I47_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAZ6322809.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            18414..18740
                     /locus_tag="J8I47_001365"
     CDS             18414..18740
                     /locus_tag="J8I47_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322810.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6256 a         3266 c         3943 g         5410 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcaaaa aaggggtgtt gcaaataaat tttacaaagc tgttgacacc ccttttttat
    14701 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14761 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14821 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14881 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14941 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    15001 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15061 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15121 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15181 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15241 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15301 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15361 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15421 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15481 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc gtagaaaagg
    15541 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15601 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15661 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15721 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15781 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15841 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15901 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15961 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    16021 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16081 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16141 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16201 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16261 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16321 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16381 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16441 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16501 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16561 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
    16621 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
    16681 aagatatcct tccacttctg gaaaaaattc attttacata tcaatatttt tcttatgaat
    16741 gggttaagat atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat
    16801 cagaactcag caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct
    16861 tatcgtggca agggacatat ctaatgacag agaaagaact tattaacact gtttgtgaac
    16921 gcctacgtaa tgttggggat aacagcgtga aaaatgcttt aataaaaaac agaggctact
    16981 ttgttaaaca aggattcact cttgaacgag cttctaagga tattaagaaa ctatacaatg
    17041 tgaaagcaag tagtagtttt ttaatatatt tagcgaaagg ggcagggata gatgaaacaa
    17101 taaaagaatt gatattatca ggagctggga tagttcctct gtatcaattt acaagtgtaa
    17161 caagagatga tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag
    17221 gttatccata gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg
    17281 ttatttattt ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc
    17341 ttcatatagt caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa
    17401 aactcttcct cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg
    17461 gctctagaaa gagctgttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt
    17521 gctactgcct gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga
    17581 gcttttcgct tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca
    17641 tctcagtata gtacaacaag ttgttgtatt tgtaatggta tttgaagttt tttatatttt
    17701 atgagttgaa atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa
    17761 aagtaagtaa tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa
    17821 aaaaaatgaa tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta
    17881 aaccctatca aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt
    17941 ctgatgtatt taataaagta aacagttaac taataagcaa ttgaatgatt ttaggaggag
    18001 aagacatgta ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt
    18061 tttgtggagc actacaagaa acagatgaga caaccggaac agacacagta aagaaaagta
    18121 ctgagcagac aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc
    18181 tgtatttgat tattggttgg atttctatgg tagtatcgtt agcatttatt cctgttgtat
    18241 ttggtgctgt tgctgttatt atgggctact tatatcgaga aaaagatgag aaaatgggta
    18301 cgattttgat gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa
    18361 tgtactgata agagggataa gagcaaatta ttttggagat aggagataaa aagttgaata
    18421 aagtaaatat gaacaagaag tttgtcatta taggtattgt tgtcttggct attataggta
    18481 ttattttttc aaaaatgaca ggtacatctg gattagagaa gtcatcaaaa ccattggtaa
    18541 cacagatttt acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc
    18601 atgaaaatgg agataacaca taccgggcta aagctatatt agacaatgga agtgctatca
    18661 acataaatat tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg
    18721 aggtgttaat gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca
    18781 aaggtgtaaa ttcactaggg agtttttata gtttcctata aattaaatac taatacaggt
    18841 gacaaaaaaa tattacaatg attgtcggga atttt
//
LOCUS       DADOZM010000046        18740 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.047, whole genome shotgun sequence.
ACCESSION   DADOZM010000046 DADOZM010000000
VERSION     DADOZM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.047"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            256..531
                     /locus_tag="J8I47_001366"
     CDS             256..531
                     /locus_tag="J8I47_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322811.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="J8I47_001367"
     CDS             544..2616
                     /locus_tag="J8I47_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAZ6322812.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="J8I47_001368"
     CDS             2781..4667
                     /locus_tag="J8I47_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ6322813.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="J8I47_001369"
     CDS             4679..5626
                     /locus_tag="J8I47_001369"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAZ6322814.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="J8I47_001370"
     CDS             5646..6173
                     /locus_tag="J8I47_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ6322815.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="J8I47_001371"
     CDS             complement(6236..6889)
                     /locus_tag="J8I47_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAZ6322816.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="J8I47_001372"
     CDS             7021..7863
                     /locus_tag="J8I47_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ6322817.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="J8I47_001373"
     CDS             8021..8917
                     /locus_tag="J8I47_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ6322818.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="J8I47_001374"
     CDS             8920..9633
                     /locus_tag="J8I47_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAZ6322819.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="J8I47_001375"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="J8I47_001375"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ6322820.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="J8I47_001376"
     CDS             10168..10395
                     /locus_tag="J8I47_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAZ6322821.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="J8I47_001377"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="J8I47_001377"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ6322822.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="J8I47_001378"
     CDS             complement(11181..11723)
                     /locus_tag="J8I47_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAZ6322823.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="J8I47_001379"
     CDS             complement(11960..12358)
                     /locus_tag="J8I47_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAZ6322824.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="J8I47_001380"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="J8I47_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAZ6322825.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="J8I47_001381"
     CDS             13471..14238
                     /locus_tag="J8I47_001381"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAZ6322826.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="J8I47_001382"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="J8I47_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAZ6322827.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="J8I47_001383"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="J8I47_001383"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAZ6322828.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="J8I47_001384"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="J8I47_001384"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAZ6322829.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DADOZM010000047        18244 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.048, whole genome shotgun sequence.
ACCESSION   DADOZM010000047 DADOZM010000000
VERSION     DADOZM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.048"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..267
                     /locus_tag="J8I47_001385"
     CDS             <1..267
                     /locus_tag="J8I47_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6322830.1"
                     /translation="ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLG
                     CQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            299..1021
                     /locus_tag="J8I47_001386"
     CDS             299..1021
                     /locus_tag="J8I47_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAZ6322831.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            1079..3118
                     /locus_tag="J8I47_001387"
     CDS             1079..3118
                     /locus_tag="J8I47_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAZ6322832.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3227..4378)
                     /locus_tag="J8I47_001388"
     CDS             complement(3227..4378)
                     /locus_tag="J8I47_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAZ6322833.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4532..5062)
                     /locus_tag="J8I47_001389"
     CDS             complement(4532..5062)
                     /locus_tag="J8I47_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322834.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5161..5511)
                     /locus_tag="J8I47_001390"
     CDS             complement(5161..5511)
                     /locus_tag="J8I47_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAZ6322835.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5668..7197)
                     /gene="ahpF"
                     /locus_tag="J8I47_001391"
     CDS             complement(5668..7197)
                     /gene="ahpF"
                     /locus_tag="J8I47_001391"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAZ6322836.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7207..7770)
                     /gene="ahpC"
                     /locus_tag="J8I47_001392"
     CDS             complement(7207..7770)
                     /gene="ahpC"
                     /locus_tag="J8I47_001392"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAZ6322837.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7914..8576)
                     /locus_tag="J8I47_001393"
     CDS             complement(7914..8576)
                     /locus_tag="J8I47_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6322838.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8760..10406
                     /locus_tag="J8I47_001394"
     CDS             8760..10406
                     /locus_tag="J8I47_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAZ6322839.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10397..11479
                     /gene="uxuA"
                     /locus_tag="J8I47_001395"
     CDS             10397..11479
                     /gene="uxuA"
                     /locus_tag="J8I47_001395"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ6322840.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11587..13140)
                     /locus_tag="J8I47_001396"
     CDS             complement(11587..13140)
                     /locus_tag="J8I47_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6322841.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13288..14456
                     /locus_tag="J8I47_001397"
                     /pseudo
     CDS             13288..14456
                     /locus_tag="J8I47_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14582..15661)
                     /locus_tag="J8I47_001398"
     CDS             complement(14582..15661)
                     /locus_tag="J8I47_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ6322842.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15764..16204)
                     /locus_tag="J8I47_001399"
     CDS             complement(15764..16204)
                     /locus_tag="J8I47_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6322843.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16261..16671)
                     /locus_tag="J8I47_001400"
     CDS             complement(16261..16671)
                     /locus_tag="J8I47_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ6322844.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16851..17285)
                     /locus_tag="J8I47_001401"
     CDS             complement(16851..17285)
                     /locus_tag="J8I47_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ6322845.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17385..18233)
                     /locus_tag="J8I47_001402"
     CDS             complement(17385..18233)
                     /locus_tag="J8I47_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6322846.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         6265 a         3386 c         3295 g         5298 t
ORIGIN      
        1 gcccttaagg gcattactat atccgttccg ccacgtaaaa acatggataa atcagaaaaa
       61 ctagaccaca gcttacttgg aaaacaaaga aaaacagttg agactgtttt ttcttcctta
      121 gaaaaattag gttgtcaaaa tttcaactca cgttctgtta aagggctaga gagtagattt
      181 gaaagcatat tactggctta cagtgttcta ttaagtcgag cacaacgacg ttttgaagga
      241 actttgagat attctttagg atattaaaat taactagcac cacgggttat tatctatttt
      301 gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg gattttcgtc
      361 tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta ccacactaaa
      421 tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct cggattcact
      481 tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta tcatgagaga
      541 ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt actcttatgg
      601 gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag ctccctatta
      661 tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc ctggtggagt
      721 caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca aaggagagca
      781 agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca aagaaggaga
      841 cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc agctgaaaga
      901 cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta gtatccaaac
      961 gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag acatatgtta
     1021 gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg aggaaaaaat
     1081 gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat
     1141 cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag aaacccaagc
     1201 agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca agggagatta
     1261 taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa
     1321 agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag gacttcagat
     1381 atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat ataaagcaaa
     1441 gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat tagacagaaa
     1501 tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa tggaaggaaa
     1561 tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag atcgaaatgg
     1621 tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa gtaaacttgg
     1681 agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg acttaacaga
     1741 agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact ttgtcccatt
     1801 gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc aagaagtaga
     1861 cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg tgggagatat
     1921 cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa tcggtcgctc
     1981 aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga aattaagcat
     2041 tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa
     2101 agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca gtctaggagg
     2161 aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag
     2221 ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag attacaaagc
     2281 ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt atgctcctgg
     2341 atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta tcgatccaaa
     2401 tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg gatcttttca
     2461 aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt tgatctattc
     2521 cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg
     2581 cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga atccagcaca
     2641 aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg gatatggaca
     2701 gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt ttaccaacaa
     2761 tggcacactt gtctatccta aattgattgc agataaagag acaaaagata agaagaatgt
     2821 catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg tgcaagatgt
     2881 aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga aaactggtac
     2941 agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct tgtttgcttt
     3001 caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag aagatgatga
     3061 ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt atcaataaaa
     3121 aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa
     3181 taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac acttcataaa
     3241 ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga
     3301 taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa
     3361 tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt
     3421 gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta
     3481 caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta
     3541 atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt
     3601 ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa
     3661 tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg
     3721 atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg
     3781 caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa
     3841 gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa
     3901 ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg
     3961 tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa
     4021 gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc
     4081 agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat
     4141 attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac
     4201 caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc
     4261 ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga
     4321 tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc
     4381 tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa
     4441 taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt
     4501 ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt cttattctga
     4561 tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata
     4621 gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa
     4681 tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca
     4741 agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg
     4801 caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac
     4861 tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca
     4921 aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca
     4981 caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat ttttgaatat
     5041 tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta acgtgaacta
     5101 gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc tctggaaaaa
     5161 tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga tcattttatc
     5221 taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta caccacaaac
     5281 aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc
     5341 gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac ccgtcagcca
     5401 acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt ttgctacata
     5461 cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca taatcagtca
     5521 cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag aagaaaaagc
     5581 gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc ttttttccgc
     5641 tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga atgctcctaa
     5701 agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac tatctgtaca
     5761 atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg tcactatttc
     5821 atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga gtaagccgac
     5881 tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc gatcgatata
     5941 ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat gtttgataac
     6001 agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt ttaattctgg
     6061 taagaattct agaacatata catgttttgc taacccagcc aaatcgattg ctgcttcaac
     6121 accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag ggccgtcaca
     6181 gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta cattgataga
     6241 acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata taccgttagc
     6301 taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt gattcatcat
     6361 aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt ttggaccttc
     6421 tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt gtccgccgaa
     6481 agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag ctgcactact
     6541 agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa acaactcttc
     6601 ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc gtccgctact
     6661 aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat catcgatttc
     6721 tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta atacagctaa
     6781 tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa cataactgtc
     6841 aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac tgattttcgg
     6901 tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat gaccgagggg
     6961 gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa aactaggtgt
     7021 gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta tttccttcgt
     7081 aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa aaacaatagg
     7141 agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt ctaacatatc
     7201 caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc cactttcttt
     7261 ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag cgcgaatctt
     7321 atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca tagcttgaac
     7381 gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct cttcatccaa
     7441 aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt attcgatttt
     7501 accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat cggtagagca
     7561 agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta agtcttctaa
     7621 ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac tccagttccc
     7681 cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat gtgcattaaa
     7741 ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa ttatttattt
     7801 ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta tttaataaag
     7861 attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa atgctactgc
     7921 tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag
     7981 tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc cttgagtaaa
     8041 tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac tcgtaaccag
     8101 cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc ttttcttcct
     8161 gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca
     8221 tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt
     8281 gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc tcggacaaag
     8341 cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact ttgaggtatc
     8401 gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg agtccggccg
     8461 atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc taagtcagca
     8521 tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct tttcatcttt
     8581 attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa aaacaatctg
     8641 gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga catagcgatt
     8701 tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg aggggaaaaa
     8761 tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa gaagtagaaa
     8821 tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct gtctggatcc
     8881 atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg
     8941 atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa gaaataattg
     9001 aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca agcagtggag
     9061 agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat ccagattgtt
     9121 ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa ttagttactt
     9181 ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat ataggaccga
     9241 ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt attcttgctg
     9301 cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc tctacagata
     9361 atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca aaagaatggc
     9421 aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa gaaattgttg
     9481 cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa
     9541 tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa ggaaataata
     9601 ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat cattttccca
     9661 atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca acggtagata
     9721 aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct ttagctatta
     9781 tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca gatttatcgc
     9841 tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct ccaggtatca
     9901 ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat ccaaatattc
     9961 ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt cgttatggtg
    10021 agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt gtattcatac
    10081 cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa ggaaaaccat
    10141 tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc gatattcaac
    10201 tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg tcaaataaag
    10261 aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa gaaatgttca
    10321 tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac ttacaagaac
    10381 atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg aagaaaagat
    10441 taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca cattgatgga
    10501 tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag aagaaattga
    10561 gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg ccattaaaat
    10621 tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc gaaacttggc
    10681 agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact gggtgaaatc
    10741 tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat ctgatgatat
    10801 tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg aatttgaatt
    10861 gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag cctatgcatc
    10921 agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa tcatcccaac
    10981 atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat actcgatttt
    11041 tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc gcatagtaga
    11101 cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc cagaaaatga
    11161 tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg cacatgttcg
    11221 aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat ccgaatacgg
    11281 ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta acgaatacat
    11341 ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat acggcttata
    11401 tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa
    11461 caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata agaaggatgt
    11521 tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt atgtatctta
    11581 tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg cttcttcttt
    11641 taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt ctggtgtgat
    11701 gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca aagggcagtc
    11761 gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg attcatttga
    11821 aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac gcgtatagtt
    11881 cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta ctaggtcttc
    11941 aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt gattttgttc
    12001 tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca cgctatgttc
    12061 ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa gaaatccatt
    12121 ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt caggtataaa
    12181 caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa catgaggaac
    12241 gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt cgattttcgt
    12301 gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt
    12361 aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat actctgtgaa
    12421 ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata tttttaatag
    12481 aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt ttttaaaaaa
    12541 agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg tagcagtact
    12601 tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct cttttccaga
    12661 aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg ctaaaaatgc
    12721 attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt gctgacttaa
    12781 actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat aactgactga
    12841 ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa ttccttcaaa
    12901 attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat tgagacgctc
    12961 aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca tatggaaatg
    13021 atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa gaaaaatatc
    13081 taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt gagtttgcat
    13141 gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt ttttttcata
    13201 aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta tttcttgcat
    13261 acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat atcaacattt
    13321 gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga gatagaaaca
    13381 gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca ggatgaattt
    13441 tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac catcaaagca
    13501 aaaattgact attcaggaga cttagcacct atactacaat cagctgttac atttgagaat
    13561 gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca acacaagcaa
    13621 gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc gctttttact
    13681 gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta tagtcagcgg
    13741 cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga agaaccaaca
    13801 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
    13861 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
    13921 aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc agaagaaaaa
    13981 ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
    14041 ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa
    14101 ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata
    14161 aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt
    14221 ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca
    14281 gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac
    14341 agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat
    14401 taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt
    14461 tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa
    14521 aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat
    14581 tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat
    14641 ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt
    14701 cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca
    14761 acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat
    14821 aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa
    14881 tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca
    14941 gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga
    15001 caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt
    15061 aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg
    15121 tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg
    15181 ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt
    15241 caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt
    15301 ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa
    15361 gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca
    15421 tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa
    15481 tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga
    15541 aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa
    15601 aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca
    15661 ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat
    15721 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
    15781 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
    15841 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
    15901 cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
    15961 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
    16021 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
    16081 tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa
    16141 tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
    16201 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
    16261 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
    16321 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
    16381 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
    16441 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
    16501 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
    16561 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc
    16621 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
    16681 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
    16741 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
    16801 caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat
    16861 ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc
    16921 cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct
    16981 gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata
    17041 tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa
    17101 ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact
    17161 tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt
    17221 tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta
    17281 tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt attataccag
    17341 ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga
    17401 taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga
    17461 tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat
    17521 aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt
    17581 ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata
    17641 acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca
    17701 atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca
    17761 acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa
    17821 atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact
    17881 cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt
    17941 gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag tactttaaaa
    18001 aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa
    18061 ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag accgctgaca
    18121 cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag
    18181 agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttattca
    18241 cttc
//
LOCUS       DADOZM010000048        17393 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.049, whole genome shotgun sequence.
ACCESSION   DADOZM010000048 DADOZM010000000
VERSION     DADOZM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.049"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(176..1462)
                     /locus_tag="J8I47_001403"
     CDS             complement(176..1462)
                     /locus_tag="J8I47_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAZ6322847.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="J8I47_001404"
     CDS             complement(1455..2723)
                     /locus_tag="J8I47_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAZ6322848.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="J8I47_001405"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="J8I47_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAZ6322849.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3496..3942)
                     /locus_tag="J8I47_001406"
     CDS             complement(3496..3942)
                     /locus_tag="J8I47_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6322850.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4035..4280)
                     /locus_tag="J8I47_001407"
     CDS             complement(4035..4280)
                     /locus_tag="J8I47_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322851.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4396..4974)
                     /locus_tag="J8I47_001408"
     CDS             complement(4396..4974)
                     /locus_tag="J8I47_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ6322852.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5051..5581)
                     /locus_tag="J8I47_001409"
     CDS             complement(5051..5581)
                     /locus_tag="J8I47_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAZ6322853.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5585..6445)
                     /gene="rlmB"
                     /locus_tag="J8I47_001410"
     CDS             complement(5585..6445)
                     /gene="rlmB"
                     /locus_tag="J8I47_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAZ6322854.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6438..6833)
                     /locus_tag="J8I47_001411"
     CDS             complement(6438..6833)
                     /locus_tag="J8I47_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAZ6322855.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6830..8242)
                     /locus_tag="J8I47_001412"
     CDS             complement(6830..8242)
                     /locus_tag="J8I47_001412"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAZ6322856.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8239..8775)
                     /gene="cysE"
                     /locus_tag="J8I47_001413"
     CDS             complement(8239..8775)
                     /gene="cysE"
                     /locus_tag="J8I47_001413"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAZ6322857.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9097..10557)
                     /locus_tag="J8I47_001414"
     CDS             complement(9097..10557)
                     /locus_tag="J8I47_001414"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAZ6322858.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10727..11884)
                     /locus_tag="J8I47_001415"
     CDS             complement(10727..11884)
                     /locus_tag="J8I47_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAZ6322859.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11918..13288)
                     /gene="radA"
                     /locus_tag="J8I47_001416"
     CDS             complement(11918..13288)
                     /gene="radA"
                     /locus_tag="J8I47_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAZ6322860.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13351..13878)
                     /locus_tag="J8I47_001417"
     CDS             complement(13351..13878)
                     /locus_tag="J8I47_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAZ6322861.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14108..14914
                     /gene="proB"
                     /locus_tag="J8I47_001418"
     CDS             14108..14914
                     /gene="proB"
                     /locus_tag="J8I47_001418"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAZ6322862.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14911..16158
                     /locus_tag="J8I47_001419"
     CDS             14911..16158
                     /locus_tag="J8I47_001419"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAZ6322863.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16234..16578
                     /locus_tag="J8I47_001420"
     CDS             16234..16578
                     /locus_tag="J8I47_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322864.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKELVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16695..17021)
                     /locus_tag="J8I47_001421"
     CDS             complement(16695..17021)
                     /locus_tag="J8I47_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322865.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4741 a         3727 c         3174 g         5751 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaaagaaag ccgattttca gtgtcggctt
     3481 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
     3541 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
     3601 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
     3661 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
     3721 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
     3781 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
     3841 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
     3901 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
     3961 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
     4021 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
     4081 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
     4141 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
     4201 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
     4261 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
     4321 aattttcaag tttaccataa aaaaaaaaag aaatgcaata aaaataaata agaagctcat
     4381 taagattttt ctaaattatt cctcattata aaatttcaag tgttttttta ttttgagttt
     4441 tactctcccg taagcgctca ttacttgctt gtgatccatt gctaaacatt cagccatgtc
     4501 tacaaaatca acgccttgca tataataatg aaaaatttta gcttctattt ttgaaaactg
     4561 gatttcgcaa ttttcgattg cttcttttaa taaaagatgc tgaattgcta aaggttcttg
     4621 caatgtgatc tctgctaaaa agtctggtcc ttctgtctct attttttggt cgattgagac
     4681 ttcatctttt tgatcacgac gcttcagcgc gttctgcttc ctcaacagag aatagatttg
     4741 attgcttata acgcttttat aataacttcc aaagctaaca cctttgcttt tttcgtaagt
     4801 tctcaatgcc tgatataaag cgatccttgc ttcttgttcc caatcctcag aaacataatt
     4861 tcttaaataa taggtgtttt tcaaacgtag taaaagtgga aaatattttt gatacagggc
     4921 agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat aactcctcag acataaaata
     4981 attcctccat aaattcgttt atggaggaat cttatcatca atgattcatc gaactgatta
     5041 gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac aattttttta
     5101 actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata tctttatttt
     5161 cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa gcagaagttc
     5221 gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc gccactgtca
     5281 cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg tcagccgttt
     5341 cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt tggatgcccg
     5401 gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc gcataatttg
     5461 ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt ttaagctgga
     5521 caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc tgttttttca
     5581 tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca gcagcaacac
     5641 tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca tccatttctt
     5701 ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca attgatcctt
     5761 tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc caaaatcctg
     5821 tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt
     5881 cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct cggtgttttg
     5941 gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt
     6001 gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc
     6061 gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga acgactcctt
     6121 gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat ttgactggca
     6181 cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt gaatcttctt
     6241 gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa
     6301 agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga tttcgtccag
     6361 tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt ttcgattgat
     6421 gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca ccagcggatc
     6481 aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag cgcttcgaac
     6541 cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg gcttttagca
     6601 tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc ttccagcatt
     6661 tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc acgatgcagc
     6721 ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc
     6781 gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct catgcttctc
     6841 ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct ttcaacaaat
     6901 cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt cgttcttcga
     6961 tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata ccgaagatcg
     7021 aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa actttcggct
     7081 ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc ccatttgctg
     7141 cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct aacaaagcga
     7201 ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat ttcaaattgt
     7261 caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt
     7321 agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa
     7381 cgtttttgat catttcatgt acagtgatga aattccctag agatttactc attttttcat
     7441 cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat tttttccctg
     7501 ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa tcttgcccgc
     7561 caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa cattcaatat
     7621 gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca ggttttgctt
     7681 gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc cctgtacgct
     7741 gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca taattttcaa
     7801 atttacgggt acggtaatac acatcacctg aagattcata agcatagcct ttttcaacca
     7861 acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga tgcagtgtag
     7921 caggttcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa
     7981 cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg tctacatccg
     8041 tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt cgaattgtat
     8101 cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca gtcggtccgc
     8161 agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt tttctggtca
     8221 atgtattgta gatttttatc attctgatct cctcactttt tctcctttga ttcgaacgac
     8281 ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca caacggcacc
     8341 tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg cattcgcaga
     8401 gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc
     8461 taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt cgccaatgac
     8521 gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg gatggatctc
     8581 aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa agagccggtg
     8641 ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa
     8701 aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat caatcgctcg
     8761 ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg aaatcatctt
     8821 cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg ctttccgcgt
     8881 ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg ttatacgttc
     8941 ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac
     9001 tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta tctctcttgt
     9061 aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt gatttaaatg
     9121 tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg gaccatgcat
     9181 ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta cgccagtttc
     9241 tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt ccatttgttc
     9301 tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa gtacttcttt
     9361 tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat caacgatttc
     9421 tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc attcgattgt
     9481 cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca gacacatgtc
     9541 agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt gcagtttttt
     9601 cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt tgatcaattc
     9661 ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg caataaagtt
     9721 gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt gcaagattga
     9781 ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg tcatatgtcc
     9841 gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg gtgtattggc
     9901 aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat cgaccgcaac
     9961 agcaaaatta taagttggca taccatcgcg tttttggatg acaaagtcgc caccgatatt
    10021 atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt ctgtattgcg
    10081 aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg cttgttcttc
    10141 aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc
    10201 ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc gattgctagc
    10261 aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac ggtaagggcc
    10321 gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag caaggttttc
    10381 caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct caatacgaat
    10441 aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag ttcttgcatt
    10501 tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt
    10561 cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag ttacggcttc
    10621 tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa ggtaaatagt
    10681 aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat tttgattgtt
    10741 tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt gcaggtttag
    10801 caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact tcgatacgtt
    10861 cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca aggtaggcta
    10921 ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt tcaccaggga
    10981 tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt acattttgga
    11041 attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct aaaagcttag
    11101 ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca tcgtacattt
    11161 cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg cgacgaccac
    11221 gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa agaacaaaat
    11281 tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa atccgaccgt
    11341 caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct gattttcgtt
    11401 ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg cttcgtgaac
    11461 caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc agatagccta
    11521 agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc atattgtaaa
    11581 gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc gcaccgaaga
    11641 gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata atagctgaga
    11701 tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac aggctgttgg
    11761 taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa gctgagggta
    11821 aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg acacgttttt
    11881 gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca cgcttcaacg
    11941 tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc cagttaccta
    12001 agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct ttgactcgtt
    12061 gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg atgaaacatt
    12121 ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg atagctaaat
    12181 caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca tcttgattct
    12241 gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc cgattgaaat
    12301 caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact aatgcttgga
    12361 tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag ccagttgcac
    12421 cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc aatccatgct
    12481 catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt actgctcgca
    12541 aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc accatatgtt
    12601 ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg ataaaaatgg
    12661 ctatcccatt tgttttagcg gttttgagta gttctgcagt ggtttctctt acttggctga
    12721 cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa tcaatgataa
    12781 cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct gtttcagcgt
    12841 atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt atctgctctg
    12901 cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat tgctgagaaa
    12961 cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag accatagacc
    13021 ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt ttgatccgag
    13081 gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct cctgtcaaac
    13141 ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc gtattccatt
    13201 ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa ttttggcaaa
    13261 caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta atcatgagac
    13321 attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa atccgccttg
    13381 acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc
    13441 gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga attggaacat
    13501 gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac ctactccgtt
    13561 tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc
    13621 atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca attgtttttc
    13681 attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct ttttccagcc
    13741 tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat agccagcagc
    13801 atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag aaataacttc
    13861 aaatcctctt tttctcaccg gttttcctcc ctaaataaac tactcctatt ctaacatatc
    13921 cgaggctgta aaagactttc tttacaaatt cttcaagaaa ttctgctatc ttaaagtaaa
    13981 ccattaattc gttctttttc caaacgactg caagttgtac aaaaaaacta ttttttacat
    14041 gactttactc gtcaaaacaa gtagaataga acgaaattaa tgagtggata tttgattgag
    14101 gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt attgtcaaag taggaacaag
    14161 tacattgatt tatcccaatg gaaatatcaa tcttgcggca atcgatcagt tagcttttgt
    14221 cctttctgat cttagaaatc ggggaaagga agtgatctta gtctcatcag gagctgtagg
    14281 cgttggtatg cataaattac atttgtcaga acgtccgact ttgattcctg aacagcaagc
    14341 tgcggcggct gttggtcaag ccgaactgat gaatatctat aatcaaagat tcagtgtata
    14401 taatcaacag acggcacaga tcctcgtgac aagagacgtg gtagattatc ctgaaagtcg
    14461 taaaaatgtg aggaatacat tggaacagct tttgattatg gacacgatcc ctatcgtgaa
    14521 cgaaaacgat actgtagcag tggatgaatt agatcatctg acaaaatttg gcgataatga
    14581 tcagctgtca gccatcatgg caaaacttgt cgcggcagac cttttgatcg tcctttctga
    14641 tatcgacggg ttttattcag ataatccgac tacaaatccc gaggctcaga tgtatcgcta
    14701 tatcgataaa atcgatgaaa agattctggc acaagcaggt ggtgctggta gcacatttgg
    14761 aacaggcggt atgcagagta aattaaaagc cgcttctagg atatttgaag caaacagcgc
    14821 aatgatttta gcaaatggaa aaaatccggc aattattttt gatattcttg caggaaaaga
    14881 agtaggtact ttatttaagg aggacttgaa atgactgact tgatgcaatt aggacaacag
    14941 gcaaaagata gcgcgcaaca actagcactt atgcctacaa accgcaaaaa tgagctactt
    15001 gttcaaatgg cacaaaccat caaaaagaat caacaagcga tcattgaagc aaatcataaa
    15061 gacttagaaa aagcagtcga aaacaatatc agtgaaacga tgcaagaccg cttgcagctg
    15121 acacctgagc gaatcaatgc catggctaca gagatcgaaa aaattgcttc gttagacgat
    15181 cctattggaa aagtagatga gatgtggacg aacaccgacg gattgagaat cggcaaaaag
    15241 cgcgtgccac taggcgtgat cggcatcatt tacgaatctc gtccaaatgt cacgacagat
    15301 gcagctagtc tagcattcaa atcagggaat gccgttatcc ttcgcggtgg caaagaagca
    15361 ttcttctcga atcaattatt agtccaatta ttgcagcaag tactggtcag tgctggcgaa
    15421 tctccttatg ccatccagtt tgtcgatgat acttctcatg aaactgcagg acagttgatg
    15481 cgattgacag aatacttaga tgtgctgatt cctcgaggcg gagcaaaatt aatccaacga
    15541 gtcaaagaac aggcaacggt acctatcatt gaaacaggta caggaaataa tcacgtatat
    15601 atcgataaag aagcacagtt atctatggca gtgaacatca tcgtcaatgc aaaagcttcc
    15661 cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc attctgagat cgcacctttt
    15721 ttcttgccag ctattgaaaa agaactggta gaacacgggg tctctttacg tgcggatgct
    15781 cgtgcgttag aatatctaga gacggctgct ttagcagaag acacagattg ggatactgaa
    15841 tatctagatt atatccttgc tgtcaaagtg gtcgattctt tggatgaagc aattgcacat
    15901 atcaatcggc ataatactaa acattcagaa acgatcgtga cagatagtta tgcggctagt
    15961 caacgtttct taaatgaagt ggacgcggcg gctgtctatg tcaatgcctc cactcgtttt
    16021 acagacggat cggtatttgg ttttggtgca gagattggta tctctactca aaagttgcat
    16081 gcccgtggtc caatggggtt aaatgaatta acttccacaa aatatatcat ttatggcgat
    16141 ggacaaatcc gcgaataatg tttcgtgtga aacattattc ccgccttttg ccttttttct
    16201 gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg aaattttaat gatttttttt
    16261 atttacctta tttctctgaa tatcgctgct tttctgggag tcggtatttt atcccttttc
    16321 tttcaattca aaaaacgaag catagggagt caacgggaaa aatggtcgca gtattttgac
    16381 aaaatcgggc aaaaagagct ggttacacgc ttgcacatta gttacatggt tgcattatgc
    16441 ttattagcga caatcaatta ctattctttt ttcgatcatt caatcgctta tacgattacg
    16501 ctactgattg ctgggatttt tcacttatct tataaatatc agctgaataa aaaccacttg
    16561 aatcggactt tccgctaaaa aatatccgta cgataagtag agtgccttga agatcggcaa
    16621 aaaactgccg gactttttag aggctgtgac aaagattttg tcacagcctc tttttccata
    16681 taaacggcgt tcgcctacca gctgcctggt aaatctgttt cctcttcgtt aagaagcgaa
    16741 aatccgtcaa atgtttcgcc atcataataa cgaagcagct catccatgga caagttcatc
    16801 gttttttctt tttcttcttc cgctgattca gccagtagct gttcccgata cacctgatac
    16861 ataccagcta ttttttgctg catcgctttt ctagtttcac cttccgcatt gattattggc
    16921 aattctttga tatagccacg ataaatcaac cgatcatctg aaaattcaat gggtaattca
    16981 acgagtgtca acccgacttc ctgaaaatat tcttccagca tctattatcc ccccattctt
    17041 tttcttaaat gaattttagc ttacctactg aaaattttcc agtttcatta ttttgagaaa
    17101 aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt ccgaataaat ggtgttcttc
    17161 tgccaactcc tcaatcataa gtcacaatat tcaacaaaca tgagaaactg ttttttgaaa
    17221 ttgttccttg tgattcagag ttaaacgaca gtctcacaac ctcttggtaa acggcgttca
    17281 atcagctgac cctcggtatt agctcaaaaa acggtaaaat atgagaagct atttttccgt
    17341 tttgtttctc taatacttag gataaaacgc tgattttaca acctctgtta aac
//
LOCUS       DADOZM010000049        17375 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.050, whole genome shotgun sequence.
ACCESSION   DADOZM010000049 DADOZM010000000
VERSION     DADOZM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.050"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(355..1990)
                     /locus_tag="J8I47_001422"
                     /pseudo
     CDS             complement(355..1990)
                     /locus_tag="J8I47_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2545..3108)
                     /locus_tag="J8I47_001423"
     CDS             complement(2545..3108)
                     /locus_tag="J8I47_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ6322866.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(3123..4166)
                     /locus_tag="J8I47_001424"
     CDS             complement(3123..4166)
                     /locus_tag="J8I47_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322867.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4299..4703)
                     /locus_tag="J8I47_001425"
     CDS             complement(4299..4703)
                     /locus_tag="J8I47_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ6322868.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4936..5571)
                     /locus_tag="J8I47_001426"
     CDS             complement(4936..5571)
                     /locus_tag="J8I47_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAZ6322869.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5837..6889)
                     /locus_tag="J8I47_001427"
     CDS             complement(5837..6889)
                     /locus_tag="J8I47_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6322870.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6902..7840)
                     /locus_tag="J8I47_001428"
     CDS             complement(6902..7840)
                     /locus_tag="J8I47_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAZ6322871.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(8291..8941)
                     /locus_tag="J8I47_001429"
     CDS             complement(8291..8941)
                     /locus_tag="J8I47_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322872.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(9041..9550)
                     /locus_tag="J8I47_001430"
     CDS             complement(9041..9550)
                     /locus_tag="J8I47_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAZ6322873.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9751..10620)
                     /locus_tag="J8I47_001431"
     CDS             complement(9751..10620)
                     /locus_tag="J8I47_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6322874.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10640..11761)
                     /locus_tag="J8I47_001432"
     CDS             complement(10640..11761)
                     /locus_tag="J8I47_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAZ6322875.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11763..12158)
                     /locus_tag="J8I47_001433"
     CDS             complement(11763..12158)
                     /locus_tag="J8I47_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ6322876.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(12177..12974)
                     /locus_tag="J8I47_001434"
     CDS             complement(12177..12974)
                     /locus_tag="J8I47_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6322877.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12976..13764)
                     /locus_tag="J8I47_001435"
     CDS             complement(12976..13764)
                     /locus_tag="J8I47_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6322878.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13778..14254)
                     /locus_tag="J8I47_001436"
     CDS             complement(13778..14254)
                     /locus_tag="J8I47_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6322879.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14326..16983)
                     /locus_tag="J8I47_001437"
     CDS             complement(14326..16983)
                     /locus_tag="J8I47_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6322880.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(17002..>17375)
                     /locus_tag="J8I47_001438"
     CDS             complement(17002..>17375)
                     /locus_tag="J8I47_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAZ6322881.1"
                     /translation="AADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLE
                     EYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQLN
                     DLNKAFLASHIYGKILSAKFE"
BASE COUNT         5511 a         3537 c         2564 g         5763 t
ORIGIN      
        1 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
       61 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
      121 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
      181 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
      241 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
      301 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
      361 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
      421 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
      481 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
      541 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
      601 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
      661 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
      721 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
      781 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
      841 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
      901 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
      961 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
     1021 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
     1081 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
     1141 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
     1201 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
     1261 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
     1321 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
     1381 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
     1441 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
     1501 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
     1561 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
     1621 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
     1681 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
     1741 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
     1801 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
     1861 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
     1921 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
     1981 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
     2041 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
     2101 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
     2161 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
     2221 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
     2281 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat
     2341 gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga
     2401 tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac
     2461 tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc atctggtata
     2521 aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat
     2581 catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag
     2641 tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa
     2701 taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc
     2761 aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac
     2821 caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa
     2881 tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg accaatttac
     2941 acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga
     3001 atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac
     3061 atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc
     3121 gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct
     3181 aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg
     3241 ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt
     3301 ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc
     3361 gaggtataag gaccataaga acgactggct aagagataat ggccataaaa agccacacct
     3421 tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg
     3481 cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca
     3541 ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct
     3601 tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca
     3661 gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct
     3721 gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc
     3781 cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata
     3841 atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa
     3901 gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc
     3961 attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct
     4021 gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc
     4081 ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa
     4141 atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc
     4201 catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga
     4261 tctttcataa tctcatttat agtaacatta agaatccctt attctactat agtcacttcc
     4321 acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg
     4381 attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt
     4441 tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac
     4501 tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca
     4561 gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc
     4621 atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt
     4681 gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg
     4741 taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca
     4801 acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata
     4861 tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc
     4921 ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata
     4981 gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact
     5041 aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa
     5101 aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa
     5161 caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt
     5221 cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc
     5281 acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc
     5341 aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga
     5401 ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt
     5461 agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact
     5521 catcacacct aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc
     5581 ctacttttta gtgattattc gatacatcca acaatcaaat tttttctcat tttaacacat
     5641 atttttattc tagttgtctt ttgaacaatc aataacataa aggtgagttt cttctacatt
     5701 ttaaaaaatc gaatggcact gaagagttca gttgtcagca tcaatctcct actcaaaaag
     5761 gcaaaaaaac gtaagtcaga tgttgttgac atctcactta cgcatccatt gattgaaaat
     5821 ttcttagtaa aatctactag cctaaaaatt tgaagacttc ttctcgatca tacagtttaa
     5881 atactttctc gcataaaaat tgagtaatta gtgccgcaaa aacagggatg actaaccaac
     5941 aaagaacaac aagaaagcca tttagccctg catctaaaga agccaacgga ccaacaagtc
     6001 cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat agaaaataga gcgacaggaa
     6061 tagctgtaat cactgcacta actaaacaag gaataagaat cacaggtttt ctaaatagat
     6121 taggcatcat catcttcata gcgcctaaag caatcgcaat tgtcacacct gacttattaa
     6181 ccttccaaga gttcactact aaaacaaaag tggttgcagc tacgcccatc gcagccgcac
     6241 cagcagaaac accatctagt tggattgcca ttccgattgc tactgttgaa ataggagaga
     6301 taatcaaaaa ggcaaaggaa caaccaatca agatagacat taacagcggt tgtaaggtag
     6361 tgaagttatt aatcaatgat ccaattgcag aagtaattgc tgttacaaaa gggaataaat
     6421 aaaaaccaag taaacctgca cctacaccta caacaatcgg tgttaacaca atcgacactg
     6481 acccaaaact atttccaatc agtaaaatca gcccgacagc aatcgatgct gtcagcatcg
     6541 tattaataat atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc aaatcaggtg
     6601 tgaatttgac tacaccagaa ccaacaaaag aagctccggc tacaaccatc attctcatcg
     6661 gatcaaatcc aaattgtaag gcaatcaaac caccaatgat caaaggtgtt gctagttgaa
     6721 aaataactgc taactgtgcg atcgttaccg caaagggata cgcactaaaa tattttaaaa
     6781 tggcacctaa tactgcatta ggaatcaatc caataatgat tcctgttgct gtaccagcga
     6841 ggactttatt cagaaaaatt ttcggtgata ttttttgtga agtttccatt caatctctcc
     6901 tttattttac gttcaaacta tcttcttttg catggacaag ctcagtcaag aaatcacaat
     6961 aaggcgtcgc aacaccatat ttcttccctt ttctagaaat tgcgccattg atataatcga
     7021 tttctgttaa tcgatggttc ttaatcaagt cttgatacat tgaaggataa tgtaacccaa
     7081 ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca tctagttcaa
     7141 tcttctctac tgctgctact ttggcaaact cattgacaat cgtcgccacc attttatgag
     7201 cagtcgatgt ttttcctagc tcagccatat taacgtctaa gattgtgcat agtccgttca
     7261 ttgttccgtt cacacatgct ttgcgataaa tagaatagtg aatattgtca gaaaaatgag
     7321 catttaatcc tgattcagac aatttgtctg ctaatttttt tgcagctgct tcttttccat
     7381 cacctaaatt ttgtaattct actgaaccac ttccaaataa tttgacctga ccaggacctt
     7441 caagacctgc cgtccacatg gtatttccaa tatagatatt ttccattggt acaaatttct
     7501 caataatatc ttcatgcccg ataccattta atagacataa gacctctgta tcttttttga
     7561 ttaatgattg gatatcctgc agcatttttt ccagctgcat cgctttggta aatagaataa
     7621 tcagatcaac ttgatcttct ttttctactt cggattgaag aacgattggt agttttgctt
     7681 ctacctcttt accattaaaa tttgcttgta atccatgttc tttgatttgt tggacatgtt
     7741 ctgcccagcc atcgattaat aatacttcgt tcccactttg gtgaagcatc aacccaaagc
     7801 gacttcccat tgcgccagcg cctgcaattg ctattttcat tgattcttcc tcctttttct
     7861 ttctattata ctcatttgag aatcttttca caacctcgtt ttgttattct tctcttattt
     7921 atcatgttta tatccaccat tcaagttatt ttatcagcaa atcaataaaa actggaatag
     7981 aatcccaatt tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag
     8041 aaaaaatctc actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc
     8101 ttccattcat tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt
     8161 tgcgcaacac agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt
     8221 taaaaacttc tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa
     8281 aaatagcctt ttattgtcta tgggaataat aatttaaaat tggtactttt ttattaatta
     8341 gttcatcgcc caataatctc tccagtttct tagcatctcc tgactcaacc ggaattatcc
     8401 aagcccagtc taaataattt tttaaaatct catcatattt tgcaaatatt tcattgccat
     8461 attccgtcct taaaattata ttttcagtgc cagacaagtg atttatattg agttgataaa
     8521 aaagatctcc atctaaaaaa atatcattag aagaagattt ccccacagta atgaccattc
     8581 catcagctct atgtgttaca tacaaataat tgtatgacct catttcacct aatgaatcaa
     8641 ttatttcttg ccctttagct ctaatatatt gatctctatt acctatctca ctcttattta
     8701 acaaaagttt tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac
     8761 tactaatcca atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg
     8821 ctaaatactt tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac
     8881 atcgattctc tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca
     8941 taaaaccaat ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct
     9001 catcaaaaca accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat
     9061 aaattcctaa ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc
     9121 gttggccttc ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct
     9181 cagcaaaaac gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta
     9241 gcaacacatt gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct
     9301 tatctttttg cggaccacgc gccaagagaa tcaggccatt taggaatgcg tcaaaaactt
     9361 ggttgttgat ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta
     9421 ctttggtcag taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag
     9481 ctttaatcaa atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat
     9541 tgttattcat ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt
     9601 gctaagattt ttaggatcaa tgataaaaac cgcatcactt atgataaggc gctgggtatc
     9661 atgagtgaat gatattgctg aatacaaata gtccgaccac attaagttga tcggacttca
     9721 taagcatttt attgactaat acttttttgc ttaagaattg attctaacat cttttgctaa
     9781 agttaagcat cctatagttc ctgcattatc tgctaactta ggtgtgacaa tgtattcttc
     9841 gatatttggc gtatcgacat agccgttaat tagttctaaa aaacgtcttt gtactttctt
     9901 tttcagatgt tcttgttgca tgacaccacc accaaaaatt ataatatctg gtgaaagcat
     9961 cagggtcgta ttacaggcac attgtgcaat ataatccgcc tcgatttccc agaacggatc
    10021 ttctatagaa agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc
    10081 caacccttcg agacaatcat gatgaaaagg acaagtacca gagaaagtat ctcctgaatg
    10141 tctcttaact aacgtatgcc ccatttctgg atggctaaac ccttcaacaa aaatcccatc
    10201 ttgtatagct cctccaccaa ttccagttcc aacagtgtaa taaacaacac tggataaacc
    10261 ttttccatga cctgaaacat actccccgta acaagcagca ttcacatcgg ttgtccaggc
    10321 aattggaatt tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt
    10381 ttttggagta gaggtgatat gtccataggt ctttgattca cgatggatat caatcggtcc
    10441 aaatgaccct actccaatgc tttctaattg ttctttatag tgattaaaga aatcaattac
    10501 caaactcatt gtttcattcg gtgtagttgt aggaaaactc actctttcta aaacctttaa
    10561 atcatcattg ccaatggcac aaacaaattt agtcccacct gcttctattg agccatacat
    10621 ttcttactcc accgtccttt tatctaggaa ttcgttcttc cctgtctaat agtttttcga
    10681 aaaactcatg tggttccttt tggtaccaat aggcaacaga agaaatatcg tcttgacgct
    10741 cataaagctt gatgtcatca tttcctaggg tttggaaagt aacacgtaaa ttttctttga
    10801 aactaatggg gtcgggtaaa tgccagcggt acaacccatg catcggtaaa gcatcatcgc
    10861 caaaaccatg aactggattt ggattaggtt ttccagcact aaaataatcc cgggtgtgat
    10921 ctcttttaga ttgaaaagga tagcccataa atagcgtatt aaaattttga gcatatgccc
    10981 tttctccaaa gttcttttta tggaaagccc aagcgccgcc aaaataatct tctgctcctg
    11041 tagatgttat tgtagggaaa tcttcatctc catctatata aaacttaaat tctccttccc
    11101 cccaccagta tctttccaat gcgcacaaag caaagtaggt tcctacataa tacccagtgc
    11161 ctttgattcc atcaataagg gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc
    11221 atttagcatg aaaatacatc gcatcatccg gtacttcagt caatgcataa ttaaatgtat
    11281 aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa agtaatctta gctttttttc
    11341 gaaatggcat ctcaaaatag caattcatcc ctgatgttgg attcactacg attggtaagg
    11401 aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat ggactttcta
    11461 cagaaggagt ggattcatca tcccaataaa tacgcaaaat taaatcacgt aagacgaaac
    11521 tcccagcgat tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt
    11581 ccatttctgc taaagtaatt gtctctccag catttatcgg aacagaaggt gacccttttc
    11641 tcccaattcc tagatgactc gctgtcattg ccgctttccc tttttcacca tttttatttt
    11701 ctggtgaaat agttctactt tcctctccct tgtggagcca tatattattt aaacaattca
    11761 ttttattcgt cctctatcct ttcttcactt ttttgattat tcatatagat gatttgttgt
    11821 tgtgcctccg caataattgc tccaatatct aaattatcat ttcctaactg caaagcgatt
    11881 gacattgcca agggaagatt cattcctgca attacgtgca ctctgtcaga aatataggta
    11941 aaaaactttt gatttacact tcctccagcc atgtcagtta aaacaattat ttcgtctgta
    12001 gtatccacta actctttaat ttgttcatct atatcatcaa tttcttcgac atatgcacat
    12061 aagctactaa tttgattatg catacctgtt aaaaactcta gtgtctcttt aaaaccctca
    12121 gctagtattg aatggctagc aatgatcaat cttctcataa ttcaaatcct tttcctttac
    12181 gctaatattc caaatgcagc cataaccatc gcaaaaacaa taacgagtag aatcaaaatg
    12241 gtcatactga tttttttctt actgagtaga taatatgctg cagcagtaat aattactgga
    12301 attaatgttg gtaaaatttt gtccaacatt ggttggattt tcatactaac ttcaccaaaa
    12361 gtgaatttaa gtggcgttga aacactgata actgaagcaa ttaatgatcc aactactgtt
    12421 agccccaata ctgaagcagc ttcagttaaa taattaatct tttcaccgta ttcagtaaca
    12481 atcttcattc cctgcttata accaaattca aagcctctca ttcggaaaaa gaagattacc
    12541 atgattaaaa tgaaccataa aaagattcct acaggattat tttctaaagc catataagcc
    12601 gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc aataccagca
    12661 attggaccca tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc taatccttgc
    12721 ttttcctcca ttgcaacaca agcacctaaa ataatggctg ccagccaagg ctgtgtattg
    12781 taatactgaa agtgattatt taaagcttcg atgtattcat catcatcttt gtataatttt
    12841 cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg ataattaaag
    12901 gtacaaactg acatcaacca acgatagttc gctttcctaa gatccttttt cgttacttga
    12961 tatttttcgc tcattttatt catcctcccc aataaaccca acatcagaag ctcccgcaga
    13021 agcagtgatt tgtgcatctt tttcagctct atgatacaca ttaaatgcta aagcgaatcc
    13081 tatgatcgcc gctccaataa taggtacctt caaaaaagcg gctaagaaaa atccaacaag
    13141 taaataacta atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc
    13201 aggcaacatt tttccggcta atgttagccc atccataaac caattaggta aaacatccaa
    13261 aacgtaatta acagcagact gtcctaaagt aacggcaata aatactggaa ttgctgcaga
    13321 taaaccaaag aaaattggcc ccaaccacaa aacactattc attttcttaa attctcgttt
    13381 attcgcatat tcctgtgatt tgcgaacaac aaaaccattt aagattttga caattacatc
    13441 tagttgcacc cctaacatac ctacaggtag tcctattgct aatccggctt ctactccaag
    13501 ctggctacca aatgccgttg caataattgc gcacgttcca tagtccggta cagatgagcc
    13561 accaaaaccg gcaactccta atgacatcaa ttgaactgta ccgccaacta tcaaacctgt
    13621 ttttatatca cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa
    13681 gatcatcgga atcccataat aatcaacatg tttaataaat gctaatatag tcagcataat
    13741 aatttgaaaa atagtaaaat gcattatcct tactccttta tcctaatttt gattgaattt
    13801 cctgtgcctt atccgccgga acaaattgtg taacaatttc tacaccagaa tccaacaatt
    13861 ttttatacgt gctgatattt tcatctgatg cataggctct agaagatagc tgtaatgcat
    13921 tatctttaac aacagtaccg ccaacaatta gtcggtcaat tctaataccg ttatctacta
    13981 aatgaaggat tgtttccggc tctttcacga tgacaaatac tttttcctga tcgtatttcc
    14041 cctttttaaa attcgcaatc gctgtttctt cagtgataat tgatacagcc attccagcag
    14101 gtctagctaa acgcatactt gatttttgga tgtcgttatt tgcgacagaa tcatcgatca
    14161 ccatcactct gtttgcttta gacattggtg cccattgcgt gacaataatc ccatgcaata
    14221 aacggttatc aactctcgac ataacaatac tcataaatac ttcctccttt atctttcatt
    14281 ttcttacaag caattaccat gccaaaacaa aaagaacttg ataaatcaag ctctttttac
    14341 ataatcataa acgtatttaa cctcatgaat cgggatgtcg acactataat aatgttcaac
    14401 gacactgaaa gattgcttaa aatgtgtctg aaatttttga aattcctcac tcgtttcatt
    14461 gaaataaatt tcatcttcca aagtattttg ttttactaat cgttcaatta agcaactaat
    14521 gtgaacatat aatccgaaac acgtattatt atcaaatgaa attcctagat cattttgtaa
    14581 gatgtatata gcatctgaaa cccgttctaa taatttagtc ggatttaata tggtcaattg
    14641 ctccattaaa ttcgttagac taaaattctt cagaatatta ttgttcaaaa ctttcagttc
    14701 attatcatta aagtgtctat tcaattgatt ggagaattta cttatattca ttcctaaaat
    14761 catatcttca attgaaaaga attctacatc ttctagatgt ggatttagtg ttccaacaat
    14821 aaaaagaata ttgttctctt tacgaagctc cgataaatag ttttcattca ctaaagagta
    14881 atagtcacac gtttctatga caagatcagt atcccttgga aagctatctt caagtatctt
    14941 ttttaattta aacgaagttc ctaatccgct tgcacaagaa cagacaatca tatccttctt
    15001 aatatttgat gattcataaa tataatccac attttgattt tcttttactt gttcaagaat
    15061 ctgtttaagc ggtaaatcta ataatatctg atttgcaaca tcaatagcca ttcttgttga
    15121 aacattgttt agtagtccga attttttttg agcgtctagt tccaaattta gataaatttt
    15181 ctccagtgaa cccatatcaa caagtaaaat aatttcttca tatccggtac actcttgtaa
    15241 gtatctatta attttgtctg caatctcact acttgaagtc attagtggca tatcaatggc
    15301 gtcgtatata tatctctcaa cgatcctatt agcagtactt gctatacagg ttgccgttag
    15361 atctccatga gccaaaatca gtgctaaacg tttttgctga aattctttac tacacattaa
    15421 cttaaataaa ataaccaaaa catttttttg aatttttgtt aaataaatat cccctatttg
    15481 aataaaacga ttgactaatt gaacttctcg actacaccaa ctattaatat cattgatgaa
    15541 ggaatataat ttatcgtccg atgttaattg gatttgataa atccaagaat tcaaaaagat
    15601 agacgttatt tgaagataat aatagtcaaa ttctatttga aaatctctct ctatttcctt
    15661 aacatactgt ctaaatcgaa tctcttgata ggtgggatat gttaaagctg aattattttg
    15721 gatatcaaca ttcttttgtc taaaatatct atcatatttc tcaaaaagct gctgcttatc
    15781 agtgtctttc ttccacgttt gataaagata ctctataagt tcgttgatat taaaaacttc
    15841 gtctactttt tccaaatcat ccatttgaat atattttaat tcattctgtc ttttcataac
    15901 cttcatgccg ttgaaattca cccattgtgg taaatgatac atctgaatat aaagttcttc
    15961 caaatcatct aagaaataaa aggcattggc acaggtcaat tgaatgatat ttttcaaatt
    16021 gccaacattt tcactaaagc gatagtttaa taaaaaatct aataattgtt gagatatcag
    16081 tattttcttt gctagtcttt cttcttctga tttaaaaaat atcttgatta actgttttct
    16141 ttcataaaga gaacgactgt ctaatgttgg aagtgttagc tttacaggta ttcttctcaa
    16201 caaagttgat aatagtttat cctcaggtgg ttctgatgtt gcaaatataa tccagcaact
    16261 tgatgtgtac cagttttcat tatcacccat ccgatgaaat ttcctcgtat ccataaactg
    16321 aaaaagtttc tcctgacatt cattgctcaa tgcatgaatt tcatctaaaa ataagactcc
    16381 attattagct aattctgcta aaccctttct tccatcttcg gctcctgtat atgcaccttt
    16441 tacatgccca aaaaaattgg acaagaaaag ttctggatta ttggcatatt cactacaatt
    16501 aactgaaatg aaagatgcct tctcttctat ataattattg gccaacaaat ccttatacac
    16561 ataatttgct aaatagcttt ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc
    16621 atttggcgga tagtagattg ctgctttaat ttgagcaatt acatttcgca gacttccttg
    16681 acttccgata accctttcta ttccaaccct aaaagagcta tcataccatt tttccgcttc
    16741 ctctgaagag ctaaaaattt gttgatttgg aactttctcc ggtaaagaaa agaagattac
    16801 tggacgagta ttgatttttc caaattctcc atcttttacg aattcattca aatattgaga
    16861 gacaatatta cgactaacat ttaatttttc agatattttc tgtgctgtat tgttccccca
    16921 tttattttta atctcagagt tctctatgac ataattgaca atgcgttctt ttaattcttc
    16981 catcacagca cctcctcgac actattcgaa ttttgccgac agtatttttc cataaatatg
    17041 acttgctaaa aaggctttgt ttagatcatt caattggaac gttgcttgtt ttgcttgatc
    17101 cgctaagacg ttgaaaagat actctggcga aatcgcatac cccaaaatat caacgattgt
    17161 tgcaatcaga tcttcttttg attcttcatc tgaaagtaat tcacgataca acatcgtttg
    17221 tttgcttact gtgtcatatt cctctaagga ttcatctgct aaaagaacca cttgttccaa
    17281 caaacggtcg gataaatatt tatagaaaat cagccccaat aaataatttt tatattctga
    17341 cgcatccatt ttactacgga gattatctgc cgcac
//
LOCUS       DADOZM010000050        17355 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.051, whole genome shotgun sequence.
ACCESSION   DADOZM010000050 DADOZM010000000
VERSION     DADOZM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.051"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(27..725)
                     /locus_tag="J8I47_001439"
     CDS             complement(27..725)
                     /locus_tag="J8I47_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAZ6322882.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="J8I47_001440"
     CDS             complement(718..2070)
                     /locus_tag="J8I47_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAZ6322883.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="J8I47_001441"
     CDS             complement(2293..3693)
                     /locus_tag="J8I47_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAZ6322884.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="J8I47_001442"
     CDS             3854..4591
                     /locus_tag="J8I47_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAZ6322885.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="J8I47_001443"
     CDS             4716..5195
                     /locus_tag="J8I47_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAZ6322886.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="J8I47_001444"
     CDS             5192..5959
                     /locus_tag="J8I47_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322887.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="J8I47_001445"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="J8I47_001445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ6322888.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="J8I47_001446"
     CDS             complement(6862..7464)
                     /locus_tag="J8I47_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ6322889.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="J8I47_001447"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="J8I47_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="J8I47_001448"
     CDS             8145..8729
                     /locus_tag="J8I47_001448"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAZ6322890.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="J8I47_001449"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="J8I47_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAZ6322891.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="J8I47_001450"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="J8I47_001450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAZ6322892.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="J8I47_001451"
     CDS             10630..10809
                     /locus_tag="J8I47_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322893.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="J8I47_001452"
     CDS             10831..11856
                     /locus_tag="J8I47_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAZ6322894.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="J8I47_001453"
     CDS             12031..13275
                     /locus_tag="J8I47_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAZ6322895.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="J8I47_001454"
     CDS             13428..14375
                     /locus_tag="J8I47_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6322896.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="J8I47_001455"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="J8I47_001455"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAZ6322897.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="J8I47_001456"
     CDS             complement(15277..15519)
                     /locus_tag="J8I47_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322898.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="J8I47_001457"
     tRNA            15640..15712
                     /locus_tag="J8I47_001457"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="J8I47_001458"
     CDS             complement(15831..16469)
                     /locus_tag="J8I47_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ6322899.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="J8I47_001459"
     CDS             complement(16592..17224)
                     /locus_tag="J8I47_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAZ6322900.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5918 a         3077 c         3440 g         4920 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaat
//
LOCUS       DADOZM010000051        17346 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.052, whole genome shotgun sequence.
ACCESSION   DADOZM010000051 DADOZM010000000
VERSION     DADOZM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.052"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            2..369
                     /locus_tag="J8I47_001460"
                     /pseudo
     CDS             2..369
                     /locus_tag="J8I47_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            549..1490
                     /locus_tag="J8I47_001461"
     CDS             549..1490
                     /locus_tag="J8I47_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAZ6322901.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="J8I47_001462"
     CDS             1559..2290
                     /locus_tag="J8I47_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAZ6322902.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="J8I47_001463"
     CDS             2434..4614
                     /locus_tag="J8I47_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAZ6322903.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="J8I47_001464"
     CDS             4634..5116
                     /locus_tag="J8I47_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAZ6322904.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="J8I47_001465"
     CDS             5132..5893
                     /locus_tag="J8I47_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAZ6322905.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="J8I47_001466"
     CDS             6024..7358
                     /locus_tag="J8I47_001466"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAZ6322906.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7786..8271
                     /locus_tag="J8I47_001467"
     CDS             7786..8271
                     /locus_tag="J8I47_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAZ6322907.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8264..8947
                     /locus_tag="J8I47_001468"
     CDS             8264..8947
                     /locus_tag="J8I47_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAZ6322908.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9073..11718
                     /gene="polA"
                     /locus_tag="J8I47_001469"
     CDS             9073..11718
                     /gene="polA"
                     /locus_tag="J8I47_001469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAZ6322909.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11763..12599
                     /gene="mutM"
                     /locus_tag="J8I47_001470"
     CDS             11763..12599
                     /gene="mutM"
                     /locus_tag="J8I47_001470"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAZ6322910.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12563..13192
                     /locus_tag="J8I47_001471"
     CDS             12563..13192
                     /locus_tag="J8I47_001471"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAZ6322911.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13244..13576
                     /locus_tag="J8I47_001472"
     CDS             13244..13576
                     /locus_tag="J8I47_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAZ6322912.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13888..14379
                     /gene="nrdR"
                     /locus_tag="J8I47_001473"
     CDS             13888..14379
                     /gene="nrdR"
                     /locus_tag="J8I47_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAZ6322913.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14402..15775
                     /locus_tag="J8I47_001474"
     CDS             14402..15775
                     /locus_tag="J8I47_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAZ6322914.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15775..16701
                     /gene="dnaI"
                     /locus_tag="J8I47_001475"
     CDS             15775..16701
                     /gene="dnaI"
                     /locus_tag="J8I47_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAZ6322915.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16830..17270
                     /locus_tag="J8I47_001476"
     CDS             16830..17270
                     /locus_tag="J8I47_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAZ6322916.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5909 a         3032 c         3821 g         4584 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttcccgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt tttcttggct gttcttatta cttgatgcag ggcagctttt tcacgacctc
     7561 agataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa tgaaaaatat
     7621 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7681 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7741 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7801 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7861 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7921 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7981 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     8041 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     8101 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8161 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8221 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8281 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8341 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8401 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8461 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8521 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8581 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8641 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8701 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8761 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8821 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8881 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8941 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     9001 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     9061 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9121 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9181 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9241 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9301 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9361 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9421 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9481 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9541 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9601 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9661 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9721 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9781 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9841 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9901 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9961 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
    10021 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
    10081 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10141 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10201 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10261 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10321 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10381 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10441 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10501 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10561 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10621 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10681 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10741 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10801 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10861 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10921 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10981 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    11041 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    11101 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11161 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11221 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11281 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11341 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11401 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11461 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11521 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11581 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11641 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11701 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11761 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11821 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11881 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11941 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    12001 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    12061 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12121 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12181 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12241 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12301 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12361 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12421 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12481 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12541 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12601 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12661 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12721 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12781 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12841 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12901 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12961 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    13021 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    13081 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13141 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13201 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13261 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13321 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13381 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13441 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13501 tgaccacatt tccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13561 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13621 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13681 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13741 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13801 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13861 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13921 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13981 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    14041 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    14101 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14161 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14221 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14281 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14341 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14401 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14461 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14521 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14581 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14641 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14701 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14761 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14821 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14881 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14941 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    15001 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    15061 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15121 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15181 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15241 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15301 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15361 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15421 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15481 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15541 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15601 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15661 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15721 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15781 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15841 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15901 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15961 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    16021 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    16081 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16141 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16201 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16261 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16321 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16381 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16441 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16501 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16561 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16621 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16681 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16741 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16801 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16861 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16921 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16981 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    17041 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    17101 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17161 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17221 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17281 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17341 atggga
//
LOCUS       DADOZM010000052        17052 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.053, whole genome shotgun sequence.
ACCESSION   DADOZM010000052 DADOZM010000000
VERSION     DADOZM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.053"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            114..1283
                     /locus_tag="J8I47_001477"
     CDS             114..1283
                     /locus_tag="J8I47_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6322917.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1857..2426
                     /locus_tag="J8I47_001478"
     CDS             1857..2426
                     /locus_tag="J8I47_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAZ6322918.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2413..3273
                     /locus_tag="J8I47_001479"
     CDS             2413..3273
                     /locus_tag="J8I47_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ6322919.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3463..4167
                     /locus_tag="J8I47_001480"
     CDS             3463..4167
                     /locus_tag="J8I47_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322920.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4172..6007
                     /gene="walK"
                     /locus_tag="J8I47_001481"
     CDS             4172..6007
                     /gene="walK"
                     /locus_tag="J8I47_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAZ6322921.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6004..7320
                     /locus_tag="J8I47_001482"
     CDS             6004..7320
                     /locus_tag="J8I47_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322922.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7321..8190
                     /locus_tag="J8I47_001483"
     CDS             7321..8190
                     /locus_tag="J8I47_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322923.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8245..9054
                     /locus_tag="J8I47_001484"
     CDS             8245..9054
                     /locus_tag="J8I47_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ6322924.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9157..9786)
                     /locus_tag="J8I47_001485"
     CDS             complement(9157..9786)
                     /locus_tag="J8I47_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAZ6322925.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9956..11248
                     /locus_tag="J8I47_001486"
     CDS             9956..11248
                     /locus_tag="J8I47_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6322926.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11324..11986)
                     /locus_tag="J8I47_001487"
     CDS             complement(11324..11986)
                     /locus_tag="J8I47_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ6322927.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12211..12495
                     /locus_tag="J8I47_001488"
     CDS             12211..12495
                     /locus_tag="J8I47_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAZ6322928.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12546..14171
                     /gene="groL"
                     /locus_tag="J8I47_001489"
     CDS             12546..14171
                     /gene="groL"
                     /locus_tag="J8I47_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAZ6322929.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14260..15408)
                     /locus_tag="J8I47_001490"
     CDS             complement(14260..15408)
                     /locus_tag="J8I47_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6322930.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15510..16094)
                     /locus_tag="J8I47_001491"
     CDS             complement(15510..16094)
                     /locus_tag="J8I47_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAZ6322931.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16221..16652)
                     /locus_tag="J8I47_001492"
     CDS             complement(16221..16652)
                     /locus_tag="J8I47_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ6322932.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16670..16990)
                     /locus_tag="J8I47_001493"
     CDS             complement(16670..16990)
                     /locus_tag="J8I47_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6322933.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5640 a         2876 c         3447 g         5089 t
ORIGIN      
        1 taaaaaagct gtcattgagt atatattgaa aatacaagat gaaggtaaaa atcatgctat
       61 aataaaatag tcaaaatttt tgccgatatt tcagtttaag aggagagatt gaattgaaat
      121 taagagcaaa cataattgaa aaaagaggaa cattacaagt agttgtatct tataaagact
      181 ctaacggaaa aattagacaa aagtggaaag atacaaaatt aaaaaagaaa ggaaataaaa
      241 aacgagcaga aaaaattaga gatgaatttt tactagaatt ggaaagtcaa ttagctatgg
      301 aatgtgaatg tgctaatcca aatttattgt tttatgacta tttgcttaat tatttggaag
      361 tatcaaaaaa acaagtagca tttaacacat atgttgggta taagcattac gtccataatc
      421 gaatttacaa gtttttttat cctaaaaaaa ttaaactaaa taggttaaag ccttatcatt
      481 tacaagagtt ttatcaatat atgttaaatg ataactgtac agcaaatacg gttattcatt
      541 atcacgcatt tatacgcaaa gctcttcaag aggctgttat tacagaattg ataagtacaa
      601 atgtagcaga taaagtaaga aagccaaaaa agaaacaatt tgtttctcaa gtttataatc
      661 aacaagagat cttgaaatta cttgacattt tgtcagaaga aaaactctat ttggtagttc
      721 ttttaacagc attttatgga ttgcgaagaa gtgaagtttt aggattaaaa tggtctgcta
      781 ttgatttttt aaacaaaaaa atcatgataa accatgtaat catagaaaat ccagagaata
      841 tgtcacagtt aataaagaaa gatcaaacaa agaactcttc cagctatcgt acattacctt
      901 tggttgaaac gattgaacga gcattagtaa atcaagctaa atggcaacaa gataatcgta
      961 ttcttttagg agatgattat attttagaag ataatgagta cgtatttact atggaagatg
     1021 gacggttgat gaagcctcaa tatgtaacgc atcgtttgag taagttgatt aagaaaaatg
     1081 gattgaaaaa aattcgcttt catgacttgc gtcattctaa tgcttcaata atgttagata
     1141 gtggccagaa tatgaaatcc attcaagaat ggctgggaca tgcatcgtat tcgacaactg
     1201 ctaatttata cacgcattta accgcaggag taaaagatcg tgcagcagaa gcattagaaa
     1261 acgcatttgg gtttaaagaa taaaaaaaca aaaaacaata tctttggggt atcgcttctt
     1321 gtttttagaa tttttttgtt ggcaatttgt tggcagtatg ttggcaatga aaaaattcac
     1381 aaactaaaaa cctatttata aagctttata aaactttttt cacaaataga gcatttggtt
     1441 aagagtcagc tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt
     1501 agcactaaga attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt
     1561 ccttatagga aatcattcat agattattca catttgctcg ctattatata agcataccaa
     1621 caaacaaagt tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc
     1681 cgccgtctcc ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc
     1741 ttgttttttg ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg
     1801 aatgtttatc ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg
     1861 aaaaaatgcg tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc
     1921 actgggaaga actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc
     1981 gttatctttc acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa
     2041 acaattacgt taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac
     2101 acgtagcatt tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca
     2161 aagaagggat attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt
     2221 gcgatgaaca agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc
     2281 cgcaaaaatt cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat
     2341 cgtttgcaat gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag
     2401 aggagaaaac aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtt
     2461 ccagaagaat tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa
     2521 gatcaggtat atacagataa aattgacatt acccctgaag agatctatca acgtttgcca
     2581 gaggaaatcc ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa
     2641 atcaaaaaag acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg
     2701 acatataatg tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta
     2761 gatacgaaaa atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg
     2821 gaagaaggag tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca
     2881 aaagttttct ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta
     2941 gttgcttcta ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa
     3001 gggatttatt acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt
     3061 gcttacgtca aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg
     3121 gatgcgaaag aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca
     3181 aaccaaatct actttggcca gatttctccg gcattagtag tccacactgg tccgggatta
     3241 ttgggtgtcg gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa
     3301 aatcagtgtt ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt
     3361 tcattttaat tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta
     3421 taatgagaag gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt
     3481 gtcgatgatg agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat
     3541 gacgtttata ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct
     3601 gatttgattt tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag
     3661 gtacgcaaaa cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat
     3721 aaagtcctag gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga
     3781 gaattagttg cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca
     3841 gaagaggcag cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac
     3901 atggtgacga aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt
     3961 tacttggcga agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg
     4021 ggttatgatt attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa
     4081 aagatcgaag ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac
     4141 tatttgcgta acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc
     4201 agtcaatttt aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat
     4261 tggtgcctac tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat
     4321 gaatcaaacg gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa
     4381 tgcagatgat gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag
     4441 cgatatcatc gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt
     4501 caatgaacaa agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac
     4561 taaacgttat gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat
     4621 cctgtccccg accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca
     4681 aaaatattcg gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc
     4741 atttatttca atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga
     4801 aatgagagaa caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca
     4861 tggacaagat gaacttggac agctggctga taccttcaat cagttagctg aacgtattga
     4921 agaagctcaa gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat
     4981 gacagacggc gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc
     5041 tgtttccttg ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt
     5101 agatatcgaa gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact
     5161 tgaccgttca accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat
     5221 gatccgtcgc gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga
     5281 acaagaaaaa acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg
     5341 tacaccgttg acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga
     5401 cgaggaagta gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg
     5461 aatgatcaat gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt
     5521 agaatatgtc aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt
     5581 gggaaatcag gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt
     5641 cgaagtagat acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa
     5701 gtattcacca gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtgat
     5761 tttaagtatc accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga
     5821 gcggttctat cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt
     5881 agctatttct agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga
     5941 aggaaaaggg tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg
     6001 ggaatgagaa tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc
     6061 atatacttct cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat
     6121 gagacaaaca ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt
     6181 ccgcttcatg ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc
     6241 gtgtaccgat tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt
     6301 tcaggagaca aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc
     6361 tataatgcgc cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg
     6421 tcgaacttgg caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga
     6481 aataaaaaaa tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg
     6541 ttgaattgga cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg
     6601 acaacagaac catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaag
     6661 aagtatagtt atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag
     6721 aaaccagata gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat
     6781 gaaacaatgt ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag
     6841 aaaaaagaaa atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt
     6901 tctttaggga acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc
     6961 gtggaaggct tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc
     7021 ggtgaaacga atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg
     7081 ccgatacctt cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta
     7141 aaagctaagg gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa
     7201 aatgtcaaag aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa
     7261 gataactggt attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa
     7321 ttggatttca aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt
     7381 ttattcagca gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag
     7441 actgaatcaa tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg
     7501 tctgagaaca agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta
     7561 gcgaattatc gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt
     7621 gatgttttga cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat
     7681 aaaattagta gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat
     7741 gattatgtgt acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca
     7801 agccaagagt atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta
     7861 gaaaaaaacg ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc
     7921 gagcagctgc gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac
     7981 atcaataaca aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt
     8041 attctgaaag tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca
     8101 agcgataaag tggtccaaat tgagagcgtc aacgctttca gcaatacgat cgtgacaaac
     8161 aatactgtac caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa
     8221 agtaagaagg gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc
     8281 ctagcaagtg gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg
     8341 gtcgatgcag gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca
     8401 ccggaccaat tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg
     8461 ggtgtcttgg ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg
     8521 atggaaccac tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa
     8581 gtcagaacat tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct
     8641 ccgcaatttt atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga
     8701 tattgcagtg attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt
     8761 aatcatgatc ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt
     8821 ttaggagata gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc
     8881 ggtaatcgga ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa
     8941 cttgctcatt taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat
     9001 tttgaaatct atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt
     9061 caatcaataa aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta
     9121 tttttatttt tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg
     9181 ctgcttttca tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat
     9241 gctgatcgta ctgtcgctag cagcgtttct ttgtaaataa tcatcactta acaaataagt
     9301 acctgtacgt ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag
     9361 atttttagat tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa
     9421 gggtgcattt ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa
     9481 cgaagaggca cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa
     9541 atgggacaaa gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac
     9601 gacaacatca aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa
     9661 cggaacatct gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct
     9721 ggacaatgct tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat
     9781 tttcataaaa aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc
     9841 aaagaatccg atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg
     9901 tatagttaaa ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa
     9961 agagtggtta gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt
    10021 aactgctttg cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt
    10081 tttcttattt gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt
    10141 ttctatgtat tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt
    10201 ttttgccatt gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat
    10261 aacagccttg tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt
    10321 ctggacaggt gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg
    10381 ctatgcacgt ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa
    10441 tgtttggcag tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat
    10501 cggttcagcg gtttttcaac atttgttcag aatgactgtt gttcaaggag tatggacata
    10561 tgtccagctt cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt
    10621 ttttgttccg tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt
    10681 tagtcccgtc ttgcaagcga attcattagc aaatgcagaa atcttagcct caggacaaga
    10741 gttgacggtg gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac
    10801 ggttacgggt gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc
    10861 gaagaaatac agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga
    10921 atcaattatt ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt
    10981 cgcaccggtc gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt
    11041 atttacagca gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg
    11101 cggagtgcct gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt
    11161 cccatttttg aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga
    11221 tggagcagca gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg
    11281 gaaaatcacc aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt
    11341 ggacagctaa ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg
    11401 tccagatccg accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga
    11461 ttagcagatg accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga
    11521 cccaaaaatt tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt
    11581 ccatgatggg tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct
    11641 ggatgatatt ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca
    11701 tgacagcgat gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa
    11761 tctttggagc agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa
    11821 aataaaaaat agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca
    11881 attcagacgt agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta
    11941 gaccatagca aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt
    12001 taagggataa caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt
    12061 tcaataagca gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga
    12121 gggagaatga gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga
    12181 ttaaaaaaag agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt
    12241 atcgaagtcg caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct
    12301 aaagagaaac cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac
    12361 ggtgaaaaag ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc
    12421 ggtacagaag taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg
    12481 gcgattgttg agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat
    12541 caatcatggc aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg
    12601 tagataaatt agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac
    12661 tagaaaaatc ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa
    12721 tcgaattaga agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa
    12781 aaacaaatga tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg
    12841 ttcgcgaagg tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga
    12901 tcgaattggc aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt
    12961 caaaagaagc cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt
    13021 tgattgctga tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa
    13081 aagggatcga aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat
    13141 cacaatatat ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct
    13201 tgatcacaga caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct
    13261 tgcaacaatc acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa
    13321 ctcttgtatt gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt
    13381 tcggcgatcg tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa
    13441 ttactgacga tctaggttta gaattgaaag atgcaacaat cgaaaactta ggaaatgctt
    13501 ctaaagttgt tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag
    13561 caatcgaagc tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg
    13621 accgcgaaaa attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag
    13681 ttggtgcagc tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga
    13741 atgctacacg tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga
    13801 acgttatcag caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga
    13861 tcgttgttcg tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag
    13921 gatcagtgat tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta
    13981 ctggagaatg ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt
    14041 ctgccttgca aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg
    14101 cagataaacc agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg
    14161 gtatgatgta gtaattaaga aagaacccta ttttaacagg gttctttctt ctttttttgt
    14221 tttattttaa gcaaaagggg caaagaaggg gcaaaaatat caaaggctat ttagtttatc
    14281 aattacttgt tgtttggctc tttttgttac atggttataa atagacagag ttgtgtttgc
    14341 atcagaatgc cctacacgct ccataatggc tttaagaggt acgcctaatt cagataatag
    14401 tgaaacgtgg ctatgtctga atatatgtga ggatagactt ttttctaatt ccaactcttc
    14461 ctctacttta tggagtatag cgttaaatga gtgcagcgca agcggagtgc cacttgtaga
    14521 tataaatata tattgatcgg gatctgtggg tctacctgca agaatattgt cagctatcac
    14581 actttcaatc aattcttttg cacgattggg taattgcact tcacgttgcg aataagtatt
    14641 ttttggagtt gtttttatag cattatccat tttcacagat gtgtaatcta aggtcccatt
    14701 aatggaaatt ttcccatcct catagtcctt catttgcaaa gctagcaatt ccccatatct
    14761 caaaccagtt aaatatagaa actcagctat tatgccgtgt agttttctgc gaggattgga
    14821 gtatagctgt ttcagtattt gatcaatttc ttctttctcc agatattttt tattcataga
    14881 aagccttctt ttttcttctt ccactttttt aggatggatt ttaactgcta gcgcagggtt
    14941 tctttgaatg tattttctat cgattgcata gtttagcata acagatagag ttgtttttgt
    15001 ttgttttgtg tagttcagtg agaggtcacc aaacgtatac atatcttcaa ttatcttatt
    15061 aatgagtgtc tcatcaatgt ttctaacgat cgtatcatcg cttatgtgct tagaaacatg
    15121 tttcatcatc attggaacct tcaaatagct agtacgttta acatgctgct tataatattc
    15181 ataccattct ttatacagct caccaaaagt gatatctgat ttattgtaat cttcgagtgc
    15241 ttctttaatt tttttatcta gaattttctg agcttttttc cacgcttgtg gtgaattact
    15301 tgtaagtgtt gtagattttt ttcgtgtttt ttctgtataa ggatctacat atctttcaat
    15361 aaacttgaat tggccatctt tggtctgttc aacccacact tttaacatct cctatcattt
    15421 gctataatag gcataacaaa tagacctata taggtttgtt ttctaaaagc acgctcttac
    15481 tctggacggt ggggcgtgtt ttttatttat tatacagttg ctttaaaaat agctgctttt
    15541 tcaaatgata ctaaaggaga gaagtggatt tctatttctc ccttttggtt taacgtgaaa
    15601 tgagcagtta catccatttg ttttcctgga gcaacagatc ccattgtatt ttcgttggca
    15661 tatgtctctg attttttgtc atctggtcca tatacttcca catctgtacc tacagggata
    15721 tctgaatcgc catcattttt tacggtataa gtaattttta ctacttgtgc aggttggttt
    15781 tcttcaaatt gatttctttc atcagttagt tctacactat ttagtgtata ttcagcatca
    15841 ccaactttta cagtgtcacc aatcttatag aaggtatcgc ttttttcttc tttagaagat
    15901 gaagcagagg ttgaagattt agttactttt tcgccaccat tatcacttgc tttattacta
    15961 tctgatccac catttaaagc agaaccaata attataatta aaattactgc taatacccaa
    16021 aaccatactt ttttgtaaaa aggtttacta actttataca tttttccgtc ttgacccata
    16081 actttttttg ccatttaaat attcctcact tcttgttata atatatttgc gatctcagaa
    16141 atgaggtatg agtccgtgtg ccagcacggg cttttttaat gttttgctga aatcggtttt
    16201 tttaataatt cttgatgtat tcatacatat tgccttgcgt aagaatattt tttcttaaaa
    16261 tagcattggc agacagcata acaagtgtat ctgagcttat tctgaataga ataatactcc
    16321 ataaattttt cgagattaaa ctgagattca tcagtcagtt cgttctcaat atagatattt
    16381 aataaaatta gaatagctat tttatctgct tctgtttcaa attttgagtg aaaagttgta
    16441 gaagtatcgt acaaaactga atattcaaaa tgagaagcgg tgaaatgcgc aagttcatga
    16501 gataaatgaa aggcttctgc agtttcgcca tatagatttt cattcaaaaa aatgattcta
    16561 ggcttcggat aatagaagcc aggctctttc atttccatgt aaactacttt caaattgtat
    16621 tcgctcaaca tttctttcaa tttcaaatac atacaaccca tcactccaac tattcatttt
    16681 cctctaaagc tttagcaatt gcaatcgctt tacgcattgt ctccttagat atttcttttc
    16741 catcaaaaga aaaaacagta tcgttttctg ataaatccac atgtttaggg gtttctcttt
    16801 cttctctacc tagaaggtag tctacagaga ctccaaagta atcagctaac cgagctaact
    16861 caatagaatt aggagtttgt cttttccaac ttcctaaata tccattcgaa tacccgaaat
    16921 tcatttcgag ctgtctaatg gataatcctt tttcttttgc taactccttt attatttcgt
    16981 aagtattcat tgataaatca acctttctga atgcttacaa aaaaagttta gataaataat
    17041 gtaaaaatag tt
//
LOCUS       DADOZM010000053        16935 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.054, whole genome shotgun sequence.
ACCESSION   DADOZM010000053 DADOZM010000000
VERSION     DADOZM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.054"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(12..1535)
                     /locus_tag="J8I47_001494"
     CDS             complement(12..1535)
                     /locus_tag="J8I47_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAZ6322934.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="J8I47_001495"
     CDS             1787..2968
                     /locus_tag="J8I47_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ6322935.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="J8I47_001496"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="J8I47_001496"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAZ6322936.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="J8I47_001497"
     CDS             complement(5053..7764)
                     /locus_tag="J8I47_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAZ6322937.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="J8I47_001498"
     CDS             complement(7774..8475)
                     /locus_tag="J8I47_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322938.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="J8I47_001499"
     CDS             8661..9260
                     /locus_tag="J8I47_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ6322939.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="J8I47_001500"
     CDS             9341..10354
                     /locus_tag="J8I47_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6322940.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="J8I47_001501"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="J8I47_001501"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAZ6322941.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="J8I47_001502"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="J8I47_001502"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAZ6322942.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="J8I47_001503"
     CDS             11802..13175
                     /locus_tag="J8I47_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6322943.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="J8I47_001504"
     CDS             13532..15676
                     /locus_tag="J8I47_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ6322944.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="J8I47_001505"
     CDS             complement(15728..16768)
                     /locus_tag="J8I47_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ6322945.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         4948 a         3409 c         3128 g         5450 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaa
//
LOCUS       DADOZM010000054        16841 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.055, whole genome shotgun sequence.
ACCESSION   DADOZM010000054 DADOZM010000000
VERSION     DADOZM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.055"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..455)
                     /locus_tag="J8I47_001506"
     CDS             complement(<1..455)
                     /locus_tag="J8I47_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ6322946.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="J8I47_001507"
     CDS             complement(560..1423)
                     /locus_tag="J8I47_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ6322947.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="J8I47_001508"
     CDS             complement(1619..3718)
                     /locus_tag="J8I47_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAZ6322948.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="J8I47_001509"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="J8I47_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAZ6322949.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="J8I47_001510"
     CDS             complement(5071..6912)
                     /locus_tag="J8I47_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAZ6322950.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="J8I47_001511"
     CDS             complement(7094..9487)
                     /locus_tag="J8I47_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ6322951.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="J8I47_001512"
     CDS             complement(9594..10454)
                     /locus_tag="J8I47_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAZ6322952.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="J8I47_001513"
     CDS             complement(10513..11034)
                     /locus_tag="J8I47_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAZ6322953.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="J8I47_001514"
     CDS             11354..12073
                     /locus_tag="J8I47_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6322954.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="J8I47_001515"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="J8I47_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAZ6322955.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="J8I47_001516"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="J8I47_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAZ6322956.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="J8I47_001517"
     CDS             16433..16789
                     /locus_tag="J8I47_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ6322957.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4623 a         3493 c         2954 g         5771 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgta
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaaa gaggctggga cagaagcgtt t
//
LOCUS       DADOZM010000055        16611 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.056, whole genome shotgun sequence.
ACCESSION   DADOZM010000055 DADOZM010000000
VERSION     DADOZM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.056"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            86..370
                     /locus_tag="J8I47_001518"
     CDS             86..370
                     /locus_tag="J8I47_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAZ6322958.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(651..1070)
                     /locus_tag="J8I47_001519"
     CDS             complement(651..1070)
                     /locus_tag="J8I47_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAZ6322959.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1072..1656)
                     /locus_tag="J8I47_001520"
     CDS             complement(1072..1656)
                     /locus_tag="J8I47_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAZ6322960.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1703..3178)
                     /locus_tag="J8I47_001521"
     CDS             complement(1703..3178)
                     /locus_tag="J8I47_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAZ6322961.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3359..3670
                     /locus_tag="J8I47_001522"
     CDS             3359..3670
                     /locus_tag="J8I47_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322962.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3785..6121)
                     /locus_tag="J8I47_001523"
     CDS             complement(3785..6121)
                     /locus_tag="J8I47_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6322963.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6263..6931
                     /locus_tag="J8I47_001524"
     CDS             6263..6931
                     /locus_tag="J8I47_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6322964.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6934..7977
                     /locus_tag="J8I47_001525"
     CDS             6934..7977
                     /locus_tag="J8I47_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6322965.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8214..9095)
                     /locus_tag="J8I47_001526"
     CDS             complement(8214..9095)
                     /locus_tag="J8I47_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6322966.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9254..9982
                     /locus_tag="J8I47_001527"
     CDS             9254..9982
                     /locus_tag="J8I47_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAZ6322967.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10138..10455
                     /locus_tag="J8I47_001528"
     CDS             10138..10455
                     /locus_tag="J8I47_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ6322968.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10448..11056
                     /locus_tag="J8I47_001529"
     CDS             10448..11056
                     /locus_tag="J8I47_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAZ6322969.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11072..11902
                     /locus_tag="J8I47_001530"
     CDS             11072..11902
                     /locus_tag="J8I47_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAZ6322970.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12040..12552
                     /locus_tag="J8I47_001531"
     CDS             12040..12552
                     /locus_tag="J8I47_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAZ6322971.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12578..13450
                     /locus_tag="J8I47_001532"
     CDS             12578..13450
                     /locus_tag="J8I47_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAZ6322972.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13626..13988
                     /locus_tag="J8I47_001533"
     CDS             13626..13988
                     /locus_tag="J8I47_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ6322973.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13997..14749
                     /locus_tag="J8I47_001534"
     CDS             13997..14749
                     /locus_tag="J8I47_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322974.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14763..16565
                     /locus_tag="J8I47_001535"
     CDS             14763..16565
                     /locus_tag="J8I47_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322975.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5195 a         2886 c         3407 g         5123 t
ORIGIN      
        1 aaaaaggaac tgcgacaaga cttttgtcac agttccttct tttttttgct atgctatact
       61 agatgtaaaa aataggagaa ataaaatgag ggaaatttat tcttttgaat caaaaatcta
      121 tgcttccgaa gtcgacaagg gcggggcata tattattttt ccgtatgata taagaaaaga
      181 attcggtgct ggaagagtta aagtccaagc aacttttgac aatatgccgt atgaaggcag
      241 tatcgtgaat atgggcgtga aaaatccaga tggttcagtc tgctacatcc ttggactacg
      301 aaaagatatt cgtaaaaaca tcggaaaaga aatcggtgat attgtagcag ttacagtgaa
      361 gcaaaaataa aagaggttgt gacaaaagac gtgtcacaac ctcttgataa acggtgttca
      421 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaacc catgtttaca
      481 aaatttttct tactactcga aacgaagcgg ctttatcaca acctcttgat aaacggcgtt
      541 caatcagctg tccctcggta ttagctcaaa aacggcaaaa tatgagaagc tatttttcca
      601 tttttttctc tcatactcag gtcataacgc tgatttcaca acctcttctt tcataatgtt
      661 ttttcaccat ctttataata aactggttct ttgatatgga aatctatttt ttccaaccaa
      721 tctaaaatct tgtaacctaa ctcttctgac atcggaaaag aggcgttgat ttgtgcattg
      781 gagatatggt caacatggat accggaagtg atcgtacaac ttcccggaac ggcagttttg
      841 atgatcacag ctattttttc ggctaagaca tcgtctttat ggaaccgacc atcgtggcta
      901 gggaaacgta cagtttgcat ctctgtatca cgagaaaatg tagtgactgt tccgatatgt
      961 ggtgtatctc cgcctgttaa agtgatcaat aagtctttac cgattacttt aacagcagct
     1021 tggatcgtga aacctagttt ggttacttca actatcgttt ctttttccat attaatcacc
     1081 ctgccaacgt tttgaatgct catttacaat tccaaattga tccaaaagtt tcattgtatg
     1141 gtgattaaca agatcttgga tcgtttgagg ctgattataa aaagcaggaa ttggtggtat
     1201 gatttgcaca cctagtcgtg ctaatttagt caagttttcc aagtgaatgg catttaatgg
     1261 cgtttcacga ggaacaatga ctaactttcg ttgctccttc attgttacat cagcagcacg
     1321 tgcaatcagg ttgtcgccta aaccaacaga gatacctgcg actgttttca tacttgccgg
     1381 tacgatgatc attccatcgg tcaaaaatga tccgctggcg acagcagcac ctaaatcttt
     1441 gtttgaataa tggtaatcaa acaggctttc gaattcagtt agtgataagt cggattccag
     1501 ctttaagttc tgttttgccc atggactgat gattccgtgt gtttctatca tcggatattc
     1561 ttttagtttc ttcactaggt tgatggcata aatggtgcct gaagcgccac ttacaccaat
     1621 cacgattttt ttcttccgtt gtgccaattg ttccattttc cgcactcctt tttatgatgt
     1681 caaaacagtt gcagatcatt cattatttca aatatttttc ccaatcttct acttcttgga
     1741 attttgcacg tacaaaatgt tctttcatat cgaaaggaac tgtaccgtca aagatagttt
     1801 ttgcactcat cccacggaaa cgaatagagc caggattata ttctggtaat tctgatgggt
     1861 ccaatggatg gttcctcatt cctgggatca cgatgatatc tttatctcct tggaaacgag
     1921 tgttgaaagt ccaaactaca tcgttcatgt cgaaaatatc aacatcttca tcgactaaaa
     1981 tgactgtctt caattctttg aaggctgata aagcaagaat agctgcttga cgctgaatac
     2041 cttcatccgc ttcgttttct ttacggattt gcatgatggt catcaattta ccaccacctg
     2101 caggaggatt gtagacattt actacttttc ctggaatagc tttatcgacc aaggcaagaa
     2161 tacttgcttc tgtaggaata ccggccatag atacgtgttc ttcactggga ccaatcgttg
     2221 tttgcatgat tggattgtct tttcggtgag taactgcttt gactttcact acttgtacag
     2281 ctggattagc atccccagta tagccaggaa attctggcat tgcttttcct gagttcgtgt
     2341 tgatatcttc ttgcattgtt tcatttggca tgatttctgc ttcaatgatg aattcagcac
     2401 gagcaatacc cacttcatcg actgctatac catcaacgat ttgaataggt tcgttacgca
     2461 aagctcctgc acaccatagt tcattgtagc caagtggagt ggtaggtggt tcgaaagtcg
     2521 tgccaatggc aattgctgga tctaaaccaa tattgatagt gataggcatt ggtttattca
     2581 aggcttcgaa ttgtttttgg aagtgaccaa tgtggcgtcc gccaggcatg atgtacatac
     2641 cgatcgtatc tttatcttct agaaccatac gatggatcgt tacatcgctc attgtttttt
     2701 ctggatctga tcctaagact acacccattg tgatgtatgg accagcatct tcaggagtat
     2761 ttgtaggtgc aggcaaaatg ttgcgaatat cgaagtctgc atcggtagct agatgaacga
     2821 cttcttgtgc tggagcgtct gctttgtcta ctttcacagg tttgattgga ttttctacag
     2881 cgtctttcaa gaaacgacca agtgttcgat aatcatggtg caaaatttca cctacacgtt
     2941 tacgactagc cattgttccg atgttcacac gtgtgccggg gaaacctttg atattgttga
     3001 aagtcatagc tggtccttct tgagtcggac gttttactgt accgccagca ccgatataac
     3061 ggtaaacacc agataattct gcttcaggat cgatttcaat atctgtttcg tgatattgac
     3121 ctggaagtgt ttttagttct tcgatgactt ttcttaaatc atacggttga tctgtcatga
     3181 gagattcctc cttagttgtc tttacctatc tatagtaaaa ggtgaaaaag aggaaaacaa
     3241 ttcaacttgt tcggtaaagt attctacaat ggaatggatg cgtttttttg ataatagaat
     3301 aaactatgat atacttatca ctaaaagaaa gcgcatactt agtgttggag gaaggaaagt
     3361 gaaaaaaatg aaggagtcat ttcctaaagc agtaaaagtg gaaaatatag cgaatatttt
     3421 gaaagttaca tttgaaaacg gagaagtaaa atacgtgaaa tctcattgga cagaagagat
     3481 aaccgatgcc ttgcaatttg gaaaaaaagg aagaggaaaa agaaaaaatc ttctcgcact
     3541 ttctcgtaat atgtggattg ggactgaagt aacgattgaa gcagatggaa cagtattcat
     3601 aaatggaaaa gacaggtata cgccagaaga gttgtggtac aaaggaaaga aaagtatacc
     3661 agaattataa aaaataatta aaaaaaacag cgcgaaacaa ggaacatatt acttcctcgt
     3721 ttcttgctgt tttctactat ttcagcgctc ttatgactca aatctaaatg tggttgtcac
     3781 aaccttattc tgcacgcaaa gcgatagcgg cgtctttttt cgctgccatt cgtgcaggaa
     3841 gatgaccgcc aagcatcgtc aatatagtag agacgatcac tagaataatc gcatggacag
     3901 gatttagttg agcaacattt tttagatccg tcatgttgta taagatgcta ttgataggga
     3961 acgtagctag ccatgcaata aacacaccta atatcccaga agaaatacct aaaatacacg
     4021 tttctgcgtc aaatacgcga gtgatgtctt ttttacgtgc acctaaagct tttagtacac
     4081 cgatttcttt tgtccgttca atcacagatg tataagtaat gatactgatc atgatcatac
     4141 ttgttactaa agagatccct gcaaaagcaa taaggacata agtgatagca tccatcaatc
     4201 cgccagtcag ttcagtcatc gttccagcta aatcagtata aatgatttta tcttcatctg
     4261 atttgccttt attgtaatca tctagataat ccaagatctt ttctttgtct tcaaaattgt
     4321 tcggataaat catgatactt gaaggcaagc tgtctccacc taaataacta agcaatgttt
     4381 gtttggcatt ttcgtcaact ttttcagtag ttaagacatt tacaccactg tctctttgag
     4441 ctttgacgat ttctgattct ttgttttcgt tgacgatttg agtcgttaat tgatcgctat
     4501 aagcgattcc tggagataag agattcattg tagaagaaga tttcacgcgt aggatccccg
     4561 agatcttcaa ttcatcagaa gcattctgat acatcgcatc ataatctgta ttagggataa
     4621 aattcccagt tggaagtttt gtatagtaag tattgttgtt tactaatttg aatgttgtac
     4681 caacgatatc atcaaaatca agtttttcgt cttctttcac atcaaatcca agattcttca
     4741 atgcgttgat attagtatta ttattgccgt ctacgatcag cacgacatct gttgctgagg
     4801 ctggataact tccggcaagt aaagaataat tgtcttttaa aaagttttct ttgctagtgt
     4861 caagctgtgt agggaaactt gacactccca cacctgtcat tgctgacatc gtactagata
     4921 aagaaagaga ttccgtatca ggattttgat tcgagaagct gacaggctgt actttgccat
     4981 ttacatcacg taaaagattg attcctgtag tacgggtaaa tccgatatta ttacttaagt
     5041 taggatcgat gtctgttacg taatcgacgt atgtttgatc aatcttattc gtgtgctgtg
     5101 ctttatcttc ttcgctgact tttgcagtaa tcgttttgct gtctggataa tccgctttgc
     5161 ttgcacccaa tccggcatcg tcacgagtct ggttagtcgt taccttagaa atagtaatcg
     5221 gaaatttcgc catcgtttca gcttgcgtgt tatcgatttg tttttggaat ccagaagaaa
     5281 gggacagaac gatcgcgatt ccgatgatcc caatactgga agcaaaggag gtcaagaaag
     5341 tacgtccttt ttttgttcgg atattgttga aggaaagctt taacgctgtc caaaaactca
     5401 tctttgttct gcgtaagtta aattgatcat cttttggtcg ttcgatgtga ggatgggaat
     5461 ctgttaagat cttaccatca gaaaattcaa tgatccgatc tgcgtattga tgggccagtt
     5521 caggattatg tgtgaccatg atcactaatt tttctttcga taactcttgg atcaattcca
     5581 tgatctggat acttgtttct gtatctaacg ctccggttgg ttcatcgcac aaaaggatat
     5641 ctgggtcatt ggctagtgct cgagcgatgg ccactcgctg catttgtcca ccagataact
     5701 gatttggctt tttatgcata tgatctgtca agccgactcg gtgcaatgca tcttctgccc
     5761 gttttcgttt ttcgtcttta gaaacaccac ttaatgtcat cccaagttca acgttttcaa
     5821 taatgcctag atgaccaatc aagttgtaac tttgaaaaat gaaaccgata gagttgtttc
     5881 ggtaggcatc ccagtcactg tccttaaagt ctttagtaga ctttccgttg attaccatat
     5941 caccggaatc ataattatct agtccaccaa tcacattcag catagtcgtt ttaccagaac
     6001 cactaggacc taaaatcgct acgaattctt tttgtctgaa agcgacagac acaccatcca
     6061 atgctttggt ggtggtttcg ccgactttat aatatttttt tatatctttt aattgaagca
     6121 taatcaccct tctcccgtat tgaaatattc ggtatactaa taagtatatc agaacaatga
     6181 tcgtagtata cgaaataaat gttaactgaa gatgaactac aagaaaaaat ctagaaattt
     6241 ttcaaaaaac agagggagat caatgaatcg tattttagta gtagaagatg atccgcaatt
     6301 acagttgctt taccgttctg tgttggaacg agcgggcttt tcagtcatca ccgcattgaa
     6361 tggagaggat gcgctgaaaa aactagaagc gacacgtata gaattaatca ttacagatat
     6421 catgatgccg aaaatggatg gctatgactt gctagactca ttacgtagca ccagatttga
     6481 cacacctgtt ctaatgatta cggcaaaggc cgattttgag gataagaaac gcgggttcaa
     6541 attaggagca gatgattata tgacaaaacc catcgatgtc aatgagatga tcctacgtgt
     6601 ggaagcattg cttcgacgtt caaagatcaa ttacagcagt agcttgataa tcggacaaac
     6661 aaaattagag ctagatgctt atacagtgtt acaagatcag cattcgttga ttctgcctca
     6721 aaaagaattt ctgcttctgt ataaattact gtcttatccc aacaagatct tcactcgcca
     6781 acagttgatg gatgatattt ggggattaga tacgaataca gaagaacgaa cgatcgatgt
     6841 acatatcaaa cgtttaagga cccgttttga aaagacggaa gattttcaaa tcatcacagt
     6901 acggggacta ggctataagg cggttattta aagatgaaaa aaaagctatt ttcacaatta
     6961 tggatctatt ttgctagtat tgtcttcatt tcgatcttag tgacactgct gtgttttctc
     7021 ggttttattt tcttattgtc tcgacattct atctcgcctg ctgaatcggc tggaaaacca
     7081 acgttatacc cgttattcgt ctttgcgggg ttcagtatgg tcgtgggtac aggaatatcg
     7141 atttttgttg ggcggcgtat cctgcatcca atcagtgcgc tagggacaaa tatgagtctg
     7201 gtagcaacgg gagatttttc gattcgaatg gatgaacagc agaaagtagc agaagtacag
     7261 caactgtata aagatttcaa cgttatggtc caagaactca atagtatcga gacactgagg
     7321 aatgactttg tgtccagtgt atcccatgaa ttcaaaacac cactagcaac aatccaagga
     7381 tatgttcaat tgttacaagc cccaaatctc tctgacgaag aacgccaaat atttttatat
     7441 cggattatcg aaagtatcac gcagctttca caattaacag agaacatctt aaaattgaac
     7501 aaactggaaa accagcgtat ccaactggaa aagaaagagt atcgattaga tgaacaaatc
     7561 agagaagtaa tcgttttttt gcagcctaaa tgggagaaag aacagctaga attggatatc
     7621 gaacttgctg cagtaaatta cacaggcaat gaagagtttt tgtaccaagt gtggctgaat
     7681 atcatggata acgccataaa gtacaatcaa atcaatggac agattcatat caaactattt
     7741 gagacagcca cagagattgg actggaagtg accgattcag gtgtaggaat gaatgaagag
     7801 accagagatc gaatgtttga aaaattttat caaggagata caagtcgtca aatttctggg
     7861 aatgggctag gactttcact ggttaaaaaa attctagaac tgcatgatgg acgtattgat
     7921 tatagcagta tcgaaggagt aggaacgaca gcaatgatac ggttaagtaa acaataggaa
     7981 aaagaataaa acccctaaaa attctattga aggtttttag gggtttttag tcgttttttt
     8041 gtgattaggg aaatgttctt acggttcttt tggcataaaa atcgctgctt tcttattgaa
     8101 ctacgagaaa tgttagaggt gtcattgaaa caaggacatg atgcaagaaa gagagcgaaa
     8161 gtcatgcgtc gctctctttt tttgggtgga tagatggaca gaaaatgtag ggcttagttt
     8221 tcttcttgat ttaaatactc ccaaaatgta tctgaagcca aagaatgctc accgacttta
     8281 cgagtaaatg caagataact ctctgttgtt ggagaaatag ggagaatcac agtattttct
     8341 tttaatagat tcttgactgt tttttccata acaatagcaa tccccatgtc atttgcaatc
     8401 atattgatga tcaagtcgat tcttgctcct ttataagaga tttttggttc aaatccagct
     8461 tcgcgacaca gatctagtac aggttgatac aagttcgtag tggggccaag gagcaaaaag
     8521 ttctcctttt tcagttcgct tagatccaat ttctttttac tagctaaagg atgattttta
     8581 ggtagaactg ctacaaaatc gtctgtttca gtaggcaacc actcaatcga acttggtggg
     8641 gcctggaaat ttctagcaaa gtggatcgtg tcactatgtt cattggcgtc attgtaagtt
     8701 aattcgacac tttccacttc tttcaactgg actgtaaatt caggatggcg tttcaaaaac
     8761 tcggtgattt tggaaaaacc tttataattg atcattgtcg gtatcgttaa gatatttaat
     8821 gaaagattgt tgtctttcgt ataggtttca agcacttctt gcatagcatg gtattgttct
     8881 acgacatttt ttacatatgg gagcaataag cttccagctt cggtcaattc gatttttcta
     8941 tgggcgcgtt cgaataattt gacgtctaat tctgtttcta acgaaagaat ctgtttagaa
     9001 atatttcctt gggtggtgaa ttgtcgatca gcagttttgg tgaaattcat tgtttcggct
     9061 aaatcaagaa aaacttcgaa tttatggata tccatcatat tcctcctact tattccaata
     9121 aagaatactt taacacaaaa aatgaattgg tgtaatcttc tctgtctgtt acaatctttc
     9181 ttgcagcgaa agcaataatt acagaaattt ttctgatttt tttcgcttcg tagaaaaaga
     9241 gaggagtttt ttgatgccaa ctggagtttt gatcaatgta ggttccgttt tgcttggcgg
     9301 actaattggc ggattagtag gaaataaatt aagtgaacat tttaaagcac agctgacgat
     9361 ggtttttggt gtgtgttcga tggggatggg aatctattcc attgcgccga tgaaaaatat
     9421 gccggctgtt atttttgctt tagtgatagg aacagcgatt ggtctgattg ttcatttagg
     9481 taatgggata aataaggggg ctgcgctgat gcaagtacct atttctaaaa tcttccctag
     9541 tgaaaagctt ggtatgaccc acgatgaatt tatcagcaca ttagtgacgg tcattgttct
     9601 attttgtgca agtggtacgg ggatctatgg aagtttagat tctgggatga caggagacag
     9661 tacgattttg atttctaaat ctgtccttga ttttttcaca gcagctattt ttgcgtgtaa
     9721 tttagggtat gtcgtttctg tgatagcaat tccccaattc attatttttt atattttgtt
     9781 cttattggca aaatttattt atccattgac tacaccagac atgatcttag attttaaagc
     9841 ttgcggcgga ttcttgatgg tcgctaccgg ttttagaatg atcaacgtga aaatgttccc
     9901 agtagctgac atgatacctg caatgatcgt tatcatgcca ctgagctgga tgtggacaaa
     9961 ttggattatg ccactgttat aataaaaatc aaagagaagc cattttttgc tgactaaaaa
    10021 ggatcaaatc ttttagtcag tttttttgta tttgacaaac tgattttgaa actatattat
    10081 atgttgtata atatagattg aaataaaata ttacacttga gaatagaagg tgacgtaatg
    10141 cacatccaga ttccaactgt tcttcttgat ggtactgtgc tagcagcatt aagcaaggaa
    10201 gatatgtatg ggtatgcctt aacaaaaatc gtacaagaaa gtctctctgt cagtgaatct
    10261 acactatatc cggttttgag aagattgaaa aaagaaggat atcttgagac ctatgatgaa
    10321 ccttttgaag gcagaaaccg tcgatattac tcgttgacac caatgggaag tcgtcattta
    10381 gaagaaatta ttgatgaatg gattgttttt cgagattctt tagaccggtt attggaggag
    10441 agcacaaatg aatgaatatt tgaatgaagt tgtggatcat cttcaagaaa ttcctgaaga
    10501 cgatcgtttt gatttgatcc agtattatga ggaatatttt ttagattctg gaaaaacatt
    10561 agatcagatc attgatgaat atggtacacc aaagcagttt gccttaaaat taaaaatcag
    10621 ttacttttca gaagaagaac aagcagaaaa cgagttagtg aaaaatccaa aaagacaaat
    10681 tcgcctgatt tggttaatca ttatcggttt atttgcttct cctgtcctca ttccactagc
    10741 actggggttt atattggtta ttgggggact attggttgct ttactagcgc tcatttttag
    10801 tatctatgtt ttatgtatca gtatcttagg tgttggccta ttcatgatca tctcaggaat
    10861 cagtgtgttt ggacaatcga ttctgagtgg attattcttt atcggtgtcg gtctgttcgc
    10921 cacaggaagc gcagtattta tttcgccact gttgctgaaa ggaacaaaag ggctttttta
    10981 tgtcatgatg aactttgtta aatggatggg acgacgtttt attacaaaaa gagatctgca
    11041 ttcagcgggc gtttgaagga ggacacataa attgagattg aaacattttt tgattaccgg
    11101 tattggtctg atgcttactg gtggcctcct agcaggtgca acttatacat taggcgctca
    11161 aaagagtctg acttgggata atggtcctcg gttgatcgat atggttactg agacaaaaaa
    11221 aatcaaggat gcaaacaaaa tcgtcatcca tgcagaaaat caaacagttt cggtcatacg
    11281 cggattggat tttgagataa aaacgactta tgataagaca gaaaagccag aaataaagga
    11341 gaaaaatggc gtgttgactg tgaatgcagt caaaaaatca acaggtgcaa tcattagttt
    11401 agaagacaca agtgcacaaa tcactattgt tgtccctgca gatcatgaat tatctgaatt
    11461 gacaatcgaa gggaataata gtgcaattga tatcaacgaa gtatcaatca aaaaagtgaa
    11521 tactaagaca catgatggct gggtatcgtt caatgaattt acaggaggaa caataactgc
    11581 gaaagatgaa ttaggctcac ttcgattaga tgaggccaat gtggataact tgatcgtcac
    11641 aggtgaaaac agcaacttcc atctagaaga cagtttgatc agcaaagaag gaaaagtcat
    11701 cctaaacggt ggcaatttat ctttgcatga gatggaaaac agtggctata ctatccaaac
    11761 gtctggtgaa tcttatattg aaaaagacta ccagccaata acaaatactt tgttagaaca
    11821 aggacagaaa aagctaaaga tcactgctaa agacacatcg atcaatatta cgacggatca
    11881 agaggacaat gaaacagatt gatcctgtac tcaactggat attttaagaa aaaacagcaa
    11941 agatccttcc aatctttgct gtttttcatc gcttaacgca cataactata gtaaaataag
    12001 aaagaaaata tctaaaaaga aaacagggtg aaagagtata tgaaggtaga agaaggtcaa
    12061 agagaaaagt taaaaacaga aatggaaaga ttacatactt atatcactca actaagccaa
    12121 accttctatg atccagacaa agaaaaagtg atggttaatt acccgaacaa ttcagaaggt
    12181 cgccaattag agcaagtgta ccatgaagtt ttcaaacatt tattgacggt gaagaaagag
    12241 ctggattatt attctttacc gattatcgat acaggaatat tgaagtatga tgggaagaag
    12301 gaacgattta tctttaaatc tgttcgggaa gatttgcctc tttcagcagg gatggatcta
    12361 gaggtcttgg tagaagacta ttttacagaa gaaaagcact gggttcgaac aaaattggat
    12421 tatttaccgc aggcagccgg agggacacag gcaaggggtt ggtacatcac tgaagacaaa
    12481 gagttagaac tagaaggtgt aatggcgcgt attcgaaaaa aaattaaaaa agtaacatta
    12541 gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa gagcatttta
    12601 tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg gattgttttt
    12661 cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta tttttctttg
    12721 gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc aagcaaaagt
    12781 aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt tccttacgat
    12841 acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt
    12901 aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag tctttaaagt
    12961 cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg tagaaatcca
    13021 aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat tcaatgagaa
    13081 tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa aagagctgca
    13141 agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta
    13201 tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc
    13261 tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag agcaaatcga
    13321 agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca ataatttact
    13381 tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg acatgaaaga
    13441 ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga ttattgtctt
    13501 gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga
    13561 ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag agagaagggg
    13621 aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta cttgcagggg
    13681 tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa attacgacag
    13741 atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac tatagatata
    13801 tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag ggacaattga
    13861 ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca
    13921 cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa aaactaatta
    13981 aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa aagtatacgg
    14041 aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg
    14101 cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt tgaatatttt
    14161 atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag atgtgaccac
    14221 attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta ttttccaaga
    14281 tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc tttctttaca
    14341 aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac gtttatccat
    14401 ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac aacgggtcgc
    14461 cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc ctacaggtgc
    14521 gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga atacaaaaga
    14581 tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt gccaacgtat
    14641 tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc aaagccgtga
    14701 ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg gggagattga
    14761 ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac tacttggtgt
    14821 tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca atggcaagca
    14881 acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt ttccgattcg
    14941 gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat tcctttttga
    15001 tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa
    15061 tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact ttagtgggtt
    15121 cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat cagttagatc
    15181 tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact gttttgtttt
    15241 ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga
    15301 ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat aaattcttgt
    15361 ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat atgatgaaac
    15421 acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc atattaggaa
    15481 cttatttcat tttccattct gtgatcattt tctttttatc attactaaag aaatcagaac
    15541 agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt cgtattcgtg
    15601 aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg ggcgctttga
    15661 cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat tctggatatg
    15721 atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa
    15781 cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat aatgcaagtg
    15841 aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg ccgaagatca
    15901 cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag gaacttcgaa
    15961 gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg
    16021 atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac ttccaagcaa
    16081 acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca tctttgcaaa
    16141 aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt ctattctctg
    16201 gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt gctagctgtt
    16261 tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa
    16321 agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta ggcgtgttat
    16381 tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa atgtttggtc
    16441 tattcatgca aaatccatac aaagatattt gggttccgtt tacgatcttc ttcgtattgt
    16501 atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg aaaaagagat
    16561 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata g
//
LOCUS       DADOZM010000056        16590 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.057, whole genome shotgun sequence.
ACCESSION   DADOZM010000056 DADOZM010000000
VERSION     DADOZM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.057"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(79..786)
                     /locus_tag="J8I47_001536"
     CDS             complement(79..786)
                     /locus_tag="J8I47_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAZ6322976.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="J8I47_001537"
     CDS             complement(865..1203)
                     /locus_tag="J8I47_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAZ6322977.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="J8I47_001538"
     CDS             complement(1314..2276)
                     /locus_tag="J8I47_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAZ6322978.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="J8I47_001539"
     CDS             complement(2291..3622)
                     /locus_tag="J8I47_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ6322979.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="J8I47_001540"
     CDS             complement(3630..4310)
                     /locus_tag="J8I47_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAZ6322980.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="J8I47_001541"
     CDS             complement(4529..5008)
                     /locus_tag="J8I47_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAZ6322981.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="J8I47_001542"
     CDS             complement(5135..6814)
                     /locus_tag="J8I47_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAZ6322982.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="J8I47_001543"
     CDS             7362..8864
                     /locus_tag="J8I47_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6322983.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="J8I47_001544"
     CDS             8877..9539
                     /locus_tag="J8I47_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6322984.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="J8I47_001545"
     CDS             complement(9640..9843)
                     /locus_tag="J8I47_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAZ6322985.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="J8I47_001546"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="J8I47_001546"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAZ6322986.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="J8I47_001547"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="J8I47_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAZ6322987.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="J8I47_001548"
     CDS             complement(13807..14421)
                     /locus_tag="J8I47_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6322988.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="J8I47_001549"
     CDS             complement(15027..15404)
                     /locus_tag="J8I47_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322989.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="J8I47_001550"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="J8I47_001550"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAZ6322990.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4807 a         3574 c         2833 g         5376 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DADOZM010000057        16165 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.058, whole genome shotgun sequence.
ACCESSION   DADOZM010000057 DADOZM010000000
VERSION     DADOZM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.058"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            79..1125
                     /locus_tag="J8I47_001551"
     CDS             79..1125
                     /locus_tag="J8I47_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ6322991.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="J8I47_001552"
     CDS             1474..2106
                     /locus_tag="J8I47_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAZ6322992.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="J8I47_001553"
     CDS             2694..4133
                     /locus_tag="J8I47_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6322993.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="J8I47_001554"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="J8I47_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="J8I47_001555"
     CDS             4895..5110
                     /locus_tag="J8I47_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6322994.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="J8I47_001556"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="J8I47_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="J8I47_001557"
     CDS             complement(5770..6681)
                     /locus_tag="J8I47_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6322995.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="J8I47_001558"
     CDS             complement(6700..7704)
                     /locus_tag="J8I47_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ6322996.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="J8I47_001559"
     CDS             complement(7701..9824)
                     /locus_tag="J8I47_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ6322997.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="J8I47_001560"
     CDS             complement(9829..12276)
                     /locus_tag="J8I47_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6322998.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="J8I47_001561"
     CDS             complement(12263..12652)
                     /locus_tag="J8I47_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6322999.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="J8I47_001562"
     CDS             complement(12712..13215)
                     /locus_tag="J8I47_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ6323000.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="J8I47_001563"
     CDS             complement(13228..13452)
                     /locus_tag="J8I47_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323001.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="J8I47_001564"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="J8I47_001564"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ6323002.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4791 a         3203 c         2884 g         5287 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag ctttt
//
LOCUS       DADOZM010000058        16011 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.059, whole genome shotgun sequence.
ACCESSION   DADOZM010000058 DADOZM010000000
VERSION     DADOZM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.059"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            226..894
                     /locus_tag="J8I47_001565"
     CDS             226..894
                     /locus_tag="J8I47_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAZ6323003.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="J8I47_001566"
     CDS             complement(1094..1480)
                     /locus_tag="J8I47_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323004.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="J8I47_001567"
     CDS             complement(1452..2834)
                     /locus_tag="J8I47_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAZ6323005.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="J8I47_001568"
     CDS             complement(2990..4177)
                     /locus_tag="J8I47_001568"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAZ6323006.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="J8I47_001569"
     CDS             complement(4184..5215)
                     /locus_tag="J8I47_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ6323007.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="J8I47_001570"
     CDS             complement(5231..6685)
                     /locus_tag="J8I47_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6323008.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="J8I47_001571"
     CDS             6860..7765
                     /locus_tag="J8I47_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ6323009.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="J8I47_001572"
     CDS             7856..8533
                     /locus_tag="J8I47_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ6323010.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="J8I47_001573"
     CDS             8814..9710
                     /locus_tag="J8I47_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6323011.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="J8I47_001574"
     CDS             complement(9765..11135)
                     /locus_tag="J8I47_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323012.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="J8I47_001575"
     CDS             11373..12119
                     /locus_tag="J8I47_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323013.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="J8I47_001576"
     CDS             12116..12955
                     /locus_tag="J8I47_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ6323014.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="J8I47_001577"
     CDS             12956..13906
                     /locus_tag="J8I47_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323015.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="J8I47_001578"
     CDS             14047..14799
                     /locus_tag="J8I47_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323016.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="J8I47_001579"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="J8I47_001579"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAZ6323017.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5195 a         3004 c         3224 g         4588 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg t
//
LOCUS       DADOZM010000059        15955 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.060, whole genome shotgun sequence.
ACCESSION   DADOZM010000059 DADOZM010000000
VERSION     DADOZM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.060"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(21..905)
                     /gene="rsmA"
                     /locus_tag="J8I47_001580"
     CDS             complement(21..905)
                     /gene="rsmA"
                     /locus_tag="J8I47_001580"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAZ6323018.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="J8I47_001581"
     CDS             complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="J8I47_001581"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAZ6323019.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1460..2236)
                     /locus_tag="J8I47_001582"
     CDS             complement(1460..2236)
                     /locus_tag="J8I47_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAZ6323020.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2391..3713)
                     /locus_tag="J8I47_001583"
     CDS             complement(2391..3713)
                     /locus_tag="J8I47_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323021.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3888..4475
                     /locus_tag="J8I47_001584"
     CDS             3888..4475
                     /locus_tag="J8I47_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323022.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4954..6963)
                     /gene="metG"
                     /locus_tag="J8I47_001585"
     CDS             complement(4954..6963)
                     /gene="metG"
                     /locus_tag="J8I47_001585"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAZ6323023.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7231..7509
                     /locus_tag="J8I47_001586"
     CDS             7231..7509
                     /locus_tag="J8I47_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323024.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7606..8427
                     /locus_tag="J8I47_001587"
     CDS             7606..8427
                     /locus_tag="J8I47_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAZ6323025.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8712..9722)
                     /locus_tag="J8I47_001588"
     CDS             complement(8712..9722)
                     /locus_tag="J8I47_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6323026.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQAGNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9734..10687)
                     /locus_tag="J8I47_001589"
     CDS             complement(9734..10687)
                     /locus_tag="J8I47_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6323027.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10677..11747)
                     /locus_tag="J8I47_001590"
     CDS             complement(10677..11747)
                     /locus_tag="J8I47_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323028.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11753..12796)
                     /locus_tag="J8I47_001591"
     CDS             complement(11753..12796)
                     /locus_tag="J8I47_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323029.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12797..13738)
                     /locus_tag="J8I47_001592"
     CDS             complement(12797..13738)
                     /locus_tag="J8I47_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323030.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13822..14169
                     /locus_tag="J8I47_001593"
     CDS             13822..14169
                     /locus_tag="J8I47_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAZ6323031.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14239..15900)
                     /locus_tag="J8I47_001594"
     CDS             complement(14239..15900)
                     /locus_tag="J8I47_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6323032.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4489 a         3298 c         2866 g         5302 t
ORIGIN      
        1 ttctgtcaca acctcttttt ttactgcata ttttctgaca tcgcattgct taatgcggcg
       61 aattcttgta atgacaaagt ctccccacga cgttttggat cgattccagc tgtttccaag
      121 ctttttgcta accatgcttt tgtctggtca tctttgccat acgaatgctg caagttattc
      181 caaagcgttt ttcttctttg ctgaaatgcc gcttttgtca atcggaaaaa tgctttttcg
      241 tctgtcactt cgactgccgg tatatctctt cttgtcagtt tcaaaatcgc tgaatctaca
      301 tttggctgag ggacaaatac tgtttttggt acgatgaatg ctaaactggc ttccatataa
      361 tattgaacag caatcgataa tgaaccataa gctttcgttc ctggttcagc agagatacga
      421 tcagctactt ctttttgcat catgacgact agttcatcca caaccaaatc tgattcaaga
      481 aaatgcatca tgatcggtgt cgtaatatag taaggtaaat tcgctacgac ttttaacgga
      541 agctcctcat gaaatgtttc tctaacagct gttgacaaat cggtctttaa aatatcttga
      601 tgtacaatag tcacattgtc atagggttgc atcgtatcag ccaatacagg gatcaaacga
      661 tcatcgatct caaatgctac gacttgtttt gcatgcttgg ctaattgttc cgtcaatgca
      721 ccgattcctg gtccaacttc gatcacgttc gtatgttcat ctatttgcgc agtttctacg
      781 attttcctta aaatatttgg ttcagtcaga aaattttgtc ctaaactttt tttaaatgaa
      841 aaaccatgtt tcaataaaat ctcttttgtt ctagagggtg tcgcaatttc acgatattca
      901 ctcatgttgt tcctccacta ttttcattgc ttgggcgagt tctttcggcg taatacgaaa
      961 catcttcaaa cgtttttcca attgtttgcc gttcgtgtaa ccgatgcgca actcgtcacc
     1021 tagtttttct cgtttttctt ttgcttgtgc cccagctatt aaaccatagt ctaaaaggtc
     1081 ttgtctagtg atttcagaaa cagccgtttg ggaaatgtct ggcgtcacga cctttttcaa
     1141 tgcttctaaa atagcagcat cacttgcatg ctcgacacca aggcttccct ttttctttgg
     1201 tttagcttgt gtgcgcggca aaaaagcatg ttttgcctga ggaacagctt ccataatgat
     1261 ttttcttatt ttttctcctg aaaaatcagg atcggtaaaa acgatcactc cacgagtctc
     1321 ttgggcatgt tcaatttgca tcaggatctc ttcattgatt gcagacccta ttgtttctat
     1381 cgtatctgca tcaactactt cccgcagcct tcttgtatcg tcttttcctt caacgactat
     1441 aatctcttgg atcttcatct tatccatgtt cgcttagacc aaataatcgg tgcgcatttg
     1501 tccatgtttg ggcagccact tcttctgtcg gaagattccg aagttcagca atcttttcta
     1561 ctgtataacg agtatagcct ggttcatttc gctttcctct aaaaggaact ggtgccagat
     1621 aaggggcatc tgtttccaca agtaatcgtt cgattggtac atttgctgca gcctcttgga
     1681 cgtctaatgc ttttttgaaa gtgacgacac cgctaaatga aatatgcatc cctaagtcaa
     1741 gaaatcgttt tgcccattca taatcaccac tgaaactatg catgattcca ccgatatctc
     1801 gaacatcttc ttcttttagg atttgatacg tgtcttcaag tgcttctcta gtatgaatac
     1861 tgattggtag gttcatttct tttgcgatag caatctgtct gcgaaatact ttatcttgga
     1921 cttctttcgg gtcttccatc caatgataat ccaagccaat ttcccctaat gctacaacct
     1981 ttggtaaagt caattgttct tgtaatttat tttcgatttc tttcgtataa ctgccagcct
     2041 ctgtcggatg ccagccgata atactgtata tatttggata gtcatggctt aattgcaaag
     2101 acttttcaat cgttggcgta tcgaagccca cgaccgccat tttcgttacc cccatttctt
     2161 gtgctcgctg aatcgtttct ggaatatctt cattgaactg ttcagcattt aaatgggtat
     2221 gagaatcaaa tatcatgttt atctctcctt ttcattctta ctttagcaca taagcactac
     2281 ttgcagaagt agcaaaaatc aaaatagaag aacttgtttt attgaaatag aaaaagcacg
     2341 aagatctaga ggacctagtt cgtacttttt ctgtctgttg ttgaaatatt ttactcttct
     2401 gagcgttttt tctttttttg ataatagact gggataccta tcaaggtaac acctataccg
     2461 atcaatgcta atttcgtttg cgtcatcatc gtcataccta aaatgaaaat agcaccgaga
     2521 atcgcaataa tcggaacgat tggataaagt ggtactttat atggacgtgg caattccggc
     2581 gctttttttc ttaaaataaa gacaccgata aaaatcagta aactgaatag ccacatcaca
     2641 aaaatcaaca tgtctgttaa aaagtcgaaa gaaccaaggc tcatcataat acaagctact
     2701 gctaactgga aaaccgcagg aacataagga accgtaaact tctttgaaag ctttgtcagc
     2761 tgtttactga aaggtagctc gtcttctaaa gccatcgcat aaggcacgcg gatcccagtt
     2821 agtgtataac cgttcaatgc accgtatacc gagatcaaga tcccaatagt gaccagcttt
     2881 ccaccgattc ctccaaaaat gacgtctgat gcttcagaag ctgcattcaa attaccggca
     2941 agatgatcaa tcggcaaagt tttcaaaaaa acaaaattga tcaatagata aacgactgtt
     3001 acaaaactca aaccaagaat aattgcttta ggcaaatctt tttccggccg tttcatttcc
     3061 cctgccattg caccgacacc taaccagccg tcataagcaa acaacgttgc taacaacgca
     3121 ctgctcaatc cttcgacaaa agtaacatct tttccagctt cgattggaaa taactggaca
     3181 gttccttgtc ctggggtcaa taatccccat atgacaatca cagcaattgg tattaattta
     3241 acaatcaacg tcactgattg tacagaagca ccgacttttg tccctaacaa attcaaccct
     3301 gtcacagaaa cagcggtgat cactgcaatc aacaaaagat aatcggtaga taattgtaat
     3361 aagttcgtca attgtgtcgc aaagataatc gataatgcag caatattagc aggaaagtaa
     3421 atcaagcttt gcgcccatcc taataaaaag ctgggcaatt ttccatacgc agcttcaata
     3481 tattttacag ctccaccagt ttccgggatt gctgtagcta attcagccac tgtcaatcca
     3541 gcacagatcg ttaagaaacc acccaaaagc caagctagca atgtcaaact tgtagactga
     3601 gtactagcag taacagcagc agttttgaag aagacgcctg caccgatcac ggttcccata
     3661 acggtagcta atgcgccaaa cagattgatt tctcgtttta gtttgccttt cattcccatc
     3721 ctctttcttc tttgaaattt ggtataattt tcagtataac tgagaatatt ttaatttacc
     3781 attctttttt ctccactttc agtgaaaaac tccctcttaa gacggatgta tttctttttt
     3841 atttttcgta tactaaacct accttttcca cgaaggagga acagtttatg gattttaagc
     3901 gttcttatcc gaaattagca gattatttat ccaaccgggt cgatttttcc cgtaaagaac
     3961 aacagcagtc ccattggcat tggttcacat taattttatt tggattaatg actgttgcaa
     4021 cgttatcaca aaaactaata ttgctgactt tgttcatcgc aatcgttgca ttgatcaaag
     4081 gtcctgtgat gatcttgtgg gggatgttgt attccttttt ggttgggctt tttccgccta
     4141 tcggtatttt gctttcagcg attttctttt tattgaatat cggaacattt acaaagaact
     4201 ggcggatgac cttagtcggg atctattttt atttctatcc ttttggagtg atggcactta
     4261 gtgaagtcat gcattgggat aatcattggt ttatagctgg ctccttgctt ttagggttga
     4321 tcttgttaca cgtgatgttg acaaagctct atcaacatta tggtatcgga cgtacgattt
     4381 tctggtatgt cttttcgatc ccatttgcac tgcttactgc tctcttacca agccgtttga
     4441 agacaaaaat aaaaggttac cataaaatca agtgatagga tttttacaaa ataaagaaaa
     4501 caatcatcga agtcaaagcc gatgattgtt tttttatcta tattttcaat cacacatata
     4561 agagcacaaa ctaattgaac ataaagtgat agaacttata attaacacat caacgctact
     4621 gttagatata aatatagatg taataaagtc attgcattca aactttatga atgtaaggac
     4681 aataaaaaag agtaccagat gaatcaatta aaatctgccc gaccctttat cattatgctt
     4741 tttttctgtt tttacgattc ctattagtat tattctaaat taacaaaaat cagcgaagct
     4801 cgtacttctt tttctaggat ttattttgga tagttctctg tttctgtatt atatgattgg
     4861 cttcaagtaa taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta
     4921 tgacacaact agtgtcatac cccctttgaa tttctatgcg atttctgagc catttggcat
     4981 ggattttggt gcttcgatta cttgtaaaga gccatctggt gcttctgcag aaaggatcat
     5041 tccttgactg atttgtccac gcatctttcg tggtttcaga tttgctacaa tgacgacttt
     5101 tttcccgatc aattcgcttg gatcaggata aaattcagcg attcctgaca agatttgacg
     5161 gtcttggctg tctccagcat ctagtcggaa ttgcagcaac ttatctgccc cttctacttt
     5221 ttgacagttg atgacttctg ctacttttag ttctactttt tcaaatacat caaatttgat
     5281 ttgtttttct ttcgttgaaa caagttctgt ttcttctgga tcccatttga ctgtatcttc
     5341 gtttgtttga gtaccttcag acattttttc ttgaataaaa gcaacttctt cttccatatc
     5401 taaacgtggg aagattggtg taccttttgc aactactttt gttcctgaag gaaattcgcc
     5461 aaagtgtaat ccttcaagat tcattgtttc agagtccaat cctagttgat tgaagatctt
     5521 ctctggcgtt tctgtcatga ttggctgaag taatatcgct gcgatacgca aagattcagc
     5581 taaatggatc atgacactat tcaattcagc tttcttttct tcgtcttttg ctaataccca
     5641 tggttctgtt tcatcaatgt atttgtttgc acgagaaatc agcacccaga tttcagccaa
     5701 tgctgtattg aattccattt tttccattgc atcatggtaa cgtccgacaa cgttagcagc
     5761 cgtcgtagac agttcactat caaatggagt gacaagtgag gcatagtcag gaacgagtcc
     5821 atcacaatat ttgttgatca tagcaatcgt tcgattcaat aagttcccta gatcatttgc
     5881 cagatcataa ttgacacggg aaacaaaatc ttctggcgta aagacaccat ctgacccaaa
     5941 cggaacagca cgaagcaaat aataacggag tgcatctaaa ccataacgtt ccacaagcat
     6001 ttcaggataa acgacatttc cttttgattt agacattttt ccgtctttca tcaatagcca
     6061 gccatgccca aatacttttt taggtagagg caaatcaagg gccatcagca tgattggcca
     6121 atagatcgta tgaaagcgaa cgatttcttt accgaccatc tgaacatctg caggccagta
     6181 tttttcgaat agactttcat cttcggagcc atatcctagt gcagtaatat aattggataa
     6241 tgcatcgatc catacataca ccacatgttt cggatcactc ttgactggta ctccccaagt
     6301 aaaagttgtt cgtgaaactg caagatcctc taatcctggt ttgataaagt tgttgatcat
     6361 ttcgttttta cgagattctg gctggatgaa ttcgggatgt tcttcatagt attccaatag
     6421 acggtctgcg tatttgctca tgcggaagaa gtatgattct tcttttacca attctacttc
     6481 gtgtccactt ggcgctttac caccgatcac gttaccttcg tcatctttgt acacttctgc
     6541 aagttgtgtt tctgtaaaga attcttcgtc agaaacagag taccagcctt catattcacc
     6601 taagtaaata tctccctgtt ctaataaacg atcaaatatt ttttgaacag ctgctttatg
     6661 gtaatcatct gtcgtgcgaa tgaatttatc ataactgata tcaagcgttt tccataattt
     6721 cttgacatca gccgccattt tatctacata ttcttgcggt tttacaccta gttcttctgc
     6781 ttttgtttcg attttttgac catgttcatc cactccagtg agataaaaca catcaaagcc
     6841 catcaaccgt ttataacgag caattgcgtc acatgcaatc gtagtatacg agttgccgat
     6901 atgaagtttg ccgcttggat aatagattgg cgtagtgata taaaaagttt ccttttctgc
     6961 cacggggctc ttcctccttt atcgcatgat gcgaattcgt ttccttgcta gtataccata
     7021 aaaaaagcat gctgttagct ttttttcaaa atgaaaaaga ctggataaac gacttctatc
     7081 tacgttcaat ctatccttta aaaggatgaa taggttgaat aaatgcgggt ttttgttttg
     7141 tttcatatct actaaaaaac ggctttttat ttctcccctc acacgtggta taataaagac
     7201 tatctatctt agtaagggac tggaacaatc atgcagaaga aacatctttt ttttacactt
     7261 agtattgctt ttctttcatt agcacattta atattcagct atttttatat tcgtatgtat
     7321 ggttatttta acctccatgg gcatttgaac agttttatga cagcagcttg gattttgcgt
     7381 tttatcattg acgtatatat cgttatttgt ggcttctttg ccattcgtga agagcggtat
     7441 aaagtattgc cgttttatct gcttttcttt ttattcaatc tcatacttcc atttattttt
     7501 catatttaaa tgataaccta gatttttcaa gtaaatcctt ggtttttttt cagagtctgc
     7561 tacaataagg taaaaagtac ttttactgtc aatggaggtg gtcagatgaa ccgttttgct
     7621 tctaaagaag aagaaaaatt atattatgaa cataaagcaa gtgaagaaga atttcttaac
     7681 tggtatcaaa aacaggaacg tccagaatat gacaagccct ctttgactgt cgatatcgta
     7741 ctgatgtgct ataacaaaga agaagatcag ctgaaagttt tgttgatcca aagaaaaggg
     7801 catccttatc gaaattcatg ggcattgcct ggtggctttg tccaaaagga tgagtcaact
     7861 ggagaaagcg tcttaagaga aacaaaggaa gaaactggtg tggttatttc aaaagaaaat
     7921 attgagcaac tgcatacatt cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca
     7981 gtcagctact tagcctttat tggagaagaa ccgctaatcg ccggagatga tgccaaggaa
     8041 gtacgctggt tcacactaga acgtcatagt aacaagatcc atttatctag cggagaagtg
     8101 gctatctcgc ttgatttagt cactggagag tcttctggca aagatacttt ggcttttgac
     8161 catagtcaaa tcatcttgaa agcattcaac cgtgtagtca ataaaatgga acacgagccg
     8221 caagttctac aagttttagg taaagatttc acgattacgg aagctcgaaa agtatttgct
     8281 aaattcttag ggatcgatta taaaacgatc gatcattcca attttaagaa agctatgctc
     8341 caatatttcg atgagatcgg tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat
     8401 cagctcaagc cggggcacca tgaataaggt tgtgagcaaa agattcagac ctaatcattc
     8461 aagaaaaaac gcaaaaacaa ctctttgtga gttacgtttt ttgtttcaga attttgatgt
     8521 attcgtaaac tttctgaata aaaaattaac aatagcagaa aaagctcgta cgaacttttt
     8581 ctgctattgt tttattggtt tgtcataatt agtgagcttt tcttcaaaag aaaaattcct
     8641 agaaacaaaa gtcattaaga ctttttatta acaaggctaa ccctaacgct cctatcagtt
     8701 tcattcattt tttatccctt gataaccgaa tgtctgccca ttttttctac tacttggctc
     8761 tttcttacgg aattgctttc ttctgctccg tcggtattga tcaaaagaaa ttttttatta
     8821 taccattggg acttataacg caattttgtt attaatttat gccagtcgtg aatagaaaaa
     8881 tcagcatgtg cctcttcttt tggaaaagat tctacaaata agtagtcata ttgtttgacc
     8941 aatttcccag ataattgttc aagataatct tctcgctggt tcttttgttt cagataaaga
     9001 tccattactt ttctcttttg tttctgataa ttgcctgcct gatctaacct gatctttcta
     9061 tggtgagcgg agcggacttt cacattcaat ttccgctgtg catgttgcag cttttcttct
     9121 gtctggtgaa attttggtaa agttacttct agaggtcgag atgtttggat caatgtcttc
     9181 ggatgatagg cgatcccgat ccatttagct aaagattctt tttttactgg cgaaacatca
     9241 caaagtagtg cgacataaaa ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc
     9301 gatccttcaa ctggccgatg ctgatttatc ttgattagtt ctttttgttt tggtaacttt
     9361 aagtagccat tttccagata gatcgtacgg gtttggttgt tggtcgtata agattgccac
     9421 atactttttt tcgttttaag ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa
     9481 gcccgatcca aattaagctg tgcatttgct aaagcaagac tgtctacttc ttttaagaat
     9541 gggtattctt tttttaatgt agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc
     9601 caacttttct gccgcttttt cagcaaatgg ttataggtaa atcgagcaca tccaaaagta
     9661 cgaatcaatt gctgtttttg ttcttctgtc ggatacatac ggaatttaaa agcttttagc
     9721 atctatgtcc ctcctagtta gcaaccagaa gagattcctt ttgtcgtttt agtttttttg
     9781 cgtaatatgg gacttcctgc tcagttgcat agacaaaatg ttctggtgcg atttcacgaa
     9841 gttgacgtac ttgtccgtca tctggttcag gttgatattt gattcttctg cgttttctct
     9901 catggtctgg atctggtaga ggaatagcag ataacaaact ttctgtataa ggatgaacac
     9961 cgtgattata gatttcatca cttgttccca tctctaataa caagccgtta tgcattacac
    10021 cgattcggtc actaatatgt ttaaccatcg acaaatcatg tgcaataaat agataggtca
    10081 attgatgttc tttttggaga tcttgcaaca aattgaccac ttgggcttgg atcgacacat
    10141 ctagtgcaga aattggttca tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg
    10201 caataccaat acgctggcgt tgcccacctg aaaattcatg aggataacga gtaccgtggc
    10261 ttggattcaa tccaacagtt ctcaatagat catcgacttt ttttgcacgc tcttcagatg
    10321 atttagctag tccattaaca tcaattcctt cggcaatgat atctctgact ttcatacgag
    10381 gattcaacga agcgtatgga tcttgaaaga tcatctgaac atcatgccgg aatttcgtca
    10441 aacctttttt atctttgatc ttcgttatat cttgaccgtc gaataatatc tcaccatcag
    10501 taggttcatt caagcgaatg atcgtacggc cagtagtgga ttttccgctt ccagattctc
    10561 caactaaacc gaatgtttct ccttcataaa tgtgaaagct gatgtcattt acagcatgta
    10621 cttcattctt ttttcctaca ttgaaatact gtttcaatcc tttaacttca agtagtttat
    10681 ttggcatcag caattcctcc ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc
    10741 cagcgacgtt ggatttcttc tggcggtgtc actttgggtg cctgcggagc aagtagccaa
    10801 gttgctgcct tgtgtgtttt tgatagttca aaaaatggtg gttcttgttc tgcatcgatt
    10861 ttcatcgcga attcatttct tggatagaat gcgtctcctt ttggcgggtt caataaatcc
    10921 ggtggagaac caggaatagc atacaatcga tcgttcgcac tatctaatgt cggcattgaa
    10981 ccaagcagtc cccaagtata gggatgttgc gggttgtaga aaatttcttc cgatgttcca
    11041 acttcaacga ttcttcctcc atacatgaca gcgacacgat cagcgacatt cgctactaca
    11101 ccaaggtcat gagtgataaa gataattgat gtatcgattt ttgtctgaat ttctttcagc
    11161 agctccaaaa tctgtgcttg aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc
    11221 aggatttccg gataacatac cagtgcaatc gcgatcacga tccgttgacg ctgacctcct
    11281 gagaactgat gcggataact gttgatacgt ttctcagctt cgggtattcc aactaattta
    11341 agcaattcta atgcagcttt ttgtccctct ttttttgaaa ctttattatg ctttctaagg
    11401 gactcagcga cttgcttccc aatgggcatc gtcggatcta aagaggtcat tggatcttgg
    11461 aaaatcatcg ctatttcttt accacggatt ttttgcatct ctctttctga ttttccaacg
    11521 atatcgttac ctttaaagag gatttgacca gcatctatat tggcgttgct tgacagcaag
    11581 cgcatgatgc ttctggtcgt cactgatttt ccacttccag attccccaac gatcgccaat
    11641 gtttctcctt tattcaaatg aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac
    11701 gtatcaaaag agatttctaa gtctttcact tctaaaattt tcgtcatcgt cttcactctt
    11761 tcattttagg atcaaaggca tcacgcaatc catcagcaag taagttaaag ccaatcatga
    11821 taaccgataa agttactgcc ggaatccata atagataagg cagatacagg aatgtcttgt
    11881 acccttcgct caacatcgtt cctaatgaag ctgacggagg cgtaagacct aaaccgataa
    11941 agcttaaaaa ggcttcgaag aaaatagcag atggaatact aaacatcatt tgaataataa
    12001 tgacactaga gatattcggc aaaatatgtt tgaatgcaat tttcattctt gattcaccta
    12061 atgtcaatcc agctaaaaca tattcttgat cttttaattt cattgtttgt gcacgaacga
    12121 tacgtgccat tggtatccag tttgtgatgg ccatagcagc cacgattgaa aaaattcctg
    12181 gttcaaaaac gaccaacatc aaaatcatga caacgaggtt tggaattcct gataagattt
    12241 ccaaaaaacg ttgcatcacg ttatcgacac gtccgcctaa caaaccagaa accaacccgt
    12301 aagctacacc aatcgttaca tctagtaatg cagcaataaa tgcgatcagc aacgagattc
    12361 tcattcccat aaataagcgg cttaatacat cacgtcccaa accgtctgtt cctaaaaaga
    12421 aatttacgtt tgaagggacg tttgcttgag catatttatc aacaagttca ccaccaacca
    12481 ttgcttttcc attcaatcca tcgatattga ttcccggaat acgaggaggc aaattgatat
    12541 aagccacgtt ttgggcagtt ggatcatgag gagaaacaaa gattgtgatc acagagatca
    12601 aaatgatgac caatagtaaa accattgaaa taacggcagc tttgtttttc tttaaacggc
    12661 gccatgaatc ttggataaaa ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg
    12721 tatttttttg taatggaaga aattcatccg cagggatatc tgcaactgtt tcataacgct
    12781 ttggtatcat ttccatttat cctcggctcc cttctgaaac acgaattctt ggatcaacta
    12841 gaccgtacaa tagatccacg atcaaaataa caaaaatcag cataaatgag taaaggatcg
    12901 ttacagccat gatcgtagga taatcatttg ttgtgattga cttaacgaat tgttccccga
    12961 taccaggaat agcaaagata ttttctacca ccagtgatcc ggtcatcaat gctacagcta
    13021 acggtcctaa taaagtcatc aatggaatca aactatttcg aagaccatgt ttaaaggcga
    13081 tttcccaacg gctcaatcct tttgcttttg ctaattccac ataatcacta tgcagtactt
    13141 ccaccatttc cgtacggata aaccgagcag agtcagcaag cggcgacatg gccaacgcaa
    13201 ttgttggtaa gattgtgtac acaaagccat cccattttgc gattggcaaa acttgtaatt
    13261 tgattgcaaa gatatattgt aataaaacag caaatacaaa gtttggaatg gaacgaccta
    13321 atatcgctac gagtgtagaa gatgtatctg cccatgtatt ttgcttcatg gcagagatcg
    13381 ttcctaaaat cgtccctagg actgttccaa agataattgc ctgtaaccct aactgcaagg
    13441 atggtccgat tcttccagct aacaaatgtg cgactggctg gtttttaaat tggaaggaaa
    13501 ttccaaaatc tccttgaagc aaattagaaa gataaatccc atattgtacg ataactggtt
    13561 tatccaaacc gacttgttta tttagcatct cgattgtttc aggactcaaa cgttcctgat
    13621 tcgtatatgg cgtgcccggc agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc
    13681 aaagcgtgat gatcatgaaa aagacacgtt ttaatacata tttgatataa ctattcacta
    13741 atgtttcccc ctagcttttg acttctgaca gatgtttcgc tacattatta gataatgaac
    13801 caaaaataaa ggagcaatca aatgaaaaaa aaatctatcc cctacgcagt agcttttcta
    13861 ctgattctgg ttattttaat aaaaaatctc atcaaccatt cctttacact cattcaatta
    13921 tctaatgatc tattcttatg gtcattgccg tttctgatta tcggcggttt tttatgggtc
    13981 ttttcttcgg gtttcttcga tcattttcag cgatcggtcc atcttgcacg aacaaggaac
    14041 cgaaagaaaa aaccagaatt ttcctcgctc tcgtctgcca gctacggtat gtacagcttt
    14101 tggctgatca ttgccggaat actaattgct ctttctgcta ttttcatgct tttttctttg
    14161 ttaggataag tgaatatgag aacgaggttg cgacatttgc cgcagcctcg ttttttctca
    14221 tgctcacggt attaacgatt attcttcaac gtatgtccat ttgtaatccc atgaagcgcc
    14281 tgccgcatgg tgaacgatac ctttgatttt ttcattcact aggtgacctt ctgctttttg
    14341 gtacaccgga acgacaccca tatcatcaga gatgatcttg attgcttttt ggaaatcatc
    14401 ccaacgggct tgttcatcgc cagcgtctgt tgatgaagct gctttaactg cttcgtcata
    14461 ttctgcattg ctccagcgac cacggttgta tgaattacct gtgacaaaca aatcagtaaa
    14521 gctgcttggg tctgcatagt cagcagccca tccgcctagt actgcgtcaa aatcacccga
    14581 agttgagcgg tcaagacgta cagagaatgg aacaggtgtt ggtttgactg ttacgccatc
    14641 caagttttct tggattgagt tttgaacata ttcgattact tttttcgatg aatcatcatc
    14701 agaagccatc aaatcaaatt ctaaagaatc aacgcccaat tcttttttcg ctttttccca
    14761 atattctttt gctttatctt tgtcataaga aactaatttt ccagcttctt ttgcaaagtc
    14821 ttcgttcgtt gttggacttt ttaccatatc tgatgggatc aatcctgtag agacaactga
    14881 tccgtcacct agtacttgtt ttactaaagc ttcacgatcg atagaataag aaatagcttt
    14941 tcttaggttc acattgttga atggtgaatt cttttcacgt tgattgaatt cgatatagct
    15001 tgtccgcgct tctttaacag aagtataagc tggatcgttt gcgttttgct gtgctaactc
    15061 accagacaag atcacgtcat ctgcttggcc atctttgaat aagttcaatg cagtagaaga
    15121 ttcttttact acactgactt tgatttcaga aagttttact gtatctttgt cccagtaatg
    15181 atcatttttc ttataagtcc attctgtatc tgttcctggt ccatcaaaat cttctaatgt
    15241 gaatggtcca ttataaacag ctttatcact tgcagatgca taggcatccc cattttcttc
    15301 cactacggat tgtttttgtg ggaaaaatga tgggaaagca agcagatatt ggaaatatgg
    15361 tgtttgtttc gtcaatttga tttctaattc atagtcacca actgctttga tccctagttc
    15421 tgatttatct ttctttccag ctgtaatgtc ttctgcattt tcaactggcg catacaggta
    15481 agcatattca gaagctgtag ctgggtctac tgttcgttgc cagccataaa cgtaatccgc
    15541 tgctgtaaca ggatcaccat ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt
    15601 taatccgtct tcactgactt ctgccaactc gctagctcct gccggctgtg gtttgctgtc
    15661 ttcatcaaga cggtaaattc cttcataaac gttattcaat gctgtaaaac tgattgtatc
    15721 tgttgcaact gataagtcag ctgtaggcat ttcttgaggc acgactaagt taaatacgcc
    15781 tcctccatct gctgaaccgc tttttgctga actactgctt gtggctgaac tgccgccata
    15841 acatccagca agtacaaggc cgcataccgc aaccaaacca aatgtaaatt tctttttcat
    15901 tttacgtacc cctttattgg aattttcaga ataattaata ttttctgaag ttgtt
//
LOCUS       DADOZM010000060        15869 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.061, whole genome shotgun sequence.
ACCESSION   DADOZM010000060 DADOZM010000000
VERSION     DADOZM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.061"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(308..730)
                     /locus_tag="J8I47_001595"
     CDS             complement(308..730)
                     /locus_tag="J8I47_001595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAZ6323033.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(744..2150)
                     /gene="atpD"
                     /locus_tag="J8I47_001596"
     CDS             complement(744..2150)
                     /gene="atpD"
                     /locus_tag="J8I47_001596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAZ6323034.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2173..3075)
                     /locus_tag="J8I47_001597"
     CDS             complement(2173..3075)
                     /locus_tag="J8I47_001597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAZ6323035.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3089..4645)
                     /locus_tag="J8I47_001598"
     CDS             complement(3089..4645)
                     /locus_tag="J8I47_001598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAZ6323036.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4668..5210)
                     /locus_tag="J8I47_001599"
     CDS             complement(4668..5210)
                     /locus_tag="J8I47_001599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAZ6323037.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5197..5721)
                     /gene="atpF"
                     /locus_tag="J8I47_001600"
     CDS             complement(5197..5721)
                     /gene="atpF"
                     /locus_tag="J8I47_001600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAZ6323038.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5812..6027)
                     /gene="atpE"
                     /locus_tag="J8I47_001601"
     CDS             complement(5812..6027)
                     /gene="atpE"
                     /locus_tag="J8I47_001601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAZ6323039.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6080..6799)
                     /gene="atpB"
                     /locus_tag="J8I47_001602"
     CDS             complement(6080..6799)
                     /gene="atpB"
                     /locus_tag="J8I47_001602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAZ6323040.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7101..9944)
                     /gene="rnr"
                     /locus_tag="J8I47_001603"
                     /pseudo
     CDS             complement(7101..9944)
                     /gene="rnr"
                     /locus_tag="J8I47_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9947..10693)
                     /locus_tag="J8I47_001604"
     CDS             complement(9947..10693)
                     /locus_tag="J8I47_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ6323041.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10805..11041)
                     /gene="secG"
                     /locus_tag="J8I47_001605"
     CDS             complement(10805..11041)
                     /gene="secG"
                     /locus_tag="J8I47_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAZ6323042.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11159..11437)
                     /locus_tag="J8I47_001606"
     CDS             complement(11159..11437)
                     /locus_tag="J8I47_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAZ6323043.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11550..12518)
                     /locus_tag="J8I47_001607"
     CDS             complement(11550..12518)
                     /locus_tag="J8I47_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6323044.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12733..12777
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12801..13775
                     /locus_tag="J8I47_001608"
     CDS             12801..13775
                     /locus_tag="J8I47_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAZ6323045.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13780..14355
                     /locus_tag="J8I47_001609"
     CDS             13780..14355
                     /locus_tag="J8I47_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ6323046.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14422..15561)
                     /locus_tag="J8I47_001610"
     CDS             complement(14422..15561)
                     /locus_tag="J8I47_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAZ6323047.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4332 a         3425 c         2842 g         5270 t
ORIGIN      
        1 gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa tcatctgacc ctcggtatta
       61 gctcaaaaaa cggcaaaata tgaggaacta ttttccattt gtttctctaa tactcaggac
      121 aaaacgctga tttcacaacc tccgatatac ggtgttccag aagtagcttc ttcgaaaaat
      181 aagctgagat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatgtcttga
      241 agctgaccac ttctgtcata acctcttttt actgttctta aacacggata aacatccgca
      301 tctcatttta tgagtgctta gatactttga tacgattgat tgctcggtgc aacgcaacag
      361 ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc ttcttcgatt tggcgttctg
      421 cgcgttgttt tgcacgttcc gcacgagata catcgatatc acgttcacgt tccgcactat
      481 cagcaacgat tgaaacaaca ttatcacgaa cttccatgat tccgccatta acagcgatcc
      541 aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac ttcatcaatc gtcaatggaa
      601 cgatgattgg tgcatgttta ggcaaaatcc caagctcacc agctgtcgta cgtgcaacga
      661 cgatagctgc atggtgatca tagaccaaac cggcaggagt taccacattt actgttaaat
      721 attgatccat ggggcacccc tttctagtag cctagtgttt tcgctttttc tacgacgtct
      781 tcgattcggc caacacttcg gaaagcttct tcaggaagat catcgtattt gccttccaag
      841 atttcgcgga atcctttcac tgtttcttct acagggacat atgaacctgg aagaccagta
      901 aattgttcag ccacgttaaa gttttgtgac aagaagaatt ggactctgcg ggcacgagaa
      961 actaacactt tttcttgatc ggataattca tccataccta agatagcaat gatatcttgt
     1021 aattcacgat atcgttgcaa tacgtgttgg acttctgttg ctactttata atgttcttct
     1081 cccacgattt caggtgctaa agcacttgaa gaagaagcca atggatctac cgctggataa
     1141 atcccttgtt ctgttaattt acgttccaag ttggttgtcg catccaaatg ggcgaatgct
     1201 gttgccggcg ccgggtcagt gtagtcatcg gctggcacat agattgcttg gatcgatgtg
     1261 atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt gacccatttc tgttgccaat
     1321 gttggttgat aaccaaccgc agaaggcatc cgtcctaaca aggctgatac ttcagatcca
     1381 gcttgagtga aacggaaaat gttgtcgata aacaacagta catcttgacc ttctacatca
     1441 cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac gcatacgtgc acctggtggt
     1501 tcgttcattt gtccgaacac catggctgtt ttttcgataa ctcctgaatc tttcatttca
     1561 taatacaagt cattaccttc acgagtacgt tcacctacac cggtaaatac agaaatacca
     1621 ccatgttctt gggcgatatt atggatcagt tcttggatca gtacggtttt accaacaccg
     1681 gcaccaccga atagtccgac tttcccacct tttaaatatg gggcaagcaa atcgataact
     1741 ttgatccctg tttccaaaat ttctgtactt gtacttaatt cgtcaaaggc tggtgctttt
     1801 ttatgaattt cgcttctttc cgcatcttct gggaaaggtg tttctaaatc gatcgtatct
     1861 cctagtacgt tgaatacgcg acctaatgtt tctttaccta caggaacaga gattgctttg
     1921 cctgtatcaa tgacttccat tcctctttgt aaaccatctg ttgattccat cgcaatggtc
     1981 cggatcactc cgtcccctaa ttctaacgct gcttcaagaa caacttttga tttattttcg
     2041 tcatttttat aaacaactaa tgcgttgttg atatctggta aggattggtc taaagaaaat
     2101 tccacgtcga caacgggacc gattacttga acaatcttgc ctgaactcat cttttttcct
     2161 ccaatctcaa tactattcta gtgccgaagc tccggcaaca atttcggtaa tttcttgggt
     2221 aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc tcatcaatga tattagcagc
     2281 attatcagtt gctgttttca ttgctgtcat accagcagca tgttctgctg ttttggcgtc
     2341 aacgatcgca ccgtagatca aactttcagc atactgaggc aataactgag acaagatttc
     2401 ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta gttgcttctt ctggatctaa
     2461 gtctgagatt ggcagcattt tttctacacg aaactgacta gttagcgaat tgatatgatg
     2521 gttataacat acgtataatt catcgaatac ttcgttttgg tacatagttg ttgccatatt
     2581 tacgattttg cggacttcat caaagcttgg ctgatcagat agattgcgca gctcatatgc
     2641 tagattgata ccgcgtgctt taaagaagtc tgcaccagtt ccgccaattg cgatcatcac
     2701 gtaatcatcg ggtgattggt ggtcttcttc taaaatagac atcgtttgtt tcaatatcga
     2761 gctgttatat ccaccgacta aaccgccatc tgcagtgatg acgatataac ccgttttctt
     2821 aacaggacgg ctgagcaaca tgctgttgta attgatatcc cctctaggat tttctgaggc
     2881 aatatcattt aactgagtag cagtcaaatg cgtaacgatt tctcttactt ttgttgcata
     2941 aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac ttagaagcag acaccatctg
     3001 cattgcccgc gtgatttggc tcgttttttt cgttgaagcg atacgtgtct tgatttcatt
     3061 taatgaagca cccattcatc tcacctcact atgcattttg aatggatttc aatttatctt
     3121 ctgtagagga attatttcct tttgtagcta agaacatgtc tttatattcc tgaatcgcac
     3181 tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg gattgtttca aagatatctg
     3241 aatgattggt atccagatat tcgaataatt catgttcgaa gtctaaaata ctgtctacag
     3301 ggatactatc taagaatcca tgtgtcaaag catacaaaat cactacttgt ttttcaacag
     3361 ctagcggtgc atgcaatttt tgtttcaaga tttctactgt acggcgtcca cgatttagtt
     3421 ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt aaatgcttct aattcacggt
     3481 aacttgctaa gtctagacgt aaagtcccag caactttttt cattgctttg atttgcgcag
     3541 aaccccctac acgagataca gatagtccgg catcaaccgc tggacgagta cctgcgtaga
     3601 acaagtcgct ttctaagaag atttgtccat cagtgatcga aataacgttt gttgggatat
     3661 aagcagagat atctccagct tgtgtttcaa caaatggtaa ggcagtcatt gaaccgccac
     3721 ctaaatcatc acttaatttt gctgcacgtt ccaataagcg tgagtgtaag tagaaaacat
     3781 cccctggata agcttcacga cctggtggac ggcgaagcag caaggaaagt tcacgataag
     3841 caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac atgtttcccg ttgtacatga
     3901 actcttcacc cattgcagta ccagcatatg gtgcgatata aagcaacggc gctggttgag
     3961 acgcaccggc attcacaacg attgtgtaat ccattgcgcc atattttttc aatgtttcaa
     4021 cttgtgtacg aactgtagaa tctttttgtc cgattgctac atagatacag atcatatctt
     4081 gacctttttg gttgatgatc gtatcgatcg caatggaagt tttccctgtt ttacggtcac
     4141 cgataactaa ttcacgttgg ccgcgtccga ttggtacaag agcgtcgatt gcttttagac
     4201 ctgtttgcat tggttcgtta acagatttac gttgcataac gcctggtgcc attgcttcta
     4261 ctggacgagc tttgtcagta acgatttcac ctagtccgtc aattggctga cctagcggat
     4321 taactacccg tccaatcaag gcctctccaa ctggaacttc catgatcttt cctgttcgtt
     4381 ttactttatc tccttcacga atggactcga aatcgccaag gataatgata cctacatcat
     4441 ttgattctaa gttttgcgcc attccgtatg agccatttga aaattcaagc aactcaccac
     4501 tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc acctacataa gttacggtac
     4561 cgatttcttc gactgaaagc acgttttgat aattttcaat ttgttcttta atcaaggcac
     4621 taatttcttc tgctttgatg gccattcgat tcacctacct ctttgtacta tctatttaat
     4681 tgattacgca tattttctaa ttgcgtgcga atgcttccgt cgattactcg atggttagct
     4741 tcaacaatcg cgccaccaat aatagaagaa tcaacgattt gttttaattc gactgattga
     4801 tagttcattg tttttgcaac attcttttct aattgtattc gttgttcgtc tgaaagtgga
     4861 actgctgttg tgacacttcc caacagcaaa ccttgatgtt catcatatcg acgttcatat
     4921 tcttcgatca tgaacaaaag atcatccatt cggttatacg cataaacaac ttctaaaaag
     4981 tttttaacga ttccatcata gccactaaca agcttgtcca tgatttctcg tttttcatga
     5041 ggttctagac gaacatcact taacatatta cctaatccag gtacttgttc atagatttga
     5101 cgtaagctta cgagatcttg ataaatttct tctgctttat ttgaatcgat cgccagttca
     5161 aataaagctt tcccgtaacg tcttcctact gtatatttat ctagtttcat ttgaagaacc
     5221 tagaccttcg atatattgat tgattaatga ttcgtgcatt tctggagata attctttgtt
     5281 caagattttt tccgcgattt gaagagatag ttctgcaaca tcgtctttta cagagttcag
     5341 cgctgaatca cgttctacag tgatatcagc ttgggcttta ctttttagac gagctgcttc
     5401 ttcttgagca tccttcaaaa tattttggcg gcttaattct ccactttctt tcgcattttt
     5461 gatgatatca gctgcatcag aacgagaggc taacaattgt tgttcgcgtt cttgttccat
     5521 tttcgctgag ttgatgcgag attgttctgc agaatccaaa tcattggcga tcttgtcttc
     5581 acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa tgcttcaaga gagccagtag
     5641 tatcaaaaat gagccgctga caacgatgac atttcctaac atcgcattgc caacttctgc
     5701 aattgccaat tgattcagca taggcgagcc ttcctttctt tgaagtactt ctagttttca
     5761 tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa tactgattca attaaacacc
     5821 aaaaactaga atcaaggcga caactacccc taagatagga acagcttcga ctagagctac
     5881 cccgataaac attgttgtac ggaattgacc agacatttca ggttggcgag ccattgattc
     5941 gattgtttta gagataactt gaccgttacc ataaccggct ccgattgctg ctcccatgat
     6001 tgcgattgct gctgcgatat aattcataat tgttttcctc ctaaattaga taaatagtag
     6061 tttgttttta aaacgaatat tattcttcca cttcaatctt atggctcata taaaccatag
     6121 ataaagtaac gaagacaaat gcttggatgc ttccgataaa caaggagaat gcgatccaga
     6181 ccatctctaa tggaattgct aacggcaatg agaaccagcc taagcttatt accatttttg
     6241 caatcaaggt tagcaaaact tcccccgcat aaatattccc ataaagacgt aaagcaagtg
     6301 ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa cataaaacta tatggtttca
     6361 agaacgaatt tacgaaatat cctttgaagc ctaacttttt caccccaaat aagtgagtaa
     6421 gtaagatcat tataaatgcc aaagttaaag taacaaacgg gtctgctgtc gggcttttcc
     6481 aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa accgatgttg tttgcaacaa
     6541 aaacaaataa aaacaatgta aatgccaaca aatggaaatt gctcaattct tttctaggca
     6601 gattatctgt tacgattcct cgactaaaat caatgaccca ttctatcgca ttttgtttgc
     6661 ctgttggttt caacttaaga tttcttgtaa aaaagtaagc taagaagaaa acaatcaaac
     6721 aggtcagtaa caccattata caaactgtac catcaaacca aattggtcca atgtggaacg
     6781 ttagcgagcg ttcatccaat ccatttcacc tcttttcccc gaaattcaca taaaatcaat
     6841 tcatgtgcgt atctggtttt ttccaatcgg tttctaacca cacggtatca taccaccatg
     6901 aaacagaaaa ttcaaaacat tttcatgaaa attagacact tttttatttc agtcaaaaac
     6961 gaagcgaatt tttctttata cacggtgctc ctgatctaag aaaaaaccta tgataatcat
     7021 gttttatcag attaaatgct aacaaatttt atcattaagt aaaagcaaaa gcaccgaata
     7081 tgcgaaaaag gataatgaaa ttatctagag aaatttttta atgttcgatc aatttgtcga
     7141 tcgatatctt ttcgtttcaa atcttcccgt ttatcataag attttttccc tttcgccaga
     7201 ccaatcaata ccttggcata gccgttacga atatagactt ttaaaggaac aacagtgatt
     7261 ccagtatttt tcaattcatt ttcaagccgg atgatttgtt ttttgtgtaa taataacttt
     7321 ctcgtgcgca acggatcatg attaaaaata tttccttgtt cataaggact gatatgaaca
     7381 ttatgcaaga aagcttctcc gttgcggacg cgaataaatc catcttttag attgatccgg
     7441 ctgtttcgta tcgacttgat ctcggttcct tgcaagacca tccctgcttc catcgtgtcg
     7501 atgatcgaat aatcatggcg agctttcttg ttttgtgcaa ttaattttcc ctcgcctttt
     7561 ggcatagctg tttcctccaa ctcattttat tttttctttt tcttcttttt aacacctttg
     7621 taaaatggct tcttcccttt tttcttaccg aacgtttgct tactatcgct atatcgtttg
     7681 ttcttcttgc catttctttc tgggcgtttt cctgcttgtt ttccttcgcg ttttcctgat
     7741 tttccatttc gtgtttttgg ccgttccaac gctgtaacct cttctgcgga aacaagttca
     7801 aaatctactg ctcgttcatc tggatcagct ttgactactt ttactcgcac tttctgtccg
     7861 attttaaaag tcagaccagt tctttcacct acaagagcta aatgattttc aacaaaatgg
     7921 aagtagtcct gtttcaattc attgatatgg atcaatccct ctactgtatt cggcagttcg
     7981 ataaacaggc cgaatttcac aacagagctg atgatcccat cgaattcttc ccccactttg
     8041 tccatcatga attctgcttt cttcatggaa tctacttcac gttctgtttc tactgcacga
     8101 cgttccatct tagaactatg atctgcaatt tcaggaagtt ccgtagccca cgaactttga
     8161 ttgctttcac ttttatcttc actgtaagaa cggatcagac gatggacgat tagatcggga
     8221 tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg ccgctaagcc ataatgccca
     8281 aaattgtctt ctgaatagcg tgcttgttgc atgcttctta gcaacatagt attgataact
     8341 gcagcttctg gtttgtcttc tactccttcg atcacatgct gtaaatcttt tggtgtgatc
     8401 gttcctttcg ttcctcggac cagaatgccc aaagctgaag caaaatcaaa gaagcgctgc
     8461 attttttctt cttttggctg ttcatggatt cggtaaataa acggcagatt taatttatta
     8521 taatgttcag ctaccgtttc attggccgca agcatgaaag attcgatcaa gcgttcacca
     8581 attcctctgc ttcttagctg gatatcattt gggtgtcctt cagcatcaac aaggattttt
     8641 gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc gcatcgtttc aaggattcga
     8701 tgcaattcac ccatttcttg gaacataggt acgagctgag catagcgttg catcgtttct
     8761 tcgtcttgat cttctaagat ttgattgaca gctgtgtagg tcatccgttc tgttgtttgg
     8821 atcacacttg ggaaaatatc atgagagact acttgtccat ttggatcaat ctccatttcg
     8881 caactcatgg tcagccttgg aacatgggga ttcagtgaac agataccatt cgataaacgt
     8941 tgcgggatca tcggaatcac tcgatcagtc aaatatacac ttgtccctct ttcatatgct
     9001 tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa catctgcaat gtgtactcca
     9061 agaaaataat taccgttatc taatttttta accgtaactg catcatctag atcttttgca
     9121 tcttctccat caatcgtaac aatgagctga tttcttaaat ctctacgtcc agttatatcc
     9181 ttttcgctga tttgatctgg aacttcatct gcttcagaca atacatcatc aggaaatttc
     9241 gtagggatac cattagccac gacaattgag aggatatcca tgccagggtc attcttatga
     9301 ccaatcactt gcttgattat cccttctaaa ctagtcgcat accctttttc agggtaatgg
     9361 gtcagttcga tcagcacaat acttccgtca actggacgaa ctccctctgc tgctgcatat
     9421 actttaaatt ggttgagctt tctatctttc gggataacaa cgccgtaaag atccgtttct
     9481 tgtatttctt cttctgaata agcaacgaat tcccctgcta actgagtagt cgcccggtct
     9541 cgaatctcga ctactttccc ctctgctccg cgatccatat ctgtatttgc tcgcttcatg
     9601 atatcgattg ctacggtgtc tccatccatt gcaaaaccag tcgcttcttt tgggatatag
     9661 atgtcatctt cttcgggatc aatcgttaca aatccaaatc ctcgttcatt tgctcgaaat
     9721 gttccttcga tcaagattgg ttttaaagga agtttcactt ttcctttttt attgaaaact
     9781 atactctgtt ctctttccat ttgagcaatc gtttgtacta gtaatttaaa atcatcgctt
     9841 ttttggaacc ctaaaccttc agcgatttct tccatagaaa aactgcgttt gcgactgctt
     9901 tccatgaaaa agagaatttt ctcttttatc gtttcttttg tcatatttat tcctcattcc
     9961 aaggtaattt ttctaaaaat tccaagacgt ctttttctaa ttcgcgtctt gctgtaccaa
    10021 ctgtaatcac atgaccactt tcaggatacc atcgaagtgt cactcgctga cggctcaact
    10081 tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc atctttccca gcttgagcta
    10141 aaaatatcgg ctgttggata tctgacaacc tactttctac gattgctgcc tgatcttgta
    10201 tatccattaa ttgttgttca gctaacgagc ggtaagcaac tgctttttca ttgatttctt
    10261 tctctgattt acctgctgtc ttcagcactt gacgtacgta tttttcaaag ttttcaggca
    10321 cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc gccgccaatc actgattctt
    10381 cttctagtgc tcgtacagca aagatgccgc ccattgataa tcctaatacg gcaatatcag
    10441 aaaactgctc agactgtaaa aaatggatgg cttttttagt gtctgcccac cagctttctg
    10501 ctttttcttg caaaatatca tatggctcca ttgttccgtg tcctttaaaa agcggcgcat
    10561 aaactgtata atctgctttt tccaaaaatc gtgcgagcat tcgaacatcg tttggacttc
    10621 ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg ttttgcataa aggggttttg
    10681 gtaattgtct cactcgtctc acctctttcg cctcatttta ccatagtttc tttcataact
    10741 tcttcttaag cgttctcttt gcaataaaaa aagccgcccg ccgttttgcg agaggcttat
    10801 tcaactactt cgaagataaa tatgctagga caaataataa gatcatccag atagctccca
    10861 taactgcagt tgatcgctgc atgacagctt caaagccgcg agctttttgt ttgccgaata
    10921 gttgatccgc accgccggta aatgcactcg ctgcactatt ttgcttgctt ggctgcatca
    10981 taatcgcaat gaccataaca atcgataaaa cgatcacgat tcccaaaatt aaattataca
    11041 tatcgcagcc tccttcccaa aagatatcgt ctctttaact ttatcataga taaaagtccg
    11101 tgacaactct atataaatgg aataacagct gaaagttcaa aactttcagc tgttattctt
    11161 atgactgacg taagacgtct tgatattctg gtttcttttc aaactctttt tttgcataag
    11221 ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa ttctactcca gtatacacta
    11281 gcttttcagc taacttttga ccacgatatg ctggatctac aaatgtatgg tcaatgatca
    11341 tgatgtctgg gcccgcatct gaccatgtca tttctccgat ctcttgttgt tcatcattga
    11401 ataaagcaag acgatttttt tcttctttga tttccatatg ccgcactcct caaataactg
    11461 atattttttt attcctacct tattttagca gtctcctgat ctataaacaa ttatttatcc
    11521 tcgaattact tacaaaaaag aagagaaaat taattcatcg ttattctttg ttcaataaat
    11581 gaaaaaatca ctgtccaata ggttttcgga tctttcatcg atgcttccaa gtgttctgct
    11641 tctggaacaa gcagcagtgc tttatcccct gccgtagcat tttgaatcgt gtacgctgct
    11701 tctgtaggga caaaatgatc tctttcacca tgaatgacca ataaaggcag atggttgctg
    11761 ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac cggcgtattt ctgggcataa
    11821 tgattcgcat atctcatgac cattttttta ggaaagacag tgattttact taacatatat
    11881 cggaattctg cataaacaga aacatatccg ctgtctaaaa taaatccttt gaccgctgat
    11941 ggaagttttt caccacttgc catcatgatc gtagaagctc ccattgatcc gccgtgtaaa
    12001 ataatggacg cattcggctg ttcatctaag atgagttgga tccaggcaat taaatctagc
    12061 cgatctagcc atcccattcc gatgatttct ccttcacttt ctccgtgcgc acgtaaatca
    12121 ggtacaagaa cgttgaatcc tttctctgca tatcttttac ctatatgaga catgtctctt
    12181 ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt tattctgctt atttggatgg
    12241 atgaacatct gaccagctag tttcaacccg tcataacttt tgattgtttt gggtgttccc
    12301 tgctcccaaa agctttcaca ctcttcttct aatttttgtc gttttttttc tactgacgtg
    12361 actgcagcag ggtccatcaa acgatgacca gtgtcttcat accagtcata tcctctctgt
    12421 actcccgctc ttactaattt ctttgcataa aaataagaaa gaaaatggta actgatccct
    12481 ataattatca gaataagaat gaaataaaaa ataaccattg tacacccgcc gctttctgcc
    12541 aaactataat ctaactatta tccaatgtac cgatccatag gattctttat tttcggcaca
    12601 cgtaaattcc atttcttttt cattgtatca tttacttaaa gttctcctca atagatttac
    12661 ttgtaagaga gcatttacat tcgcaataaa agccagtata ctaatttcgt gcattataat
    12721 caagacgctc gtcttggact ggttcggaaa cttcccagaa taaaaaacta agtatctcta
    12781 aagaaaggag attggaaata atgcgaaaag taattattga ttgcgatcca gggatcgacg
    12841 acactttagc tttatcatta gctgtcaaat cccctgatat cgaagtagtt gctattaccg
    12901 tagtttgtgg gaatgtccct gcagatatag gtacgcagaa tgtgctgaaa tgcctggaac
    12961 gctgtgatcg tttagacatt cctgtttatc aaggaatgga aaaacctttg aagcaacctt
    13021 tcatcagtgc acaagacact catggtatgg atggtttagg ggaaacaaat ttcccgatga
    13081 tcttgagaaa acaggcagaa cctctccatg ccgttgattt tttggctgat tatttcaaag
    13141 aaaagacaaa tacatcagtc attgctttgg gtcctttgac caatatcgct tttgctttga
    13201 aagtcaaccc aaatattggg aaacatatgg atcgttttgt ctcgatgggt ggaacttaca
    13261 aaagccatgg taactgttcg ccagttgctg aatacaatta ctggtgtgat cctgaagcag
    13321 cttcttatgt attcgagaat ctcaaacaaa cgatagaaat ggttgggtta gatgtgacaa
    13381 gagaaatcgt cttgacaccg actatcctcg aatactgctg tcaaatgaat cctgaagagg
    13441 gagagtatct aaaagcgatc actcgttttt attttgactt ccactggaaa caagaaagaa
    13501 ttttaggatg tgtcatcaat gatccgttag ctgttgctta ttttatcgag ggggccatct
    13561 gtgaaggttt caaaagcttc actgctgtgg aaactcaagg aataagtcgt ggacaaacat
    13621 tggttgatcg ttatgaattt tggcagaaac ctgctaacag taaaattatg acaaaagtag
    13681 atactcgtct ttttttccga aagtttttaa ccgttctttt aaatgcacaa gaagaaacga
    13741 ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa tgaataaaaa caaaatggca
    13801 actcgcgtac ttacagtgat ggctttgtgt attgggatca attatattgg aggaacgatt
    13861 gctttatggc ttcgtttgcc aatctatctc gattctatcg gaacgatttt tgctggtgca
    13921 ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca gcggtatttt aagtggagtg
    13981 actacagata ttttttctct gtattattcg ccaatccaaa ttctcacagg cttgcttgct
    14041 ggattgattt tgccaagata tctcggcaaa gataaaaaca gcttgaaact gttattactc
    14101 gctttgattt tgtcggtacc tggaacgatc gtttcttcga tcattaccgt tcagttattt
    14161 ggcgggatca cctcctcagg atccagtatg atcgttcagc tattacatgg gttaggttta
    14221 agtcagacaa caagtgtgat cttggtacag gcaggtacag actaccttga tcgtttgttg
    14281 tctatcgtcg ttgtttcatt agtcatcact atgttgccac aacgcttgat atattcaaaa
    14341 agaaaaatag gctgaataaa cagagctgga ttagcaaaat aatgctaatc tggctctgtt
    14401 ttttcattta ttaaaaaaat cttacgctaa gtaggataaa ataattcctt gatatttttc
    14461 aatcatatcc aaataattat cgatttcaac atactcatct acttgatgag gcaaagtaac
    14521 gacaccaggc ccaaagacga caaagtcgaa ggaatggtta gctttcgtaa attcggctgc
    14581 atccgttgtt gctgccgctc caactaaagg taatggctgg ctgaattgtt gctgaatgca
    14641 atgaatcaaa ggagaatctg gatcagcttt gacaggtatt ttgttgtaat cgatcatcaa
    14701 ttccaaatga tagtctgtct cttgattcaa ttcattgacg attgattgca gcaatgcaat
    14761 gattttatca tttgggtatt ctggaatcga tcgaatgttc ccttggagct gcgcatggct
    14821 tgggatgctg ttgacttgat tcccgccact gatcaatgta acattatgga ttgtttttcc
    14881 taggacagga ttctcgattg tttcagcgag gtgattcatt tcagcatttg ctttcgtaat
    14941 gaattcattt aggtgattga ttgcattata accttggtct ggcatagagc tatgcgcttc
    15001 ttttccgtgc gaggtgactg tatagttgat tgatcccatg tgcgtataca tcaaactgta
    15061 attagttggc tctccgatga tcaaggcatc taagtcatct acatagcctg ctttcgtgag
    15121 ctgttcccca cctagttcac ccacttcttc tccgacagtg gctaataatt tgactgttcc
    15181 gttaaatggt tttcctgact ctttcagttc gatcatggca atcaccattg ctgccaagcc
    15241 actcttcata tctgtagcac cacgtccgta caaacggttc ccatggattt ctgctgcaaa
    15301 gggtgcatag gtccaagacg attcatcacc agccgccacg acatccatat gcccagatag
    15361 gcctaacact tttcctgatt ggcccttttg gtaacgagca atcaaattat ctctaccatc
    15421 tctgtaagaa acaatctctc ctgttatgtc atgctttgcc aaaagcttat tcaaataggc
    15481 tgctacttca ccttcgtttc cattcactga tttaattcgg atgatctctt gtaaaatcgc
    15541 gattttttct tcttttttca tctaatcctc tcccttgcca tttcttttta ttgtatttga
    15601 gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa tgaagaaaga gagccacttt
    15661 atgaaaaata tgctgctact tccctcctat tttatttata cgagaggact tgctcataga
    15721 ttcctattct actgttctat ccttaaaagc tcttatatga tgctagttat tgttagcttt
    15781 cctttttcaa ggatgatctt ttcttgtacc attctttgta aacatcatat acagaagcta
    15841 gttgcaaaaa aaactctcta cctgctgat
//
LOCUS       DADOZM010000061        15665 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.062, whole genome shotgun sequence.
ACCESSION   DADOZM010000061 DADOZM010000000
VERSION     DADOZM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.062"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            83..1081
                     /locus_tag="J8I47_001611"
     CDS             83..1081
                     /locus_tag="J8I47_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6323048.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="J8I47_001612"
     CDS             1105..1389
                     /locus_tag="J8I47_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323049.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="J8I47_001613"
     CDS             1470..1868
                     /locus_tag="J8I47_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6323050.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="J8I47_001614"
     CDS             1878..4421
                     /locus_tag="J8I47_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAZ6323051.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="J8I47_001615"
     CDS             4443..6785
                     /locus_tag="J8I47_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323052.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="J8I47_001616"
     CDS             6801..8975
                     /locus_tag="J8I47_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAZ6323053.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="J8I47_001617"
     CDS             8965..9387
                     /locus_tag="J8I47_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ6323054.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="J8I47_001618"
     CDS             10330..11241
                     /locus_tag="J8I47_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323055.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="J8I47_001619"
     CDS             11728..11994
                     /locus_tag="J8I47_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323056.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="J8I47_001620"
     CDS             12066..12407
                     /locus_tag="J8I47_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323057.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="J8I47_001621"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="J8I47_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ6323058.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="J8I47_001622"
     CDS             12958..13254
                     /locus_tag="J8I47_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323059.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="J8I47_001623"
     CDS             13269..13700
                     /locus_tag="J8I47_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323060.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="J8I47_001624"
     CDS             13882..14187
                     /locus_tag="J8I47_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323061.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14591..15079
                     /locus_tag="J8I47_001625"
     CDS             14591..15079
                     /locus_tag="J8I47_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ6323062.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            15066..>15665
                     /locus_tag="J8I47_001626"
     CDS             15066..>15665
                     /locus_tag="J8I47_001626"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323063.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELN"
BASE COUNT         5558 a         2346 c         3259 g         4502 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata acccaaatat
    14401 gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg aaaaatgatt
    14461 ctatggacgg ttattgattt atatttacca aatggctaat aaaatagtat ttcttatatt
    14521 gtatagtttt tgtttttatg tgaaaacatg agcaatagaa ttgtactagt aggataagga
    14581 tggatggaaa atgaaaaaaa ctatttttgt tttagcgatt tttatatgtt ttgggctact
    14641 tttggtaggc tacttgttgt ttaattctaa tgaaatgaga actatttcta cgaatgactt
    14701 gttaaaaaaa agtgaattaa aggataaaaa aaaagaatct cttgccttta taatgtttac
    14761 tcaagaagag tgtgagtatt gtatagcaat aaatccaatc atagatagtg taaataagga
    14821 aactttgaat aagataaaga aatatgatat atctgtagat tttcaatcta aacaaaagtg
    14881 taaaattaag tttgttccga ctattgttgc atatagtaag aataaagaag tggggagaat
    14941 tgacaatgaa ttagataatt tgtatcgaaa aaagcaagat ggttcaattg taatagacaa
    15001 gaaaaaaaca aagacattgc ttatagattg gatgaaaaaa atggataaac aaggagaata
    15061 tttgtatgga gaagattaaa cagacatttc cgttaattaa taaatattgg aataagaaaa
    15121 aaatttttat attttttatt acgtttctgc tgtcatcttt attttctcta attttcccgt
    15181 atctatttag taatgttatt gatttatatt tagagaatga gttatcacag aatattgtgc
    15241 gaatagtaac gattcttata tttcagttat ttttagtctt aattaaagaa gtgtatggat
    15301 atataaaaac tatttatgca tttgaagttg atttatcaat gaaaattgat tttcttaagg
    15361 ttttacaaaa aaaaaatctt tttttttcgc aaacgaagca ttcaggagaa attcaattta
    15421 ggatgttttc ggatatttct actatcgtaa atactatctt tattttttct gttgaattac
    15481 ctgtgatagg cgttttagtt cttgtagcgg tatttattat aagtaaagtg aatcttattt
    15541 ttctgatttt ttcactaata atgatgggag ctatggtgtt acagtataat ttatttcaat
    15601 taccgcttaa gaaaacaatc gagaatacaa aaagtgttaa tcaaagtatt acaggagaat
    15661 tgaat
//
LOCUS       DADOZM010000062        15244 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.063, whole genome shotgun sequence.
ACCESSION   DADOZM010000062 DADOZM010000000
VERSION     DADOZM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.063"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(297..3575)
                     /locus_tag="J8I47_001627"
     CDS             complement(297..3575)
                     /locus_tag="J8I47_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAZ6323064.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3846..4391)
                     /locus_tag="J8I47_001628"
     CDS             complement(3846..4391)
                     /locus_tag="J8I47_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ6323065.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4403..5095)
                     /locus_tag="J8I47_001629"
     CDS             complement(4403..5095)
                     /locus_tag="J8I47_001629"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAZ6323066.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5114..5398)
                     /locus_tag="J8I47_001630"
     CDS             complement(5114..5398)
                     /locus_tag="J8I47_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323067.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5411..5974)
                     /locus_tag="J8I47_001631"
     CDS             complement(5411..5974)
                     /locus_tag="J8I47_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAZ6323068.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6202..6864
                     /locus_tag="J8I47_001632"
     CDS             6202..6864
                     /locus_tag="J8I47_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAZ6323069.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6988..8184
                     /locus_tag="J8I47_001633"
     CDS             6988..8184
                     /locus_tag="J8I47_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAZ6323070.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8285..9661)
                     /locus_tag="J8I47_001634"
     CDS             complement(8285..9661)
                     /locus_tag="J8I47_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323071.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9698..10564)
                     /locus_tag="J8I47_001635"
                     /pseudo
     CDS             complement(9698..10564)
                     /locus_tag="J8I47_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(10653..11678)
                     /locus_tag="J8I47_001636"
     CDS             complement(10653..11678)
                     /locus_tag="J8I47_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6323072.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11696..12370)
                     /locus_tag="J8I47_001637"
     CDS             complement(11696..12370)
                     /locus_tag="J8I47_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323073.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12554..13447)
                     /locus_tag="J8I47_001638"
     CDS             complement(12554..13447)
                     /locus_tag="J8I47_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ6323074.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13521..15218)
                     /locus_tag="J8I47_001639"
     CDS             complement(13521..15218)
                     /locus_tag="J8I47_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ6323075.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         4384 a         3324 c         2649 g         4887 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc
       61 ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
      121 ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc
      181 aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc
      241 ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat
      301 ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat
      361 gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca
      421 ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat
      481 tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct
      541 cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca
      601 tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa
      661 aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt
      721 tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac
      781 acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca
      841 tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg atttggttct
      901 tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca
      961 cctacagata atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta
     1021 atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga
     1081 ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga
     1141 gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga
     1201 tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa
     1261 gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca
     1321 ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg
     1381 gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc
     1441 gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt
     1501 acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt
     1561 tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca
     1621 aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct
     1681 ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg
     1741 aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga
     1801 atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta
     1861 tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca
     1921 gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga
     1981 atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa
     2041 taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc
     2101 agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa
     2161 actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa
     2221 ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta
     2281 aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata
     2341 gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat
     2401 ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag
     2461 gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc
     2521 gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca
     2581 gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg
     2641 atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgactc taaaccagta
     2701 ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacaaattc
     2761 ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa
     2821 gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct
     2881 tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg
     2941 ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct
     3001 agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg
     3061 tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg
     3121 atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt
     3181 ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg
     3241 tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct
     3301 ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc
     3361 atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg tacttcctcc
     3421 attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg
     3481 ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc
     3541 attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag
     3601 aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat
     3661 aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt
     3721 attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag
     3781 tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg
     3841 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
     3901 ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta
     3961 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
     4021 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
     4081 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
     4141 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
     4201 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
     4261 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
     4321 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
     4381 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
     4441 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
     4501 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
     4561 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
     4621 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
     4681 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
     4741 aatatagagg cattcctggt aattgtcctg gtttgtagcc aaatcctgtt gcatctgcat
     4801 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     4861 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     4921 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     4981 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     5041 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     5101 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     5161 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     5221 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     5281 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     5341 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     5401 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     5461 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     5521 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     5581 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     5641 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     5701 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     5761 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     5821 tgatcccgac accaccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     5881 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     5941 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     6001 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     6061 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttctagt
     6121 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     6181 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     6241 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     6301 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     6361 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     6421 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     6481 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     6541 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     6601 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     6661 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     6721 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     6781 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     6841 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     6901 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     6961 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     7021 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     7081 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     7141 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     7201 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     7261 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     7321 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     7381 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     7441 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     7501 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     7561 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     7621 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     7681 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     7741 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     7801 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     7861 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     7921 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     7981 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     8041 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     8101 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     8161 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     8221 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     8281 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     8341 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     8401 caatacaagt caagatggag acgattggca atagcggaac gaatggcact ttgaattctc
     8461 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     8521 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     8581 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     8641 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     8701 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     8761 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     8821 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     8881 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     8941 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     9001 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     9061 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
     9121 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
     9181 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
     9241 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
     9301 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
     9361 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
     9421 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
     9481 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
     9541 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
     9601 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
     9661 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
     9721 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
     9781 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
     9841 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
     9901 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
     9961 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
    10021 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
    10081 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
    10141 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
    10201 caataatacc ctatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
    10261 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
    10321 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
    10381 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
    10441 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
    10501 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
    10561 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
    10621 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
    10681 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
    10741 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
    10801 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
    10861 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
    10921 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
    10981 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
    11041 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
    11101 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
    11161 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
    11221 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
    11281 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
    11341 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
    11401 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
    11461 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
    11521 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
    11581 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
    11641 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
    11701 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
    11761 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
    11821 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
    11881 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
    11941 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
    12001 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
    12061 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
    12121 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
    12181 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
    12241 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
    12301 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
    12361 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
    12421 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
    12481 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
    12541 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
    12601 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
    12661 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
    12721 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
    12781 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
    12841 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
    12901 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
    12961 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg
    13021 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
    13081 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
    13141 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
    13201 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
    13261 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
    13321 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
    13381 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
    13441 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
    13501 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
    13561 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
    13621 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
    13681 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
    13741 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
    13801 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    13861 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    13921 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    13981 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    14041 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    14101 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    14161 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    14221 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    14281 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    14341 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    14401 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    14461 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    14521 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    14581 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc
    14641 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    14701 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    14761 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    14821 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    14881 tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga
    14941 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    15001 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    15061 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    15121 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    15181 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    15241 tatt
//
LOCUS       DADOZM010000063        15230 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.064, whole genome shotgun sequence.
ACCESSION   DADOZM010000063 DADOZM010000000
VERSION     DADOZM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.064"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            278..787
                     /locus_tag="J8I47_001640"
     CDS             278..787
                     /locus_tag="J8I47_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6323076.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="J8I47_001641"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="J8I47_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ6323077.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="J8I47_001642"
     CDS             2322..3302
                     /locus_tag="J8I47_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323078.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="J8I47_001643"
     CDS             3424..4842
                     /locus_tag="J8I47_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAZ6323079.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="J8I47_001644"
     CDS             4913..6382
                     /locus_tag="J8I47_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323080.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="J8I47_001645"
     CDS             6392..6859
                     /locus_tag="J8I47_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ6323081.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="J8I47_001646"
     CDS             6895..7767
                     /locus_tag="J8I47_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6323082.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="J8I47_001647"
     CDS             7848..10382
                     /locus_tag="J8I47_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6323083.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="J8I47_001648"
     CDS             10538..11311
                     /locus_tag="J8I47_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323084.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="J8I47_001649"
     CDS             11304..12095
                     /locus_tag="J8I47_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6323085.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="J8I47_001650"
     CDS             12105..12596
                     /locus_tag="J8I47_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323086.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="J8I47_001651"
     CDS             12614..13024
                     /locus_tag="J8I47_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323087.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="J8I47_001652"
     CDS             13028..14428
                     /locus_tag="J8I47_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAZ6323088.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="J8I47_001653"
     CDS             14451..14825
                     /locus_tag="J8I47_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAZ6323089.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DADOZM010000064        15067 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.065, whole genome shotgun sequence.
ACCESSION   DADOZM010000064 DADOZM010000000
VERSION     DADOZM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.065"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(221..1915)
                     /locus_tag="J8I47_001654"
     CDS             complement(221..1915)
                     /locus_tag="J8I47_001654"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAZ6323090.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1972..2100)
                     /locus_tag="J8I47_001655"
     CDS             complement(1972..2100)
                     /locus_tag="J8I47_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAZ6323091.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2099..2347
                     /locus_tag="J8I47_001656"
     CDS             2099..2347
                     /locus_tag="J8I47_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323092.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2539..2904
                     /locus_tag="J8I47_001657"
     CDS             2539..2904
                     /locus_tag="J8I47_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAZ6323093.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2901..3266
                     /locus_tag="J8I47_001658"
     CDS             2901..3266
                     /locus_tag="J8I47_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAZ6323094.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(3358..4785)
                     /locus_tag="J8I47_001659"
     CDS             complement(3358..4785)
                     /locus_tag="J8I47_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ6323095.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5255..7090
                     /gene="tdc"
                     /locus_tag="J8I47_001660"
     CDS             5255..7090
                     /gene="tdc"
                     /locus_tag="J8I47_001660"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAZ6323096.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            7395..8873
                     /locus_tag="J8I47_001661"
     CDS             7395..8873
                     /locus_tag="J8I47_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323097.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            8839..10170
                     /locus_tag="J8I47_001662"
     CDS             8839..10170
                     /locus_tag="J8I47_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323098.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            10284..12836
                     /locus_tag="J8I47_001663"
     CDS             10284..12836
                     /locus_tag="J8I47_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAZ6323099.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(12843..14282)
                     /locus_tag="J8I47_001664"
     CDS             complement(12843..14282)
                     /locus_tag="J8I47_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ6323100.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            14628..14990
                     /locus_tag="J8I47_001665"
     CDS             14628..14990
                     /locus_tag="J8I47_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323101.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         4739 a         2526 c         3081 g         4721 t
ORIGIN      
        1 gcggtaataa gtctaaccaa gtcaaaaaca taagaagctg ttttagcttg gctgctctta
       61 ttacttgatg cagggcagct ttttcataac ctcttggtaa acggtgttca aaagcttgca
      121 tctgcggtaa taagtccaac caaatcaaaa acataagaag ctgttttagc ctggttgttc
      181 ttattacttg atgcagggca gctttttcac aacctcttga ttattcaaac attttttttg
      241 cggcacgagc tagagtagga atatctttgt cgtaacgcgg tgttttgaca agacgggaca
      301 taggaatacc cgcaaaatgg aaagcagcga tttctccaga acgtacagca ctttgttcag
      361 taaagatcat ctgataaggc tgctcgacaa attctcctgt aaaggccaga ttggtcgaat
      421 gtttaggaat gacttttggt cgatcgcttt tggcacgatt attgaacaac gcggaagcat
      481 aaggcatata gacagggata ttatttatta cgctgtcgaa aatttctgtt tctttttcac
      541 gaatattcac tggtccagga tctacttttg aaagttgtcc aatcagttct tctagcattt
      601 cttttccatt cattttaata tactgtttat ccacaaattc gccacgtcgt cttggataaa
      661 ggaaataacc ccaaataact gtttcatttg gtttctgaga cgtaaaatgc ggttgatgat
      721 gcacgacaat agacatattg acatcttttt ggcctaacgg cgtgatcggt gtggttgaaa
      781 taaatgaatt caaggcattt cctggttctt gagtcgtgat acgggtgatt tcattcaaca
      841 acaaatggtt tttcgtcgtc agggtaaagc tgacccattc gcttgcatct cgatcagcaa
      901 agaatttgtc aggatttccc aagttgtaga aacgttcact ggcttttttc cagagactag
      961 aagctgcacc ataatccata ttttctacag ctggtgtttc gtaatctcct aaagtagctg
     1021 aatctgtaat ggacccatta gtgaaaataa ctgctgtatc ttcatcgacc acaacgtatt
     1081 cttcttcttg agtcagtgta ttggtaattt tcaaaccagt cacagtgatt tcatcttgca
     1141 tcggtgtttc tttgaattcc cagtcagtga cacggcggtt caaaatgatt ttgcatcctt
     1201 gttctttcaa gtactcgatc aaaggaagca taatactttc gtattgattg taacgtgttc
     1261 gattgacgcc cactagatgt tcgatttgag tgaattcata aatcatttga tgcatatagc
     1321 gacgcaattc ttgagcagaa ctacgtgttc tgaaagcaaa tgttgtttcc cacataaacc
     1381 agaagtttgt ttcaaaaaag tgtggatcat ctttgaaata ttcttcgata gtgatattgt
     1441 ctagtttttc ttcttctgaa tcaggcatag caatcagttt agtcagaaga atacggtctt
     1501 tattattgaa tcccagtttt ccgccatcaa tgattccttt gccaccttcc atcaaacgtg
     1561 ctttgtcaaa ggttcggtgt tttgcatcaa aatctctcgt atcttcttca gcagtcatcc
     1621 ctggctcagt aacagaaggt attcgactta acagatccat caaatctaca taggtacggt
     1681 aattaagcat gcgaccgcct cgagcaatat agcctttttg gttttctaat ggatggtttt
     1741 tattccaata ttcatccact acagtgtcgg tcggtgcacc atcatttgaa ccgtgatcat
     1801 ccaatgaata aaaggtgatc tgatcccctt tccatttgcc ttcttgaatc aaataaacag
     1861 ccgctgccat attggcaata ccggcaccaa tcattattgc tttacttttt ctcattgtga
     1921 ttcctccaat gtcttccaag tatgtccgta gtgattcaac taaataggat gctagattga
     1981 ttcgaattca tcatataaat gactgtcatc ttcgaatagt cctgtctttt ttcctagata
     2041 tgcagcgcca agggcaagca aacctaaacc gatgccgact ttgattaatt ttttactcat
     2101 gaaaaacgcc tccttgtttt ctctttctat atccaaggat agttcgtttt tattagaatg
     2161 ccaaatgata ggacgagaag aaaaaggaaa ttttgacaaa aaagaagaag tgtctgattt
     2221 tcctgtaaat aaaaataggg taggtaagaa gaaaagattt gaggaggtgt atggttctat
     2281 ttttagaaat ttgggtacaa aaaaagcacc gagacagtgc ttctttgaaa agctgatgac
     2341 ggactaagcc ttattttaac ttgctacgtt gttttggata cttccacgat ttatggatgg
     2401 agggagaaaa atgggagagt gaaaaagaaa aatttaagcg aaccgttatc attagaacaa
     2461 cttttaaata gacgaacaag gtaactttct tttacaataa aagaatagga acaaattaaa
     2521 agaaagtagg tagcgtatat gtttcgctca aatttaattg aatttctaga atggatcatt
     2581 gctattttaa atatttgttc tatcggtatc ttaattgtcg ggatcgttct tgttctattc
     2641 aaactttttg attggtctca tgtcaagaaa aactatgcag aacgtaatac tcgtaatgca
     2701 gatacaagaa aactgctagc aagttatatt ttgttaagct tggaagtact agtcgttgct
     2761 gatatcatcg agtcagttat caagccgact tggacagata ttttgaagct tgcacttatt
     2821 attatcattc gaacagtcat ttcttatttc ttgaatcagg aaatagaaga caaagaaaaa
     2881 caaagaagag gagaggtagg atgacgacag agttgatgca gtttcttctt ccatatttcg
     2941 aaaaacttgt actgggactt gatatctttg ctatctgttt attggtttgg ggtgtttgtc
     3001 tatcaatcaa ggattttttg aaatatttat ttgtccatac aaaagtgcaa agcgaccgag
     3061 taagcgagaa caatgctatt aaaaagatgt tgggaggcta tatacttcta agtttggaaa
     3121 ttcttgtttc agctgggatc atcgaatcga ttatcaaacc taccttgcag gatattttcc
     3181 aactagcggc gttggtagtt atccgaacca tcatttctta ttttttaaat aaagagatcg
     3241 gacccctaga tgtccaaaat aagtgaggtt gtgagcctga cgtgtatcaa gagattgtta
     3301 cagaaatggg catcttcaac aaataagagg ctgtgacatg atttttgtca cagcccctta
     3361 tttgttttgc acagctgaaa tgatagctcg atcgatcgta ttgatcgaaa taggaataat
     3421 attttcatat tggaccgcat ctggtgtata taatgtacgt tcacataata cccggtttga
     3481 ggactgctgc gcatacttct ttatatcaga tatacttgaa taaattgttg gtgcaacttg
     3541 aaaatcgaaa gtagttacag cttccaaggc ttcccgataa atcagataat gaacatccga
     3601 atttgtgacg atatagatag tcagatgttc ttttgtcaga cggagaattc cgctcaattc
     3661 gatcacaaaa gcccgcatgc aattaggatt caaccgtaga tcataaggta actggctgga
     3721 ccagctggaa aaaattgtct ggacttcttt atataaattt gtctgttcct catttaaaag
     3781 ataaaatttt tctggcatga ataattgata gttatcccaa gccgaacgca tcagatgaat
     3841 cagcgcacgt tcgaacggtt ggtttccgaa taattcttga ttgaagtgag attcaaaggt
     3901 agcaactagt tcttttattt cgggagtatt gtcaataaac acttggtgta gctgttgaaa
     3961 gtcttccgca atcaactgat aagaatggaa accataatta caaagattga atagtgtcaa
     4021 aataaaggcg aattcttctt ttttataata ggtttggaaa tcagtgtttt cccatgctga
     4081 ttcgacataa ttgtaaatag gacgtttttt gatttcttcg ataaattttg aatctattgt
     4141 cacagcacac acttgctggc gtaggaaact gatagcaaaa ccaatcgata agatttcttt
     4201 attttcttta tcataaaatc tgccactatt ttccgttacg gattcaatga aacgatcggc
     4261 acgttcaaaa aacgattcag gtagctccca agaattaaaa ccgcctaaac gatacgacac
     4321 attaagaagc agcagacgtc tttccatttc agtgaatcga ggggagtagc ggtcgatttc
     4381 gatcccgcag tcattaaagt aagctaatac ttgttttttt aatgaataag ctttgctgac
     4441 agagatgaat tcacattcag ttaattgaag ataatcaggt tcatcttctt ccaaataaag
     4501 cagacaagtt tttaaaaaca acgaattttg agacaattgc tgcatgatct tgcatcgagg
     4561 tatatgttct gaaaaatata aagtgtaacg gtccttttcc cgatgtgtat aagcgatctg
     4621 ttcttcaaat tcttttttta tgttattgat atcattgagt aaagtggttt tgcaaacatg
     4681 caggagattg gagagctttt ctagattgac ttctttattt acatagaatt gcccacataa
     4741 aaacagctgt cttaaaatat ttttctcaat atattcttca atcatagtag agcctccgcc
     4801 ttgtattttt ttgcacaata tgggaaatat tatgacatgt tttctttttg aaatcatcag
     4861 ttaatttctg aaaaagcata taaatgccgt taaaaaaggt tttttatgtt ataaaatttg
     4921 gaaaacagat aattctatta aaaaataaat tgtgaatttt taacaatata aaaaaataaa
     4981 aatatgttta gtgaatgaag gacagtcatt ttatttgttt gttcgctttt tttaggttat
     5041 ctattttaga agactgattt attgaaaaat tgttcgcttt tttgcgttga attatcagaa
     5101 aaatccgact ttatttattt ttgtttgtaa aaatctacgt ggatttgatg ttataaaaaa
     5161 aaatttctca tttttggcgc tatagtgaag aggtgttaca gataaacaca tgtatctgca
     5221 agacattgac caacaaaata tggagggaag aaaaatgaaa gatatggata tcaaggccgt
     5281 ctttataggc gacaaagctg aaaatggacc agtttataaa atgttgttga acaagatggt
     5341 agatgaacat cttggatgga gagaaaatta tattccttca gatatgcctg caattagcga
     5401 aggagataag ctcactccag attatctagc cacccgcgac catatgatag aggtcttgga
     5461 tgaagtcagt cagcgattac gcgcaggatc gatcccatgg cattcagcag gccgttactg
     5521 gggacaaatg aatgctgaaa cattgatgcc tgctttatta gcttacaact atgcgatgct
     5581 gtggaaccca aataatgttg cattggaatc ttctatggca acttcgcaaa tggaagcaga
     5641 agtaggacag gattttgctt cattattcaa tatgacagac ggatggggac acattgcagc
     5701 agatggttct attgctaatc ttgaaggatt atggtacgcc cgttgcatca aatcgattcc
     5761 tttagctgtc aaggaagtgc tgcctgaaaa agtgaaaaaa atgtctgagt gggaactttt
     5821 gaatttatct gttgaagaaa ttttggaaat gaccgaaagc tttacggacg aagaaatgga
     5881 tgaggtcaaa gcagcttctt cccgtagcgg aaaaaatatc caaagattag ggaaatggct
     5941 ggtccctcaa acaaaacact attcttggat gaaggcgtta gatatttgcg gagtcggctt
     6001 agatcagatg gttgctatcc cagttcaaga ggattatcga atggatatta atgctttgga
     6061 aaaaacaatt cgtgaattag ctggacaaaa aatcccaatt ctaggtgtgg ttgctgtcgt
     6121 aggaacgaca gaagaaggtc aagtagacag tgtagataaa atcgtccaat taagagaaag
     6181 gttaaaagat gaagggatct atttctattt acatgttgat gccgcatatg gcggctatgc
     6241 gcgttcactg tttctaaacg aagcaggaga atttgtgccg tatgcttcct tagctgaatt
     6301 ctttgaagaa catcatgttt tccaccactg cgtaacgatt gacaaagagg tatatgaagg
     6361 gttcagagct atttcagaag cggattctgt cacgatagat cctcataaga tgggctatgt
     6421 cccttatgct gctggaggaa tcgtaatcaa acataaaaat atgagaaata tcatttctta
     6481 tttcgcgcca tatgtgtttg aaaaatcagt aaaggctcca gatatgttag gagcttatat
     6541 tttggaagga tcaaaagctg gagcaaccgc cgccgcagtc tggacagcac atcgtgtatt
     6601 gccattgaat gtgaccggat atggtcaatt gattggggct tcaatcgagg cagctcaaag
     6661 attcagagaa tttctcgatc atctgacttt tactgttaaa ggaaaaacaa tcgaagtcta
     6721 tccattgaat catcctgatt ttaatatggt caattgggta ttcaaagaac aaggctgtac
     6781 agatttgaac gctatcaatg aattaaatga aaaaatgttc gatcggtctt cttatatgga
     6841 tggcgatgtt tatggtgaac ggtttattac ttctcatact acatttacac aagaagatta
     6901 cggtgattct ccgattcgtt ttgttgaaag aatgggtcta acgaaagaag aatggaaaaa
     6961 agaacagaaa atcactctgc tacgtgcggc tatcatgact ccttatttga atgatgatcg
     7021 aattttcaat ttttatacaa aaaaaatcgc caaagcaatg gaaaagaaac taaacgaaat
     7081 catccaatag aactgatttt tgtgtgaaag aataacaaga gacatatgga gaacagacaa
     7141 ttatttgctt catcaaaaca attgtatttc cccctgtttt tcatatattc ctcgccaaga
     7201 tattcttttt tataaaacgt tctataagtt ggatcgttcg tttcatgtat caaacccagt
     7261 tcactaagat cgttcaatgg atgtttcggt gatactgggt tttgtactga atcgatgaaa
     7321 ccttgtcatt tctgttcttg aacaaaaata aaagaaaaca aaaacatatg tcgctattga
     7381 aagaaggaat aaagatgaaa gatactggtt catcaaagca aatcacgacc atgaccttta
     7441 tcggaatgac ctgcgccctt gtagcaagtg tacgaaatat tccagatgta gcagcaacga
     7501 gctggacgat gattttttac atgctggttg cagtattatt gtacgctttt ccaatctcat
     7561 tgatctctgg tgaatttgct ggaatgtttc cccaaaaagg tggtccagaa ctttgggtat
     7621 cgaatgctct aggaaaaaaa tgggggttcg ttgtctcatg gctgttatgg gtccaaatgt
     7681 ttcctgggat ggtgatggtc gcttcagcac tcgctccgtt gtttggtaat attatcgata
     7741 atgtgccgct tggtttgaac agcaaattta cattagttgt cattttagtc gtttattgga
     7801 tcatcactct tttgaatttg aaatttgata tggcaaaaat cggcggaaaa gtcggtgttt
     7861 ggttaggatt gtacgtgcct ttgacgatga tgctcttatt agggtttgct gcgtggatca
     7921 aagttgggat cgtaccaacc ggaacattag gcagtttttc atgggacaaa ttaataccag
     7981 atactacgac tgcatcctca tttgtttatt ttgcgccgat catgtttatt tttactggta
     8041 tcgaaatgtc ctctgtgtat atcactcgat tggaaaatcc agttaaaacg tatattcgcg
     8101 gagtatttgc cgcgttaatt tttatatttt tcgtcaatat tttgaatgct ttagttgtag
     8161 caaatgtcgt accaaaagga caaatggaat tgaataatat cgcgcaatct atttcgattt
     8221 attgccaaat tcttggtttg ccacatctta ttgtgaatct gtttagttta cttgtcttca
     8281 ttggtgtagc agtccaatta tctgcttggg cctcaggacc agcaaagaca gtgacagaaa
     8341 gtgctagaaa aggtgcgtat ccaccaaaat tcaatttttg gaagacgaat caatttgacg
     8401 tatccaaatc cgttattttg actcagtctg tcattatctc aatctttgca cttttttatt
     8461 tgctgattcc tggcgtcaat caagcatttt tgatgctcgt aaattcaact actgttatct
     8521 actgtatcgt ctatgtcatc atggggatcg cagttttgag attgcgttat acacatgcaa
     8581 agttgaatcg accatttaga attggaaaaa aagagaaaaa gagcaatctt ggagtttgga
     8641 ctgtcgttat tgttttattt gcggcaatcg gattttctgt cggactgacg atgaaagctg
     8701 gaacatggat caatctaatt gcagtcacag ccatcagtgt cattttgttt gtagtaccac
     8761 tctgtattga aaaaataaag aaaccttctt gggaacagga agtcgccatg ctgcttgaaa
     8821 aagaaaaagg tgaacttgat ggaaaaaccg gcaatgaaac ggactctaac taatcgaaat
     8881 atccagttaa tcgcattagg tggcgcaatt ggaacaggat tatttttagg ttcagcaaag
     8941 gcaattgcat cagcagggcc agctgttatt ctcgtttatt tgatatgcgg tttggctatt
     9001 tatggtttga tgcgggccat gggtgattta tttttagcaa atagtacatt taactctatc
     9061 agtgatttcg ttgactatta tttaggcaaa aagtgggcat tttttgtcaa ttggacttat
     9121 tggctttgct ggcttggagc tggacttgcg gaattaacag caataggatt gtatatccat
     9181 ttctggtttg tacatgtacc agctttgata agcggaggta gcgcactaat gatcttaatg
     9241 gcagtcaacc ttgttgcagt caagtggttt ggtgaattgg aatctttttt ctcaatcatc
     9301 aagatcttgg ggattttcct ttttatctta ttgggtatag gattgagtgt ctcagatttt
     9361 catacccagt ctatcggaat caaagaaaat tttggacaga tcgatgaatt actaccgttt
     9421 ggctttgctg gaatggtagt ctcatttcct atggttttat tcagttttgc tggaattgaa
     9481 atgatcggct taaccgttgg cgagacaagt gatccgcaaa agaatttgaa aaaagcaatc
     9541 aactgtctgc cttggcgtat tctctttttc tatataggaa cgattttgtc gattttattt
     9601 gttgttccat ggcattgttt agatttaaaa gaaagtcctt tagtgactat gctgcaagtg
     9661 atccattgcc aaggcggagc agcaattgtc aatctaatga ttttggttgc ttctttgtcc
     9721 tcagcaaata gtgcagtcta cagtactagc aggattttat atcaaacagc aaaagaaaaa
     9781 agtttgcccc attggttttg tgcgctttct aagaaaggcg tacctttaca cggtatttta
     9841 attacaggaa tgttattttt tgccggtctt tgtctacatt tttttgtagc tgatagtcga
     9901 ttgttttttc agcttttggc aggcatgaca acgagttgtt ttctatttgt gtggtcttcg
     9961 atcttgtgtg cacatgttca atttattaaa gaaaagaaac ggaacactac acaaagatat
    10021 aaagatcttg ctttgttggt gttttttact tcaattgctt tcagcttgtt attcgaaagc
    10081 acgacacgct ttattccttt tttattggtt atctggtttg tattcttaaa ttttgtatat
    10141 aaaagaataa gcaatagaaa tatcagttag gcccagatac attttgcaaa acgaattatt
    10201 tcagaccccc ctgaaataat cgatacgcct gaaaaaactt ccctgcgtat aataacagta
    10261 tcaattaaac aggaggggat aggatgaact ggtacagatt gacggtagaa gatgtattga
    10321 aaaactatca aacatcaaca gaaggcttgt cgcaagaaga acgacagaaa cgcctgactg
    10381 atcaaggata taatgaaata aaagcaaggc agcagacaaa acgatggaaa aaaatagcaa
    10441 agcattttac tgatctgctg atgatcgtgt tgatcattgc ctcttttttg aaatttgctt
    10501 ctagtgaata tattgaagga agtattattt tattcgtagt gatcgtcaat ggactcgtta
    10561 gttactggca agaaagaaaa gcggaagaat cattgaatgg tttgaagcag ctgatgggac
    10621 aagaagcgat agtactatca aacgggatgc aagaaaaaat ccctgcgaaa gaactggtaa
    10681 aaggagatgt cattgtctta cgtgccggag atgtcgttcc agcagatatc cgattaatcg
    10741 aagcacatga tttattagtg gaagaatcga ttttgacggg agaatcagaa gcatctgaaa
    10801 aaagtcatgc cgttttgaca gaagaagaat cgatcggcga ccagaaaaat atggcttttt
    10861 caggaacgct tgtacaatct ggttctgcag tcggtgtcgt tgtagaaaca ggaaacacga
    10921 cagaaatcgg taaaatcaat caagcattgc aatcggtcga acaacaaacg acgcctctga
    10981 ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg cattctttgt ttgagcctat
    11041 tcttagttat ttttacgaca tttcgatatg gtatggattg gcatatcctt ctttcggcta
    11101 cgattgcttt ggtggtgtca atggtacccg aaggattgcc agctgttttg accatgattc
    11161 tatccgttgg agtacatgaa atggcaaaag aaaaggcgat catcaaagga cttccttcgg
    11221 tagaaacatt aggttctatg acagtgatct gttcagataa gacaggaaca ttgacaaaaa
    11281 atgaaatgac agttgtggat gtagctgcaa aagaagaagc ctgcgtgttg tccattatga
    11341 aaaactgtca ggaattaaaa acaaaagaag aacagaaaac agaaaattta agtggcaatc
    11401 cgacagaagt tgcgctgatt cgacacacag aaacagccaa tctacctttg aaaaaaatag
    11461 aagcaaagat tccgttcagt tctgaataca aatatatggc aacgatgcat gcggaggaaa
    11521 aaggagcggt gatttacgtc aaaggtgcac cagaagtatt atttgctaaa tcaactcttt
    11581 cttctgcaga acaagaagca tggagtcaga cagctgcaga atttgcgaga aaaggtcagc
    11641 gtgttttagg atttgcttat aaaaaagtag attcgaaaca ggaactcacg catgagactt
    11701 taacccaatt aacttttgca gggatcgccg gattgatcga tccgccaaaa gaaagtgcag
    11761 tcaaagctgt aaaggaatgc caacaagcag ggatctcagt gaaaatgatt acaggggatc
    11821 acaaagacac agcaaaagcg attgcggaac aaattggctt aaaacatact gccaaagttt
    11881 tagaagggat cgatttggat cttatgtctg atgaagagtt gattcagcaa gtgccaatag
    11941 tcgatgtctt tgctcgaacg acaccagaac ataaactgcg aattgttaaa gctttgcaga
    12001 aaaatggaga aatcgtcgga atgaccggag acggcgtaaa tgatgcacct gctttgaaaa
    12061 gatccgatgt aggaattgct atgggaatca aaggtagtga agtaagcaaa caggcagctg
    12121 atatggtttt aggagatgat aatttccata cgattgcaaa agccgtaaaa gagggccggc
    12181 ggattttgga taatttgcaa aaaaccatca atttcttctt gccaactgca ttagctcaag
    12241 gattgatctt gatttgggca cttatgctga atcgcccgct acctttgtcc ccagtacaga
    12301 ttttatgggt caatatggta acgacaatca ctttatctta tgcactaggg ttcgagtcgc
    12361 cagatccaga aatcatgaag cgcccaccaa gagatccaaa gcaaggaatc ctatctggtt
    12421 atcatttgtt tcggatcatc tatgtctccc tactgatcat ggttcctgcc tatttattgg
    12481 ctatgcagtt tgaagggcaa agcttacagc aaaccattct tctgcaaaat atcgtcttag
    12541 ctcaagctgt ttacatgatc aattgcagag agttgtccaa gccgtcattg aaccaaggtc
    12601 ttttgcaaaa caaagcgctt tttctttcat taggtatcct tgctttattg caagggatgg
    12661 ttatttttct accagtcggc cagcagctga tcggtacagt aagtttaacg atgacacaac
    12721 agttattgat tggagcaaat gtgattcttc tattcttagt tgtagaaata gaaaaaggga
    12781 tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag agaatacagt cgctagccgt
    12841 tactataaaa aagttttgaa agtgagttca gtcaaacgat tgagctcatt tttcgttgga
    12901 atagaagaaa tgtatacaat agggatatct agagaatcta gagggaaatt ggaaatcaag
    12961 atatctgttt tttcaaaaga aatagtggaa agatcggtta gttcgatagg taagaagcag
    13021 acacgttttc ctaagtaatc ttttaattcc tgctgcaaga acgccttcca tgaaggttct
    13081 ccttggaaaa taaaatagac attgaacaaa ttcggtttga tagaaaaaat cgtttgttgg
    13141 atcagtagag acaagagttg gagaaagtaa tttggttcat tgatttctat ttcttttttt
    13201 agatcgttca gtaaattctc tttctgttga tacaagtgtg catattgctg atcatatgtt
    13261 tctaaaaaac gatggtattg acggtccaat gcttcaatca atatcggcga ttcatgatat
    13321 tttaatatga ggagagccaa gttatcagtc agagatgttt tttttagttg atcaagagac
    13381 agctctgaag ctagttcctc tacaaacgtt tcgaggacct ctaaaaatgg agaatgatgc
    13441 aggcagcgag cggtttcttg tccatagtta ttgttgtaat tccaactttc cagaaagcag
    13501 aaagaagcaa aagaaagctc agtgtcttta agataaatac cttccgttaa atacaatttt
    13561 ttcaatgcag agtcatacag ggaggagagt gtgctgttgg tatggataga agaatctaat
    13621 cggcaattcg cttttatacg gatcgtattg ataaagaacc aacatgttcc gaaaatctct
    13681 tctgtttcaa ccgctagttc agtttgactg aggtttctta aaaattgaaa ataatgggat
    13741 tcatggatcg aataatcctc gaaaaaatag ttgttatgag tgaaaggaat cagcaatcga
    13801 tgataaaaag tacgaatcgc acgctctttt ccaagaatac aagctgtata tttcgtcaaa
    13861 tcgatctgca agtcgtaact tgctaaacgc tgattgattt tagtcacatg tcttttcaat
    13921 gtcccataag aaagcccatg gtcggctaga aaatcaggga cagcaacttt ttttgtaaaa
    13981 agcagatttt tcagcagttc gacactgact gattgtttga aaaaaatctc ccaaaggtca
    14041 tttgttaata aattttgctg gttttgcaaa ctgattcctg cttgatcgct gatgatgtcc
    14101 cagccttcgg gcagctgaga gcgaatagat tgaaggtcag aaaaaatcgt tcgttcggtt
    14161 gtccctatga tttcagctaa ttcatttacg gttatctttg tatgattcag caattgttct
    14221 actaaagaaa catgacgtcg aatatctttt tcggtaatga tttttttcat tagtgaatac
    14281 atcaatcaga ctcccttcta ttctaagaat acggagaaaa taaacggtgg tcaagtttca
    14341 actgattttt caatcattgt ttatttttct ggagcattta ctgatgataa gaatttcatc
    14401 atgaacttga aaaatgatac cactaaaatc aaaaaatagg tgggcaaaca ataataaaca
    14461 ttttaatagt agatggtagc aagaccaaca caggcggatt gaacatttaa gagttgataa
    14521 aaaagaaaaa tatcgaagta tttgattagc aactacttgt attcattttt ctattcttac
    14581 gtggtatctt tgaataaagc gaatcaataa gaaagagatg gaagcagatg ctaacgaaaa
    14641 aagaactaat cgatcacatc aatgaaacat acaatttgtc tccagaatat atttttaaaa
    14701 aatttcctga ttattgcgta ttcagacatc aaaaaagcaa aaaatggttt ggggtcctga
    14761 tgtctgttcc agaagaaaaa atctatgatg atgcaagtga ccataggatc atcaatattc
    14821 ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg aggaaaagag gggtattttc
    14881 cagcctatca catgaataaa gaacattgga tctctattga tctacgtaaa atagaaaagc
    14941 tagatgaact agcagatttg attgatggaa gttttaaatc aacgcaatga atgtctagga
    15001 caatagagaa gaggttgtga cagaaatgtt tagcttcaag aaataagaag gaatttacga
    15061 aaattgc
//
LOCUS       DADOZM010000065        14973 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.066, whole genome shotgun sequence.
ACCESSION   DADOZM010000065 DADOZM010000000
VERSION     DADOZM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.066"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..577)
                     /locus_tag="J8I47_001666"
     CDS             complement(<1..577)
                     /locus_tag="J8I47_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6323102.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="J8I47_001667"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="J8I47_001667"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAZ6323103.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="J8I47_001668"
     CDS             1633..3096
                     /locus_tag="J8I47_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323104.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="J8I47_001669"
     CDS             3093..3551
                     /locus_tag="J8I47_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ6323105.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="J8I47_001670"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="J8I47_001670"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ6323106.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="J8I47_001671"
     CDS             4963..5691
                     /locus_tag="J8I47_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323107.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="J8I47_001672"
     CDS             5709..6905
                     /locus_tag="J8I47_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6323108.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="J8I47_001673"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="J8I47_001673"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ6323109.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="J8I47_001674"
     CDS             7890..8831
                     /locus_tag="J8I47_001674"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAZ6323110.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="J8I47_001675"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="J8I47_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="J8I47_001676"
     CDS             9499..10389
                     /locus_tag="J8I47_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323111.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="J8I47_001677"
     CDS             10376..11185
                     /locus_tag="J8I47_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6323112.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="J8I47_001678"
     CDS             11199..11600
                     /locus_tag="J8I47_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ6323113.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="J8I47_001679"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="J8I47_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAZ6323114.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(13156..13431)
                     /locus_tag="J8I47_001680"
     CDS             complement(13156..13431)
                     /locus_tag="J8I47_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAZ6323115.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            13539..14303
                     /locus_tag="J8I47_001681"
     CDS             13539..14303
                     /locus_tag="J8I47_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAZ6323116.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            14426..14929
                     /locus_tag="J8I47_001682"
     CDS             14426..14929
                     /locus_tag="J8I47_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6323117.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT         4801 a         2463 c         3261 g         4448 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg atgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga
    12721 aaaaaagaca aaaaatcaag aaagaaatac ttggaaagaa aagagtgatt ccaagtgttt
    12781 ctttcttatt taaaaaacta aaaacttttt tgttctgtct ggaaaatatg ggttaagcaa
    12841 atttattgca agttatagat cagctttttt actacctatc tttaatactt gaggcaaagc
    12901 gtctcaaaaa caaccttatc gacggtgttc aaaagtcagt ttctcggtat taagtcaaat
    12961 tttatgcaaa aatgtggaaa gccattttca caaaattctc cttactactc aaaactaagc
    13021 gactttctca caaccttatt gacggtgttc aaaagcttgc atctacggca ttaagtccag
    13081 cctgttgaaa aatatggaga gctatttttc acaacctgtc cttaatgctt gatgcagacc
    13141 agctttttca caaccttatc ccaccacatc aaatttcttt cgttcctcaa tcatcgggtc
    13201 ttcttggaca tccacatact gcactcgtcc acttgggctc ggagatttct tgacttcttc
    13261 aataaatttc tggatgatgt cgtcattgcc gattgcttcg atcgtgactg agccgtcatc
    13321 ttcgtttttt accgttccag tcacgcctag ttgatcagcg accatcttgg tcatgtagcg
    13381 aaatcctacg ccttggacgc gtccttggac gttcatttta actttgcgca tgttcgtcac
    13441 ctttctttcc tattttataa attaatgata ccgatttttt taggatgaaa caagctcaag
    13501 cacctattcc atgatataat catggggagg tgaggaatgt gaaagagatc caatcagcaa
    13561 aaaatccaag aatcaaagaa ttaaaaaaac tacataaaaa gaaatatcgg gaagaaaagg
    13621 aagaatatct tttggaaggg ttccatctga ttgaagaagc tgcgtcgtct gatgcagaaa
    13681 taaaagaact ttttgtgaca cagcgaggca tgaaagaatg gggagactgg ctggccgcaa
    13741 acaaagagat ttctactttt ctagtttcgg atgaggtcat ggatgctgta tcggatctac
    13801 ctacaccaca aggaatctta gcagtgatgg cgttaacggc tgaaggcacg ccagattttt
    13861 caggaggatg gctgttatta gataatgtac aagatcctgg aaatgtaggg acgatgatcc
    13921 gtacggcaga tgcatttgat atggctggtg ttttattagg caaaggaacg gcagatattt
    13981 atagtacaaa ggtgctgcgc agcatgcagg gaagcaatta tcatttgcct gtgttgcaaa
    14041 aagatttgac agaagtgatt cctttaatga aagacaaagg gtttacagtg tatggcacag
    14101 aattaaatga agaagcacac gccttagaca aagtagaaaa atcagataac ttcgcaataa
    14161 tcatggggaa cgaaggtcag ggggtaagtc aggaaatctt gtctttgact gatcagaacg
    14221 tatatatccc gatgaaagga aatgcggaat cgttgaatgt tggggttgcg gcagggattg
    14281 ttctttatca tttttcttta tgattaagga actattaagc tttggctatt ttatccaagg
    14341 cgactagaat aatccgaaca ctggtggtat acttagatca atttcagaag aaaatgaaat
    14401 tcaaaaaggt ggcagatcga taattatgac agaaaaatgg cgtgaagacg aagaatatat
    14461 gtcttatgta gaagatcttt tagaaacaga agcagtgaaa aaattagcga attacacaca
    14521 gcatgttcat tccactcgtt tggagcattc catcagtgtt tcttactata gttatctgct
    14581 cgcaaaaaaa tggggtggca atgctaaagc aacagcgcgt gctggacttt tgcatgatct
    14641 gttctactat gattggcgta cgacgaaatt tgatgaaggc acacatgcgt atatccatcc
    14701 aagaatcgct gtaaagaatg cagaaaagat caccgatttg tcggatcttg agcgagatat
    14761 cattttgaag cacatgtggg gcgcaacgat tgcaccgcct aagtacaaag aaggatatat
    14821 cgtaacattc gtagacaaat actgcgcagt caaagaagcc gctcagccta tgtcagcaag
    14881 tatgcgtaag agatggcaac gttacttcgg aaaagaatcc tcgatttaac aggtaaaacc
    14941 aataatagac catattgagg ttgtgacaga agt
//
LOCUS       DADOZM010000066        14815 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.067, whole genome shotgun sequence.
ACCESSION   DADOZM010000066 DADOZM010000000
VERSION     DADOZM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.067"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(296..1024)
                     /locus_tag="J8I47_001683"
     CDS             complement(296..1024)
                     /locus_tag="J8I47_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323118.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="J8I47_001684"
     CDS             complement(1002..2576)
                     /locus_tag="J8I47_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6323119.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="J8I47_001685"
     CDS             2805..3743
                     /locus_tag="J8I47_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6323120.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="J8I47_001686"
     CDS             3755..4666
                     /locus_tag="J8I47_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6323121.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="J8I47_001687"
     CDS             4690..6162
                     /locus_tag="J8I47_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6323122.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="J8I47_001688"
     CDS             6213..6824
                     /locus_tag="J8I47_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ6323123.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="J8I47_001689"
     CDS             6854..8767
                     /locus_tag="J8I47_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAZ6323124.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="J8I47_001690"
     CDS             8895..11876
                     /locus_tag="J8I47_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAZ6323125.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="J8I47_001691"
     CDS             11873..13534
                     /locus_tag="J8I47_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAZ6323126.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="J8I47_001692"
     CDS             13574..14506
                     /locus_tag="J8I47_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAZ6323127.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DADOZM010000067        14678 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.068, whole genome shotgun sequence.
ACCESSION   DADOZM010000067 DADOZM010000000
VERSION     DADOZM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.068"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(131..697)
                     /locus_tag="J8I47_001693"
     CDS             complement(131..697)
                     /locus_tag="J8I47_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ6323128.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="J8I47_001694"
     CDS             complement(697..1653)
                     /locus_tag="J8I47_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAZ6323129.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="J8I47_001695"
     CDS             complement(1653..2309)
                     /locus_tag="J8I47_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAZ6323130.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="J8I47_001696"
     CDS             2679..4697
                     /locus_tag="J8I47_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6323131.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5124..7538
                     /locus_tag="J8I47_001697"
     CDS             5124..7538
                     /locus_tag="J8I47_001697"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAZ6323132.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="J8I47_001698"
     CDS             7886..9439
                     /locus_tag="J8I47_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAZ6323133.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="J8I47_001699"
     CDS             complement(9820..11604)
                     /locus_tag="J8I47_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323134.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="J8I47_001700"
     CDS             complement(11619..13340)
                     /locus_tag="J8I47_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323135.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="J8I47_001701"
     CDS             13440..14201
                     /locus_tag="J8I47_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ6323136.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4768 a         2710 c         2921 g         4279 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtaac cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14341 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14401 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14461 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14521 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14581 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14641 tgtgactttc ctcagctatc ggtgatacta ccgatagc
//
LOCUS       DADOZM010000068        14567 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.069, whole genome shotgun sequence.
ACCESSION   DADOZM010000068 DADOZM010000000
VERSION     DADOZM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.069"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="J8I47_001702"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="J8I47_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAZ6323137.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="J8I47_001703"
     CDS             complement(1993..3216)
                     /locus_tag="J8I47_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAZ6323138.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="J8I47_001704"
     CDS             complement(3247..4086)
                     /locus_tag="J8I47_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAZ6323139.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="J8I47_001705"
     CDS             complement(4103..4783)
                     /locus_tag="J8I47_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323140.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="J8I47_001706"
     CDS             complement(4785..5858)
                     /locus_tag="J8I47_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6323141.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="J8I47_001707"
     CDS             complement(6505..7176)
                     /locus_tag="J8I47_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6323142.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="J8I47_001708"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="J8I47_001708"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAZ6323143.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="J8I47_001709"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="J8I47_001709"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAZ6323144.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="J8I47_001710"
     CDS             complement(11059..11925)
                     /locus_tag="J8I47_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6323145.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="J8I47_001711"
     CDS             complement(12085..14178)
                     /locus_tag="J8I47_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ6323146.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4229 a         3109 c         2479 g         4750 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatattgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattg
//
LOCUS       DADOZM010000069        14107 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.070, whole genome shotgun sequence.
ACCESSION   DADOZM010000069 DADOZM010000000
VERSION     DADOZM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.070"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            66..767
                     /locus_tag="J8I47_001712"
     CDS             66..767
                     /locus_tag="J8I47_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAZ6323147.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="J8I47_001713"
     CDS             764..1885
                     /locus_tag="J8I47_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAZ6323148.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="J8I47_001714"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="J8I47_001714"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAZ6323149.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="J8I47_001715"
     CDS             3264..4172
                     /locus_tag="J8I47_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAZ6323150.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="J8I47_001716"
     CDS             4207..4479
                     /locus_tag="J8I47_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAZ6323151.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="J8I47_001717"
     CDS             4490..5536
                     /locus_tag="J8I47_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAZ6323152.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="J8I47_001718"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="J8I47_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAZ6323153.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="J8I47_001719"
     CDS             complement(8547..9218)
                     /locus_tag="J8I47_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323154.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="J8I47_001720"
     CDS             complement(9211..9705)
                     /locus_tag="J8I47_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAZ6323155.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="J8I47_001721"
     CDS             complement(9725..10945)
                     /locus_tag="J8I47_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323156.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="J8I47_001722"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="J8I47_001722"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAZ6323157.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="J8I47_001723"
     CDS             complement(13021..14103)
                     /locus_tag="J8I47_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323158.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DADOZM010000070        13965 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.071, whole genome shotgun sequence.
ACCESSION   DADOZM010000070 DADOZM010000000
VERSION     DADOZM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.071"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(140..1342)
                     /locus_tag="J8I47_001724"
     CDS             complement(140..1342)
                     /locus_tag="J8I47_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323159.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1345..1539)
                     /locus_tag="J8I47_001725"
     CDS             complement(1345..1539)
                     /locus_tag="J8I47_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ6323160.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1586..2434)
                     /locus_tag="J8I47_001726"
     CDS             complement(1586..2434)
                     /locus_tag="J8I47_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAZ6323161.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2639..2989)
                     /locus_tag="J8I47_001727"
     CDS             complement(2639..2989)
                     /locus_tag="J8I47_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323162.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3584..4030
                     /locus_tag="J8I47_001728"
     CDS             3584..4030
                     /locus_tag="J8I47_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAZ6323163.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4654..5646
                     /locus_tag="J8I47_001729"
     CDS             4654..5646
                     /locus_tag="J8I47_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6323164.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5662..6546
                     /locus_tag="J8I47_001730"
     CDS             5662..6546
                     /locus_tag="J8I47_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6323165.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6595..8202
                     /locus_tag="J8I47_001731"
     CDS             6595..8202
                     /locus_tag="J8I47_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ6323166.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8203..9687
                     /locus_tag="J8I47_001732"
     CDS             8203..9687
                     /locus_tag="J8I47_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAZ6323167.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9864..10856
                     /locus_tag="J8I47_001733"
     CDS             9864..10856
                     /locus_tag="J8I47_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323168.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10908..11777
                     /locus_tag="J8I47_001734"
     CDS             10908..11777
                     /locus_tag="J8I47_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6323169.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12197..13762)
                     /gene="guaA"
                     /locus_tag="J8I47_001735"
     CDS             complement(12197..13762)
                     /gene="guaA"
                     /locus_tag="J8I47_001735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAZ6323170.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13817..>13965)
                     /locus_tag="J8I47_001736"
     CDS             complement(13817..>13965)
                     /locus_tag="J8I47_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6323171.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT         4277 a         2370 c         2882 g         4436 t
ORIGIN      
        1 aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta ttcccactca
       61 atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta ttcctcattt
      121 tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag tttgcaactt
      181 tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca attccttcat
      241 cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta gaatggccta
      301 accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag ctaacatgag
      361 aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca taacgctgaa
      421 taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga ataaattgtt
      481 ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt gtatcatcgt
      541 ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga ataaaatctt
      601 tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg aagtccacgt
      661 cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa tatagccaga
      721 tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc aaaacttctt
      781 caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt gcttttgttc
      841 ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat gatttacgaa
      901 acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc aataaccaaa
      961 ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca ccgaaaaatt
     1021 cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt acgctagctt
     1081 tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca taattcgcaa
     1141 atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt gcttcacgag
     1201 ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc ctctttccag
     1261 tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta tcttgataca
     1321 cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac ctataatttc
     1381 ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg gatatccctg
     1441 atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg ttttaaatcc
     1501 tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc actttcaaag
     1561 aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga acggtacttt
     1621 cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa gcattgtaaa
     1681 aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc gtggaatcat
     1741 agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg gacttgaaaa
     1801 aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg ctgatatagc
     1861 gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa ccctgacccc
     1921 aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata atatggtagt
     1981 ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt gcttttttag
     2041 ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta tttgcatact
     2101 caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga ttgtcaaaat
     2161 tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc tgtttgcgtt
     2221 ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt ttttcaagag
     2281 taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt acttcgtttt
     2341 cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca atatacgtgg
     2401 gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg ttgcgtattc
     2461 gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag ttaaggggga
     2521 aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac ggacagaaac
     2581 ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca tagtatagtt
     2641 agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca gcggttgccg
     2701 ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc accaaattgg
     2761 taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc acaggatgtt
     2821 cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata cattcgtagc
     2881 tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact tggtattctt
     2941 tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc acaattctcc
     3001 ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat ctacgacttg
     3061 tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat atacgatttt
     3121 ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt ttggtttagt
     3181 acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg gaatgtctct
     3241 ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca gttcctacgt
     3301 tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta tcagcggaaa
     3361 acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt atttgtttct
     3421 cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa taaagcaaga
     3481 attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct atcctttgga
     3541 ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg actatgtcgt
     3601 acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat catcaaaagc
     3661 aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg agatgagtgg
     3721 tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat caataaaaac
     3781 ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa agttgatcaa
     3841 tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa tgatatttac
     3901 tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat ttcaatcgat
     3961 gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt atgattggtt
     4021 tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat ttttttcagc
     4081 ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa gtactcaaga
     4141 gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa tgtactcttt
     4201 tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga aaacatattg
     4261 attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac tggtgagtgg
     4321 tcttgaacat acttttaaat cacattttac tgagttttat tggattttat aattcattgg
     4381 aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat tccagctatc
     4441 aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat tattatcaga
     4501 atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa aaaatggata
     4561 tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg ttaaattttt
     4621 atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat tgaattggaa
     4681 aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa aaatatttcg
     4741 aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt gttgctctta
     4801 ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc tgtgattgca
     4861 tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg actagatcac
     4921 tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac attaaaaatt
     4981 agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact gagcttgaat
     5041 caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc gccgaatttt
     5101 atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa tggacctatt
     5161 aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa tccagcttat
     5221 ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc atccattctt
     5281 tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa tatcgatggg
     5341 gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc tgtgatggtg
     5401 atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa agcctacttg
     5461 atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt atacaaagtc
     5521 ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa tatgattatt
     5581 aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga tggtcaaggc
     5641 ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg tcaaatctta
     5701 tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc gctgatttat
     5761 gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat aagcttcaat
     5821 ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc cattttacgc
     5881 ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt atttattacg
     5941 atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat tattatgact
     6001 ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt attggtaaag
     6061 aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat aaatgtgtgg
     6121 aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc tgaagctgct
     6181 actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc attagcgaaa
     6241 ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc atactttgat
     6301 gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt aagaaatatt
     6361 ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat ggcagaaatg
     6421 aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct gccattaatc
     6481 ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg ttctgttaaa
     6541 gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt taagatgaaa
     6601 gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc ttgtggaggc
     6661 tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc ttttccattg
     6721 aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc caatgatcct
     6781 aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat tgattggaca
     6841 aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg tgatttacca
     6901 gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc ggaaagtggc
     6961 gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa gaaaattttt
     7021 gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat ttactctatg
     7081 ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa tgggatggct
     7141 tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac cactgatcag
     7201 ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg tgaagcagat
     7261 gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt tttaatgggg
     7321 gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg tcaggtacaa
     7381 tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga aatgaataat
     7441 aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt tgccaaaggg
     7501 aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac gggtgcagat
     7561 gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca tgttactcgt
     7621 acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa taaaaacttg
     7681 gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt ccaaaataac
     7741 tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga tggtatgtta
     7801 aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac cgaagctgga
     7861 ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc agatgatgcg
     7921 aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga agacaatatc
     7981 tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat cgaaacggat
     8041 atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa aatcgatgaa
     8101 gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg gattcaaata
     8161 aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt agaagaaacg
     8221 gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt taaccctcag
     8281 tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc caatgggttt
     8341 atatattaca aagggcatta ccatctcttc tatcaatact atccttacga tagtcgatgg
     8401 gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga agatctacca
     8461 gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg aagtgcaatt
     8521 gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga tggtattagc
     8581 catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa atacagtggt
     8641 aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga atttcgtgat
     8701 cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa atctacacag
     8761 tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca cttttactct
     8821 gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga tttattccat
     8881 ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa cggactggaa
     8941 tatcataata tcagttctac catggcttgt attggtgagg taaactggga aacaggtcag
     9001 atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc accacaaaca
     9061 acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg ggatcgtaca
     9121 ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc ccgagagtta
     9181 agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag tcatttagtt
     9241 tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt tcgaaacgcg
     9301 ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga agtttttaca
     9361 attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac tgctacacag
     9421 gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga agaagaccct
     9481 ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct tgagattttt
     9541 agagatacct catcaattga agtctttatt aatgggttag aagttatgac ggctactttc
     9601 tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag tcgtattcaa
     9661 gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga atcaattatt
     9721 taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt tcccatggat
     9781 ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat actaaacttg
     9841 tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca aaattagcag
     9901 gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa gcttctacta
     9961 gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt tatgcccggg
    10021 ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta tggcatccat
    10081 tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac tacaagcttt
    10141 atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag atggtcagcc
    10201 aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa tacatttcga
    10261 gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt tatgtgaccg
    10321 ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga ggatgtcaga
    10381 atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt cgtcgaaaat
    10441 attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat atgttagaac
    10501 ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc gatatcgatg
    10561 ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt gaaaaaatag
    10621 ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag atgacagaag
    10681 gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg gaagtggcag
    10741 tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc gtattgccga
    10801 ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt gcttagtgca
    10861 aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg ttatatggtg
    10921 gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa ttacaagtga
    10981 tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa gtgtttgatt
    11041 tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt gggcctattg
    11101 atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag ttagcttggc
    11161 aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc tattggacta
    11221 ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa ggtaaaaata
    11281 gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg gatgggaaaa
    11341 tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt cataatgaag
    11401 atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta gcatcaggac
    11461 ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat catccttatt
    11521 ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta ctatatgcac
    11581 cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc gagttgattc
    11641 gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc gaagaatatc
    11701 tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct ttagcaataa
    11761 agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc ggaagtttaa
    11821 aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga atctgagtag
    11881 agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta caagaaagtt
    11941 ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt actaaaaatc
    12001 tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa gcaaagccct
    12061 tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac catcttattt
    12121 tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta ttcctcatcc
    12181 atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg gtaatatcat
    12241 acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt ttttgcaaga
    12301 cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg acagcacgga
    12361 tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag cgaatgcctg
    12421 gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg atttcttcgc
    12481 gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat tcaccaagga
    12541 cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg tcaggcatgc
    12601 ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat ggctcgatca
    12661 gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg atggtttggg
    12721 cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca aggaaagaaa
    12781 cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca aattcatttc
    12841 caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat aagaaacgtt
    12901 ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt tccataactt
    12961 gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt aattgatcgc
    13021 cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct gaaaggccta
    13081 ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg tcgatgaagt
    13141 tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg cgcaacaagt
    13201 catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac attttgcgtt
    13261 ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca tcgaaatcag
    13321 tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt tcaggagtgt
    13381 tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg tattcacggt
    13441 ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata ccgtaacaga
    13501 ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag gccttctcat
    13561 cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt gcctttactt
    13621 cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca cgaatacggc
    13681 gagtgatcag ttggttatac tgactaccgt aatcaagtac gataattttt ggctcttcgt
    13741 caaatcgtgt tggtgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg
    13801 tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc
    13861 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
    13921 tcagcccctc agtgacgccg tttgagtggg acaccttaaa ggcat
//
LOCUS       DADOZM010000071        13895 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.072, whole genome shotgun sequence.
ACCESSION   DADOZM010000071 DADOZM010000000
VERSION     DADOZM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.072"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(27..683)
                     /locus_tag="J8I47_001737"
     CDS             complement(27..683)
                     /locus_tag="J8I47_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ6323172.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            876..1154
                     /locus_tag="J8I47_001738"
     CDS             876..1154
                     /locus_tag="J8I47_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323173.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1173..2381
                     /locus_tag="J8I47_001739"
     CDS             1173..2381
                     /locus_tag="J8I47_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6323174.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2529..2894)
                     /locus_tag="J8I47_001740"
     CDS             complement(2529..2894)
                     /locus_tag="J8I47_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAZ6323175.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3041..3814)
                     /locus_tag="J8I47_001741"
     CDS             complement(3041..3814)
                     /locus_tag="J8I47_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6323176.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3988..4848)
                     /locus_tag="J8I47_001742"
     CDS             complement(3988..4848)
                     /locus_tag="J8I47_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAZ6323177.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4848..6266)
                     /locus_tag="J8I47_001743"
     CDS             complement(4848..6266)
                     /locus_tag="J8I47_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323178.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6323..6772)
                     /locus_tag="J8I47_001744"
     CDS             complement(6323..6772)
                     /locus_tag="J8I47_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323179.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7217..7480)
                     /locus_tag="J8I47_001745"
     CDS             complement(7217..7480)
                     /locus_tag="J8I47_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAZ6323180.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7579..8874)
                     /locus_tag="J8I47_001746"
     CDS             complement(7579..8874)
                     /locus_tag="J8I47_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAZ6323181.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8871..10142)
                     /locus_tag="J8I47_001747"
     CDS             complement(8871..10142)
                     /locus_tag="J8I47_001747"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAZ6323182.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10307..11176)
                     /locus_tag="J8I47_001748"
     CDS             complement(10307..11176)
                     /locus_tag="J8I47_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAZ6323183.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11468..13078)
                     /locus_tag="J8I47_001749"
     CDS             complement(11468..13078)
                     /locus_tag="J8I47_001749"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAZ6323184.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3952 a         2928 c         2371 g         4644 t
ORIGIN      
        1 cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa caagtccata
       61 ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt cagttgtttt
      121 aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat ttcttcattc
      181 gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa atcatataat
      241 tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg ctgcattttt
      301 ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg tagctttggg
      361 tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc tgcatattct
      421 gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga
      481 gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc atttgaaata
      541 acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc catcccttcg
      601 aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt atccaaatat
      661 tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc taaaaagatc
      721 atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata ttaacatttg
      781 ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc gttatactgc
      841 agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg aacacaatca
      901 ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct ttagtctttt
      961 taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag aatccaacaa
     1021 acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt attcgccaac
     1081 ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa aaattaaaag
     1141 catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt aataaatatt
     1201 ttattggaat cacattgatc aattttattg tctacctgat ttattatctg ttgatggtca
     1261 tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca ggttttgctt
     1321 ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa cagttagaac
     1381 tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt tcaacattag
     1441 catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg aatggattcg
     1501 gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc cctgcatcta
     1561 aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc gctgttgggc
     1621 catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc tggttttcaa
     1681 ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa aatattccat
     1741 tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt attgaaaaga
     1801 aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca agtgttttat
     1861 catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca tttttcttcg
     1921 tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt ttcgatgtca
     1981 aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga ttatttttac
     2041 tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc ggcctaggtt
     2101 atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa
     2161 tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc gttggtcctt
     2221 atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg attgccggag
     2281 tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat agtcatgaag
     2341 cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga atagataaaa
     2401 aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata gtttatgatt
     2461 attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag aatagtttaa
     2521 ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag caaccacttg
     2581 tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt catccaaata
     2641 tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc tgaaccagca
     2701 tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc ccctagcaca
     2761 tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct aacaccgtat
     2821 aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac taccaacgcc
     2881 ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga ttatctaaca
     2941 agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat aaggtcctat
     3001 ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac agacattcga
     3061 tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt gtcaccagat
     3121 aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt ttttgctgtg
     3181 taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc attgcctctc
     3241 cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc attatcgcta
     3301 aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt gttgctcgtt
     3361 tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca ctcaacatag
     3421 cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct aggtttctga
     3481 ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct cgaatcaaag
     3541 aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg gtgatcttca
     3601 tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg cgaactctac
     3661 ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct tcgctataat
     3721 ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga taattcacaa
     3781 tctcctgctg tctattctcc acttcattca acataaaatc accccattta ctttatatag
     3841 taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag aagaaatttt
     3901 tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag actattgtca
     3961 taccctcttg accacacatt tttattttta gtataaacta gccttgccta ctgtgttcaa
     4021 aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg gataaattgc
     4081 attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat tgaaaaagac
     4141 gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg cttcgctgac
     4201 ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga tttgttggtt
     4261 caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat acaagccatg
     4321 cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa aaatcttcgc
     4381 ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac cattattccc
     4441 aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg caaccacttt
     4501 tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca tcacggaaga
     4561 atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat ccaagtggat
     4621 aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa actcatcact
     4681 taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta atctctctgc
     4741 ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg gtactgcaaa
     4801 ttgattctct ttagccactt tcaataactc tttcattgta tataacatta cgcttccacc
     4861 tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt cactgttttt
     4921 ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc caagacatac
     4981 ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc atgaggtgcc
     5041 attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa
     5101 ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc tgtaatatac
     5161 gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt aaatttatgt
     5221 ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc agccatacaa
     5281 gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt cgaagtcgat
     5341 ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa tccgaacaat
     5401 agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa cacccatgtg
     5461 atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga taaaaagggg
     5521 acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt atttgccaga
     5581 aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa acctgaacca
     5641 cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt tccagcaatc
     5701 gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt atctcctccg
     5761 atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc agccgctgta
     5821 atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa tggaagcatg
     5881 taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt tgcagctggt
     5941 tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc tattactact
     6001 tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc ggcaaatcgg
     6061 tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt gatttcttgt
     6121 tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg atggtttaga
     6181 tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat tccagtcggg
     6241 caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca ctatcgatac
     6301 tcggacaaag actctactat tttcattctt gactaatctg tctgatgata tctgctggcg
     6361 tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg gagagtgtgc
     6421 tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca aaaatatatt
     6481 caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga aaaaaacaca
     6541 gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca tcttctattc
     6601 ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga acatttctta
     6661 ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct tttgaatcta
     6721 tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc ataaaaaacc
     6781 tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag catcgctagc
     6841 tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc gtgtataagt
     6901 cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct
     6961 tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact tcgaaataac
     7021 ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa actatatact
     7081 agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa acgaagtttt
     7141 aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc taaacgttga
     7201 gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga
     7261 aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag aatggatgtg
     7321 agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc cattcaactg
     7381 tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt gaatccatga
     7441 atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg ctcctttgcc
     7501 ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca tcttaacatg
     7561 tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt taccaaagga
     7621 gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca aaaagcggat
     7681 cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt tccaaatttc
     7741 ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt ttttgatatc
     7801 gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt ctagatttcc
     7861 agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat cgactaaagc
     7921 ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt
     7981 cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg
     8041 aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga taacgacatc
     8101 tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa ctcgagtatg
     8161 gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc ttcgttctag
     8221 atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta cttccggata
     8281 gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg cttttggcgg
     8341 tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga tcattcgggt
     8401 agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa gaggtgttaa
     8461 ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa cgctttccac
     8521 gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc ctgccacttt
     8581 gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga tatcttctgc
     8641 acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa
     8701 tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca cagctagaga
     8761 taattccttt ttattcatct tactgtaata aggaatttta aattctttgg cataactata
     8821 aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc tcattcagct
     8881 cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc gataatatga
     8941 tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc tgccaatagt
     9001 ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc tgtgccgtga
     9061 agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc agcgcccatt
     9121 cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat gatttctgaa
     9181 gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt ggcaaatcct
     9241 ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta aacttcgatt
     9301 tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat aaagctttcc
     9361 aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc tgctaatgct
     9421 agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc atgtaatgtt
     9481 tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc ccccatatta
     9541 ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc aattactgtc
     9601 tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac tgaaaccacg
     9661 tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata catggcacca
     9721 tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag gtcaatcggt
     9781 ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa tttaccaagc
     9841 agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg cggcatcgga
     9901 atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg gacagttgcg
     9961 cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg cacaccttcc
    10021 aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc actatttttt
    10081 gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac gatttttttc
    10141 atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt ttatcataga
    10201 taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc
    10261 aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac gctttgttag
    10321 ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct ttacctggag
    10381 ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct tcgatatatt
    10441 tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact tttgagatac
    10501 ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca tgtaatacta
    10561 aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt
    10621 tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag tagtcgatac
    10681 ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta ccgatgattc
    10741 cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct tttgcatgtg
    10801 ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga tgtgaaccat
    10861 cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg taatcaccat
    10921 ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa tcagtgatta
    10981 aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat gtttggataa
    11041 gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat tccaagttgt
    11101 ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa aattcagctc
    11161 ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat ttgttaagac
    11221 aattcttaac caacgaccct attttaccaa acatttcggt agttttctac taaaatcaat
    11281 gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata gcgctttaat
    11341 cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat cagaaaaaga
    11401 gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt tctatcaagt
    11461 tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct ttgattaatt
    11521 tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc acaaagaatt
    11581 ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca cctgaaaaga
    11641 ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag cggtgacggt
    11701 gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt ccttttttga
    11761 ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg ttttcctgat
    11821 cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct gatgacgcat
    11881 cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata ccaagacaaa
    11941 tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt ttcccttcta
    12001 ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc aaacgttctt
    12061 ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg tctgaattat
    12121 aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct ggtaactcga
    12181 catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca cgttcttcta
    12241 attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg tcgcaaacaa
    12301 tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta tctacatctg
    12361 gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg tttttcacgc
    12421 tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata cctaaaccac
    12481 gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca gctttcaagt
    12541 aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct gctttcattt
    12601 gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc cctacttctg
    12661 taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct ttgatctcat
    12721 ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta cgttctttac
    12781 gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc aaattgatgt
    12841 caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca tctgttacga
    12901 atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg tatggatcaa
    12961 atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa gaagctgcaa
    13021 caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat tttgtcataa
    13081 gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa acaaagctcc
    13141 ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc tctaaaaggg
    13201 tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa tgaatactat
    13261 ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa gtcctttatg
    13321 agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta acgttaaacc
    13381 taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat aaaaaaataa
    13441 aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc ctacttgcag
    13501 caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta tttacctcct
    13561 ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc tattaaataa
    13621 tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt caacaaaagt
    13681 gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag gagatgcgac
    13741 aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc ctcggtcaca
    13801 agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg tgattcggag
    13861 ttaaatggta ggctcacaac ctctggataa acggt
//
LOCUS       DADOZM010000072        13864 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.073, whole genome shotgun sequence.
ACCESSION   DADOZM010000072 DADOZM010000000
VERSION     DADOZM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.073"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            385..1518
                     /locus_tag="J8I47_001750"
     CDS             385..1518
                     /locus_tag="J8I47_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAZ6323185.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1549..1869
                     /locus_tag="J8I47_001751"
     CDS             1549..1869
                     /locus_tag="J8I47_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323186.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1898..2362)
                     /locus_tag="J8I47_001752"
     CDS             complement(1898..2362)
                     /locus_tag="J8I47_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323187.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2494..3747)
                     /locus_tag="J8I47_001753"
     CDS             complement(2494..3747)
                     /locus_tag="J8I47_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323188.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3776..4093)
                     /locus_tag="J8I47_001754"
     CDS             complement(3776..4093)
                     /locus_tag="J8I47_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ6323189.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(4119..4439)
                     /locus_tag="J8I47_001755"
     CDS             complement(4119..4439)
                     /locus_tag="J8I47_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323190.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(4903..6318)
                     /locus_tag="J8I47_001756"
     CDS             complement(4903..6318)
                     /locus_tag="J8I47_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6323191.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(6335..7651)
                     /locus_tag="J8I47_001757"
     CDS             complement(6335..7651)
                     /locus_tag="J8I47_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323192.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(7801..8499)
                     /locus_tag="J8I47_001758"
     CDS             complement(7801..8499)
                     /locus_tag="J8I47_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAZ6323193.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(8788..9777)
                     /locus_tag="J8I47_001759"
     CDS             complement(8788..9777)
                     /locus_tag="J8I47_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAZ6323194.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(9857..10690)
                     /gene="msrA"
                     /locus_tag="J8I47_001760"
     CDS             complement(9857..10690)
                     /gene="msrA"
                     /locus_tag="J8I47_001760"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ6323195.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(10865..12499)
                     /locus_tag="J8I47_001761"
     CDS             complement(10865..12499)
                     /locus_tag="J8I47_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323196.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(12486..13856)
                     /locus_tag="J8I47_001762"
     CDS             complement(12486..13856)
                     /locus_tag="J8I47_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ6323197.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
BASE COUNT         4319 a         2767 c         2266 g         4512 t
ORIGIN      
        1 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
       61 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
      121 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
      181 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
      241 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
      301 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
      361 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
      421 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
      481 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
      541 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
      601 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
      661 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
      721 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
      781 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
      841 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
      901 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
      961 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     1021 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     1081 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     1141 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     1201 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     1261 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     1321 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     1381 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     1441 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     1501 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     1561 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     1621 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     1681 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     1741 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     1801 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     1861 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     1921 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     1981 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     2041 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     2101 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     2161 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     2221 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     2281 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     2341 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     2401 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     2461 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     2521 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     2581 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     2641 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     2701 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     2761 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     2821 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     2881 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     2941 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     3001 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     3061 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     3121 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     3181 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     3241 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     3301 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     3361 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     3421 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     3481 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     3541 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     3601 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     3661 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     3721 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     3781 cgttcaatcg acgatacaga ttaatgatct cttttgctaa atctgtaaaa gctatagatg
     3841 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     3901 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     3961 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     4021 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     4081 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     4141 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     4201 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     4261 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     4321 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     4381 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     4441 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     4501 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     4561 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     4621 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     4681 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     4741 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     4801 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
     4861 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     4921 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
     4981 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
     5041 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
     5101 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
     5161 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
     5221 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
     5281 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
     5341 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
     5401 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
     5461 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
     5521 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
     5581 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
     5641 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
     5701 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
     5761 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
     5821 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
     5881 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
     5941 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
     6001 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
     6061 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
     6121 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
     6181 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
     6241 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
     6301 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
     6361 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
     6421 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
     6481 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
     6541 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
     6601 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
     6661 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
     6721 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
     6781 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
     6841 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
     6901 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
     6961 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
     7021 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
     7081 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
     7141 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
     7201 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
     7261 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
     7321 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
     7381 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
     7441 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
     7501 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
     7561 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
     7621 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
     7681 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
     7741 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
     7801 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
     7861 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
     7921 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
     7981 agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc
     8041 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
     8101 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
     8161 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
     8221 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
     8281 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
     8341 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
     8401 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
     8461 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
     8521 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac
     8581 aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
     8641 taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat
     8701 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
     8761 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
     8821 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
     8881 acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat
     8941 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
     9001 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
     9061 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
     9121 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
     9181 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
     9241 ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt
     9301 tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc
     9361 tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc
     9421 acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg
     9481 ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa
     9541 aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc
     9601 gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg
     9661 atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga
     9721 atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt
     9781 ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca
     9841 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
     9901 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg
     9961 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    10021 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    10081 gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga tattgcttcc
    10141 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    10201 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    10261 ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc
    10321 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    10381 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    10441 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    10501 caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact
    10561 gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt
    10621 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    10681 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    10741 tatcataaag tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa
    10801 agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata aatgaaaagt
    10861 taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca gttaatgtaa
    10921 caagataaga aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc
    10981 ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat
    11041 gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct
    11101 tctcattagt tactctatat atgatagaac taccagttgt tttataagga aggtttaccc
    11161 attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga
    11221 taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt
    11281 ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac
    11341 tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg
    11401 tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta
    11461 tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa
    11521 gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct
    11581 ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac
    11641 caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga
    11701 acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga
    11761 gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg
    11821 cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca
    11881 aacgaataaa aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc
    11941 tgtacactag caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc
    12001 cagagatgat ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt
    12061 cggcaattgc ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac
    12121 aactaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta
    12181 cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt
    12241 ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta
    12301 caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata
    12361 accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat
    12421 ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc aatattacta
    12481 cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct
    12541 ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga
    12601 atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta
    12661 ttttctccag catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg
    12721 actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt
    12781 tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct
    12841 ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc
    12901 gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt
    12961 gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct
    13021 tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa
    13081 tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag
    13141 atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac
    13201 aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg
    13261 attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat
    13321 cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct
    13381 gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa
    13441 ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca
    13501 gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac
    13561 aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga
    13621 gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat
    13681 atcaatccat aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca
    13741 attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta
    13801 aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa actaatcaga
    13861 atcg
//
LOCUS       DADOZM010000073        13765 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.074, whole genome shotgun sequence.
ACCESSION   DADOZM010000073 DADOZM010000000
VERSION     DADOZM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.074"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            141..2612
                     /locus_tag="J8I47_001763"
     CDS             141..2612
                     /locus_tag="J8I47_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ6323198.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2715..4451)
                     /locus_tag="J8I47_001764"
     CDS             complement(2715..4451)
                     /locus_tag="J8I47_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAZ6323199.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4448..5152)
                     /locus_tag="J8I47_001765"
     CDS             complement(4448..5152)
                     /locus_tag="J8I47_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323200.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5283..5786
                     /locus_tag="J8I47_001766"
     CDS             5283..5786
                     /locus_tag="J8I47_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAZ6323201.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5746..6084
                     /locus_tag="J8I47_001767"
     CDS             5746..6084
                     /locus_tag="J8I47_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAZ6323202.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6105..7523
                     /gene="ffh"
                     /locus_tag="J8I47_001768"
     CDS             6105..7523
                     /gene="ffh"
                     /locus_tag="J8I47_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAZ6323203.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7635..8213
                     /locus_tag="J8I47_001769"
     CDS             7635..8213
                     /locus_tag="J8I47_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAZ6323204.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8217..8738
                     /locus_tag="J8I47_001770"
     CDS             8217..8738
                     /locus_tag="J8I47_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ6323205.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8817..9371)
                     /locus_tag="J8I47_001771"
     CDS             complement(8817..9371)
                     /locus_tag="J8I47_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ6323206.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9624..9899
                     /gene="rpsP"
                     /locus_tag="J8I47_001772"
     CDS             9624..9899
                     /gene="rpsP"
                     /locus_tag="J8I47_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAZ6323207.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9911..10162
                     /locus_tag="J8I47_001773"
     CDS             9911..10162
                     /locus_tag="J8I47_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAZ6323208.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10287..11078
                     /locus_tag="J8I47_001774"
     CDS             10287..11078
                     /locus_tag="J8I47_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323209.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11248..11769
                     /gene="rimM"
                     /locus_tag="J8I47_001775"
     CDS             11248..11769
                     /gene="rimM"
                     /locus_tag="J8I47_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAZ6323210.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11759..12520
                     /gene="trmD"
                     /locus_tag="J8I47_001776"
     CDS             11759..12520
                     /gene="trmD"
                     /locus_tag="J8I47_001776"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAZ6323211.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12656..13003
                     /gene="rplS"
                     /locus_tag="J8I47_001777"
     CDS             12656..13003
                     /gene="rplS"
                     /locus_tag="J8I47_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAZ6323212.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13135..>13765)
                     /locus_tag="J8I47_001778"
     CDS             complement(13135..>13765)
                     /locus_tag="J8I47_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323213.1"
                     /translation="KRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTG
                     MRPGEAIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPK
                     KAIAIYNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHT
                     HISKLAELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4511 a         2509 c         2816 g         3929 t
ORIGIN      
        1 tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag
       61 gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg
      121 aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag
      181 taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa
      241 cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca
      301 cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct
      361 ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg acttttttac
      421 cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct
      481 tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac gggatagaac
      541 aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag actggatatc
      601 aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag gctttattga
      661 caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc ggcagtttca
      721 ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc
      781 agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc
      841 ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt
      901 ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag
      961 cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa
     1021 gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat
     1081 tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt
     1141 ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg
     1201 aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg
     1261 ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt
     1321 ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg
     1381 aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa
     1441 aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga
     1501 cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa
     1561 gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata
     1621 atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg
     1681 ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt
     1741 ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata
     1801 cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca
     1861 gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg
     1921 attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata
     1981 ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt
     2041 tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt
     2101 tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca
     2161 gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga
     2221 tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt
     2281 ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt
     2341 caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt
     2401 cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc
     2461 tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag
     2521 aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa
     2581 aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa
     2641 agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga
     2701 gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga
     2761 tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac
     2821 cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca
     2881 aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat
     2941 agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca
     3001 gcattttcaa ttagattttt gataattgga taaaggatat ctgttttagt agtaaatggt
     3061 tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt
     3121 tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg
     3181 ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt
     3241 tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa
     3301 gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca
     3361 acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct
     3421 agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc
     3481 cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga
     3541 atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag
     3601 cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca
     3661 tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct
     3721 gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga
     3781 tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc
     3841 accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga
     3901 taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg
     3961 cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct
     4021 tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta
     4081 gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga
     4141 cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta
     4201 gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg
     4261 ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt
     4321 gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc
     4381 cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt
     4441 ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt
     4501 actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta
     4561 cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt
     4621 cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta
     4681 gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc
     4741 caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt tcaattcgtc
     4801 tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt
     4861 cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca
     4921 tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca
     4981 tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta
     5041 atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg
     5101 tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct
     5161 cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt
     5221 tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt
     5281 caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa
     5341 aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag
     5401 aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt ggaaatggat
     5461 tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag
     5521 cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag
     5581 tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt
     5641 ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga
     5701 ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag
     5761 accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg
     5821 aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac
     5881 gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat
     5941 tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat
     6001 atgactgcat atattacaga taagtacccg gaagataaaa aactacttga atatgtacag
     6061 caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct
     6121 taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag
     6181 ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact
     6241 tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc
     6301 tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca
     6361 cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga
     6421 tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa
     6481 agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga
     6541 tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg
     6601 aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg
     6661 actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt
     6721 tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga
     6781 ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg
     6841 tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag
     6901 tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat
     6961 tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg
     7021 aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg
     7081 aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc
     7141 cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg
     7201 tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag
     7261 cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt
     7321 ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa
     7381 tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt
     7441 caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga
     7501 agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc
     7561 tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg
     7621 aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc
     7681 atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc
     7741 ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg
     7801 atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca
     7861 gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc
     7921 aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt
     7981 ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa
     8041 caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta
     8101 tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg
     8161 acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc
     8221 gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg
     8281 atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg
     8341 aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg
     8401 agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac
     8461 aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa
     8521 tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg
     8581 aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga
     8641 ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat
     8701 tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc
     8761 aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac
     8821 tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat
     8881 gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc gatatatgct
     8941 tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt
     9001 gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc
     9061 caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc
     9121 acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca
     9181 cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat
     9241 ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa
     9301 gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt
     9361 ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac aaaagataag
     9421 taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt
     9481 tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc
     9541 tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa
     9601 aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta
     9661 aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt
     9721 tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag
     9781 aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca
     9841 tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag
     9901 gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac
     9961 ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct
    10021 aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc
    10081 aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat
    10141 catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt
    10201 ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc
    10261 ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc
    10321 tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca
    10381 gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg
    10441 aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac
    10501 aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg
    10561 cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg
    10621 ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg
    10681 ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg
    10741 gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa
    10801 gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag
    10861 gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac
    10921 cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg
    10981 tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt
    11041 ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc
    11101 catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa
    11161 ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa
    11221 cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta
    11281 aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa
    11341 cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta
    11401 acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct
    11461 tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct
    11521 gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa
    11581 gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg
    11641 gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa
    11701 gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat
    11761 gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat
    11821 catcggtaag gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta
    11881 ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct
    11941 gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt
    12001 caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga
    12061 agaattttca caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga
    12121 acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg
    12181 agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg
    12241 gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata
    12301 tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca
    12361 taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc
    12421 tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa
    12481 gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt
    12541 acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg
    12601 atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa
    12661 tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc
    12721 tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt
    12781 atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg
    12841 taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc
    12901 aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc
    12961 attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt tatcagactg
    13021 agaaagaacc cttggtattc aagggttctt tttttgttct ctgttttttt atattaacga
    13081 tttctatttg ggacattttt gattaataaa aaggggcaaa aaaggggcaa caagttacag
    13141 tttttctata tcttctttca atttttcttt tactcccttt gtcacatgca aataaatctt
    13201 ttcagtgata tcactatttt cgtgcccaac gcgatcctga atagcataca gaggagttcc
    13261 tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa gttttttatc
    13321 aatcttcatg tcagctttat gatttctcaa ataggtgtta attgctgtta gttggaaagg
    13381 agttcctttc gttgtttgaa atagaaattg accattcgga tttaattcta gaagctcatt
    13441 gtaaatagct atcgcttttt ttggtaaatc aatttctcgc attccagcag cagtttttgt
    13501 agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg tcccatttat
    13561 aactactgat gcattattat tggtaatgtg tacatcgtct ttacttaatg cgattgcttc
    13621 tccaggcctc attccagtca aatatagcca ctggaaaagg agagaatatc ttttattgtg
    13681 tgaagttgtg aaatttacta gtcttttata ttcatcatct tctaaaaact tatctttaat
    13741 ttttatcgtt tttgactctc gttta
//
LOCUS       DADOZM010000074        13489 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.075, whole genome shotgun sequence.
ACCESSION   DADOZM010000074 DADOZM010000000
VERSION     DADOZM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.075"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(334..894)
                     /locus_tag="J8I47_001779"
     CDS             complement(334..894)
                     /locus_tag="J8I47_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323214.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="J8I47_001780"
     CDS             1036..1650
                     /locus_tag="J8I47_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323215.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="J8I47_001781"
     CDS             1839..3719
                     /locus_tag="J8I47_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAZ6323216.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="J8I47_001782"
     CDS             3798..4025
                     /locus_tag="J8I47_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAZ6323217.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="J8I47_001783"
     CDS             4030..4572
                     /locus_tag="J8I47_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ6323218.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="J8I47_001784"
     CDS             4799..5515
                     /locus_tag="J8I47_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAZ6323219.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7593)
                     /locus_tag="J8I47_001785"
     CDS             complement(5575..7593)
                     /locus_tag="J8I47_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAZ6323220.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="J8I47_001786"
     CDS             complement(7606..8604)
                     /locus_tag="J8I47_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323221.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8802..10043
                     /gene="sstT"
                     /locus_tag="J8I47_001787"
     CDS             8802..10043
                     /gene="sstT"
                     /locus_tag="J8I47_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAZ6323222.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="J8I47_001788"
     CDS             10190..10825
                     /locus_tag="J8I47_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAZ6323223.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="J8I47_001789"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="J8I47_001789"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAZ6323224.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..13280)
                     /locus_tag="J8I47_001790"
     CDS             complement(11901..13280)
                     /locus_tag="J8I47_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ6323225.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         4516 a         2133 c         2629 g         4211 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgccaggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt
    13261 aaaacaagat ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga
    13321 taaaaaatat tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc
    13381 catacattat ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta
    13441 tgatagattc ccttagacat gaatagaggg atgacgtaag caaaactta
//
LOCUS       DADOZM010000075        13461 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.076, whole genome shotgun sequence.
ACCESSION   DADOZM010000075 DADOZM010000000
VERSION     DADOZM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.076"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            112..279
                     /locus_tag="J8I47_001791"
     CDS             112..279
                     /locus_tag="J8I47_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAZ6323226.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(341..2152)
                     /locus_tag="J8I47_001792"
     CDS             complement(341..2152)
                     /locus_tag="J8I47_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAZ6323227.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2149..2514)
                     /locus_tag="J8I47_001793"
     CDS             complement(2149..2514)
                     /locus_tag="J8I47_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAZ6323228.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2677..3072)
                     /locus_tag="J8I47_001794"
     CDS             complement(2677..3072)
                     /locus_tag="J8I47_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ6323229.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3090..4052)
                     /locus_tag="J8I47_001795"
     CDS             complement(3090..4052)
                     /locus_tag="J8I47_001795"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAZ6323230.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4052..4264)
                     /locus_tag="J8I47_001796"
     CDS             complement(4052..4264)
                     /locus_tag="J8I47_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAZ6323231.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4285..4983)
                     /locus_tag="J8I47_001797"
     CDS             complement(4285..4983)
                     /locus_tag="J8I47_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAZ6323232.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5003..5545)
                     /locus_tag="J8I47_001798"
     CDS             complement(5003..5545)
                     /locus_tag="J8I47_001798"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAZ6323233.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5677..6681)
                     /locus_tag="J8I47_001799"
     CDS             complement(5677..6681)
                     /locus_tag="J8I47_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAZ6323234.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6678..7667)
                     /locus_tag="J8I47_001800"
     CDS             complement(6678..7667)
                     /locus_tag="J8I47_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAZ6323235.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7664..8470)
                     /locus_tag="J8I47_001801"
     CDS             complement(7664..8470)
                     /locus_tag="J8I47_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323236.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8636..9592
                     /locus_tag="J8I47_001802"
     CDS             8636..9592
                     /locus_tag="J8I47_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323237.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9669..10187)
                     /locus_tag="J8I47_001803"
     CDS             complement(9669..10187)
                     /locus_tag="J8I47_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ6323238.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10275..10424)
                     /gene="rpmG"
                     /locus_tag="J8I47_001804"
     CDS             complement(10275..10424)
                     /gene="rpmG"
                     /locus_tag="J8I47_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ6323239.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10652..11098
                     /locus_tag="J8I47_001805"
     CDS             10652..11098
                     /locus_tag="J8I47_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAZ6323240.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11293..13188)
                     /gene="asnB"
                     /locus_tag="J8I47_001806"
     CDS             complement(11293..13188)
                     /gene="asnB"
                     /locus_tag="J8I47_001806"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAZ6323241.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4197 a         2846 c         2293 g         4125 t
ORIGIN      
        1 gtaaacattt tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
       61 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
      121 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
      181 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
      241 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
      301 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
      361 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
      421 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
      481 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
      541 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
      601 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
      661 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
      721 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
      781 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
      841 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
      901 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
      961 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
     1021 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
     1081 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
     1141 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
     1201 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
     1261 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
     1321 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
     1381 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
     1441 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
     1501 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
     1561 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
     1621 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
     1681 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
     1741 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
     1801 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
     1861 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
     1921 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
     1981 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
     2041 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
     2101 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
     2161 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
     2221 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
     2281 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
     2341 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
     2401 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
     2461 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
     2521 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
     2581 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
     2641 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
     2701 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
     2761 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
     2821 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
     2881 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
     2941 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
     3001 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
     3061 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
     3121 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
     3181 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
     3241 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
     3301 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
     3361 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
     3421 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
     3481 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
     3541 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
     3601 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
     3661 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
     3721 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
     3781 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
     3841 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
     3901 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
     3961 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
     4021 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
     4081 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
     4141 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
     4201 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
     4261 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
     4321 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
     4381 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
     4441 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
     4501 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
     4561 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
     4621 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
     4681 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
     4741 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
     4801 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
     4861 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
     4921 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
     4981 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
     5041 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
     5101 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
     5161 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
     5221 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
     5281 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
     5341 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
     5401 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
     5461 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
     5521 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
     5581 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
     5641 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
     5701 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
     5761 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
     5821 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
     5881 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
     5941 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
     6001 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
     6061 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
     6121 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
     6181 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
     6241 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
     6301 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
     6361 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
     6421 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
     6481 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
     6541 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
     6601 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
     6661 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
     6721 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
     6781 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
     6841 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
     6901 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
     6961 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
     7021 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
     7081 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
     7141 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
     7201 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
     7261 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
     7321 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
     7381 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcaccccttg catcaatgca
     7441 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
     7501 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
     7561 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
     7621 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
     7681 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
     7741 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
     7801 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
     7861 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
     7921 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
     7981 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
     8041 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
     8101 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
     8161 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
     8221 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
     8281 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
     8341 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
     8401 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
     8461 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
     8521 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
     8581 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
     8641 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
     8701 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
     8761 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
     8821 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
     8881 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
     8941 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
     9001 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
     9061 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
     9121 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
     9181 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
     9241 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
     9301 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
     9361 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
     9421 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
     9481 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
     9541 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
     9601 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
     9661 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
     9721 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
     9781 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
     9841 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
     9901 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
     9961 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    10021 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    10081 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    10141 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    10201 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    10261 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    10321 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    10381 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    10441 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    10501 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    10561 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    10621 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    10681 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    10741 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    10801 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    10861 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    10921 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    10981 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    11041 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    11101 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    11161 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    11221 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    11281 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    11341 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    11401 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    11461 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    11521 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    11581 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    11641 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    11701 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    11761 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    11821 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    11881 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    11941 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    12001 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    12061 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    12121 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    12181 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    12241 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    12301 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    12361 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    12421 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    12481 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    12541 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    12601 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    12661 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    12721 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    12781 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    12841 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    12901 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    12961 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    13021 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    13081 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    13141 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    13201 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    13261 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    13321 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    13381 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    13441 gacacgacct aaagatcgtg t
//
LOCUS       DADOZM010000076        13400 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.077, whole genome shotgun sequence.
ACCESSION   DADOZM010000076 DADOZM010000000
VERSION     DADOZM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.077"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(16..708)
                     /locus_tag="J8I47_001807"
     CDS             complement(16..708)
                     /locus_tag="J8I47_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ6323242.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="J8I47_001808"
                     /pseudo
     CDS             793..969
                     /locus_tag="J8I47_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="J8I47_001809"
     CDS             complement(1145..1714)
                     /locus_tag="J8I47_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ6323243.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="J8I47_001810"
     CDS             complement(1788..2111)
                     /locus_tag="J8I47_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323244.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="J8I47_001811"
     CDS             complement(2265..3077)
                     /locus_tag="J8I47_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323245.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="J8I47_001812"
     CDS             3431..4279
                     /locus_tag="J8I47_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAZ6323246.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="J8I47_001813"
     CDS             4424..5365
                     /locus_tag="J8I47_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAZ6323247.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="J8I47_001814"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="J8I47_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="J8I47_001815"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="J8I47_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAZ6323248.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="J8I47_001816"
     CDS             complement(9829..12015)
                     /locus_tag="J8I47_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAZ6323249.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="J8I47_001817"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="J8I47_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAZ6323250.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="J8I47_001818"
     CDS             complement(12237..12677)
                     /locus_tag="J8I47_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAZ6323251.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="J8I47_001819"
     CDS             complement(12751..13290)
                     /locus_tag="J8I47_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6323252.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3766 a         2807 c         2381 g         4446 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaaagtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtatg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtatgatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcagca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt tgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agtcacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttagatttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagcacaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gataataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa
//
LOCUS       DADOZM010000077        13212 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.080, whole genome shotgun sequence.
ACCESSION   DADOZM010000077 DADOZM010000000
VERSION     DADOZM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.080"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(8..1210)
                     /locus_tag="J8I47_001820"
     CDS             complement(8..1210)
                     /locus_tag="J8I47_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6323253.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1327..1842
                     /locus_tag="J8I47_001821"
     CDS             1327..1842
                     /locus_tag="J8I47_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ6323254.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2041..2487)
                     /locus_tag="J8I47_001822"
     CDS             complement(2041..2487)
                     /locus_tag="J8I47_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAZ6323255.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2534..4012)
                     /gene="msr"
                     /locus_tag="J8I47_001823"
     CDS             complement(2534..4012)
                     /gene="msr"
                     /locus_tag="J8I47_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ6323256.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4366..4995)
                     /locus_tag="J8I47_001824"
     CDS             complement(4366..4995)
                     /locus_tag="J8I47_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ6323257.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(4979..5443)
                     /locus_tag="J8I47_001825"
     CDS             complement(4979..5443)
                     /locus_tag="J8I47_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6323258.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5930..7198)
                     /locus_tag="J8I47_001826"
     CDS             complement(5930..7198)
                     /locus_tag="J8I47_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAZ6323259.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7429..8166)
                     /locus_tag="J8I47_001827"
     CDS             complement(7429..8166)
                     /locus_tag="J8I47_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6323260.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8233..8802)
                     /locus_tag="J8I47_001828"
     CDS             complement(8233..8802)
                     /locus_tag="J8I47_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAZ6323261.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8930..9802
                     /locus_tag="J8I47_001829"
     CDS             8930..9802
                     /locus_tag="J8I47_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6323262.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10144..11016
                     /locus_tag="J8I47_001830"
     CDS             10144..11016
                     /locus_tag="J8I47_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6323263.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11013..12971
                     /locus_tag="J8I47_001831"
     CDS             11013..12971
                     /locus_tag="J8I47_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323264.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT         3970 a         2703 c         2427 g         4112 t
ORIGIN      
        1 tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg atacaacata
       61 cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca atatgttcac
      121 caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca aaactatagc
      181 tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc gtcaacagtg
      241 tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca aggactggca
      301 tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat gctgctaaaa
      361 ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag
      421 ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa tagccaatca
      481 ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac aatgcgatta
      541 gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa aggcctactt
      601 ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca
      661 tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag accccaccga
      721 ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg attgcttttt
      781 ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca aataaagcat
      841 tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca gataatccgc
      901 caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt ttcaaaccga
      961 tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac atgtaactag
     1021 taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc gtttttaaga
     1081 ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct acaactagcg
     1141 ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt tgattctcca
     1201 atatttccat ccctttctct ctattagcat catacatgat ttattttaca tgtcaatatg
     1261 cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac gagaggtagg
     1321 atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa accacgttct
     1381 gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag tcagatctat
     1441 acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa agacgagcac
     1501 tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga ggaatggatc
     1561 aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt
     1621 tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca acagctgaac
     1681 aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa gaaacaagaa
     1741 tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga actccaatgg
     1801 tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa cagaaccttt
     1861 ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca gttggaaaag
     1921 aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt ttctagaact
     1981 gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa cagtgttcca
     2041 tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa cgatgatcaa
     2101 ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag cagagaataa
     2161 ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg tcaccattcc
     2221 taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg gatttactgg
     2281 tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca aaatgaaagc
     2341 caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat cttgatgcag
     2401 ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg ttgtaaaaat
     2461 tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg tcacgaaaat
     2521 catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc ccttttattt
     2581 cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta aacaaaatcg
     2641 ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata tcaataaaat
     2701 tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa gcaatcgtca
     2761 gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa
     2821 accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt tgtctcatga
     2881 caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga tatacttgaa
     2941 agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt tctaataagg
     3001 ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa
     3061 aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt tgtcccatga
     3121 tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt tgtttcatct
     3181 gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt ttcgcattct
     3241 tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga tccagtctga
     3301 tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt
     3361 cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct
     3421 gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc
     3481 ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat
     3541 catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa
     3601 tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag
     3661 aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag
     3721 tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag
     3781 cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat
     3841 cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc
     3901 ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct
     3961 ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta
     4021 cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt
     4081 tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac
     4141 caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc
     4201 catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga
     4261 aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca
     4321 cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacatagt
     4381 gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga
     4441 taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc
     4501 tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa
     4561 ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa
     4621 actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt
     4681 ccctgattcg taagcttggt tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc
     4741 aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg
     4801 acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg gtttcatatt
     4861 gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat
     4921 tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc
     4981 atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg
     5041 tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg
     5101 atagcacgga acctgtatct ttatggtcca tttcacaatg cttgattttc ttctgggctt
     5161 tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat
     5221 agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact
     5281 tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt
     5341 atctagtaag acttataccg atattttctt caaagatctg gacgattttt tgatcggcta
     5401 atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa
     5461 taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag
     5521 aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa agaaattttt
     5581 tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag attattgatt
     5641 cttatcaaac tatagaagat agctcttctg cagtagataa ttttacaccg ccaaaaattt
     5701 ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag tatgcttaaa
     5761 cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa acggtgttca
     5821 aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat ctgtttttgt
     5881 ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt taataaccat
     5941 aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt tccttagcac
     6001 tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga taaattggct
     6061 tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt gttaaagagt
     6121 tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg tataaacggt
     6181 agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc cagcctacag
     6241 ttggcagata atttttttct ccggcattca atgtatataa atgttctcct gtatgacggt
     6301 tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt gtcagattat
     6361 aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca taacgacctg
     6421 ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca ccggaatagt
     6481 agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct tgcaatgatt
     6541 ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc ccattcacat
     6601 attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc ccaaatagat
     6661 tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg atggcttgag
     6721 caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca gttgaggcaa
     6781 tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa gcatagccag
     6841 tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg
     6901 tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct gctgatgtac
     6961 tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt gaaattgtat
     7021 tttctgagac agtagctgac tgcaaagtac tttctgtatc gttttcaatt cctgtttctg
     7081 aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc actgttgtgt
     7141 tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt gctttcatct
     7201 tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa aaagtaaaag
     7261 caagtcaatg tccatattta aataacattt gtttcttttt aataaatttg ctttttctgc
     7321 ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt
     7381 gaataactat ttaacaagta attctctctg gatattatcc gttatggatt atacttcctg
     7441 tgtcgtcgga cggataatca tgttgctgac agacatccgg tctggcgttt ctatcgcaaa
     7501 tgctacagca gaagctatat cttcagatgt taatccccat tctttttgcg cttcatgaag
     7561 ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg
     7621 agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat
     7681 tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc
     7741 cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac
     7801 ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc
     7861 acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc
     7921 gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct
     7981 tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag
     8041 gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt
     8101 tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc
     8161 attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt
     8221 tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc
     8281 aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt
     8341 ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt
     8401 gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac
     8461 cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa
     8521 gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc
     8581 gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat
     8641 attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa
     8701 aggctcttcg ttttccgctt ggatctgatg gatcacttca aagagcggat ccgttactcg
     8761 aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat
     8821 ccttacttta taacggacgg cttatgatgt ctaatactta taaaacatac ttaactattc
     8881 cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg
     8941 tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc
     9001 tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg
     9061 tgtgacactt ttcactcgag gctcccgaga gatacagttg acggattctg gtcgatatct
     9121 ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa
     9181 tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt
     9241 catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata
     9301 tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg attttggact
     9361 cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg
     9421 ttggggactt ttgatacatc ggaatcatcc tttagcaaag caaacagtca tccatccgca
     9481 ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc
     9541 tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta
     9601 caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat
     9661 caatacttat gggacggaaa aagtattcat cccactcgat ccgccattac aagcttcact
     9721 gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt
     9781 cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac
     9841 gaataagtta agcaaaagat ccaatgatta aataacataa gtttcttaat ttgttccttc
     9901 cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca
     9961 taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata
    10021 ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt
    10081 tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg
    10141 cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag
    10201 gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt
    10261 gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat
    10321 ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg
    10381 gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt
    10441 gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa
    10501 atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca
    10561 aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac
    10621 agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct
    10681 gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga accattttca
    10741 ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa
    10801 caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc
    10861 tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg
    10921 aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa
    10981 acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac
    11041 gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa
    11101 actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg
    11161 cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg
    11221 acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt
    11281 taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt
    11341 ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt
    11401 ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc
    11461 tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat
    11521 ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg
    11581 gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag
    11641 caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg
    11701 cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga
    11761 tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag
    11821 caaaaacagc ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag
    11881 aaccacttga attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt
    11941 tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta
    12001 gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga
    12061 tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta
    12121 tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata
    12181 ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat
    12241 taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag
    12301 tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga
    12361 agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag
    12421 atgagatggc aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg
    12481 ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg
    12541 ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg
    12601 acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca
    12661 caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct
    12721 tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa
    12781 aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc
    12841 aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa
    12901 gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca
    12961 acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag
    13021 acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg
    13081 cggtaataag tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta
    13141 ttacttgatg cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt
    13201 atcggtatga ag
//
LOCUS       DADOZM010000078        13211 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.081, whole genome shotgun sequence.
ACCESSION   DADOZM010000078 DADOZM010000000
VERSION     DADOZM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.081"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            147..1016
                     /locus_tag="J8I47_001832"
     CDS             147..1016
                     /locus_tag="J8I47_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAZ6323265.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="J8I47_001833"
     CDS             1225..2250
                     /locus_tag="J8I47_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAZ6323266.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="J8I47_001834"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="J8I47_001834"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAZ6323267.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="J8I47_001835"
     CDS             3333..5036
                     /locus_tag="J8I47_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323268.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="J8I47_001836"
     CDS             5038..7071
                     /locus_tag="J8I47_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ6323269.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="J8I47_001837"
     CDS             7082..7522
                     /locus_tag="J8I47_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323270.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8684)
                     /locus_tag="J8I47_001838"
     CDS             complement(7722..8684)
                     /locus_tag="J8I47_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323271.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8851..9528)
                     /locus_tag="J8I47_001839"
     CDS             complement(8851..9528)
                     /locus_tag="J8I47_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323272.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9792..11558
                     /locus_tag="J8I47_001840"
     CDS             9792..11558
                     /locus_tag="J8I47_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAZ6323273.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11573..12010
                     /locus_tag="J8I47_001841"
     CDS             11573..12010
                     /locus_tag="J8I47_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323274.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12023..13174
                     /locus_tag="J8I47_001842"
     CDS             12023..13174
                     /locus_tag="J8I47_001842"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAZ6323275.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4487 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaagtggga
     8761 atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga
     8821 gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc
     8881 taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc
     8941 tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc
     9001 ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc
     9061 gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa
     9121 gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat
     9181 cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa
     9241 agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa
     9301 gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc
     9361 ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt
     9421 cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac
     9481 acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc
     9541 aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt
     9601 tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct
     9661 gataccgaaa tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc
     9721 aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag
     9781 ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac
     9841 aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct
     9901 ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag gctttggcta
     9961 ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa
    10021 aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg
    10081 tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg
    10141 ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag
    10201 tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga
    10261 ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat
    10321 ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga
    10381 ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg
    10441 gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg
    10501 cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc
    10561 actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat
    10621 tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg
    10681 gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca
    10741 attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt
    10801 ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc
    10861 aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta
    10921 cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga
    10981 tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca
    11041 atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac
    11101 tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt
    11161 tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag
    11221 tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca
    11281 aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc
    11341 tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag
    11401 atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac
    11461 agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat
    11521 acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag
    11581 tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg
    11641 ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat
    11701 ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg
    11761 aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga
    11821 tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc
    11881 aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat
    11941 ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc
    12001 aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg
    12061 aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa
    12121 tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga
    12181 atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga
    12241 aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat
    12301 cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag
    12361 cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga
    12421 aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc
    12481 aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca
    12541 agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca
    12601 attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag
    12661 tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat
    12721 tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac
    12781 aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga
    12841 aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg
    12901 gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca
    12961 agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta
    13021 tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga
    13081 acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca
    13141 aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg
    13201 acgaaatttt t
//
LOCUS       DADOZM010000079        13095 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.082, whole genome shotgun sequence.
ACCESSION   DADOZM010000079 DADOZM010000000
VERSION     DADOZM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.082"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(264..2390)
                     /gene="pnp"
                     /locus_tag="J8I47_001843"
     CDS             complement(264..2390)
                     /gene="pnp"
                     /locus_tag="J8I47_001843"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAZ6323276.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="J8I47_001844"
     CDS             complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="J8I47_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAZ6323277.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            3098..3661
                     /locus_tag="J8I47_001845"
     CDS             3098..3661
                     /locus_tag="J8I47_001845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAZ6323278.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3914..4435)
                     /locus_tag="J8I47_001846"
     CDS             complement(3914..4435)
                     /locus_tag="J8I47_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAZ6323279.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4438..5280)
                     /locus_tag="J8I47_001847"
     CDS             complement(4438..5280)
                     /locus_tag="J8I47_001847"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAZ6323280.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5273..5998)
                     /locus_tag="J8I47_001848"
     CDS             complement(5273..5998)
                     /locus_tag="J8I47_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAZ6323281.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6174..6470
                     /locus_tag="J8I47_001849"
     CDS             6174..6470
                     /locus_tag="J8I47_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323282.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            7038..8630
                     /locus_tag="J8I47_001850"
     CDS             7038..8630
                     /locus_tag="J8I47_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ6323283.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8643..9383
                     /locus_tag="J8I47_001851"
     CDS             8643..9383
                     /locus_tag="J8I47_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323284.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9551..10900
                     /locus_tag="J8I47_001852"
     CDS             9551..10900
                     /locus_tag="J8I47_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323285.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11028..11618)
                     /locus_tag="J8I47_001853"
     CDS             complement(11028..11618)
                     /locus_tag="J8I47_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAZ6323286.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11854..12933
                     /locus_tag="J8I47_001854"
     CDS             11854..12933
                     /locus_tag="J8I47_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6323287.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         3680 a         2714 c         2681 g         4020 t
ORIGIN      
        1 cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct gttctgcatc
       61 aagtaataag aacagccaaa taaaaacagc tcctaatgtt tttaacttgg ctggacttat
      121 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg tgacacggct
      181 tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc acgatcacaa
      241 gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc atcttcgttc
      301 aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt tactttcaca
      361 aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc gtttgctaat
      421 tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc accgaatttt
      481 tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt ggtcaaatct
      541 tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat acttactttt
      601 ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt gatcgtatcg
      661 ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat ttcgattttt
      721 ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa ttcttctaag
      781 atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg ttctgtgatt
      841 ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc ggcaacttta
      901 aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat cgtatagttt
      961 tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat tggtacacct
     1021 gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga accatttgat
     1081 tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga agggattact
     1141 tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc tggagagcct
     1201 gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat gaatcgtttg
     1261 ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc gagtgtacaa
     1321 gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac acgtggcaat
     1381 aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc aggacggatc
     1441 ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt gatttgttta
     1501 acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc ataagcttcg
     1561 cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc ttcagtcatt
     1621 actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa tacttcatca
     1681 acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat ttcttcttgg
     1741 aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag catgtcttct
     1801 tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt tccggcaaca
     1861 gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac gtactcgcca
     1921 tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc tgagattgat
     1981 agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt ttctacactc
     2041 atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat cgggcgaatt
     2101 ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc ttctcgtttg
     2161 atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt gatcgttaat
     2221 gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa gactactgta
     2281 tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc gacttcgatt
     2341 tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat aacttctcct
     2401 tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa agccgtcttt
     2461 tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa ttttacttaa
     2521 ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg actaaaatta
     2581 acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag ttttacgtaa
     2641 gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt atgaatgatg
     2701 gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag ttaatacagc
     2761 gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt tgatgatttc
     2821 attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt cccctgcgac
     2881 tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta gagcacacag
     2941 ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga aaaggtagtt
     3001 ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc gaaaatagac
     3061 ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg atgatatcat
     3121 tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc cattaagcga
     3181 agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc aagatccaat
     3241 caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc aattagatat
     3301 ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag atggaccttc
     3361 tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag atgcttgttt
     3421 aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg ttgttcgcca
     3481 tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga ttcgtttgaa
     3541 gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg tgatgttcta
     3601 tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg tgatcgagta
     3661 gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa aaggggagtg
     3721 ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta gatagacatc
     3781 tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct tattttttct
     3841 tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag gataaaaagt
     3901 agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt tgtttgatgt
     3961 ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg cctgttaatt
     4021 cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg atatcttgtg
     4081 gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc acaattacat
     4141 caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt tgatcttttc
     4201 catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt tcgaggatat
     4261 aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca atatcgccta
     4321 caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc actggttttt
     4381 ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg atcacactta
     4441 ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa catacgtgtc
     4501 tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat ccaatacttg
     4561 ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt gtcctatttt
     4621 agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga tcatatcgat
     4681 cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg tcttgatgat
     4741 acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca caaccgaacc
     4801 tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa ctcctccgcc
     4861 taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc tgttacgttt
     4921 cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga acatgcagtt
     4981 atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg cataaaaatt
     5041 ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca tatagatttt
     5101 tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg ctgattctgc
     5161 ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca gctcaggatc
     5221 attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga attcagtcat
     5281 aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct tgaataaacg
     5341 tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg gctgacggct
     5401 ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca acaatgacga
     5461 tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct tgaaaccctt
     5521 caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt ttgtcgctgt
     5581 gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat tgatacagac
     5641 tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg tcgatgtttt
     5701 gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa tgaatgatat
     5761 tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta tccggttcat
     5821 tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg tagattcctg
     5881 acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg gtcaaaaatg
     5941 taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat tgtttcatgt
     6001 tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt gaaaagtcag
     6061 aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat gttcactgac
     6121 aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc gatatgttac
     6181 cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg ttaagcgttt
     6241 atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct tttgctttat
     6301 tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga acatggggct
     6361 ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat ggcgtcgttg
     6421 ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa atgatcgaga
     6481 tggaactgga cacaggaaat tttctgttct agttcttttt ttattgtatt acatacattt
     6541 ttgtgtataa ttatactatc gtttgcaatg gatagatgat tcacattttg attgtttttt
     6601 cggtaaaagt aaagatttct atatttatca gtattttttc aaaaaccagc agttatagat
     6661 tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa ataactgaat acgagatgcc
     6721 tcaatcaatg aggtagaggt cgcgagaatc attaaccctg tggagtgaag caaacatgga
     6781 gaagcaggaa tagggaggat cgccgaaatg tgcacttttt tgcttggagt gtatgttggg
     6841 acgcaagtta agagcttgcg gactgtctta gcagtgatgc taagttgcgc tatgttttgt
     6901 gagaggttta tcaccttatg gaattttagc ggccttttta tatctataaa tcggtcgctt
     6961 tttatttttt cagataaagc tttaccgcat ttcatctaga ccgctcgtgt atcatataat
     7021 cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt tgccgtacta tttgcttttg
     7081 tcttctcact attcagtgtg ggatctctgg caaatgcaga tgaaaaaaca ccgagcgatg
     7141 agtttcgcgt aggtatggag gctgggtatg cacccttcaa ctggtctcaa caaacagatg
     7201 agaataatgc cttgccaatt caaggccaaa atagttacgc cggcggttat gatgttcaaa
     7261 tcgctaaaaa agtagcagat ggtttaggga aaaaattagt catcgtccaa acgaaatggg
     7321 atggtctggc tcctgccctt caatcaggca agatcgacgc gatcatcgcc ggaatgagcc
     7381 ctactgctga acgacgaaaa gaaattgatt ttaccgatcc ttattacgaa tcacaattag
     7441 tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa aagtttagca gatctttctg
     7501 gagcaaagat cactgctcag ttaaatacat tccactatac tgtgatcgat cagataccag
     7561 atgtgcagaa acaacaagca atggataatt tctcagcaat gagaacagct ttagcttctg
     7621 gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt aacggcaact agtgtgaaca
     7681 aagacttgga aatgcttgaa ttcagcggtg cagatggctt ccagacaaac ccggaagatg
     7741 ttcagatttc tgttggtatg cgtaaaggcg acccggatat ccaaaaagta aaccagattc
     7801 tttctggcat ctctaacgat gaacgaacca aaattatgga ccaagcagtc aaagatcagc
     7861 ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc tgatttcact acgatttgga
     7921 aacaatacgg caatatgttc ttacgaggtg ctggacttac cttgttcatc gcattgatcg
     7981 ggacagttgt cggtactaca cttggattac tgattggtgt cttccgttcg gtacctgaat
     8041 caggaaatgc actgactcgc ttcttccaaa aaatcggaaa tgcacttctt tctatctatg
     8101 tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat ggtcatcttc tacggtctgg
     8161 cactcgcttt tggcatttca ttgaaccgaa cgattgccgc tttgtttatt gtctctctca
     8221 atactggagc ttacatgaca gagatcgttc gtggggggat ttttgcagta gatgaagggc
     8281 agttcgaagc tgcgcaagcc attggtatga cccatggcca aacgatgaga aaagttgtta
     8341 ttccacaagt tttacggaat atcttgccag caacaggaaa tgagtttgtt atcaatatca
     8401 aagatacagc agttttaagt gtaatcggcg tagctgattt gttcttccaa ggaaactctg
     8461 cttccggtgc gaatttccaa ttcttccaaa catttacgat cgtcgggatc atctacttag
     8521 tcatgacctt tacgatcacc agaatcctgc gggcggtaga aaagaaaatc gatggtcctt
     8581 cagcttatac caagcttgaa gaaatcgaca attcaaattt gcaggaataa ggaggaagaa
     8641 tcatgaatga aaaaattatt gaaatcaaac atctaagaaa aagcttcggt gaaaatgaag
     8701 tattgaaaga tatcagcgta acagtcaata aaggtgaagt tgtgacgatc atcggttctt
     8761 ccggttctgg taaatctact ttcttgcgct gtatcaattt actggaaaaa ccaacaggcg
     8821 gcgagatcat ttataatgga gaaaatgtat tagctcctaa atataattta cctaaatatc
     8881 gaacgaatct aggtatggtt ttccaatcct tcaacctgtt caacaatatg aatgtcttgg
     8941 aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga taaagaaact gccaaaaaag
     9001 tagcaatcga aaatctggaa aaagttggta tggatcgtta tatcgaagcc aaaccagcac
     9061 aattatctgg tggacagaaa cagcgtgtcg caattgctcg ggcattatca atgaatccag
     9121 atgtattgct attcgacgaa cctacttcgg cgttagaccc tgaaatggtt ggcgaagtgc
     9181 ttaatacgat caaagactta gcacatactg gcttgacaat gcttatcgtg acgcatgaga
     9241 tggaatttgc aaaagatgta tctgaccgtg tcatttttat ggataaaggt gtgattgcag
     9301 aagaaggtac accagaagat atctttgtcc atccgaaaga agaacgtaca aaagaattcc
     9361 tttcacgtat tttaaatgct taggatcaat agctgattgg gacaaagagc tgcggtgttt
     9421 accgcagttc ttttttatga attactcata aaaggtaaat ttctgaaaat tttggaatct
     9481 tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa ttaaactttt ttagttagga
     9541 tgatttaaaa atgaatgaag agcaagaact gcagcgtgga ctgaaaaata gacatgtcca
     9601 actgatttct attggcggag cgattggaac ggggttgttt ttaggttccg gaaagtctat
     9661 ccagctagct ggcccttcga tattgctcgc ctatcttatt actggatgca tctgtttttt
     9721 cattatgcgt gcgttaggcg aacttctgtt atcaaacacc aataaccatt cttttctgga
     9781 ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc actggctgga cgtattggtt
     9841 ctgctgggta tcgattgcaa tggccgattt aacagccatc ggaatgtatg tacgctactg
     9901 ggcgcctggt gttcctcagt ggcttcctga actgattgca ttaggtcttc ttctttgctt
     9961 gaatatggtg gctgtctcat tgtttggtga gttagagttt tggtttgcat tgatcaaagt
    10021 tgtagcgatc attgccttta tcctcgtagg agcttatatg atcctcaccc actatacgac
    10081 agatattggc acagccagta tcacgaatct atggtctcat ggcggattct ttccaactgg
    10141 tgcaaaagga tttatcttag ctttccaaat ggtaaccttt gcttttgctg ggatagaatt
    10201 agttggatta gtcgctggag aaacagaaaa tcctgaaaaa gtgttgccgg aagctatcaa
    10261 taatattcct atcaggatca tcttatttta cttaggatct ctttttgtca ttatggcaat
    10321 ctatccatgg aacagcttga atccagataa cagtccattt gtagaagtat tttcggaaat
    10381 tgggatcacg attgctgctt ccttgatcaa tttagtcgta ttaagtgcag cagcttctgc
    10441 ctgcaacagt gcaatatata gtacaggacg tatgcttcgt tctttagcgc aagaaggcag
    10501 tgcccctaaa aaattcaaaa agttaacaac acatcatgtg ccagggaatg ctttgacatt
    10561 ttctactatc gtcattttca tttctgttat cttgaattac gtgatgccta gtgaagtatt
    10621 cacactagta agcagtattg ccactacttg tttcttgttt atctggagta tgcttgtcta
    10681 cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa gctcattcat ttaaaatgcc
    10741 gttgtaccca tttagtaatt atctggtatt tctttacatg gcctttgtgt gtgtcgtctt
    10801 gttcctagga aaagatacga gaatcgcttt attactaaca cctgtgtggt tcttgcttct
    10861 attactaatc tatcatatga aatatgaacg taagaagtaa gaaagtagtt gtgacagaag
    10921 tggtcagctt cgagaaataa gaaggaattt acgaaaattg cttcttaaat ttttgtgaaa
    10981 tttcggctta tttcttggag ataaaagctt ctgtcccagc ctcttttcta tttccccgga
    11041 aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat accagataca
    11101 aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat caagcataag
    11161 ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac gacatgggaa
    11221 tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa actccacatc
    11281 gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg cccgacccaa
    11341 ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga ccagaccact
    11401 cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca gatcaccgca
    11461 acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca taaagaatga
    11521 aagctagatg tattagggat cttgatctct aatacaataa ctctcaaaac aatggctacc
    11581 actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc tcagatgatt
    11641 cagcgctttt attttatcac ttacgttatt aaataaccat cgttttatat agggaatttt
    11701 ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaggc ggattttata
    11761 attaacttag ccatttggaa ataaaaaaac gccacgtgaa ttccatgtta tattgaagtt
    11821 accacaactc tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt
    11881 tcacgcaagg gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag
    11941 gaaaactatt ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac
    12001 aacgaaatca aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc
    12061 tacgactatt atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac
    12121 cgcttgaact gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg
    12181 gacgacatga tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa
    12241 cacgaattgt tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac
    12301 cgtggcatca tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa
    12361 tcgtcccgtc atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc
    12421 ccggaggcgg ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga
    12481 aacaagacca agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag
    12541 gtcattctga agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg
    12601 agagaacggg ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc
    12661 tcggagttcg cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac
    12721 ccctatgctt cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc
    12781 atccccaagg gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt
    12841 tggatgaacg attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc
    12901 caggcattta ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc
    12961 ctcggcatgg cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc
    13021 ctcagctcat tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc
    13081 aaccgggtca ccatg
//
LOCUS       DADOZM010000080        13004 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.083, whole genome shotgun sequence.
ACCESSION   DADOZM010000080 DADOZM010000000
VERSION     DADOZM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.083"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            174..1310
                     /locus_tag="J8I47_001855"
     CDS             174..1310
                     /locus_tag="J8I47_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ6323288.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="J8I47_001856"
     CDS             1447..1791
                     /locus_tag="J8I47_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAZ6323289.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2062..3348
                     /gene="efmA"
                     /locus_tag="J8I47_001857"
     CDS             2062..3348
                     /gene="efmA"
                     /locus_tag="J8I47_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAZ6323290.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3486..4085)
                     /locus_tag="J8I47_001858"
     CDS             complement(3486..4085)
                     /locus_tag="J8I47_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAZ6323291.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4489..5610
                     /gene="mnmA"
                     /locus_tag="J8I47_001859"
     CDS             4489..5610
                     /gene="mnmA"
                     /locus_tag="J8I47_001859"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAZ6323292.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5856..7277
                     /gene="arcD"
                     /locus_tag="J8I47_001860"
     CDS             5856..7277
                     /gene="arcD"
                     /locus_tag="J8I47_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAZ6323293.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7291..8409
                     /locus_tag="J8I47_001861"
     CDS             7291..8409
                     /locus_tag="J8I47_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAZ6323294.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8469..9761)
                     /locus_tag="J8I47_001862"
     CDS             complement(8469..9761)
                     /locus_tag="J8I47_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ6323295.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9911..10471
                     /locus_tag="J8I47_001863"
     CDS             9911..10471
                     /locus_tag="J8I47_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323296.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10600..10977
                     /locus_tag="J8I47_001864"
     CDS             10600..10977
                     /locus_tag="J8I47_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAZ6323297.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10910..12982
                     /locus_tag="J8I47_001865"
     CDS             10910..12982
                     /locus_tag="J8I47_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ6323298.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4239 a         2327 c         2801 g         3637 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     2041 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     2101 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     2161 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     2221 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     2281 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     2341 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     2401 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     2461 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     2521 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     2581 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     2641 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     2701 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     2761 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     2821 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     2881 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     2941 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     3001 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     3061 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     3121 cgtgtcttag ggaacttcaa ctctttgatg aacctgacag gtccaattgg attattattt
     3181 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     3241 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     3301 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     3361 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     3421 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     3481 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     3541 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     3601 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     3661 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     3721 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     3781 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     3841 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     3901 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     3961 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     4021 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     4081 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     4141 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     4201 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     4261 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     4321 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     4381 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     4441 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     4501 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     4561 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     4621 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     4681 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     4741 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     4801 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     4861 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     4921 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     4981 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     5041 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     5101 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     5161 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     5221 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     5281 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     5341 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     5401 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     5461 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     5521 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     5581 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     5641 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     5701 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     5761 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     5821 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     5881 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     5941 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     6001 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     6061 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     6121 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     6181 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     6241 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     6301 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     6361 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     6421 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     6481 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     6541 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     6601 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     6661 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     6721 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     6781 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     6841 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     6901 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     6961 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     7021 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     7081 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     7141 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     7201 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     7261 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     7321 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     7381 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     7441 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     7501 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     7561 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     7621 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     7681 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     7741 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     7801 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     7861 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     7921 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     7981 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     8041 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
     8101 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
     8161 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
     8221 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
     8281 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
     8341 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
     8401 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
     8461 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
     8521 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
     8581 ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa tgcactggaa
     8641 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
     8701 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
     8761 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
     8821 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
     8881 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
     8941 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
     9001 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
     9061 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
     9121 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
     9181 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
     9241 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
     9301 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
     9361 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
     9421 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
     9481 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
     9541 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
     9601 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
     9661 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
     9721 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
     9781 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
     9841 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
     9901 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
     9961 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    10021 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    10081 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    10141 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    10201 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    10261 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    10321 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    10381 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    10441 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    10501 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    10561 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    10621 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    10681 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    10741 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    10801 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    10861 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    10921 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    10981 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    11041 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    11101 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    11161 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    11221 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    11281 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    11341 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    11401 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    11461 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    11521 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    11581 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    11641 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    11701 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    11761 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    11821 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    11881 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    11941 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    12001 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    12061 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    12121 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    12181 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    12241 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    12301 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    12361 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    12421 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    12481 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    12541 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    12601 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    12661 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    12721 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    12781 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    12841 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    12901 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    12961 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtga
//
LOCUS       DADOZM010000081        12967 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.084, whole genome shotgun sequence.
ACCESSION   DADOZM010000081 DADOZM010000000
VERSION     DADOZM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.084"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(260..979)
                     /locus_tag="J8I47_001866"
     CDS             complement(260..979)
                     /locus_tag="J8I47_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAZ6323299.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="J8I47_001867"
     CDS             complement(1028..2674)
                     /locus_tag="J8I47_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ6323300.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="J8I47_001868"
     CDS             2853..3443
                     /locus_tag="J8I47_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6323301.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="J8I47_001869"
     CDS             3535..5040
                     /locus_tag="J8I47_001869"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAZ6323302.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="J8I47_001870"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="J8I47_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAZ6323303.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="J8I47_001871"
     CDS             complement(6476..6994)
                     /locus_tag="J8I47_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323304.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="J8I47_001872"
     CDS             complement(7010..7336)
                     /locus_tag="J8I47_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAZ6323305.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="J8I47_001873"
     CDS             complement(7349..9328)
                     /locus_tag="J8I47_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ6323306.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="J8I47_001874"
     CDS             complement(9349..9663)
                     /locus_tag="J8I47_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAZ6323307.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="J8I47_001875"
     CDS             9831..10124
                     /locus_tag="J8I47_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAZ6323308.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="J8I47_001876"
     CDS             complement(10202..11314)
                     /locus_tag="J8I47_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ6323309.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11701..11880
                     /locus_tag="J8I47_001877"
     CDS             11701..11880
                     /locus_tag="J8I47_001877"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323310.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="J8I47_001878"
     CDS             12074..12244
                     /locus_tag="J8I47_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6323311.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12967)
                     /locus_tag="J8I47_001879"
     CDS             complement(12668..>12967)
                     /locus_tag="J8I47_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6323312.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4088 a         2609 c         2204 g         4066 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatc
//
LOCUS       DADOZM010000082        12851 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.085, whole genome shotgun sequence.
ACCESSION   DADOZM010000082 DADOZM010000000
VERSION     DADOZM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.085"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            185..619
                     /locus_tag="J8I47_001880"
     CDS             185..619
                     /locus_tag="J8I47_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6323313.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            720..1811
                     /locus_tag="J8I47_001881"
     CDS             720..1811
                     /locus_tag="J8I47_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAZ6323314.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1841..2518
                     /locus_tag="J8I47_001882"
     CDS             1841..2518
                     /locus_tag="J8I47_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAZ6323315.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2850..4145
                     /locus_tag="J8I47_001883"
     CDS             2850..4145
                     /locus_tag="J8I47_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323316.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4135..5595
                     /locus_tag="J8I47_001884"
     CDS             4135..5595
                     /locus_tag="J8I47_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6323317.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5731..7227
                     /gene="glpK"
                     /locus_tag="J8I47_001885"
     CDS             5731..7227
                     /gene="glpK"
                     /locus_tag="J8I47_001885"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAZ6323318.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7241..9064
                     /gene="glpO"
                     /locus_tag="J8I47_001886"
     CDS             7241..9064
                     /gene="glpO"
                     /locus_tag="J8I47_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAZ6323319.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9065..9781
                     /locus_tag="J8I47_001887"
     CDS             9065..9781
                     /locus_tag="J8I47_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAZ6323320.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10087..12015
                     /locus_tag="J8I47_001888"
     CDS             10087..12015
                     /locus_tag="J8I47_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAZ6323321.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            12141..12695
                     /locus_tag="J8I47_001889"
     CDS             12141..12695
                     /locus_tag="J8I47_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAZ6323322.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4302 a         2135 c         2912 g         3502 t
ORIGIN      
        1 cgactttttc acaacctctg attcacggtg ttccagaagg agttttgtcg aaaacaagcc
       61 gaaattcttt gaagatggct acttcttttt ttcaaatcta gacagaggag gcggacatta
      121 ttccgtgttt tttgacaaaa aataaaaatc atactatgat atgtacgcca atggagggga
      181 tgaaatggaa aagccaattg aaaaagcgtt agatgaatgt cttttcttta gcgtgaaaaa
      241 gctagatcgc atgttgaata aattagctga tgaagcgttt agaaggacag gtctggcacc
      301 aacttatggt tttattttat tgatattaaa agaaaaagat ggaataccac aaaaagatat
      361 tgcacaaatg ctctattccg ccccatcaac gattgctcgt tttgttgaaa aattagaata
      421 taaaggatac gtcaaaacag ttagcgaagg tcgcttgtca ttagtttatc ttacagaaca
      481 aggacgccaa tttgctaagg aagtcgatgg tagctgggaa gaacttcatc agtcatacaa
      541 agcagttttg ggaaatgaag caagtaaaga attagcccag acaatgaatg atgcaacaga
      601 taagttacaa aaaaaataaa attttttaag caaattagtt gcatgtacaa ctaatcgagt
      661 ggtttaatga tagatgattc tcgataggtt aatcagagcg aattatggga ggaatgacaa
      721 tgtcacaatt tgatcaatca attactttac cttcaggagt tcaattgaag aaccgattga
      781 tgctgtcgcc tatgacgaca caactcagct ttttcaatgg agtgatcacc gaagatgaga
      841 tcacttatta taaacaacgt tctaaaggtc ttggtgctgt tattactggt gctgcaaatg
      901 tccaagatat cggaaaaggc tggccaggtg aactcagcat cgctcatgat gaaatgattc
      961 ctcgattaag tgagctagcg aaagcaatcc aaggagaagg cgcaaaagcg attattcaga
     1021 ttttccacgg tggacgaatg acaagtcgag caactttatc tggagaacag cctgtatctg
     1081 caagtgcagt tgcagcaaaa cgtcctgatg cggaaacacc acgagcaatg agtgaagaag
     1141 aaatcgttga aacaatccaa gcatttggca aagccacacg tagagcgatt gaatcaggat
     1201 ttgatggaat cgagttgcat ggagcaaata cgtatttgat ccaacaattt ttctctccgc
     1261 attcgaatag acgagaggat caatggggcg gtagcttgga aaatcgttat cgttttattg
     1321 aagaattatt aacaacggtt ttctcagcgg ttgaacaata tgctaaaaaa ccattcatcg
     1381 tagggtatcg tttctcacca gaagaatatg aaacaccagg tatccgtttt gaagacacga
     1441 tctggcttct tgaacgatta cgtgagtcaa aactagatta tctgcatgtt tcattaaata
     1501 tgtatgatcg aatagctcgt tctgataaat acaacgataa atcgattctt gaagtggtcc
     1561 ataacacgat tcaaggaaag atccctttag ttggtgtcgg tgggatccgc aatcgcgaag
     1621 atgtagagca ggtattgaaa tatgcagaac ttgcagcaat tggacagcaa atgatcgtcg
     1681 atcctgactg ggcagagaaa ttattggaaa accgtgagga tgaatttgta acgaaacctt
     1741 ttgaagaagc atataaagag ctttatttgc caagcccatt atataatttc ttgaaagcac
     1801 gttatcaata acgcaatttt tatagaaaga ggatgggaaa atgaaagtat tgatcgtatt
     1861 aacaaatgta gaaaagtacg aaaagatcga gcgtcctaca ggtttgtggt taagtgaact
     1921 gactcatttt tatgatgtat tagtaaaaaa tgggatcgat gcagattttg tcagtcctaa
     1981 aggcggatat gtacctttag aaccgcaaag tgttttgaat atggatgaaa tcgattgggc
     2041 ctattatgaa gatgaagatt tcagaaatcg ggccttggga aataccttac gtcctgatga
     2101 tgtggatgca aatgattatg cagcggttta ttatgctggt ggccacggaa caatgtggga
     2161 ttttccagaa gcaatagatc ttgggaaagt cgcttcacaa atctatcgaa atgaagggct
     2221 ggtctcagct gtttgtcacg gtgttgtagg attgttgcca gttacagatg agtctggaaa
     2281 atctatttta gctggacgta cagtgactgg gttcagtaat gaagaagagg aagcaaatgg
     2341 tacaacgaat gaagtaccat tcttagcaga agatgctttg aaagaaaaag gagcaaacta
     2401 tcagtctgga gcagcttttt cagaagtggt tcaaattgat gggcggcttc ttacagggca
     2461 aaatcctcaa tctgctcatc aattaggcga agcagttgtt gaagcattaa agaattaaaa
     2521 ataaatgatg tgtctattct aactattaca ttacacacgc taaaactcgt cttttcgttc
     2581 ttttctgttt cctatttgga cagaggtacg taaaaggcga gtttttttct tttataagag
     2641 tacatttttt gaaacatctc ttcagaaatg atactcttca attaggtcca ctagtacatt
     2701 attgagaagc agacctagaa cacggtatga tggagagggt tgattctgct gcatcgctat
     2761 gtatcataag gttgaaagcg agttatgaat actgtgtttt ctgattgaga agttagcagt
     2821 agaacacctt ataagaaaga ggaatgcaga tgaacattgt aattgtcggc gggtcgtttg
     2881 cagggattca tgcggctatt tatcttcgcc aattgatgcc aacaagtgaa atatatttga
     2941 ttgaaaagca atcgaagtta ggatggattc ccagcgggtt caatttgata ttaaaaggaa
     3001 aggttaccag tgaaaaacaa ctttcttgga tcacgaaaga agaactgact gaccgctatc
     3061 gaatccatgt ttacacagaa aagacagtaa taggattgaa agaaaaaaaa gtcgttttag
     3121 caggaggaaa aaaattggat tttgatcgtt tgattctagc gactggttca aatcaaaagt
     3181 ttcgaaatat ttctgcagaa tcaacgctta ttcacccagt taaaaagata caaaacgtaa
     3241 cggctcttca gcaaaagatc atgaatgcaa agaaaattgc aattattggt gcaggacaag
     3301 caggaatcga aatagctgaa gggttggcat caagaaaaaa acaaatccat ctttacgaaa
     3361 gccgaaagtc aattcttttt cgttatctag atcctgaaat gacagaaccg ttagtaaagg
     3421 aaatgaaaaa tcaagggctt tcactttttt tagaagaaca agttgtgtct ttgaccgaaa
     3481 atgaaaccgc tattgtagag acggaaaaaa gaaaggagaa ctatgatctt gttttgctgg
     3541 ctaatcattc tcgccccgac aatcagatgt gggaagaaca attaacattg aatgatgatg
     3601 gaacgatctg ggtagatgac tacttgcaaa cgtctcaaaa agacgtttat gcgattggcg
     3661 atgcgatcca agtaactttt cgtccaacaa atgaaaaaat gtatgtttct ctagtaaata
     3721 acgctgtccg tacagcacga aatgtcagca agacaattag tggctcacag acaaaggatc
     3781 agggaaccta tcgtccagtc ggcaatcagt ggtttggcta tttcttagga agcgtaggtt
     3841 tgacagaaga agagagcatt ttttatcccc aaaaaattga aacaggatac gtagactaca
     3901 agcttacagc aataaatcaa agcaaagtta aactcaaatt tctatttgat tctgaaaaaa
     3961 agattcttgg agcacagctc caaagcaaaa aagagatgtt ccaccttctt gaccaactga
     4021 caattgcagt agaagaagga tggacgtttg aacagttaga agaacatgag ctatttttcc
     4081 agccagaata ccgtgtgcca gatatggtgt ggtcaaaggt ggcgaatact gcctatgaag
     4141 attgaagcat tattagaaag aaaagagcag ttacagatcc tgattttgcg aaaccttgtt
     4201 ctgcaagggg gaacagcctc tatcaatggt ttgagagagc atgtccaact gtcaaaagcc
     4261 tcgtttgatc aatatttgga agatattgaa ttgattggcc gaatgatgga aaaaaaagta
     4321 gaagtacagc gaaatgagta tcaggcgctg cttgtgttgg acgaagatgt aagtttggaa
     4381 aaaattctat tgtttttatt gcaggaaagc ttgaagttta agatgctcgt ctatttatta
     4441 gaacatcagc aagtatccat cgttcgatta gcgacagcgt ttaatatcag tgagtcttct
     4501 gtttttcgaa agatcaaaga attgaaccag ctcttagagg aatttggatt acaaatcaaa
     4561 aacggtcaat tatacggaga agagctacag atacgatatt tttattatga actattccaa
     4621 tatatcccgg aagatcagcg accgcttttt ttgcaaaata caccagaaaa aagaccgttc
     4681 attctaggat tggaccgtgt attagaaaca acgtttacgg catcagcaga agcacagatt
     4741 gcttgctggc tcgggattac gaaaaaaaga ctattgaatg aaaaaagtac gtacgcaact
     4801 ttgaaagaaa agaaattact ctatcagtcg gaccggcttt atcaagcaat cgatccaatc
     4861 atcgtgatgc atcttagtcg aacagcagca gaactgaatg tatacgaaac aatgatgttt
     4921 tacagctttt ttgtttcttt ttcgatagta gacgaagaaa tattttatca gtatgatttg
     4981 acacggagca aaaagttacc aaccgctgtt ttagatactt acattcggga aacgatgtta
     5041 tggcattatc gtcctcgtcg tttgaaaatc aaagaagaaa aggcagtagg ctaccaaatt
     5101 tcccagatca acaatgaatg gtttttcttt gttggaaaga tcgaagtcta tgagcgtgat
     5161 cggcttttgg aacagcagca gaaaatgctc gggcattcct taacacagtt actggcgaaa
     5221 ttacaggaaa cagcggtgca gcaattgccg aggaaaagag ctgaagatag tgagcttagt
     5281 tacctgatga tccagtatgc caatgttctt ttgatgatcg atttttatat tgcaaaacca
     5341 gtagtgattg gaatcgattt agaaagtctg ccgatttatc gtattgcttt tcaacaatat
     5401 ctgatcagag aactgagggg aatcggcggt attgagattg gaagttatga agaaggtaaa
     5461 gaatatgatt tggtgatcac attttgccag agaaacaaaa aacagtgtga atattatctg
     5521 tcagaatttg cgtcacctta cgacattatt cgattgaaaa gaagaataga gacgctgaaa
     5581 aaagaaaaga attgattaaa aaatagcttc ataataaaaa actgtcaaaa aaagaaaaaa
     5641 agaataaaat tttgacagtt tcttttttta aaacagtagt atgataagag tagttaaaga
     5701 aagcgtttgc atataatgga gggaatcaca atgacagaat caacgtatat catggcgatt
     5761 gaccaaggta caactagctc acgagcaatt atctttgaca agaaaggaag acacatcggc
     5821 agttctcaaa aagagttcac gcaatatttc ccgcaagaaa gttgggtgga acatgatgca
     5881 aatgaaatct ggaattctgt acagtcagtt attgccggag cgttcatcga atcagggatc
     5941 aaaccaaatc agattgctgg gattggcata acgaaccagc gtgaaacaac agtaatatgg
     6001 gaaaaagata caggacgtcc aatctatcat gcagttgttt ggcaatccag acagtcagct
     6061 ggaattgcag ataacttaaa aaatgaaggc tatcaagaat ttttccacga aaaaacaggg
     6121 ttagtgatcg atgcctattt ctcagcaacg aagatacgct ggattcttga tcatgtagaa
     6181 ggcgcgcaag aacgagctga aaaaggcgaa ttgatgtttg gaacgatcga ttcttggtta
     6241 gtatggaaac tgaccgatgg acaagcacat gttactgatt actcgaatgc cagtcggacg
     6301 atgttgttca atatccatca gctagattgg gatcaggaga ttttagattt actaaatatt
     6361 cctcgagtga tgttaccaaa agtcacttct aactcagaag tttatggtta tacgcaaggt
     6421 tatcattttt atggaagcga agcaccaatt tcagggatgg caggagacca gcaagccgca
     6481 ttattcggac aaatggcatt cgaaccaggc atggtcaaga atacttatgg tacaggttca
     6541 tttattgtca tgaataccgg tgaaaaaccg caattatcca aaaataattt attgacgaca
     6601 attggttacg gcataaatgg aaaggtttac tatgctttag aaggaagtat ttttgtggct
     6661 ggttcttcga ttcaatggct gagagatgga cttcaaatgt tgcaaaaagc tagcgattca
     6721 gaagacgcag cgaaacgctc tacaagcgaa gatgaggttt atgttgttcc tgcattcgtt
     6781 gggctaggtg ctccttattg ggatcaggca gcacgagggg ctatgtttgg cttgacacgt
     6841 ggtacgacaa aagaagatat cattaaagct actttacagt cgattgctta tcaagtgaga
     6901 gatatcatcg atacgatgca agatgacact ggaatcaaga ttcctgtatt gaaagtagat
     6961 ggcggtgcag caaacaatga atatctgatg caattccaaa cagatatctt gaatgtaccg
     7021 atccaacgag cagaaaactt ggaaacaacg gcgctagggg cagcattttt agcaggatta
     7081 gctgtcggtt attggaaaga tacagacgag atccgcgaat tttatgaagc tggcaagcta
     7141 tttgaagttc aaatggagga agagcgccga gaaaaacttt acaacggttg gaaaaaagca
     7201 gtaaaagcta cccaagcatt tgaataagga ggaaatccat atgttttcaa acaagacaag
     7261 acaagatagc attcaaaaaa tgcagcaaga agaattggat ctgttgatca tcggtggcgg
     7321 aatcactggt gccggtgtgg cagtccagtc agcagcatca ggaatcaaaa caggattgat
     7381 cgaaatgcag gattttgcag aagggacttc ctctcgctcg accaaacttg ttcatggcgg
     7441 tattcgttat ctgaagacat ttgatgtgga agtagtagct gacacagttg gtgaacgtgc
     7501 agtcgtacaa agtattgccc cacacatccc aaaaccagat ccaatgcttt tgccaatcta
     7561 tgagggcgaa ggagcaacaa ccttcaatat gttctcggtc aaagtagcaa tggaccttta
     7621 tgacaaactg gcaaatgtga caggaaccaa atatgagaat tatacgctga caccagaaga
     7681 agtattggaa agagaaccgt ttttgaaaaa agaagggcta aaaggcgcgg gtgtctatct
     7741 agatttccgc aacaatgatg cccgtttagt gattgataat atcaaaaagg ctgccgaaga
     7801 tggggcttat ctagtaagta aaatgaaagc ggttggcttt ttatatgatg gcgatcaaat
     7861 cgttggcgtc aaagctcgtg acctgctgac agatgaagtg atcgaaatca aagcaaaatt
     7921 agtgatcaat acgagtggtc cttgggtaga taaagtacga aacttgaatt ttacgcgtcc
     7981 agtctctcct aaaatgcgtc caactaaagg gatccattta gttgtagatg cgaaaaaact
     8041 gcctgtaccg caacccacat acttcgatac aggaaagcaa gatgggcgga tggtttttgc
     8101 tattccaaga gaaaacaaga cttactttgg tacgacagat acggattacc aaggagattt
     8161 tacggatccg aaagtcacac aagaagatgt agattatcta ttggatgtga tcaatcatcg
     8221 ctatccagaa gcaaatatca cattgaatga tatcgaagca agctgggcag gacttcgtcc
     8281 actgttgatt ggtaattctg gttctgatta caatggtgga gataatggat cgatttcaga
     8341 caagagcttc aataaagttg tcgatacagt aagtgaatat aaggaaaaca aagtttctcg
     8401 tgctgaagta gaagacgtgt tgaaccattt ggaaaacagc cgtgatgaaa aagcaccttc
     8461 tacgatttcc agaggtagtt ctttagaaag agaaccagat ggtttgctga ctttatctgg
     8521 tgggaaaatc accgattacc gtaagatggc agaaggagct ttgcgattga tacgccaatt
     8581 attaaaagaa gaatacggaa tagagacgaa agaaatcgac tctaaaaaat atcagatttc
     8641 aggtggaaac ttcgatccaa ctaaattaga agaaacagtg acagaattag caaaagaagg
     8701 agtagcagcc ggtttagagg aagaagatgc tacttatatc gctgattttt acgggactaa
     8761 tgcccgacgt atctttgaat tagcaaaaga aatggcacct tatcctggtt tgagtctcgc
     8821 tgagtcagct cggttacgtt atggtttaga agaagaaatg gttttggccc caggcgatta
     8881 tctcattcgt cgtacaaatc atctgttgtt tgaacgagat cagcttgatg agatcaagca
     8941 acctgtgatc gatgcaattg ctggatattt tgaatggaca gaagaggaga aggcgcaaca
     9001 gactaaacgt ttagaagcat tgattgcaga atcagatctg cgggaactaa agggggagaa
     9061 ataaatgaac agtgatatga ctcaaatcat gggagagttt attgggacac tgatcctagt
     9121 tttgcttgga gatggtgtgt gtgcggctgt gaatttaaac aaaagtaaag cacaggcttc
     9181 tggctggatt gtcatcgcat ttggttgggg actcgctgta acaatggctg tctatatttc
     9241 tggattcatg ggcccagctc atttgaatcc tgcagtctct ctagctatgg cgatgacagg
     9301 ggcaattagt tggaatcttg tcgttccttt catcattgcg caagttttag gagcttttgc
     9361 aggggccatt cttgtttggc tttcttattt gccacattgg aatgcaacga aagatgaaag
     9421 cgcgatttta ggaacgtttg caacaggtcc tgctatacgg aattatccag caaatgtgat
     9481 cacagaatta attggtacat ttgtattagt tcttgggttg ctagcttttg gacaaaacga
     9541 atttgcacct ggaacgaacg tattcgctgt aggtggattg atcttagcca tcggtctttc
     9601 attaggaggg ccaacaggat atgcaatcaa ccctgctcgg gactttggtc ctcgattagc
     9661 acatgcggta ttgccaatag ctaataaagg aacttcagat tgggcatatt catgggtacc
     9721 aatcgcaggt ccaatgatcg gagcaattat cgcggtaggt gtctattcat taatggtgta
     9781 aatctataaa acatactaaa gtttgacaac caaaatgtgt cttgctatag ttaagtggaa
     9841 aataaataag acctagaaga atggggtatg ttttgtgagt gtagctggta tctaatcagt
     9901 aagtcgaata gccaataaac aaagtttatt tggtgtgctt atgaaacggt acctgtcagg
     9961 aaaacaatat ctttgtattc taataaggat aggacatgac ttttttaagc tgtgttctat
    10021 ccttttattt atctctaggc ccttatttat tttgggacag gtatctcttc aagaaaagga
    10081 gagaatatgg acaacgaaat gattagtcaa ttacaatttg atcggatcaa aaaggaaatc
    10141 caagcaagag caattggaaa ctatagcaaa aaacgaatca gcgatatgac ggtctcgaca
    10201 aacctacaaa ctgtcttgga tcgtcaagaa gaaacaagag aagcacgctt gatcttagaa
    10261 agcagtcagc atgttccatt tatggggttg ccgaggatcg atgcactgac agaacaagtc
    10321 aaaaaagggt tgattctgca gccaaccgat ttgatcgaat atgcggattt tcttagaagc
    10381 agtcggatga tcacaaagtt ttttgataag aatcaatacc agacaccctt gttgtttgct
    10441 tatagcaaac atcttcctga tttgataaat gtagaagagt tgattgacca gaaaataaaa
    10501 aataataaag tcagtgacga cgcgtcacgt aatttgcgta aagtacgcaa acaattacaa
    10561 ctaatcgaaa aagaaatcca gtcaaaatta ctgaaattct tgcgtcatcc gaaaaacaaa
    10621 gaaatgatcc aagaagcaat gattgttcaa aaaggcgaat actatacgat tcctatcaaa
    10681 gccagctata aaaataaaat agatggaacg attatcgatg aatcaaataa aagaacgacg
    10741 gtttttattg aaccaactgt gatttctaaa ttgaatgaac actatcagtt gttaaaagct
    10801 gaagaaatca gtgaagaata tcaagtcctg gcggcattga ctggtgctat cgcagaaaac
    10861 gaagaagtga tcgatttatt gatcgaaacg atcacggtgt tagatatcat ctttgctcgg
    10921 gcaaaattta gccgagaaat caatggaagc acacctagaa tcaataaatc ggaacatatc
    10981 attatcaagc aaggcagaca tccattttta cctgagcatg cggtaccatt ggatgttgaa
    11041 atcggtaaag actatcgagg cttgattatc accggagcaa atgccggagg caaaacagtc
    11101 gtgttgaaga cagtcggttt attgactttg atggcgatgt tcggtatgca agttccggca
    11161 aaagaaggga cagagcttgc tgtctttgat gagattttcg tcgatgtcgg tgaccaccaa
    11221 aatttagaaa atgcgttgag tacattttct ggtcatatgc aaaacatcgc tgctattttg
    11281 aaaaagataa aaaggaatac gttggtactt ttagatgaga ttggcagtgg aacagagcca
    11341 aatgaagggg ctgcactagc gatcgcaatc atggaatcta tgtatgagca aggggcattg
    11401 atcattgcca cgacccatta tggagaaatc aagaaattcg ccggagatca tgaagatttt
    11461 gtgccggctg ctatggcttt cgatcgggaa gcacttcgtc ctaaatatca gttgcgggtt
    11521 ggagaaacag gagaaagcca agctttgtgg attgctcata aaatggatat gtcgatgaaa
    11581 ttgatccatc aagcagagcg ttacttggcg gaaaaagcct atcggacaca gaaaaaagaa
    11641 tttcttccta agacagcagt tacaaatagg aaggagaata aaaaagaacg ccagctcttt
    11701 gcaaaaggtg atcggatatt tgtcaatgaa taccaaaaag aagcgttagt ttatgaagat
    11761 ataggtgaag atacgattga tgtatattta gacaaaaaaa taatccacgt gcctcgtcag
    11821 cgtgttcgtc tggtgcgttc agcagaagat ctgtacccga caggttacga tttagacagt
    11881 ctgtttatcg attataaaac acgtaaacgg cagcgtgacc tagagcgagg atcaaaaaaa
    11941 gctcataaag tactagtaaa agaaatgcgc aaacgacagg aagaaagaag agtaaacgat
    12001 gaaaacagca agtgattatc tcaaattagc tcataatatc agtggctgta aagaaaaata
    12061 aaaacagtga aatcattggt tttattcccc gtgtaagcta aaataaaaag aaaagagtgc
    12121 tgaggaggta gggcgataaa atgaaattaa gcacagactg gcgtaaagaa atacaaacga
    12181 ttccgaatct tttatcgata ttccgtattt ttttgctgcc gatttatctt tattttgtgc
    12241 tgagacagtc attttatata gcaggagcgg tcattgttgt ctcaggtttg tctgattatc
    12301 tagacggggt cattgctcgg agatataatc aggtaacaga cttagggaag gtgcttgatc
    12361 cgtttgcaga taaattgaca caactttttc tgattttgtc tatggcatgg tatcgaccgt
    12421 ggctttggct gttgtttggt ctattcttga taaaagaagg ctttatgttt gtagctggat
    12481 taatcgggct atctaaaaat atcaaattaa gtggagcgaa gtggtatggc aaagtagcga
    12541 ctgctgtcat ctatgttggc atgatccttt tgctgctttt cccagaatta ccaacgctgt
    12601 gggtgagagt catttttgcc gtcatcactt acggtctttt acaatctttt gtcttgtatg
    12661 ctgtcgagta ccgaaagatg tttcaaagaa aataaaaaag gggtgtgacc tatgtcgcat
    12721 tccttttttt gataaataga ctcccagtag ctacttctcg ataataagcc aaccaaacca
    12781 aaaacaggag aagttgtttt tgaaattatt tcttgtgatt cggagttaaa tggtaggctc
    12841 acaacctctt g
//
LOCUS       DADOZM010000083        12773 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.086, whole genome shotgun sequence.
ACCESSION   DADOZM010000083 DADOZM010000000
VERSION     DADOZM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.086"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(367..729)
                     /locus_tag="J8I47_001890"
     CDS             complement(367..729)
                     /locus_tag="J8I47_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323323.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(803..1183)
                     /locus_tag="J8I47_001891"
     CDS             complement(803..1183)
                     /locus_tag="J8I47_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAZ6323324.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1265..2440
                     /locus_tag="J8I47_001892"
     CDS             1265..2440
                     /locus_tag="J8I47_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ6323325.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2517..3101)
                     /locus_tag="J8I47_001893"
     CDS             complement(2517..3101)
                     /locus_tag="J8I47_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ6323326.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3302..4300
                     /locus_tag="J8I47_001894"
     CDS             3302..4300
                     /locus_tag="J8I47_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323327.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4412..4921
                     /locus_tag="J8I47_001895"
     CDS             4412..4921
                     /locus_tag="J8I47_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAZ6323328.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4951..5610)
                     /locus_tag="J8I47_001896"
     CDS             complement(4951..5610)
                     /locus_tag="J8I47_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAZ6323329.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5612..6376)
                     /locus_tag="J8I47_001897"
     CDS             complement(5612..6376)
                     /locus_tag="J8I47_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAZ6323330.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6384..7061)
                     /locus_tag="J8I47_001898"
     CDS             complement(6384..7061)
                     /locus_tag="J8I47_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAZ6323331.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7098..8483)
                     /locus_tag="J8I47_001899"
     CDS             complement(7098..8483)
                     /locus_tag="J8I47_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAZ6323332.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8693..8777)
                     /gene="ffs"
                     /locus_tag="J8I47_001900"
     ncRNA           complement(8693..8777)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J8I47_001900"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="J8I47_001901"
     CDS             complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="J8I47_001901"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAZ6323333.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9422..10279
                     /locus_tag="J8I47_001902"
     CDS             9422..10279
                     /locus_tag="J8I47_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAZ6323334.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10360..10695)
                     /locus_tag="J8I47_001903"
     CDS             complement(10360..10695)
                     /locus_tag="J8I47_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323335.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10762..12132)
                     /locus_tag="J8I47_001904"
     CDS             complement(10762..12132)
                     /locus_tag="J8I47_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAZ6323336.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12210..12746)
                     /locus_tag="J8I47_001905"
     CDS             complement(12210..12746)
                     /locus_tag="J8I47_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323337.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3792 a         2592 c         2132 g         4257 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      541 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      601 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      661 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      721 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      781 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
      841 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
      901 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
      961 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1021 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1081 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1141 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1201 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1261 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1321 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1381 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1441 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1501 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1561 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1621 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1681 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1741 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1801 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     1861 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     1921 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     1981 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2041 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2101 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2161 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2221 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2281 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2341 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2401 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2461 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2521 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2581 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2641 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2701 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2761 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     2821 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     2881 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     2941 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3001 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3061 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3121 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3181 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3241 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3301 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3361 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3421 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3481 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3541 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3601 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3661 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3721 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3781 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     3841 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     3901 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     3961 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4021 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4081 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4141 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4201 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4261 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4321 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4381 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4441 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4501 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4561 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4621 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4681 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4741 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4801 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     4861 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     4921 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     4981 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5041 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5101 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5161 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5221 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5281 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5341 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5401 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5461 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5521 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5581 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5641 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5701 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5761 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     5821 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     5881 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     5941 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6001 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6061 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6121 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6181 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6241 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6301 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6361 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6421 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6481 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6541 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6601 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6661 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6721 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6781 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     6841 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     6901 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     6961 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7021 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7081 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7141 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7201 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7261 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7321 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7381 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7441 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7501 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7561 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7621 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7681 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7741 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7801 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     7861 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     7921 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     7981 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8041 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8101 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8161 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8221 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8281 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8341 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8401 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8461 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8521 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8581 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8641 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8701 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8761 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     8821 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     8881 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     8941 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9001 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9061 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9121 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9181 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9241 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9301 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9361 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9421 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9481 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9541 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9601 aaaaaaacac ccatggtatc gctatctttt taaagatgct tcttgtgttc aagaaaaaag
     9661 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9721 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9781 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
     9841 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
     9901 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
     9961 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10021 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10081 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10141 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10201 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10261 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10321 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10381 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10441 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10501 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10561 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10621 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10681 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10741 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10801 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    10861 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    10921 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    10981 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11041 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11101 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11161 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11221 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11281 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11341 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11401 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11461 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11521 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11581 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11641 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11701 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11761 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    11821 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    11881 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    11941 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12001 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12061 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12121 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12181 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12241 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12301 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12361 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12421 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12481 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12541 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12601 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12661 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12721 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acc
//
LOCUS       DADOZM010000084        12735 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.087, whole genome shotgun sequence.
ACCESSION   DADOZM010000084 DADOZM010000000
VERSION     DADOZM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.087"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="J8I47_001906"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="J8I47_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAZ6323338.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="J8I47_001907"
     CDS             complement(323..892)
                     /locus_tag="J8I47_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAZ6323339.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="J8I47_001908"
     CDS             1078..1980
                     /locus_tag="J8I47_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6323340.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="J8I47_001909"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="J8I47_001909"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAZ6323341.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="J8I47_001910"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="J8I47_001910"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAZ6323342.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="J8I47_001911"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="J8I47_001911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAZ6323343.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="J8I47_001912"
     CDS             8548..8733
                     /locus_tag="J8I47_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAZ6323344.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="J8I47_001913"
     CDS             complement(8795..9991)
                     /locus_tag="J8I47_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ6323345.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="J8I47_001914"
     CDS             complement(10161..11597)
                     /locus_tag="J8I47_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAZ6323346.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="J8I47_001915"
     CDS             11890..12699
                     /locus_tag="J8I47_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6323347.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4009 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctagtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca cgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgaca
//
LOCUS       DADOZM010000085        12670 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.088, whole genome shotgun sequence.
ACCESSION   DADOZM010000085 DADOZM010000000
VERSION     DADOZM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.088"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            6..305
                     /locus_tag="J8I47_001916"
     CDS             6..305
                     /locus_tag="J8I47_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323348.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="J8I47_001917"
     CDS             378..1739
                     /locus_tag="J8I47_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAZ6323349.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="J8I47_001918"
     CDS             1761..2021
                     /locus_tag="J8I47_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323350.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="J8I47_001919"
     CDS             2035..2787
                     /locus_tag="J8I47_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAZ6323351.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="J8I47_001920"
     CDS             complement(2881..3486)
                     /locus_tag="J8I47_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6323352.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="J8I47_001921"
     CDS             complement(3539..4513)
                     /locus_tag="J8I47_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAZ6323353.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="J8I47_001922"
     CDS             complement(4891..5670)
                     /locus_tag="J8I47_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323354.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8I47_001923"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8I47_001923"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAZ6323355.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="J8I47_001924"
     CDS             7161..8558
                     /locus_tag="J8I47_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6323356.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="J8I47_001925"
     CDS             8639..9079
                     /locus_tag="J8I47_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ6323357.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="J8I47_001926"
     CDS             9227..9553
                     /locus_tag="J8I47_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ6323358.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="J8I47_001927"
     CDS             9554..10303
                     /locus_tag="J8I47_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323359.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="J8I47_001928"
     CDS             complement(10383..10838)
                     /locus_tag="J8I47_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ6323360.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="J8I47_001929"
     CDS             complement(11265..11540)
                     /locus_tag="J8I47_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323361.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="J8I47_001930"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="J8I47_001930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAZ6323362.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DADOZM010000086        12417 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.089, whole genome shotgun sequence.
ACCESSION   DADOZM010000086 DADOZM010000000
VERSION     DADOZM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.089"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(122..808)
                     /locus_tag="J8I47_001931"
                     /pseudo
     CDS             complement(122..808)
                     /locus_tag="J8I47_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(944..1138)
                     /locus_tag="J8I47_001932"
     CDS             complement(944..1138)
                     /locus_tag="J8I47_001932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323363.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1188..1628)
                     /locus_tag="J8I47_001933"
     CDS             complement(1188..1628)
                     /locus_tag="J8I47_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAZ6323364.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1632..2477)
                     /locus_tag="J8I47_001934"
     CDS             complement(1632..2477)
                     /locus_tag="J8I47_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAZ6323365.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2626..4044
                     /gene="pepV"
                     /locus_tag="J8I47_001935"
     CDS             2626..4044
                     /gene="pepV"
                     /locus_tag="J8I47_001935"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAZ6323366.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4101..5048)
                     /locus_tag="J8I47_001936"
     CDS             complement(4101..5048)
                     /locus_tag="J8I47_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ6323367.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5271..5621)
                     /locus_tag="J8I47_001937"
     CDS             complement(5271..5621)
                     /locus_tag="J8I47_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAZ6323368.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5821..6900
                     /gene="pepA"
                     /locus_tag="J8I47_001938"
     CDS             5821..6900
                     /gene="pepA"
                     /locus_tag="J8I47_001938"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAZ6323369.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7044..7364
                     /locus_tag="J8I47_001939"
     CDS             7044..7364
                     /locus_tag="J8I47_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ6323370.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7386..7850
                     /locus_tag="J8I47_001940"
     CDS             7386..7850
                     /locus_tag="J8I47_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ6323371.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8055..8660
                     /locus_tag="J8I47_001941"
     CDS             8055..8660
                     /locus_tag="J8I47_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAZ6323372.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8699..9988)
                     /locus_tag="J8I47_001942"
     CDS             complement(8699..9988)
                     /locus_tag="J8I47_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAZ6323373.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10416..10895
                     /gene="rlmH"
                     /locus_tag="J8I47_001943"
     CDS             10416..10895
                     /gene="rlmH"
                     /locus_tag="J8I47_001943"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAZ6323374.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            11098..11648
                     /locus_tag="J8I47_001944"
                     /pseudo
     CDS             11098..11648
                     /locus_tag="J8I47_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            11719..>12417
                     /locus_tag="J8I47_001945"
     CDS             11719..>12417
                     /locus_tag="J8I47_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAZ6323375.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGI"
BASE COUNT         3992 a         2261 c         2412 g         3752 t
ORIGIN      
        1 aataaacggt gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg
       61 aggaactatt ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct
      121 cttatttgat gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt
      181 attcttgctc ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa
      241 agttatgatc aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt
      301 ctaattctga aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt
      361 ctagccaatc ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag
      421 tatccaaatc aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta
      481 gtattttttc tagtttagct tgatcttact tggtcatttt cgcactgcat tcttgcagga
      541 tcggtttttc caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt
      601 ttgaaacata agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga
      661 tcagtgcttc tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg
      721 agattttact tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag
      781 cttgctgttg caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat
      841 tataacataa atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc
      901 tgataggcat gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct
      961 cttggatcgg tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt
     1021 ctttgaaaat gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga
     1081 tgtaaccgtg cattacttta acgacataca tggggatcac ctgctttttt cttttcatta
     1141 tacgaactac tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca
     1201 tcatccaaag aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc
     1261 ttcactaatc gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt
     1321 aattttttgt cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac
     1381 ttttggatag ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg
     1441 caatttactt ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac
     1501 cctagatgta ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga
     1561 tgaataatcc ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat
     1621 ttctccatga ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc
     1681 ggaagtgctt cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct
     1741 ccaatgaaac tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg
     1801 gctaaaaatc ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca
     1861 gcagaaccta aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt
     1921 ttcaaaacga gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt
     1981 tcttgtatcg gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt
     2041 tgttcgggat agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca
     2101 atcaccagcc attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc
     2161 ttagaagaag tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa
     2221 agaaactggt cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga
     2281 gcactccgat ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca
     2341 gctaaagcac tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata
     2401 cgtccaaatg ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt
     2461 tgttcgttta tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag
     2521 agaaaaacag ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg
     2581 tggtatcatg aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg
     2641 caaaaagaag tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt
     2701 gtcaacagcg agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct
     2761 cgcgacgcat tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat
     2821 gtcgataact atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt
     2881 ggacacatgg atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta
     2941 atcaaggatg gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca
     3001 gcttactatg cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc
     3061 ttcgtagtcg gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat
     3121 gaagaagaac cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa
     3181 aaaggaaatg tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg
     3241 aaatcttttg tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt
     3301 caagtaccaa gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa
     3361 gcaaatccag taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt
     3421 ggtaaaggcg cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca
     3481 ttgttcttag ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct
     3541 gcttatgtcc atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa
     3601 atgggcaaat tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa
     3661 gcgaacttca tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa
     3721 agtgacatcg aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta
     3781 atggaaccac attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat
     3841 gaagaccaca ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt
     3901 ttgttgaaac gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat
     3961 caagcaaacg aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat
     4021 gccatctatc gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt
     4081 ttgcagtctt gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga
     4141 ttcaattcat attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact
     4201 aatgaaagaa gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta
     4261 ataaagcttg tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa
     4321 aagaagaaga tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta
     4381 ataatcatca tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct
     4441 tcaggacgat cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca
     4501 tggatcgtca attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca
     4561 aagatacgat tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt
     4621 ctagtaattg tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca
     4681 tcaaagtagg ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt
     4741 aatttctcga gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt
     4801 aaataatagt cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga
     4861 cgaggttctt tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc
     4921 gaattattat catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg
     4981 actgttgaaa ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt
     5041 tgttccattt ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat
     5101 aatcaaagga aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg
     5161 aatatatgtt tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac
     5221 ctctcttttg aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt
     5281 gcaccttttt ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca
     5341 taataaacag tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga
     5401 acggcgaatt tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct
     5461 tcttttagaa aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg
     5521 cgatgatacc aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct
     5581 aacccgcctt taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt
     5641 ctcacttgtt actccttgtg cttattttac cacactctca tattttcttt gaaataaata
     5701 accgctacca gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt
     5761 ttatccgcag atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat
     5821 atggaagaaa aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt
     5881 tttgaacaag atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta
     5941 cagttagatg gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc
     6001 cgtgtaatgg tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa
     6061 aacgggcttt ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag
     6121 cgttttacac ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc
     6181 ccacatttat tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc
     6241 gatgctgggt ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca
     6301 atcgttcctt acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca
     6361 tgggacaacc gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa
     6421 acattaggtc atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt
     6481 tctaaagctt ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca
     6541 gcagatgata cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg
     6601 cgtatccaag atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc
     6661 gctgaaacaa acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt
     6721 gctgctcata cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat
     6781 atccatacgc atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg
     6841 attcaaacac tgaaaggttt ggataaatca acagtaaata cgatcgtata cggaaaataa
     6901 tgcgatagtg aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa
     6961 aaagaggatc aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat
     7021 aaacagaaag tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct
     7081 acgtagaaac tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt
     7141 ttatcaaacc gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag
     7201 tagaccgtga caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt
     7261 ttgtcgtgat ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa
     7321 aacaggaaat cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg
     7381 tcaagatgaa tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac
     7441 ttgctgcttt caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag
     7501 caaacgtaat cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc
     7561 tagtcgatca gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga
     7621 aatctgttga ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca
     7681 tggcagtcaa attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc
     7741 taaatgcagt cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac
     7801 cttgtgatgt cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa
     7861 cttgtacgac ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg
     7921 tcacagtccc ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca
     7981 tgaaaaaaat ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata
     8041 atggaggaaa gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg
     8101 ttattatagc cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc
     8161 ggatcaaaga tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc
     8221 taacaatcga agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc
     8281 gattgatcaa agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg
     8341 ttgtcgggta cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg
     8401 agacagaagt agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag
     8461 ctggacaaaa agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct
     8521 ggccaggaac tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc
     8581 atttgccaaa tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg
     8641 gagaagcatt tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt
     8701 aattttcact attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag
     8761 ttgttttttc tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct
     8821 tatacaaagc agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa
     8881 tgacgtcgta ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca
     8941 cgatgacacc gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag
     9001 aaacggcaga taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt
     9061 tttccagttg attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt
     9121 ctacgcttac attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga
     9181 taacttgtcc ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat
     9241 ctgtttggat cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc
     9301 ggttcaagga agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg
     9361 aaccaatcgc aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa
     9421 ctgtttcgac tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc
     9481 caactaattc agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc
     9541 catctactac gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca
     9601 cgccgcttcc ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt
     9661 gctgcccgaa tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag
     9721 aaacgacagc gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt
     9781 tgacattttc cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac
     9841 tattgttgcc agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga
     9901 tccctccaac aagccccaga cctaacttac gccagaggct attattttta ctttttttca
     9961 tctttggtgt cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta
    10021 ctgttagtat agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga
    10081 aaaactttag gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga
    10141 tactgtgtaa aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc
    10201 tgtgtataaa gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat
    10261 aaggtaaaat aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg
    10321 gaaatctttt taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata
    10381 aacaaagtga gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag
    10441 gaaaattaaa agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg
    10501 cctatgcaaa aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg
    10561 aagcgcaaat gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag
    10621 aacaagaata cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg
    10681 ctaaacaaat cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg
    10741 gctctttagg actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa
    10801 aaatgacgta tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct
    10861 tccggatcaa tgcgggggct ccataccaca aataatgata ttttttaaga gaaaaaatta
    10921 tccctatttt aaataatagc aataggtagt ggtagggaga aatttataaa taactttttt
    10981 atttgctttg gtagccaagt aagttgcaaa agaaaaatag atactgttaa aaagataatg
    11041 tagacataga aacaaatacg taaaatttta gttagaataa tggcaaggag aaaaaacatg
    11101 atagatgaaa aaaagtgttt aaaaaatcta ttagaggata tggatgatat tgaagaaaac
    11161 tatctaaatg gactgggaaa acaaatatat ttcagatact taagctatct aaaaatgaaa
    11221 taagacatag taatttttta gcttttttgt ttgctccaaa tgaaacccac aatttatctg
    11281 atgagttttt taaaatgttt ttgaaaaggt atatagattc taatgatgat accaaagctg
    11341 ctatcaatta ttttgatgct ttacttaata gctatgaaga tttaattgtt tacagagaaa
    11401 ataagaatat tgatatttta ttggtatcgg aaaaaaataa aatagtggtg tgcatcgaaa
    11461 ataaaattct gtcttcggag tctggagggc aacttaataa ataccaaata tatatagaac
    11521 gcaattattc taactataaa aaaatttttg tttttttaac tccagatgga aatgaaccca
    11581 caaatcctag ttggggcata attacttaca agaatatcgt tgaagagagt gtaaaatatt
    11641 ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac
    11701 agaaaagagg acttccatat gaacgatttt actacagaaa ttatggaaat actaatcaat
    11761 aaaggtgatt tggatgattt gtttcgtcgt cacctagaat tggctattaa cgctttatta
    11821 caagctgaat taacggtatt tcttgactat gaaaagtatg atagagctgg atttaattca
    11881 ggtaattccc gtaatgggaa ttattcgcgc tcttttaaaa cagagtacgg tgaattgaat
    11941 ttagtaatac ctagagatag gaatggagac ttttctcaac aaactttacc tgcttataaa
    12001 agaaccaatg actctttaga aaccactatt attcagctag ttcaaaaagg aattactatg
    12061 tccgaaatct ctgatctaat cgaaaaaatg tatggtcatt attacaaacc acaaactatt
    12121 tcaaatatga gtaaaattgt gtctgaagat gtttcggcat ttaaagaaag aactttagaa
    12181 gctaaatact cagtcatttt tatggatgct actcatattc ccttaaaaag acaaaccgta
    12241 tcaaaagaag ctgtctatat agtaattggt atcaggttgg atggaactaa agaggttcta
    12301 ggatttacta tagctccaac tgaatctgct tatgtttgga aagagattct tcaagatcta
    12361 aaagaccgtg gtttagaaga ggttttgtta gtcgtaacag atggtttaag tggtatt
//
LOCUS       DADOZM010000087        12204 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.090, whole genome shotgun sequence.
ACCESSION   DADOZM010000087 DADOZM010000000
VERSION     DADOZM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.090"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(147..488)
                     /locus_tag="J8I47_001946"
     CDS             complement(147..488)
                     /locus_tag="J8I47_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAZ6323376.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="J8I47_001947"
     CDS             complement(556..2727)
                     /locus_tag="J8I47_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAZ6323377.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="J8I47_001948"
     CDS             2938..3795
                     /locus_tag="J8I47_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ6323378.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="J8I47_001949"
     CDS             complement(3866..4723)
                     /locus_tag="J8I47_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6323379.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="J8I47_001950"
     CDS             complement(4708..7410)
                     /locus_tag="J8I47_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ6323380.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="J8I47_001951"
     CDS             complement(7423..8712)
                     /locus_tag="J8I47_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAZ6323381.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="J8I47_001952"
     CDS             8862..9908
                     /locus_tag="J8I47_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323382.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="J8I47_001953"
     CDS             9910..12063
                     /locus_tag="J8I47_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ6323383.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3362 a         2578 c         2230 g         4034 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagca ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcaattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca gggcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagca tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataaacc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgactcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacagaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat
    12121 agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc
    12181 gtttattgag aggttgtgaa atca
//
LOCUS       DADOZM010000088        12196 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.091, whole genome shotgun sequence.
ACCESSION   DADOZM010000088 DADOZM010000000
VERSION     DADOZM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.091"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..490
                     /locus_tag="J8I47_001954"
     CDS             <1..490
                     /locus_tag="J8I47_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6323384.1"
                     /translation="LLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGY
                     EVYQVFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNG
                     PIECLNNHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKI
                     AV"
     gene            complement(731..2347)
                     /locus_tag="J8I47_001955"
     CDS             complement(731..2347)
                     /locus_tag="J8I47_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ6323385.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2465..2683
                     /locus_tag="J8I47_001956"
     CDS             2465..2683
                     /locus_tag="J8I47_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323386.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            2878..3339
                     /locus_tag="J8I47_001957"
     CDS             2878..3339
                     /locus_tag="J8I47_001957"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6323387.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3532..3771)
                     /locus_tag="J8I47_001958"
     CDS             complement(3532..3771)
                     /locus_tag="J8I47_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323388.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3795..5318)
                     /locus_tag="J8I47_001959"
     CDS             complement(3795..5318)
                     /locus_tag="J8I47_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAZ6323389.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5336..5458)
                     /locus_tag="J8I47_001960"
     CDS             complement(5336..5458)
                     /locus_tag="J8I47_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAZ6323390.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5488..6471)
                     /locus_tag="J8I47_001961"
     CDS             complement(5488..6471)
                     /locus_tag="J8I47_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ6323391.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6495..6644)
                     /gene="rpmG"
                     /locus_tag="J8I47_001962"
     CDS             complement(6495..6644)
                     /gene="rpmG"
                     /locus_tag="J8I47_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ6323392.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6665..7042)
                     /locus_tag="J8I47_001963"
     CDS             complement(6665..7042)
                     /locus_tag="J8I47_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323393.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7074..7253)
                     /gene="rpmF"
                     /locus_tag="J8I47_001964"
     CDS             complement(7074..7253)
                     /gene="rpmF"
                     /locus_tag="J8I47_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAZ6323394.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7268..7537)
                     /gene="rpsN"
                     /locus_tag="J8I47_001965"
     CDS             complement(7268..7537)
                     /gene="rpsN"
                     /locus_tag="J8I47_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAZ6323395.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8060..9832)
                     /locus_tag="J8I47_001966"
     CDS             complement(8060..9832)
                     /locus_tag="J8I47_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323396.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9786..11540)
                     /locus_tag="J8I47_001967"
     CDS             complement(9786..11540)
                     /locus_tag="J8I47_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323397.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11650..>12196)
                     /locus_tag="J8I47_001968"
     CDS             complement(11650..>12196)
                     /locus_tag="J8I47_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAZ6323398.1"
                     /translation="IGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVY
                     ISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQ
                     VASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLV
                     VASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3857 a         2383 c         1796 g         4160 t
ORIGIN      
        1 actacttcaa aagaatgcgt gggaattgaa tggtcagcat cgctactggc gtccgtcatt
       61 tcgagatcat ctgacagaag cggagatagt cgatcggcta ttatattatg atgactcctt
      121 aaagcgaggc tacgaagtct accaggtttt tctttccgct atcagaagac aagatgttcc
      181 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact atcaaccagt
      241 gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag ttccctattc
      301 caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg
      361 ctttagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa aatatttcaa
      421 gaaaactaag aacactaccc aactgactaa agccagaaca acaacacggc tagacaagat
      481 agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt
      541 gacgaagagc caaaataagt aatctaaccg actgttaact aaaccattgc aggtcccgtt
      601 aaagatactt gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc ttaacacact
      661 taataaagcc cctgctatta ttaataataa tatctttttc ttattaaaaa cctcctcatc
      721 tatttccaga ttagtaaatg tagtaatctg tgctttcttt tttctcaata ctaataacta
      781 acttatgggg taaacaaata ctcgtttgac cgggttttga gatccatcca gttctaaccg
      841 cgatttgatc aggactatta tcttctttaa ctcgaattct tccatcttta atctcaacaa
      901 tattatattg tcccttcgca ggatgataat tcacgagttt atgattgatc ttatccaaat
      961 cgaagcgatc catttccttg ccatcaattc gaacgatggc atatttgata gcatcttcag
     1021 ttgtttcatt ctccttttct atagagaata caaataaggg aataaaagag gtaataatca
     1081 aaatgccaat aattatgaca tcgcctatct tgacaccagc ttttcttaaa aatttcatta
     1141 acgatatctg gctaaacata ttagactttt tccatggaaa tggtgaaatt tttcggaaat
     1201 tatctgcatt aagagaatca gttaaacaac cctcaagttg ttccttaata cattgttgat
     1261 ccatcttcat accaataaaa actagtttat taatgcgttc accaaacaac ggatgccagg
     1321 cttccttcaa ttcgggattt ttttgtataa tcatttgaca ctcaatttta ggtaaactag
     1381 ctacccaata atttaatgat tcaatacttt tatagctacc cgatgtttca aagatcgtcg
     1441 aaaaatcact atgttgggca aaccaaagaa acccttttgc tcttgtaata ttgccaggaa
     1501 aatttttatc cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga tttcgataga
     1561 taaaacttga gataccatat tcttctgttt caggaatatg attttgatag ccaagctcta
     1621 actctttgaa ccagcccact gaataggaag tctcatctat attaaaaaga tttgtattta
     1681 gaattttcga taagtctact tcacctttaa ttgtcctaat tattttggca ttggactgta
     1741 acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag tcactattat
     1801 ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga atatcctgtt
     1861 cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc gtttcgaaat
     1921 tatcccaaaa acgttttaca tccactacta taataatggc accaatctct aatcgttggg
     1981 tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt ggaattggtt
     2041 tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc actaattgat
     2101 ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa
     2161 caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca tcaacattta
     2221 cttcccctat gtcatttaca atgacagcaa tttcctttcc gtgatcaccg tacagaatat
     2281 gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta ataggtattt
     2341 ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa
     2401 tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat
     2461 aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct ttctgcttca
     2521 cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat
     2581 tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc ggaaaaaaat
     2641 tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg
     2701 tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt
     2761 ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg tgcaatttgc
     2821 aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg
     2881 caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc
     2941 cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga ttttggtcat
     3001 tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac tttgacagac
     3061 agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg
     3121 agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact
     3181 ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt gaataatatg
     3241 cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata
     3301 gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga
     3361 taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac
     3421 cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag acataataaa
     3481 aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca attaattggc
     3541 caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca
     3601 acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc
     3661 agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc gataacgccg
     3721 ataagcatca atattcttgg aatccataga caataatact tttcttacca tgtatttcct
     3781 ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt agacaatgaa
     3841 gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg actttctcca
     3901 ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc actaaattga
     3961 atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg
     4021 tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt
     4081 tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc
     4141 ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt attgattttt
     4201 gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc
     4261 cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca
     4321 ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc
     4381 atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca
     4441 ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa ttttatccca
     4501 gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa gtaaatataa
     4561 ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga agatggctct
     4621 tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa agctaaatat
     4681 ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag actagtggta
     4741 tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact
     4801 tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt
     4861 gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg
     4921 tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact
     4981 acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt
     5041 tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc
     5101 tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg
     5161 acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca
     5221 tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga
     5281 gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg
     5341 ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc ctttttctag
     5401 ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta
     5461 gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt tatcaaaact
     5521 ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg tttggtcttt
     5581 ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg caactataaa
     5641 atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa
     5701 aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa
     5761 gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc cattatagtg
     5821 attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca aattataaaa
     5881 atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat
     5941 acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga
     6001 cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc tttcaataag
     6061 ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag
     6121 ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc
     6181 aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa
     6241 tattgctaat aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac
     6301 ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc
     6361 aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt
     6421 tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc
     6481 tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa
     6541 tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga
     6601 cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca
     6661 atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc
     6721 cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat
     6781 cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat
     6841 catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct
     6901 gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag
     6961 tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta
     7021 aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt
     7081 gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta
     7141 cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga
     7201 gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt
     7261 cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc
     7321 taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct
     7381 gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc
     7441 gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc atcgccattt
     7501 gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt
     7561 tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga
     7621 gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg
     7681 aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttagg
     7741 ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta
     7801 gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt
     7861 atgtttaact ggtttatcgc caatttttta aaactttgat tattccttat attataagaa
     7921 tagacattaa gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc
     7981 tgtttaataa aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt
     8041 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
     8101 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
     8161 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
     8221 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
     8281 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
     8341 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
     8401 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
     8461 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
     8521 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
     8581 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
     8641 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
     8701 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
     8761 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
     8821 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
     8881 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
     8941 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
     9001 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
     9061 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
     9121 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
     9181 tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt
     9241 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
     9301 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
     9361 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
     9421 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
     9481 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
     9541 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
     9601 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
     9661 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
     9721 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
     9781 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
     9841 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
     9901 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
     9961 gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac
    10021 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
    10081 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
    10141 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
    10201 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
    10261 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
    10321 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
    10381 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
    10441 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
    10501 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
    10561 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
    10621 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
    10681 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
    10741 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
    10801 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
    10861 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
    10921 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
    10981 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
    11041 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
    11101 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
    11161 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
    11221 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
    11281 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa
    11341 gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata
    11401 attagagact gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa
    11461 aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat
    11521 aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt
    11581 acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta
    11641 aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat
    11701 agataaactt gctacaacta acactgaaat gattccccat atacctaccg catagtggat
    11761 cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc
    11821 tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca
    11881 agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa
    11941 gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa
    12001 aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct
    12061 aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc
    12121 aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac
    12181 tgtagctaaa ccaata
//
LOCUS       DADOZM010000089        12172 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.092, whole genome shotgun sequence.
ACCESSION   DADOZM010000089 DADOZM010000000
VERSION     DADOZM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.092"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            592..1920
                     /locus_tag="J8I47_001969"
     CDS             592..1920
                     /locus_tag="J8I47_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAZ6323399.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="J8I47_001970"
     CDS             2122..2955
                     /locus_tag="J8I47_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAZ6323400.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="J8I47_001971"
     CDS             2971..3708
                     /locus_tag="J8I47_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAZ6323401.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4789)
                     /locus_tag="J8I47_001972"
     CDS             complement(3863..4789)
                     /locus_tag="J8I47_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAZ6323402.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5064..5897
                     /locus_tag="J8I47_001973"
     CDS             5064..5897
                     /locus_tag="J8I47_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAZ6323403.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5931..6770
                     /locus_tag="J8I47_001974"
     CDS             5931..6770
                     /locus_tag="J8I47_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6323404.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6796..7356
                     /locus_tag="J8I47_001975"
     CDS             6796..7356
                     /locus_tag="J8I47_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ6323405.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7555..9276
                     /locus_tag="J8I47_001976"
     CDS             7555..9276
                     /locus_tag="J8I47_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAZ6323406.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9441..10583
                     /locus_tag="J8I47_001977"
     CDS             9441..10583
                     /locus_tag="J8I47_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ6323407.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10599..11810
                     /gene="thiI"
                     /locus_tag="J8I47_001978"
     CDS             10599..11810
                     /gene="thiI"
                     /locus_tag="J8I47_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAZ6323408.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3455 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt attttttttc attttacaga cttccttacg aataattttg
     4861 catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag tgaagaataa
     4921 cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag actcaacctt
     4981 gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc agccaaggaa
     5041 atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt aaagaacatg
     5101 acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt ctacccttag
     5161 tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag aattggataa
     5221 gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag
     5281 gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt ttagatgtta
     5341 tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc ccctcatcat
     5401 tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg tttgagggtt
     5461 atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt cttgaacgta
     5521 caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag ccacagttgc
     5581 tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct ctttttcgat
     5641 tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt aaaaaaagaa
     5701 cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc aaagtagttg
     5761 attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt gatgcggctg
     5821 aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc aatccaaccc
     5881 aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct atgatcaaag
     5941 ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa
     6001 caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta gcaacggggc
     6061 gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg tatgtcgtgt
     6121 ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct tttgaacaaa
     6181 aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc gtttttggtg
     6241 cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt tttatcaagc
     6301 gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg
     6361 tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta aaagaaccaa
     6421 tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa catcgctttc
     6481 ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca aaaggtggtt
     6541 caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg gaagaaatta
     6601 tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga attggagtgg
     6661 ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact caatcaaatg
     6721 aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag tcaatgaaag
     6781 aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac attccacaaa
     6841 acttttgatc caagaagacc agagataccc acggctttta cagaaaatga agctcataca
     6901 cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc acctaataaa
     6961 gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc agaagaaaaa
     7021 atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga tgcactgaca
     7081 gatatccttt actttactta tggttctttt tcattactag gaatagtgcc tgatcgaata
     7141 ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa acctcattat
     7201 gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata tgctccagaa
     7261 cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa gaagaaatcc
     7321 gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta tttttgttat
     7381 ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat gaaaatagta
     7441 tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga tttatgccaa
     7501 gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc ataaatggga
     7561 aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt cgtctttttg
     7621 gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag
     7681 gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg
     7741 gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga catatctact
     7801 tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt
     7861 cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga ccaaatggag
     7921 aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga agaacgcaac
     7981 tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa
     8041 gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa agtgatcgaa
     8101 aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga agcccgcgag
     8161 gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa aaaaattcca
     8221 ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat cagtgatact
     8281 tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc tgaagatatc
     8341 gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa
     8401 acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata tgatatctta
     8461 gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat tgcagagtat
     8521 atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca cacatctcaa
     8581 agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa
     8641 gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga aattccttat
     8701 tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa
     8761 caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa
     8821 aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga aaaacaacgt
     8881 ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga ccgtattgaa
     8941 gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt
     9001 gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt agtagatgca
     9061 gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac acacgaagaa
     9121 gtgagagaag caattgataa aagctactat ttattcaata aagaatatca ctatcaagaa
     9181 gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt caaacgcatc
     9241 gaagattttt attttaatca tccagactta gtataaagca aaaaagttag taaaccagca
     9301 agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa aattgaaaca
     9361 agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata gaaaagaatt
     9421 tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc ccaatctttc
     9481 cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat ccatcaagct
     9541 tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag cagatcgctg
     9601 atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca gaaggagata
     9661 actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat atgatcattt
     9721 caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg
     9781 aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat
     9841 taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa gtaggatcga
     9901 ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt catttccatg
     9961 tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca gaaagagttg
    10021 attttgcaac attttcagct cataaattcc atggcccacg gggaactggt ttcatttatt
    10081 ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa agaaatcagc
    10141 gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta cgtttatcat
    10201 tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct ctaatcaatg
    10261 cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat gcaccgcata
    10321 ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa
    10381 aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg gcaagcagta
    10441 cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga atcagcttag
    10501 atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag cagctttata
    10561 agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt gaaattatgg
    10621 tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc atgcaactag
    10681 cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat gctgatcggg
    10741 accggatgca tcttttattg aatggcgcag acgggcagct agttattcca aaactagcaa
    10801 aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt
    10861 tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa acatttaaaa
    10921 ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg aatcaaacat
    10981 taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt caaccagata
    11041 ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg
    11101 gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt tctgggggga
    11161 ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt gaagctgttc
    11221 attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa gatctaacag
    11281 ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt accgagattc
    11341 aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact cgtcgaatga
    11401 tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg
    11461 aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa
    11521 cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc attgaagtag
    11581 cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc tgtacgattt
    11641 ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa tatgaagagc
    11701 gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg gaagagatac
    11761 aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa gaggttatga
    11821 aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc catgtatttt
    11881 accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca agaagttatg
    11941 gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga tcaagctaca
    12001 gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt gacagaagtg
    12061 ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt ttgtgaaatt
    12121 tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt tg
//
LOCUS       DADOZM010000090        12171 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.093, whole genome shotgun sequence.
ACCESSION   DADOZM010000090 DADOZM010000000
VERSION     DADOZM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.093"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(64..621)
                     /gene="frr"
                     /locus_tag="J8I47_001979"
     CDS             complement(64..621)
                     /gene="frr"
                     /locus_tag="J8I47_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAZ6323409.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(624..1346)
                     /locus_tag="J8I47_001980"
     CDS             complement(624..1346)
                     /locus_tag="J8I47_001980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAZ6323410.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1482..2363)
                     /locus_tag="J8I47_001981"
     CDS             complement(1482..2363)
                     /locus_tag="J8I47_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAZ6323411.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2462..3244)
                     /gene="rpsB"
                     /locus_tag="J8I47_001982"
     CDS             complement(2462..3244)
                     /gene="rpsB"
                     /locus_tag="J8I47_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAZ6323412.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3603..4082
                     /locus_tag="J8I47_001983"
     CDS             3603..4082
                     /locus_tag="J8I47_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAZ6323413.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4299..5390
                     /gene="phnW"
                     /locus_tag="J8I47_001984"
     CDS             4299..5390
                     /gene="phnW"
                     /locus_tag="J8I47_001984"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAZ6323414.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5383..5511
                     /locus_tag="J8I47_001985"
     CDS             5383..5511
                     /locus_tag="J8I47_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAZ6323415.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5515..6060
                     /locus_tag="J8I47_001986"
     CDS             5515..6060
                     /locus_tag="J8I47_001986"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAZ6323416.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6514..8205)
                     /locus_tag="J8I47_001987"
     CDS             complement(6514..8205)
                     /locus_tag="J8I47_001987"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAZ6323417.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8625..9572)
                     /gene="arcC"
                     /locus_tag="J8I47_001988"
     CDS             complement(8625..9572)
                     /gene="arcC"
                     /locus_tag="J8I47_001988"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAZ6323418.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9687..10706)
                     /gene="argF"
                     /locus_tag="J8I47_001989"
     CDS             complement(9687..10706)
                     /gene="argF"
                     /locus_tag="J8I47_001989"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAZ6323419.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10797..12026)
                     /gene="arcA"
                     /locus_tag="J8I47_001990"
     CDS             complement(10797..12026)
                     /gene="arcA"
                     /locus_tag="J8I47_001990"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAZ6323420.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3333 a         2527 c         2202 g         4109 t
ORIGIN      
        1 aaaagaaata taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt
       61 ttattaaact tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttgat
      121 gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat
      181 atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat
      241 cgcgattttt gcattttctg cttctttttt aacatcttta gctaattctt ttctgcgttc
      301 ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc
      361 gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt
      421 gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg
      481 cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg
      541 gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc
      601 tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct
      661 aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca
      721 ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact
      781 tccagatgag tcagttcttc aaattttact gcatttgcat ctactttcgg atcagcagaa
      841 taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgcg
      901 cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata
      961 acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt
     1021 tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc
     1081 tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt
     1141 tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccgcc cacaacgatt
     1201 gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact
     1261 ggtggtttga ttccaaatcc tgcgtcccca gctaaagctt ctccactcaa ttttaagact
     1321 acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta
     1381 ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg
     1441 gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc
     1501 ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt
     1561 acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga
     1621 tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct
     1681 tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga
     1741 acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga
     1801 ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct
     1861 agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg
     1921 tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt
     1981 gcttcgatca agtcagattc aattgtacct ttatctgttt tgattttcat tgcagcttcc
     2041 atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac
     2101 atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta
     2161 cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc
     2221 tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca
     2281 actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc
     2341 attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa
     2401 gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa
     2461 attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg
     2521 ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga
     2581 tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg
     2641 aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg
     2701 ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt
     2761 acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt
     2821 cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt
     2881 cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg
     2941 tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt
     3001 ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt
     3061 cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt
     3121 caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc
     3181 gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg
     3241 ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg
     3301 caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa
     3361 gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg
     3421 ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct
     3481 ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta
     3541 ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga
     3601 aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc
     3661 gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa
     3721 cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt
     3781 cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc
     3841 ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga
     3901 tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac
     3961 ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg
     4021 aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt
     4081 aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta
     4141 ttgaataaac ctttacactc tttacaaaaa tacatcattt tttattcaaa tttttcaaca
     4201 ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc
     4261 tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaacttctg
     4321 gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg
     4381 atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat cttgctcaag
     4441 tatcggaagg aatatacaca acggtcttaa tgcaaggtag cagctccttc ggggtagaat
     4501 cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat
     4561 atagacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg
     4621 tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccgt
     4681 ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg
     4741 aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac gcgatgtcaa
     4801 gctttggtgg gataccgatt tgtgtagccg aactagggat tgattttttg atttccagct
     4861 caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat aaagcggaac
     4921 tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac cagtaccgcg
     4981 tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt
     5041 ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt
     5101 atctcgccaa taatcatgtg ctacgacaaa aaatgacaga gatggggttc cagcctttca
     5161 ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg
     5221 attttaatca tttttatcag tatttaaagc aaaatggatt tgtgatctat cccgggaaaa
     5281 tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt
     5341 tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt
     5401 tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga
     5461 aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcta accgatggga
     5521 aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg
     5581 atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt
     5641 gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa
     5701 acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgaatgg atttaatgct
     5761 cctatgatga caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc agacttcatg
     5821 gcaacttttg aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac
     5881 atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca
     5941 gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg
     6001 ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa
     6061 aacagtctgt tgctcttaga ttccaacaag ccggcgctga tatttgtgta aacaatttaa
     6121 aagagttaac agaatacatt ttgtacgctg acatgaatcc agaatgactt ttatacaaaa
     6181 taagaggttg tgaaaaagct gtcctgtatc aagtaataaa aacagccaag caaaaatagc
     6241 tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
     6301 tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttgcaaa
     6361 caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt
     6421 atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc
     6481 ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc
     6541 tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc
     6601 ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg
     6661 tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa
     6721 gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa
     6781 tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg
     6841 agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag
     6901 gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac
     6961 cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg
     7021 ttctttagca gattggattg cttcatttaa tacttcttct aataagacga tctttccttt
     7081 acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat ggtgcatgtc
     7141 gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct
     7201 ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa
     7261 tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc
     7321 tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa
     7381 atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc
     7441 gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga
     7501 ttcatcacgg aaccattgcc ataattgaat agcctcttta tcgccttctt ccagacgttt
     7561 gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg
     7621 gacgtataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca
     7681 ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat
     7741 gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt
     7801 cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg
     7861 tactgttcct tgattcccga tgttgctatc cccataatgt tctttttctt tcactactgt
     7921 ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac
     7981 aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg
     8041 ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg
     8101 ttcagctgat tttggatttt ctagtaattg ctcgacttgt tccagtgtta aatcatcttt
     8161 gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa
     8221 agtgttccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat
     8281 cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca
     8341 tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta
     8401 tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc
     8461 tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg
     8521 aaaggctagt cgtttcataa aaaaacaatc cagatggcct aaaggagaag aaataccacc
     8581 tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc agctacgata
     8641 atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt gatgattgcc
     8701 ttagcgtttg gttttgcttc aacaaatgca atagctgctt ctactttcgg aagcatgctt
     8761 cctggagcga attgcttttc atcaatgtat tgtttcattt ctgaaacagt aacagtttct
     8821 aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc tgtcaatacg
     8881 atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc tttatcgatt
     8941 acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc accgccacca
     9001 acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc
     9061 gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc ttctttgaat
     9121 gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta gaatggaccg
     9181 actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac ttgagtgact
     9241 aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat ggcgttttgt
     9301 aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg cattgccggt
     9361 gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg cggaccattt
     9421 ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata ctttgctgtt
     9481 tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat ggcattgccc
     9541 cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc agttctaccg
     9601 aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg ctgactcgtt
     9661 ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt acctaatgtt
     9721 gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa ttgacgagca
     9781 tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa gcgttctttc
     9841 atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg caagaagatc
     9901 aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta aggtttcaac
     9961 aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac atcagaataa
    10021 aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa ttgtgcaccg
    10081 gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg taattctttt
    10141 ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa agagttagcc
    10201 atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt tccaaagttt
    10261 tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc agtcaagcca
    10321 ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg actgaatcca
    10381 cggaattcga ttccgtcaaa catacgtcct agtactttag ctgtatcttc tgtggattct
    10441 tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc tagatcgatc
    10501 gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa aagcgcaata
    10561 ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt caagtgttct
    10621 gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc taataagctt
    10681 cttccttgga atactgattc tttcatatta aacattctcc tttagattgt ttttatttaa
    10741 gattaaagag gaaatcccga cggcagctga cagtaagact gccgtcagga aacaaattac
    10801 ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg acctcgtgac
    10861 aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa ttcgttcgat
    10921 acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt gtttgatcca
    10981 tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg gatcagtaca
    11041 agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga atgagtaatc
    11101 ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc tttactttga
    11161 attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt atccaaatgc
    11221 atcattgcac ggttgttcgg aattttgatt gccaacactt tttcaaatcc tgaatttttt
    11281 gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg agaaatccca
    11341 acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg atctggattt
    11401 tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg tttcaaaata
    11461 tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt gactgtcatc
    11521 ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat tgggtccatg
    11581 taaaatggat agtcatcgtc tgctgacaga tcatatagac tgctagaacg tacttcgata
    11641 tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc cattgataga
    11701 aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc atccaacatt
    11761 ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc taatttttcc
    11821 aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc atgttcttct
    11881 tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat atctggtgtc
    11941 aaattttcca cttcttgtcc aggccgtttc agtaaaactg tctttaattt ccctatttca
    12001 gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga ttacatgtat
    12061 aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat gagacattcc
    12121 tcacattaat attcgataat taaataatta aacgtttttc tatttttaag c
//
LOCUS       DADOZM010000091        11939 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.094, whole genome shotgun sequence.
ACCESSION   DADOZM010000091 DADOZM010000000
VERSION     DADOZM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.094"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(5..586)
                     /locus_tag="J8I47_001991"
     CDS             complement(5..586)
                     /locus_tag="J8I47_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323421.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="J8I47_001992"
     CDS             complement(769..1395)
                     /locus_tag="J8I47_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ6323422.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="J8I47_001993"
     CDS             complement(1417..2748)
                     /locus_tag="J8I47_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAZ6323423.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="J8I47_001994"
     CDS             complement(2903..3304)
                     /locus_tag="J8I47_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ6323424.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="J8I47_001995"
     CDS             complement(3390..3596)
                     /locus_tag="J8I47_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323425.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="J8I47_001996"
     CDS             complement(3691..5193)
                     /locus_tag="J8I47_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAZ6323426.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="J8I47_001997"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="J8I47_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAZ6323427.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="J8I47_001998"
     CDS             complement(5764..6381)
                     /locus_tag="J8I47_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323428.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="J8I47_001999"
     CDS             6602..7975
                     /locus_tag="J8I47_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ6323429.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="J8I47_002000"
     CDS             8050..9162
                     /locus_tag="J8I47_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAZ6323430.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="J8I47_002001"
     CDS             complement(9234..10871)
                     /locus_tag="J8I47_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAZ6323431.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="J8I47_002002"
     CDS             complement(11039..11647)
                     /locus_tag="J8I47_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6323432.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3172 a         2493 c         2148 g         4126 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actagttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tggtttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gactctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggtctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaa
//
LOCUS       DADOZM010000092        11677 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.095, whole genome shotgun sequence.
ACCESSION   DADOZM010000092 DADOZM010000000
VERSION     DADOZM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.095"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(135..1046)
                     /locus_tag="J8I47_002003"
     CDS             complement(135..1046)
                     /locus_tag="J8I47_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ6323433.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1449..2027)
                     /locus_tag="J8I47_002004"
     CDS             complement(1449..2027)
                     /locus_tag="J8I47_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323434.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2047..2715)
                     /locus_tag="J8I47_002005"
     CDS             complement(2047..2715)
                     /locus_tag="J8I47_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323435.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2730..3800)
                     /locus_tag="J8I47_002006"
     CDS             complement(2730..3800)
                     /locus_tag="J8I47_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323436.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3971..5335)
                     /locus_tag="J8I47_002007"
     CDS             complement(3971..5335)
                     /locus_tag="J8I47_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323437.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5332..6891)
                     /locus_tag="J8I47_002008"
     CDS             complement(5332..6891)
                     /locus_tag="J8I47_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323438.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6947..9427)
                     /locus_tag="J8I47_002009"
     CDS             complement(6947..9427)
                     /locus_tag="J8I47_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323439.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9411..9746)
                     /locus_tag="J8I47_002010"
     CDS             complement(9411..9746)
                     /locus_tag="J8I47_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323440.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9760..10794)
                     /locus_tag="J8I47_002011"
     CDS             complement(9760..10794)
                     /locus_tag="J8I47_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAZ6323441.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10884..11564)
                     /locus_tag="J8I47_002012"
     CDS             complement(10884..11564)
                     /locus_tag="J8I47_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ6323442.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         3343 a         2386 c         2025 g         3923 t
ORIGIN      
        1 tccttgtgac gcggagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa
       61 gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta tttttgtttg
      121 gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact tgcagcaatc
      181 tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt catataaccc
      241 atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt cccagtggag
      301 attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg tgctaatgtt
      361 tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc atattccgta
      421 gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta gtcgcatata
      481 tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc tccagcatcc
      541 tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc ttctgtttgt
      601 tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa tacaatcact
      661 gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt tccatcttcg
      721 agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt ttttgtttca
      781 gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt tagcgagtca
      841 tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg ttctgtatca
      901 tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt ttgtccaact
      961 atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat gattgcaaat
     1021 acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc agactaaggg
     1081 tcaaaactta aaatcagctt aagtacgcaa aaaaggggct gtaaaagata taaccgtaga
     1141 aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa atctcctttt
     1201 gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac aaacaattaa
     1261 tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat aaaaatatac
     1321 cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta tataaaaaaa
     1381 acaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt tctccctata
     1441 taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa ttgcgccaca
     1501 tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa cactaagaaa
     1561 aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg gtttgctgaa
     1621 aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag atgatctctt
     1681 gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc gaccagatgt
     1741 gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt ttcgcttgtt
     1801 tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa aactgccgta
     1861 tatagttttt ctttatcacc gaaataatga tagagattgg gttgggtgat cccaactttt
     1921 tgtgcgatct ctcttgttga agtgccgaga tatcccttgg acataaacaa gtcagtcgct
     1981 tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt tccacctgct
     2041 ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc tatcgcattt
     2101 gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt cttttgattc
     2161 ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag cagttggttc
     2221 atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga ccctttgccg
     2281 ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc caagctgctc
     2341 aaataattct tttgcttctt tttgacgatg ttcatgtttg atcttatcta ctaataataa
     2401 ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa agccaatttc
     2461 tttaaaacgt agtttaacgc gttcactttc tttttgatta ctgaattctt gatcattgat
     2521 aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa aagtactttt
     2581 acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac tcatggttgt
     2641 ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta cttctttaaa
     2701 ttctattgct ttcatgctct tcctccttct tatccaattg ccttcaacgg atcgattttt
     2761 acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctaccagcat caacaagcta
     2821 atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag aacaagggat
     2881 gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat gaatgtttgt
     2941 gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat tacaccaaag
     3001 atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat agccgcaata
     3061 acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata tccaggtaat
     3121 tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt actgatttta
     3181 cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt ttcgaagcgg
     3241 atctcttggt aagcattgat ggttgtatag agaactggtg agacattgaa tttggcatga
     3301 tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac tgtatctcct
     3361 acggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc atcgttatca
     3421 aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat accgaagaag
     3481 ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc aggtgtctgg
     3541 gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt aatcatggac
     3601 atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc ccacttgtcg
     3661 actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa aaataccagg
     3721 taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga atgcataatt
     3781 tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta tacaacaatt
     3841 ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg ctatttcatt
     3901 atatgaattt tttattctcg attgattaat atttattata tccccacaca ataaaagacc
     3961 gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta gctagtactt
     4021 taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga aatccaaaat
     4081 cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca agcaggttca
     4141 aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct aatgtatccg
     4201 gatggtagat cagctttgca tttactgttt tgttttcttc atcgtattgt ggaaatagat
     4261 caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca tcttctgtca
     4321 gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt tgtctgaggt
     4381 cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata tgtacagctc
     4441 gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact gggacgatat
     4501 ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact atcacagtct
     4561 catcctcata caaagccact ttttctttta acaatgtcgt attaggacga gtttgggcag
     4621 aaaaaatgac catatctgcc tttattgctt gagatagaag ttgtacggat actactttct
     4681 cttctgattt tttccaatcg atcaagaatt catttggata aaaattgatt tgcttagttt
     4741 gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat aaaacattcg
     4801 gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct gctaattcca
     4861 atccaatata accagcaccg aagatcacta cttcttttgc ttcttttaaa gcggcaagta
     4921 cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca atttgtttat
     4981 acaaggtgac aggagaggaa cctgttgcta cgattaattt atcataggtt atggtctcgt
     5041 tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt tcagttactc
     5101 tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta tgggcaactt
     5161 cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa acatagccta
     5221 gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga agttttctta
     5281 gatttagggc agctgtgatg cctgcatggg aagcaccaat gatcacgatt ttcatgtttt
     5341 attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt tcaaataggg
     5401 gaaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact tctgtgacgg
     5461 gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct cgtattcgac
     5521 taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca cgatatccgc
     5581 cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta actgcttgat
     5641 cagcgtccgc aaaatagatg catagcgagc aaataattca aagcgggcat tttcctcgtc
     5701 tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac ataaaaccag
     5761 gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc caaaaatatt
     5821 catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca acgcaatctt
     5881 ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg attgtttaaa
     5941 cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca aaaactcctg
     6001 gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat aaaaaaccaa
     6061 cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca aaaatgtatg
     6121 gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc tataatctgg
     6181 tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata tttcaaattt
     6241 caatcgtcct tcttctgtaa tagtaaaact aaactctttt tgcagcagat ctaaaaaagt
     6301 gtttcctgat aaatcactgt attctttttt gttttcaaga taaggacgtc caaaccaaaa
     6361 taattggaaa taaaaataac aaatttgact ttctggaccg gtcagccgcc cgctttttat
     6421 tttcagatga aactcattta aagaatcatt taattccttc actctgcgag acaaagtagc
     6481 cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat tttccaacag
     6541 ataaagaagt atctgatatt ttgtagaata gcgcaagtag caatgataat aaacatcgac
     6601 tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa caatttccaa
     6661 ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca aggtagcccg
     6721 atcaactgat aagcgggtaa gtacgttatt aacggaaatc tctccttttt cctctaataa
     6781 aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga aaagttccaa
     6841 gtccactcct ccaagcattt ctgattttaa cagcagaagt ttccttctca tccttgaaaa
     6901 agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag ctggttggcc
     6961 cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga gcattcgtac
     7021 tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga acctctcctt
     7081 tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc aattgttttg
     7141 atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga tcattcttaa
     7201 ataaagaagt caatgtccct gtcagttgcc aaggagacgt gcttggaagc cgagtatccc
     7261 gcacatcaaa agaaaaatcg ccttttgctt cataataact tgtcttcaac ggtttgggga
     7321 gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct tgtttgggga
     7381 caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca ccatctgcta
     7441 atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat tgtacagaag
     7501 tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct attttgctca
     7561 atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata gttgtaccat
     7621 ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc gtgatttttt
     7681 tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct tgaatcgtat
     7741 ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg ttttttagct
     7801 gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga gtggtgattc
     7861 cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta agtacatcgt
     7921 ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt gtagaagtag
     7981 ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct gtccatcctg
     8041 tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc ttttttagtt
     8101 cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc tttccccact
     8161 gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac tgctccaata
     8221 cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg tacacgatat
     8281 tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt gctccttgtg
     8341 taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat ggcaactgat
     8401 caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag cctggaaggt
     8461 tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat tttcctgttg
     8521 aatcacttgt tgagatgtaa ctattccacg tatttatctc tgcacttgca gaaaatgcat
     8581 atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct gcagctggag
     8641 ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa aaatcaacag
     8701 aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa attttgtttg
     8761 gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct gtaacatcaa
     8821 attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca ataatatctg
     8881 ccaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg cttcctgctg
     8941 ataataaact accgctagta accttgatgg gggcaccggc aatatacaag taggcgttgt
     9001 tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa ttgatgatcg
     9061 ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt gttagtttca
     9121 catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt gaaccatcgt
     9181 tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg acaagcttcc
     9241 cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac gtgtatggat
     9301 cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa aagataaata
     9361 tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat ttattttgta
     9421 gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc aagaagccat
     9481 aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt ctccgggaat
     9541 acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt tgatgcgcca
     9601 ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat cgtacctgaa
     9661 cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact tgcaataatc
     9721 cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt ctcctttttt
     9781 ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa ctgcagcaac
     9841 caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg aattttcatt
     9901 tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc attcattact
     9961 gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt tatcaccagc
    10021 taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat tagacgtatt
    10081 ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat tgctgataat
    10141 cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga cctcgtccaa
    10201 atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt gtaaaacgac
    10261 tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat tttctgtttc
    10321 agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa agctttttgc
    10381 tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat tgatttcctt
    10441 tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta acgttgggtt
    10501 tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga cagagacctc
    10561 aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct gattttgttc
    10621 cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct ctggaaatac
    10681 cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg gaaaaatgaa
    10741 aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat tcattgtcca
    10801 ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat tttcaccgca
    10861 gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt ccaagtcaaa
    10921 tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt tgctggatca
    10981 taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat tggtgtcgct
    11041 gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct
    11101 gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa
    11161 ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca
    11221 cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact
    11281 tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc aggaactaca
    11341 tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc tggctctact
    11401 ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc agtaaaggta
    11461 atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga
    11521 agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt
    11581 ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc
    11641 ttacttgaaa ttatgaccct ttttgtataa aagagaa
//
LOCUS       DADOZM010000093        11595 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.096, whole genome shotgun sequence.
ACCESSION   DADOZM010000093 DADOZM010000000
VERSION     DADOZM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.096"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            107..970
                     /locus_tag="J8I47_002013"
     CDS             107..970
                     /locus_tag="J8I47_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ6323443.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="J8I47_002014"
     CDS             complement(1099..2721)
                     /locus_tag="J8I47_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6323444.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="J8I47_002015"
     CDS             2970..3491
                     /locus_tag="J8I47_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323445.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="J8I47_002016"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="J8I47_002016"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAZ6323446.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="J8I47_002017"
     CDS             5572..6390
                     /locus_tag="J8I47_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAZ6323447.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="J8I47_002018"
     CDS             complement(6493..6690)
                     /locus_tag="J8I47_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323448.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="J8I47_002019"
     CDS             6859..7437
                     /locus_tag="J8I47_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAZ6323449.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="J8I47_002020"
     CDS             complement(8482..8985)
                     /locus_tag="J8I47_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAZ6323450.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="J8I47_002021"
     CDS             9344..10369
                     /locus_tag="J8I47_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ6323451.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="J8I47_002022"
     CDS             10692..11570
                     /locus_tag="J8I47_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323452.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3591 a         1973 c         2258 g         3773 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt ttaaa
//
LOCUS       DADOZM010000094        11524 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.097, whole genome shotgun sequence.
ACCESSION   DADOZM010000094 DADOZM010000000
VERSION     DADOZM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.097"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(220..705)
                     /locus_tag="J8I47_002023"
     CDS             complement(220..705)
                     /locus_tag="J8I47_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAZ6323453.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1185..1826
                     /locus_tag="J8I47_002024"
     CDS             1185..1826
                     /locus_tag="J8I47_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ6323454.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1845..2480
                     /locus_tag="J8I47_002025"
     CDS             1845..2480
                     /locus_tag="J8I47_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323455.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2498..3313
                     /locus_tag="J8I47_002026"
     CDS             2498..3313
                     /locus_tag="J8I47_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6323456.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3632..5068
                     /locus_tag="J8I47_002027"
     CDS             3632..5068
                     /locus_tag="J8I47_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6323457.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5257..7704
                     /locus_tag="J8I47_002028"
     CDS             5257..7704
                     /locus_tag="J8I47_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAZ6323458.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7934..9001
                     /gene="rlmN"
                     /locus_tag="J8I47_002029"
     CDS             7934..9001
                     /gene="rlmN"
                     /locus_tag="J8I47_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAZ6323459.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9194..10297)
                     /locus_tag="J8I47_002030"
     CDS             complement(9194..10297)
                     /locus_tag="J8I47_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ6323460.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10850..11398
                     /gene="aac(6')"
                     /locus_tag="J8I47_002031"
     CDS             10850..11398
                     /gene="aac(6')"
                     /locus_tag="J8I47_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAZ6323461.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3826 a         1948 c         2484 g         3266 t
ORIGIN      
        1 ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat
       61 aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag
      121 ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa
      181 aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg
      241 cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt
      301 ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac
      361 gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg
      421 aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa
      481 ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat
      541 ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa
      601 agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag
      661 tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc
      721 tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac
      781 acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag
      841 tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc
      901 tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac
      961 cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac
     1021 gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg
     1081 aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg
     1141 gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg
     1201 aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct
     1261 tgattggctc attaccatta gggattgttt tagcattagg attgtctttg cattttaagc
     1321 cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc
     1381 aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag
     1441 cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag
     1501 ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct
     1561 ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag
     1621 ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg
     1681 acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag
     1741 gcgtggcaat catctatctt acattgactg gtattctaac aatcataatg aaacaagttg
     1801 aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga
     1861 aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag
     1921 atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta
     1981 tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca
     2041 acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc
     2101 tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac
     2161 agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg
     2221 atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg
     2281 ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag
     2341 atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa
     2401 cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa
     2461 aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt
     2521 cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga
     2581 taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt
     2641 tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa
     2701 agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa
     2761 ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc
     2821 ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat
     2881 cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc
     2941 gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat
     3001 agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa
     3061 aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca
     3121 aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc
     3181 agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca
     3241 actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac
     3301 gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga
     3361 ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc
     3421 tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat
     3481 gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa
     3541 cctcctttac ataattttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa
     3601 cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc
     3661 aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga
     3721 agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa
     3781 aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt
     3841 aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc
     3901 taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga
     3961 attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc
     4021 attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta
     4081 tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga
     4141 acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga
     4201 cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa
     4261 taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa
     4321 ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat
     4381 ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt
     4441 cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc
     4501 tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc
     4561 attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga
     4621 aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg
     4681 ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta
     4741 ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga
     4801 taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa
     4861 agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt
     4921 gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga
     4981 aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact
     5041 gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg
     5101 atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag
     5161 ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt
     5221 ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact
     5281 aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg
     5341 acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt
     5401 tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc
     5461 ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac agagttttca
     5521 attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat cttattattg
     5581 ttacatgccc atttatttgg caaactccat actggggaac caaatatcat ggggacgaca
     5641 tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg
     5701 atgattggag caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat
     5761 ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat
     5821 cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat
     5881 cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa
     5941 gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat
     6001 gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca
     6061 gaaaatacag agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc
     6121 catcctgcga atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa
     6181 ggtttttcag atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca
     6241 gaacaggaaa atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact
     6301 gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact
     6361 ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca
     6421 aaattcgaag tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat
     6481 gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa
     6541 tcactgattg gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc
     6601 atcgaagcac aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt
     6661 tctggacggg tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg
     6721 actggtagtg ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct
     6781 aagccccatg aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac
     6841 aacggcatac ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc
     6901 ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta
     6961 agaaatattt ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa
     7021 catccaacct taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta
     7081 gccagcaatg aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg
     7141 attcatatga tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa
     7201 gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc
     7261 attgattcta atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg
     7321 ggtgagaata aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg
     7381 gtcgttcaat ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca
     7441 gatgaagttg aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca
     7501 aaagcacttg tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg
     7561 atcggttaca accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc
     7621 ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg
     7681 ttgccggacg ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca
     7741 ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa
     7801 tacggtatga tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt
     7861 gtgaagtatt gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa
     7921 gagggaaaat actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa
     7981 ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta
     8041 tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa
     8101 attatcggag cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga
     8161 tggtacagta aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat
     8221 gagacaagaa tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg
     8281 tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt
     8341 tgcacagatc atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc
     8401 acatgtcgtt gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt
     8461 tttgcatatc atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc
     8521 cactagtgga ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct
     8581 ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg
     8641 aagtttccca attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg
     8701 tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca
     8761 acaattagca gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata
     8821 taatccagtc agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt
     8881 ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga
     8941 tattgatgca gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata
     9001 atcgtatcct actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac
     9061 tgttcaactt aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa
     9121 gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg
     9181 ttttagcaat tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat
     9241 aagtttctac gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat
     9301 gtttaatatc atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat
     9361 tgagattcat gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt
     9421 tatcaaacaa atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag
     9481 atgctccgtg aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt
     9541 gtgccatagc agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa
     9601 tgatatccga aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca
     9661 atgcttgcgt accataagtc aagagacgat caatgcacat ttttccgatt tggattccta
     9721 atctatccgt gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg
     9781 ataaagcatc gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg
     9841 tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt
     9901 cttcgttata gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa
     9961 aggtgaagac tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc
    10021 caccaccgac tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt
    10081 caatatttcc ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct
    10141 gtgtcaaagc tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca
    10201 gcactacttt tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc
    10261 caatggtata atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt
    10321 gaaacgggtt aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg
    10381 tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata
    10441 agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc
    10501 ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat
    10561 taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc
    10621 aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc
    10681 tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat
    10741 aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc
    10801 gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag
    10861 tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac
    10921 ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga
    10981 acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc
    11041 tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa
    11101 gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg
    11161 aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga
    11221 cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta
    11281 tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg
    11341 ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata
    11401 aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta
    11461 cttcttgaat ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt
    11521 gtac
//
LOCUS       DADOZM010000095        11181 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.098, whole genome shotgun sequence.
ACCESSION   DADOZM010000095 DADOZM010000000
VERSION     DADOZM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.098"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(183..1031)
                     /locus_tag="J8I47_002032"
     CDS             complement(183..1031)
                     /locus_tag="J8I47_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6323462.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="J8I47_002033"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="J8I47_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAZ6323463.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="J8I47_002034"
     CDS             2099..3100
                     /locus_tag="J8I47_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAZ6323464.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="J8I47_002035"
     CDS             3097..3381
                     /locus_tag="J8I47_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ6323465.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="J8I47_002036"
     CDS             3414..4211
                     /locus_tag="J8I47_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ6323466.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="J8I47_002037"
     CDS             4225..5058
                     /locus_tag="J8I47_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323467.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="J8I47_002038"
     CDS             5076..5933
                     /locus_tag="J8I47_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6323468.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="J8I47_002039"
     CDS             5955..6464
                     /locus_tag="J8I47_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323469.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="J8I47_002040"
     CDS             6501..6902
                     /locus_tag="J8I47_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323470.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="J8I47_002041"
     CDS             7014..7367
                     /locus_tag="J8I47_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323471.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="J8I47_002042"
     CDS             7401..7844
                     /locus_tag="J8I47_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ6323472.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="J8I47_002043"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="J8I47_002043"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ6323473.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="J8I47_002044"
     CDS             8939..9652
                     /locus_tag="J8I47_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6323474.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10404..10604
                     /locus_tag="J8I47_002045"
     CDS             10404..10604
                     /locus_tag="J8I47_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6323475.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3743 a         1748 c         2413 g         3277 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10261 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10321 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10381 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10441 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10501 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10561 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10621 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat
    10681 gggctgaata gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa
    10741 aatacgaata aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct
    10801 tgaaaatgaa tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg
    10861 gtctgtcaat taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta
    10921 aatttttaag taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg
    10981 aaggaatacg aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa
    11041 gtgatgaaag aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa
    11101 taattttttt attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt
    11161 taaaaataac tgtgttatat a
//
LOCUS       DADOZM010000096        10817 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.099, whole genome shotgun sequence.
ACCESSION   DADOZM010000096 DADOZM010000000
VERSION     DADOZM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.099"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(25..144)
                     /locus_tag="J8I47_002046"
     CDS             complement(25..144)
                     /locus_tag="J8I47_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAZ6323476.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="J8I47_002047"
     CDS             complement(159..1343)
                     /locus_tag="J8I47_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ6323477.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="J8I47_002048"
     CDS             complement(1599..1853)
                     /locus_tag="J8I47_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323478.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="J8I47_002049"
     CDS             complement(1878..3020)
                     /locus_tag="J8I47_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAZ6323479.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="J8I47_002050"
     CDS             complement(3080..4432)
                     /locus_tag="J8I47_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ6323480.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="J8I47_002051"
     CDS             complement(4503..4847)
                     /locus_tag="J8I47_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAZ6323481.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="J8I47_002052"
     CDS             complement(4857..5231)
                     /locus_tag="J8I47_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6323482.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="J8I47_002053"
     CDS             complement(5244..5558)
                     /locus_tag="J8I47_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ6323483.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="J8I47_002054"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="J8I47_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="J8I47_002055"
     CDS             complement(8976..9638)
                     /locus_tag="J8I47_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323484.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="J8I47_002056"
     CDS             10255..10485
                     /locus_tag="J8I47_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323485.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="J8I47_002057"
     CDS             10491..10790
                     /locus_tag="J8I47_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323486.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DADOZM010000097        10783 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.100, whole genome shotgun sequence.
ACCESSION   DADOZM010000097 DADOZM010000000
VERSION     DADOZM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.100"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            176..1435
                     /gene="lysA"
                     /locus_tag="J8I47_002058"
     CDS             176..1435
                     /gene="lysA"
                     /locus_tag="J8I47_002058"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAZ6323487.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1422..2222
                     /gene="dapF"
                     /locus_tag="J8I47_002059"
     CDS             1422..2222
                     /gene="dapF"
                     /locus_tag="J8I47_002059"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAZ6323488.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2225..3106
                     /locus_tag="J8I47_002060"
     CDS             2225..3106
                     /locus_tag="J8I47_002060"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAZ6323489.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3103..3819
                     /locus_tag="J8I47_002061"
     CDS             3103..3819
                     /locus_tag="J8I47_002061"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAZ6323490.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3825..4520
                     /gene="dapD"
                     /locus_tag="J8I47_002062"
     CDS             3825..4520
                     /gene="dapD"
                     /locus_tag="J8I47_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAZ6323491.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4536..5537
                     /locus_tag="J8I47_002063"
     CDS             4536..5537
                     /locus_tag="J8I47_002063"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAZ6323492.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5534..6895
                     /locus_tag="J8I47_002064"
     CDS             5534..6895
                     /locus_tag="J8I47_002064"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAZ6323493.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6855..8006
                     /locus_tag="J8I47_002065"
     CDS             6855..8006
                     /locus_tag="J8I47_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAZ6323494.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7981..9150
                     /locus_tag="J8I47_002066"
     CDS             7981..9150
                     /locus_tag="J8I47_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ6323495.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9325..10704
                     /locus_tag="J8I47_002067"
     CDS             9325..10704
                     /locus_tag="J8I47_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323496.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3585 a         1882 c         2413 g         2903 t
ORIGIN      
        1 aatagttcgg gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc
       61 gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct
      121 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
      181 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
      241 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
      301 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
      361 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
      421 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
      481 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
      541 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
      601 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
      661 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
      721 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
      781 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
      841 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
      901 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
      961 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
     1021 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
     1081 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     1141 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     1201 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     1261 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     1321 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     1381 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     1441 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     1501 tagccgcttc tttatgcaca aaagaaaaat atgatacaga cggattgatc gccgtaaaaa
     1561 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     1621 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     1681 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     1741 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     1801 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     1861 tatttttagg aacgattcat acagttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     1921 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     1981 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     2041 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     2101 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     2161 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     2221 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     2281 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     2341 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     2401 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     2461 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     2521 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     2581 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     2641 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     2701 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     2761 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     2821 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     2881 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     2941 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     3001 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     3061 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     3121 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     3181 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     3241 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     3301 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     3361 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     3421 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     3481 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     3541 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     3601 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     3661 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     3721 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     3781 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     3841 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     3901 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     3961 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     4021 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     4081 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     4141 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     4201 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     4261 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     4321 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     4381 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     4441 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     4501 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     4561 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     4621 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     4681 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     4741 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     4801 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     4861 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     4921 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     4981 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     5041 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     5101 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
     5161 atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
     5221 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
     5281 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
     5341 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
     5401 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
     5461 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
     5521 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
     5581 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
     5641 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
     5701 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
     5761 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
     5821 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
     5881 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
     5941 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
     6001 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
     6061 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
     6121 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
     6181 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
     6241 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
     6301 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
     6361 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
     6421 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
     6481 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
     6541 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
     6601 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
     6661 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
     6721 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
     6781 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
     6841 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
     6901 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcggggccg
     6961 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
     7021 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
     7081 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
     7141 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
     7201 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
     7261 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
     7321 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
     7381 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
     7441 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
     7501 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgcaatgtt ttagctggag
     7561 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
     7621 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
     7681 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
     7741 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
     7801 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
     7861 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
     7921 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
     7981 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
     8041 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
     8101 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
     8161 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
     8221 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
     8281 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
     8341 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
     8401 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
     8461 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
     8521 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
     8581 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
     8641 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
     8701 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
     8761 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
     8821 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
     8881 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
     8941 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
     9001 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
     9061 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
     9121 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
     9181 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
     9241 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
     9301 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
     9361 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
     9421 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
     9481 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
     9541 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
     9601 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
     9661 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
     9721 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
     9781 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
     9841 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
     9901 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
     9961 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
    10021 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
    10081 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    10141 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    10201 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    10261 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    10321 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    10381 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    10441 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    10501 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    10561 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    10621 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    10681 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    10741 tgccttgcat caagtaataa gaacagccaa gtaaaaacag ctt
//
LOCUS       DADOZM010000098        10741 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.101, whole genome shotgun sequence.
ACCESSION   DADOZM010000098 DADOZM010000000
VERSION     DADOZM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.101"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            291..1991
                     /locus_tag="J8I47_002068"
     CDS             291..1991
                     /locus_tag="J8I47_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323497.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2093..2926)
                     /locus_tag="J8I47_002069"
     CDS             complement(2093..2926)
                     /locus_tag="J8I47_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAZ6323498.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3230..5395)
                     /locus_tag="J8I47_002070"
     CDS             complement(3230..5395)
                     /locus_tag="J8I47_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323499.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5655..7949
                     /locus_tag="J8I47_002071"
     CDS             5655..7949
                     /locus_tag="J8I47_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAZ6323500.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            7942..8607
                     /gene="pgmB"
                     /locus_tag="J8I47_002072"
     CDS             7942..8607
                     /gene="pgmB"
                     /locus_tag="J8I47_002072"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAZ6323501.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            8600..9607
                     /locus_tag="J8I47_002073"
     CDS             8600..9607
                     /locus_tag="J8I47_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ6323502.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9629..10648
                     /locus_tag="J8I47_002074"
     CDS             9629..10648
                     /locus_tag="J8I47_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323503.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3248 a         2283 c         1948 g         3262 t
ORIGIN      
        1 tgcgttatgc tttgagtaat aagagcgcta gtcagaaaac agcttctcat attttctagg
       61 ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa
      121 aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa
      181 acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt aaaattcata
      241 aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat atgatgaatt
      301 tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt ttagcaattt
      361 ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta attttgggtg
      421 ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt aaatatgggg
      481 atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc gtgaatgccg
      541 tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca ggtagctggt
      601 ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt tctatgctga
      661 ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc gttttattca
      721 tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc tgggggactt
      781 gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa aagttccctg
      841 ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg tcttcgatca
      901 tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag actgaaaaag
      961 gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg acagcaaata
     1021 tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc tttgttaaca
     1081 gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat taccctaagg
     1141 cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa caaaagaaca
     1201 ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt tctactagct
     1261 tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg atccagtttc
     1321 catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc ctttcactga
     1381 accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt cttatttttt
     1441 cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat gaaacatttg
     1501 tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca
     1561 aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat ctgccaatct
     1621 ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta gaaaacgaaa
     1681 caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa aacactgggg
     1741 acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag caaaatggaa
     1801 aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag cagaaaaaag
     1861 gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc attctttctc
     1921 ttactttgat tggctggttt accttgctat gtttatttat ttatcataga tacaagcttt
     1981 tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa acggtataat
     2041 cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt
     2101 tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat
     2161 caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac
     2221 gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc
     2281 ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat
     2341 aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag
     2401 tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg
     2461 tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc
     2521 caatcaagat cttgcgtgtc tgataatctt ctttcttttg agattgggag acggtaagga
     2581 cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac
     2641 cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa
     2701 ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc tgacagaact
     2761 tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag
     2821 caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg
     2881 gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg
     2941 tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata
     3001 gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg
     3061 gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc
     3121 aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
     3181 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa
     3241 tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta
     3301 ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat
     3361 caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa
     3421 tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga
     3481 taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc
     3541 cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca
     3601 ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg
     3661 cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg
     3721 tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg
     3781 atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca
     3841 atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta
     3901 cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca
     3961 gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa
     4021 cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt
     4081 ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca
     4141 cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca
     4201 attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag
     4261 ccccttggat cactgaataa atgacatata atggcacagc agcaaacata aacatgaact
     4321 ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt
     4381 tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca
     4441 agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca
     4501 acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc
     4561 aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa
     4621 tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc
     4681 caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc
     4741 tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc
     4801 acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa
     4861 agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat
     4921 tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag
     4981 ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag
     5041 tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag
     5101 tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt
     5161 ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc
     5221 aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca
     5281 agggaattga tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa
     5341 ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt
     5401 ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt
     5461 aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt
     5521 tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt
     5581 tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca
     5641 ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca
     5701 gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa
     5761 acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag
     5821 gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg
     5881 gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt
     5941 cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat
     6001 tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag
     6061 tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt
     6121 taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa
     6181 atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa
     6241 ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac
     6301 aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact
     6361 atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga
     6421 catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc
     6481 aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag
     6541 aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag
     6601 acgaagaagc acaacaaggt attcgattca atttgttcca actattctct acttactacg
     6661 gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag
     6721 ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg
     6781 aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg
     6841 ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat
     6901 gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg
     6961 caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg
     7021 aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa
     7081 ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca
     7141 attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa
     7201 cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata
     7261 tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca
     7321 cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga
     7381 atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac
     7441 tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg
     7501 atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt
     7561 tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct
     7621 tggatttgga taattacaat aacgacacag aagacggctt gcacatcact tccatgactg
     7681 gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga
     7741 gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg
     7801 gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt
     7861 ttcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa
     7921 cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt
     7981 accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt
     8041 acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct
     8101 ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta
     8161 gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc
     8221 ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt
     8281 gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt
     8341 gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc
     8401 cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa
     8461 gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa
     8521 gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg
     8581 aaagaaaagt gtcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac
     8641 tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg
     8701 tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt
     8761 gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg
     8821 acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg
     8881 cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga
     8941 agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg aattttctac
     9001 ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat
     9061 gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt
     9121 caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga
     9181 tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa
     9241 actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga
     9301 tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt
     9361 attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt
     9421 attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa
     9481 ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg
     9541 atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc
     9601 acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa
     9661 agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga
     9721 gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac
     9781 tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga
     9841 ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga
     9901 agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt
     9961 agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc
    10021 agatagtcaa gatcctgtca ttgattattt ataccaaaac cacatccctt tcactttgat
    10081 cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct
    10141 aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac
    10201 aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat
    10261 gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc
    10321 ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt
    10381 cgcccttcgc acgatgcagt tggcgcaaat gtacggctat gtcattccag agacactctc
    10441 cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat
    10501 cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca
    10561 tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac
    10621 agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc tgaccattta
    10681 tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg tttatctgta
    10741 a
//
LOCUS       DADOZM010000099        10640 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.102, whole genome shotgun sequence.
ACCESSION   DADOZM010000099 DADOZM010000000
VERSION     DADOZM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.102"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(307..1440)
                     /locus_tag="J8I47_002075"
     CDS             complement(307..1440)
                     /locus_tag="J8I47_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6323504.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="J8I47_002076"
     CDS             complement(1431..2099)
                     /locus_tag="J8I47_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323505.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="J8I47_002077"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="J8I47_002077"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ6323506.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="J8I47_002078"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="J8I47_002078"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAZ6323507.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="J8I47_002079"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="J8I47_002079"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAZ6323508.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="J8I47_002080"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="J8I47_002080"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAZ6323509.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="J8I47_002081"
     CDS             complement(5475..6248)
                     /locus_tag="J8I47_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6323510.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="J8I47_002082"
     CDS             6413..6892
                     /locus_tag="J8I47_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323511.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="J8I47_002083"
     CDS             6949..7251
                     /locus_tag="J8I47_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAZ6323512.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="J8I47_002084"
     CDS             7273..8730
                     /locus_tag="J8I47_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAZ6323513.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9323..10075)
                     /locus_tag="J8I47_002085"
     CDS             complement(9323..10075)
                     /locus_tag="J8I47_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6323514.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10526)
                     /locus_tag="J8I47_002086"
                     /pseudo
     CDS             complement(10290..10526)
                     /locus_tag="J8I47_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10536..>10640)
                     /locus_tag="J8I47_002087"
     CDS             complement(10536..>10640)
                     /locus_tag="J8I47_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6323515.1"
                     /translation="ASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         3035 a         2302 c         1807 g         3496 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc
//
LOCUS       DADOZM010000100        10590 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.103, whole genome shotgun sequence.
ACCESSION   DADOZM010000100 DADOZM010000000
VERSION     DADOZM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.103"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(777..2408)
                     /locus_tag="J8I47_002088"
     CDS             complement(777..2408)
                     /locus_tag="J8I47_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAZ6323516.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="J8I47_002089"
     CDS             complement(2422..4095)
                     /locus_tag="J8I47_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323517.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="J8I47_002090"
     CDS             complement(4108..4425)
                     /locus_tag="J8I47_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ6323518.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="J8I47_002091"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="J8I47_002091"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAZ6323519.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="J8I47_002092"
     CDS             6087..6872
                     /locus_tag="J8I47_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6323520.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="J8I47_002093"
     CDS             6875..7567
                     /locus_tag="J8I47_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6323521.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="J8I47_002094"
     CDS             complement(7841..8212)
                     /locus_tag="J8I47_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAZ6323522.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8612..8842)
                     /locus_tag="J8I47_002095"
     CDS             complement(8612..8842)
                     /locus_tag="J8I47_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323523.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8936..9274)
                     /locus_tag="J8I47_002096"
     CDS             complement(8936..9274)
                     /locus_tag="J8I47_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323524.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10202..10318)
                     /locus_tag="J8I47_002097"
     CDS             complement(10202..10318)
                     /locus_tag="J8I47_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAZ6323525.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         3306 a         2217 c         1728 g         3339 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaaata aaatactctg gatactcttt
     8521 atactgcatt aaagttagta atattaacag caaagatatc gaaaaaacaa taacataata
     8581 tgtgtcaaaa tttttcataa taagaactcc cttactgttg catactaaca taatccaaaa
     8641 actcttctac ttcagctaaa gacattccac tttctttagt ccaaaaaaca gttttttcta
     8701 tcgatccttc tctgtaaact gtaaatgtcg gagtcgagtt aattgagtag cgactcttga
     8761 attctttcca ctcattttca tcttctctaa cttttgctac attcacgtaa taaatatttt
     8821 cttttccctt ttctaccagc atatcgaata gcttcctgct tttgatgatc gatttcttgt
     8881 gctacatcta gtaaattttc atcaatttct atatcataaa gtatttcact agtgtctaca
     8941 tccacaatat atacttttta tcagtagcaa tttttgagta atccgttaaa atgatctcag
     9001 tctcgtcact ttcatcagta taaatatacg ttaacaatga actcaccttt tcaatcggtg
     9061 ttagctctaa gcctactgta ttttttacta ttgagtcatt atttaccacc ataacttcgt
     9121 actgtatata ccatcctatt tggtttccat caatatcagt aattggctca ctagctaatg
     9181 gagtctccgg aacaatattt tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt
     9241 tatcatgttc ctcacttgaa actggttgag tcatagaaaa tgttacttgg ctaaacagag
     9301 ggaaaataaa cactacaatc aacaaagtat aaaaaaaagc ccttcttttt cattttttat
     9361 cttccaatca caaattgaaa aacttattcc tgcaactttg agtctagtat cacaatcgac
     9421 tgactacaac tattcattta cattctactt taagttcctc cttaaccatt aattttatta
     9481 aagatacttg taatagtgat tctcacaaaa tttcatgaaa aatactttat tactgtatct
     9541 aaaattccaa tacacaactt atcgactatt atctgaaaac caaaacttat tattccttga
     9601 tgttattcta gcaatagtaa ctgctttttt atttttttgc atattttttc ggcgttactt
     9661 tttaaagtta actttttcga caaatagaaa gagatacgaa tcactgattt ttttgaagaa
     9721 aagaaatgga gtgagctgaa cgttagagat attgggagct ttcgtaactg cttattaaac
     9781 gataatggtt atttgcaacc gtattgctcg cttctacatg aaatatttca tgaaatattt
     9841 cgcaaaacct ttgattatgt agttacgttt ccattcttga ctaaaaatgt ttcaaaaacg
     9901 aacaaaggtg ttaaaaggta aagtgatcaa tactactggt caaaataaga ggttgaaagg
     9961 gaattgaaaa ataactacag ataatacttt tataaacgta cgtgctttgt ggttctgtag
    10021 ttaactatat aactggaatt agggaatggt atcaatttga aaagaaagat tctaccaatt
    10081 tatcatatgt taaaaatgga aaatcaacag cagttctttc ggaaacctta cacaaaaacc
    10141 gattattgaa aatagccgaa aaacgttttt cggctatttt tttaattaag tttccttctt
    10201 cttacctttt cccactattc cgtttcacat ctttgatgat cttcagcgca cgttttcgtg
    10261 ttttgatatt tgggcttttc aagcggcggt atgcacttga gatatctgtt ttttccatat
    10321 ggacgaccca ctttcttctt tgattgtagt catgctaaaa aacacaaatc gaaacaatta
    10381 cgatttgtgt tttaaatggt atcaaaagtt aaaatgaaag tcaacttgtt aaaacagtcg
    10441 atcagactag ctgattgact cctatctctg gaaataaatt ttcattatta cgttttagct
    10501 acctaaaaaa ggccctcttg tccgttaact ttaatatcta ttgttatagc tagtttacct
    10561 attgaacgta ctgtgtgttc gatagaacac
//
LOCUS       DADOZM010000101        10520 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.104, whole genome shotgun sequence.
ACCESSION   DADOZM010000101 DADOZM010000000
VERSION     DADOZM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.104"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            23..646
                     /locus_tag="J8I47_002098"
     CDS             23..646
                     /locus_tag="J8I47_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323526.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="J8I47_002099"
     CDS             1096..2145
                     /locus_tag="J8I47_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323527.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="J8I47_002100"
     CDS             2142..3023
                     /locus_tag="J8I47_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323528.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="J8I47_002101"
     CDS             3026..3796
                     /locus_tag="J8I47_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ6323529.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="J8I47_002102"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="J8I47_002102"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAZ6323530.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="J8I47_002103"
     CDS             5107..6270
                     /locus_tag="J8I47_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAZ6323531.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="J8I47_002104"
     CDS             6387..6797
                     /locus_tag="J8I47_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ6323532.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="J8I47_002105"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="J8I47_002105"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ6323533.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="J8I47_002106"
     CDS             9337..9654
                     /locus_tag="J8I47_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323534.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="J8I47_002107"
     CDS             9814..10050
                     /locus_tag="J8I47_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ6323535.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="J8I47_002108"
     CDS             10047..10445
                     /locus_tag="J8I47_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAZ6323536.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3662 a         1768 c         2229 g         2861 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca
//
LOCUS       DADOZM010000102        10095 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.105, whole genome shotgun sequence.
ACCESSION   DADOZM010000102 DADOZM010000000
VERSION     DADOZM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.105"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="J8I47_002109"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="J8I47_002109"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAZ6323537.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="J8I47_002110"
     CDS             complement(1172..1867)
                     /locus_tag="J8I47_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAZ6323538.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="J8I47_002111"
     CDS             complement(1880..2596)
                     /locus_tag="J8I47_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAZ6323539.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="J8I47_002112"
     CDS             2926..3534
                     /locus_tag="J8I47_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAZ6323540.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="J8I47_002113"
     CDS             3859..4182
                     /locus_tag="J8I47_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAZ6323541.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="J8I47_002114"
     CDS             4364..5734
                     /locus_tag="J8I47_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAZ6323542.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="J8I47_002115"
     CDS             complement(5864..7165)
                     /locus_tag="J8I47_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAZ6323543.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="J8I47_002116"
     CDS             complement(7519..9510)
                     /locus_tag="J8I47_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAZ6323544.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="J8I47_002117"
     CDS             complement(9654..9920)
                     /locus_tag="J8I47_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ6323545.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2958 a         2096 c         1833 g         3208 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcg
//
LOCUS       DADOZM010000103        10090 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.106, whole genome shotgun sequence.
ACCESSION   DADOZM010000103 DADOZM010000000
VERSION     DADOZM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.106"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            162..392
                     /locus_tag="J8I47_002118"
     CDS             162..392
                     /locus_tag="J8I47_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAZ6323546.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="J8I47_002119"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="J8I47_002119"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAZ6323547.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="J8I47_002120"
     CDS             1803..1979
                     /locus_tag="J8I47_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAZ6323548.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="J8I47_002121"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="J8I47_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAZ6323549.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="J8I47_002122"
     CDS             2474..2941
                     /locus_tag="J8I47_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323550.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="J8I47_002123"
     CDS             3026..3910
                     /locus_tag="J8I47_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6323551.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="J8I47_002124"
     CDS             4027..4260
                     /locus_tag="J8I47_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAZ6323552.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="J8I47_002125"
     tRNA            4348..4428
                     /locus_tag="J8I47_002125"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="J8I47_002126"
     tRNA            4435..4506
                     /locus_tag="J8I47_002126"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="J8I47_002127"
     CDS             5021..5728
                     /locus_tag="J8I47_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323553.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="J8I47_002128"
     CDS             complement(5788..6432)
                     /locus_tag="J8I47_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAZ6323554.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="J8I47_002129"
     CDS             6559..6846
                     /locus_tag="J8I47_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323555.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="J8I47_002130"
     CDS             6954..7274
                     /locus_tag="J8I47_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323556.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="J8I47_002131"
     CDS             7355..8668
                     /locus_tag="J8I47_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ6323557.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="J8I47_002132"
     CDS             8665..9384
                     /locus_tag="J8I47_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAZ6323558.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="J8I47_002133"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="J8I47_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAZ6323559.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1669 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa
//
LOCUS       DADOZM010000104        10086 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.107, whole genome shotgun sequence.
ACCESSION   DADOZM010000104 DADOZM010000000
VERSION     DADOZM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.107"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(247..759)
                     /locus_tag="J8I47_002134"
     CDS             complement(247..759)
                     /locus_tag="J8I47_002134"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAZ6323560.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="J8I47_002135"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="J8I47_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAZ6323561.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="J8I47_002136"
     CDS             complement(3212..3646)
                     /locus_tag="J8I47_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAZ6323562.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="J8I47_002137"
     CDS             complement(3656..4444)
                     /locus_tag="J8I47_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6323563.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="J8I47_002138"
     CDS             complement(4441..5382)
                     /locus_tag="J8I47_002138"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAZ6323564.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="J8I47_002139"
     CDS             complement(5559..6146)
                     /locus_tag="J8I47_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAZ6323565.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="J8I47_002140"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="J8I47_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAZ6323566.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="J8I47_002141"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="J8I47_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAZ6323567.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="J8I47_002142"
     CDS             complement(9063..9998)
                     /locus_tag="J8I47_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6323568.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DADOZM010000105         9942 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.108, whole genome shotgun sequence.
ACCESSION   DADOZM010000105 DADOZM010000000
VERSION     DADOZM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.108"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(405..629)
                     /locus_tag="J8I47_002143"
     CDS             complement(405..629)
                     /locus_tag="J8I47_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323569.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            829..3597
                     /locus_tag="J8I47_002144"
     CDS             829..3597
                     /locus_tag="J8I47_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6323570.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3678..4097
                     /locus_tag="J8I47_002145"
     CDS             3678..4097
                     /locus_tag="J8I47_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAZ6323571.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4109..4579
                     /locus_tag="J8I47_002146"
     CDS             4109..4579
                     /locus_tag="J8I47_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323572.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4596..5375
                     /locus_tag="J8I47_002147"
     CDS             4596..5375
                     /locus_tag="J8I47_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323573.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5362..6183
                     /locus_tag="J8I47_002148"
     CDS             5362..6183
                     /locus_tag="J8I47_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6323574.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6200..7249
                     /locus_tag="J8I47_002149"
     CDS             6200..7249
                     /locus_tag="J8I47_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6323575.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7262..8266
                     /locus_tag="J8I47_002150"
     CDS             7262..8266
                     /locus_tag="J8I47_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6323576.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8899..9918
                     /locus_tag="J8I47_002151"
     CDS             8899..9918
                     /locus_tag="J8I47_002151"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAZ6323577.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3336 a         1645 c         2080 g         2881 t
ORIGIN      
        1 ggagtatgaa aatgagaaaa atatttattc gtttattgtg ggtactatct tagaggaata
       61 aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga
      121 caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa
      181 aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag
      241 agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag
      301 catcataact cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat
      361 ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag
      421 gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat
      481 tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa
      541 aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt
      601 ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata
      661 tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata
      721 agggataagt aggctgctgt ctataataag atatattttt caattcttta ttaaagattt
      781 tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag
      841 gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag
      901 gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat
      961 tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt
     1021 tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt
     1081 tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct
     1141 tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt
     1201 tactatccag atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt
     1261 tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca
     1321 ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat
     1381 ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt
     1441 gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt
     1501 caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat
     1561 ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat
     1621 ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag
     1681 cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa
     1741 atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta
     1801 tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct
     1861 gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt
     1921 gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc
     1981 ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag
     2041 caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta
     2101 aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa
     2161 cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact
     2221 gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag
     2281 cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta
     2341 gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa
     2401 ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc
     2461 tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct
     2521 cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat
     2581 ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc
     2641 cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga
     2701 tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg
     2761 aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca
     2821 ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat
     2881 ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg
     2941 gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc
     3001 ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac
     3061 gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca
     3121 ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact
     3181 atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa
     3241 aaagtactgg attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat
     3301 tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg
     3361 ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc
     3421 tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct
     3481 gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat
     3541 atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca
     3601 taagcaagtt gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga
     3661 aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt
     3721 cagggatatt agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg
     3781 attgctatac tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt
     3841 acaaagataa agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat
     3901 tcttacaata tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat
     3961 tgattgagct agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg
     4021 tgctaactga ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag
     4081 aagaagacga attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt
     4141 attacacgga caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat
     4201 tgctaatgat gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc
     4261 acaaggagtc aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg
     4321 ggtgacagat aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct
     4381 ggcagaagcg tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg
     4441 tactcgcagc atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg
     4501 attaattgac aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga
     4561 tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag
     4621 gaattgttgc atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc
     4681 cgattgtgac ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta
     4741 tgggcgcaac tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac
     4801 ctgatgttgt gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta
     4861 cagaaaccgc gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata
     4921 tttacttagg gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag
     4981 gaaattataa aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc
     5041 ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa
     5101 atatgattcc tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc
     5161 ttggttttgc gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct
     5221 taggttttgc tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg
     5281 cgattttagc agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa
     5341 ctgcagggga ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa
     5401 aaagagctaa atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa
     5461 cgtcagatga atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag
     5521 tcgaaagaag aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca
     5581 catatcgtta cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat
     5641 cctgaatttg atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca
     5701 gggttaggag attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc
     5761 tctttagcca tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt
     5821 ccgcacattc tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt
     5881 ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt
     5941 ggactgatgg tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa
     6001 atcggttcag gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat
     6061 atcttatgtc ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca
     6121 ttaacgatct tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt
     6181 taaaaaagga gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt
     6241 actaagtacg atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt
     6301 gaatttactg atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc
     6361 attggaatct tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta
     6421 tggaaaactt tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc
     6481 ttctactgtt gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac
     6541 aaatgacgtg caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg
     6601 ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact
     6661 cttagggata ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta
     6721 catgcaacag ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga
     6781 attttttgat ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata
     6841 tggtccaaac tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt
     6901 tccgtcacaa ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc
     6961 ttcgcatttg ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt
     7021 gcaaagatat atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag
     7081 aaacgagaaa acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct
     7141 tgtcgttccg tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa
     7201 caaaaagatt tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat
     7261 catgctgaaa ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg
     7321 tccgcaagta gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta
     7381 tctaggaatc ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa
     7441 aagcaacttg cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag
     7501 attaacaaaa gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt
     7561 gaaagcagta gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc
     7621 gaacagtaag ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga
     7681 acaaatcaaa ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc
     7741 ttattcagaa tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga
     7801 gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca
     7861 ttattttatt ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg
     7921 ggaagaacaa agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac
     7981 gttagagatc gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag
     8041 agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat
     8101 agacacaaaa gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc
     8161 tttcttattg atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg
     8221 ccatccatta gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa
     8281 aaagaatata aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt
     8341 ggcatcaatt acgttataaa acacattttt tgcattatct acagttcaac ttataatctg
     8401 tttaatggga catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt
     8461 gcatggatcc gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg
     8521 tatccagaat gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt
     8581 ccgttgcagg acctgacaga tttagaaatg ttcaatctgt ctcttatgta ttttaaatca
     8641 atactcacta ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa
     8701 aactgcttcc tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat
     8761 tgtaacatat cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa
     8821 aacaatggaa attagtccca actttaagaa atgtatacta taatggaaat tatcaattat
     8881 ttatttggag gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc
     8941 actgctttag cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta
     9001 cctgaacaga ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg
     9061 acgatcccag aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat
     9121 gctattttat ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt
     9181 aaaatgaata ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat
     9241 aaacgaatct cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt
     9301 gtattatctg gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca
     9361 gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat
     9421 ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat
     9481 atcattgcaa ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca
     9541 gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat
     9601 cctctgacat ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt
     9661 cattctcgca attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt
     9721 ctagaaaaca tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta
     9781 gctcaacagc taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat
     9841 ggagaagata tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat
     9901 gaattttctc ttcgttaaat agatgatcaa tagttaatcg tc
//
LOCUS       DADOZM010000106         9820 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.109, whole genome shotgun sequence.
ACCESSION   DADOZM010000106 DADOZM010000000
VERSION     DADOZM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.109"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..195)
                     /locus_tag="J8I47_002152"
     CDS             complement(<1..195)
                     /locus_tag="J8I47_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323578.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="J8I47_002153"
     CDS             complement(211..825)
                     /locus_tag="J8I47_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAZ6323579.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="J8I47_002154"
     CDS             complement(972..1655)
                     /locus_tag="J8I47_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAZ6323580.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="J8I47_002155"
     CDS             complement(1764..2222)
                     /locus_tag="J8I47_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAZ6323581.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="J8I47_002156"
     CDS             complement(2306..4069)
                     /locus_tag="J8I47_002156"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAZ6323582.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="J8I47_002157"
     CDS             complement(4290..5750)
                     /locus_tag="J8I47_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323583.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="J8I47_002158"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="J8I47_002158"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAZ6323584.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="J8I47_002159"
     CDS             complement(6907..8334)
                     /locus_tag="J8I47_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6323585.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="J8I47_002160"
     CDS             complement(8365..9579)
                     /locus_tag="J8I47_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAZ6323586.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DADOZM010000107         9416 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.110, whole genome shotgun sequence.
ACCESSION   DADOZM010000107 DADOZM010000000
VERSION     DADOZM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.110"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            100..294
                     /locus_tag="J8I47_002161"
     CDS             100..294
                     /locus_tag="J8I47_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323587.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            563..862
                     /locus_tag="J8I47_002162"
     CDS             563..862
                     /locus_tag="J8I47_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323588.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(959..1393)
                     /locus_tag="J8I47_002163"
     CDS             complement(959..1393)
                     /locus_tag="J8I47_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323589.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1625..2335)
                     /locus_tag="J8I47_002164"
     CDS             complement(1625..2335)
                     /locus_tag="J8I47_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAZ6323590.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2325..3185)
                     /locus_tag="J8I47_002165"
     CDS             complement(2325..3185)
                     /locus_tag="J8I47_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAZ6323591.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3190..3828)
                     /locus_tag="J8I47_002166"
     CDS             complement(3190..3828)
                     /locus_tag="J8I47_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAZ6323592.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3843..4142)
                     /locus_tag="J8I47_002167"
     CDS             complement(3843..4142)
                     /locus_tag="J8I47_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323593.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4185..5669)
                     /locus_tag="J8I47_002168"
     CDS             complement(4185..5669)
                     /locus_tag="J8I47_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAZ6323594.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5689..6150)
                     /locus_tag="J8I47_002169"
     CDS             complement(5689..6150)
                     /locus_tag="J8I47_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ6323595.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="J8I47_002170"
     CDS             complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="J8I47_002170"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAZ6323596.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7453..8223
                     /locus_tag="J8I47_002171"
     CDS             7453..8223
                     /locus_tag="J8I47_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ6323597.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8496..9323)
                     /locus_tag="J8I47_002172"
     CDS             complement(8496..9323)
                     /locus_tag="J8I47_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6323598.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2929 a         1931 c         1479 g         3077 t
ORIGIN      
        1 ttgattcttt ttatttatca taaagatata gatacataat cattttcact tacatatatt
       61 tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt agaagtaaaa
      121 acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata tcctttactt
      181 ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat aaagaacgag
      241 ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa gtaaatccaa
      301 tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga ttatagaatt
      361 agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat accatatgat
      421 tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga tgaatcaaat
      481 atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt tataatgtat
      541 cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg aactacttaa
      601 gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca aagtgacaga
      661 gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat
      721 atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa
      781 aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat ttgataaaga
      841 ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat actaagtcta
      901 tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt attatatgtt
      961 atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa gaaataccac
     1021 ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt ttctcagtat
     1081 catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc aaagcttcat
     1141 tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg atatcttgaa
     1201 taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc ttcccttccc
     1261 cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt gcagtgtctt
     1321 ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg atcataaggg
     1381 aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata atacagttct
     1441 ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat tccttaaaat
     1501 tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga taaattttct
     1561 tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc ttaccctagc
     1621 atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt ttatctagta
     1681 aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg gccatcagac
     1741 aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc ccccatgtga
     1801 acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat tttctttcct
     1861 taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt acttcttctt
     1921 tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga gtcgtgccat
     1981 aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag
     2041 taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg agatctgaag
     2101 atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc accatttgat
     2161 ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact cctagctcac
     2221 ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc ggcaatgcca
     2281 gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa gacatcatag
     2341 ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc tcttcgaaag
     2401 tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc tagacgtttc
     2461 aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac atcacgaaac
     2521 tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc agtgatataa
     2581 tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga caaattgcct
     2641 aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt
     2701 gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat gccatattta
     2761 gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga attcattgat
     2821 ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct tagattatgc
     2881 gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc tggatcagca
     2941 gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg tacaccggag
     3001 ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa tcgtgctaac
     3061 cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc ttttacaagg
     3121 gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat cccaatttga
     3181 gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg cggccttcat
     3241 cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat cgatcccttc
     3301 aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa agcctagttc
     3361 aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag ataacagcgc
     3421 atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat ccatggcttg
     3481 ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt tttgtgctgc
     3541 ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc ctgcttttgc
     3601 aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga ctagtttttt
     3661 atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta agcaaagaat
     3721 tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc ctaatgcatg
     3781 ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct tagttccttc
     3841 ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat ttctttgact
     3901 gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg agtaaacata
     3961 ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact ttttccctct
     4021 gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat tgctttttcc
     4081 acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac taaaattctc
     4141 atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac tctctgcttt
     4201 tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt tttccgatat
     4261 tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc ataacctagg
     4321 actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat cccgatataa
     4381 ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc tacttgtatg
     4441 attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt attggcataa
     4501 actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac gatcgttggt
     4561 gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc aaacaagaac
     4621 ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc aattcctgga
     4681 acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag ttcatcgacg
     4741 aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa agaagtcgtc
     4801 ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat caaataatct
     4861 ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga agtagcaacc
     4921 atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat ttttttgtct
     4981 tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc taattttgca
     5041 aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc ttctgtcaat
     5101 tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc taaaatcagt
     5161 cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag taaaatccaa
     5221 aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga acgcagtttc
     5281 gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag taataaaatc
     5341 atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac agcattttgt
     5401 ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag tccaaccaag
     5461 attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa gtaaccgacc
     5521 gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa aatatagcct
     5581 aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt tgcaaaatac
     5641 gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt aattatattc
     5701 tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct caaccaatcc
     5761 atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt ctggatcttt
     5821 tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt cactttcaaa
     5881 acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac catcactttt
     5941 ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac cgtatttttc
     6001 tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat cgatcaatac
     6061 aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg ggtaatgaat
     6121 caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta taaaaacgac
     6181 ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata aaaattaaac
     6241 tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca gatataaggt
     6301 ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat ctcttttgga
     6361 tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt tgcattcaat
     6421 gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc tcgtgggttt
     6481 tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt ggcaaataaa
     6541 ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc gaataaatag
     6601 tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc ttctggatct
     6661 tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt gatgtctttg
     6721 attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat tccccatttt
     6781 tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca attttgatga
     6841 actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac gattaaagcg
     6901 tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa attaggctgc
     6961 atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat gccattccca
     7021 tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc ttcatgactt
     7081 ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg atccactact
     7141 tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc tactaaccac
     7201 ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc atatcgttgt
     7261 tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt ttatatgacc
     7321 gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat aataaagaca
     7381 gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga tgtccctcag
     7441 gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg
     7501 gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc ccttgatgta
     7561 tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga gatcatacgc
     7621 aattacggtt attgttctta taattacaaa aaccagactg attttgatca atctggtcct
     7681 gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga ttatgatacg
     7741 ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca atctaaccat
     7801 ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga taattgtagt
     7861 tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg cgacatcgca
     7921 ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag cggagtcgat
     7981 gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga aatgatggtc
     8041 aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg actgacatca
     8101 aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa attttgttct
     8161 gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt agattcattt
     8221 taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa caaatttatc
     8281 gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg tatccatcca
     8341 atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc taaataggaa
     8401 tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt tcgaataaac
     8461 ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc
     8521 tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat atatctttct
     8581 aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag caagtattcc
     8641 aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca taattggatt
     8701 gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata tacaatgcag
     8761 accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc cacataaact
     8821 ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc agctgcttgt
     8881 aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata aaataatgct
     8941 gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa aatttgattt
     9001 aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt ataagattct
     9061 tggtattttg atacagaatt caatgattga atctcattta tttttttcct gattttgtct
     9121 tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca aagcgcactt
     9181 aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt tgcttgttca
     9241 caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta ttcttttagt
     9301 aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta tttctccttt
     9361 atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga ataaaa
//
LOCUS       DADOZM010000108         9333 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.111, whole genome shotgun sequence.
ACCESSION   DADOZM010000108 DADOZM010000000
VERSION     DADOZM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.111"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1412)
                     /locus_tag="J8I47_002173"
     CDS             complement(<1..1412)
                     /locus_tag="J8I47_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ6323599.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFN"
     gene            complement(1414..2082)
                     /locus_tag="J8I47_002174"
     CDS             complement(1414..2082)
                     /locus_tag="J8I47_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAZ6323600.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2212..2796)
                     /locus_tag="J8I47_002175"
     CDS             complement(2212..2796)
                     /locus_tag="J8I47_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323601.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3127..3360)
                     /locus_tag="J8I47_002176"
     CDS             complement(3127..3360)
                     /locus_tag="J8I47_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAZ6323602.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3375..4325)
                     /locus_tag="J8I47_002177"
     CDS             complement(3375..4325)
                     /locus_tag="J8I47_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323603.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4322..5164)
                     /locus_tag="J8I47_002178"
     CDS             complement(4322..5164)
                     /locus_tag="J8I47_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ6323604.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5166..5885)
                     /locus_tag="J8I47_002179"
     CDS             complement(5166..5885)
                     /locus_tag="J8I47_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323605.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6577..6735
                     /locus_tag="J8I47_002180"
     CDS             6577..6735
                     /locus_tag="J8I47_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323606.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6807..8033
                     /locus_tag="J8I47_002181"
     CDS             6807..8033
                     /locus_tag="J8I47_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323607.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8121..8513)
                     /gene="rpsI"
                     /locus_tag="J8I47_002182"
     CDS             complement(8121..8513)
                     /gene="rpsI"
                     /locus_tag="J8I47_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAZ6323608.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8529..8972)
                     /gene="rplM"
                     /locus_tag="J8I47_002183"
     CDS             complement(8529..8972)
                     /gene="rplM"
                     /locus_tag="J8I47_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAZ6323609.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         3175 a         1629 c         1306 g         3223 t
ORIGIN      
        1 tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat atcttttttt
       61 attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa ttccggatca
      121 tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc agcaatcctt
      181 tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa attagttttt
      241 gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa atcaaacggt
      301 aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg ctgatcttta
      361 ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa aaattggtaa
      421 tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc gattatcttt
      481 cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt ccccacaccg
      541 tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat gtcttttaga
      601 atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt ttcgatatag
      661 ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt
      721 tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca
      781 attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt
      841 tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac
      901 ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat agaaattttc
      961 tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat
     1021 aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc
     1081 atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga
     1141 cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt
     1201 tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt
     1261 aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct
     1321 ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa
     1381 gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat
     1441 agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact
     1501 tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat
     1561 catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa
     1621 aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg
     1681 ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg
     1741 tattttttct aaagaggata ataattcatt gctagtatag acactaacca acaaccatga
     1801 gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt
     1861 aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat
     1921 caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat
     1981 aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa
     2041 aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa
     2101 taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga
     2161 tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag
     2221 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
     2281 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
     2341 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
     2401 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
     2461 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
     2521 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
     2581 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
     2641 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
     2701 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
     2761 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
     2821 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
     2881 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
     2941 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
     3001 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
     3061 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
     3121 agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa
     3181 ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga
     3241 tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt
     3301 tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat
     3361 acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc
     3421 atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt
     3481 gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc atccacactg
     3541 ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt
     3601 tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta
     3661 tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc
     3721 ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac
     3781 ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt
     3841 tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc
     3901 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
     3961 tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct
     4021 ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca
     4081 tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa
     4141 tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact
     4201 acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc
     4261 ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc
     4321 atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa
     4381 ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat
     4441 aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca
     4501 tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa
     4561 caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca
     4621 tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa
     4681 aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa
     4741 cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat
     4801 gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag
     4861 aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt tttataaaag
     4921 taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta
     4981 aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag
     5041 acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag
     5101 atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa
     5161 gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt taaattttct
     5221 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
     5281 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
     5341 tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct
     5401 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
     5461 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
     5521 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
     5581 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
     5641 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
     5701 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
     5761 gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
     5821 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
     5881 aacataactt tctccttcta aatgataatg attatcacga tcatatggta tacaaaaatt
     5941 atattttagt caaccctaaa ttttatagtt tagatttttt tctgttgctg aagattagaa
     6001 tgacaaaact atttgaaact acgcgtatag taagtagctt tcaaaatata taaaaaagga
     6061 gcgttctcaa atgagaacat gctcctaaaa ctactttatt ggcgtatgga aaaattatac
     6121 ttgaagtgga tagcgggttg aataagttga taagcatcaa aacatttata aaaattattc
     6181 aagactatgt ctgttccata tacaaatatg aaaatcagta aactccattt aacttttttc
     6241 gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa aaaactattt
     6301 ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc gagctagaaa
     6361 ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt tgttctggga
     6421 aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct cgccgtaaac
     6481 tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt cataactact
     6541 attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca aacctgtgca
     6601 tttctaggaa tcgataataa tactctagat aagtggattc gtatatataa tttcccagta
     6661 ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt tatgaataca
     6721 tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga aatatactgc
     6781 cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg aaatataaaa
     6841 ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca gaaaagtatc
     6901 aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg aagaagaaaa
     6961 tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga aaaataacgt
     7021 ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa acaattagat
     7081 caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt cggttgcata
     7141 ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact gagataatat
     7201 cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata aaaggatatg
     7261 ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat cgaactacta
     7321 aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa agaatacaag
     7381 aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca gtaaatgatg
     7441 attttaataa tcatttactt acttttcatg attacacact ttttatgatt accctgtatc
     7501 ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat tttgaaaaac
     7561 aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt acaaaaggtc
     7621 gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt gaatggaaat
     7681 cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat cagtatctct
     7741 ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat tacctaaact
     7801 atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct ccacacaaac
     7861 tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat caaatttcaa
     7921 atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca ccagatatag
     7981 ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat tgatatgtgt
     8041 tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa agtgccagaa
     8101 acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg tgctttctta
     8161 agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc agcacgttta
     8221 agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc atgacggata
     8281 gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc atatgcgcct
     8341 tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc atgtggaatg
     8401 tattcttcaa catctttttt atttacagta attttgccag ttcctggtac tagacgtacg
     8461 cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc caatgaaatt
     8521 ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc tgcatgtgga
     8581 tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa agtgttttta
     8641 ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc acgcaattcg
     8701 ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata aattttgtca
     8761 gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac gaaatcgcca
     8821 gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga tgcaacaact
     8881 gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt acgttctact
     8941 tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt aagttctgat
     9001 gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac catttaacat
     9061 attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa tttatatgaa
     9121 actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa tgaaaaacaa
     9181 aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg gtgttctcct
     9241 gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt tttttaaaat
     9301 tgtttcttgt gactcggagc tgaacggcag gct
//
LOCUS       DADOZM010000109         9238 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.112, whole genome shotgun sequence.
ACCESSION   DADOZM010000109 DADOZM010000000
VERSION     DADOZM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.112"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(310..807)
                     /locus_tag="J8I47_002184"
     CDS             complement(310..807)
                     /locus_tag="J8I47_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ6323610.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="J8I47_002185"
     CDS             complement(847..1656)
                     /locus_tag="J8I47_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6323611.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="J8I47_002186"
     CDS             1990..2847
                     /locus_tag="J8I47_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAZ6323612.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="J8I47_002187"
     CDS             2840..3742
                     /locus_tag="J8I47_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAZ6323613.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="J8I47_002188"
     CDS             3775..4125
                     /locus_tag="J8I47_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ6323614.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="J8I47_002189"
     CDS             4159..5097
                     /locus_tag="J8I47_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ6323615.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="J8I47_002190"
     CDS             5175..6068
                     /locus_tag="J8I47_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6323616.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="J8I47_002191"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="J8I47_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAZ6323617.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="J8I47_002192"
     CDS             6683..6871
                     /locus_tag="J8I47_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAZ6323618.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="J8I47_002193"
     CDS             6884..7786
                     /locus_tag="J8I47_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ6323619.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="J8I47_002194"
     CDS             7818..8060
                     /locus_tag="J8I47_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ6323620.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="J8I47_002195"
     CDS             complement(8316..8687)
                     /locus_tag="J8I47_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323621.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3246 a         1582 c         1779 g         2631 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatgtt
     8821 aaaaataaaa aagaattgtc catcactgtt attcaaatca ttttatcaaa actacatagt
     8881 aaatgttaaa aagcaacatg acataatata tagaaggctt tacagtatat atgggagctt
     8941 actaaaaaag aatggagtaa gagttaacac aatgggccat tagctgaagt tttaagcact
     9001 tcaaggtatg aaacagaatg gcctgtaata ctacatgatt atttagactg ctcaaactgc
     9061 tatcagtctg gtggaatgaa taaagtatta tactaaaagc agatccaact acaacgtaac
     9121 aggaaaccag ttgtatcagg aaaagtgata gtacatggag aagaaaaggc cccctttgta
     9181 tgagtcagta catatcttat ttatagataa aacggtattc aatcgaaata aaaaatct
//
LOCUS       DADOZM010000110         8940 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.113, whole genome shotgun sequence.
ACCESSION   DADOZM010000110 DADOZM010000000
VERSION     DADOZM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.113"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(80..748)
                     /locus_tag="J8I47_002196"
     CDS             complement(80..748)
                     /locus_tag="J8I47_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6323622.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            882..1169
                     /locus_tag="J8I47_002197"
     CDS             882..1169
                     /locus_tag="J8I47_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323623.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1585..3123
                     /locus_tag="J8I47_002198"
     CDS             1585..3123
                     /locus_tag="J8I47_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAZ6323624.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3120..4085
                     /locus_tag="J8I47_002199"
     CDS             3120..4085
                     /locus_tag="J8I47_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323625.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4072..5394
                     /locus_tag="J8I47_002200"
     CDS             4072..5394
                     /locus_tag="J8I47_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323626.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5457..5561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5635..6378
                     /locus_tag="J8I47_002201"
     CDS             5635..6378
                     /locus_tag="J8I47_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6323627.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6393..7385
                     /locus_tag="J8I47_002202"
     CDS             6393..7385
                     /locus_tag="J8I47_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323628.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7397..8134
                     /locus_tag="J8I47_002203"
     CDS             7397..8134
                     /locus_tag="J8I47_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323629.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8124..8846
                     /locus_tag="J8I47_002204"
     CDS             8124..8846
                     /locus_tag="J8I47_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAZ6323630.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2984 a         1522 c         1870 g         2564 t
ORIGIN      
        1 gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt aaattaaata
       61 aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt ggttcaaatg
      121 tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc agatacttca
      181 ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga aaatgtgtga
      241 gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa agacttttgc
      301 caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc
      361 ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct gtcatcaaac
      421 tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat aaatgtaaaa
      481 agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa
      541 acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc
      601 caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc agcttagatg
      661 gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg attccgcaca
      721 attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa aattttcagc
      781 aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa aaaaattatt
      841 tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca gcagacttat
      901 tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt ttggctcaag
      961 aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa tttatctttt
     1021 tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt ttgatgaaga
     1081 accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt tatggagtga
     1141 aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac gtttatgatg
     1201 tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt ataatattcg
     1261 ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg gctgtttctt
     1321 tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc
     1381 gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt
     1441 gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg
     1501 tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta
     1561 aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa
     1621 ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt
     1681 gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa
     1741 gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta
     1801 cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct
     1861 ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa
     1921 gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc
     1981 atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg
     2041 gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt
     2101 gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt
     2161 atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact
     2221 ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa
     2281 aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg
     2341 tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc
     2401 aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc
     2461 acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg
     2521 acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca
     2581 ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg
     2641 aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg
     2701 ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat
     2761 ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat
     2821 aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat
     2881 ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg
     2941 aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga
     3001 aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag
     3061 ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag
     3121 tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg
     3181 atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta
     3241 gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca
     3301 tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg
     3361 gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag
     3421 tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc
     3481 ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg
     3541 gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt
     3601 tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg
     3661 ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag
     3721 aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg
     3781 acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg
     3841 aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa
     3901 atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat
     3961 attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt
     4021 cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca
     4081 tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa
     4141 gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac
     4201 cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa
     4261 caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa
     4321 ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct
     4381 gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac
     4441 gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta
     4501 ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg
     4561 cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc
     4621 aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt
     4681 gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct
     4741 tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa
     4801 gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa
     4861 tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat
     4921 tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc
     4981 ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat
     5041 gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa
     5101 cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg
     5161 gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg
     5221 acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa
     5281 ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc
     5341 tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca
     5401 tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc
     5461 tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc
     5521 tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc
     5581 gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag
     5641 aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc
     5701 atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta
     5761 gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt
     5821 ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag
     5881 tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg
     5941 ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt
     6001 gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca
     6061 aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata
     6121 tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat
     6181 tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac
     6241 atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc
     6301 attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt
     6361 atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc
     6421 tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa
     6481 aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca
     6541 tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg
     6601 aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg
     6661 taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag
     6721 caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca
     6781 tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag
     6841 aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg
     6901 taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact
     6961 ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat
     7021 tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg aatgatcaac
     7081 tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc
     7141 aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa
     7201 atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc
     7261 ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg
     7321 aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa
     7381 aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct
     7441 gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc
     7501 gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac
     7561 ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta
     7621 tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata
     7681 tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt
     7741 tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg
     7801 aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt
     7861 tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa
     7921 aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc
     7981 agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc
     8041 tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta
     8101 tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc
     8161 agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc
     8221 cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg
     8281 gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa
     8341 aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta
     8401 cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg
     8461 tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc
     8521 actatttgat ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct
     8581 gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag
     8641 cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt
     8701 taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa
     8761 tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg
     8821 aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct
     8881 ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct tagtaccaac
//
LOCUS       DADOZM010000111         8856 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.114, whole genome shotgun sequence.
ACCESSION   DADOZM010000111 DADOZM010000000
VERSION     DADOZM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.114"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(335..418)
                     /locus_tag="J8I47_002205"
     tRNA            complement(335..418)
                     /locus_tag="J8I47_002205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Leu,seq:cag)
     gene            946..1446
                     /locus_tag="J8I47_002206"
     CDS             946..1446
                     /locus_tag="J8I47_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323631.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1636..1980)
                     /locus_tag="J8I47_002207"
     CDS             complement(1636..1980)
                     /locus_tag="J8I47_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAZ6323632.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2134..3054)
                     /locus_tag="J8I47_002208"
     CDS             complement(2134..3054)
                     /locus_tag="J8I47_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ6323633.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3241..3732
                     /locus_tag="J8I47_002209"
     CDS             3241..3732
                     /locus_tag="J8I47_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAZ6323634.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3756..6131)
                     /locus_tag="J8I47_002210"
     CDS             complement(3756..6131)
                     /locus_tag="J8I47_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAZ6323635.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6457..7083
                     /locus_tag="J8I47_002211"
     CDS             6457..7083
                     /locus_tag="J8I47_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ6323636.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7106..7810
                     /locus_tag="J8I47_002212"
     CDS             7106..7810
                     /locus_tag="J8I47_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ6323637.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8002..8835
                     /locus_tag="J8I47_002213"
     CDS             8002..8835
                     /locus_tag="J8I47_002213"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAZ6323638.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2684 a         1684 c         1563 g         2925 t
ORIGIN      
        1 gttggtacta agattcatta tttgaacgtt taaacacatt caaaatatag taagaggtca
       61 tgcgatttat tctttcgcat gacctctttt tgagtttaag tttagtcttt attccttaac
      121 tatcgtttgc cagtatttct tgtatcagaa tctataaatc tactatttga atcaatctat
      181 taggttttaa actgtcccac cttcacacaa attttttttg atttatttga atctggttcc
      241 gttgtttaaa atttttccta tattctttta gataatatgt tattttcttt ctcatcgctc
      301 caggtgtacc ttctctttat aaaaaaacaa attatgcggc taagaagact cgaacttcca
      361 cgaggtcacc ctcactagcg cctgaagcta gcgcgtctgc caattccgcc atagccgcat
      421 cacatctttt agtttaggat tacatgtgtt ttttgtcaag aacatatttt tgttcttttt
      481 catttctgta cgccctccct tatgccatct acacaaataa ttcatccgag caactccatc
      541 atctattgag ggttggtgaa aaaatctgtt ttatccatat gatcgatttc aatgttaaac
      601 gaacgactct gggattagta actattttat ttttttcttc atttcttttt tcctcttttt
      661 ctcgattcag ctatcttctt tcatttggat attgattatt tcttctgttg attactatcc
      721 ttctggaatt ctagacgctg aagtaattca cgttttcttt cttctagaga tcataagtaa
      781 cgaatgaacg aacttcaaat aattttttta ttgtaattca tatttgtatt gttcgatgat
      841 tcttaatacg atatgacaca ataaataaaa ataggcatgg ctatttacaa caatagtgct
      901 atagtagaaa acaaatgaat aaggttactt aaagaggtga atacgatgaa actgatcaaa
      961 ctatgggaag cagatttaaa gaaagcttat cagctacaaa ctagctttgg accaaatgag
     1021 agtggattta tcaacaatgc ttatggctat acatttgctg aatttttaga ttatgtaaaa
     1081 aaatgcgaaa aaacgtcttt aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc
     1141 tttattttag aaagtgatga acatgagtat gtaggaattt ttaatttgcg ccataaactg
     1201 aacgaaacat taagaaacgg cgctggccat attggttacg gcatctcccc taattatcgt
     1261 aaaaaaggat atgccacaca aggattagca ttagtactac aagaggcaaa aaaaatcggc
     1321 attgatgagg cacttttgtc tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa
     1381 aaaaatgggg ctgcgattta tcgtgaagat gagaaagaat actatacgaa aatcaaactg
     1441 atgtaaaaaa gaaggattcc aaaaggaaag ggatttgaat gtcctttatc aatagaacac
     1501 atttatttaa ttaacttata atatagcaga caaaatttta taaacaaaga aaccttttat
     1561 ctcttttctt ctagaaagga taaaaggctt ttttgttttt cctatttgct tctcatagct
     1621 agtcagccaa gatttctatc gtatccccaa cgcaatccgt gcataacggc tcatcttatc
     1681 tgtcgtccat gctggatacc agactagttt gacttctgct ttcgttactt ctggcacttc
     1741 ttttaatgcg tcatggatag attctgttaa aatatctgct aatggacagc ccatagttgt
     1801 tagagtcatc ttaatgactg tttctccatt ttcaggattg aattctatat cataaatcaa
     1861 gcctaaattg acgatgtcga ttcctaattc aggatcaatc accatttcca aggcagcaag
     1921 aatacgttct ttgattagtt ctatttcttg tccagaccgt tcttggtttg cttcgctcat
     1981 cgtacgacac tccttaacac gatttttatt tatcatactc tttttttagc tgcttgtaaa
     2041 tgagaattta gaagtaagat tctcgtttaa atttataaaa atgttcttat tagatgtaat
     2101 agattaaatt tattctaaaa taaacctgat tcctcatttt ttttgtgaac gattttttat
     2161 ttctggcaat acgccagttg ttcgatacat tttttgttcg ttttcatttg caaaccggcc
     2221 aaatactgaa tgagttggta aaatattgat aaatgcatcg tcgtcgattt catgaacgat
     2281 ctgatcaaga tcatacattt cgtagcgggt gatgaccatc atgatcatgt tcccttgttt
     2341 ttttgaatag ccaccgacag acggcagtaa tgtcattcca cgtaccatcc gttttgctag
     2401 ttcttctgtt acctcttctg gcttgacggt aacgatcatt gctgttactt tttgatgact
     2461 cgtgtggatg gcatcaatta cttgagtcat tgcataaatg gaaataatcg tatacaaggc
     2521 actttcccag ttaaagaaga agcctgcgat gactacaatg ataccattta gaatcagcat
     2581 gtagtttccg actgtttttc cagttgtttg tgaaagtaca agagagatga tatccatccc
     2641 accagttgta aagcccattt ttaaagataa tcctacgcca agacctaata aaacaccgcc
     2701 tactagggca ttcatcaaaa tattatcagt gatctgtccg actggaagca gcatcgtcat
     2761 caccgataca catacaacat tcacaaaact aaggatcgtt gctcgttttc ctactttgag
     2821 aaaccctaat acaaacacag ggatattcaa aagaaaaata aatgttcctg tgtctatctt
     2881 gatatgaaac cattcatata aaagcgagga aatgatttga gcgatcccat tcatccctgc
     2941 agaaaagaca ttggctggaa tcaaaaaata atttaaagcg accgcagcgg tcaatccggt
     3001 tatcaaaata accactattt tctttaatct ttcattttta tgatacgcag tcataatttt
     3061 actcacaagc acctctcccc tactttggta ttccaaatgt aagaataaca gtttatctat
     3121 taaaataaaa tacttcgaac tcaaatgaga agaattttag caaacaggta agaatctgcc
     3181 ctaatcctat gataagatag atgtatggta aatatttata tgtatatcag gagggttcct
     3241 atggcaaaag atgctagctt cgacattgta tcagaagtca atacagaaga agtaaaaaat
     3301 gcgattcagc aaacaacaaa agaattgtcc aatcgctttg acttcaagga ttctacagta
     3361 gaaatcaaat tagagaacag caatctagtt gtattgagcg atgatgattt caaaatcgaa
     3421 cagatcaaag atgtattatt tggtaagctg atcaaaagaa atgtgccaat caaaaatatc
     3481 catttttccg aatcagaaca cgctctaggt ggcaaagctc gacaaactgc cgatttggtc
     3541 aacggaatcg atcgtgaaaa cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg
     3601 aaagtaaaag cacaaatcca agaagatcag attagagtga caggtaagaa ccgtgatgat
     3661 ctacaagctg tgattgcttt attacgtaaa cttgacttac caatcgagtt gcaattcact
     3721 aactatcgtt aatcaaaaaa cgaggctgac gtctgtcatt cctcgttttt ttgatttact
     3781 gcttcttttg ataaccaaaa acgctgtatt ccttcttcca attccccaac acttcgtact
     3841 tctcttgctg tttgccaatg ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt
     3901 tgatctgcca atacaacgat tccttgatcc gccatgccac gaatcactcg accaccagct
     3961 tgcaagactt tgttcattcc aggaagctga taagcgtaag caaatccaag gtgttccttc
     4021 tcatcataat atgatttgat cagttcctgc tcatgattca tctgcggcaa acctacacct
     4081 acgatcatac tgccgatcaa tcggtcccct ctcaaatcga ttccttctga aaaaatcccg
     4141 ccaagtacgc aaaatccaat caatgtttca gcgggatttg tttgaaattt tgttaaaaat
     4201 cgttcgcgtt cttcctcatt catgtccgtt tcttggatta gcacattgct gtcgggatat
     4261 tgctgtctga atgcttccac cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa
     4321 taattcccga cctttccatc agcaaaagca tgaatcgtct ctactaattt tgccagactg
     4381 tgttcccttt ttcgataagt cgtctctagg taatttgcga tgaaaacttg ctggtttcct
     4441 tctgggaaag gactcggcag tttgtaagcc agactttttt gtccccccag tgtttcctga
     4501 taataagaga gtggtgaaaa actagcagaa aaaagaatac tgctttttcc tttatccaac
     4561 gactgctcaa gaaacaagct tggatcaata caaaactctt ttactatcag atcgcgatac
     4621 gttttttcga ctgttgtttc gtagtgatca tcataaaatt cactgacctt caaaaaatga
     4681 agcaaatcaa aataataagg aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc
     4741 agccattctt taattttttc gcttaattga taacctgcct ttactaaaga ttcagccggt
     4801 gctttttgat gatgataatc ccaatgatct tcttttgcta tttctcggat cgaatcaaat
     4861 tcttttaata gtttgttgaa acggcgatga agttttttga attcctttgg tatagcttcc
     4921 ttacttcgtt tcgttttttc ataagatagt tctgccgaat acatctctcg ggaacgattc
     4981 accaaattat gtgcttcgtc aatcaaaaac agatagtcct cgtttttttc atcttcaaaa
     5041 aaacgtctta aataaacagt tggatcaaat aaataattgt aatccccaat gattacgtca
     5101 caccagatac tgacatctaa tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa
     5161 gtttcgatga cttcacgagt gatctgattc tcatgattca aaagatccca taaaccttca
     5221 ttaatccgat tgtaataacc attggcatat gggcactgat ctggattaca ggtcgtctca
     5281 tctaaaaagc agatcttgtc tttcgccgtc aaagtgacac tttttacttc agctcctgta
     5341 tcttttaatg ctgtcatagc atcttcagct acttgtctag ttattgtttt agccgttaga
     5401 taaaagatct tttctccctc ttcttcccct acagctttca aagcggggaa taacgtagag
     5461 attgtttttc ctgttccggt aggtgcttct acaaacaatt tctgtttcgt acggatcgtt
     5521 ttataagcag ctaccgctag ttcacgctga cctttcctat actcatcatg ggggaatttc
     5581 aaagccatca aagaggcatt gcgtacagtg cgccagtttt cttgaaaaac aagccattct
     5641 ttataatctt cagtcagctt tttaaaaaaa gtttctaact gctctcttgt ctgatgttca
     5701 acctttctcg tgatcacttc ttctgccgtc tggaagtaag tcaattgcag atttatttct
     5761 tgtaaatcat gttcttgact atagatatag gcatacaccc ttgcttgatg aaagaaaagt
     5821 tccacttgtt ctggttctaa atcttcaaat cgtggttcgg aggttttgat ttcatctata
     5881 taataaatac cgtctctttt gaaaataccg tctgcacggc cttcaacgat caggtaatat
     5941 tcatctaatt gaactgttgt ttttaaaaag acttcttttt catagttttc tcccgcatct
     6001 ttttggagct tgcggtggat tctcgctcct tctaatgcag tatggttact ttttttacga
     6061 ttgtcgatac ttccacgtcg caaaataaat tctactaagt gccgcacaga tatttttgac
     6121 tgtttgatca tttccaccac ctctttctct ctaagattac ctgaaaaaac ctagtttagc
     6181 aaaaagagct ctctttccaa acataaaaaa acaccctgct gattaggcag ggatatagga
     6241 tatagaattt ggatttttgg atgaatctaa tgtaacaagg acatattaaa atgctatgaa
     6301 aacttacttt ccttctcttt agaatatcca aatacttttc atatagtctg ataattcaga
     6361 taagtaaaga gaaataagta cctgtttttg tttcctagca atgaatgcgt tatactaatg
     6421 gatgaaagaa gttttggcta tcaaggagga atgcacatgc atatcgaaag attaaaaaca
     6481 ggaatgattg aagaaaactg ttatttggtc tacaacgaag aagcactttt gatcattgac
     6541 cctggagaag atgcggaacg aatcaaacaa gaaatcaaaa aaacaaacca acagccggta
     6601 gcgattttgc ttacccatac tcactacgat catatcggtg cagtggaacc tttacgcgcg
     6661 tattatcaaa tcccagttta tgtcagccca ctagagcaaa aatggctagg tgatccgatt
     6721 ttgaatcttt ctggactcgg tagacatgat gatattgctg atgttattgt ctcgcctgca
     6781 gaatatgaat ttgaaatgaa aacttatact ttaggaaaca tgtcttttcg cgtcgtccct
     6841 accccagggc attctatagg aagcgtcagc tttatttttg atgattttgt cgtttctggc
     6901 gacgccctgt ttagaggaag tatcggtaga accgatctgt atacagggaa tttagaacaa
     6961 ttacttcata gtattcggac ccagctgttt gtattacctg atgaatttgc agtctatcca
     7021 ggacatggcg atgcaacgac gattgaacag gaaaaaagga caaatccttt tttcaatcgt
     7081 taaaacgact taggaggaga ataaaatgac agaaactaca ctttatatcg tacgacacgg
     7141 aaaaacgatg ttcaatacaa ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa
     7201 gcaaggacaa gaaggcattc attatttagg tcgcgggtta aaaaatgtcg acttttcatt
     7261 tgcttattct agtgacagtg gtcgtgcaat cgaaactgcc cgtattattc taggagaaca
     7321 ttctaaggga aaagaaattc cttattttat tgaaaaaagg attagagaat ggtgtttcgg
     7381 gtctcttgaa ggtggatatg acatggaaat gtggggtgtg atccctcgtg tattgaattt
     7441 taaaacatat gatgaaatgt ttaccacaga agtttctttt gaacaaattg ctaatgctgt
     7501 atacaactta gataccgctg ggtgggcgga aacttatgaa acattgcgag accgtgtatg
     7561 gacaggattt gaagatatcg cccatcactg cgaaaaaaat ggtggtggaa atgttttagt
     7621 cgtatctcat ggtttgacga tcgccttttt gcttaatctg atcgatccta aacagcctgt
     7681 tcgtgctggg cttttaaatg gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc
     7741 tatccacggc atcaatgata cgtcttatat cgataacggg aaaacacata gtcgcgactc
     7801 gtttctataa tggggatatt ttaattttta ccagagaatc ataaaaggca aagtctgaac
     7861 gagggctttg tcttcttttt tctcttcgtg tacttatctg ctttatttct caagtttttc
     7921 ttaatattaa aaaacagatt gggtttctca aaaaataacg gtatactata agatgtatct
     7981 taattgggaa ggaaggaatt tatgtttttt gcaaatatta tcaaagcagt gattctcggt
     8041 attattgaag gaataacgga atggctgccc atcagtagta ctggtcattt gatcctagca
     8101 gatgaattca tcaaactgga tgccagttca caatttatgt cgatgttcaa tgtcgttatc
     8161 caattagggg cgatcttggc tgtcgtcgtt ctttattttc acaagttaaa cccattttct
     8221 ccaagtaaat caacggtaga aaaaaaagat acgtggacac tttggtttaa agtgatcgtt
     8281 gcttgcatac cagccatcat cattggtttg cctttggatg attggttaga agcaaatttc
     8341 cacaagttct taccagttgc cattgttttg atcgtttacg gtattgcctt tattattgtc
     8401 gaaaaacgca acaaaaacaa aacacctcgt tggacttctc tgaatgactt tacgtaccaa
     8461 gcagctcttc ttgttgggtt attccaagtc ttggcacttg ttcccggaac atcccgttct
     8521 ggcgcaacga tcctaggatc tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt
     8581 tcatttttct tagggattcc tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat
     8641 ttagctgaag ggaattcatt caaaatggaa gaaactgttg ttcttcttgt aggaacattc
     8701 gtatcttttg tggtttccgt cttagctatc aaattcttac taagctattt gaagaaaaac
     8761 gactttaccg cttttggctg gtatcgtatt atccttggta tcattttgat tggttattgg
     8821 tttattgcaa tgtagaaaaa accagaacac ttctct
//
LOCUS       DADOZM010000112         8818 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.115, whole genome shotgun sequence.
ACCESSION   DADOZM010000112 DADOZM010000000
VERSION     DADOZM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.115"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(138..428)
                     /locus_tag="J8I47_002214"
     CDS             complement(138..428)
                     /locus_tag="J8I47_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323639.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="J8I47_002215"
     CDS             complement(546..806)
                     /locus_tag="J8I47_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323640.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="J8I47_002216"
     CDS             complement(943..1356)
                     /locus_tag="J8I47_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAZ6323641.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="J8I47_002217"
     CDS             complement(1812..2297)
                     /locus_tag="J8I47_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323642.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="J8I47_002218"
     CDS             complement(2436..2651)
                     /locus_tag="J8I47_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAZ6323643.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="J8I47_002219"
     CDS             2875..3675
                     /locus_tag="J8I47_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6323644.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="J8I47_002220"
     CDS             3694..5562
                     /locus_tag="J8I47_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ6323645.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="J8I47_002221"
     CDS             5555..6046
                     /locus_tag="J8I47_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAZ6323646.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="J8I47_002222"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="J8I47_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAZ6323647.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="J8I47_002223"
     CDS             6606..7601
                     /locus_tag="J8I47_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAZ6323648.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7743..>8818)
                     /locus_tag="J8I47_002224"
     CDS             complement(7743..>8818)
                     /locus_tag="J8I47_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAZ6323649.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3047 a         1425 c         1682 g         2664 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg agtttagtct aatggtgata
     7621 agtcaagatg aaatttaaga ttttctttat tgagtaatat gttttgaagc gcacgaaact
     7681 agacccgatc aaaatcaaat ttttttcggg ttgattcaag gatttgcgtc aataacggat
     7741 tgttatttct ctatcacact ccttggtggc gtatagagtt ggtgtttacg tagtagcgta
     7801 tccaccagac gcgtaaattt tcttgcggtt aggacgagtg cacgtttgtg ttgatgcttt
     7861 ggaacctcct tatatttact ttgataaaaa gctttgtatt caggaagata attttttaca
     7921 gagttggcag cttcaactaa gtagtatcta aaatatcgat ttccttgttt tgtcagaggt
     7981 gtattttgcg actgatagtt tccagattgg ttcactttcc aacttaatcc agcgtatttt
     8041 gccaattttg tctgatcttc aaatctttcg atttgaccaa tttcagcgat taaaccggca
     8101 gcatagactt taccaacgcc tggaatactg gttaagcatt gatattctgg aatgacaaca
     8161 attacttgtt cgatggcttt atctaattct ttgatatttt tttcaagtgc tcgtatttct
     8221 cgtacgagaa cacttaaaac aacattaatt gattcttgtg caagagcacc taggcggtaa
     8281 ctcatggtaa ttgcacgttg aattgcttta gcgatttttt ctggttgctt gaaacggcct
     8341 tttccttttt cttgaagtaa atcacagaaa gcttctaaag gaagctcagc aagttcatcc
     8401 aatgtgaaat cttccgtcat gagtgaaatg agggttgcac taaaaagact cgtcgattcg
     8461 tctcgcattt ctcgagcaag cgtattacat ttgtacgtta gattttcaag aaaatgttgc
     8521 tttgtacgaa tcaattgctt aataagttga tagcgagtac gtgttaaacg ctgtaaggcc
     8581 atgtatttct cttctttgat aggggaagtt gtgaatcgtt gaatacgcaa gaaatcagcg
     8641 attcttaatg catcgtttcg atcagtttta ttctcatcaa acatgcgact aaattgtttc
     8701 acccgaaaag gattttcaat cgttacaagt gtgttgagct tctttagttt ctcatcttca
     8761 tgaaaaaaca tggaaggatg gaagctgtac atggacgttg actccatgcc aatcacaa
//
LOCUS       DADOZM010000113         8608 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.116, whole genome shotgun sequence.
ACCESSION   DADOZM010000113 DADOZM010000000
VERSION     DADOZM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.116"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(74..1030)
                     /locus_tag="J8I47_002225"
     CDS             complement(74..1030)
                     /locus_tag="J8I47_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAZ6323650.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="J8I47_002226"
     CDS             1134..1736
                     /locus_tag="J8I47_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ6323651.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="J8I47_002227"
     CDS             complement(1824..3323)
                     /locus_tag="J8I47_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAZ6323652.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="J8I47_002228"
     CDS             complement(3339..3863)
                     /locus_tag="J8I47_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323653.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="J8I47_002229"
     CDS             4161..4910
                     /locus_tag="J8I47_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAZ6323654.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="J8I47_002230"
     CDS             4901..5686
                     /locus_tag="J8I47_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ6323655.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="J8I47_002231"
     CDS             5655..6371
                     /locus_tag="J8I47_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ6323656.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="J8I47_002232"
     CDS             complement(6473..7492)
                     /locus_tag="J8I47_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAZ6323657.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="J8I47_002233"
     CDS             complement(7511..8569)
                     /locus_tag="J8I47_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAZ6323658.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2582 a         1752 c         1543 g         2731 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tggcgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt cggtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatgg
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggcca gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaa
//
LOCUS       DADOZM010000114         8516 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.117, whole genome shotgun sequence.
ACCESSION   DADOZM010000114 DADOZM010000000
VERSION     DADOZM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.117"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..195)
                     /locus_tag="J8I47_002234"
     CDS             complement(<1..195)
                     /locus_tag="J8I47_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323659.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLMILFVKLS"
     gene            complement(615..1736)
                     /locus_tag="J8I47_002235"
     CDS             complement(615..1736)
                     /locus_tag="J8I47_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAZ6323660.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2064..3473
                     /locus_tag="J8I47_002236"
     CDS             2064..3473
                     /locus_tag="J8I47_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAZ6323661.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3470..4198
                     /locus_tag="J8I47_002237"
     CDS             3470..4198
                     /locus_tag="J8I47_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6323662.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4326..5237)
                     /locus_tag="J8I47_002238"
     CDS             complement(4326..5237)
                     /locus_tag="J8I47_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6323663.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5254..6261)
                     /locus_tag="J8I47_002239"
     CDS             complement(5254..6261)
                     /locus_tag="J8I47_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAZ6323664.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6258..8387)
                     /locus_tag="J8I47_002240"
     CDS             complement(6258..8387)
                     /locus_tag="J8I47_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ6323665.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8392..8499)
                     /locus_tag="J8I47_002241"
     CDS             complement(8392..8499)
                     /locus_tag="J8I47_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323666.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2608 a         1731 c         1488 g         2689 t
ORIGIN      
        1 gcttaatttg acaaataata tcattaaaaa gttagtaaat aattgccctc taagttccac
       61 ggtctgttca ttaataactt tatatcctcg tttttcaaaa aatggctttg cggtaatgga
      121 agcgtgggta gtgatttttt gcataccagt ttctaaaaca tggttttcca attcatctgc
      181 aattttagtc gcaatacctt ttccttgatg gctatgtttt tgtcacaact tcagtattcc
      241 tgtaacaata cggatttcag tatacaacaa cagcttttga aaagatactt gcaaaaggat
      301 taagatatct atgacctgct atgctcactg atgttgaaaa gaatgtttta ttaggattga
      361 ggtaagcaag atagtgaata tgtccggaaa gcctgtaaaa taaggctttt cggacatatg
      421 tgaagttata aaaagatagc gaaaaatcta tttagtatca ctaatgttgc gaacatatcg
      481 tcagaatagt ctgacattta tttcttgcta aaaatttcaa aaaagtcaag tgctgacgca
      541 gcggatgact attttccaga aaattttagt atgtattcaa aaatcaaacg tgacaacagc
      601 tattaactac atcctcatcc aggtctgtaa cgtttgaacc actcttctgc gggctcaaac
      661 attaagtaat ggaaggtacg tttcaattga aacagcgtcg ctgtcgtatt caactcaatt
      721 tttatcacat aagtgagaag cgttgcgatt aaggtgagga tgacttgatt tatcgcccct
      781 tgttcactat gtgaatatag tcgtttaatg gtcagatttt gttttaagtg tttgaacaac
      841 aattcaatct gccaacgggc ttgatacatg tcggccactt cgtttggcga tacatcaaag
      901 agattggtca ccattcgcag gattttttgt cccttggtaa gaaccgttac caacctaaaa
      961 cgctctgtca catggttgac accgccgaca agaatcactt gttggtcact gattatcgtc
     1021 ccatcacggg ttgttgtatc tgctacttca atttgatcca agatggtaat ttttgtatta
     1081 gattttgtcc gtgtaacgaa gaaatagccg tcacgattta gtttgtctaa caacttgtaa
     1141 tcaaagtagc cgcgatccac cacatacgtt gcttgcgttt tatcaacaag aacttcaagg
     1201 tgattacggt catgctcact cgcatttgtc atcgtaaaac tttcaggata ctgattggtt
     1261 ttatcaagat aacagagatt caaatgtagc ttgatgccag actttgtttt ccgaaacttg
     1321 gcccatggaa accatgcttt gttcaacgga atagtcgttg aatcaatcaa ttgtagactg
     1381 gttgtttttg agcttggctg ctgagcagaa attctggcga ctaattgcgt aaaaatttcc
     1441 atcaatactt cttgcttgat ttctggcgct ctacgagata acgagctgta acacagggaa
     1501 tcaatgccaa gttccttgca gaggcgctga tctaaaaagg cgcgatcaat ctcgcgatag
     1561 cttggcaatt cttcataaac cgcatgaagt aacactttaa gcgtcgtttg gaagtctagt
     1621 tttttactgt agaaatcaaa gtcgctaatt aagctttgag agttttctga taaaccagaa
     1681 aaatttattg cagaaaacca tttttgaaat gaggttttta cgttatactt atccatgcgt
     1741 gagtcctttt tattggtctt ggataagtca tccgagaata gtataaagga cttttttgcg
     1801 tttggatagc tttgtattag gcttttgatg ggttaaaaag tctttgcaac actagtaatt
     1861 tagtatttat gtataaattg aaagatattt taccaccact tagtcttctt aaaagtgatc
     1921 caacattttg tttgtggcag aatttaacta cacactaatg cagccgaaag tacacaacta
     1981 tgcagcagtt tgactgcgaa ctggtttcct tccatcataa gtttgaatga cttagtgatg
     2041 gaggtacaga aaggttgtta actttggatg atattaaaac aataaaacat ttgagaaata
     2101 atcgtgatca gtcaattgat cagataagaa aagcaatgaa tatcaattgg cgaacagcga
     2161 aaaaatacgc cgatagtgat caactattca ttacagatcc cactcgaaaa aacaaatcca
     2221 tgatggatga tggttgggga gaaatcatcg gtagttggct agaagaagac ctacgtttac
     2281 gcagaaaact tcgtagaacg gccaaagcta tccatgaaca attgaaacct ttgggtttcc
     2341 ctggctccta tcgaacagtt tgtgtataca tttctcaatg gaaaaagcag cgttacgacg
     2401 aaacgaatta ccaagctgtc gatcgtcttg atcatccgat cggagaagca caggtcgatt
     2461 ttggaacaat ggaagttatg catgaagatc acatgaaaga tatacaactt cttatcatgt
     2521 catttcccta tagtaatgcg acgtccgcaa gtgccatgcc agctcaaaac caagagtgtt
     2581 tcctggaagc attaaaacaa ctatttaatc aatgcggcgg cgttccaaaa acgatcagaa
     2641 tagataactt aactccagcg gtgaaaaaaa caaaatcaaa atttgaagaa gcacaactaa
     2701 ccgatgaatt cttacgtttc accattcatt atggttgtga tgttcaagtc tgtaacccac
     2761 gtagtgggca tgaaaaagga catgtcgaaa ataaagtggg ttatgtccga tacaatttct
     2821 tctcgacttc gccaatcatg gactcttttg agtccctaaa taagcaactt gaagaacaat
     2881 taaaagcaga tcgaaaaagg acgcattatt caaaacatca attaatcgaa gatttgtgga
     2941 agaaagaaaa taaagcgtta cattctcttc cacaagagga ttacccaatt tttaaagaag
     3001 tggaagctaa actaaatcgt tatcacgaaa tgaaattgga tcaaacggtt attcatattc
     3061 ctaaagcata tggctattct caattgactg ttgttttgac ttggaataaa ttcaaagttc
     3121 ttacgcctca tggagaaata ttaatgaacg aatatcgccc ctatatgaac aaaaaaagat
     3181 tgatcccatg gagtgacatc atgaaacaat ggagtaataa attgaatgtc gttagttatt
     3241 cgaggtattg gaagtacttg cctgcacgcc ttcaaaatta tctagcgatt gataataatc
     3301 acctgaaata cagacggata aaacaattac taagtttatt agcgacctac gatatccaac
     3361 aaattaatga acaattttac gaattagtcg ttcaagaaga cgccccctct gactttgatg
     3421 tgaattggtc aaactatgat gcattaacaa aaacaggagg tgtgcaaaaa tgattggaca
     3481 gacgttagaa gattattgta aaaagcttcg attaagccat atcatcacga acataccaag
     3541 agcttctttc gaagctccag aacaatttat taccgattta ttccgtttag aactggagac
     3601 cagacagcag atcaaattag aacggatgga aaaattagcc aaatttcctg taccaaaatc
     3661 gctaaagaac tatgaatggc acgaagatat ttttctacca tctcatctag ataaggagac
     3721 ccttgtctct cttgattttc taaaaaggca agaaaacatt gtttgtgttg gctctcctgg
     3781 tacagggaaa acacatctag cgatcgcatt agggagaaaa gcctgtagtg aaggtgtttc
     3841 aaccaaattt ttccgagtta acaatctcgt agagcaatta gagaatgcat taaaaaaaca
     3901 acagctagcc agtttcaaga agcgattcga aacaacagaa ttaattattc ttgatgaaat
     3961 ggggtactta ccattgacaa aagaaggagc tgaacttctc ttccaactga tttcagattg
     4021 gtatgagcaa aagagcatta ttattacctc taatttagaa ttcagtcaat ggaatcgagt
     4081 gtttatggat tctcgattaa cagctgctct cgttgatcgg ctaatccatc acgctcacat
     4141 tatcagcttc aaaggcgaaa gtttcagatt gaaaaattca ttatctaaat catattgaag
     4201 gaaaaaacta gttgcagcaa aactctgcac tttcggctgc atttttttgt aaaaagctat
     4261 tgcacaacac aaacatttga acgacgtaat tccaaatgtt ggattgcttt tagataatct
     4321 tttatttatt cacaattgct aatagtccaa ttttcatttt tgtatagttt taatctatac
     4381 tgaaaaacac ttgttgtttt ggacaactca tcaagatact caacaccaac aatcacttga
     4441 atcgctttac ccttttgttt gtaaatagga ttcactaatt ccacaaattt caaattagta
     4501 ttaattggtt gcatcgcgtc tttttcaaca taatgctcta gttctttttc tgaggctgct
     4561 ggataaagcc tgaaaaacgt ctctaaaaac tcggtgattt cttccgttgt ctttgaatca
     4621 atttttgaat cactttttac ttgtcttact ttatgttttg ccttttctgg cttaccaatc
     4681 atagtgggta aactcgttac aatatgattg cctttttcat cttcaaagat catcagacga
     4741 tatgcagaag aaattgattg tgtctttttc ccttctgtaa tcttttgatc taccacaaat
     4801 gaaacgacaa attcttccga gtgtttttca acatctagaa tttttactga tacaacctcg
     4861 gaactagtgg gaatatccgc tcgaaccata tcttggctca aagcgagccc ttcttctgta
     4921 aggtactgct caagattagt cattctcttt tcaattacct ccttgttatt cttccatgaa
     4981 aaatattctt tcacaaaatc tacagtaaaa ttttctactc cagaggtatt cactattttc
     5041 tctttcacga ccactttctc atgagttgta tgctgatcaa tggccgtgaa gtttttgtag
     5101 attccaaaag caaggctaca agacaataaa agccaaaggg caagcaccat ttttcgatgc
     5161 ttgcccacgg atactactcg ttctttcttc ttttttggaa tcttttcctt gttacttcgc
     5221 tcaattttta tcttcatttt ccattcctct tttctattgt ttataccgac cagcaccaat
     5281 aatgtgctgt tgccaatagc tatcggtaag attggtccaa cccaccggat ctcctgcatg
     5341 aaacattctg ttctctcctg cataaatgcc cacatgagtc acataagtac cggcatcata
     5401 agttgaatga aagaaaatca aatcgcctgg tttggcatct tttaagtcaa tatgagtcat
     5461 agcatcatat tgatcctgag ccacccgagg gagtttcaaa ccaactttgg cataacacca
     5521 ttgagttaaa ccactacaat caaacccagt tccgggactt gacccaccaa atacataagc
     5581 cgttccttca tacttgaacg cctcgtcaaa aattccttgg actgttttat catcaaactg
     5641 tgttgtagtt aggtactgct tcactaataa gcaataaaac atattgccat agttataccg
     5701 ccacccacta ttttccttaa tcgcgatggg attgggataa tcgacttttt gaccacccga
     5761 tttttctttt gaaaaagatt cggctaaagc aaaactatgc ttctttccgt ttttcgcgac
     5821 ataatccaaa tacccgtcac catagttata agcctgaatg atactatctt gatcgcatcc
     5881 tagttctttg gaacacttaa ccaaagacga aaaatagaga cacacttgtt tgatggatgc
     5941 ctcggtactt aatgaattcg gtgataaacc tgctgattca gaactttgca tgacatcttc
     6001 cattgtacct ccagattcga cttgaataat cgccaaaaga atcgaaacat actctgttat
     6061 atctgattct ttggcatact tttccaccat tgaacggtgt ttcaaaacta cttctgagac
     6121 actactacct ccaggagaag ttccagaact accacttccc tcaccgtcct caccaaacaa
     6181 caaacccaca cacatcaaga tgcccacaaa agagatggtc aggaaggtta acgaaataat
     6241 actcaatttt tttaagttca tcttttctca cctggctttg gttttgggcg agtgttcttt
     6301 tttctttgta cttgttgatt ggttcgcttt ggaaaagtga atctctgttt cacttgttga
     6361 aaggaattgt tcttgcttag tggctgagtg cttccgtttg gaatgacttc ttgactagaa
     6421 agtttccgtt tgatttcagg attcgaatgt acttgtaccc ttggttttaa agacctattt
     6481 tctggtagtt catttttctg tggttttatc tcctttttta tcggaacatt ttcccttcct
     6541 ttctcatgac gattccatgc ttcgttcaat actttgcgtt tttcatccat ttttgcgtct
     6601 cttcgttgtc gttgtttttc tcgcaactcc cctcgatttg ctttttcttc gaaaggccct
     6661 cgcttaaagt cctctggtac ttttttcgtc ttttccatgc ctttatgaag gctgtatttc
     6721 aagttagttg gtgcatcggt tacttgattt tttgcttgtt tcgctttgtc cttcacgatt
     6781 tcttttgtat cgagtacttt tccaactttt ctaccaacta aatttattcg tgattctttc
     6841 ttcgtgccca ctggttgttc ttgttttttc agatcagaat ttagctcgtt aatgcttctc
     6901 gcggaatggt tctttctatt ggacactcta ttgatttttg aactaccttt cccttttgta
     6961 gaactggtag ctagtttccc accaactgct gcaccgccac caaaagcaaa agttcgaccc
     7021 acatttcgat taagctgtcg caattttcga ttcatcaaca tttgcggttt ccgcatgact
     7081 cgccttccca ttgactgact atcattactt tgaaggttaa acatgctcat aatgtcacct
     7141 aatttgaaat agataccggc aaaggtcaca atttgtagaa aagctaccat aaagaaggga
     7201 taggtggccg aaatattgaa aaacatcgtg gagatactga atgcagtcgt aatgactaag
     7261 gtaactcccg ctcgcatcat aatcgtattg aacaaacgaa taatcgccct tttgcccaaa
     7321 ctctcatacg tcggcaacat cgacaataag aaactaatgg gcagaaacat cgcaaagata
     7381 atgaaaagga tttgtgagaa caacataatc cctgtcagaa gaaacacaaa aaaggaaatc
     7441 ccaatattaa acaacactaa aaagaccacg gttcctaaac ggttcatggt cttggtaata
     7501 ctgagattcg cattgtcatt atcttcaatc tctgctttga ctgcttcttc ccgatcctta
     7561 cccttatttt catccggact cacggataaa atcttattaa cccgaccctc gccaatttct
     7621 tctatattgg aatcatcgaa ttgtaatagt aaccagggtt gttgaacttg aatcgaaaat
     7681 aaactgtctc gaatcaaatc cacactatct tttccctggc tttctgaatt aggtacaaca
     7741 atcttggttc caaggctaag agccgcttca ctaacgtctg agctaaagtc attgattttt
     7801 gtaatatagg tgggcgcata cgcaatgaaa gaacccgaga gtagaaaaat catcacaaaa
     7861 ttaatgacgg ttcggactgc tttggtagtt tctcgtttta ataatccggt atacgccaca
     7921 taaactccta gagctaaaat catcaataat aggaacccaa agtagaatcc cgaagtctgt
     7981 aatccatttt cagtgatacc ggctaaagtt tggatattct ttccaatact tttagccgta
     8041 tccgaaatga aatccaactt gtaagcttct tgaaccacat agcccgtggc gtttgataaa
     8101 tacagactta ctgtccaaat aaaattggta atggcataaa gaccatacat cacgcttttc
     8161 cctaagccat ccccccaatt ccaaggtaac caatcccaag aactatccac aaagaaatcc
     8221 agttgataat tgttcagaga atatttggaa taaaggtttc cggcttcaac tgtatcatca
     8281 accagacctg tcgcctcagc taccgtaccg actaaactca atagaactgt cagagtgacg
     8341 atgctaataa aaacgattcc cacaatccga aaaagtttct ttttcatccc ctcactccat
     8401 tctctcttga accggcggac gagtgtcaaa agcatggaag agttcttcaa agactggatg
     8461 aacttgaacc acacctactc gcccatacaa gtcttgcatc aagcattgac cgtttt
//
LOCUS       DADOZM010000115         8183 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.118, whole genome shotgun sequence.
ACCESSION   DADOZM010000115 DADOZM010000000
VERSION     DADOZM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.118"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(84..284)
                     /locus_tag="J8I47_002242"
     CDS             complement(84..284)
                     /locus_tag="J8I47_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6323667.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(737..1057)
                     /locus_tag="J8I47_002243"
     CDS             complement(737..1057)
                     /locus_tag="J8I47_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323668.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1560..2678)
                     /locus_tag="J8I47_002244"
     CDS             complement(1560..2678)
                     /locus_tag="J8I47_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323669.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3149..4456
                     /locus_tag="J8I47_002245"
     CDS             3149..4456
                     /locus_tag="J8I47_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAZ6323670.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4577..5776
                     /locus_tag="J8I47_002246"
     CDS             4577..5776
                     /locus_tag="J8I47_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAZ6323671.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5803..7071)
                     /locus_tag="J8I47_002247"
     CDS             complement(5803..7071)
                     /locus_tag="J8I47_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAZ6323672.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(7241..7882)
                     /locus_tag="J8I47_002248"
     CDS             complement(7241..7882)
                     /locus_tag="J8I47_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAZ6323673.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
BASE COUNT         2509 a         1699 c         1428 g         2547 t
ORIGIN      
        1 tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa atgtctgggc
       61 gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg aggtccgcgt
      121 tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt gaatccgtcg
      181 ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt gatgaatcca
      241 aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa aatcctcctt
      301 gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga tgtttatttg
      361 aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca taccgtttac
      421 aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc gattacataa
      481 agtgaaaaac cgaacttttt tttaaaataa acgacatatt gttcagttgg tgtttttttg
      541 ctctgatgtt cttttttaaa ctaaaaaaat tattctttct gactacttat cggttctgtt
      601 tcaacggtcg aggctgggga agattcttta gtaggtctga tgttctcatg gtatttctga
      661 tagcctatga cagttaccag aagaacaagg caaaccaata cgaccaacga gattagtttt
      721 tcttttttgc tcatatctat acctcataaa taacaggatg atgtccatca tatttctgag
      781 tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa atgccagcaa ctttcataat
      841 cacaggtcgg acagtaccaa aagtaaccaa atcttggctg caaagaccgt tcttctgttt
      901 caggaatcgc agaatcaaga tctggtgtct gtacataaac atttttgtac agacgtgtgc
      961 ctgtaatagg aaaccgctgt gtttcttcag caaaaactgg ctgacttgtt aaacttaggg
     1021 atagagacag caaacaccat aacagtcttt ttttcataaa gacctcctta tcgataagtt
     1081 tttcccaatt aaaatcatat ctcaagaacg cccgttattc acattttgtt cataaatgat
     1141 gttttttgta tcataatcgt caaaaagagg tcgtgaccac gtcacaacct cttttttaaa
     1201 cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaaat
     1261 aatttttgtc aatctcttct tatttcttga agctgaccac ttctgtcaca acctcttcct
     1321 taaacggtgt tcaaaagctt gcatctgcgg taataagccc accaaacaaa aaatatgaag
     1381 agctattttt gcttgctgtt cttattactt gatgcagaac agctttttca caacctctca
     1441 tacacggtgt ttaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgtgaa
     1501 actatttttc cgtttgtttc tctaatactc aggtcaaaac actgatttca caacctcttt
     1561 taataacgaa gaatcgctgt cagactcttt tcatctggcg catctttttg atccaaaata
     1621 aacacttttc cgccattaat gatcacatca tctgctaatg cattcaacac ttgacgccga
     1681 tcatattctg tttcaggatc ttcaccgaaa ccatcagcta agtttgacgt agcgataaat
     1741 aaatgtgaca cttttccttc tttcgcagct ggaacgatat caactaactg atcgatgaat
     1801 ttcttatcta agagtttatt gtattctttt gattcttgag ctgcgagttc acttgacagc
     1861 ttcttcgccc cttcttcgat atctctaaag gatagctgcg caggagaagc agaaatcgca
     1921 acgtctgcac aataataggg atttttagca atttttttga acatcgtctg attttctggt
     1981 agagcaaata aataaatcgg ccattgttct ggattttcga aagaatcctt caagtacgta
     2041 tcaactgctt ggtaatagtt gacccaatcg atctctactt cttcatcttt tggatttacg
     2101 ccatggaaag ttactccatc tcgggctccg cctcctccat tagaagagaa gttcaaaccg
     2161 ccacctgtca gttcagatcc caatgcagta acgacatcaa ccggtgcccc ttctggtaga
     2221 gatacttctt ttacttgttt gttgctgact tcatacaatt tcatggagtc gcggttcaat
     2281 cccaataaat aataagaata gttgaactgc ccatttttga taatagccag taaataagga
     2341 agatcactta catagtattg atcgtctacc gggatactca aacgatggat ataggtttgg
     2401 tcagcagtta taatgaccgc tacactcgta gttgcactac gccaaaacga aggatctcct
     2461 aacaaatttt caattttttc ttggaacggg gaccagtcat gttcggaata ttttttctca
     2521 aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga tctgatcttt ttccacatct
     2581 tgatgggcga catgcgtatt cagcatgatc gtgacgaatg gtcctttagc gccttctgaa
     2641 gtcaattgtg acaataattc tttatccttt tttgccataa acgtacccct cctaaaaaaa
     2701 tcattccttt gtttctatct aaagtctacc acaacactat ttttccatac aataaaacgt
     2761 atctaaaaaa ataaaacggt ttctacggtt ttaaatttag ctattttttt aagttatctt
     2821 cataatttcc tcaattattt agccccagtc ttcaactatt cttcatgaaa tgctgataaa
     2881 ctaagaaaaa gaataaaaga ggtggatctt atgcatttga ttatgatttt cagtttatta
     2941 agtattatca gtttatttgc tttcagcgct tcccgcttat tagatgcgga aacacagcct
     3001 attcctgtaa aagtacgtaa aaacgaccgc taaccttcca taacgtgcgt tcttatccta
     3061 aagttttttc agaaacattg aaactctctc ccctctctga taaaatttta atttgtagcg
     3121 aaacaaatta attacaagga gatcggatat gtccataaaa aaaacacgtc tagtcctatt
     3181 attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg tgcaagctga
     3241 agatactttc aaagtaaatg caaaagcagc ctttgctgta gatgcgcaaa gcggtaagat
     3301 tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg
     3361 tctttacatc gtccttgatc aagtaaaaga agggaaacta tcatgggatg ataaagtttc
     3421 aatttcagat tatgcagaaa atttgagtgt caccccagat ttatcaaatg ttcctttgca
     3481 taaagaaaac acctacaccg ttaaagaatt attcgactct gcaatcatcc agtcagccaa
     3541 cgcttcaatg gttgccttgg cagaaaaaat ctctggcagc gaagcaaaat tcactgaacg
     3601 aatgaaagaa caattgaaag attggggtat caaagatgca acgatcgtta atgcatcagg
     3661 actgaataac tcttatcttg gcgaaaaccg accagaaggt actggagaaa acgacgaaaa
     3721 tcaaatgtct gctcaagatg tggctatcgt tgcccgtcat ttgatcttgg attttccaga
     3781 aatactggat gtatctagca ctacgacaca aatgtttggc gaaaacacgc aatcaccagt
     3841 agaaatggtc aactggaact ggatgctccc aggattcgtg aattataaag aaggcgtgga
     3901 cggattaaaa actggtacga ctgaacttgc aggagcctgt ttcgtcggta cgatcactaa
     3961 aaacgatcaa cgaatcatca ctgtcgtatt gaatgcagac ggccatgcgg aaaacccaag
     4021 tgcccgtttc aatgaaacag ctcgcttaat ggattattgc tacgacaatt ggtcagaaaa
     4081 agagcttgga aaagccaatg caagtatccc tagtctaaaa accattgatg taaaagacgg
     4141 aaaagaatca acagtgcccg ttgtgcttaa aagtcctgta aaagtatggg tacggaacga
     4201 tatggatact ggaaaattaa caatcacacc aactcttgac aaaaaacaag tcactgacaa
     4261 tgagatccaa gcacctatgg ataaaggaac aaaaatcggt accgcaacga tcaccttggc
     4321 agaagataaa ttaggttatc ttgaagacaa caacgcgcca tccacagaaa tcatcacgga
     4381 taaaaccgta gaaaaagcaa atattttcgt tattggctgg cgacgcgtcg cagaattttt
     4441 cggtaacttg ttttaattga acaaaaagaa aaaagcatct ttccatgtgg aagatgtttt
     4501 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     4561 aaaaggagtt gaatttatga atctaaccag agatctgatt tttttggttc tctatcttat
     4621 tggggtattc tccatcgttt attggcgaaa acagaaaaag gatccttcct tgttctttta
     4681 cagcagtaca tggacgtttg cggtaatctt gacttatttt gtcgtgttgg aaatttttta
     4741 ttcgaccttt tattttttga ttcccttagt gtttttcagt atctttgcct tttcctattt
     4801 ttcagaaaag cgaaggctgt tgaatggtct tttattcaat attttcctca ttagttttgg
     4861 tatctacttg tttgtcttgc tatacgaaac acaaaatatt tttcttggcg gactgattgc
     4921 cttgatcacg atcccgttac tgcttgtctt tctattcggc atttatggat tgatcgtttt
     4981 tcttttttgg aacggtgtca cagttttacg tagagagtcg cattcattag ctaatctttt
     5041 aacgttgata ctagccattt ttcttacatt gtttcttgtt tttgactttt tcttgttaaa
     5101 atatttacca caatggataa atgctttgtt tttctgcatc ccattgatct tgatctatct
     5161 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accgccctcg
     5221 atacaaccaa gacttcatcg ttgtgttagg cgccgggttg atcaatggag aaacagtttc
     5281 tcctcttctt gccaagcgaa tcaataaggc gattgctttt tattgggcac aaagccgtgc
     5341 gacattaaat ccgcctatcc tattaatgtc gggtggacag ggagcagacg aaaaagtacc
     5401 agaagcaatg gcgatgaaac aatatgccat ggaacaagga attcctgaaa gagatattct
     5461 tgtcgaaacc aattcgacaa ccactttgga gaatatgcgc tattcaaagg aaatcatgga
     5521 tcagcaaatg aaaggtccct atcgcgcgat cttcagttcc aacaattacc acattttcag
     5581 agctggtctc tatgcacgac aagcgaaact gaaggcaaat ggcattgggg caaaaacagc
     5641 tttctattac ttgcctaacg cgtttttaag agaatacatc gccatcttga tgatgaacaa
     5701 aaaaagacat atgatcattt gcggattgct tttcgcaggt tccgcattct tatcactgat
     5761 cactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     5821 ttttattctc ttgacgattg gcaagaatag tattcctttt ttgacggcta cttgctgtgc
     5881 agaataaata ccgctgtttc ctgaacacat aaagctgatc aagcagatca taaagaagta
     5941 agcagcagct tgtgaaccaa ataattcgat tcccatcaca aaacaggcaa tcggtgtatt
     6001 tgtcgcacca gaaaataccc cgataaaacc taatccagca agaaaaggaa cagaaacatg
     6061 gagcaaaggc gctagtactg cgccaagcgt tgcaccaatc tcaaatagtg gtgtcacttc
     6121 tcccccttgg taccccgcac ctaaagacaa gaccgtgaag aataatttcc caataaaatc
     6181 ataagcatgt gcctgtccat taaatgattc ctgcagtaac ggaagactca acccaaggta
     6241 acgttgtgtt tgcaaaatca acgctgcggc tacaactact gctccaccta tcgtatttcg
     6301 ccaaacagga tgaggaatcc attgcgcata ccgcgctttt aaaaagacga tcgaacggct
     6361 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     6421 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     6481 tgtcatgaaa tttgcaaaaa gcgcagcaaa gaagctagga aatattgctt ctgttcggac
     6541 tttaccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     6601 agaactaaaa cccgcactga ttccactgat aaccaaaatc tcccgttctg ctttatccaa
     6661 tcggaataaa tgcgctatat tatcagctaa tgctcccccc atttgaactg ctgttccttc
     6721 acgaccaaca gatcccccaa ataaatgggt agtgatggtc ccaaacaagg tcaatggaat
     6781 gagccgcaaa gggatcttct cttctcctcc attcccttga tcgatcacta agttattccc
     6841 acgactagaa agaccgccat gatacgcata aagataagca aaaagcgcgc cgctgactgg
     6901 caatagataa agtagccaag gatgtgctag tcggatatct gtcacgaacg tcaggctatt
     6961 taagaaaaaa gcagacaagc tccccatgaa tactccgaga atcgctgtga ccaccagcca
     7021 cttcaacatg tacatcaaaa tacgcactgg actcggtaat tgtttttcca taaaaaattc
     7081 ctcctcaaat aaaaaaacgc ctactaaaga aaggggctgg ccccagactt tagtaggcgt
     7141 cattagctat cttacgttag catttaaagg cgaacaccat cacctattca cttccattaa
     7201 aagatagcat atttttctgt aaaaaacaat aaaattatcg ttatttcttt acacgttcag
     7261 caaattcata gaaaggagcc aatttcgtta actgttgatt acattgatca ctatcatgac
     7321 aaatataatt cccctttttc gtatacgtgc catcagaacc ggctttcgtt gtagaaagaa
     7381 agagcgagac attcgatgtt ttctgacaga tcgcacagat tcccttatta atcgttggtg
     7441 ataaagtacc gctgatccct tttaattttc catcttcgta ataaaccatg aatttttttt
     7501 gcgtccctgg atcattccaa ccgatgtacg tatgtgcttg caagtcgatt tcattccaag
     7561 cgggttgttt gagttttttc acttttcgga ataatttctc gatttgtggt ttgctcggct
     7621 gcataaacgg ctggacgatt tgcttcagct cctccaatgc tcgttctgct tgcgtttttg
     7681 tcagttttac atctaaaact gtcgttaaaa acgtatcgat ttctgatggt tcgtaaccta
     7741 atacatcgcg aatcttttca gctgctagag cctgaaccgt ttgtaccgtt tttgagtcat
     7801 tgacagatga gtacgcattt aataactgct ctacttctct ttttataaaa aaatattgat
     7861 atggtttcat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     7921 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     7981 ataagatacg ttttgcatct tatcatcacc ccctttaatg ttcattcttc tatgtacatg
     8041 attcattcga taaataaaag taaaccgtat cttgagcaga tagtcaactg caaacagaaa
     8101 aaggactgtg acaaaagtcg tgtcaaagtc cctttctaca aacaaacggt gttccagaag
     8161 tagcttcttc aaaaaataag ccg
//
LOCUS       DADOZM010000116         7784 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.120, whole genome shotgun sequence.
ACCESSION   DADOZM010000116 DADOZM010000000
VERSION     DADOZM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.120"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(187..810)
                     /locus_tag="J8I47_002249"
     CDS             complement(187..810)
                     /locus_tag="J8I47_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323674.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(969..2366)
                     /locus_tag="J8I47_002250"
     CDS             complement(969..2366)
                     /locus_tag="J8I47_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6323675.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2398..3369)
                     /locus_tag="J8I47_002251"
     CDS             complement(2398..3369)
                     /locus_tag="J8I47_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323676.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3743..4168
                     /locus_tag="J8I47_002252"
     CDS             3743..4168
                     /locus_tag="J8I47_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323677.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4656..5357)
                     /locus_tag="J8I47_002253"
     CDS             complement(4656..5357)
                     /locus_tag="J8I47_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ6323678.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5373..5813)
                     /locus_tag="J8I47_002254"
     CDS             complement(5373..5813)
                     /locus_tag="J8I47_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAZ6323679.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5813..6388)
                     /locus_tag="J8I47_002255"
     CDS             complement(5813..6388)
                     /locus_tag="J8I47_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAZ6323680.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7236..7715)
                     /locus_tag="J8I47_002256"
                     /pseudo
     CDS             complement(7236..7715)
                     /locus_tag="J8I47_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2220 a         1620 c         1243 g         2701 t
ORIGIN      
        1 agctattttt atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc
       61 cactaaacgg tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga
      121 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      181 tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg taataattga
      241 ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg aaggacttgt
      301 tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt catttgtctt
      361 tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag aataagcggt
      421 gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca aaagcaaaac
      481 ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa gtacgtcatc
      541 gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca attcgccttt
      601 ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt cttttttgaa
      661 attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag caagatctgc
      721 tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac cgaataacca
      781 aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt aattttgact
      841 attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata
      901 attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt gctctcaact
      961 attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca atgacccgat
     1021 tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc atatcgattc
     1081 ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata gcctaaacaa
     1141 ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac aaattcgatc
     1201 cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt ttctacaccc
     1261 ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac attgatcaaa
     1321 atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat tttacgacca
     1381 ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt atcgattggt
     1441 gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag atctactgta
     1501 ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc aagttctttt
     1561 actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag gaatgaacga
     1621 ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga ttcgtctctt
     1681 ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc catttcatgg
     1741 taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg gatcgctttt
     1801 ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc gactggaacg
     1861 attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc aaaacaactt
     1921 ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc tttttcttgc
     1981 agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc gttttcaatc
     2041 aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt tccagattca
     2101 tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc agtctgtttc
     2161 atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc aggtcctact
     2221 tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt ctctcctttt
     2281 ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa
     2341 tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta gttcgtttta
     2401 gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt cctcttcttt
     2461 tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg ttggtggcgt
     2521 gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat agtgactgaa
     2581 cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg caccaatcga
     2641 catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg cttttgcccc
     2701 gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga tcgatggatc
     2761 attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt ctgtttgagt
     2821 ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga tcacttttgc
     2881 tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt ctggacaatt
     2941 caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc actcttcttc
     3001 ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa ttttttgttc
     3061 aattttttct ggctgatcgg caaacccgct taataataag ctatacccat tttcttcagc
     3121 tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc cgtttatcaa
     3181 aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt tttctttata
     3241 gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca gttgctgacc
     3301 attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca catcacggat
     3361 cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt gttgttcgat
     3421 tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat aactaaagtc
     3481 ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt gaaccggttc
     3541 atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag
     3601 tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt gtgggtgttt
     3661 tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata atttctttat
     3721 cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg
     3781 acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac
     3841 aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg tatatacgct
     3901 aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat
     3961 ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat ctgaagaaaa
     4021 aacagtgctg acagatatca agatcactac caaagatgac gaatatatta tttcaggaaa
     4081 agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc gtttgaagga
     4141 tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt tgtttctccc
     4201 cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg
     4261 atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgacc
     4321 aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca tttaaaacat
     4381 ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca aatccaagca
     4441 aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt
     4501 catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa agatttattt
     4561 atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct acatcacgta
     4621 gtaatttttc tttcccgtcg tttggttttt tatttttagc ctaattcttg accttttgca
     4681 tgctggttat ccgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct
     4741 tggatctgac ggatcacctt ttttgcatct tctggatttt ctacaaaatc gaatttatcc
     4801 ccatccactt gtattttagg acatacatca aaatcttcat accattgatt atagcgggtg
     4861 ttcaacgttt cataataggt atataacgtc tcatcgcttt ctagctgttc atagtcacgc
     4921 ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa
     4981 tcaggccgtt tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta
     5041 tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcgtttag atggaacaac
     5101 aggctatctt catagatgga tcggtctagc acattgtcat cgttcactaa agcatttttc
     5161 attgctaaaa atcgcttgtt caaaaagaag atttgcaata aaaaagcgta ctgctcagga
     5221 tttgaataaa agagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa
     5281 tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa
     5341 atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca
     5401 acaggcaatg tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtatgg
     5461 agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacaat gatcatcggt
     5521 tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc
     5581 tcaaagtcaa ggatagcaac aacaaattct gcatttgtca gttcttccat atctttttcg
     5641 tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt
     5701 ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact
     5761 cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg
     5821 cctccttcaa taataactga tttttaatag atactgctcg taattcattg accagttcca
     5881 atggattgtt ggaaaagatt acttgatcca accatttttt atccataaaa tcctcttttg
     5941 caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga
     6001 ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag aagttgtgag aattcctcca
     6061 atgtacctag acctccaggg aaaacgagga cagcatctcc cttttcaaat aataatacct
     6121 ttctttcatc gatcgtttct gtaggaataa atgtagtaac atcttttcca ggcaattcag
     6181 tttcaaaaag cccattcgga tagatacctg tgacttttcc gttattttcc agtactgttt
     6241 tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt
     6301 gtgccatgta ttttcctaac gtttgcgcaa taaatttgta actttcttta ttaccaaaac
     6361 gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct
     6421 tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt tagtggtata
     6481 taggatagac gccactatat atagtattta gaaaaaaatc atttttcagc cattttatag
     6541 ataaaacaaa aataaataac gtgttttcat cgatatgaaa tgacttactt atagaagata
     6601 ctgggatttc gtcctatatg ccaataacgt gtattatttt aaacaacaaa tacttgtttc
     6661 ttttattaac gaaaaaatca ttttgttctc gtgttatgtt tcactgataa atgatgtttc
     6721 tctaaataaa aaagccgacg aactaaacga tcaaaatttg atcatttttg ttcatcagct
     6781 tctcctaaaa atctttttgt taacgataaa tagctggatt tgtaaaaata tcacttgaat
     6841 cgtcactgct ggaagaaaat tcagtgataa ccgcacctgt tgcgcctgcc tggaactagt
     6901 gtttgatatt gggctcgaac gtgtactgat ctctagcatg aagaataatt tcttttttta
     6961 ctgtataata actttctttc ctttttggaa tagtacagga aagttttttt gtttcctttc
     7021 cttcgacata aggtatacca gaccctttcg acaacgaaag gttctcattt ttccttcatc
     7081 gtcatttcaa ggagggtgcc gatgctctgg acagtttcga tcgctcctaa accaaaatct
     7141 gtatattcga ttctttctaa ctctgattct ttcataccat tcctgtctca aggtacatct
     7201 attgtacttt tttcttgtaa tccattctgc ctctcctaac taattgtcaa atcatcccaa
     7261 gatatgtctt cttcctcttt ttcttcttgg atgatttctg cattcggatc tactggtttt
     7321 ttcaataata aaatggttat tcctgtgaca aaagaaccag ctaacaatgc accaatgaat
     7381 gcccatggtc tggtcatcgt agcgactgca aacattcccg atgccggaat acggctatct
     7441 gcgccccaaa acatgcttaa tccgccacct actgtcccac cgactcccgt tgctatgaca
     7501 ttgcgtgcca agtcgttcaa tacgataggg aaacatccct ctgtgatctg acagatcccc
     7561 ataggaaaag cggtcttcaa tgtatcgatt tcttgacgat tgagtatatt tttacgaatg
     7621 attttcccta agaaataagc gatcgtcaag ccgaatgggg ttaccatcga agctaaaatc
     7681 aatacagtga ttggttcata gattccttct gaaaatcgtg ccacaccccc ttatccagaa
     7741 taaatggtaa tccagaaaca gccgtgtatc tgcggtaata agcc
//
LOCUS       DADOZM010000117         7761 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.121, whole genome shotgun sequence.
ACCESSION   DADOZM010000117 DADOZM010000000
VERSION     DADOZM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.121"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            147..1094
                     /locus_tag="J8I47_002257"
     CDS             147..1094
                     /locus_tag="J8I47_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6323681.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="J8I47_002258"
     CDS             1106..2026
                     /locus_tag="J8I47_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6323682.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="J8I47_002259"
     CDS             2067..3524
                     /locus_tag="J8I47_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323683.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="J8I47_002260"
     CDS             3869..4483
                     /locus_tag="J8I47_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ6323684.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="J8I47_002261"
     CDS             4476..6200
                     /locus_tag="J8I47_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6323685.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="J8I47_002262"
     CDS             6197..7648
                     /locus_tag="J8I47_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323686.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2617 a         1291 c         1544 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgag ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DADOZM010000118         7751 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.122, whole genome shotgun sequence.
ACCESSION   DADOZM010000118 DADOZM010000000
VERSION     DADOZM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.122"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(13..348)
                     /locus_tag="J8I47_002263"
     CDS             complement(13..348)
                     /locus_tag="J8I47_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ6323687.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..604)
                     /locus_tag="J8I47_002264"
     CDS             complement(332..604)
                     /locus_tag="J8I47_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAZ6323688.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="J8I47_002265"
     CDS             complement(1055..1858)
                     /locus_tag="J8I47_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAZ6323689.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="J8I47_002266"
     CDS             complement(1836..2960)
                     /locus_tag="J8I47_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323690.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="J8I47_002267"
     CDS             complement(2976..3791)
                     /locus_tag="J8I47_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ6323691.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4662)
                     /locus_tag="J8I47_002268"
     CDS             complement(3781..4662)
                     /locus_tag="J8I47_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ6323692.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="J8I47_002269"
     CDS             complement(4738..6027)
                     /locus_tag="J8I47_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ6323693.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="J8I47_002270"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="J8I47_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(7376..>7751)
                     /locus_tag="J8I47_002271"
     CDS             complement(7376..>7751)
                     /locus_tag="J8I47_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6323694.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2365 a         1672 c         1192 g         2522 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacagcc agactgtaga caaaagctgt tccaaactcg
     7441 taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa caaaaaaata
     7501 aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt gcagcaaaca
     7561 ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa cgcatcccat
     7621 gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt ttgtaaattg
     7681 atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct ccccaaacat
     7741 gtctcgtgat g
//
LOCUS       DADOZM010000119         7743 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.123, whole genome shotgun sequence.
ACCESSION   DADOZM010000119 DADOZM010000000
VERSION     DADOZM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.123"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..906
                     /locus_tag="J8I47_002272"
     CDS             <1..906
                     /locus_tag="J8I47_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ6323695.1"
                     /translation="LKPEDYPKIFPNRVFDFQLDQTGQTELQVGIQAVAKNIGIQIRD
                     MTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSE
                     LDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIATWTKNSQTLDDKEAAERGKIMNDV
                     SRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKD
                     GKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSL
                     ETVSEVAKRKNEIYYEQQLSQKERV"
     gene            967..2427
                     /locus_tag="J8I47_002273"
     CDS             967..2427
                     /locus_tag="J8I47_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAZ6323696.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            2438..4642
                     /gene="topB"
                     /locus_tag="J8I47_002274"
     CDS             2438..4642
                     /gene="topB"
                     /locus_tag="J8I47_002274"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ6323697.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            4888..5481
                     /locus_tag="J8I47_002275"
     CDS             4888..5481
                     /locus_tag="J8I47_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6323698.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            5497..6396
                     /locus_tag="J8I47_002276"
     CDS             5497..6396
                     /locus_tag="J8I47_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAZ6323699.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            6399..6884
                     /gene="ssb"
                     /locus_tag="J8I47_002277"
     CDS             6399..6884
                     /gene="ssb"
                     /locus_tag="J8I47_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ6323700.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            7250..7510
                     /locus_tag="J8I47_002278"
     CDS             7250..7510
                     /locus_tag="J8I47_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323701.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2831 a         1337 c         1609 g         1966 t
ORIGIN      
        1 ttgaaacctg aagactatcc gaaaatcttc ccgaaccgcg tctttgattt tcaattggat
       61 caaacagggc agacagaact tcaagttggg attcaagcgg tagccaaaaa tattggaatt
      121 caaattcgag atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg
      181 gcgtacgttc gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact
      241 cctacgcagc atttagccac aagtattcac gaattagccc atgctagaat gcacaaattt
      301 tcagaattag atactgcaac aaaagaacta caggcagaaa tgacctccta tattgtgtgc
      361 aagcattttg ggatggacac ctcggagaaa gctattcctt atattgcgac atggacgaag
      421 aatagtcaga cgttggatga taaggaagct gcggaacgtg ggaaaatcat gaatgatgtc
      481 agtcgtgtcg caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa
      541 agagaaccag agatccagcc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg
      601 gcagaacaaa agaatattgt gcccgttggc tcactttgga tcgacaagaa agacgggaaa
      661 gtcctggaag aagacacggg ccgaacgtta catttgaagg atgcggcgtt tattgaagat
      721 ccgcagcata atcaagtgat tttaaaagga aaaagttatg gaaaagagat ccaaacggcg
      781 ttaccttcag aagaatttgc gctaggaaat ccattccgat tttcacttga gacagtgtca
      841 gaagtggcaa aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc
      901 gtataaaaca agcaagaaag actatctgaa aaatggatag tcttttttct tttgggaggt
      961 ttgaaaatga cagagaaaaa aatgagtctc attgatcgat gcaagcaaat ggatattgtg
     1021 gattttgcac gaaacaatgg tttagcggtg gccaataaag gacgagacta tcgcttagaa
     1081 gaccatgatt catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat
     1141 attagtggcg acattattga attagctaaa ctttttttca tagacaaaga gatccaagac
     1201 cctaagcaac aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa
     1261 cgagtggaaa atcttcattt tgagacagaa aagtacaaag atcaccccgt tgattatcaa
     1321 ccactcacag aaaaaggaag gaattatctg aaggaagaac gaaaactgcc cgattggttg
     1381 attgattatg cagaaaaaga aggattgatt gccgaactaa agccaaaaca tgaacggcaa
     1441 aatttcttag ttcgagacga tcgtttggat catgcggtcg cctttttatg gaaagatcca
     1501 cagacaaaag agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt
     1561 ggtgagagag gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac
     1621 ctaaagattg gtgatcctaa acagttaaaa ttttttgaga gtagtattga tttacttagc
     1681 tacgctgcac tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta
     1741 aaacaccatg tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa
     1801 gcctttcctc aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac
     1861 gaaaaagaac aattaatggg cgcagttgat ccatttacga atcagaaagt acgttgtgaa
     1921 cgaggaatcg ctaatgattg gcaagtgcca agagaataca aagtcattta tgaagaagtc
     1981 gcaaaagaag aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat
     2041 ctgcaattaa cgaaccaact ggtttccgct cacaaagtca atgcgtcttt tggtcagcag
     2101 ctttctgtta acgattcaat agaagtcatt agtttgaaag atatttgtag agaagtcgca
     2161 aaagaattaa aagattgtga acgctcgaat ggtacgtatg attttgatcg tttttatcaa
     2221 ggaaaagggg atattaattc tcagattctt ttctcttata aagcagaaca atactacaaa
     2281 ggctataaaa atcatgaaca tgaatttgta cctgaagtga aaaaggattg gaacgatcaa
     2341 ttgaagcatg agatccatca acaggaaatt agaaagcaaa aacgtgcgat gttgtttcaa
     2401 caaggcagac aacaagaaag ggagtgagat cgtcacgatg aaaactgtca ttttagcaga
     2461 aaaaccgtca caagcgaagg cctatgccga tagcttttct aaagccaccc gaaaagacgg
     2521 gtattttgaa atccaagatc gattgttttc gggtgaaact gtgatcacgt atggttttgg
     2581 tcatttagtc gagttagatt cgcccgatat gtacgatgaa aattggaaac aatggtcatt
     2641 agagcatttg cctattttcc ctacacatta tcactatcat gttcctaaag ataagaaaaa
     2701 gcaattcaac gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga
     2761 tagcgatcgt gaaggggaac tgatcgcttg gacgatcatt caacaagcag gcgctgatca
     2821 aggaaaaacc tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg
     2881 attccagcaa ttacgagacg cagaagagac ctatcccaaa tttgaagaag cacaagcacg
     2941 ccagatcgct gattggttga tcggcatgaa cggcagccca ctgtatagtt tactcttgca
     3001 gcaaaagggc ataccaggaa gtttttcctt aggcagagtc caaaccccaa cgctttacat
     3061 gatttatcag ctgcaggaaa agattagaaa cttccaaaaa gagccttatt ttgaagggaa
     3121 agcccaagta accgctcaaa atggcatatt tgatgcgaag cttgatccca atgaaaccca
     3181 agcgacgcaa gaagcatttg aagcctattt aaaagaaaaa ggtgttcaag taggaaagca
     3241 gccaggcacg attcttgaag tcgaaaccga gaaaaaaagc gcggcaagtc cacgtctttt
     3301 ttctctatcc agccttcagt ctaaaatgaa tcagctcatg aaagctagtg ccaaagatac
     3361 tttggaagcc atgcaagggt tgtatgaagg gaaatatcta agctatccac gaacagacac
     3421 cccttatatt acagaaggag agcatgcgta tctgttggat catttagatg aatacaagca
     3481 ttttttaaag gctgaatcat tccctactcc tattcataca ccgaatagtc ggtatgtgaa
     3541 taataagaaa gtccaggaac attacgcgat cattcctaca aaaacagtta tgacagaagc
     3601 aacgtatgaa aagctgacac ctttacaaca agcgatttat gatcaagttt taaagacgac
     3661 ggtggccatg tttgcggaaa aatataccta tgaagaaact acgattttga cacaagtcca
     3721 gcagcttcag ctaaaggcag tcggaaaagt accagtagat ctaggttgga aaaagctatt
     3781 tggcaaagag agcgacagaa aagagaagga agaagaaccg ctacttccta aagtaacaaa
     3841 aggagaaacg gtgacggtag atctacaaat ctttgaaaaa gaaaccaagc caccacaacc
     3901 ttacacggaa ggtacactaa tcaccgccat gaagacagct ggtaaaaccg tggatagtga
     3961 agaagcacaa tccatattga aagaagtgga gggaattggt acagaagcga cacgagcaaa
     4021 tattattgaa accttgaagc aaaaggaata tatcaaggta gagaaaaata aacttgtggt
     4081 gacgaataaa ggaattttgc tttgtcaggc tgttgaaaaa gaaccgctgt taacaagtgc
     4141 cgaaatgacc gccaaatggg aaagctatct tacaaagatt ggtgagcaaa aagggacaca
     4201 agctactttt ctagcaaata tccaaaaatt tgtttcccac ttgttggaag tcgtcccagg
     4261 acaaatccaa gccactgatt ttggagcaac gttacaagag gtgaaagtag ccagtgaaaa
     4321 gcaagaagcg gcgcgtcatt taggatcttg tccaaagtgc caggaaaaag aagtcatact
     4381 ttatcagaag gtggcgactt gcacgtctga agcctgtgat tttaaattgt gggtaacgat
     4441 tgccaaaaag aaactcacag cgacacaact gaaagagatc attcagaacg gccgaacaag
     4501 tcaaccagtc agaggactaa aaggtcagaa aggttcgttt gaagcaacga tcgttttaaa
     4561 agaagacttt acaacaggat ttgagttttc tgaaaagaag aagaccacct acaaaagaaa
     4621 agtgagacgt aaaacgagtt agaattgacg gagggaaaaa cgatgagaaa tacaacgcat
     4681 aaagaacaag aaatcgaaaa tcaaaaagcc aaaatacgtt cagagcagga gaaaatgcgt
     4741 gccagcaaac gaagactaca gcaattgaag aatcaacaac ggattcaaaa agaacttgaa
     4801 ttagatcgat aaatcctcct gttgttaata ttaaactaac atgttatagt tatgttagtt
     4861 taatattaac attcgagagg gaaagcgatg aaagataaag tcaagtgcat ttttgagaaa
     4921 tacaatggta atctatcctt gaaggatgct agaaatgaag ggttatcacc tatgacgtta
     4981 gatcgtttag ccgaaaagga caaaatagaa aaaatagcat caggttttta taccttaaaa
     5041 gatagttttg tggatgaatt atatctcgct caaagtatct atactagagg aatcttctct
     5101 cacgagacag cgcttgatct ttttcaagta ggaacatata tccctaaaaa aattaatatg
     5161 atgtttccta aaggatacaa tgtttcgaaa gagaatttag atcagtatgc cattcaaccg
     5221 cattacacga ataaagaaaa ctttgaactg ggaatcacag aggcagaaag cttttacggc
     5281 aataaaattt tcgtctatga tttagagcga acgttatgtg atatgtggaa tcccagatat
     5341 aaagcaagcg tagaagtaaa acaagaagca ttaaaagaat atatgacaag taccagcaga
     5401 aatacaaata agctaagaag atacatgaat attttaaaat caccaaaaga aatgactttg
     5461 tatatgttgc cactttattg agaggagtaa aggtgaatga caccgacaca atttaaagcg
     5521 aaaacaagaa atgttgcagc tgagaaacat ttaaacccac aaattgttca aagatattat
     5581 atgatggaaa aattattaga atgtattgtt gaatcgcctt atcatgatga ttttgtatta
     5641 aaagggggat ttctgatcgg atcaaaatac gggttagaaa accgttcaac cgttgatatt
     5701 gatacgactt atcgaaattc gaaactaaca aaagaaaaat tagaaactgt tttttctgaa
     5761 ttaacagctg ctcctacaaa agatggcatt cggtttgaat taaagggctt aacagagacg
     5821 agggaagccg attattaccc aggttttcaa gcaaagttta ttgcttttct tgaaaatgca
     5881 cgtgtacctt ttaaattaga tattacttca ggtgattcta ttattccagc agcattgata
     5941 tatgaacata aattgatgtt tgaagataaa acaattcata ttccagctta tcctacggaa
     6001 caaattttag ctgaaaaatt acatgccacg tttagtttcg gaatagataa ttcacgtatg
     6061 aaagatttct atgatctgta tatgatccct aaactagaac gtatcgatga gataaatttg
     6121 tataagagtg ttcaaggaac atttaaggaa agaaataatc aatcagtgtt taagaatttg
     6181 tataatcagg aaatgcctca attgatgaag aatgagggat tgagggagaa atggaaaaga
     6241 tatcagcaag aaaactattt tgccaaagat atttctttcg aatctacctt aacttctgtt
     6301 gattcgctga tgcaatcaat tgtcgagcaa gaggaagtag aacgaaaaca aaattttaag
     6361 agatcacaac aaatgaatca agaaatggag agataaagat gatcaataat gtaactttag
     6421 taggtcgatt aaccaaagat cccgatttac gttacaccgc aagtggcact gcagtgacca
     6481 cttttacctt agccgttaac cgtaatttta ctaatcaaag tggcaatcga gaagctgact
     6541 ttatcaattg tgtgatttgg cgcaaaccag ctgaaacgat agcgacatta gctaaaaaag
     6601 gcagcttgat cggcgtcgtc ggacggattc aaacgcggac ttatgacaac cagcaaggac
     6661 agcgtgtcta tgtgactgaa gttgtggccg ataatttcca actattagaa agtaaagcag
     6721 cgacagaaag ccgtatgcac gctgatcaat cgtccagcag tccaagccct accacctttg
     6781 aacaacggga tacagcgacg cctaacaaca atggattgaa cgcttcacag aatccatttg
     6841 gtggacaatc aatcgatatt agcgatgatg atctaccgtt ctaagtagca ttttctattg
     6901 gttgcatttg ctacggttta tgttataatt tagacaacaa aaagaatttt caatctataa
     6961 ccaaaaacca ctagctggcg ggctagtggc tttcttttac gtttctatta taagaattaa
     7021 cgggtaggct tactgtcttt cttggcaaat ttacaatcaa gccatttgca gatatagtgc
     7081 gcggctactt ctgccatgat tgcaaaaaca aaatcaaaaa gaattttcaa acctataacc
     7141 tcctcccctt gccagattag gccggggtag cagacagcaa gactattata gcataattga
     7201 aatatccaaa tgattggatt tttgctataa ttttagcgag gtgattgtga tgaacaaata
     7261 tgttggtttg cttgataaaa tccgagtaat aaaaacgcaa ccgttattgg ttcgctttac
     7321 tttacaaacg attcatgaat cgattaattg tgtagttgca gatattgaaa ttatcgataa
     7381 attgctaatt atggatgatg gaaaatacaa tattgccgtc acaggtcact tcaataaacg
     7441 aaatcagcta gttattgcta gcatgtatgt tagaaatccc gatcatttta cgcgatcgat
     7501 gggcatataa ataaaagaag agttgtagtc ctaaatgagg attgcagctc tttttgtttt
     7561 acatagaaac aggaaaagac ctttttagga agacacaagt gccttctcaa ttgaaaaacc
     7621 tttctctcac tccaaccgcc accgccgttc gagaagaaag gaagtgaacg ctgcacgttc
     7681 actaagatat ggtgtcttgc aaagcaagac aaaatagaca aaacttcctc tcaccaaaag
     7741 gtt
//
LOCUS       DADOZM010000120         7680 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.124, whole genome shotgun sequence.
ACCESSION   DADOZM010000120 DADOZM010000000
VERSION     DADOZM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.124"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..235
                     /locus_tag="J8I47_002279"
     CDS             <1..235
                     /locus_tag="J8I47_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323702.1"
                     /translation="FLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLE
                     YWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(361..1038)
                     /gene="nth"
                     /locus_tag="J8I47_002280"
     CDS             complement(361..1038)
                     /gene="nth"
                     /locus_tag="J8I47_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAZ6323703.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1045..1743)
                     /locus_tag="J8I47_002281"
     CDS             complement(1045..1743)
                     /locus_tag="J8I47_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAZ6323704.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2011..3195
                     /locus_tag="J8I47_002282"
     CDS             2011..3195
                     /locus_tag="J8I47_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAZ6323705.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3201..3644)
                     /locus_tag="J8I47_002283"
     CDS             complement(3201..3644)
                     /locus_tag="J8I47_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6323706.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3870..4157)
                     /locus_tag="J8I47_002284"
     CDS             complement(3870..4157)
                     /locus_tag="J8I47_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323707.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4328..5680)
                     /locus_tag="J8I47_002285"
     CDS             complement(4328..5680)
                     /locus_tag="J8I47_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAZ6323708.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5953..6399)
                     /locus_tag="J8I47_002286"
     CDS             complement(5953..6399)
                     /locus_tag="J8I47_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ6323709.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6635..7561)
                     /gene="cysK"
                     /locus_tag="J8I47_002287"
     CDS             complement(6635..7561)
                     /gene="cysK"
                     /locus_tag="J8I47_002287"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAZ6323710.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2075 a         1566 c         1255 g         2784 t
ORIGIN      
        1 ttttctgatg agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat
       61 ttttttatta tcacttatca tttttattac acctttagat agatacgtca cttatttata
      121 taatcctttg gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat
      181 cgggtatttt cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt
      241 taccatccta gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac
      301 agcttttctc cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt
      361 ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc
      421 ttgacatatt gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata
      481 tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg
      541 catcagtgtt tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt
      601 tgagacacgt tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa
      661 tacgacattt gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt
      721 tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc
      781 tttatttcga tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc
      841 tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga
      901 tacatctgtc gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg
      961 cttgagttct ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt
     1021 tttttgtttg cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg
     1081 caattcttct tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg
     1141 tttttggtct tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa
     1201 aatgcggtca acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag
     1261 cttcaaaatt tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc
     1321 gatcgaagaa agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc
     1381 tttttcttgt gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag
     1441 atcatagatg gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc
     1501 tatcttgata aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt
     1561 gattcccata tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc
     1621 catatgcaac tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt
     1681 tttaagtaaa agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa
     1741 catgatttct cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag
     1801 gtttcttttc tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt
     1861 gtttgttcat gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt
     1921 cgctgttcct tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa
     1981 gttaacatta ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa
     2041 gtttttggat acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta
     2101 cttccgtttt taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact
     2161 ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta
     2221 agtgatcatt ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga
     2281 tatttgcttt ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa
     2341 ggattgacgg caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct
     2401 aatgaaagaa ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg
     2461 ggtcctatta tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga
     2521 gcactcttta ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca
     2581 ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa
     2641 caattattca agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg
     2701 gcagctggct ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag
     2761 gctgggttgg ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg
     2821 ttcattatgc ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg
     2881 ttcagtgaaa tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt
     2941 ttgtttttat ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc
     3001 aatggccaac tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc
     3061 caaagtatac aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct
     3121 gcagcaccat ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac
     3181 tacaataaaa aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata
     3241 aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc
     3301 tgtacccatt tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc
     3361 aattgaaaat agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta
     3421 gctatcagtt tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct
     3481 aattttgtca cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt
     3541 agagtaaatc ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta
     3601 gctaaatcca gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt
     3661 tctttcatta tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa
     3721 aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc ttgtcataac ccctttttct
     3781 gattaaaaga tagtaaagag acagtcatcg gtaattgtga tgattttttg tcatcactta
     3841 aggatgagcg gccttttcaa aaactgtctt taaagatcat catcagtttc tagcacatat
     3901 ggctgacctt catatgtgta ctctgtattc aatgtgccgt cattttcatc tgagtagatg
     3961 cgtctactga aaggaaaacg atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg
     4021 taatattcct cttgcgtttc attcaactga gagagtactt gatctgccaa ctcatcaata
     4081 ttgatcgtta atttcttcga agcactttta taatcttcta aaaatgcatc ttgttggaca
     4141 aaatattctt tattcatctt ctttctcacc tttcttttca aaatcaaata aagctgcttg
     4201 ttcttttatg taacagcttt atctttgctg cttattgtga caaaaaattc gaagaaagac
     4261 aactaattgt acttttcaca aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc
     4321 tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt
     4381 aattccatgg gcggtcaaaa tgtggttgga agaagaaatc tgaaagtgcg agatcttcta
     4441 cagtcatttt attttgaaca gctaaagaca tggtatttgc tgattgtgta atgtcgtatt
     4501 tggacatgaa ctgagcacct acgatacgat tcgttccttt ttcatagact agctccatca
     4561 ttactttttc agtagttggc atgaactctg gacgataatt atcttcaaat acagtcgctt
     4621 ggacttctag atcgttcaaa gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc
     4681 agccaaatag gtaaagtcct gaagttcctt gagtaccacg atacgccatt ttatgatctg
     4741 tcaagttatt tcccacaagc aaaccttgac gaacggcatt tgttgccaaa ggaatatagt
     4801 tttgcgtgcc acttggattg tagttgacta ctgcagagtc tccagctgca aaaatgtctg
     4861 gattacttgt ctgcatgtat tcattcactt tgattgcccc gtttggcagt gtatctactt
     4921 ttccttcgat cagttttgta tttggacgga agcccacaca taaaatgacc atatctgtat
     4981 caaatgtatc tgttggtgta gctacttgtt tcacttttcc ttcttcatct gtgataaatt
     5041 cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc tgtcaattct ttttctaaga
     5101 tatctgtgaa tggtttatct agatatttat tcaagatgcg atctaatcca tcaatcaatg
     5161 tcacttgttt tccagattcg acaaaagctt ctactaattc aataccgata tatccaccac
     5221 caacaattac tacacgtttt gcttctttcg cttgtgcaat aatttcattt gcttgattgt
     5281 agtttttgca taggagtaca tttttcgcat cgattccact aatcggtggg ataataggcc
     5341 atgaaccagt tgtcatcact aatttatcat aagaaaccgt ttctgtttct cctgttttca
     5401 gatcttttgc cgttactgtt ttattttctg tatcgacgtc tgttacatcg tgttccattt
     5461 taactgttgc tcctaatgaa gataattctt caggatttga gtagaacaag cctgctgggt
     5521 cctttactac tccgccgaca taaagtgcga taccgcaaga taagaaagaa atattgtcat
     5581 ttctttcata tactgtcact tctgcttctg gatgattttt taaaatactt tttacagctg
     5641 cagtacctgc atgagtacag ccaattacta ctactttcat ttatggtccc tccgattaac
     5701 attttgtcct tcgacaaaac tactataaca gaaataaaac ggatacaaca gtttaatgct
     5761 tgtgaattca caaaggtttg tttgtagttt cattcctttt caagcataag aacccaatat
     5821 ctacaagatt cttattttaa taatattcac ttttatatct aagattgtga ataaaagaca
     5881 aaaaaataaa aaagctagcg ttttttttac acagctagct ttttttaggt ataacacttg
     5941 tagaaactgt ttttacattt gcgccttttt tgctgactct ttacaatccg gacagagtcc
     6001 atacaattcc aaccgatgtt cattaatctc aaaatcagtc agtctgcttg cagccatttc
     6061 tatatcttca agccctgggt aatgaaaatc aacaatctta ccgcagtttt cacaaattac
     6121 atgataatga cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc
     6181 ttgaacaaat ccaatcgaag taaataagcg aaggttattg taaacagtcg caacactcat
     6241 atttggaaac cgatgttcaa gtgctttata aatctcatca gccgttggat gggaatgatg
     6301 ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt
     6361 taacttttcg ataacctctt cgactaactt attttccatg taaacacccc acgttgttat
     6421 tctcgtctta tcatactatt tttacatttt aaaatccact taacatgaaa agaaaattca
     6481 attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat tctacgtctc
     6541 tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt taaaagtttt
     6601 ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag
     6661 ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt
     6721 cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc
     6781 cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga
     6841 ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga
     6901 ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga
     6961 ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc
     7021 ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct
     7081 caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta
     7141 aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat
     7201 ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga
     7261 tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg
     7321 acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta
     7381 gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc
     7441 ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat
     7501 ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca
     7561 tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat
     7621 ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtaatt
//
LOCUS       DADOZM010000121         7649 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.125, whole genome shotgun sequence.
ACCESSION   DADOZM010000121 DADOZM010000000
VERSION     DADOZM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.125"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..179)
                     /locus_tag="J8I47_002288"
     CDS             complement(<1..179)
                     /locus_tag="J8I47_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAZ6323711.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="J8I47_002289"
     CDS             complement(192..1295)
                     /locus_tag="J8I47_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAZ6323712.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="J8I47_002290"
     CDS             complement(1385..2320)
                     /locus_tag="J8I47_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAZ6323713.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="J8I47_002291"
     CDS             2622..4556
                     /locus_tag="J8I47_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ6323714.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="J8I47_002292"
     CDS             5013..5429
                     /locus_tag="J8I47_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAZ6323715.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="J8I47_002293"
     CDS             5443..6009
                     /locus_tag="J8I47_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAZ6323716.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="J8I47_002294"
     CDS             6032..7018
                     /locus_tag="J8I47_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAZ6323717.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="J8I47_002295"
     CDS             complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="J8I47_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAZ6323718.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2192 a         1466 c         1578 g         2413 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DADOZM010000122         7579 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.126, whole genome shotgun sequence.
ACCESSION   DADOZM010000122 DADOZM010000000
VERSION     DADOZM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.126"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..111)
                     /locus_tag="J8I47_002296"
     CDS             complement(<1..111)
                     /locus_tag="J8I47_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ6323719.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVH"
     gene            complement(122..1498)
                     /locus_tag="J8I47_002297"
     CDS             complement(122..1498)
                     /locus_tag="J8I47_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6323720.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(1627..2274)
                     /locus_tag="J8I47_002298"
     CDS             complement(1627..2274)
                     /locus_tag="J8I47_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAZ6323721.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2358..3785)
                     /gene="uxaC"
                     /locus_tag="J8I47_002299"
     CDS             complement(2358..3785)
                     /gene="uxaC"
                     /locus_tag="J8I47_002299"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAZ6323722.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(3859..4938)
                     /gene="uxuA"
                     /locus_tag="J8I47_002300"
     CDS             complement(3859..4938)
                     /gene="uxuA"
                     /locus_tag="J8I47_002300"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ6323723.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4941..6572)
                     /locus_tag="J8I47_002301"
     CDS             complement(4941..6572)
                     /locus_tag="J8I47_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAZ6323724.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6889..7560
                     /locus_tag="J8I47_002302"
     CDS             6889..7560
                     /locus_tag="J8I47_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323725.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         2455 a         1512 c         1035 g         2577 t
ORIGIN      
        1 atgaacatca aaaatctctg cagcaattgg cccgaaaaag agattgtgtt tataagtcat
       61 tgtatccatg acacaaccat ctgaatcaat acaaaatatc caactgttca tgttttttct
      121 cctagttatg tttttgattt agcaaattat aattttctaa tactttttct ttagataatg
      181 gatatacgaa aaataaaatc aatcctacaa caaagaaaca aactgctgga atcaacgttg
      241 aagagttata cagcttttgt aaaacttctc cactttgtac ttttgctaac gaatcatacc
      301 ctattataga aagagtccaa cctccgacac cgccagctaa agcttgtcca atttttcggg
      361 caaaagaata tactgcataa atagttccat cttctctttg gccagatttc acttcttgat
      421 catcgattac atcagtaata ttcgcccaaa tgactgtatt aaaaatacct aaccccatat
      481 atccaacaga agtaagaact aagaatacat acatattact aggctttatc aaaaataaaa
      541 gtaaatatac aatagcagag aagaacatcc ctactgacgc caattctttt ttaccgtatt
      601 ttttactgaa aattaaactt acaggtgaaa caagtaccaa catcaaaata ggattgataa
      661 agttcattaa aactattccc tgtgcattat tataataatc tggaaataca taattgttca
      721 tactcattgt taaaagctga gcaagcaaca aaaaaatagc tgcagctatt atagaaagca
      781 acgctctatt tttagctaat ccttttaaag atttcaacaa tgaaactttt aactgcgatt
      841 catcactttt aacccgttct acagttaatt tataacatat tacgtaacaa ataactgcac
      901 acactgaaaa tactcctgcc acaattgtaa aagttgatcc accacgtaca atttgagctc
      961 cagaactatc tcttgtataa ataaaaattg gtgttaacac gccaatgaac atacctgcta
     1021 aggtagctcc tatactacga aatgttgata acgctgtcct ttcttctgca acagaagtga
     1081 tagctgatgc cattgaaccg taaggaatat taatagatgt ataaaaaata cttccccata
     1141 gtatatatgt tacatacata tatataactt taatactcat agaagctgat gccattgatg
     1201 tttgatacat taaaaaactt gctaaagcga ctggaccact catccgtaaa atccacggtt
     1261 taaatttgcc agttttagat ggttttgatc catccacaat acgccccatt gttatgtctg
     1321 taactgcatc aataaatctt gctactaaaa ataatgttcc tacggtagct ccgcttatcc
     1381 ccaatacttt tgtataaaaa atcattaaaa aagaactagc aaaaatgaaa gtgaaatcat
     1441 tcccaaaatc tccaaacata tatcctattt tgtctcttag accaaatttt ctttccatat
     1501 aatttgcgct ccttcctatt gttaagcgtt ttcattctgt tgaactgcca acttacgtac
     1561 tatactctga agtataaaga gttagtattt attttttaca ttttatagta ttatttattt
     1621 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctattcgagt
     1681 tacttcttca aaatcacctt tttcaacagg agccaacaaa ttcccaccta caccaactgc
     1741 aacaacgcct gcttcaaacc actcttctaa gttatctaga cttactccgc cagtaggcat
     1801 tatatttaca tatggtaatg gagcttgata cgtttttaca acagatggac caaaagcaga
     1861 acctggaaat agtttaatga tttctactcc agcggtaagt gcctccttta tctcagtaat
     1921 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctctg ctgtttgtcg
     1981 atcaaaagat ggacttacga tatattctgc tccagccata attgcaatac gagcagtaaa
     2041 cgcatctaaa actgtaccgg ctccaattat tacatctttt tgatcagcat attttttagc
     2101 aatttctttg attatttctt cagcattcgg aactgtaaaa gttagttcaa tccctttaat
     2161 tccacccgca gtaattgcat ttattgcttt aatggcctca tctttatttt ttccacgtac
     2221 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
     2281 ctccttcttt tatggataca tactctagct agttgtacta acttacaata aataaattta
     2341 agcttttatt ttttgtatta ttttctactt tcgatttcaa aaaaattaaa ataattatac
     2401 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
     2461 cgacctgctt taacccactc gcttagtaat tgacataata ctcgacggaa atactcatgg
     2521 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
     2581 ctttgttgcg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
     2641 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
     2701 gctagtggaa gccaatcggt tggatttaat gaatataaaa ttgtttttgg taagcaccca
     2761 ctcttttcag cagtgtcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
     2821 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
     2881 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
     2941 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
     3001 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
     3061 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aataatccac tcgttggctc
     3121 aatgcctgct tcaacccatt aaagtttttg attggtatac ccgctgcttg actaagtttt
     3181 aaaatatagt tcgaaaatcc tgatgatttg atactccaga ccttatctgg acggaaagta
     3241 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatatcg caagtcatct
     3301 gtaggatcat ccgttgtaca aatggctttc acatccatca tcttcagcaa ttcttttggg
     3361 cgataggaat ccgtttggat tttttcgttg gcacgtcgcc aaatttctgg tgcattcttc
     3421 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
     3481 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
     3541 ttacctgcac cactgatcaa ctcttcttct atcccattag ctcgcatcaa tcgccattta
     3601 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
     3661 ttttctagca aacatgctgg gtctaaatga caatgataat cgatgattgg ttggtgttct
     3721 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
     3781 aacatattta agcactcctt tccttccctt agtttttaga agagaaaatt tttcttctaa
     3841 atagactcca ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
     3901 taaataaact gcacctagag ctcggtcata taacccataa ccaggtctac cggtttctcc
     3961 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
     4021 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcataaaa
     4081 atcgagtcca ttatcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
     4141 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
     4201 tccagtacat aacgtaatac cattagaagg agaatccact acattacaca accattctaa
     4261 atcgtcacga tttttaacaa tacgaggtaa tccgaaaata gaatatggtg gatcatctgg
     4321 atgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
     4381 tgcaaagttc tctcgcaatt tcgcttcatc aacttcttta taggcttcta ataattgctt
     4441 gatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
     4501 tacttttcta ataatttcct ctggatcagt aggaatattt tttgaaatat aggctaacgt
     4561 agaagaccca tcatctaaat gataatccaa atctgatttt acccaatcaa agactggcat
     4621 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
     4681 attctcgatg tattgatctc gtgttggcaa accaattttg atatcatcat gaacattaac
     4741 actctcaata actttcaagg taaggccatt ggcttcgact tcatctttta aagctttaat
     4801 tcgttctctc ggccagacct ctccggctgg tatatccatt aatgttccaa caattccttt
     4861 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
     4921 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
     4981 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcaat tttctttcct
     5041 atctcaactt gatataaatc taaaccaaat atttccttat tttctaagat ttcctgtacc
     5101 gcacaatgaa tctttgcagg atcttgttca cctaatttca atactgagaa tttttcctgc
     5161 aactctacca ataaaggatc aggacttggc acaaaatttt ttccttgatc atcgactgcc
     5221 aataaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
     5281 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
     5341 gtatctgttg caattcgttg cggtgtatct ggaatatagg gattaggcaa acgtttattg
     5401 attacttcat caataaattg ttttggatca ataacgcctg gactttcaac tactggtaag
     5461 ccttctacat aacctatccc tttaattaag ccaaccaaat catcatcttg catttctgcc
     5521 tgaattgatt tataccccaa caaacaacct gttattgcta atgccgtatg gagcggattt
     5581 aaacatgtcg taaccttcat gatatctgct ttatcaactg tttctctatc tgtcataatc
     5641 acaccagccg cttccagttt aggtcgacca tttggaaaac tatcttcaat gactagataa
     5701 tggacttttt cagtattggt aaaagcagcg atattggtat ttttcgcagt atgaattata
     5761 tctaagtctt cgaaacccgc cttcaataaa tccattttga cactttccga aggatttggc
     5821 gtaattctat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
     5881 ataaattttt cagaaacgta tccttttttt tgccaagctt ctgcaatcat aaaaatgcta
     5941 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggtaac
     6001 tctccagcat taaacctgct atacaacaag gatgtcacaa tactcatcgt atgttgagga
     6061 ttggtaggtc cagattttat atctgcttcc actaccgaaa aaaattgtcc tttagaatct
     6121 tttaaactgt atcctttttc agtaatagta aacgttgtaa actgtaatgt aggatcttca
     6181 aaatatttaa ccactctttg atagtccgtt atattctttg gattgcaata taaactatca
     6241 gctgttactg ctaaaagtct ttttttgagt tcaccatctt cacccataac aatctccaaa
     6301 atatcattgt tgtagtcatg ataagctttt tctactactt cttcatcaaa tgtctcacac
     6361 acaactactc cggattctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
     6421 aatcctcgat aaagatttcc tccaccaaaa tgaagccaca ctggatgttt ctgaccttct
     6481 tgtttgaccg cttcgacatt aaaatacggt agttgaactt gcattttgtt caatccttcg
     6541 atagaatctt gccagtcttt aatcaaaacc atatttaccc ctccatactg atatgttagt
     6601 ttccgttaca attgtagtat aaaatcgaat gtaatcgctg tcaataagaa aaaactaaaa
     6661 atattctttt ttctaccatg tcagaattat tgtgtctttt tttagtaaaa gcacaccaat
     6721 atatattttt tagaactaac ttaaattaaa gcttaatttc tctttgatct tatttgagta
     6781 ttttttgttt ttttaatagc tagtatgtta ctttgaaatt tgatattata taacgagcat
     6841 agaactaaaa tatcaaattc aaaaatgaaa ttttgagagg gattaaatat gacaattaaa
     6901 actagtctcc aaaatcaagc ttatcaatct attcgtcagc aaattatcta cgctgactta
     6961 gaacctggaa aaaaagtatc agaaaaaaaa ttggaagaaa cgctttctat cggtcgtact
     7021 ccaatacgtg aagcattgat tcaactccgt catcaaggac ttgttgatac aattcctcaa
     7081 agtggaaatt atatctcatt aatcgactta gatttagcaa aaaatgctcg ttttgtcaga
     7141 gaacatttag aacgacaaat tatgctagag tgttgtgcca agatgaataa tcaaaagaaa
     7201 caagttttag aaactataat tgcagaacaa gaaaaagctg ttagtcaaaa gaatgctcgt
     7261 agtttttttc ataatgataa tttatttcat aaagcttgtt ttgaaattgc agaacgggaa
     7321 gaagtatggc aatggttgga agataataat acgcacttgg aacgatttcg ttggttacga
     7381 gtaaaaacgg aaggattaaa atgggaaaaa attatggaac aacatcatca actatttaaa
     7441 gccttgacca tgcaagaccc tgaagaagct aacttcttaa catccttgca tcttcattta
     7501 atgcttgatg aacaaaatgt tgtattgact actcacccaa aatattttaa aaatatataa
     7561 ctgctaaatt tcttttttt
//
LOCUS       DADOZM010000123         7555 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.127, whole genome shotgun sequence.
ACCESSION   DADOZM010000123 DADOZM010000000
VERSION     DADOZM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.127"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(102..626)
                     /locus_tag="J8I47_002303"
     CDS             complement(102..626)
                     /locus_tag="J8I47_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323726.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="J8I47_002304"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="J8I47_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAZ6323727.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="J8I47_002305"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="J8I47_002305"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAZ6323728.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="J8I47_002306"
     CDS             complement(2348..3028)
                     /locus_tag="J8I47_002306"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAZ6323729.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="J8I47_002307"
     CDS             3154..4023
                     /locus_tag="J8I47_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6323730.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="J8I47_002308"
     CDS             4049..4564
                     /locus_tag="J8I47_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323731.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="J8I47_002309"
     CDS             complement(4611..5174)
                     /locus_tag="J8I47_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323732.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5408)
                     /locus_tag="J8I47_002310"
     CDS             complement(5229..5408)
                     /locus_tag="J8I47_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323733.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5485..5994)
                     /locus_tag="J8I47_002311"
     CDS             complement(5485..5994)
                     /locus_tag="J8I47_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323734.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6090..6470)
                     /locus_tag="J8I47_002312"
     CDS             complement(6090..6470)
                     /locus_tag="J8I47_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ6323735.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6800..7549
                     /locus_tag="J8I47_002313"
     CDS             6800..7549
                     /locus_tag="J8I47_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAZ6323736.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2144 a         1567 c         1339 g         2505 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt
     5401 ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta
     5461 aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc
     5521 atctattaat cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt
     5581 cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt
     5641 gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg
     5701 tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg
     5761 gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc
     5821 agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat
     5881 ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat
     5941 accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca
     6001 ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa
     6061 acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct
     6121 ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat
     6181 tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca
     6241 agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca
     6301 aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg
     6361 cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc
     6421 tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc
     6481 ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga
     6541 ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag
     6601 gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt
     6661 caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt
     6721 ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg
     6781 tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg
     6841 ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct
     6901 tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt
     6961 attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc
     7021 tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca
     7081 gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa
     7141 tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac
     7201 tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc
     7261 gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg
     7321 ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta
     7381 tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt
     7441 gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg
     7501 tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaac
//
LOCUS       DADOZM010000124         7531 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.128, whole genome shotgun sequence.
ACCESSION   DADOZM010000124 DADOZM010000000
VERSION     DADOZM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.128"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            132..1142
                     /locus_tag="J8I47_002314"
     CDS             132..1142
                     /locus_tag="J8I47_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ6323737.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1211..2155)
                     /locus_tag="J8I47_002315"
     CDS             complement(1211..2155)
                     /locus_tag="J8I47_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ6323738.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2139..4859)
                     /locus_tag="J8I47_002316"
     CDS             complement(2139..4859)
                     /locus_tag="J8I47_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6323739.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4856..6076)
                     /locus_tag="J8I47_002317"
     CDS             complement(4856..6076)
                     /locus_tag="J8I47_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAZ6323740.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6357..7202)
                     /locus_tag="J8I47_002318"
     CDS             complement(6357..7202)
                     /locus_tag="J8I47_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ6323741.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2261 a         1410 c         1189 g         2671 t
ORIGIN      
        1 gcgtatttcc taggaatgtg atgaaatttt gaattttgtt tgaaactacc acgacaacgt
       61 ttttaaagga aatgggatat ggtatagtta atatataaaa ataataacta attacaggag
      121 tgcaaatttt aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg ttggctgttt
      181 taactctagc agcttgttca ggcgatacta ataaagatat cgcaacgatg aaaggcggaa
      241 cgatcactgt atctgatttc tatgatgaag caaaacttga atcatcgaac caatccttag
      301 tacaacggat gatcatttat aaagtattca ataacaaata tggcgataaa gtaaccgata
      361 agcaagtgga tgcagaatac gacaaacaag cgaaatcatt aggtgatact tttgaatcac
      421 aattagaagc tgctggatat acgaaagata cttataaaga atatattcgt aacaatttag
      481 cttttgaagc tggcttgaaa gctcacgtag atattacaga tgacgatttg aaaactgctt
      541 ggaaatcttt ccatccagaa gtagaagcac aaatcatcaa attatcaagt gaagatgaag
      601 caaaagatgt gaaaaaatca gcggatgatg gagatgattt ctctaaatta gccaaagaca
      661 aatcaactga tacagagaca aaagaagatg gcggtaaagt taaattcgac tcaacgacaa
      721 caactattcc tgcagaagta aaagaagcag ctttcaaatt gaaagacggt gagatctccg
      781 atgttattac tacaacaaat cctacttctt atgcgacaga atactacgtt gtcaaaatgg
      841 tgaaaaacca aaataaaggc aatgacatgg ataaatacaa agatcaacta aaagatatcg
      901 ctactgaaac taaactaagt gacaatgcat tcacaactaa agtaatcggt gaagaattaa
      961 aagatgcaaa tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa
     1021 caacttcatc aagtacgaaa gactcaagtg aaactacagc ttcaacaaag agttctgaca
     1081 caaaatcaac tgattcaaca aaagaatctt caactgaaga aacaactgat tcatcaaaat
     1141 aaaagaaaac aaaaaaagcg aagacttatg tctcgctttt tttgttttga atgaaaaaaa
     1201 taaatttttg ctataaatct tttggtacat agaaacttcg gttgtccata ccgaaaatcc
     1261 gatcggtgta ttttcctggt tctgtttgac gaatcgaagt caacatcatc tgcatcgaag
     1321 catcgatatt gtcgatttga tgtaatattt ctgcttccat tattcttgga cgtacaggtg
     1381 agccatattc taataagcca tggtgtgcta aaaccatatg acgtaataca agaacatctt
     1441 cgtcagcttc gctgattttc aacgcaagac aggctttagt tatttcttcg tcaatcaata
     1501 ccagatgacc gatcaaattt cctgcaagcg tatattctgt cgacatagga ccggaaagtt
     1561 caatcacctt tcctaaatca tgcaaaatga taccagcata caacaatgct cgattcagct
     1621 caggatattc atcggcgatt gatttcccta agtgaagcat tgtcaaagta tgataagcta
     1681 atccactggc aaatgcatga tggtttcttt ttgctgccgg aaactcaaaa aattgttttt
     1741 gatatttatt aagtagatag cgaacaatac gattccaatg cgcattggtg atctcaaaca
     1801 acaattgatt gatttcttct tccatttctt ctttcttcaa aggggctctt tccatgtata
     1861 aactaggctc tcctggctca tctacccttg ttaagcgcat atgaagaatc tttacttgag
     1921 ggtttccttg ataaacctct cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt
     1981 tattgatttc ctcctccgaa gcatcccagt atttaccatc gatagtacct gaagtatctt
     2041 ggaaagtaaa agcaataaat ttttttccgt ttttagcgat tcttacatct gcacttttga
     2101 tcaggacaaa ttgttcaaac tgctcatcga ttactaaatc acgaattttt ttcattattt
     2161 gctccttcta attgttttac gatttggttc atctcatggt aatagcttac catttcttta
     2221 tcagaagaaa aacaaattat ttgatacttt tctccaaatt tagctaaaag ttcagctaat
     2281 tgatactttc gctgatgatc ataatgaagc cagccatcat caatgatgat cgggcatatc
     2341 gaacggttag cttctaatga gaggtaagca aatctcatcg ccataatcag ctgatctttc
     2401 gtacctgtag ataactcata gatgggaaat tctgtatatc cgtctgttaa agtcagtgta
     2461 tcatttttca gtaatatctt ctgataattt ccttttgtta aaatacctaa ataatcacct
     2521 gctgctctta acaactgggg taattgtctt tcactcatct ctgttgaaag atcacctaag
     2581 aatgtcccgg cgatttcaag tgctccccat tcctccagta attcctgtaa ttttgctttt
     2641 tgccggctag cttgttgata aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact
     2701 tgaagttgga gtcgttgccg attttcactt gcttcttttt cttcttgctg gagttgggat
     2761 tgcttttctt tcagttggcg cagtcttaca gataatttct cttttgtcag ctcttcagga
     2821 aatagtggag taagcatttc cgataattca ttgtatcttt tcatctgctg ttcggcttgt
     2881 ttggcttttt gtaaaaagac aggtacttcc gaaggataag ataagcctgc ttgagtcaat
     2941 tgttcgctgt attcttgtat ccatactttt tgtttctttt tcaattgatt gatttcttgc
     3001 ttcaataaag tattttgctg atagcttctg gcaaatttca tctgttccat ctcttgtata
     3061 aaacgggaaa gcaataccat tttttctgaa attgtcttat cttgtatcgg taaccattct
     3121 ttaacaaact caaatttttg gtcgatttgt cgtagttttt cttgtatttc ctgttctctt
     3181 ttatgtttcc gctcaaatac gccatataat ttctgataat ctatgacttc ctgtccatat
     3241 tctttcatga gtttgagcgt atccatcttt cctaagtgcc gatgatccgc aaaacgatat
     3301 acttcttgct gaagaatctg tttttcttct ccagttctct tttgtttttc ttctgcttca
     3361 gctaattgag ctaaggctag atcaagttgt cctagttttt cttgccacat tccttttatc
     3421 tcagagaaat catttttagt tttaattttt ggttgccttt tgtagaagaa atagccaatc
     3481 atgcctgcac caattagtaa aaggatcact ttcaagggca ttggtacaaa tatacctgca
     3541 atgattattg ctccgccagc catcagcata tacggtttag atgaagagac tggtgctatc
     3601 tctgactgat tttgtaataa tgctggatag gtctcttcca agtcattcac ttctgcttca
     3661 atcatctctt gctgttcttt agctatttgg agacgcacag aaatctggct ttctagatca
     3721 gcaatccttc tttcttcagc cagaagatgc ttccattctt cttcatataa aaattcttcc
     3781 ggtaacggat ttttctgcca atgccacctt tgttccaaaa tcttcaattc tgaatttgtt
     3841 tctctagtct caatttgtaa tctttgccat tcttcgttta attgttccgc tgtaaattga
     3901 agttgctgga gatcttttag ttcctgctcc tgctctaaat aaaaataata tctggctgac
     3961 tgctgatcca tcccgctatg cttttccagt gttgcttcta attcttccaa acgctcattt
     4021 gtttgctgat accgtttgga gaaagactcc agatctaaaa gaagttgctc atcaggaaga
     4081 cctgtagtgc tgtttttttt gagagattgc cattcttcat ataaagaaaa attaagttct
     4141 tgctgtctgg ctaatgaaaa ttgtttgttc gcctctttta attgtgccaa taactgctgc
     4201 tgttgcgact cgtattcctg ttcttgttgg atcaacttgc caaaataagc ttcctgttct
     4261 tgtttttgat gaattttttc ttgcagctct ttccattcat ttaatttttg attgattagt
     4321 aatttttggc cttttttctt gaataattgt tgtgctttct gataatattc ttgccgcttt
     4381 tggaatactt gagaactgcc cgtgatgcct aaagaaatca gcgcatcatg tagctctttt
     4441 tctctcaaat gatctagttc catcaactgc tcttgctgga aagtaaatac ctgctggaat
     4501 aactctttcg tcaaaggtgc gatcagtttt tctagtaact catcacttcc cacttgatcc
     4561 cccagccaca ccttcgattt tccgcgattt tgctgcttgt agcgctcgac agcaaattca
     4621 ccatatacag gatgtttaag ccatattttg ccgccatagg ctgagccgtc ttttggtgta
     4681 taatcctttt ttttacggcc ttttgaaggg aaaccaaata atattgcttg aataaattga
     4741 taaatcgttg attttcctgc ttcgtttccc ccatagatca gctgattgcc tgaataaaag
     4801 tcaaaacttt tttgacgcca ttttccaaaa gcagcaatct cgatccttaa aatcttcatt
     4861 cctgatcctc ctcaaataaa aaatcttgat taagcagctg cttggctttt tcaagtgttt
     4921 cttcttgata ttccggcagt tcgctcaata ttctagccgc ttcttgatga ctatatactt
     4981 catttaaaat ttgttggaaa tcttgaggtg tctgatagta ttgaaataac tgttccatca
     5041 atttagcaga agcagcaagt ggggtcttcg tataaaaagt attctctatg agagaaatac
     5101 gccaaataaa gaaatcattt atcctatctg agaattcctc taacaaatag tctaatagct
     5161 ctcctgattg tatccgttcc aaaacatctg tattgagatg gtcataatct tttaatttta
     5221 tctcaattaa actaaatcca tcctctactt ttgaaagctg ctgacgaatg tgaacaatga
     5281 cctcttttgt cgttcttgct ttcctcaatg atatttcctt tgtcttccaa tagacctctg
     5341 ctactttaat cggatgtatt tggcaacttc ctttacttaa ttcaactagt agaatagatg
     5401 tagaagcttg ttccttttta gtatgacctt gaggcgcacc tggataaaca attgtttgct
     5461 tttcatttaa gaccgttggc acatggatat gccctaaagc ccaataatcg tatccctttt
     5521 cttgcatctt tgagggttgg aaaggagcat agtttccttt ttgagagacg ccaggttccc
     5581 cgtggtacaa cccaatatgg taatctgtga gttctctaga aggaaattcc atcactttgt
     5641 ctttttgaat ccacggttga cgataactaa atccactgag agagacagtt tctccacttt
     5701 ttatcgttat tttttttgtc tctaccgttt cggatgtaaa taaatggaca tttttaggaa
     5761 aggagaacca ataccgctct ttttgataga aatcatgatt tccgaatatt ataaatacag
     5821 gtatatggtt cttttctaac cgtttcatct gttctgaaaa atgtttctgt atttttaatg
     5881 aaggacgatt ttgatgaaaa ttatctcctg caagtaaaac aaaatcaacg ttatttatta
     5941 ttgcctgttc aatgatattt gataaaacgg tcagattcgt cttcaatagt ttttcctgta
     6001 cagttttatc aagagttgtc agtccttcaa atgaccgatc catatgtaaa tctgccgcat
     6061 ggataaattt caccattaaa aatttcctcc tttttttctt ttatcatacc attttcaatg
     6121 ttttttttca tcaataaccg aaaatttgtt cgtattatgc aaagaaatta cgataaatgg
     6181 ccgacttcaa aaaataagtt tattcgaaaa aaagattgtg acatgatttt tgtcacaatc
     6241 ctaaatatct atgttatatt cgcttcgttt gacactgaag aagtactatt tgtactggaa
     6301 tttatgctcc atttttccgt atttagaatt ctatgttcta tactacctta attagtttat
     6361 atggtaagaa ttctttcaaa tgcttgagat actccgtctt tatcatttga aacagtgaca
     6421 taatcagctt ttttcatgat ttcttgatga ctatttccca ttgcaaaact aatatctgca
     6481 ctttcaaaca tagataagtc atttaaacta tctccgatcg cgattctcaa aggtttcttt
     6541 aaattgtaga ttgattccaa acgatcaaac accacacctt tagatacatt ttgaggtacg
     6601 atttcaatat ttacaggtgc cgattcagta acagacacat ttagcatctt tttcaaatgg
     6661 ttaaatatat tagctttcaa ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg
     6721 atagcatcct tttctataat ttcctcaata ttattataag ggattgaaca aatagatttt
     6781 tcatactcaa gctcatagtt tagctctttg atagaaaatt cactccctac ctcgtgaaaa
     6841 atttttttca ttttattttt ataattctga gattcataac ttccgagatt tgtatatatt
     6901 ttggttggaa atttcgatag gacagaatca atttttttta catcattttt atcgaaggaa
     6961 gactcaaata tctttttttc tccatcaaat ccaacagaac catttaaccc tactgctggt
     7021 atccttaaac cagatttttt gagaatatta ttaatgtctt ctacttctct tccagaacaa
     7081 atgaatggaa aacagtcttc cggtaaatgt ttcattgttc ttgcattttt ttgactaatg
     7141 ctctggtcag aattcaaaag agtaccgtct aaatctatac caattagttt catttcatcc
     7201 atgtctccta tacgttgatt ataaattcac tgcttattgt aagtctaaat ttctcacaaa
     7261 acatatacta attataccta atttaagtaa gtacaaacat cattttaaga ataggaaagg
     7321 acatttatat actattctct gtatttttga tttataaata aatatgaatt gaatccaaat
     7381 cagctatatt actattttat aataaccaac tataaaaaac gaagttaggt gaaaaactaa
     7441 cttcgatatt cataaaagac tctattagtt caacttagca aaagatttaa acaaaaaaag
     7501 atgaactatt tctagttcat ctttttgcct t
//
LOCUS       DADOZM010000125         7513 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.129, whole genome shotgun sequence.
ACCESSION   DADOZM010000125 DADOZM010000000
VERSION     DADOZM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.129"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(59..787)
                     /locus_tag="J8I47_002319"
     CDS             complement(59..787)
                     /locus_tag="J8I47_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6323742.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="J8I47_002320"
     CDS             complement(800..2080)
                     /locus_tag="J8I47_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ6323743.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="J8I47_002321"
     CDS             complement(2221..2823)
                     /locus_tag="J8I47_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAZ6323744.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="J8I47_002322"
     CDS             3068..3274
                     /locus_tag="J8I47_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323745.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="J8I47_002323"
     CDS             3586..4305
                     /locus_tag="J8I47_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ6323746.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="J8I47_002324"
     CDS             complement(4386..5069)
                     /locus_tag="J8I47_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAZ6323747.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="J8I47_002325"
     CDS             5287..7473
                     /locus_tag="J8I47_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ6323748.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2693 a         1271 c         1412 g         2137 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaa
//
LOCUS       DADOZM010000126         7485 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.130, whole genome shotgun sequence.
ACCESSION   DADOZM010000126 DADOZM010000000
VERSION     DADOZM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.130"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(35..214)
                     /locus_tag="J8I47_002326"
     CDS             complement(35..214)
                     /locus_tag="J8I47_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323749.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(595..1302)
                     /locus_tag="J8I47_002327"
     CDS             complement(595..1302)
                     /locus_tag="J8I47_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323750.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1295..1732)
                     /locus_tag="J8I47_002328"
     CDS             complement(1295..1732)
                     /locus_tag="J8I47_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323751.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1747..1998)
                     /locus_tag="J8I47_002329"
     CDS             complement(1747..1998)
                     /locus_tag="J8I47_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323752.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1998..2204)
                     /locus_tag="J8I47_002330"
     CDS             complement(1998..2204)
                     /locus_tag="J8I47_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAZ6323753.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2641..2901)
                     /locus_tag="J8I47_002331"
     CDS             complement(2641..2901)
                     /locus_tag="J8I47_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323754.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2976..3179
                     /locus_tag="J8I47_002332"
     CDS             2976..3179
                     /locus_tag="J8I47_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAZ6323755.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3320..3805)
                     /gene="ssb"
                     /locus_tag="J8I47_002333"
     CDS             complement(3320..3805)
                     /gene="ssb"
                     /locus_tag="J8I47_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ6323756.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3808..4707)
                     /locus_tag="J8I47_002334"
     CDS             complement(3808..4707)
                     /locus_tag="J8I47_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAZ6323757.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4720..5313)
                     /locus_tag="J8I47_002335"
     CDS             complement(4720..5313)
                     /locus_tag="J8I47_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAZ6323758.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5554..>7485)
                     /locus_tag="J8I47_002336"
     CDS             complement(5554..>7485)
                     /locus_tag="J8I47_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ6323759.1"
                     /translation="VVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADHGKIFKRL
                     WINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIP
                     GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQ
                     EAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTL
                     EAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYV
                     NNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILT
                     QVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKET
                     KPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKV
                     EKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFV
                     SHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTS
                     EACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGF
                     EFSEKKKTNYKKRTRRTTK"
BASE COUNT         1891 a         1535 c         1292 g         2767 t
ORIGIN      
        1 ctatcacttt tagtttttag gataaaaagt gacgtcactt tttgagtgct ggtacctcaa
       61 atcctgcttt ttcttgggca atcaataaat ctaacgcaat acacataatc ctggattcac
      121 ttttgccgta cttctgcttt agttgctgca attctttttg tctcttttca tccaacgtca
      181 cgggtaaccg tttatttcct attgcaattg gcattagtac tctctccttt catcgaaata
      241 accctattaa aaatatggct gcaatcaact tgaatattag actcacaagg gttaacacaa
      301 agcgccaaaa gatcaaacaa aataccagta ttcctaagaa tactacgaag tttaaatgta
      361 gagctgcacc atgacgcata agtatctcgc caacaaacag tacgaacaat agaggaatga
      421 ttttattcat atggatctcc ttctttctct taggttatcg ggctttgccc gcttatttcg
      481 caccacctaa gcggcggggg cggttggatc gattggtgcg aaataagaac cattccctta
      541 atttcaaggt tttttgttct tatttcacac atcctcactt ttcggtataa atggttattg
      601 accttttttc ctttcaaatc gttgatatgg cattcgttta cggtcaagtt cattttggat
      661 ttcttctgta tcgtcgtaag tgtaggtgtc taaaaaatca gttaaagtcg tgtaccagtc
      721 gtcgcctggg ttatctactt cttctacata ttccctcaaa aatgcttcgg gtacagtgag
      781 ttcataatcc attcccatta acacgataga ttccgtccca cacgtcaaaa atctagtaaa
      841 tacaaggtaa taatcgtcta ccgtcgcatc tttgctgttt agctcttcca tttcttcttc
      901 cattaattcc atgctctcaa aaattgaata atattctgat tctgagtaat agtgtttcct
      961 gcaaatcgtg tagctgacaa agttttcgac cagctcactc ctttctaggt aaacattcat
     1021 gatatatttt tctgtttcta gtcgtagaag ctccgctcct ttttttaatg tgtcaggacg
     1081 acgtttatac acgtctaaac gattatggct gactgtaacc ttcgtttgac tcgtttcact
     1141 ttccatatac aactgttctt ccactctttg aataaagtac ttcatttctt ccatacaact
     1201 aaagtctttt tgaatccgtc tgattacttc attcaagtgt tctttgtttg ttaaaaattg
     1261 cgaaacatct tctgttgtta gctgataaac caccttattc attatctttc tcctccgatt
     1321 caatcgtata aaatgcacca tttttttcga tattttcaaa agtatctagc cagtcgctag
     1381 ccattgcttt agctttctta atctgtgcga attttgcata agtcagttct aaggcttgat
     1441 attcggaagg tttgatagtg cttggtttga cacctacttc caagcatagt aaggcttgga
     1501 aaaaccattt tccattttgt tttttcagtt cttccagtgt tcgatagtac gtcgcaatga
     1561 gattcatttc gacacctgat tcaatcattt cttccactgc gactgacaat ttttccttgt
     1621 aatgtttttt gggtacacgt gtttcatggt ataggaacgt catatcctcc attttttgtg
     1681 ccattttatc caaaggtaat ttgacaaatt cgtcatagta attttgagcc atattttttc
     1741 tcctttttag ttctctaatt tggttaacca atattcgatt tcataagttt ccatgttttc
     1801 ttgataatgc agttcatatc ctgcaatctt cactaggtct tcaaatagca tagtcggtgc
     1861 atcctctact aaaggggttt ctatcgtcca gtaccgcgga ataagtaaag cttctgctac
     1921 tttttcagaa atgacttctt tgtccaccaa aatttgaacg acttctttta cagtcaataa
     1981 tttgtgttgt gttttcatca ccgtttcttt actttctcat caatcaaaac ataatcgcca
     2041 taagacaagg tatatttttt cccgcatgtt ggacattgat acaaacgata gcttttgata
     2101 catttcgctc tttctccaca atcgcattcg acagtatgtg gtgcatgata gtttgggaat
     2161 aattcgctga agggaacacc ccaataatca aatttctttc gcatgattga ttctgcctcc
     2221 tcttaggaaa ggttttattt cctttcgtta gttctttctt tattgatttg ttggccagaa
     2281 tgaatcaacg aaaagcgaca aggacaaggg cgcgcagcgt gccatttacc cttgtccttg
     2341 tcgctttttg gtgataaaag gcaacttgtt gccggtctgg caaatcaata aagagatagc
     2401 gaacaggaac gaaccttttg gtgagaggaa gttttttcta ttttgtcttg ctttgcaaga
     2461 caccatatct tagtgaacgt gcaacgttca cttcctttct tctcgaacgg cggtggcggt
     2521 tggagtgaga gaaaggtttt tcaattgaga aggcacttgt gtcttcctaa aaaggtcttt
     2581 tcctgtttct atgtaaaaca aaaagagctg caatcctcat ttaggactgc agctcttctt
     2641 ttatatgccc atcgatcgcg taaaatgatc ggggtttcta atttgcatcg attcaatgac
     2701 taattgattc cgtttattga aatgacctgt gacggcaata ttgtattttc catcatccat
     2761 aattaacaat ttatcgataa tttcaatatc tgcaactaca caattaatcg attcatgaat
     2821 tgtttgtaaa gtaaagcgaa ccaataatgg ttgtgttttt attactcgga ttttatcaag
     2881 caaaccaaca tatttgttca tcacgatcac ctcgctaaaa ttatagcaaa aacagctaaa
     2941 attgagaatt tttattcgat tttagatatg ctataatgat caagacagta aagtttgtga
     3001 cggtggctat ccgattgaaa gtgaggtggt gcgtatgtgc gtacttccaa aatcttacag
     3061 aaaggagaag ccttttgcct gttgaaacgg cgctaggatt aatgattagt ttcggttctt
     3121 tgatcgcaac aatcattttt ggaatcttag cagttgtaaa caacaatcaa aaaaaataac
     3181 cgtcagcttt agccggtcga cgattatttt tcaaaaataa tattaagctg gtcaccgtct
     3241 taaacggtgc tgttagggga gttgttagcg caactcctct tttcgtatcc attataacaa
     3301 ctttattgat cttttacaat cagaatggta aatcatcatc actaatatcg attgattgtc
     3361 cgccaaatgg attttgtgaa gcgttcaatc cattgttgtt aggcgtagct acatcccgtt
     3421 gttcaaaggt ggtagggctt ggactgctgg acgattgatc agcgtgcgca cggctttctg
     3481 tcgctgcttt actttctaat agttggaaat tatcggccac aacttctgtc acatacacac
     3541 gttgtccttg ttggttatcg taggttcggg tttgaatccg tccaacaacg ccgatcaaac
     3601 tgcccttttt agctaatgtc gccatcgttt ctgctggttt gcgccaaatc acacagttaa
     3661 taaaatcagc ttctcgattg ccgttttgat tcgtgaaatt tcggttgaca gctagggtaa
     3721 aagtcgctac tgcagtacca ctcgcggtat aacgtaaatc tggatctttg gttaagcgtc
     3781 ctactaaagt tacattattg atcatcttta tctctccatt tcttgattca tttgttgtga
     3841 tcttttaaac tgccgttttc tttcttgatt ttcaattttg atcacacttt tcattaggtg
     3901 atcaatttcg ttgactgtat ctgtaaatga aatggaattc gcaaacgtgt taactgagcg
     3961 atatttttcc cactgctttt ccaaatagtc atttcctctg atcttatcca tttctctttc
     4021 ataataaaag gacaacggtt ttgtatcccc acgttttgta aatgtatttc gaacactttt
     4081 gtataactct ttcgtatcga tattttctag tttcggaatg gtaaataagt cgtagtaatc
     4141 ttttgctctc gaattatctg tgccaaagct gaacgtagca cttagctttt ctgcaataat
     4201 ttgttctgtt ggataagctg gaatctccac ttttttatct tcaaacatta attggtgata
     4261 gtgtgtatct acttcaggat aaatagaatc tcctgttgtc acgtccattt taaacggtac
     4321 acgtactttc tctaaattag ccaccaattt aatttgaaat ccaggataaa aatcggcttc
     4381 tcttgtttcc ttcattcctt gaatagagaa aacaatgcct tcttttgtgg gttttctagt
     4441 taaatcatta aaaatgctct tcaatttttc ttcagttaat ttgctatttc taaatgtcgt
     4501 atcgatatca attgttgtcc ttctatcgat tccatatttt gatcctaata aaaagccgcc
     4561 tttcaaaatg aagtcatttt tgtactttga ttcagaaatt tgttcaatta gacgatccat
     4621 cataaaatgc cgttgcacta actgtgcatt taatcctgta ctctttgcta aatttcttgt
     4681 ttttgctgca aattgtgtcg gtgtcatctt tttcctccat caatataagc tttgcatgtg
     4741 gtatttcatt tctttttcta catgtagttt tgtcatgtaa tctcttaatt tacttggatc
     4801 tctcaatggg tcttccatgt actcttttaa tgcttctaat ttcatttcat attcaaattc
     4861 atatctcgga ttccaaatat cacaaagtgt acgctcttta tcatacatag taataggatt
     4921 tccttgaaag gaagttccct ctacttttcc tagttcataa acttctttct tcacgaaatg
     4981 aaattgtaac tgctgctctt ttgtaattga acgatcaacg ttatagccat ggggaaaagt
     5041 taagtgaatg aatttcggca tattcgtaga aagctggtaa atatctaaag tcgtttcatg
     5101 agagaagatt cccctggaaa atcgttgctg aagccaaaat aaatcatcta ttccatgacc
     5161 atcctctaca taataaccac gatcaacttt gatcaatttt ccttgattca ccaatctttg
     5221 tacagtggtt gctgcgattc caaatttcct ggcttccttc atagataaat ttccatcgcg
     5281 aatagccaac aattctttca gtttatcttg catttacttc ccctcccttc gttacaaata
     5341 cactacatca aatatatcat agtagtttat ttgtaacaag attaacgtct atcgatctaa
     5401 ttcgatttct ttttgaatgc gctgctggtt ctttaattgt tgcagtcttc ttttatttgc
     5461 acgtaatttc tcttgttcta aacgaatttt tgctttttga ttttcgattt cttgttgttt
     5521 gtgtgttgta tttcgcatag gataacccct tcattatttt gtagtgcggc gtgttctttt
     5581 cttatagttc gtttttttct tttcagaaaa ttcaaagcct gttgtaaagt cttcttttaa
     5641 aacgatcgtt gcttcaaacg aacctttctg accttttagt cctctgactg attggcttgt
     5701 tcggccgttt tgaatgatct ctttcagttg tgtggctgtg agtttctttt tggcaatcgt
     5761 tgtccataat ttaaaatcac aggcttcaga cgtacatgcc gccacatttt gataaagcaa
     5821 gacttcttgt tcccgacatt ttggacaaac acctaaatga cgcgttgctt cctgtttttc
     5881 actggctgct ttcacctctt gtaacgttga tccaaaatct gttgactgga tttgtcccgg
     5941 tacgacttcc aacaagtgag agacaaattt ttggatatta gctaagaaag tagcttgtgt
     6001 tcctttctgc tcaccaatct ttaataagta actttcccat ttggcggtca tttcggcact
     6061 tgttagcagc ggctcttttt ccacggcttg gcaaagtaaa atccctttat ttgtcaccac
     6121 aagcttattt ttttctacct tgatatattc cttttgtttc aaagtttcaa taatatttgc
     6181 tcgtgttgct tctgtaccga ttccttctac ttctttcaat atggattgtg cttcttcgct
     6241 atccacggtt tttccagctg ttttcatcgc tgtgatcaac gtaccttcgg tataaggttg
     6301 tggtggcttc gtttcttttt caaggacttg tagatctacc gtcaccgctt ctccttttgt
     6361 cactttagga agtagcggtt cttcttcctt ttcttttctg tcgctctctt tgccaaacag
     6421 ctttttccaa cctagatcta ctggtacttt tccgactgtc tttagctgaa gctgctgaac
     6481 ttgcgtcaag atcgttgttt cttcataggt atacttttcc gcaaacatgg ccaccgttgt
     6541 ttttaacact tgttcataaa tagcctgctg cagtggactt aattgttcaa aagccgctgc
     6601 cgtcatgacc gtctttgtag gaatgatcgc gtaatgttcc tggactttct tattattcac
     6661 atatcgacta ttcggtgtat gaataggagt aggaatggct tcggccttta aaaagtgttt
     6721 gtattcgtct aagtgatcca acagatacgc atactcccct tctgtaatat aaggggtatc
     6781 tgttcgtggg tagcttagat atttcccttc atacaaccct tgcatggctt ccaaagtatc
     6841 tttggcacta gctttcatga gctgattcat tttagattga aggctggata gagaaaaaag
     6901 acgtggactt gccgcacttt ttttctcggt ttcaacttgg tggatcgtac ctggctgttt
     6961 tcccaattga accccctttt cttttaaata atcttcaaat gcttcttgcg tcgcttgggt
     7021 ttcattcgga tcaagcttcg catcaaatgc gccattttga gcgattactt gggcttttcc
     7081 ttcaaaatat ggttcttttt ggaagtttct aatcttttcc tgtaattgat aaatcatgta
     7141 aagcgttggg gtttggactc tgcctaagga aaaacttcct ggtatgcctt tttgctgcaa
     7201 gagtaaacta tacagcgggc taccgttcat gccgatcaac caatcggcga tctggcgtgc
     7261 ttgtgcttct tcaaattttg ggtaggtctc ttctgcatct cgtaactgct ggaatccctg
     7321 ataaatggct tctttttcta aggagttgat ccaaagtctt ttaaatatct ttccatgatc
     7381 ggcgcctgct tgttgaatga tcgtccaagc tatcagttcc ccttcacgat cgctatctgt
     7441 cgcaataata atggtgtcgg ccgattgaag ttgctgtttc accac
//
LOCUS       DADOZM010000127         7475 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.131, whole genome shotgun sequence.
ACCESSION   DADOZM010000127 DADOZM010000000
VERSION     DADOZM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.131"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(746..1786)
                     /locus_tag="J8I47_002337"
     CDS             complement(746..1786)
                     /locus_tag="J8I47_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAZ6323760.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2135..2461)
                     /locus_tag="J8I47_002338"
     CDS             complement(2135..2461)
                     /locus_tag="J8I47_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323761.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2448..3236)
                     /locus_tag="J8I47_002339"
     CDS             complement(2448..3236)
                     /locus_tag="J8I47_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ6323762.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3604..3894)
                     /locus_tag="J8I47_002340"
     CDS             complement(3604..3894)
                     /locus_tag="J8I47_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAZ6323763.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4030..4209)
                     /locus_tag="J8I47_002341"
     CDS             complement(4030..4209)
                     /locus_tag="J8I47_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323764.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4206..4556)
                     /locus_tag="J8I47_002342"
     CDS             complement(4206..4556)
                     /locus_tag="J8I47_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323765.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4549..5874)
                     /locus_tag="J8I47_002343"
     CDS             complement(4549..5874)
                     /locus_tag="J8I47_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAZ6323766.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(6245..6829)
                     /locus_tag="J8I47_002344"
     CDS             complement(6245..6829)
                     /locus_tag="J8I47_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6323767.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(7040..7243)
                     /locus_tag="J8I47_002345"
     CDS             complement(7040..7243)
                     /locus_tag="J8I47_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323768.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7253..>7475)
                     /locus_tag="J8I47_002346"
     CDS             complement(7253..>7475)
                     /locus_tag="J8I47_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323769.1"
                     /translation="IVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGY
                     WYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT         2279 a         1359 c         1085 g         2752 t
ORIGIN      
        1 caaaaaagag ggaacgcatc caaacatgcg ttccctctaa tttcagcgtt cgtcatacgg
       61 caaagagcca cacggacatt tttataacat tatgcttagg catagattca tcgtaatatc
      121 acgtattttt aaccttaaca cagccaaaaa aagtggtatc acttttgaat atctgttata
      181 ctaaatccgt tacgggtaat accgtaacac tgaaaggtaa acgcattaag ggagtgatct
      241 taatgccacc tgtggacgtc gacgaaggtt accagccttc cgagtctcac agggcctttc
      301 ttttttttgt attttcaatt gtccaaattt atcgtccaaa agtgcagaac gaaaatcaat
      361 tcatcacaag catagcagac aattaaattt aaaacaacta aaaatataca aaaaagtgta
      421 attgaatatt agcagcactg cctgaaaaga agtaatatca ggcttttcag cttggggaaa
      481 taagtgaatt acctctcttc atgaattatt ataactcaaa aaagcacata tgcaaacact
      541 agttccctat ttttttgagt gaaaaataac taaaatatct aacgattatg aatttaaaac
      601 ctacgattgt gattgttttc ataaactttt aatctaaaaa ctccggaata ctaactattt
      661 atttgtaata tttgtaataa aattaaaatc ataaaaaagt tctaatttaa aattgatcat
      721 ttttatagta attgcttttt atcaactact ctaaccaatt ttccgtacta actgttggta
      781 attcgttaaa tgtttctctt tgttcgtttg caagctttgc cgcaatagct ttttcgacaa
      841 accaattttt tacataaacg aaaattagat tctgcaaatt gtttactttt tcagtttttt
      901 gtttttgata agctttcagt aaagtcatat ataatccttt gtcagcatca attccataag
      961 attccagttc ttcataaacg acaatagttt gctctaattc ttcagctttt ttctttgcat
     1021 tatgaatagt ttggacggtt tgttgagctt cactgtaact tgaagaaaat gtttttatca
     1081 agtttaatac attttctggt acaaatgtat ttatactgtc ctcttgcatg atgtcgacga
     1141 agttatctag caaaacttga ttttgtaact ggtcagtttc agtgtctatt atgtgtctat
     1201 tagtgtctat agaattagaa tagaatagat tgtctgcgat tttcgcaatt acggttgagt
     1261 ccttgatatg actggatttt gccgtatttg cgattttcgc aattacggta gaatcattga
     1321 tttttcgcgg ttttgccgta tttgcgattt tcgcaattac ggtggaatca gtattgtctt
     1381 cctcttccat gattttaaag acgtcttgat tcgtaacttc tggcttgcct aagtacaata
     1441 aattaggcat agattcaatc ttaccatcac gtttatttac tcttcctctc ttctgtttca
     1501 gaagacctgc tttttctaat tcttttttga tcttagatac tttaccttcg cggcattgta
     1561 acaatttcat taattcttca actgtgaata tgaagaatat attatcatca gaatctaccc
     1621 agttattttt gactgaataa tcaaatcgat ctttaagtag catataagca atttttgcat
     1681 catttgaaag atccatgtat ttttcgttag taaaaaatac cttaggcatt tgataatatt
     1741 tttcacgata aatttctttt gatttgtaat aattaaaatc attcatagcc aatcctcttt
     1801 ctgtaggata gcgttttgtt caaaactatg ttacaatgaa ttaaacaaat atatctatcc
     1861 actatatatt tttctactgc tgataagtcg ccaaactttg ttaaagcagt agtctttttt
     1921 tatctttttt tagtttacct taaaaaataa attaaagcaa accgctctat ttcagggttt
     1981 aagctcatgg taactatatc acgatattac aataacatta tatcacgata tcgtaatatt
     2041 acaatatgaa gataagcgac ttatcagctt tgatactact gatttttata aaaaaaatat
     2101 cacgatatta tgatatcgtg atattgaatg taaattaaat tttattttgt ttctttgcta
     2161 gtttatatcc ttcaatcaag tattcttcag gtatggattt taatagcgca tcttctataa
     2221 cgtctggttt acttttgtct gattcagcaa tagtcgtcca atatttgagt gctgacatta
     2281 gaattggatt tatacgaatg ctatctgctg ttcttacact acttccttga tcttttatga
     2341 ctttacttat atttttcttg gttaattcga tttcttctac ttcttttggt ttgtagttct
     2401 ttttattttt atttcctagt agtctactta ctgcagcttg actgcgatta tcttctgcca
     2461 tcaaaatcac caacttcttt taaatattct tctgtaaatg ctttatagtc atcttcagct
     2521 gttgagggtt tattaaaatt gattagatct gatccagcag cttgcgcttc gtcaacggtt
     2581 attgttctac gaatataagt tttgaaaatt tttgcaccaa tgactctaga aatttcggtt
     2641 gctgcagtcg tcatttcttt agaaatgtta taacgcttat tatacttagt aaataggact
     2701 cctcgtactt caagcttgtc gttcgtatat tcttttaccg tttcaatagt ttcacttaac
     2761 tttattagtc cttttaatga gaaatacgca gcttcaactg gaatgataat ttcatcagaa
     2821 acagtaaatg cgttgactgt aagcattcct aaacttggtg gacaatcaat tataatataa
     2881 tcatagttgt ccttgattgg ttgaagtact cttttaagcc tgtgttcact tcctacttga
     2941 gtaaattccc tctctgcaga agaaagggct atgtcggctg gcaaaacatc aattccttta
     3001 tagtccagaa tagcatctgt aatgtcagcc tcccctttta atacttcata aattgtaggt
     3061 tggtttgttc catctatatc gaaacctaca ttatctgtag catttccctg aggatcaatg
     3121 tccactaaaa gtacattttt accgtaaaca aatttcaaac atgctgctaa attaacgcta
     3181 gtcgtagttt tagaaactcc acctttttga ttggctactg acaatattct tgccattaaa
     3241 atatccccca ttcatcttat attataatat aattatatca taatattgtg atattgcaat
     3301 atcgtatatg cattatattg taatattgtg tgattatttt gttttaaaga tatatatcgg
     3361 ttcggatata aagattattc atatgttatt cgaaagtaaa ataatcactt tttattactt
     3421 taaaattttt tattaatcat aagaaatatg ttttgcgagc agcaactaga ataatttaaa
     3481 cattattaaa ctgtctatat tgttactaat aattcccttc ttatagtgca ttttcttaga
     3541 aaaaaacaat attacgatat catgatatta agatatcgta atattataca attgtgattg
     3601 atattatata tgttttttta tttttttatc atgtttaatc ttgaaggaat catattcttt
     3661 ttctaaagac aggagctttt cataaacatc agacatgttt aaactagagg ccaggcttaa
     3721 atcataaacg aatgaagcag gtaatgattt tattctacgt tgtcgggtta tccagcttgc
     3781 aaccgttcct tgtgggtagg catataactc acaaaattgt tctacagtga ttcctaaatt
     3841 cttgataatg taaacattga tcgggtgcga gtaaacaaat gtattgatag acatggtgcg
     3901 taagcctact ttcttttatt attgcgtttg caataatagt ttaacacacg cattattcta
     3961 gtatctactc ctatttgtaa aaacaaaaaa ggattcatag agataactct atagaatccg
     4021 taaaatacct tatatatata acgggaaaat ataaaaccta aaattatgat aacctgctgc
     4081 agcttctaca tagaaagaga agcgctcaac accaacctca tttatcaaac gttggccata
     4141 accccaaatc tcttctgata gctgatcaaa ggttatatac tttagctcat attgctggat
     4201 aatgttcatt ctaatcgact ccatttatta tcctggtaaa attcgatatg acgtatttcg
     4261 tcgtagtgaa ttttgtcttt tccaacgtag atccccagct catcactacc taagatcttg
     4321 ccaacaatat cttctggata gtatccttca gcgtttcgtt cttctttttg aatagatact
     4381 gatctatttt ttatgactgc agcgttcaat acttccgcaa tctcttcagt cgtcatttgt
     4441 tcttttgcta aattttgact ggttcgttgt tcattgtcgg catcaatttc ttcagtatga
     4501 tcagataaat aaaaccctgc ccactttaaa cgtccacgat cattgtaatc aatcattttt
     4561 aattccccca tttccgcccg catgaccacc aacaagtgtg cttctagtaa tgcttctagc
     4621 gctttctaac attgaagaag catgtacaat cgacgtaaaa ccatattttt gccgaatctt
     4681 gtcaataatt ttatctaaat tttcttcgtt aatttgcttt tgtggatcac taaataaatc
     4741 cagctgaagc gaatcggtgt acacaagctt gctataggtt attccaacgt gccttacttc
     4801 ttgtccttca taatacttat caaacaagaa aagacaataa ttctgcaatt cctttgtatt
     4861 atctgtgata ggtattttca tctggtgtga aaatcctggt ctcgtttcta agatcgacgt
     4921 accaatgttt aaatgaacac atcctgtttt acaattatgc tgcctgatac gaatggctac
     4981 ttgttccgcc atttctttta ccaccagctc gatttcattt cttctagtat aatctcgtgg
     5041 taacacttgt gaattaccat aagatttttc ctttgtcggt tctggcggga ttgctatatc
     5101 tgttcgatcg attccgttag catggaagta cagctgtaat ccaatcacac ctaatctatt
     5161 cttaatcgta taaggattcc agttagcaag gtctctaatg ctcaaaattc ccatttggtt
     5221 gagccttttt ttcattcgag aaccaattcc ccaaaagtcc gtcatttcgg gaatattcca
     5281 tactttctca ggaacgtttt cgtacgtcca ttccgcaata aatccttcat tatgctttgc
     5341 ttcattgtct aaggctaatt tagccaaaag gggattatcg ccgacgccta ccgtagcaat
     5401 caaacctaac tcttctttaa tacgttcttg gatcatttga gcgagcttct tccttcgctg
     5461 acttcgtgtt tcttcagtcg taaaaagatt cagtgatttt gtcactttta aaatcgattc
     5521 atcgatcgag taaatcagta gatcttcatc agccacatat cttctgaaaa tattatttac
     5581 ctgcatattt cgcttgatat ataggttcat acgtggtgga acaacgtgta gtgttttagg
     5641 aaatggttgt ggtaagtcac gtggtctact cacatttgta ataccatacc gcttttttgc
     5701 ttcaggagaa gaagctaata tcaatcctga accagtatta tcagatcgac tcataacaac
     5761 aagttctgtt gttaatggat ctaaatttct ttctatacat tcgacacttg cgtaaaagct
     5821 tttcatatcg attaaaaagt aatcatttac tggctctttt gaataatcca tcataaataa
     5881 cactccattc ttccacatta tacaaacata tgttcgatgt tgtaaagaag aaatagctaa
     5941 atacataaaa aaagaagcat aggtatgcta tcaccctgct tcttcttatg atttgtatga
     6001 tcaatttatt ctatcacttt actaattaat ttgaaaatct aataaaaact ctcactctaa
     6061 aagagtgaga gactgcaaat tagagtacgg acttttttgg agttccgttc tttaatatta
     6121 acaaatgaat aaaatcctta caacatttga taataagtta tcagtagaca cttacaaaaa
     6181 attctcatac tattgtcact tttctagata aatggcaaaa attttcagca ttaaatgaca
     6241 aaaactaatg agaatttgtc tttccatttt tctcctccat ttttgccaaa taacgataaa
     6301 tggtggttct tgagacgttc caccgttccg caatagtctt aataggtgta ccgctatcca
     6361 ctaaagtctt cagtaattcc aaatcttgtt cagacaattt ttcaggacga ccaccaaacc
     6421 gtcctcttgc acgagcagct gctcgtcctg ctgcggatcg ttccaggatc aaattacgct
     6481 caaattctgc aaatgccgca aacaaatgaa acatgagctg tcctgtagaa cttgatttgt
     6541 ccatcgtaat attttcttga atactatgaa aactcacacc acgctcattc agtcgattga
     6601 cgattgaaat caaatcttcc atatttctac ctaaacgatc taagcgccaa acgacaagtg
     6661 tatcgccaga gcgaacaaaa tcaatggcca tttctaatcc tggtcgatta ctttttgccc
     6721 cactcatatg atcagtaaaa attttttcac aaccaaattt atttaggctg tcttcttgta
     6781 aatccaaatt ttgtaaacca gtagataccc gggcgtatcc tattttcaaa aaaatcactc
     6841 cttcattaat tttattgtaa cataacttta tccatatagt ttaactgaac atagatattg
     6901 ttatgggttt atgaacatga aatgaattgt ttttttgaaa aataaagcaa aaaaaggaag
     6961 tgtccagaaa tgggtgattt tatgaacact ttcaattaga aaaaaagaat gtcgactaga
     7021 cactcttttt ttaaaatcat tatttttctt tttgatatgt tttttggtct cttttttttc
     7081 ttttttcttc agaatcttct agattagaaa ttgatttttg aattttttgt gtctcctgta
     7141 aagttcgctg aacttgtaaa gctgattctt ctttcggtaa attaatttta cttagaatat
     7201 cgctatcttg ataaaaattt ggtgtgttca caaattctgg cataaaaaat ccctactttc
     7261 tagttaaggt tcctgaataa gtcgcaatcc aattgtttcc agctgattta taactttgta
     7321 aatacaattt tccatagtac caataaccat ttcttttttc tttccaattc cgtgattgag
     7381 gaggtctaga aggccaacca actttatcaa aaatcatcgt tcctgtcact cgtatcgttt
     7441 tagaagctgc tcgagcaagt aatggagaaa ctatt
//
LOCUS       DADOZM010000128         7466 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.132, whole genome shotgun sequence.
ACCESSION   DADOZM010000128 DADOZM010000000
VERSION     DADOZM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.132"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..719)
                     /locus_tag="J8I47_002347"
     CDS             complement(<1..719)
                     /locus_tag="J8I47_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAZ6323770.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIK"
     gene            complement(719..1855)
                     /locus_tag="J8I47_002348"
     CDS             complement(719..1855)
                     /locus_tag="J8I47_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ6323771.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1887..3338)
                     /locus_tag="J8I47_002349"
     CDS             complement(1887..3338)
                     /locus_tag="J8I47_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAZ6323772.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3382..4146)
                     /locus_tag="J8I47_002350"
     CDS             complement(3382..4146)
                     /locus_tag="J8I47_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAZ6323773.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4174..4872)
                     /locus_tag="J8I47_002351"
     CDS             complement(4174..4872)
                     /locus_tag="J8I47_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAZ6323774.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4884..5663)
                     /locus_tag="J8I47_002352"
     CDS             complement(4884..5663)
                     /locus_tag="J8I47_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAZ6323775.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5679..6623)
                     /locus_tag="J8I47_002353"
     CDS             complement(5679..6623)
                     /locus_tag="J8I47_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6323776.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6669..7451)
                     /locus_tag="J8I47_002354"
     CDS             complement(6669..7451)
                     /locus_tag="J8I47_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323777.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEEKV
                     TSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATA
                     TYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2062 a         1600 c         1155 g         2649 t
ORIGIN      
        1 ttttttattt cttcttccat atcaccagtt ccaataatta ataatacgga attattctta
       61 atagcgtgaa attctttaaa aatttcaatc aaaaaacgat gatttttaga atatgaaatt
      121 cttccaatat gtcccattac aaacgtctga cttgaaattc ctaatttcat tcgtacatca
      181 tttctaatac tttcatcata ataaaatttt ttagcatcca ctgcatttgg aatcaaagtg
      241 aatttatcat ttcgaaacaa aaaatcacct gctacttttc cacatgaaaa ttcatgtgtc
      301 aaaacagacg ttaacttaca tcgataatat tgctttaaaa gatatttaaa atctctatct
      361 atcgatgtat tatggctatg tgctatccgt atcggaatcc cagctttttt tgcagctttt
      421 aaaggtaaat aactcatcgc atcaatatga gaatgaacaa tctcatattc tggatgatcc
      481 ttaaaaaatt gttccatcca tttaaaatat ttaggataat tttgtggata aagtctcggt
      541 gcataataga cctttcctcc aagacttatt atttcctcat cataatcact tttatatgga
      601 cgatgtgtca aaaaatcaaa ctgaatttta gttcgatcta tattcctata atagttcatt
      661 agcatcgtct ccaaccctgc acgatgcata tcattcacac actgtaatat tcgaatcatt
      721 atatatttcc cctatacaaa tttaatgttt cttgtacgat tttatcccaa ttatatttac
      781 tacaaataaa ctcagtagct tgattcttca aaactttaac catttccgat tgattgcatg
      841 cctcttctaa tcttatcttt aaatcactta catccgattt cttaaaaatc atagccttgt
      901 cttctactac ttccgtacac tctgagatat ttgaaactat acaacagttc ccataactca
      961 ttgcttctaa caaacttaat ggcattcctt ctaaatcact tggaagtgta tagatatacg
     1021 cattactata taattcttct aattcctgtc cttgtacaaa tccagtaaat ataattcgct
     1081 catcaccttt tgctaattcc tttagttcat ttgcaaattc atctgtatca gagcttcctc
     1141 cggctattat aagtttttta tctgtctgaa catctttaaa tgcctcaatt agataccgaa
     1201 ttcctttttc gggaaccaat cgccccaaaa acaaaatata agaatctttt gttaatccat
     1261 acttttctgt aatcaatcct gctgatttta tttcaatttt tttaacgcca tttggtataa
     1321 accttgtttt tcttccatat atatcttcaa aataattttg gacgctttca ctaagtacaa
     1381 ttatttcatc tgcaaatttt acagcacact tttcccctaa catgatgtat gtactagcaa
     1441 gcttgttcca tttttctctt tgatgatcta gaccatgaat tgttgcaaca catcttttcc
     1501 caaaaagttt tggcaaccac aacatagcac atggtccttc tgcatgaaaa tgaacaacat
     1561 catattttcc gaatgctgct ctaattccac caaatactga agaactcatt gctgcaattc
     1621 cttttatatt taatgtaaaa atagtcttta attttacacc tttatattct ttatttttat
     1681 gctggtcaaa ttcctttccg ctaacatgat gaccacttcg attatagcaa gtcacttcat
     1741 gcccaagtgc aaccattcgc gttgccaatt cttctaccac tatctcaatt ccaccttctc
     1801 ttgatggaat tcttttatga cctagcatac agatttttaa tttttttctt gccatcacgt
     1861 tttactcctt ctatagtatc tttatcttac atggaaccgt ttctatgaag cacagttttg
     1921 actgtcctaa acaaaattct aaaatctaat cccatactcc aattcctgat atattctgca
     1981 tcaagtttta caacctcttc gaagtctgtg atatcacttc taccactcac ctgccacatt
     2041 ccagtaatcc caggtttcat cgcaattctc gcacgatgat gaaaatcata cttctcatat
     2101 tctgacttca agcaaggtct aaaccccacc aggctcattt ttcccattag gacattataa
     2161 aattgtggaa actcatccaa tgatgtagat cgaataaatt gtccaattcc tgtcttcttt
     2221 gttccatcag gaagtatttt attgccaatg attcttgggt caaaatccat cttaaacatg
     2281 agattattac tcatcttatt ttctttcatc aactctgctt tgcgttcttc tgcatccata
     2341 tacatactac ggaacttata catcttaaat gtcttaccat tttgaccaat acgttcttgt
     2401 gcaaagaaaa tcggccctgg tgatttgatg taaataactg gtgctacgaa gataaaaata
     2461 acacctgttg caatacatcc aataattcca gcaagaatgt caatcgttct ctttataaat
     2521 gcttgcctca tcgtcataac attaatacta gatgtaataa cagtataatc accaactcgg
     2581 ccaataaatt gttttccacg tgtactatca gacacttttg taagattcat atgtacagca
     2641 agtcccattt ccgaacatct ggcgattaat tctttgggat atggaacact ttcgtcaaca
     2701 ttgatgaata cttcatctac ccaattctgt aaaagatatt ccgctgcatt atcagcattt
     2761 gcaactactg ggacacctcc gatggtagtt ccatacatat ctctgtctac tacaatcaca
     2821 ccactaagtt tatacatgcc ataattcttc tctttaagat tacgaactac attttttgca
     2881 atcttttgag atgttacaat aactaacgat acatcttcac cgtttttcat gcggtgatgc
     2941 aacaaccctt tccaaagtat acgaattata tatgttaata atccatacac cacccccatt
     3001 ataaataaag aaactctgga ataatcattt ccatcctgca ttgtaataag gtataaactg
     3061 cacacaagta acagcataaa agattgcttt atagaagcta cagcctcttt ataatatcct
     3121 ctttttaaaa catttttata tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct
     3181 ataaaaattg ccatgtggcg ataaatagga atcacatacg gattccagtc accctgtcgc
     3241 attacatatg atatataaaa agcaagctgg acacaaaata agtctaataa aataaaatca
     3301 atatgtttag accaaccact tgagtttttt ctatacatct ctcactctct cctcatttcc
     3361 tttttatcta cgaataaaga actaaaataa accaaacttc ttctttttta cctcttcata
     3421 gattggataa ttgatctcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac
     3481 tactttttcc ttaccaaact ccttggcaat ctgctcatag cactctttta agtaaaaggt
     3541 gcgcttagag accccgtgtg catcagaagc agccatctgc acgagattat gtttgaccat
     3601 tgttttggct gttttctgga tatctttgcc aaatacacca acataacttg gcgcagtaag
     3661 ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg
     3721 cgcgttgcgt tctggatgga cgatcactgg tatctttcct agttctaaca actcaaaaaa
     3781 aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc
     3841 atctaaatct gtaaataaaa tttcatctcg gtggatgtct tcgatgagcg ttccggtaat
     3901 acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg
     3961 taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg
     4021 gtgtggtgtg cataaaatat gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat
     4081 acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc
     4141 gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat aaccagcatc
     4201 tttgctgtgt tctgctccgt tgtatacgac acctaagact cgtgcttgta ccatatctaa
     4261 cagttctttt gctttagtca atgattcttt tcgtgacgta ttttcccgta cgaccaataa
     4321 ggtgccatcc gtttttgaag acatgatctg tgcatctgtt acggcaacga ctggcggcat
     4381 gtcgaagatc actacatcaa atagttgtct tgcttctgct aaaatctgat ccattcttgg
     4441 cgaacttagt agttctgatg gatttggtgg tttaggtcca cttggcagaa tcgacaaatt
     4501 gtcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa gaacagtact
     4561 taatccgctt gcgttgttca acttgaacgt tttatagatg acgggcttac gtaagtctgc
     4621 atcgactagc agcacccgtt gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt
     4681 ggtcgatttc ccttctccag gtcctgatga agtcacaaca atcgttttga tttgctgacc
     4741 agctgcggag gcaaactgga tattcgtccg aatcgtacga tattgttcag cgattggtga
     4801 agagggattg gtgagtgtga ccaaactgac tgcatgagtt tgggctgctt tttgtttctg
     4861 tgttcgtgcc attttctttc ctcctagact cttgagcggc ttctgcgtga tggcgctgtt
     4921 ttatcgtttg tagcttttgg ttgtgtctga gctggtcctt ttttacggat accagcattc
     4981 aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatctgtc
     5041 acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc cagacctaca
     5101 cctaccatca ttcctaacat taagccagtc attaaattca atttattatt tggcgaaaca
     5161 ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt catcacatct
     5221 tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc agccactgca
     5281 ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt aactgagatg
     5341 gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa acgatctttg
     5401 acttcattga gcactaaatc actgacgatc atatctttat acgtattgat catttgcagg
     5461 ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac gatcaattgt
     5521 gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag
     5581 ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc aaaaatttct
     5641 ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt
     5701 tgatctttta attctttttt cacacgatca agttcttgat cactgacttt ttgataggag
     5761 atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt accaaatgcc
     5821 tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt tttcatattc
     5881 gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt
     5941 ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc accatttggg
     6001 tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt aattttaccg
     6061 attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca
     6121 actgcatcaa cgagttcctt tagccctttc atattgatag agacatagtg atcgattggg
     6181 atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg
     6241 tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc acgaggaatg
     6301 ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat
     6361 cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa
     6421 gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat
     6481 tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca
     6541 attgctaata ctaatacgat aagaactgct aaaataatca gaaatatttt tttccctctt
     6601 gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcaacgt
     6661 tttaactgtc aatagcttat attgctatag cccatttgct cagcaacagt ttgtgctttt
     6721 gttgcgtatg ctgcgcttat tttttcattc cggatctgct ctatttgact gagcaccgtc
     6781 aaatattgat cgtaagtagc ggtggcagtc acttgaccat cctttaacat cgtatcgaat
     6841 aactgatcta gttgcgtgac ttgcgcgatc tgaatatcag cgaatcctag atattgcata
     6901 acaaccgttt tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca
     6961 tctgcacttg tcaccttttc ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     7021 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     7081 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     7141 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     7201 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     7261 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     7321 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     7381 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     7441 ggcttttcca tagattcact cctaag
//
LOCUS       DADOZM010000129         7433 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.133, whole genome shotgun sequence.
ACCESSION   DADOZM010000129 DADOZM010000000
VERSION     DADOZM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.133"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            392..1021
                     /locus_tag="J8I47_002355"
     CDS             392..1021
                     /locus_tag="J8I47_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323778.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1145..2254)
                     /gene="ugpC"
                     /locus_tag="J8I47_002356"
     CDS             complement(1145..2254)
                     /gene="ugpC"
                     /locus_tag="J8I47_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAZ6323779.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2463..2885
                     /gene="mgsA"
                     /locus_tag="J8I47_002357"
     CDS             2463..2885
                     /gene="mgsA"
                     /locus_tag="J8I47_002357"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAZ6323780.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3208..3744
                     /locus_tag="J8I47_002358"
     CDS             3208..3744
                     /locus_tag="J8I47_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAZ6323781.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3784..5547)
                     /locus_tag="J8I47_002359"
     CDS             complement(3784..5547)
                     /locus_tag="J8I47_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323782.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5550..7265)
                     /locus_tag="J8I47_002360"
     CDS             complement(5550..7265)
                     /locus_tag="J8I47_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323783.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2229 a         1419 c         1464 g         2321 t
ORIGIN      
        1 ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttgttaccg cagatgcaag
       61 cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg
      121 aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca
      181 agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct
      241 atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat
      301 ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt
      361 atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac
      421 gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat
      481 gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt
      541 tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac
      601 gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg
      661 gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg
      721 gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt
      781 agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt
      841 ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa
      901 tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc
      961 agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta
     1021 gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg
     1081 agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg
     1141 caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga
     1201 tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg
     1261 agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg
     1321 aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac
     1381 tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa
     1441 ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa
     1501 caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa
     1561 cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc
     1621 cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa
     1681 catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta
     1741 cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc
     1801 gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt
     1861 ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga
     1921 tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca
     1981 tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca
     2041 cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg
     2101 cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt
     2161 cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt
     2221 ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag
     2281 ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac
     2341 aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag
     2401 aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc
     2461 ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa
     2521 cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga
     2581 ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg
     2641 atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag
     2701 acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg
     2761 tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg
     2821 aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat
     2881 tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa
     2941 gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat
     3001 attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat
     3061 cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc
     3121 tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg
     3181 tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt
     3241 gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag
     3301 accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt
     3361 ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc
     3421 tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac
     3481 ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt
     3541 gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg
     3601 ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg
     3661 attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga
     3721 agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg
     3781 tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag
     3841 cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc
     3901 cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat
     3961 tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc
     4021 atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg
     4081 ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa
     4141 acgttctaca aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact
     4201 tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc
     4261 ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg
     4321 gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag
     4381 tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt
     4441 aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa
     4501 agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt
     4561 ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag
     4621 ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt
     4681 aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc
     4741 aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa
     4801 ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa
     4861 ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg
     4921 ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg
     4981 tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac
     5041 caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac
     5101 tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt
     5161 tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg
     5221 attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt
     5281 gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag
     5341 aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat
     5401 actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat
     5461 cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt
     5521 ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc
     5581 tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct
     5641 tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata
     5701 atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa
     5761 tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt
     5821 gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt
     5881 gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct
     5941 tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc
     6001 ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc
     6061 tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa
     6121 cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg
     6181 actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta
     6241 taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta
     6301 ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct
     6361 gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc
     6421 atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt
     6481 actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta
     6541 gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt
     6601 gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga
     6661 acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt
     6721 tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat
     6781 aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg
     6841 atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc
     6901 tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg
     6961 ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct
     7021 tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac
     7081 atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca
     7141 ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat
     7201 ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga
     7261 atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat
     7321 tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg aaagttttat
     7381 tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa aat
//
LOCUS       DADOZM010000130         7431 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.134, whole genome shotgun sequence.
ACCESSION   DADOZM010000130 DADOZM010000000
VERSION     DADOZM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.134"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="J8I47_002361"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="J8I47_002361"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAZ6323784.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3170)
                     /locus_tag="J8I47_002362"
     CDS             complement(1929..3170)
                     /locus_tag="J8I47_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAZ6323785.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="J8I47_002363"
     CDS             complement(3188..4111)
                     /locus_tag="J8I47_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAZ6323786.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="J8I47_002364"
     CDS             complement(4332..6824)
                     /locus_tag="J8I47_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAZ6323787.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="J8I47_002365"
     CDS             complement(6831..7298)
                     /locus_tag="J8I47_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAZ6323788.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DADOZM010000131         7008 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.135, whole genome shotgun sequence.
ACCESSION   DADOZM010000131 DADOZM010000000
VERSION     DADOZM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.135"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            264..860
                     /locus_tag="J8I47_002366"
     CDS             264..860
                     /locus_tag="J8I47_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ6323789.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(918..1400)
                     /locus_tag="J8I47_002367"
     CDS             complement(918..1400)
                     /locus_tag="J8I47_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323790.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1506..1703)
                     /locus_tag="J8I47_002368"
     CDS             complement(1506..1703)
                     /locus_tag="J8I47_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323791.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1998..2756
                     /locus_tag="J8I47_002369"
     CDS             1998..2756
                     /locus_tag="J8I47_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAZ6323792.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2816..3244
                     /locus_tag="J8I47_002370"
     CDS             2816..3244
                     /locus_tag="J8I47_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323793.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3290..3362
                     /locus_tag="J8I47_002371"
     tRNA            3290..3362
                     /locus_tag="J8I47_002371"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3323..3325,aa:Gln,seq:ctg)
     gene            complement(3512..3883)
                     /locus_tag="J8I47_002372"
     CDS             complement(3512..3883)
                     /locus_tag="J8I47_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ6323794.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4098..4283
                     /locus_tag="J8I47_002373"
     CDS             4098..4283
                     /locus_tag="J8I47_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323795.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4366..4554)
                     /locus_tag="J8I47_002374"
     CDS             complement(4366..4554)
                     /locus_tag="J8I47_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323796.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4578..4841)
                     /locus_tag="J8I47_002375"
     CDS             complement(4578..4841)
                     /locus_tag="J8I47_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323797.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4999..5256
                     /locus_tag="J8I47_002376"
     CDS             4999..5256
                     /locus_tag="J8I47_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAZ6323798.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5327..5518)
                     /locus_tag="J8I47_002377"
     CDS             complement(5327..5518)
                     /locus_tag="J8I47_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323799.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5659..5979)
                     /locus_tag="J8I47_002378"
     CDS             complement(5659..5979)
                     /locus_tag="J8I47_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323800.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6002..6436)
                     /locus_tag="J8I47_002379"
     CDS             complement(6002..6436)
                     /locus_tag="J8I47_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ6323801.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6771..>7008
                     /locus_tag="J8I47_002380"
     CDS             6771..>7008
                     /locus_tag="J8I47_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6323802.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTE"
BASE COUNT         2197 a         1372 c         1072 g         2367 t
ORIGIN      
        1 caatatcata aaaaaaagaa gatagagatt taataattat atatcagctg tttttttcaa
       61 ataagcaaaa attcctaaaa cacaacaatg aaagcgataa ctaagaaaat ccttacataa
      121 cgatttttct tattaaaatt gttttttgaa cttcgcttat tttttgtgag catatctgtt
      181 tacttacttt ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa atgcaaaacg
      241 caaatcaaag gagaaatttt attatgacaa cttttactga cactttgaaa aaccgccgtt
      301 caatctatca tctaggacgt aatgtttcac tttctaatga agaattaaca actttgatca
      361 aagaagcaat taaagaaagc ccaactgctt tcaatgctca atcaacacgc gcagttatcc
      421 ttttcggaga tgcacatgaa aaactttggg aaatcactga agaagcactt cgcccattga
      481 ctccagcaga agctttccca aatacacaaa acaaattagc tggtttcaaa aatggttatg
      541 gtacagtatt attcttcaaa gatactgacg tggtaaaagg tctacaagaa caatttgaat
      601 tatatgcaga caacttccca gattggtcag aacaatcaaa cggaatcgca acagctaata
      661 cttgggtagc attagttgat aaaggattgg gcgcaaactt gcaacattac aacccagtta
      721 ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa ctggaaacta cgttcgcaat
      781 tagtattcgg ttcaccagaa acaccggctg gggaaaaaga atacatgaac gatgccgatc
      841 gcttccgcgt atttggataa ttttatttga aataaaaaac agaggttgtg acaaacatct
      901 tgtcgcaacc tctgttttta ttctggatca atcataatat caatctctgc ctcatcgttt
      961 aagcgttggg acaaaaatgg aactaattct tctaatgatg cagaaaattt cagcatcggt
     1021 cctcgccatc cgtttcggaa aaaattcatt tttacgattt tagaaccttt tggctgatat
     1081 aatacaattt tattgatctc agaaaaagga acaccttttt tgtcaaagct attcaaaaca
     1141 agtccttctt ctgtgattcc tctactgtcc aacaaaaaac taataaaaat cattgctgac
     1201 aagatccctt ttgccaattc atctgctgta ggatgactga tgagtgtata ccaaatgact
     1261 agtgacacta agaagggtat caccaagtag acccatcgat ttcgcacagt ccgataaata
     1321 atttcttttc gaaccagcca aagaaataaa aacatcacaa caacgaataa aaacaataga
     1381 accgtaaaga atgttaccat gacaactcgc ctcgcttttt cttctccaga aaataggaaa
     1441 agaatactga tttaccttct caaagaaatt ggacttgcag cacgccgcac gtccaattgt
     1501 tttctttatt caaatgtatc atattttgca acaaccatgc ttgctttttc atctaataat
     1561 tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt catttttgat
     1621 acaacttcac caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata ttgttctcct
     1681 aaattaatca tttcttccgt catcaaaatc ctcctaaact gctggaactt ttctccacat
     1741 taccactgct atttctttgg agatttaact cattgatttt tatttacagt taaaaatcaa
     1801 ccttacaggc attatagcgt actgttttcg ttttcacaat taaaacagct gttgtattta
     1861 gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa actaattatt tcctacatat
     1921 taaaatataa tacttccatt tccaataaaa atacatttat aataggaaag ataaacagct
     1981 gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa actattgttt ctagggggca
     2041 cagctgctct tttattaagc ggtacaagtt attacctaca tatccattct ccagaacata
     2101 caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa aaagacaaaa gagaaagcag
     2161 aagttttact agatgttcca ttagagagcc agtttgattc tccttcttta gaaaatggct
     2221 gtgaagtcac ttcgctttct atgcttttat ctttttatgg ttatgaaaca actaaaaacc
     2281 agttagctga gcaattggat tacgtccctg ttttcaatgc agatgggact catggcgatc
     2341 ctaatgaagg atttgttggt gatatcagtg gcggagactg ggctatggtc gtctatgttc
     2401 ccccagttgc ctcccttgct cagcaaatcg tccaaacgga ttatcacact tttccaaaaa
     2461 ccgatgctca actcgatgat atcaaaaaag cactatctaa aggaaatcct gtctggactt
     2521 ctgtgactat caattttgaa gtcccagatg aaactgattt tatgacttgg accacaaata
     2581 atggagaagt aagagtcact cctcttgttc atgcttgtgt agtcactgga tatgacaaag
     2641 aaaatattta tgtcaacgat ccctatggtg taaaaaatcg agcagtaccg atcaacgatt
     2701 tttcggcgat tttcactgca atgggtggac agatgctaac tttggagaaa agctagtaat
     2761 tcaaacaaaa atcccttata ctcaagatac caaacctttg aagggagcgt agataatgga
     2821 aaaattcaca cataaaaaaa tggatccaaa tgaaattccg attatttttg tccgtgactg
     2881 caagggaaat gttcaaggta aagtctctat taatgagtgg aacgagcgtc gcagacctgc
     2941 taccctcaat gaactggaaa tcaaattgta tcgtcaatct cttgtttatt acgcagatca
     3001 agagtacgag aaagccaccg atttattgaa atttctaatt gctcgaacag agtatactcg
     3061 ctttgaatat attgaacgtt tagcaaacat ctatcatatt atgaatgaac ctgtcaaaga
     3121 atatcagtta ttggacaccg tcttgtcagt agcagaactg attgcccttc ctgctggatt
     3181 agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa caactatccg atcaagaaaa
     3241 ataacttgcg tttttttagg aaagtgctat gattattttg ttgaataaat gggatgtagc
     3301 caaactggta aggcacttga ctctgaatca agcaattgta ggttcgaacc ctgccatccc
     3361 aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa gaacaatctt tttgcagggg
     3421 gattattgta ttatttttac tacttgtatt tagtttacat aattaaatta tgaataataa
     3481 aatcgggcgt attcttaata caaaaactga tttacccaat aattgctaat gctttctccg
     3541 gaactttttc tctaggaact tcctcatacg tttctatgtg tgctccttta ttgctaattt
     3601 tcaaataatg attttctttt aacacagaaa gaccattaaa ttcaaccgtt cgttctttcc
     3661 cattggcatc agcagctttc tgcacatacc ttgctgtacc atattcattt acttcctctg
     3721 gtttttgtgt ctttacatag acctctcctt tttcaactaa tggatttagc tgatcaagta
     3781 taccagggat ttctccataa cttccttcag tataatattt cagtccaaat agcaaaatac
     3841 cactaatcgc cgccagtcca atcaaatatt taatcacttt catctttcta cctccttgtt
     3901 atatattcag tatatgggtc agttaaagaa attccatccg tcccaagttg gagatttttc
     3961 tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa aatgatcgtt atttgatatt
     4021 attgacgtgt ggataaatca gtttttctcg ttttgtccat gtaaaaaatc atgttgatag
     4081 caaaggatgt gttcaaaatg aatcaagaag aattatttca aaaagtcaag gaaatgatta
     4141 agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga acataaagaa caattaggac
     4201 cttataaaga gaaagcccaa aatcttttaa aagatgtaaa tatcgacagt gtcaaaaaca
     4261 aatttaaaaa tctattcaaa taagtaaaac tttcactttt gtaaaatcaa aggaggacaa
     4321 tgctgctgca tcgtcctcct ttggttcttt tctgaatact tcatactaaa ataaacgtgt
     4381 cggatcgtaa tgataagtca cattgccgtt cagtgaggct tttcccacca catatttacg
     4441 attctccaac ttcagccacg cttctcgaaa agccattaat tcttctcttt ttacatcaat
     4501 cttttcgatt tttccatcga tcaaatcttg aataagttgt tcatatggat tcattttttt
     4561 cctccatctg ctgtcaatca ataagttgta aaaataggtt catatgcttt tttggtatct
     4621 agatcttctc tgactttgac tgtcgtataa ataatttttt ctactgggat actataataa
     4681 cttccttcac cattattgaa tcggtgaacg tctttctttg ctaaaatacc atttatttta
     4741 ttcgtcagta cacgttgttc gacactgtta cttacaacat agtaaatcac ccgaatatta
     4801 ttgatcaaat gcaaatcgat ttctaacact acatgatcca tgaaaatccc actccttctt
     4861 tcactttgat tatagcaagt ttctcctcta atttcaaaat cgatacagat tttaccggat
     4921 aaatccacaa aactgcttgc gccatttcgc acaaagtgtt attttgtatc atggacttta
     4981 tttatcggag gcaatagtat gatcgcgatc ggtcaatttg ttttttatat tccttttttt
     5041 attatgataa gcattctttt ctattacata aaatggacaa aaaagaagtt ttcagtttta
     5101 ctggcctctt tacctgctgt atattttact tatcaaattt tttcttttag gcattgggaa
     5161 acaacgtctg tattaataac acacatcatc gaattgacgc tttcagtaat ttttcttatt
     5221 atttggatct attttttata taagaatcag aattgaatcc ctcgcatgtt taaaaagaaa
     5281 acgattaaag atgcagcaaa tttctgccga tctttaatcg ttttttctat tctgttatac
     5341 caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt taattctata aattcttgtg
     5401 cgatcgggta cttttcttcc aaattttctt tgttcttttc gatcaggctt ttcgcttctt
     5461 catatttttt atctgcacat agtgctttta cttcctgtaa tacttttcct gcttccattt
     5521 gcctcaccct cttgccattt tattgcttta agttttattt aagttatacg cattattcta
     5581 gtttcatccc aaataacctt ttttcatttt tactcctcga actaatcagt tttctaaagg
     5641 ctgattggtt cgtctttttt atttttcctc catttcattc tgctcttctg ttaatttatc
     5701 taatgctttg attgatgctg cggctcttgc ataatcatag ctgtataatt ccataaattt
     5761 atcaggcaga acaaaccacg ctggatgata accgttagct gtgacaggga atcctttatc
     5821 ttgttggtta tctccatccc atctcagagc aacacctttt tttcctttcc actttactaa
     5881 agcgatcgag tatcccttct ttgtttgtgt gccgtcttca ataattgcta aaatttctat
     5941 tttttctttt ggcgcacgga cttcttgagg acgaaccatt ttctattcct cccttatctc
     6001 attacttcac gaccaacaca ctgcaagggg catgtgatac gacataagaa gcggtcgatc
     6061 caactaatgc ttgttgtata gcaccttttc ctgtcgctcc cataacgatc aaatcaatat
     6121 tttcttgtcc agggatgaca ttggcaatca aatttcttgg atctccagtt tcaactattg
     6181 cgtggatctc agcaatgccg tactctttgg catcattgat ttttttcagc atggctactt
     6241 ctgttctttg tttttcatct gcatagattt ttgaaaaggc ataggcacta tgggtcagct
     6301 caactttgtt gatgattgac acgataaata agtgggcttc attgtccttt gctaagtcaa
     6361 gggcttcagc gaacgctttg atcgattgtc ttgatccgtc aactgctacc atgatgttct
     6421 ggtatctttt actcattgga accgcctcct ttttctttta ttgtaacatc tcaagcaaaa
     6481 atacaaaaaa tatacgcaat atgtaaataa aatgatgaac aaaagcaatt tctttcaagt
     6541 tatttataca tttcttttct catcagaaca cctgttctaa tcccattatt tctgcattat
     6601 gtgaaattcc tcatcatttt tcacatcgag cgaacatcat tctgttacaa aaaacgtgga
     6661 tttgtcataa attgtcatgc ttcttttacc tatgaaatat tttcacgtgt taaagtattc
     6721 ctcgttgaac acaacaaata aaacttttta acgaataaag gagatttttc atgacatcac
     6781 ttaaaacttt actttttgga acaactttgg ctgccggcgc tgcattcttc atgggaacga
     6841 ctgctcatgc ggacgaagct tataccgttc aatcaggtga tactctatca acgatttctc
     6901 aaaaatacgt tggtgataac tccttgatca atgccattgc agaatcaaat tcaatttctg
     6961 atattaacct gatctactca ggacaacaat taacaatccc aacagaag
//
LOCUS       DADOZM010000132         6924 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.136, whole genome shotgun sequence.
ACCESSION   DADOZM010000132 DADOZM010000000
VERSION     DADOZM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.136"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            94..1248
                     /locus_tag="J8I47_002381"
     CDS             94..1248
                     /locus_tag="J8I47_002381"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAZ6323803.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="J8I47_002382"
     CDS             complement(1270..2022)
                     /locus_tag="J8I47_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6323804.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="J8I47_002383"
     CDS             complement(2386..5052)
                     /locus_tag="J8I47_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ6323805.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="J8I47_002384"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="J8I47_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAZ6323806.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2043 a         1421 c         1252 g         2208 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DADOZM010000133         6911 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.137, whole genome shotgun sequence.
ACCESSION   DADOZM010000133 DADOZM010000000
VERSION     DADOZM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.137"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            46..936
                     /locus_tag="J8I47_002385"
     CDS             46..936
                     /locus_tag="J8I47_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAZ6323807.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1009..1833)
                     /gene="nadE"
                     /locus_tag="J8I47_002386"
     CDS             complement(1009..1833)
                     /gene="nadE"
                     /locus_tag="J8I47_002386"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAZ6323808.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1845..3317)
                     /locus_tag="J8I47_002387"
     CDS             complement(1845..3317)
                     /locus_tag="J8I47_002387"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAZ6323809.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3954..4316)
                     /locus_tag="J8I47_002388"
     CDS             complement(3954..4316)
                     /locus_tag="J8I47_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAZ6323810.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4539..5291
                     /locus_tag="J8I47_002389"
     CDS             4539..5291
                     /locus_tag="J8I47_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ6323811.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5431..6354)
                     /locus_tag="J8I47_002390"
     CDS             complement(5431..6354)
                     /locus_tag="J8I47_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ6323812.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6369..6641)
                     /locus_tag="J8I47_002391"
     CDS             complement(6369..6641)
                     /locus_tag="J8I47_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323813.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2136 a         1363 c         1171 g         2241 t
ORIGIN      
        1 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
       61 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
      121 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
      181 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
      241 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
      301 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
      361 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
      421 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
      481 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
      541 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
      601 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
      661 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
      721 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
      781 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
      841 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
      901 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
      961 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
     1021 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
     1081 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
     1141 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
     1201 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
     1261 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
     1321 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
     1381 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
     1441 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
     1501 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
     1561 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
     1621 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
     1681 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
     1741 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
     1801 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
     1861 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
     1921 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
     1981 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
     2041 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
     2101 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
     2161 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
     2221 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
     2281 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
     2341 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
     2401 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
     2461 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
     2521 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
     2581 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
     2641 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
     2701 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
     2761 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
     2821 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
     2881 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
     2941 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
     3001 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
     3061 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
     3121 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
     3181 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
     3241 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
     3301 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
     3361 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
     3421 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
     3481 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
     3541 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
     3601 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
     3661 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
     3721 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
     3781 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
     3841 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
     3901 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
     3961 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
     4021 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
     4081 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
     4141 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
     4201 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
     4261 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
     4321 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
     4381 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
     4441 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
     4501 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
     4561 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
     4621 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
     4681 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
     4741 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
     4801 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
     4861 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
     4921 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
     4981 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
     5041 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
     5101 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
     5161 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
     5221 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
     5281 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
     5341 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
     5401 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
     5461 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
     5521 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
     5581 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
     5641 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
     5701 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
     5761 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
     5821 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
     5881 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
     5941 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
     6001 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
     6061 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
     6121 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
     6181 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
     6241 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
     6301 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
     6361 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
     6421 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
     6481 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
     6541 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
     6601 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
     6661 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
     6721 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
     6781 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
     6841 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
     6901 aagtaaagaa c
//
LOCUS       DADOZM010000134         6881 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.138, whole genome shotgun sequence.
ACCESSION   DADOZM010000134 DADOZM010000000
VERSION     DADOZM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.138"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(1..1096)
                     /locus_tag="J8I47_002392"
                     /pseudo
     CDS             complement(1..1096)
                     /locus_tag="J8I47_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1339..1794
                     /gene="tnpA"
                     /locus_tag="J8I47_002393"
     CDS             1339..1794
                     /gene="tnpA"
                     /locus_tag="J8I47_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAZ6323814.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2059..2157
                     /locus_tag="J8I47_002394"
                     /pseudo
     CDS             2059..2157
                     /locus_tag="J8I47_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2380..2610)
                     /locus_tag="J8I47_002395"
     CDS             complement(2380..2610)
                     /locus_tag="J8I47_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAZ6323815.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2840..3658)
                     /locus_tag="J8I47_002396"
     CDS             complement(2840..3658)
                     /locus_tag="J8I47_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ6323816.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3819..4508
                     /locus_tag="J8I47_002397"
     CDS             3819..4508
                     /locus_tag="J8I47_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAZ6323817.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4522..6024
                     /locus_tag="J8I47_002398"
     CDS             4522..6024
                     /locus_tag="J8I47_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323818.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6037..6513
                     /locus_tag="J8I47_002399"
     CDS             6037..6513
                     /locus_tag="J8I47_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ6323819.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6610..6720)
                     /locus_tag="J8I47_002400"
                     /pseudo
     CDS             complement(6610..6720)
                     /locus_tag="J8I47_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2237 a         1082 c         1249 g         2313 t
ORIGIN      
        1 atagcttcct ccaaaccttc cattaccgct aatgcattgg gcttttcaga gatacgatag
       61 gataaaatct cactattgaa catatccata aaaggatcta gatacaattt cttttgacgg
      121 ataatgcctt tggcatccac ttcatggtat ttaaattcag ttgtatccgt cgtgattttc
      181 tgatggcaga tactggtgta gaaacgacga tggatccgat ttttggctac tgctccgact
      241 tgtcctcgat aagaattgta gcgccgtgat ttacgtgtgt atgtcgttac ttttattcct
      301 agcttcttga tcaggcgctg aactttcttt ttattgatag agaaaccgcg gtttcttagt
      361 tcattggtca tccgtaaaca gccataatcc ttgtgcttct cccgaacttt aagcatttcc
      421 gcttcaatct cttgatctgg atttttacgg tccaatcgtt tttgccagta cataaagatt
      481 gccttaggga actttaaagt ttctagaagc tcaactaatt taaacggtcc tcggaggctt
      541 gcgatgattc gtgcgattgc ttcattcgtc gttgttgggc ttcctgtttt cgcagcttcc
      601 tcaattcttt taaaaaggca ttttcaattt gaagggaacg gactcgtttc tccaactcct
      661 cgatgcgttc acgttcttta gacgttgctt tagttgtaga ttttttctta tttttgggtt
      721 catttttctt agacaagata ggtggacatc ctttcgtttt tgagagtcca tcgatcccac
      781 cctcacggaa ttgacgcatc caactagcga tcaaattagg gttgttcatg ttaagtgtgt
      841 tggcaacttc acgataggac agctcacttg ttagatataa ctctatcgca tccagcttaa
      901 attgaacaga atattttttc ttttgtcgct ttcttactag accctcttca ccaaattctt
      961 gataggcatt gatccagcgc ttcacttgtg atggagcctt aatggaatat ttctttgcca
     1021 gataatcact tccgccttta ccatccaagt aatcatgaac gatctttaat ttaagttcaa
     1081 aagtatactt agccataaaa aacaccgacc tccaaaagtt agattttttg gtctaacttt
     1141 tgggggtcgg tacaaaactt aaaagtttta gaccctttta ttttcttaat gacttcagtc
     1201 agttttgtgc gtaaagcgtg cgaaacaaga aaaccgcgcg tttctacgcg tggaagaagt
     1261 atctttagcg ataaaaactc ctttcgatat actagtgagt ggctaccaac cgctcatcag
     1321 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcatatac tcgttggaat
     1381 tgtaagtatc atattatttt tacaccaaaa tatcaacgaa aagtaatcta tgggcaatta
     1441 cgaaaagatc ttggaaaaat acttaggaag ttgtgcgaaa tgaaagatgt ggaaatcata
     1501 gaagcacatg caatgctaga tcatattcac atgcttgttc gagtaccacc aaaaatatgt
     1561 atatccagtt ttatggggta tctgaaaggt cgtagtgcgg tactaatcca cgagcagcac
     1621 gcaaatctca aatataaata cggaaacaaa tccttttggt cgaagagata ctatgtgagt
     1681 acagtcggat tgaatcaaaa gacaaatgaa aaatacatcc gtgaacaaga agctgatgat
     1741 cgcattcgat atattataag taaaagagag tacgtagaac catttaaaaa ataaagagaa
     1801 gtaagtgagt gaagatgagc agttggcggt cttttaaaaa tcccctttta aggggcagcg
     1861 agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg tatgggttac
     1921 tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg atgaaatttt
     1981 ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa cacaaattca
     2041 aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga ttatcgaaca
     2101 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
     2161 ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata ggcagatgaa
     2221 ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa aaggatcata
     2281 aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt tgtctcataa
     2341 cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa cgttgttctt
     2401 ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa caccctttat
     2461 agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt tagattcagt
     2521 aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa taaatttatt
     2581 taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa tgagctacac
     2641 gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga taaaatcaag
     2701 aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag taaaaaataa
     2761 gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa accgttaggt
     2821 ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc aaaacttttt
     2881 ctcttaattc aactgaatca atattattgt tttgttcata accatgaact aatagatcac
     2941 aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat tcttcaatgg
     3001 ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag ttagttatag
     3061 caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta tctttcgttt
     3121 tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa gcgacttggg
     3181 cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga aacatatttt
     3241 ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg ttttttgatt
     3301 gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta atagtggaat
     3361 agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta gtcggatgtt
     3421 cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa aaaccagaaa
     3481 aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg tttttcttaa
     3541 tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa tcagcgactt
     3601 ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata taactcatac
     3661 aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa aaaaactcct
     3721 aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt tttggagttg
     3781 ttatccaaaa ataataaaaa attggagtgt ttaggataat ggataaaaag aggtttttag
     3841 atcttatttc aaaaggtttg attgtttcct gtcaagcatt acctggtgaa ccattttaca
     3901 aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct ggagcagttg
     3961 gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta tcattaccaa
     4021 tgattggaat tattaaaaga gattatccac cagaatctcc attcattact gccactatga
     4081 gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat tgcactctta
     4141 gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa gaaaaatatc
     4201 ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat gcatataaag
     4261 caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt aaaaaacaag
     4321 atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt gtaattgctg
     4381 agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga gttgcaggta
     4441 ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt gttaaagcat
     4501 taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg cgctttcatg
     4561 ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg agcgttaaca
     4621 aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca tacaatttta
     4681 agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat ttttgcaatt
     4741 ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc tggtggagta
     4801 gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc agcaaaagat
     4861 gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt tgcattttta
     4921 cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg aggatcaaga
     4981 tttattccga ttgtttctag tttagctgca atcattattg gttctttctt ttatttaatt
     5041 tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca aatgggtagt
     5101 ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg actacaccac
     5161 accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt agctggatca
     5221 acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa ccacactggt
     5281 ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat gtttggatta
     5341 ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa aaagaatggt
     5401 gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga accagttgaa
     5461 tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt agatggatta
     5521 tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc aggtggtttg
     5581 atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg gttaaaatta
     5641 attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt ctttatcaaa
     5701 aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc tgaaaaagta
     5761 ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc tcttggtgga
     5821 aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc tgttacgaat
     5881 ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt cttcgaagtg
     5941 aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca agaaatcaat
     6001 caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt caaaaagaaa
     6061 aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac agaagtaaat
     6121 gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc tgatggttca
     6181 aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa acatgctatt
     6241 aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga tacagttgaa
     6301 ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac tcctgaaacc
     6361 tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac ttctgttata
     6421 gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg tcgaattgct
     6481 catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa gctataaaat
     6541 aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt tttctttctg
     6601 ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc gtaatttaaa
     6661 gttataaaag cttcgaaatc cattggcatt tcgttggaat accttgatat gattattcaa
     6721 atataaaata tagagagaag agagagaggg aagatattta taaagggcaa agttatgttt
     6781 actcaactta ggaaatagaa atagatcaat ttatattctc ttgtcaatcg aatatgaacc
     6841 ttagcactat ataccaaata tagtaaaatg ctgaaagatg t
//
LOCUS       DADOZM010000135         6769 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.139, whole genome shotgun sequence.
ACCESSION   DADOZM010000135 DADOZM010000000
VERSION     DADOZM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.139"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(291..1307)
                     /gene="tsaD"
                     /locus_tag="J8I47_002401"
     CDS             complement(291..1307)
                     /gene="tsaD"
                     /locus_tag="J8I47_002401"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAZ6323820.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1304..1768)
                     /gene="rimI"
                     /locus_tag="J8I47_002402"
     CDS             complement(1304..1768)
                     /gene="rimI"
                     /locus_tag="J8I47_002402"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAZ6323821.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1775..2317)
                     /gene="rimI"
                     /locus_tag="J8I47_002403"
     CDS             complement(1775..2317)
                     /gene="rimI"
                     /locus_tag="J8I47_002403"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAZ6323822.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2301..3125)
                     /gene="tsaB"
                     /locus_tag="J8I47_002404"
     CDS             complement(2301..3125)
                     /gene="tsaB"
                     /locus_tag="J8I47_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAZ6323823.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3214..4194)
                     /locus_tag="J8I47_002405"
     CDS             complement(3214..4194)
                     /locus_tag="J8I47_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ6323824.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4218..5702)
                     /gene="galT"
                     /locus_tag="J8I47_002406"
     CDS             complement(4218..5702)
                     /gene="galT"
                     /locus_tag="J8I47_002406"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAZ6323825.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5714..6703)
                     /gene="galE"
                     /locus_tag="J8I47_002407"
     CDS             complement(5714..6703)
                     /gene="galE"
                     /locus_tag="J8I47_002407"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAZ6323826.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1811 a         1500 c         1205 g         2253 t
ORIGIN      
        1 tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt
       61 acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga
      121 aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt
      181 tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt
      241 tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat
      301 aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc
      361 acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc
      421 actagttcta cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct
      481 tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct
      541 cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca
      601 tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt
      661 tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg
      721 taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga
      781 cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca
      841 atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca
      901 agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct
      961 gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca
     1021 gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat
     1081 aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt
     1141 tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt
     1201 atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc
     1261 gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg
     1321 tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc
     1381 gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata
     1441 gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta
     1501 acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct
     1561 cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta
     1621 aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag
     1681 gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt
     1741 catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa agttttatca
     1801 tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa
     1861 accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca
     1921 tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga
     1981 ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga
     2041 acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat
     2101 gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat
     2161 tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta
     2221 gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa
     2281 aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc
     2341 gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt
     2401 ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata
     2461 tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag
     2521 aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg
     2581 aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc
     2641 cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata
     2701 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
     2761 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
     2821 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
     2881 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
     2941 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
     3001 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
     3061 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
     3121 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
     3181 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
     3241 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
     3301 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
     3361 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
     3421 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
     3481 aaaaagcgca tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc
     3541 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
     3601 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
     3661 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
     3721 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
     3781 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
     3841 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
     3901 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
     3961 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
     4021 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
     4081 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
     4141 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
     4201 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
     4261 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
     4321 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
     4381 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
     4441 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
     4501 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
     4561 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
     4621 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
     4681 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
     4741 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
     4801 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
     4861 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
     4921 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
     4981 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
     5041 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
     5101 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
     5161 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
     5221 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
     5281 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
     5341 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
     5401 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
     5461 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
     5521 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
     5581 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
     5641 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
     5701 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
     5761 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
     5821 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
     5881 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
     5941 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
     6001 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
     6061 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
     6121 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
     6181 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
     6241 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
     6301 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
     6361 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
     6421 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
     6481 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
     6541 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
     6601 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
     6661 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
     6721 ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttt
//
LOCUS       DADOZM010000136         6767 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.140, whole genome shotgun sequence.
ACCESSION   DADOZM010000136 DADOZM010000000
VERSION     DADOZM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.140"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            80..1552
                     /locus_tag="J8I47_002408"
     CDS             80..1552
                     /locus_tag="J8I47_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6323827.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            1635..2423
                     /locus_tag="J8I47_002409"
     CDS             1635..2423
                     /locus_tag="J8I47_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAZ6323828.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(2490..3158)
                     /locus_tag="J8I47_002410"
     CDS             complement(2490..3158)
                     /locus_tag="J8I47_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAZ6323829.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            3273..4985
                     /locus_tag="J8I47_002411"
     CDS             3273..4985
                     /locus_tag="J8I47_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323830.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4988..6757
                     /locus_tag="J8I47_002412"
     CDS             4988..6757
                     /locus_tag="J8I47_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6323831.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
BASE COUNT         2065 a         1142 c         1472 g         2088 t
ORIGIN      
        1 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
       61 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
      121 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
      181 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
      241 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
      301 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
      361 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
      421 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
      481 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
      541 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
      601 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
      661 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
      721 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
      781 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
      841 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
      901 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
      961 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     1021 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     1081 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     1141 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     1201 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     1261 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     1321 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     1381 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     1441 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     1501 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     1561 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     1621 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     1681 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     1741 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     1801 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     1861 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     1921 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     1981 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     2041 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     2101 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     2161 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     2221 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     2281 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     2341 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     2401 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     2461 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     2521 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     2581 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     2641 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     2701 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     2761 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     2821 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     2881 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     2941 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tatttgcaag
     3001 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     3061 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     3121 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     3181 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     3241 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     3301 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     3361 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     3421 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     3481 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     3541 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     3601 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     3661 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     3721 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     3781 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     3841 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     3901 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     3961 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     4021 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     4081 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     4141 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     4201 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     4261 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     4321 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     4381 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     4441 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     4501 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     4561 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     4621 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     4681 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     4741 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     4801 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     4861 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     4921 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     4981 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     5041 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     5101 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     5161 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     5221 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     5281 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     5341 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     5401 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     5461 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     5521 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     5581 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     5641 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     5701 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     5761 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     5821 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     5881 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     5941 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     6001 aaactgtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     6061 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     6121 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     6181 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     6241 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     6301 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     6361 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     6421 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     6481 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     6541 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     6601 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     6661 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     6721 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtg
//
LOCUS       DADOZM010000137         6634 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.141, whole genome shotgun sequence.
ACCESSION   DADOZM010000137 DADOZM010000000
VERSION     DADOZM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.141"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            150..2825
                     /locus_tag="J8I47_002413"
     CDS             150..2825
                     /locus_tag="J8I47_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAZ6323832.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3007..3246
                     /locus_tag="J8I47_002414"
     CDS             3007..3246
                     /locus_tag="J8I47_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323833.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3262..4635
                     /locus_tag="J8I47_002415"
     CDS             3262..4635
                     /locus_tag="J8I47_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6323834.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4639..6273
                     /locus_tag="J8I47_002416"
     CDS             4639..6273
                     /locus_tag="J8I47_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6323835.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6436..>6634
                     /locus_tag="J8I47_002417"
     CDS             6436..>6634
                     /locus_tag="J8I47_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323836.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2324 a         1071 c         1332 g         1907 t
ORIGIN      
        1 agttgtgatt tccaaaatca caactttttt cttttgtcaa gattttgagt gggatatatt
       61 tttgtagaac tgaagatcat gaaattatga gaggttggag gtaatgtagt catacaaata
      121 aaaatttttt agaaagtgag tggtaattaa tgaaaatgaa tcacacttca attcagaagc
      181 atttacaact gttaaataca ctggggattt cttatgttaa tttgagaact ttttctacat
      241 taaacaagaa aaccgtttct catatttttt ctactgcaga ttctcaagca attattaact
      301 atttagaaaa acagaaattg tctgaaggta cagtaaacat aacttataat cctattaaac
      361 aagaggtgat tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt
      421 ttgtatttat tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga
      481 aaaaggcaga aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat
      541 catttgtaaa agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac
      601 caaataatca cgagacgatt cttactattc agaattttct tcaattactt caccgaagat
      661 ttggagtaga agatgaagtt gatattgata cttctgttta taatccttca agattatgca
      721 aattagttgg tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat
      781 ctagtttcct ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac
      841 agatagttga agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta
      901 gagaagtgtt accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat
      961 taacttatac cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat
     1021 gtccattaag aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata
     1081 ataagattcg atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg
     1141 taaaaaaata tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc
     1201 aagaatatta ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag
     1261 gtcaagaaga aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac
     1321 aaaaagtcat tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa
     1381 tagaaggtgg atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca
     1441 ttaaagctag aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc
     1501 caatcttttt tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc
     1561 agttacaaga ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc
     1621 aattaacaac taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag
     1681 gaacgaactt agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa
     1741 acgttacgaa ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa
     1801 cgggtaaaac cactacgtta cactttattg cctctttatt tggaaatcca actacggttc
     1861 ttagaaatat gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag
     1921 gtgttccact aatactagat gagttaggag cagcaactca aaataacttt agctcgttga
     1981 tctatcaatt agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc
     2041 cacaagagac tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt
     2101 tcaatgagaa attaactggt ttgaaagttc gatatatcga attttctgat atcaagttta
     2161 caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg
     2221 gtataaagtt atttcttttg aaactctttg aacaagggat tacaaaaatt gaagcaactt
     2281 ttgtcgaagc tcaaaaaaaa ctatgggctg ttttacctga acattctttg aagcaacgca
     2341 ttggtaataa tctagctttg atttatagtg gagccctcct aattcaagat atactgggct
     2401 atcctttacg attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg
     2461 tagatactag tttaggaagt gagcaaacgg tatttgaccg agtgaaaaca ttgttggtga
     2521 tgaatagcca taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg
     2581 gaaaagtatc aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg
     2641 aggacttgtt aaggcgagaa tttcaagtaa aggatctaac ctatcttttt aggcaattgc
     2701 ttgattcggg taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc
     2761 gtcaagttct tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat
     2821 tgtaatttag aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta
     2881 ttgatttagc aaaacaactg attacttatc tgtatgatag cgagcaaacc caaacggaag
     2941 taaatgaagc ttgatctatg cttcatttac ttcattcaat caaacatgaa agaaaggaaa
     3001 attctaatga ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa
     3061 catttttcaa atcaagcctt ttttacaaaa ccaatttact tggatttcga aataagaggg
     3121 gtatctgctg gatctatcgg gggtactctt caagcgttga agaacgaagg atatttagaa
     3181 aatcactttg tgcaacgttc gttcaataga agagtcgtga aaaaatggta tttagttcat
     3241 tcatagagag gggaatcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa
     3301 gaagcgaaaa ttagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa
     3361 tatgaacaaa ttccccaacg attttcagac aaaggagaat caggggaaaa agcggagcgt
     3421 ccagcttttc gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat
     3481 gtttggcgtt atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg
     3541 tgccaacaac atcaggttca agtgtattcg atttctgaac ctcttcctgc ttctttctca
     3601 cacgcgtcaa cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga
     3661 aacgtcatta ttgagaatgt tcgtgccgga ctttcctata aaaagcaaca acggcaatat
     3721 ttgtcttctg cggtaccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagta
     3781 cacgaagcaa ggattgttcg ctatattttt caactttatt tgaccaaaaa atatggctac
     3841 aaaaagcttt gtcaacgatt gacgcaacaa aagcttttct tccgcgaacg tccttttcag
     3901 ccctatcata tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga
     3961 tctttgggga agtatctagg aacctttgaa ccgattctct cgaaagcaac ctttttacaa
     4021 gcccaagaga ttcgccaatc aaggtgtaca gctaaaaaag atacgtatct gtacttatta
     4081 cgtcaaaaaa ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat
     4141 acaaagaaga cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatt
     4201 tttcttcggg cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa
     4261 tacgaaggcg tctatcaagc cttgattgcc caattaacgc aacaattagc cgaaaagcga
     4321 aaagatgaaa aacgcaaagt aatccaacaa aagaaacaag aagcacaatg gattaaggca
     4381 tttgaagagg gcaattgttc actggaagaa ctcaagcaac aactgcaagc tcttcaaaag
     4441 ccgatgtcta cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg
     4501 cttcaactaa aagacctctc tagtcgacaa ttattatgtg actatgtttg tcggattgag
     4561 gtaacaaagg ataaaacagt ttgtgggatt tacttccgac aactgacaca aaatatttta
     4621 gaaaggaacg aatgatctat ggaaatgata caaaccaaac gagtcgcatt gtatattcgc
     4681 gtgagtacac aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga
     4741 cgacagtttg ccgaacggat gggctatgaa gtggtagaag tttatgccga tgaaggaatc
     4801 agtgggaaat ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac
     4861 catcgttttg atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat
     4921 gtcttacaaa cagtcgagga attattgcga tatgacgtag gattacattc gatctcggaa
     4981 caatttgacg tgaccacttc gacaggtaaa ttgatgttac aactccttgg gagtttcggt
     5041 gaatttgaac gcaaccaaat tgcggagaat gttcaaatgt caatgcaaag cttagtgaaa
     5101 gataaaaagc gttatgcagg aggccgacgt ttaggctatg tcagtggtat tgacacgaat
     5161 ggtcaaaaac aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca
     5221 aaatatttag gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat
     5281 caaacagtca aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa
     5341 atttatgcag ggtatttaga atatgcgcgc tacatcaatt gggaaaccaa acgacggaag
     5401 gggaagaatc cgagaccgat tttagtgaaa ggaacccatg aaccgattat ttcggaagaa
     5461 gattatcaaa aagtacaaga acgccttgct ttagaaggaa aacaacccaa atggaaccat
     5521 acaggtgaaa atgtattgac aggcttgtta cgttgtccag aatgtggcgc accgatggca
     5581 gcgagtaatg tcacaaatac cctaaaggat ggcacaaaga aacggattcg atattattct
     5641 tgttcagtct ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa
     5701 caagcggaaa catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt
     5761 ccccgattgg tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa
     5821 gaactcgtgg tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa
     5881 gctgctttgg aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg
     5941 actgaaaaac gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa
     6001 ggagagccga ttcaagtcaa agatgtccaa cggatattga ctaatttgga tcgttttctt
     6061 gcccagagtg agaagaagca aatcaaagcc ttgtatcgta cctttataga gaaaataacc
     6121 tttgatccta accataagga gatgttggaa attacgatga aattcacgca tgccgtggta
     6181 caacaattga atgagcaata tcaaatagcc gtatccaaaa caaaggatac ggctattttt
     6241 gtgttaaaaa caccctttac gttaacgatc tagtctaggt tatatcaaac aacgttagag
     6301 gggcgctaac gcttctctag cgatttgaaa tcgttagaga agtagttagg gaggtcctta
     6361 tcataaatag ggatatttat taatgaatat ttgaaatata attgtatata agaattaaaa
     6421 agagtgggga tagatatgaa aaagatgggt aagtgtattt tatgtgaaaa atacactgaa
     6481 cttactaaag aacatgtacc acctaaaaaa tcagggaata ctggaggtaa aaagaccaga
     6541 actggtaatt tagatgattt ccttaagagc gattttacaa aaggtgattt tccgaagggg
     6601 attaaaagga aacctcaagg aaatgtttat tata
//
LOCUS       DADOZM010000138         6570 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.142, whole genome shotgun sequence.
ACCESSION   DADOZM010000138 DADOZM010000000
VERSION     DADOZM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.142"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            127..579
                     /locus_tag="J8I47_002418"
     CDS             127..579
                     /locus_tag="J8I47_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323837.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="J8I47_002419"
     CDS             593..1282
                     /locus_tag="J8I47_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323838.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="J8I47_002420"
     CDS             1407..3758
                     /locus_tag="J8I47_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAZ6323839.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="J8I47_002421"
     CDS             3838..4233
                     /locus_tag="J8I47_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323840.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="J8I47_002422"
     CDS             4558..5175
                     /locus_tag="J8I47_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6323841.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="J8I47_002423"
     CDS             5216..5515
                     /locus_tag="J8I47_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323842.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="J8I47_002424"
     CDS             5549..5716
                     /locus_tag="J8I47_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6323843.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="J8I47_002425"
     CDS             complement(5761..6366)
                     /locus_tag="J8I47_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6323844.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DADOZM010000139         6564 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.143, whole genome shotgun sequence.
ACCESSION   DADOZM010000139 DADOZM010000000
VERSION     DADOZM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.143"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(7..1164)
                     /locus_tag="J8I47_002426"
     CDS             complement(7..1164)
                     /locus_tag="J8I47_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323845.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="J8I47_002427"
     CDS             complement(1167..1523)
                     /locus_tag="J8I47_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323846.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="J8I47_002428"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="J8I47_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAZ6323847.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="J8I47_002429"
     CDS             complement(2991..3830)
                     /locus_tag="J8I47_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ6323848.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="J8I47_002430"
     CDS             4015..4902
                     /locus_tag="J8I47_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAZ6323849.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="J8I47_002431"
     CDS             complement(5486..6181)
                     /locus_tag="J8I47_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAZ6323850.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="J8I47_002432"
     CDS             complement(6165..6563)
                     /locus_tag="J8I47_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAZ6323851.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DADOZM010000140         6546 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.144, whole genome shotgun sequence.
ACCESSION   DADOZM010000140 DADOZM010000000
VERSION     DADOZM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.144"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            119..625
                     /locus_tag="J8I47_002433"
     CDS             119..625
                     /locus_tag="J8I47_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAZ6323852.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="J8I47_002434"
     CDS             642..1541
                     /locus_tag="J8I47_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAZ6323853.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="J8I47_002435"
     CDS             1628..2512
                     /locus_tag="J8I47_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAZ6323854.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="J8I47_002436"
     tRNA            complement(2704..2775)
                     /locus_tag="J8I47_002436"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="J8I47_002437"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="J8I47_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAZ6323855.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4372..5409
                     /locus_tag="J8I47_002438"
     CDS             4372..5409
                     /locus_tag="J8I47_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAZ6323856.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="J8I47_002439"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="J8I47_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAZ6323857.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT         2298 a         1195 c         1344 g         1709 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttatt
//
LOCUS       DADOZM010000141         6523 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.145, whole genome shotgun sequence.
ACCESSION   DADOZM010000141 DADOZM010000000
VERSION     DADOZM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.145"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..506
                     /locus_tag="J8I47_002440"
     CDS             <1..506
                     /locus_tag="J8I47_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAZ6323858.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGVTA"
     gene            551..877
                     /locus_tag="J8I47_002441"
                     /pseudo
     CDS             551..877
                     /locus_tag="J8I47_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
     gene            complement(1271..2278)
                     /locus_tag="J8I47_002442"
     CDS             complement(1271..2278)
                     /locus_tag="J8I47_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323859.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2745..4151
                     /gene="lacG"
                     /locus_tag="J8I47_002443"
     CDS             2745..4151
                     /gene="lacG"
                     /locus_tag="J8I47_002443"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAZ6323860.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            4174..4497
                     /locus_tag="J8I47_002444"
     CDS             4174..4497
                     /locus_tag="J8I47_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ6323861.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4508..6190
                     /locus_tag="J8I47_002445"
     CDS             4508..6190
                     /locus_tag="J8I47_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323862.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            6396..>6523
                     /locus_tag="J8I47_002446"
     CDS             6396..>6523
                     /locus_tag="J8I47_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6323863.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYS"
BASE COUNT         2083 a         1001 c         1321 g         2118 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggagtaaca gcctaagata tttggagttc agagagggcg cgtttgattt
      541 tcaaacttcg caacagaacc ttctaacaat tcctggggcc atgtcgccca cagagatatt
      601 caaaatgttt gagatgggtg ctgatattgt taaggtgttc cccgccaact gtcttggacc
      661 ggaatatttc aatcaagtgc aagccccttt aggaagttta cctttgatgg ctgtaggagg
      721 ggtagatcag actaatgcac aaaactattt gaacaatgga gcctcttatg taggaattgg
      781 ttcaaaattt tttgaaaaaa gtgctgttca ccaactaaat tacgagaggc taatggagtt
      841 ggctgaatct tttatagata gtttaagagt tgaatagaac ccatttgcgg aatacaactt
      901 gttttataat atggcgttat ctgtataaaa catagtatca cagtgaatga gattgaactg
      961 gtagattgat ttttcgacca gttctttttg cgcgctctga tgcttgatat attcccaacg
     1021 agataaaaaa tcccccaaga tactaagatg aagcgtttat ccaacctcat acttagataa
     1081 aaaggaggaa atgttctgaa tgataaacaa aaattagcgc gcacaatttg gcgatgaaaa
     1141 aatcatattg tatttacgtc aaaatatcac catcaaatta tttatggaaa acataatagg
     1201 cataaacccg attgctcggc tactatgtgg acacaattat agttttaaag ataagtttca
     1261 attacttatt ttacacctct tctttaggga taaaactttt cctaacggtc aattttgcgc
     1321 cgattaatgt tttctttgat atgtcccgcg aatataccag ttgatcactt aataaatcta
     1381 cacaggtttt agctatctct tcgatatcta tcttaaaagt tgtaagtggt ggtgcaacga
     1441 attttgcgat gtcgtcatca ttaatactta ttactgccat atctttaggg atgttaatac
     1501 ccaattcatg gcagccctga agaacgccgg cagctatagt gtcagatggt atgaaacatg
     1561 cctcgggcat atcttcattt aactcagctt tcattttaag agttaattga tacccttcgt
     1621 caacagtaaa tgatccttgg gagaaaatat acttttcatt gagtagtccg ctcttttcta
     1681 aatgatatcg gaaggcatat tcacggctat caatctcttc tttacccgtt tctaggttaa
     1741 agaaatttcc tccaataaat ccaatttttg tgaaaccttc attaataaag tagttaattg
     1801 cttgtctggc catttcgtgg aaatcaggtt ctacagtatc gaagtgttca ggtaatgggt
     1861 ttattcctcg gattaccccc ttataccctt tttgatttaa gtagtacaat tccttttttg
     1921 tcaattgtcc tattgcgata aatccagtaa tatcgtcagg aatgcttttt acaccatcgt
     1981 ttttagagat gaaaatcaaa tctagattag cttttttact ataatttttc aaatcatctt
     2041 tgacatgttg atagtagaca tcctttttta actgttcctc tgagatccaa aaaagaatgg
     2101 caatcttgta cagagtagtc ttaatttttg tcagatcata tcctaaagat ttggctgtaa
     2161 cgattatttt gtttttagta tcagatgtaa tagagagtga ctcatcacct ttaaaaagtc
     2221 ttgatatagt agccgttgag taaccagatt tttttgcaat atctctaata gtaaccattg
     2281 ttcatcctcc tagggatata ataatccttt tctttgttaa agtaaatcga aactgctctt
     2341 ttagtaactt aaattacttt ttctagcagg aaacgaaact ctgatatcag cagagagcct
     2401 aatccattaa cagagaagat tgaatcgata atggatagcc acttggagaa tgattagtag
     2461 gtgggattga ttttagtggg aatcagtccg ttttcttgtt tcgtgtaaat taaattagta
     2521 aaaagataaa tttattttac aaaaaaacgt ttacaaaacg gtttttgaat ggtatattta
     2581 ttttacatca agggatataa gtgcttgtat atcgcaataa aatagatatt cgaggtcggt
     2641 ttttcgttgt gtaaaaaaat ttacttatac atgttaaaat tatgctgagg tgatttatgg
     2701 attattggca tattgatcaa actaattttc aaggagagat taaaatgaat tataaaactt
     2761 taccagaaga ctttattatg ggaggagcta cggcagctta tcaagcagaa ggctctacaa
     2821 aagaaggtgg aaaagggcaa gtagcgtggg atactttcct tgtagaaaag ggacgctata
     2881 ctgctgatcc agctagtgat ttttataatc aatatccagt tgatgctgaa ttatgcaaaa
     2941 aatttaaaat tgatagtatt aggatgtcta ttgcatggag ccgtattttt ccaaaaggct
     3001 atggggaagt gaaccaaaca ggagtggaat attatcataa ggtatttgct gaattgaata
     3061 agcatggtat tattcctttt gtaactcttc atcattttga tacccctaaa gaactatttg
     3121 ataatggaga ttttttaaat cgagaaaatg ttgaacattt cgtaaactat gcacagttct
     3181 gtttcgatga attttctgag gttcattatt ggagtacatt taatgagatt tatccagttg
     3241 cttcaaatca atatttgatc ggatcgtttc ctccagaagt tcaatttgat atagagaaga
     3301 ctgtaacttg tttacacaac atgatgtatg cgcacgcaaa gacagtaaac atttttaaag
     3361 atggtggata taaaggggaa attggtgttg ttcactcgct agagacaaaa tatccagcaa
     3421 gtgacaaaga agaagataag catgctgcga aattagatga tgcgttatca attaagttct
     3481 tattagatgc tacttattta gggtattact cagatgaaac aatggcacat attaatgaga
     3541 ttttagcagc aaatgggcaa tctgcagata ttgattctaa tgattttaga gagatgaaaa
     3601 aagcagcaaa tcgaaatgat tttcttggta tcaataacta tggtagccat tgggttaaag
     3661 agtacaaagg agaaagcaca cttcactaca acactaccgg agaaaaaggt agccaagtct
     3721 attgcattaa agggattggc gagagaatcg tgcgtgagga tcttccgaaa accgattggg
     3781 attggatcat ttatccaaaa gggttatatg atttacttgt acgaatccaa gacgaatatc
     3841 caaattataa taaaatatac attactgaaa acgggctagg gtataaggat aattttgaag
     3901 atgggattat tctagatgat gatcgaatcg attatttacg ccagcatttt aatgcgattt
     3961 ccgatgctat caaaatggga gttaatgtaa aggggtattt catttggtca ttaatggatg
     4021 ttttctcttg ggttaatgga tacactaagc gttatggact attctatgta gatttcgata
     4081 cacaaaaaag atatccgaaa gagtctgcat attggtataa atttgttagc gaaacaaaaa
     4141 cgataatata aaaatagtta ggagggcaag gaaatggata ggaaagaatt gcaattatta
     4201 ggatttgaaa ttgttgctta tgcaggagat gccagaagtt cattattaac agtgttgaag
     4261 gaagtgagag aaggtaattt tgataatgtt gaacgattat tagcggatgc tgatgaaaat
     4321 ttaaccttag cgcataactc acaaacgaaa tgtttagctg aagaagcgtc agggaacgaa
     4381 atggaagtag gcgtaatttt tattcatggg caagaccatt taatgacaac gattctatta
     4441 agagaactta tcaatgattt tgttgaattg tacaaagaaa aatttgctaa agcataggag
     4501 aagaaaaatg aatacgatta ttaagcaaat tgagaaggga aaaccgtttt ttgaaaaaat
     4561 ctcacgtaat atttatttag gggcaattcg cgatggcttt ttaactgcta tgccagctat
     4621 tctattttca agtattttta ttatgattgc ggctgttcca gaagtattta acgtgacgtt
     4681 accagaggca ttctcgacgt ggctttggaa agtttatggt tattcaatgg gattagttgg
     4741 tcttttggtt gttgctacaa cagctcgatg tttagctaat tcaatgaacc gtcaattatc
     4801 tgattcaggt aagcagatta atgaagtttc tgttatgtta gcgtcaattt gtggttttgt
     4861 aatgatggca gctgatcaaa ttgatggtgg gatagctact gattatttag ggacaaaggg
     4921 attattagca gctttcgttt ctgcgtttgt tactgtaaat gtgtataaat tctgtgttaa
     4981 aagagatttg acgatccgta tgccaaaaga agttcccgga acaatcacac agatgtttag
     5041 agatattatc cccttttcaa ttgcagtatt tattgccgtt ttgattgata cgggagttcg
     5101 tcaatttttt ggaacatcat ttgcagcagg tgtaattcaa ttattgcagc cattatttag
     5161 tgctgctgat ggatatgtcg gtattgcaat catttgggga gccatggcaa tgttctggtt
     5221 tgttggtgtt catggaccat cgattgttga accagcgatt gctgcaatta tttatgcgaa
     5281 cgtagaaact aactttgaat tatttaaaaa cggtgcacaa gcctcaaaag tattaacagt
     5341 tggtctagga aactttgttg gaacgatggg tggtactgga gcgacactag ttgttccttt
     5401 tctgttcatg ttgttcgcta aatcaaaaca attgaaagca gttggtaaag cttcatttat
     5461 tccagtttcg tttgcggtaa atgaaccgtt actatttgct actcctatta ttttgaatcc
     5521 gtactttcta attccatttt tattagcacc aatcgctaac gtatggatat tcaaactttt
     5581 tgtcgatgtc ttggggatga atagctttat atccgtattg ccttgggcga cgcccgcacc
     5641 gattggcttg gttttaggaa ccgggtttag tgtgttggcg attgtattag cggtagtatt
     5701 gattgttgtc gatgcgatta tttatttacc ttttgttaag gcctatgatt ctgaattagt
     5761 agaacaagag ttaaaaaaca ttgctgcaga agaagtaaaa gaagaattgg tcgaagaaat
     5821 ccaagaagtt ggtgcaactg cggcagttat agttgatgaa gcaaaagcat cggttggtga
     5881 aggaaagcgc gttttagtac tttgtgctgg tgccggaacg agtgcaatgt tggctaatgc
     5941 attagaagag ggcgcaaaag agacaggtgt taatctttct gctaaagcag gagcttatgg
     6001 ttctcattat gatatgatga aaaattttga tttaatcgtt ctagcacctc aagtgaattc
     6061 ctatttggat gatctccgta aagatgcaga caaattaggg ataaaagtaa ttgcaactaa
     6121 aggtgcagaa tatatcaatt taacacgtga tcctaaagga gcagttgatt ttgtattgaa
     6181 gcaattttag agcttgctta gattaataaa gcgctagcca acttagctag cgttttatct
     6241 taaacacaat ggtaagcgct atcttttttt gctgtttaaa tttcgggtat tctttttgag
     6301 gcgtcgattg taaaattaag ctagacaact aaaaagatca tttgtgctac actcaaaata
     6361 gttccgacca aagaattaat aaggagtgag tacaaatgac ctacacccat cttacaacag
     6421 acgaactagt aatcatagag tcttatttca atatcgagaa acctgtcgct cttgtcgcac
     6481 aaagtttaaa tcgctctaga caaaccattt ataatgttta ttc
//
LOCUS       DADOZM010000142         6450 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.146, whole genome shotgun sequence.
ACCESSION   DADOZM010000142 DADOZM010000000
VERSION     DADOZM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.146"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            122..1129
                     /locus_tag="J8I47_002447"
     CDS             122..1129
                     /locus_tag="J8I47_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ6323864.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1141..2145
                     /gene="neuB"
                     /locus_tag="J8I47_002448"
     CDS             1141..2145
                     /gene="neuB"
                     /locus_tag="J8I47_002448"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAZ6323865.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2142..3290
                     /gene="neuC"
                     /locus_tag="J8I47_002449"
     CDS             2142..3290
                     /gene="neuC"
                     /locus_tag="J8I47_002449"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAZ6323866.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3263..3940
                     /locus_tag="J8I47_002450"
     CDS             3263..3940
                     /locus_tag="J8I47_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAZ6323867.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3930..5072
                     /locus_tag="J8I47_002451"
     CDS             3930..5072
                     /locus_tag="J8I47_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAZ6323868.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5085..6062
                     /locus_tag="J8I47_002452"
     CDS             5085..6062
                     /locus_tag="J8I47_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ6323869.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6062..>6450
                     /locus_tag="J8I47_002453"
     CDS             6062..>6450
                     /locus_tag="J8I47_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAZ6323870.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQ"
BASE COUNT         2405 a          808 c         1230 g         2007 t
ORIGIN      
        1 aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat
       61 atatttttta aagattaaga atcgaattat agatgactat aattcatagg aggagatata
      121 agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat
      181 tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga
      241 aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg
      301 tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca
      361 tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac
      421 aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt
      481 tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt
      541 attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt
      601 ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag
      661 taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat
      721 gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg
      781 agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat
      841 aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat
      901 caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata
      961 tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa
     1021 agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg
     1081 acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg
     1141 atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat
     1201 ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa
     1261 acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat
     1321 aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct
     1381 gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct
     1441 tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc
     1501 tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt
     1561 ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata
     1621 gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt
     1681 aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat
     1741 cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag
     1801 aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct
     1861 caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt
     1921 gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg
     1981 gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt
     2041 cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga
     2101 ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag
     2161 taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag
     2221 ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta
     2281 ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt
     2341 tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa
     2401 atatatttga acaaactagc cctgattttg tattattact aggggataga ggagaaatgt
     2461 tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg
     2521 aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc
     2581 attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga
     2641 gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga ttacctagtt
     2701 ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac gttttgcaag
     2761 tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta gctttgatca
     2821 attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt gatgctggta
     2881 gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt tatattacga
     2941 atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg atcggaaatt
     3001 cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat ttaggaacca
     3061 gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat gaaaatgaaa
     3121 ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg aaaaatgtta
     3181 agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg aatcaaatat
     3241 tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa aatatactag
     3301 tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg aaaccttaag
     3361 attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa tgcaagatct
     3421 gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa aaaaaatcta
     3481 taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa aaaaattgaa
     3541 tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg caaaagaaat
     3601 ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag atgctgtaat
     3661 tggagagcat gctataataa acacaggtag cattgttgag catgattgta gaattaataa
     3721 ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag gtgtaggaac
     3781 tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt ggtgtatgat
     3841 aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt atggcaatcc
     3901 agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta tcaagtcctc
     3961 atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat tggatagcgc
     4021 cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact ggttctaagg
     4081 gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta agactgctta
     4141 atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct acagtaaatc
     4201 ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat acatggaata
     4261 tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac caattaccta
     4321 aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga attttagcta
     4381 ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta ggttctactt
     4441 ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt aacggtaata
     4501 agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa tatgttgagc
     4561 attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa catagcgaaa
     4621 taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga caaatggaag
     4681 tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag gaagcttttt
     4741 ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca aatcgttggt
     4801 tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac gtaataaata
     4861 aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat ttgcaaccag
     4921 tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca gaaaagttat
     4981 ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa caagaatttg
     5041 ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac ataggaatta
     5101 ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac tttaatgata
     5161 ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa aaggaatttg
     5221 gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata aattcagttt
     5281 ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta aatttagatt
     5341 gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata atcgaagtag
     5401 ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga aaagaaattc
     5461 aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac catgtaggac
     5521 agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt tcaaacaaaa
     5581 aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata gatacatttg
     5641 ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat attgattatt
     5701 cggacagttt cactcttatt attgagcatg aaactggtat agttggaact ttaagtgtag
     5761 atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag attttaatag
     5821 aatgggaagg taacccggat tctctatttt catggaatga agaaacttct agtatgacag
     5881 atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact attattgaag
     5941 atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa atggaacccc
     6001 aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt gaggtaaatt
     6061 aatgatgaaa gtaatttttt ttgggttagg gtcaattgga aagagacatc taaaaaatct
     6121 aataagctat ggaaaaaaga attctattaa ttttgatata acagcttata gtagcagtgg
     6181 aaaaaaagag tttgaagaaa taacgtatat tcaaaataga gacgatttgg atacacatta
     6241 tgatatagct tttattacaa atccgacttt taagcatctg gaaacactaa atgtaattgt
     6301 tgaaaaagtt gattacatat ttttagaaaa acctgctttt tctgagagta ttgatcttac
     6361 tcaatataat tatgattaca aaaatattta tgttgctgct ccactgaggt ataaaaatat
     6421 catggaagtt gttcgtaata gtattcaaag
//
LOCUS       DADOZM010000143         6287 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.147, whole genome shotgun sequence.
ACCESSION   DADOZM010000143 DADOZM010000000
VERSION     DADOZM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.147"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..177
                     /locus_tag="J8I47_002454"
     CDS             <1..177
                     /locus_tag="J8I47_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323871.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="J8I47_002455"
     CDS             190..690
                     /locus_tag="J8I47_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ6323872.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="J8I47_002456"
     CDS             787..2634
                     /locus_tag="J8I47_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAZ6323873.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="J8I47_002457"
     CDS             2637..3533
                     /locus_tag="J8I47_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6323874.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="J8I47_002458"
     CDS             3583..3972
                     /locus_tag="J8I47_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6323875.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="J8I47_002459"
     CDS             3959..>6287
                     /locus_tag="J8I47_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6323876.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DADOZM010000144         6207 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.148, whole genome shotgun sequence.
ACCESSION   DADOZM010000144 DADOZM010000000
VERSION     DADOZM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.148"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            96..1046
                     /locus_tag="J8I47_002460"
     CDS             96..1046
                     /locus_tag="J8I47_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAZ6323877.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1067..1600
                     /locus_tag="J8I47_002461"
     CDS             1067..1600
                     /locus_tag="J8I47_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAZ6323878.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            2236..2769
                     /locus_tag="J8I47_002462"
     CDS             2236..2769
                     /locus_tag="J8I47_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323879.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2781..2984
                     /locus_tag="J8I47_002463"
     CDS             2781..2984
                     /locus_tag="J8I47_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323880.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3306..3686
                     /locus_tag="J8I47_002464"
     CDS             3306..3686
                     /locus_tag="J8I47_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAZ6323881.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3668..4582
                     /locus_tag="J8I47_002465"
     CDS             3668..4582
                     /locus_tag="J8I47_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAZ6323882.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            4827..5516
                     /locus_tag="J8I47_002466"
     CDS             4827..5516
                     /locus_tag="J8I47_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323883.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(5637..5723)
                     /locus_tag="J8I47_002467"
     CDS             complement(5637..5723)
                     /locus_tag="J8I47_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAZ6323884.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
BASE COUNT         2299 a         1084 c         1166 g         1658 t
ORIGIN      
        1 tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt tgtttataac
       61 gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc gtgaaatacc
      121 atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg aatctatttt
      181 tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt acttttgacc
      241 aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt aatgatttag
      301 aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct aatgggctcg
      361 atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct aaagatccat
      421 atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta gatgagtggg
      481 ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag actatgtttc
      541 gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag gattttcatg
      601 aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat aatcgtgtta
      661 taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc aaaaagaaat
      721 acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa ccagaaatca
      781 aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat aaagcattgg
      841 ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac gaaaaaatca
      901 aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa aaagagaaag
      961 aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa agtttattat
     1021 ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc aaccacaaga
     1081 aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag caatatcgaa
     1141 acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt ataaggttgt
     1201 attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt tgctagatag
     1261 gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca aaaacgtggt
     1321 tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag aacaacttgc
     1381 cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac aacaactcac
     1441 cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga cttatacaga
     1501 agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa tagacaaaca
     1561 aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa cattcatgac
     1621 aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca gtccgatgtt
     1681 attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc caaattatta
     1741 caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa aaagactgct
     1801 ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat
     1861 gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta aaaaatgact
     1921 ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct taaaaagagc
     1981 ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg ctacaggcgc
     2041 tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt tcctaatgca
     2101 ttctaactat accacattat caaaaatata aattatcttt actaaataaa atattgacaa
     2161 gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata tttttctgta
     2221 tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac tattggggca
     2281 gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt ggtatcatta
     2341 aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg tatggtcgta
     2401 actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga tatcatgaca
     2461 agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt aaccactgga
     2521 gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag cacttctatg
     2581 tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg gactaaaaaa
     2641 ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa tggctggatt
     2701 ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac tagatattca
     2761 acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca ttaaaaacat
     2821 ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct atagctgttg
     2881 ctataattgt taatatgcta agcggagggc aattatacaa atctggttta tttttagtgt
     2941 taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat acattttaga
     3001 agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa ataagcgaaa
     3061 ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc gtggattttg
     3121 agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca ccaaatacgg
     3181 aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt tactcattgt
     3241 acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact gaaagggtgg
     3301 gacttatgag cgaacaacat caaaatttgg ctagcgatca ttctaaaaaa tatacctatc
     3361 gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca aagctgaagc
     3421 agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg
     3481 cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg cgcgatttag
     3541 ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac caacacgacg
     3601 ttgctcctga tgatgctcaa cgtttatccg aaaacctaaa aatcatgcaa aaggagctac
     3661 aaaagatatg gcaacagtta aagtaagtcg aaccacttca tgtagccgag caatcaatta
     3721 cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt atgccaaaag
     3781 tgaaatgaaa caaatacgca cgctttatgg aaaagacgat cgtgtacaag cacacttatt
     3841 gattcagtcc tttcgaccag gagaaattac cgctgaaaaa gccaatcaac taggggcaga
     3901 gtatgcgaaa aaaatagccc ctaaccacca aatcgcgatt tacacacata cagacaaaga
     3961 ccatattcat aatcacattg tcattaactc cgttaatctt gaaactggga aaaaatttca
     4021 agcccatggg caagcatttt taaacaaatg ttatgaccta aatgacgaga tttgtcttga
     4081 acatgactta acaatcacgg aacgaggaaa gaaaaccgaa aaacgaacca tgtctgaaat
     4141 taaattaaaa gaaaaaaatg aaccggtttg gaaagacgaa attcggtttg cgattgatca
     4201 aacaatgaaa aacaaaaaaa ctcgaacgta tacccaattt tgtgattcac ttaaaacatt
     4261 cggcattcat tgcttgaatc gcggaaaaaa ttttacttac gaattggtcc acaaaaagaa
     4321 aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt tacatgagct
     4381 ggacagacga gaacgaacct acaatcaaca acgaaccaac gagctacgaa gagctcgagc
     4441 tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactttta cgcctgaaca
     4501 atcggaaatt agaccgactc gaagaaacca tgaacaacac gttgaacgag gaacagacca
     4561 ccaacaagaa ttatcgctct aaaacgttaa aagacatcga aaacgaaata aagagacaac
     4621 gtgcctttct tgcgccttat acccaagaaa ggatacaaac tacgatggat tcacaagtca
     4681 aaaaactcca ccaagcgcta ttagaccagc gaaaccaaga acaaaaaaag gctttaattc
     4741 aacgatcgat ggatattctt gcccccttat gttgcttagt actaacatta ttagcctttt
     4801 ggttggtatg gggagaataa acagacatgg cattctattt accctatcta ctcatttctg
     4861 ccagtatttt aggaattatt tggcttagct ataaggctta tcaaacaaga tattcattaa
     4921 aaggctctaa aattcgattt aagcgatttt tattggtcgg ggttatattt gccttatcaa
     4981 tcgttttaag tggtttattc ggcgttttag agggtaataa acgtgtttca agaagcattt
     5041 tattagggga tatcactcaa aagtatgaag cagcatgtga taaaaagaaa aaagagcaag
     5101 cattagcaca aaaaatggag gaatttaccg cttgttatga ggacatgaac aatatttttg
     5161 tggatcaaga gaaacgttta acagataaaa acatgaaaaa actcactcgt ttttatcaaa
     5221 acttaccaga aaaacagcaa aaagaggtac aagaaaaata tgagcaagta aaaaaagacg
     5281 tgcaatattt taaagatacg cagacagaag aagcttgttg taacttattt agcgatacaa
     5341 tccctttttc aaccagtgaa caagaacgaa aagaacggca acaatcggtc acatacgaac
     5401 gttatgaaac actgcttcag caagcaacaa acatacaaaa tccaacgaaa aaagaaacgg
     5461 cattaaatta tctagaaagt gtcaaggaat ggttggatca gcaacaacaa aattgaattt
     5521 gattagcaat catgttaaac tgaacctgca aatgatcaaa cattagatac acgaaagccc
     5581 cagagaggtc ggattctctg gggctttttt tcaggttgat gtcgccttta ttgattttac
     5641 ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaacttc tacaagaagg
     5701 gggcaaatga accgttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata
     5761 ggcgacaagt tattatacct tacaaaataa aatctgtgtg gtgtttttaa gtatccctta
     5821 ttttcgggtt gttagggctt gccctgaccg cctgtaaggc gctttaagaa caagataagg
     5881 ttttttaccc aagactgtct taaaacgact tagacgagct atcagaggct tcccccacct
     5941 ccgttactga cttgttttta gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga
     6001 gcatagcgat cacacttttt tagcgaccgt agggagcaag gttttgaatt ggcagttttg
     6061 aaaaatgcgc gaacctgttg gtagaaaaaa gaagtggttt ttaggcggga aattttgtca
     6121 ccctctttta taatctcttt ttaaaccttt ttttaaacct ctttaccgtc ggctgtgtcc
     6181 ttactccttc aatggataca gcgtttt
//
LOCUS       DADOZM010000145         6150 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.149, whole genome shotgun sequence.
ACCESSION   DADOZM010000145 DADOZM010000000
VERSION     DADOZM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.149"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            65..2854
                     /locus_tag="J8I47_002468"
     CDS             65..2854
                     /locus_tag="J8I47_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ6323885.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2901..3428
                     /locus_tag="J8I47_002469"
     CDS             2901..3428
                     /locus_tag="J8I47_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAZ6323886.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3449..4639
                     /locus_tag="J8I47_002470"
     CDS             3449..4639
                     /locus_tag="J8I47_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ6323887.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4679..5977
                     /gene="asnS"
                     /locus_tag="J8I47_002471"
     CDS             4679..5977
                     /gene="asnS"
                     /locus_tag="J8I47_002471"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAZ6323888.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2041 a         1142 c         1348 g         1619 t
ORIGIN      
        1 atttttctgc actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga
       61 tattttgaaa ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa
      121 agaagatcgg atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg
      181 tttttcgatc gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg
      241 gatcacgaat caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta
      301 caatcttttg gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt
      361 gaaccatgaa ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt
      421 agaacttgca caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga
      481 aagccttgga tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacagg
      541 tcaacttctt ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca
      601 gattgcgcga ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat
      661 tgaagaaatg aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct
      721 agctttgcag aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata
      781 tccaagaaaa aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga
      841 acagaacaga ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt
      901 gatgatcgaa gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta
      961 tttggctaca cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca
     1021 gatcatagaa aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt
     1081 aattatcaaa agctatcgcc actatattga tttgcagcgt tttaaaggca cacttgtgga
     1141 accgccagaa caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca
     1201 aacgaagact ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc
     1261 agatatcgcg cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga
     1321 ctttgttcgt ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc
     1381 gtttttagta caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt
     1441 gatcgatgaa gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag
     1501 tattaactat ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg
     1561 aatacaaagc tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga
     1621 agaactttct gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt
     1681 gagcggcaag caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt
     1741 ggatcagctg ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga
     1801 gatccagcag ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac
     1861 aaaccagatg cggatcgatt tagcaagttt atatagccta tttgatcaat tagacaagca
     1921 atacttgctg atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt
     1981 tcctgggaac acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt
     2041 gccgtcgact gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa
     2101 gatcggccct aatcgacact atctctctaa gaggcttggc attgaagcag cacctcttaa
     2161 agtgattgcc tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc
     2221 acctgccgtc catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac
     2281 aggtctgatc gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt
     2341 gcagaaggtc tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc
     2401 tcaagggttt ggtggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag
     2461 cgtattgttt ggcgctgaca gcttttggga aggtgtagat ttgcctggag atgctttgca
     2521 gatcgttgta gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag
     2581 gaacgcttat ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc
     2641 agctttgaag ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt
     2701 attgcttatt ttggatcgaa gactagtcac tgcaaaatat ggcgcacgga tcatccatgc
     2761 attaccgaaa aaagtaccga tcaaagagct agctatggag gaaatctatc aggatatcca
     2821 tgaattctta aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa
     2881 atctgctata atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga
     2941 caaaggaaaa aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca
     3001 tcgtgatcag ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag
     3061 aagcagtaga aatcgcaaag aaatatgcaa atcttgagga aaccgatcat ttttattggt
     3121 ttactcgaaa gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg
     3181 ccgttatcat tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga
     3241 ctgaaaacga agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag
     3301 ctgctttagg gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg
     3361 ggaaactgaa ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg
     3421 gaatttaaaa ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga
     3481 accgtctgtc actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga
     3541 tgtgctgagt ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc
     3601 agcaatcgaa gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc
     3661 agagcttcga caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc
     3721 ttcacaaaca gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat
     3781 ccttgacccg caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca
     3841 agtaaaactg gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa
     3901 agttactgtt gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa
     3961 ttctccatcg aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga
     4021 atgggcagta aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta
     4081 taatggcaat gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat
     4141 catcatcaac ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt
     4201 gggaaatcct gtgatcatca atggaatgat cgctgttgct tcccaatcaa ctagcaatcc
     4261 gacagcagtc agtcaatatg cagctgttga agcattgaaa ggtgagcaag atacagtaga
     4321 agaaatgcgg aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact
     4381 gccgggtgtt tctcttgaaa agccgcaagg agctttctat ctttttccta atgtaaaaga
     4441 aacgctacgc atgtgtaagt atgaaaatgt cacacaatgg gtagaagatc ttttagaaga
     4501 aacaggggta gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag
     4561 ttatgccact gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga
     4621 gagtaaaagt caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt
     4681 ggaacaaatt caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc
     4741 ttgggtcgca aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac
     4801 agcctttttc caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc
     4861 taaaaatcta caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg
     4921 ttcaaaattt ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga
     4981 ttatccgatc acaccaaaag aacacggaac agacttcttg atggatcatc gtcatttatg
     5041 gctgcgttct tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac
     5101 gtatgaattc tttaatcaac atcattttgt caaaattgat ccgccgatct tgactggttc
     5161 tgcaccagaa ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt
     5221 atctcaaagt ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc
     5281 attcggtccg actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg
     5341 gatgatcgaa ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca
     5401 atatgtagca ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact
     5461 aggacgagac aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta
     5521 cgatgatgca atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga
     5581 ctttggttca cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct
     5641 aaactatcca aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt
     5701 ggtaatttgt gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga
     5761 acgggcaaca gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa
     5821 agaatattca tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg
     5881 tctaggcctt gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat
     5941 tccattccca cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg
     6001 catcaagtaa ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac
     6061 ttattaccgc agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt
     6121 ttagtttcga gtattaagaa aaattttgtg
//
LOCUS       DADOZM010000146         6135 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.150, whole genome shotgun sequence.
ACCESSION   DADOZM010000146 DADOZM010000000
VERSION     DADOZM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.150"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            102..2543
                     /locus_tag="J8I47_002472"
     CDS             102..2543
                     /locus_tag="J8I47_002472"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAZ6323889.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="J8I47_002473"
     CDS             complement(2873..3577)
                     /locus_tag="J8I47_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323890.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="J8I47_002474"
     CDS             complement(3764..4009)
                     /locus_tag="J8I47_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323891.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="J8I47_002475"
     CDS             complement(4026..4877)
                     /locus_tag="J8I47_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAZ6323892.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="J8I47_002476"
     CDS             5043..6047
                     /locus_tag="J8I47_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAZ6323893.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1964 a         1063 c         1297 g         1811 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaa
//
LOCUS       DADOZM010000147         6063 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.151, whole genome shotgun sequence.
ACCESSION   DADOZM010000147 DADOZM010000000
VERSION     DADOZM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.151"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(451..1233)
                     /locus_tag="J8I47_002477"
     CDS             complement(451..1233)
                     /locus_tag="J8I47_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323894.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="J8I47_002478"
     CDS             complement(1298..2134)
                     /locus_tag="J8I47_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6323895.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="J8I47_002479"
     CDS             complement(2152..3561)
                     /locus_tag="J8I47_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6323896.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="J8I47_002480"
     CDS             complement(3575..5398)
                     /locus_tag="J8I47_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323897.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1886 a         1299 c          972 g         1906 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 ttt
//
LOCUS       DADOZM010000148         6047 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.152, whole genome shotgun sequence.
ACCESSION   DADOZM010000148 DADOZM010000000
VERSION     DADOZM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.152"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            202..274
                     /locus_tag="J8I47_002481"
     tRNA            202..274
                     /locus_tag="J8I47_002481"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Lys,seq:ctt)
     gene            436..657
                     /locus_tag="J8I47_002482"
     CDS             436..657
                     /locus_tag="J8I47_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323898.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(981..1226)
                     /locus_tag="J8I47_002483"
     CDS             complement(981..1226)
                     /locus_tag="J8I47_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323899.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1220..1621)
                     /locus_tag="J8I47_002484"
     CDS             complement(1220..1621)
                     /locus_tag="J8I47_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ6323900.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1793..3601)
                     /gene="uidA"
                     /locus_tag="J8I47_002485"
     CDS             complement(1793..3601)
                     /gene="uidA"
                     /locus_tag="J8I47_002485"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAZ6323901.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3629..5395)
                     /locus_tag="J8I47_002486"
     CDS             complement(3629..5395)
                     /locus_tag="J8I47_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAZ6323902.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(5408..>6047)
                     /locus_tag="J8I47_002487"
     CDS             complement(5408..>6047)
                     /locus_tag="J8I47_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ6323903.1"
                     /translation="SETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKR
                     EIEYSQSEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANK
                     EAIQEEWERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAAN
                     KNKVFFYPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
BASE COUNT         1887 a         1148 c          776 g         2236 t
ORIGIN      
        1 tattgatcta tctttcagca aattataagc tttatttttt ccaatattta acattttagt
       61 taaatcagta attttcaata tcgatggata actttcaaat attttttcat aatcttgaaa
      121 atccataaaa ttcactctca attctgataa taagtattgc acttttttat tttaggtgtt
      181 agaataacaa agtattaatt tgggctgtta gctcagttgg tagagcagat gactcttaat
      241 catcgggtcg cgggttcgag cccctcacag cccattgggt gccaaaccct taaatacagt
      301 gtaaacgttg gtgcttagca tttagaaaac gtatatactg tatttttttg ttttcaaaag
      361 agactgtagt accccttttt ttcactaaaa gcccactgta gtcattgata tttttgataa
      421 gaaaggaaca taattatgcg taatgaaatt gctaatataa caataatccg agaaacttta
      481 caaaaccata ctgcaaatga gatctctaaa catacaggat taaatttgag tactataaaa
      541 aaactaaaaa gtggagaacg ttcaatagaa aaactaaatt tacatgatgc tatttgcctc
      601 actgaatttg gtcttaaaaa taatagaaaa aatgttgaaa tcaacatatg gaaataattc
      661 ttaatcagct gcttttgtca aaagcacata ggaatttcac ctctctccaa gtttacggcg
      721 agccttacgg aagtatcatt atcccaactt caaatcatcg ctttttattg actataaaaa
      781 ttattttaga atgtttatcg ctccttcttt acagtaagaa atcatggcgc aactaaaata
      841 tgctccttga agtgttaacg tactgataga atgtatttaa atttcaatga tcaataggtt
      901 ttctgtttaa aagaatctac taatccttta actattagaa gattctttta aacagaataa
      961 gtggaaaggg gactttttag ttagttaatt ttaaatttta agatcgcttc tatcaattta
     1021 atttctagct gtcttagcat atactcatct acataggtaa taaattgccc cgcctcatta
     1081 aatccatttg tcaaacataa cttagctata taactatgat aatgtttgag tatcatcttg
     1141 attgaaattg gatcttcttc tagagcagat tggataatgg atagaggaac taatggatac
     1201 tgccgttttt tggggttgtc taccataagt cataccccat ttttaattgt tcaactgcct
     1261 tctttcgttg ataccagata ccacttgtac tcatattgag gattttaccg atttcagtat
     1321 cattaaaacc tgcaaaataa taaagtagga taacctttct tcttatttcc gataaatgat
     1381 caatggctgc cgataacttt tgatctgaaa ttaatatttc cattccaaat aagaaataga
     1441 catcttgttc atcaaaatga gtttgggagt acttaccttc atttgaattc gtcaatggtt
     1501 cctcattatc tgttatgtta atgtttcgtt ttcgaataga cttagtttcg tttcgtaata
     1561 cttttttaca gtaggcatca aattgatgtt gtatagattt ctcattgtaa ttttgttgca
     1621 tgataaatat gctccttttt tcccctttcc actaatacaa caatcagaaa gcgccttttt
     1681 ttcgaaaaaa ctagaaaaaa atatccttct ttaaacagaa aatcttttta taaaaaaaag
     1741 gagttggcaa ttaagcagcc actccttttt tatattcctt ataaaaggta aattattttt
     1801 tataactgaa atttggtatt gatgaccatc gtttctttag atagtgagca atcattttag
     1861 gctttctatc tctagtaaag attcctttct tatttccttg cactcttaaa atcccttgac
     1921 ttgtttcaaa atctgcaaaa ttccacactt gttcaccaac aaaattgtca catttatcta
     1981 ttacttcgtg atttgcttcg taataaccca cttgatactc ttctgtaaac attgttggta
     2041 ctgtatcatg aagtccagct actgtatctg ctccatattc tgtaaacatt attggcgtat
     2101 cgggacatct ttccatccat ccatctagct cttttttcaa cagatatttt gcatgtttta
     2161 ggtctccacc tgaaacatac caaccataat agcgatttaa acataaaaca tcgacaatat
     2221 ctcctacttt acaaatatct ggtgttgact ttaagtaagt aacgattgtt acaggtctct
     2281 tctgagggtc aagttcttta gtcaattcaa ctaaaggttc aaaatattct ttagcgcctt
     2341 cagaatccga atctggttca tttgcaatag accacattac gacactcgga tggttcttgt
     2401 cgcgttctat caattcctta atcgccattt tgtgagcatt ttctgtccgt atttctttcc
     2461 aagtatcatg tttttctacg ttgtcatcta ataaagtagc catgaaattc aaatgtaatc
     2521 ctactgcagg tacttcatca ataactacaa taccttctcg atctgctaag cgcataattt
     2581 cttctgaata aggataatgt gaagtcctaa atgaattagc accgatccat ttcattaaat
     2641 taaaatcttt gatatttact gcttcattca aaccacgacc attaatatac gtgtcctcat
     2701 gcttcccaaa tcccttaaag taaaaaggtt tattattaat caaaaacttc ccattcgaaa
     2761 cctgaatact acgaatgcca aattcttctg tgtacgtatc aatcacttta ttttcgttaa
     2821 ttaaactaac tttcatacta tataagtaag catttagagg ctcccaaagt gttgggttct
     2881 taagttgaat cactccttct ttccccttag cttctgctat aagttgagct tcttcgtcat
     2941 agattgctat tagaatttct ccggtccatt ctgtatcgat attaaattct acatttgcat
     3001 ctgaatcaat tatttttgta ttaatagtga catcctttat atagtctttc ggagttgtat
     3061 acaatttaac gggtctatta atgcctgcat aattgaaaaa gtcaaaatta accaaatttt
     3121 ctttttttat gtctccatct tcctgtatcg tttctttcaa aatccctaca ggtaaagtgc
     3181 tatcgtcaat aacattgttt acagccactg taattctgtt acttccatct tttataaact
     3241 gattaatttc tgattcaaat ggcgtaaacc ctcctttgtg ttgattaatc aattttccat
     3301 tcaaaaatac ttttgcttca tgtgttgcag atccaaaacg aagaactaat ctttcttcta
     3361 ataatttttt aggaagaatc acttcttttt cataccacac ccatccaaca tgatttctaa
     3421 tgttctctgt ttcaaataaa tcattataag aagaaggaac tgaaattgga taactctctt
     3481 tttcaaccat tggttctaaa gcaagttctt cttgtaagtc atcatttgta tctaacttaa
     3541 atctccaaag accgcttaaa tcaattactt gcctcgtctc tgtcataatg ggatataaca
     3601 tgtaaatcac tcctatataa tttaaatatt aaataaatgt atcctttaac ccacaataag
     3661 catctattgg acttattccc ttaaattcag aaaggcccat tagtttgtcc actaatattt
     3721 ctaaagtttc ttgttttcca tcataagtat tgatataagt ttttacttga ggcacatcag
     3781 ctaaatgaaa cggacactgt acagatacaa aaattgttgg aatttcatga atataaaatg
     3841 gaatatccgg agtaccttta cttgctggcc aaacaattct ttgaactgta cctgggttaa
     3901 cattagctat attaataatc aagtcatatt tttcagttaa atctttgatt ggtctcttag
     3961 cagcatatat tccagctata tttttaattt tttcttcttg cgataatcct ttcaattctt
     4021 cttcaggtga aatgtatttt tcaacttcaa atccttttgc tgacaaaatc tgtttcaata
     4081 aatcaatggc ctgaggtcta tttccagcaa ctacttcacc aaacccacct tttaccccat
     4141 gtacatctac cagtaaaatc cttttatatt tttctggtac aataggccaa atatcttgtt
     4201 tatctttaac tagtgtaatt gcttgatcag ctacctcttt aaaaattgat ttattttcaa
     4261 ttaaaccaat tttttccata gcttcttgtt tcggtaacaa aattttattc ttttcttttt
     4321 tatgcaaacc tagtttagcc tttaagccta aaattcttct taatgcatca cgtaaacgct
     4381 cttctgaaat aattcctttg taataacctt ccatcatata attaaagtct tcatctggat
     4441 cattaaaaaa taggaataga tcacatccag ccgcaataga agtgggcaat aattctgacc
     4501 gtttcattgc cccagtcatt cctaacatat gactagcatc agtaactact aatccattaa
     4561 acccgagttt tcctctaagt aagtcagtga ttaattcttt tgataaagtt gcaggaagta
     4621 aatcttcatc tttcagatca agattaaaat gttttgaata agctggcata tggatatgac
     4681 ctgccataat tgaaggtaat ccagcttcta ttaagcctga gtatactttt ccaaaagaat
     4741 tatcccattc ttctgtactt aaactattaa tactaaaaga tagatgttga tctcgttcgt
     4801 caataccgtc accagggaaa tgtttagctg caggcaaaat tccactctct tgaatacctt
     4861 tcatataagc taaactaagt tctaaaactt tatcagggct ttgtccaaaa gatcgactag
     4921 aaatgatagg atttctccaa tttctattaa tatctacaat aggagcaaaa ctccagttac
     4981 atccaatagc cgatgcttca attccagaaa ctctccctaa ttcataagca tatttcgcat
     5041 catttgttgc accaattttt acttctacac caacttcggt accatctgta caagctccgt
     5101 taccacctgc ttctgtatta gcagcaatta ataaaggaat tttgctttct gtttgtagga
     5161 tatagttttg atcatatacc tcatcagcag ttcctggatt atatcgtaca gctccaatat
     5221 ggtactgatt gaccatacta cttaagtaat ttttatcacg actagatccc atattaacaa
     5281 ataattgacc tattttttct tctaaagtca tagcatcaat cgtctcttca atccaagaaa
     5341 tagcatcttc atctaaatga tatggttttt ttcgtaagtc tacttttata gtcattttct
     5401 ttctcctcta cttattagta agatttttcc aaaaagtatc tatatatttt tgttcaatat
     5461 ctatatactc tcccgataaa catttaaata aaatatcagt tcttaagttt tcccatgatt
     5521 ctttttcttt attaaccaaa ataggataaa agaaaacttt attcttattt gcagcttcta
     5581 gatcaccagg agaatcacca atcattaata tcttatccaa atcacaacca ttttcaatta
     5641 cacttcgaat aatgtcttct ttttttcctt tatcttgaca acacagttca tcaactaagg
     5701 ataataattc atgtctttcc cattcttctt gaatagcttc cttattagct gagcttacaa
     5761 taattatctt cccatatttt ttcagagctt ttatagtatc tctagcactt aaaaagggtt
     5821 tatctaatcc tttagcttga ctaatctgtc gattgacttc ttccgaccat tttaaagctt
     5881 ttaataaatc ctctgactga gagtattcta tttccctctt caaagatgga ttagacaatt
     5941 cttttgttac agatacccag tgctttaatc gattaatcga tggtacatca tttttttcta
     6001 atgttttaac taaacctaaa aatctgttta ttccacgagt ctctgaa
//
LOCUS       DADOZM010000149         5925 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.154, whole genome shotgun sequence.
ACCESSION   DADOZM010000149 DADOZM010000000
VERSION     DADOZM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.154"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(76..1170)
                     /locus_tag="J8I47_002488"
     CDS             complement(76..1170)
                     /locus_tag="J8I47_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAZ6323904.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="J8I47_002489"
     CDS             1359..2120
                     /locus_tag="J8I47_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ6323905.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="J8I47_002490"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="J8I47_002490"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAZ6323906.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="J8I47_002491"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="J8I47_002491"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAZ6323907.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="J8I47_002492"
     CDS             complement(3760..4773)
                     /locus_tag="J8I47_002492"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAZ6323908.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="J8I47_002493"
     CDS             4938..5858
                     /locus_tag="J8I47_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAZ6323909.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DADOZM010000150         5903 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.155, whole genome shotgun sequence.
ACCESSION   DADOZM010000150 DADOZM010000000
VERSION     DADOZM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.155"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..985)
                     /locus_tag="J8I47_002494"
     CDS             complement(<1..985)
                     /locus_tag="J8I47_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAZ6323910.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="J8I47_002495"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="J8I47_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAZ6323911.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="J8I47_002496"
     CDS             1810..2964
                     /locus_tag="J8I47_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAZ6323912.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="J8I47_002497"
     CDS             3179..4147
                     /locus_tag="J8I47_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAZ6323913.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="J8I47_002498"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="J8I47_002498"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAZ6323914.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="J8I47_002499"
     CDS             5177..5785
                     /locus_tag="J8I47_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAZ6323915.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DADOZM010000151         5790 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.157, whole genome shotgun sequence.
ACCESSION   DADOZM010000151 DADOZM010000000
VERSION     DADOZM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.157"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(99..1229)
                     /locus_tag="J8I47_002500"
     CDS             complement(99..1229)
                     /locus_tag="J8I47_002500"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAZ6323916.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="J8I47_002501"
     CDS             complement(1877..3079)
                     /locus_tag="J8I47_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323917.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="J8I47_002502"
     CDS             complement(3139..3342)
                     /locus_tag="J8I47_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ6323918.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="J8I47_002503"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="J8I47_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="J8I47_002504"
     CDS             4879..5691
                     /locus_tag="J8I47_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323919.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DADOZM010000152         5747 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.158, whole genome shotgun sequence.
ACCESSION   DADOZM010000152 DADOZM010000000
VERSION     DADOZM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.158"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1126)
                     /locus_tag="J8I47_002505"
     CDS             complement(<1..1126)
                     /locus_tag="J8I47_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAZ6323920.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
     gene            complement(1162..1872)
                     /locus_tag="J8I47_002506"
     CDS             complement(1162..1872)
                     /locus_tag="J8I47_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323921.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1940..2755)
                     /locus_tag="J8I47_002507"
     CDS             complement(1940..2755)
                     /locus_tag="J8I47_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAZ6323922.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2766..3734)
                     /locus_tag="J8I47_002508"
     CDS             complement(2766..3734)
                     /locus_tag="J8I47_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6323923.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4497..>5747
                     /locus_tag="J8I47_002509"
     CDS             4497..>5747
                     /locus_tag="J8I47_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAZ6323924.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1771 a         1183 c          915 g         1878 t
ORIGIN      
        1 tttttataac agttgacatg taagcctctt ctaacgtttg attcaagccg agaatttgtg
       61 gatatacctc tgctactact tgtccttcgt tcattttttc tgctgtcttt tgagtaacca
      121 tttccaaatc actaatcgaa ttaaaaacgc cttcaggtaa ccgctttcca aattcgacaa
      181 gaattgacgc agctttataa cccgctttat ctccttccgt tgtcaaacag caaggaatct
      241 ctacttcatt gaatgcagca acttctgctt taattgcctc atagtcaatc tcgggtgaag
      301 ctactcctga ctgataaaca ccgtaaatat ctccacttct atccgcaaca acaccatcta
      361 ccatgatctt cactccacca aagcggacct tttcagggaa aactttttcc ttatactcat
      421 atagaagttc atgctgtaga ggttcttgga ctgcctgcac atataggtta ctaagtattt
      481 tcctctgagg tatattagaa agaaattctg cgtcatcaaa aacgatttct ttcattgata
      541 taacaccttt tgaaagcagt aatgcttcaa attcagcgta aacctctgct aaaagctttt
      601 tgtttgctag catttcctta attaataata ctcgactttc agcactggtt tctttttctg
      661 taaaaccata agtctgctta gcaatagcat tcatccaaca atggctgcga tttttgtcga
      721 tcgctgtgat tggaccaaag tggtttattt cattcaatag ctcttctgat gggtcagttt
      781 tccacagggc agcatcccag ccatgagcat aaattggttc atctgctttt ttatggttca
      841 ttacagcttt aatacgctct acagcctcgt tagctgaaga tgtatcgctt aaatctatgc
      901 cagcattcat agccaaataa ccactgaaaa atacatgatt atcatgaatt gctggcataa
      961 cgaattgatc atcataagca attaattccg tgttttcatt taatagatca ctgtaattcc
     1021 agttgttttc caccttttca atgatccctt cattgattac aatataacct ttgaaaggtg
     1081 gctgctctcc atcaaaaatc atttttgacc gcaatatttg cttcataaat aaaatctact
     1141 ccttgaaatc caaacaggaa atcataagta aaaacgatat acttctccca ggtaatattg
     1201 attacctaca tgaattggta tgttgttttg atcgttgatt aaagtatcca taaagaaaaa
     1261 gggcgtccca acagctactt ccattatttt ggcaatttgg tcatcagctg tgacaatttc
     1321 aagtgcagtt tccccaggat tgatggctaa ggtaccttgg actttaagta cttcatacaa
     1381 agaatcagtc agctcttcct ccattaagaa actatatctt tcataagaaa aataattgtt
     1441 ttctaacata atggggatgt tatccgccat tcgctttctc tggatatata aaacatcatc
     1501 accttgagcg atatcgagtt tttttgccat cccttcagtt gctggaatga tcttgcgttc
     1561 caaaacaatg ctagtcggtg taaaaccatt agatttacaa ctctcactga aacccataat
     1621 atattcaatt ttacgaaaaa ctttatgccg gttgacaaaa gttcctttcc cttgtttttt
     1681 aagtaggtac ccttcttcta ccagttcttc tactgctttt ctcactgtga ttcggctgac
     1741 ctcataactt tctatcagct ctgattcagc gggaattttc gtattctcgg gatactcccc
     1801 tgtattgatt ttatttttta atatttcttt taattgtaag tacaatggta attgtgcatt
     1861 tgtatccagc atttttttaa cctccacttt accgggattt ctttctttag gttatcaaat
     1921 gcaggaattt aaaacaagct caatcgtaat gaataccaaa gccgaacgaa ccgtcaacca
     1981 gacaagactt cccagcaaat tcattcccct ttttcatcgc ggtgactata tcgtcccctt
     2041 cgagaaaata aagtaggaaa gctgttaaga aagaatctcc cgctgccatt gtatcaactg
     2101 gttcttctag cacggggttc acttcataaa actttgctcc atcataacca atagccgttt
     2161 tttctcctcg ggttgcaatc aatacatcat ttcgctcaca aaaatgcttc atcaaaaact
     2221 gctttacttc ttcatcggaa aagtcttcta tagaaaagaa gccaaaatca actttatcaa
     2281 ttgcccgctg aacctcttct tccgtaaagt cgtcagaaaa atcataagaa atctttccag
     2341 aaacattttg taactcttct aatagatgat tgacatggct atataatccc atatgaatca
     2401 attcaaactg gtttaaatat tctctgtctt cttcagtaag ctgaatcggg tattcacgag
     2461 taacacctcc cgaattgctg ccaaggaaaa tccggtcgcc ctcatcaatc ttcactctgg
     2521 cacagccatt ctcgcctttt tcaaagcgag agtgactgtt gtccagtcct atttctttta
     2581 ttgctgataa aacatgagct gcctcctcat ctgagccaaa aactcccata aagctacttt
     2641 catgtcccag tttttttgca aaaactgcaa aattcaatga atttcctcca ggatacataa
     2701 ttcccagatt ttcatattta tcgacgacgt tatcacctaa gcctaatacc ttcattttaa
     2761 tcctcctagt actccacttt ccccatatag cgacgaacat ctaaatcatg attgcgtaca
     2821 tctgctaaag ctgaacgata ttctgaaaga atactgtaga ataaaatggg attgaagaat
     2881 tcagaaactt catctgatat catatccaat cctagttcct ttgcatcaac gatttctact
     2941 ctttctgcat atttatcaag gaatcgtttt gcccgttcat ctaaagcacg agtgcgtcca
     3001 atgctcatca agagaataaa attcgtatct ttgtctgtta cttcaaaagg tccatggaag
     3061 aattctccag aatgaacaga agttgaatca agccattgca tttccatcaa agagcaaatt
     3121 gaaaaaccat aagcatgtcc atatgatggt ccactaccta agatataaaa cattgtttca
     3181 tctttgtact tatcagcaaa cactttaata cgatcctgca cctgcttaat gccattttca
     3241 attacggtat cgataacctt catgccttct ctgaatttac cgtaatgttc aaatgtttcg
     3301 gtaacattga ccaaagatac agttaaatca agaatgatgg ccataggatt attgactatt
     3361 tttgcatcat ctccccattc gtaaaggaaa ctatgatcag aaacctttaa taaatccgca
     3421 gttttattat gagtcagacc aacagtcaca gctcctctag cttgagcgac ttctcctgct
     3481 tttaccgttt ctgctgtatt tcctccatga gaacatagaa tgacaacaga tttatcatct
     3541 aattgctttg gggttacatg gttaaattcg ttcgctgtat ataagcctgt aatacacttc
     3601 gatgcttcgg ctttaagaaa ataatctgag acatacatat ctaccagcga accgccacaa
     3661 gccaccaaat aaattccaga aatctctcca tcattttttt ctacaattgc gttaaccgtt
     3721 tttgctacat ccatcaacat ttcctcctta aaattttaaa aatgttaatt ttatccaaaa
     3781 taaaagtaac tgattaaatg taaagatatc acataacgtc atataacgtc atgtgacata
     3841 attgcattgt atccgctttc tttgtgtttg tcaataggaa aaatttattt ctctaaaaaa
     3901 tataaataat ctgcagtgaa acttgagtac atatactaat ttcgaaaaat gcctatgatt
     3961 taaataatta tggtgctggg atgaaggttt ataagtttgt cttgatttgt ttagaagaaa
     4021 tcgtttttgc acacagttga attaggaaac tactggagtg gaaaataaag tgatttgacg
     4081 ggtagatggg tgcacttaaa tttgaaaatg gcttaaaatg ctttattttg aattgcagaa
     4141 tttcttttag tacgcaattt atggtcttaa aactgattaa aaagtgatac attgatttta
     4201 atacaattta tgatacaaaa aatgacctcc tttcttaaat aaaagaggtc attttgtttt
     4261 gtctcataat cgattgtttt tgatacagta gttatattca gaaaaaataa agactcttct
     4321 gagtttataa tcctttggat tatatacgga tacagatgta gacataaaac aagctcaatt
     4381 ttcaaaaatg gcattactca cctttttggt gaattatttg tataaaaaaa atatagaaaa
     4441 gacaccctga aatcctttag aattaatttg gctaccaatt cataggagga ttcaagatgt
     4501 ctcatactca gcttatcaaa gataccctaa atattctaga cctaaatatc tatttcgagg
     4561 aaaattgttt aactaaagaa aaatacaaag ggcaaatttg cttgatttac aaaggaactc
     4621 ttctgtacaa gccagaagag tgttcccatt gtctttgtgt cgtcccctct aggatcattc
     4681 gttgggggac gacaaccgtt cggttgttac tgaatgatgt gtctgagtat cgcacttatc
     4741 tggagctgaa gaaacaacga tttaaatgta agtcttgcca aagaacattc gtagcggata
     4801 cctctgttgc tgaaaaacac tgtttcatta gtcaaaaagt tcgttggtct gtcattgctc
     4861 gcttaaaaga gaatacttcc atgacggaaa tcgctcgtca gaaaaatatt tccacttctt
     4921 cggtctatcg tgtcatgaag cgattctacc gcccattgaa tcctttcaaa cagactttgc
     4981 caaaggtctt atgctttgat gaattcaaat cggttcgaag tgttgccagt gcaatgagtt
     5041 ttatcatgat ggacggacaa agccatgcat tattggatat tgtcgaaaat cgacgcttgc
     5101 cttatttaga acgctatttt tctcggtttt cattagcgac tcgtgaagcc gttcaattga
     5161 ttgttattga tatgtatgcg ccttatgttt cgttggtgaa aaagcttttc ccgaatgctc
     5221 agttgatcat cgatcgtttc cacatcgtcc aacatattgg cagaactttc ttgaatcatc
     5281 gagtgaaaga aaccacggta cgacttaaag gaaattccca ctctcaacga gggttaggga
     5341 aaaaattgaa gcgctattgg aaactcctac aaaaagaaga agcaaagtta aactatgaaa
     5401 aacgggtttg gcgtgccagt tttaaagatt atttgactga aagtgagatt gccgatcgac
     5461 tgttagctgc ttgtcccaac ttacgccaag gctatcagtt ataccaagac attctttatg
     5521 cggtaaagaa aagagatcag tcactatttg aggattgttt aactagagac attagcggtc
     5581 tgccagaaac ctatacaacc actttacgaa cgtttaaaaa gtttctgcca cagatccaaa
     5641 atgcgcttca ctacagctat tcaaacggac cattggaatg cttaaacaac cacatcaaag
     5701 tattaaaacg caatgcgtac ggctttcgca acttttacaa tttcaaa
//
LOCUS       DADOZM010000153         5589 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.159, whole genome shotgun sequence.
ACCESSION   DADOZM010000153 DADOZM010000000
VERSION     DADOZM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.159"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(166..624)
                     /locus_tag="J8I47_002510"
     CDS             complement(166..624)
                     /locus_tag="J8I47_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ6323925.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(637..1962)
                     /locus_tag="J8I47_002511"
     CDS             complement(637..1962)
                     /locus_tag="J8I47_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAZ6323926.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1979..3391)
                     /locus_tag="J8I47_002512"
     CDS             complement(1979..3391)
                     /locus_tag="J8I47_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6323927.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3408..4172)
                     /locus_tag="J8I47_002513"
     CDS             complement(3408..4172)
                     /locus_tag="J8I47_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAZ6323928.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4276..5151)
                     /locus_tag="J8I47_002514"
     CDS             complement(4276..5151)
                     /locus_tag="J8I47_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6323929.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5406..5490)
                     /locus_tag="J8I47_002515"
     tRNA            complement(5406..5490)
                     /locus_tag="J8I47_002515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5454..5456),aa:Leu,seq:caa)
     gene            complement(5508..5579)
                     /locus_tag="J8I47_002516"
     tRNA            complement(5508..5579)
                     /locus_tag="J8I47_002516"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5545..5547),aa:Gln,seq:ttg)
BASE COUNT         1722 a         1174 c          826 g         1867 t
ORIGIN      
        1 ttaagaatcc tgaaaataag ttttctaaat cataggagca acaaaaaaat tcgataatta
       61 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
      121 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      181 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      241 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      301 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      361 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      421 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      481 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      541 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      601 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      661 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      721 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      781 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      841 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      901 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      961 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
     1021 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1081 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1141 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1201 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1261 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1321 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1381 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1441 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1501 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1561 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1621 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1681 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1741 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1801 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1861 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1921 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1981 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     2041 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2101 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2161 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2221 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2281 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2341 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2401 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2461 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2521 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2581 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2641 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2701 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2761 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2821 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2881 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2941 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     3001 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3061 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3121 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3181 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3241 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3301 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3361 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3421 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3481 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3541 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3601 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3661 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3721 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3781 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3841 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3901 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3961 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     4021 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4081 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4141 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4201 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4261 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4321 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4381 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4441 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4501 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4561 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4621 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4681 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4741 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4801 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4861 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4921 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4981 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     5041 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5101 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5161 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5221 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5281 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5341 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5401 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5461 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5521 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5581 gaataaaaa
//
LOCUS       DADOZM010000154         5520 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.160, whole genome shotgun sequence.
ACCESSION   DADOZM010000154 DADOZM010000000
VERSION     DADOZM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.160"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(72..542)
                     /locus_tag="J8I47_002517"
     CDS             complement(72..542)
                     /locus_tag="J8I47_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAZ6323930.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="J8I47_002518"
     CDS             872..2512
                     /locus_tag="J8I47_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAZ6323931.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="J8I47_002519"
     CDS             2666..3988
                     /locus_tag="J8I47_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAZ6323932.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="J8I47_002520"
     CDS             complement(4056..4490)
                     /locus_tag="J8I47_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323933.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5392)
                     /locus_tag="J8I47_002521"
                     /pseudo
     CDS             complement(4555..5392)
                     /locus_tag="J8I47_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1648 a         1101 c         1007 g         1764 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     4981 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5041 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5101 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5161 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5221 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5281 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5341 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5401 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5461 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
//
LOCUS       DADOZM010000155         5469 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.161, whole genome shotgun sequence.
ACCESSION   DADOZM010000155 DADOZM010000000
VERSION     DADOZM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.161"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..287)
                     /locus_tag="J8I47_002522"
     CDS             complement(<1..287)
                     /locus_tag="J8I47_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6323934.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="J8I47_002523"
     CDS             429..1547
                     /locus_tag="J8I47_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ6323935.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="J8I47_002524"
     CDS             1675..3051
                     /locus_tag="J8I47_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAZ6323936.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="J8I47_002525"
     CDS             3452..5032
                     /locus_tag="J8I47_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAZ6323937.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="J8I47_002526"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="J8I47_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
BASE COUNT         1835 a          897 c         1117 g         1620 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctg
//
LOCUS       DADOZM010000156         5460 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.162, whole genome shotgun sequence.
ACCESSION   DADOZM010000156 DADOZM010000000
VERSION     DADOZM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.162"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            74..1585
                     /gene="gadC"
                     /locus_tag="J8I47_002527"
     CDS             74..1585
                     /gene="gadC"
                     /locus_tag="J8I47_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAZ6323938.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1598..2998
                     /locus_tag="J8I47_002528"
     CDS             1598..2998
                     /locus_tag="J8I47_002528"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAZ6323939.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3163..4603)
                     /locus_tag="J8I47_002529"
                     /pseudo
     CDS             complement(3163..4603)
                     /locus_tag="J8I47_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4996..5460
                     /locus_tag="J8I47_002530"
                     /pseudo
     CDS             4996..5460
                     /locus_tag="J8I47_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273566.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1740 a          884 c         1040 g         1796 t
ORIGIN      
        1 tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt aaataggaaa
       61 gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg ccctgacagc
      121 ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat tacatttggt
      181 tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat gtgctgcaga
      241 aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta gtcaaacttt
      301 aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta cagtaggttt
      361 tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt ttccagagct
      421 taatacagat ccatttatca agtttattgg tgtattagtc attttctggg tactgacttt
      481 ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat ttgtcattgg
      541 aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta tcggcggtaa
      601 tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc agctttcaac
      661 tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt cagcatcaca
      721 tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt tactagtgat
      781 tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta ttccacaaca
      841 tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt tacattttaa
      901 cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg gcgttatggg
      961 tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg ctcagcgagg
     1021 tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc cgttgattat
     1081 gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg gaggaggcaa
     1141 caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg taggatattt
     1201 actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa aacgaactta
     1261 caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt ttattttatc
     1321 agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta gtgagcaccg
     1381 aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac cttttatcat
     1441 ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc acgtgaaagc
     1501 tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg ttacaaaaaa
     1561 tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa aagataatca
     1621 agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg ttgacttgcc
     1681 taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat tagtacaaga
     1741 cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc aaacgtatat
     1801 ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa ttgataaatc
     1861 agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg ctgatttatg
     1921 gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt cagaagcctg
     1981 catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa aattaggtct
     2041 tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag tttgttggga
     2101 aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg aaaaacatat
     2161 gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg gtattgtagg
     2221 tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga atgatttagt
     2281 tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg ctgcatcggg
     2341 tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat tgaaaaatgt
     2401 tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg tgggttgggt
     2461 cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta gttacttagg
     2521 tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac taattgggca
     2581 atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc aaagaactca
     2641 tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg aaatcattaa
     2701 tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca atcgcgaatg
     2761 gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc cagcctaccc
     2821 attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag cagactttgg
     2881 aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag agttgaataa
     2941 agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta ctcactaaaa
     3001 ttcttcagag aaatataaaa gaagttaatt aactaataaa aggagttgtg attaccacac
     3061 agctccttaa atctatttga ataaatatat gatattcatt tgttacactc tcaatcttag
     3121 ctgttgttct attcatcatt actcttattg taagtagttc agtcaaattt gcttgttcaa
     3181 ttgatcgata acagtatgaa ttgtttgaaa agtaatcgga tatataattg ttttctcact
     3241 acctaagcct tcttggtata aagaactatc gcaaagtata atccgctgag tacaaaagaa
     3301 agtgtcgtct actatttctc taagttgttt acaaatgata ttactaatag aaaagtgttc
     3361 tcccaaaatg tcttctaact gttgtcgata gtataaatac ctaaagtctg acggagctac
     3421 tatgaacact tcaattggag atttccgttt atgctcattt aaaagactta aactaatggt
     3481 tagtttacga atagaattct cattccaacg cagaaataaa ttgttttgtt ttcgccattg
     3541 ttcaagaatt tcatgaactt ctaagtacaa agcgcgttgc tcttcattta acaagtaagt
     3601 cttatcaggt tgaaatatct gaatatcagc ccaaagtgag cggataaacg ggagaagtgc
     3661 tttatcaaaa agtatctgat tttcttcaga tatattcaga gataagataa gtttttgact
     3721 gagctcaatc accaaagggt tatctgctac atggttttga taaacaacgt tcaagtcttt
     3781 ttgtaacaat tcaaagttat cgcatagata atttctagag ttgaatagaa agtagatata
     3841 aaggcattca tcttcgtcat gacataaaga gaaagaaagt tgctctaatc ctttctgtat
     3901 tagctgaaac aagggagtat gtcgggcaac tcttttttct tcctgactaa aatatattgg
     3961 gttagtctta gatctctgca aaccgatagc gatacctaat aaaataaatt gtttttcaac
     4021 tgtagggtaa cttcttgaaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct
     4081 ttgaataatt gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt
     4141 tagtaacaaa cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta
     4201 ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga
     4261 aatgaaggta gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag
     4321 tacattgaaa aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga
     4381 agagtcgaat gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg
     4441 gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagac agtgagtggg caaacgttca
     4501 attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc tcacaaatgt
     4561 aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc ctcctagtca
     4621 ttttatacat aactattata actctaccaa tcggaatagt cctgtaatat attgaatcaa
     4681 aaatataaat tataaaataa ttagatgggg tatttggttt aagtatccct tatatagtaa
     4741 taaatttata ggtgtgattc tcgaattaat acccgaaaat gtgagagaat ttttatggat
     4801 tttttaggat acaaattaat tatttttgct tttatcctga ttaccagtcc agtactgtcg
     4861 gtatcccatt ttgcagcaaa atcatgttga taaagtgaca agaagactga tgagatatat
     4921 tatccaaatt gagaagataa ttaatcggat tcttgagtat accctacttt ttaagaaagt
     4981 agggtataca agcgtatgaa taaagacata actttccctg tgatggacgt tcggcttgtt
     5041 ccaattacca aaatacaggc gaacgattat aatcccaatc gagtagcgtc tccggaaatg
     5101 aacttattgg agctttctat cattgaagat ggctttactc aaccattagt ttgttattac
     5161 aaggaaaaag aagagaagta tatcttggtt gatgtgcttc ataggtatcg tgttgctaag
     5221 gaaagactca acttaaaagt tgtaccggta acagttattg ataaacccat ataagaacgt
     5281 atggcttcaa caattcgaca caatcgtgca agagggaccc atgaaataag aggaatggaa
     5341 attgtcatcc aaagattagt aaaaaatgga tggtcagaca agagaatatc caaagaatta
     5401 ggcatggaca ttgaagaaat ttttcgcttt aagcaagtgt ctggtggaat ggcaacagtt
//
LOCUS       DADOZM010000157         5451 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.163, whole genome shotgun sequence.
ACCESSION   DADOZM010000157 DADOZM010000000
VERSION     DADOZM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.163"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(59..2008)
                     /locus_tag="J8I47_002531"
     CDS             complement(59..2008)
                     /locus_tag="J8I47_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAZ6323940.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="J8I47_002532"
     CDS             complement(2050..3108)
                     /locus_tag="J8I47_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAZ6323941.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="J8I47_002533"
     CDS             complement(3227..3682)
                     /locus_tag="J8I47_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323942.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="J8I47_002534"
     CDS             complement(4266..5069)
                     /locus_tag="J8I47_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ6323943.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1589 a         1087 c          757 g         2018 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag a
//
LOCUS       DADOZM010000158         5422 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.164, whole genome shotgun sequence.
ACCESSION   DADOZM010000158 DADOZM010000000
VERSION     DADOZM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.164"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..454)
                     /locus_tag="J8I47_002535"
     CDS             complement(<1..454)
                     /locus_tag="J8I47_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6323944.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYA"
     gene            complement(570..1484)
                     /locus_tag="J8I47_002536"
     CDS             complement(570..1484)
                     /locus_tag="J8I47_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAZ6323945.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1575..2003)
                     /locus_tag="J8I47_002537"
     CDS             complement(1575..2003)
                     /locus_tag="J8I47_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ6323946.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(2008..2382)
                     /locus_tag="J8I47_002538"
     CDS             complement(2008..2382)
                     /locus_tag="J8I47_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6323947.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2668..2853
                     /locus_tag="J8I47_002539"
     CDS             2668..2853
                     /locus_tag="J8I47_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6323948.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2865..3113
                     /locus_tag="J8I47_002540"
     CDS             2865..3113
                     /locus_tag="J8I47_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323949.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3144..3323
                     /locus_tag="J8I47_002541"
     CDS             3144..3323
                     /locus_tag="J8I47_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323950.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3366..3836)
                     /locus_tag="J8I47_002542"
     CDS             complement(3366..3836)
                     /locus_tag="J8I47_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323951.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3923..4621
                     /locus_tag="J8I47_002543"
     CDS             3923..4621
                     /locus_tag="J8I47_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAZ6323952.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4799..5140
                     /locus_tag="J8I47_002544"
     CDS             4799..5140
                     /locus_tag="J8I47_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323953.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5133..>5422
                     /locus_tag="J8I47_002545"
     CDS             5133..>5422
                     /locus_tag="J8I47_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAZ6323954.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1808 a          844 c          969 g         1801 t
ORIGIN      
        1 tagcgtatga aaaaagaaga ttcaatttgg atttaattac actgacgtac ttatttgaca
       61 agtcatcttt atatatcata tcttcaaaaa tattatttaa atctactgta ttaataccag
      121 aaacgatgta agtctcaggt atctttttct ttatagtgtt taagatatta ttcgtaggat
      181 aatatgtaga ctccttaact tgtcttttgt aaataactaa ccattcttcg acaagctcat
      241 gaaatgttag atcaggcttc tgaagtgttt tttcgtttaa ttttatatca atttttttat
      301 ttaactctag caatgccact ttctgagttt ctcgagattt atttttataa gtaatgctca
      361 ctttttttct ttttctagtt tttggatcaa tatatcgctc attatattta tatacttttt
      421 ttccgttttt atcagttttg gtttcaatcc acattgtttt catctcctat ttgttgctat
      481 aataggaatg gataagtaag ccaattatag caggtttatt tttcatcacg tccacaaact
      541 ttggcgaggg agggggcgtg tttttttgtt tagtagctac ctttccatga agtatctgcg
      601 aagaacactt caaatgttgt tccatcattt ttcacttcaa aatatgctgt tccttgtgcg
      661 gatttacccg gttggatggt ttccgaaaag taatctctac tagctaaatc agatttaata
      721 tctgatgaat cataaaattc taaataactt gaacttgcat caaaaggtgt agtccctgtg
      781 ttttctacaa gaaaatctac ctttgcgtaa aataggcctt ctggtttata gaaatcatca
      841 ccgtaatctt ttgatacaga ctggacagtt acgttaatag agttaccatt ttcagaagta
      901 aaaggaactg cttctcctat actatgtgca actgatgccg tctggggttc tgaagacgaa
      961 gttgtagttg tagttgtagt agagcttgaa tctttggatt ttccatcttt atctaagaaa
     1021 gttcgttgat tataatatac aacctgttca gtttttggac taacccaaat taacattgtt
     1081 tcatcatcag aatcagttga ataagtatat tggagcatct ctaatttttt tatatctttt
     1141 gcactatttc catgttcaat taattcagag tgctctttat aataatctcc ttgtgcatcc
     1201 tttgaaatag atacaattaa ttcatcccgt gtgatagcat tgtctaattc tttgtaaaca
     1261 aaatcatttg tccatttacg taacggtgaa ccaagtgtct gataaacttg agttaaatca
     1321 tcaccaactt ttattttatg aacgtctgat gaagttagtt tttttgtttc atattcagat
     1381 gttgatgaaa cagtattttc tttagtgcta gcagaattag gactagtgga gcatccgctt
     1441 aataccaagc ctaaaaaaag caacccaaaa acaatttttt tcatttttaa ttcctcactt
     1501 ctatgatatg atttttttgt aggatcttag aaataaggtt tttagtccgt gttgcagcac
     1561 ggactttttt tacattatat aagaaagcga taagcgcttt ctggaagtcc gtaaagattc
     1621 tttaattcct caattcgttt aggatattga tcattgtctt ctttgtatag agaaacaatg
     1681 agattagcag caaagcaatt agcttcgctt tcagatttgc ttctagatgt tcttgttgat
     1741 acatagtaac tggataagcc acgatgaaaa atagcgtgac ctaattcgtg agcgcaaatg
     1801 tagaatcttt cctcagagtc tctcagttca tcatttaaga agattattgc acgacctcta
     1861 atttcttgaa actgtccttt gggattctcg ataaaaggaa cgtattgaat tttaatgccc
     1921 atcttttcac aaatataaaa aggattagcg gactggtatt tccgcttcaa ctcctcgact
     1981 aaattaatcg tatccatctc cataagctca catctttttg cctttttctt tgtcttcttt
     2041 cacaatatcc cagaaggttc caataaggat atcttttacg cgttggacct gttcaggcgt
     2101 cagtgtttct ccgccataag acatattgac atttgaatct agtaatttat caagttcaac
     2161 tacttcatct tttgttgccc agtcaggaac ttggtttcta ccgagtaaat aatcagtagt
     2221 tacattaaaa taatctgcta caagttgtag tcttttagta ctaggagttc tatttttcca
     2281 ttgataaatc gtgtttttag gtatatttaa ttcttcttcc aattgagtta tacttatgcc
     2341 tcttttatga gctaattcct ttattctatc tagtaaattc attttcgcac ctcacaagct
     2401 acgaaaacac taataaaaaa attagttaaa ggtgttgaca actaataaaa gtgttagtat
     2461 actgttttcg taagctaaaa tattagctaa aaagacttga taactaataa acacttcacg
     2521 gtcggcaaac tctgaaatgt aaattactag gcatttctgt gtcttattta gctatgtcta
     2581 tatactaata aaaatattag ttattgtcaa cgaatattag ctaatttttt agcttacaga
     2641 ttattttttt agaaaggagc tatttttatg tctgagaatt tagacttaaa aattcgagcg
     2701 gagatgagaa aaagaagaat gactttcaaa gaactagctg cgcttatcgg tatttcagga
     2761 gcttatttat cagatattct aaacggcaat cgtgatggaa agaaagcaca acagcatatc
     2821 gaaacagtga aaaaaatatt ggacatccga taggggggta ggcgatgaca aaactaaaaa
     2881 aacaagattt tgtaaaaaaa tacaattatt ctccatctac ttatcaacgt cgaatgtcgg
     2941 aactaaaaaa tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga caagaagttt
     3001 ggatcaatac agaattatac gataaatttt tgtctttcaa atcctataac aggttacgca
     3061 caagaaaggt aacacctaaa gaatttatcg agaagcattt agttgattta taaaaaacag
     3121 aacattttga gaggtgaagg ttaatgggta aattcaacag agcattggta ttcagcgcac
     3181 cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg
     3241 tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa
     3301 agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact
     3361 attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca
     3421 acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg
     3481 agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta
     3541 ggaggaatat ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct
     3601 ttagcaatta atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa
     3661 agttctaaat taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta
     3721 taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt
     3781 ccttttattt ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat
     3841 tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat
     3901 accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
     3961 gactacgagc ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga
     4021 tattgatgca ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga
     4081 accagattca tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt
     4141 gctggctatg gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc
     4201 attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat
     4261 gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga
     4321 tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt
     4381 tatgggagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt
     4441 ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc
     4501 tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat
     4561 ccgtatggaa attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata
     4621 gagctgaagc gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac
     4681 caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt
     4741 gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat
     4801 ggaaaaagaa ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa
     4861 aattgaggaa acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc
     4921 ttgggatcaa gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca
     4981 tctatcgaag cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga
     5041 tgcgctagaa gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa
     5101 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact
     5161 ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt
     5221 atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa
     5281 ctattaatga gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg
     5341 aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg
     5401 ttaaaggaca cacggaaacc ga
//
LOCUS       DADOZM010000159         5289 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.165, whole genome shotgun sequence.
ACCESSION   DADOZM010000159 DADOZM010000000
VERSION     DADOZM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.165"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(19..339)
                     /locus_tag="J8I47_002546"
     CDS             complement(19..339)
                     /locus_tag="J8I47_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAZ6323955.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="J8I47_002547"
     CDS             complement(487..2721)
                     /locus_tag="J8I47_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAZ6323956.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="J8I47_002548"
     CDS             3200..3466
                     /locus_tag="J8I47_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAZ6323957.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="J8I47_002549"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="J8I47_002549"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAZ6323958.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1595 a          984 c         1066 g         1644 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaa
//
LOCUS       DADOZM010000160         5183 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.166, whole genome shotgun sequence.
ACCESSION   DADOZM010000160 DADOZM010000000
VERSION     DADOZM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.166"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            174..518
                     /locus_tag="J8I47_002550"
     CDS             174..518
                     /locus_tag="J8I47_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAZ6323959.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="J8I47_002551"
     CDS             519..938
                     /locus_tag="J8I47_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAZ6323960.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="J8I47_002552"
     CDS             complement(1030..1380)
                     /locus_tag="J8I47_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323961.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="J8I47_002553"
     CDS             complement(1346..2566)
                     /locus_tag="J8I47_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAZ6323962.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="J8I47_002554"
     CDS             2932..3498
                     /locus_tag="J8I47_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ6323963.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="J8I47_002555"
     tRNA            3643..3730
                     /locus_tag="J8I47_002555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..4630
                     /locus_tag="J8I47_002556"
                     /pseudo
     CDS             4064..4630
                     /locus_tag="J8I47_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4743..>5183
                     /locus_tag="J8I47_002557"
     CDS             4743..>5183
                     /locus_tag="J8I47_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6323964.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNA"
BASE COUNT         1896 a          876 c          981 g         1430 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaacgagag tataaaatat tttgtgtaaa tgaaaaaatc catacaaaaa
     4681 aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa gaggacttcc
     4741 ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc gatttaaatg
     4801 aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact gaattaacgg
     4861 cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg
     4921 gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa atacctagag
     4981 atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca aacgatacat
     5041 tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa attgctgatt
     5101 tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac atgactaaag
     5161 ttctgactga agaagtaaat gcc
//
LOCUS       DADOZM010000161         5153 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.167, whole genome shotgun sequence.
ACCESSION   DADOZM010000161 DADOZM010000000
VERSION     DADOZM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.167"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(136..1284)
                     /gene="nagA"
                     /locus_tag="J8I47_002558"
     CDS             complement(136..1284)
                     /gene="nagA"
                     /locus_tag="J8I47_002558"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAZ6323965.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1360..2196)
                     /locus_tag="J8I47_002559"
     CDS             complement(1360..2196)
                     /locus_tag="J8I47_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6323966.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2380..3597)
                     /locus_tag="J8I47_002560"
     CDS             complement(2380..3597)
                     /locus_tag="J8I47_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ6323967.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3980..4063
                     /locus_tag="J8I47_002561"
     tRNA            3980..4063
                     /locus_tag="J8I47_002561"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4014..4016,aa:Leu,seq:gag)
     gene            complement(4303..>5153)
                     /locus_tag="J8I47_002562"
     CDS             complement(4303..>5153)
                     /locus_tag="J8I47_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323968.1"
                     /translation="LTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
BASE COUNT         1599 a         1047 c          820 g         1687 t
ORIGIN      
        1 tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac ttatgtaaag
       61 tgatttttac tagaacacaa aaaaacctta aaaccaaact taagaagttt agttttaagg
      121 ttaacttaaa ttatattatg cgtggaaacg ttccacgcca tctagataag ttgcttccaa
      181 tgtcatatca gggttcaata cgataaagtc tgcatcgcgt ccttgagcga tttttccaca
      241 tgtatcatcg attttacagc tgattgctgg aatcagacta gccatcatga ctgcttgttc
      301 tggtgttgct aatccccagt ctacaacatt tttaattgct tctttcaatt tcaatatact
      361 tccagctaag ttgccagatt ctagacgtgc tgttccttct tttactacta ctggaaattc
      421 gcctaaattg taatttccat caggcatccc accggccatc atacagtctg taattaatgc
      481 gacatgatca tgtcctgctt tttccattag gattcccgca gcatttggat gtacatgatg
      541 gccatcacaa atcaattcag aaaagacatg ttctaaagat aataacgcac ccaccatacc
      601 tggttcgcga tgattcaatc cacgcatacc attgaaagca tggacaaata cacttgcgcc
      661 tgcttcaaca gctttttgcg cttcttctag tgtagcatta ctgtgtccta gagaaacaac
      721 tactccttct tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa
      781 agcaattttt ttgattaaac cacctgaagc ttcttgccat tcatggaaag tatccaaatc
      841 aggatccccg aaataacttg ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc
      901 ttcaaaatag attcctttga ttttagctcc tggtgcttcc tgatacattt tgccgattgt
      961 ctcagctaca tcttttaaac gttctttgct tgacgtcaat gttgttggta aaaatgaagt
     1021 cactccacaa gataacagac cttcagacat cactttgatg ccttctgcat cattatccat
     1081 tacatcatga ttcatatagc cgtgaatatg tgtatctacc aaaccaggag caatccattt
     1141 gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt attcaccaaa
     1201 aagtccatcg gtaatttcaa gatagcctgc atgtttgaca cctgacgcta aaaagaattt
     1261 gtcagcatag ataaaagttc tcattctatt ctctcccttt gtttttctac ctctaaagta
     1321 ctcttattta gagaagaagt caagaatggt ctacaccatt tacaaatttt ttgaaatttg
     1381 tttcacagct tgtgctaagc cttcatagaa tgctgctctt tctggttctt ctttacgctt
     1441 gtatattaag cttttaagca caaataaatc attgaggtaa tagtagctat tcttctcttg
     1501 agcccaatag attccttgat tcacctgttc taacgcttcg tcgatttcat tttgatggaa
     1561 taagaaattt ccataattat aaaaaatttg cgaaaattca ctttttgatc gttcatcagc
     1621 tgattgatgc agcagttgga tgcttcgact caaatagtag cgtgcttcag ccgtttttcc
     1681 tgccacacca tatatttttc cgatacaact aatcagttgt atttctgtgt cagaaacatg
     1741 gattctttct ttttggctag agtagcttaa tgcttctttc aaataataaa gggactgaga
     1801 gatattttga cgcaacgtga attcgcaaac accgagataa taataatagc gttgaaagtc
     1861 agttgcttga tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg
     1921 ataatcacgt ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt
     1981 agtttttcga gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg
     2041 catcatcacc aatacattcg gcagttcctc attattttcg atccgactta atacactttg
     2101 tgaacaaata tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt
     2161 aatgatcgtt ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt
     2221 attcgcattc tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa
     2281 ctaaataata tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt
     2341 tgtctgtggc cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc
     2401 taggaattct ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac
     2461 gattcgaaaa tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg
     2521 atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt
     2581 atcaattttt gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat
     2641 atcattgatt gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag
     2701 cagctcatcg acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac
     2761 attggaacac aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg
     2821 tgacttatct ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt
     2881 ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg
     2941 ttctagacca tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt
     3001 ttgtcgagct acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg
     3061 attattagga ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc
     3121 atcaatatca ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc
     3181 agctaatgca atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc
     3241 tccattgtcc agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt
     3301 gtagatatca ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc
     3361 ttttctagca gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat
     3421 gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt
     3481 gttcaatttt aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg
     3541 accgcgaaca tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac
     3601 ttagccaatc cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt
     3661 caatatcata ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa
     3721 aaaaacacaa acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg
     3781 cccaatccat tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga
     3841 atgcaagtcc aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt
     3901 tctccatctt aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc
     3961 attagtgtgt tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc
     4021 atgtgagcgt tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt
     4081 aattttgctt cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac
     4141 tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg
     4201 tcggaaacaa gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt
     4261 aaaatccctc acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt
     4321 tattataaag gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg
     4381 tacactcatg cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac
     4441 ataaagtata tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat
     4501 taaccttgaa caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt
     4561 gtcaaaactt aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt
     4621 agctatccaa gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt
     4681 attcaggtta ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct
     4741 ttgacccatt gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa
     4801 tatacgcaca taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag
     4861 caataaaaat aagatattca tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa
     4921 atctaactta ttaagaaagt tgaaattttt atattcagga aaccatttat taatttcatg
     4981 tgcagcacgt aatgcattcc ctggagttgt caaagagaaa attaattctg ctaagcttat
     5041 tattgaaaat actgataact tataattata tcgtccattg acaatcaaat ataaactaac
     5101 tatacttgtt agaacaaaaa aacaggcgtt gacttgttct tgattagtta aaa
//
LOCUS       DADOZM010000162         5152 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.168, whole genome shotgun sequence.
ACCESSION   DADOZM010000162 DADOZM010000000
VERSION     DADOZM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.168"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(214..1197)
                     /locus_tag="J8I47_002563"
     CDS             complement(214..1197)
                     /locus_tag="J8I47_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAZ6323969.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            1575..2966
                     /locus_tag="J8I47_002564"
     CDS             1575..2966
                     /locus_tag="J8I47_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAZ6323970.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2982..4022
                     /locus_tag="J8I47_002565"
     CDS             2982..4022
                     /locus_tag="J8I47_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ6323971.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            4041..5126
                     /locus_tag="J8I47_002566"
     CDS             4041..5126
                     /locus_tag="J8I47_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAZ6323972.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         1761 a          802 c          910 g         1679 t
ORIGIN      
        1 aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc
       61 accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct
      121 tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc
      181 tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta
      241 ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag tgatggagat
      301 aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata
      361 tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc
      421 ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg
      481 ttttccttta tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc
      541 cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt
      601 ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata
      661 attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt
      721 tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata
      781 gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca
      841 atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat
      901 tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata
      961 ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc
     1021 aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc
     1081 ttttttataa ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat
     1141 ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag
     1201 atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg
     1261 attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa gatggattct
     1321 ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt
     1381 ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct
     1441 tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt
     1501 gcttataaac taggttttta aaggtgacta tggtaagata atagagatat tattttgtga
     1561 ttaggagtgt atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta
     1621 tattaatgga tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc
     1681 gtggagagat cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag
     1741 tgatttttat tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca
     1801 gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag
     1861 tgacttttgc gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc
     1921 tcggtacgct tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa
     1981 gtcgagataa aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa
     2041 attttggttc aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt
     2101 atgcaaacat caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg
     2161 aagaaactga aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt
     2221 tgaatacaac atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg
     2281 aaagtattat tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt
     2341 tgtttgatat catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca
     2401 cagcaatttt agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca
     2461 caaaaaatca aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag
     2521 caaaatctgg accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat
     2581 ttattcgagc tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata
     2641 tgtcgatcgt aggaccacgt cctgaacgtt ctttcttcgt cgatcagttc aatgcagaaa
     2701 acccttatta ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct
     2761 atggaaaata tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa
     2821 aatattcatt gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata
     2881 aaatgtcctc tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt
     2941 ttccgaatga aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca
     3001 gcataatcgt accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa
     3061 aagaattgga ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa
     3121 cacaggaaat cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga
     3181 ccaatcaagg gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc
     3241 tcttattctt cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt
     3301 tgctagaaaa gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg
     3361 acgagggaag aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc
     3421 acgaagagtt tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat
     3481 ggaacaaatg ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata
     3541 atagttgtga ggatcgaata tttaacttac attattatga atactgtcat caagtgatca
     3601 tgaacccaaa agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat
     3661 accgacctaa caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag
     3721 acgatattaa taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt
     3781 tttctattta tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga
     3841 tattgagtga tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa
     3901 aaaaaataac aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag
     3961 ttggaagctt cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt
     4021 agttaatgga ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag
     4081 gtggagcaga gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat
     4141 ttgatttttt gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa
     4201 gattaggagg ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa
     4261 tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt
     4321 ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc
     4381 atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat
     4441 ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg
     4501 gtgaaagtct gtttcaaaac catccctttg agatagttca taatggtatt gatttagagc
     4561 tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga
     4621 gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt
     4681 tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg
     4741 aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag
     4801 taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg
     4861 taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat
     4921 taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt
     4981 ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg
     5041 ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg
     5101 agagtatata tagtagagat cattaaaaat gttcaagata ttttaggagt ga
//
LOCUS       DADOZM010000163         5142 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.169, whole genome shotgun sequence.
ACCESSION   DADOZM010000163 DADOZM010000000
VERSION     DADOZM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.169"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            49..1023
                     /locus_tag="J8I47_002567"
     CDS             49..1023
                     /locus_tag="J8I47_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ6323973.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1005..1466
                     /locus_tag="J8I47_002568"
     CDS             1005..1466
                     /locus_tag="J8I47_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ6323974.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1516..1980
                     /locus_tag="J8I47_002569"
     CDS             1516..1980
                     /locus_tag="J8I47_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ6323975.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1994..2743
                     /locus_tag="J8I47_002570"
     CDS             1994..2743
                     /locus_tag="J8I47_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6323976.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2743..3573
                     /locus_tag="J8I47_002571"
     CDS             2743..3573
                     /locus_tag="J8I47_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ6323977.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3573..4220
                     /locus_tag="J8I47_002572"
     CDS             3573..4220
                     /locus_tag="J8I47_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6323978.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4318..5094)
                     /locus_tag="J8I47_002573"
     CDS             complement(4318..5094)
                     /locus_tag="J8I47_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6323979.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1743 a          703 c          933 g         1763 t
ORIGIN      
        1 gtaaagttat atgactttat aatacttatt agagtgaggg ataaagaaat gagacaacta
       61 cacaaagaac agattgaaac agcaatggca gcgttaaagg aaaagacaat tgatcatgta
      121 tattttacag catgtggggg ttcacaagcc gttttaatgg ctggtcaata tctatttgat
      181 aaagaattaa ctgtaccttg tcatgtttat actgctaacg aatttaacta tgctatacca
      241 aaaggattat ctgaaaattc tttagttatc acttgttccc attctggcac aacaccagaa
      301 actgtagaag caacaaaaaa agcaagagaa tttggagcaa tgactatttg tttatctaat
      361 gaagaaggat ctcctctatg ggaagcagct gaatatcctg ttcactatga ttggggtaaa
      421 gatgttgatg ctagtgattt aaataaagga attctttatg gtttattatt taatatttta
      481 cttgttttag atggctctaa aaaatgggaa acttgcttga atgaatttga taagttaaat
      541 gatcttgtta ttgctgaaaa gagcaaattt gaaataactg ctaaagaatg gggtaaaaac
      601 caaaaacgag ataaaattat ttacacaatt ggtagcggta tcaattacgg agaaatttac
      661 tccacagcag cttgctggtt catggaaatg caatggatta attctaatgc tattcattct
      721 ggggaatttt tccatggtcc ttttgaaatc actgattatg atgtaccatt catcttagtg
      781 aaaagtattg gagatacaag atttttagat caacgggtag aagacttcac taaaaaattc
      841 agtaataaat taatggtttt agaccaagct gattttgact tagaatctat agatagtgta
      901 gcgaaagaat atattgcagc tgtacttagt ggcgttgtta ttcgccaaat ggtagaagcg
      961 attgcttttg aaagaggcca ttccttagat gttagacgct atatgtggca aatgaaatat
     1021 taattaatag gaggttgcaa aagatggcaa atcaattatt tcacattgtg attacaacac
     1081 attctaccct ttgtgagggt tatttaaagg caacaaaatt aattttaatg aatgacgcca
     1141 caggtattag tgctgttcca tttaaagaaa atactagtcc agatgatttt gaggagagta
     1201 tgaaagaaat aattagtcag catcatttgc aacctctaat tattttaacg gatttaattg
     1261 gaggaacacc taataatgtt tctattaaac agttgaacaa caatagaatt caaattattg
     1321 ctgggattaa tttacctctt ttactggagt tattaatggc ccagtcagct ggtaaatcta
     1381 tagaagaaat agattttaag gaattactga ataactctca aaaaagtatg gttcatatta
     1441 attcgctttt aaaagatgtc agttaaggtg gcaatttcta ttctataaat tttaaaaatt
     1501 gaaggaggaa atagaatgat taaattagtg cgtgtagacc acagattact tcatggacaa
     1561 gtaatatttt cttggactaa acagtatgat attaaccata ttattgtggc tgataacaaa
     1621 gttccaggag atccaatgtc tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa
     1681 ttagacattg ttaaaattag tgatgttaaa gggattatgg aaacatcccc aaatgataat
     1741 tacatgattt taattaaagg acctaaggaa gcttttgaat tagttcaaaa tttaccagaa
     1801 attaaagaaa ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag
     1861 gcagtctttt taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg
     1921 aaaatatttg tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa
     1981 ggggggaaaa taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt
     2041 tattgactcc aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact
     2101 tgccggactt gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt
     2161 aatttctatg ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag
     2221 tattattgcc actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat
     2281 agctatccca gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc
     2341 tcaatttgga catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat
     2401 tattcatatt tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt
     2461 aacagtatat tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat
     2521 tacagacgcc ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt
     2581 aagcacaatg ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc
     2641 ctattcaggt ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat
     2701 ggatcaagtt aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa
     2761 aaaaaacgtt taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc
     2821 atgtgttcgt ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca
     2881 attttaaata aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat
     2941 atggcatttt ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct
     3001 atggaagaac aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg
     3061 attaaaacca gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact
     3121 ttaagaatta ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga
     3181 ccgattttag ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt
     3241 aaattaggat atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg
     3301 gatcgagtaa tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct
     3361 agcatggtgc caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta
     3421 caagacttac ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata
     3481 tataaattga ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca
     3541 gggtttgttt taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg
     3601 acggagtttt aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac
     3661 aagaaaattt aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta
     3721 tttttgatga tatagaaaag tacaatgagg gatgtaacag tttagaattg aagggaaaat
     3781 ttactaaata ttggaaatcg caaaagattg attacttgga tatttttcct aatatactaa
     3841 ttgaacagct tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc
     3901 caatggatac tataaatgaa gttgttacca cttgtaaaat agagtcatat tttgattgtt
     3961 taattagcgg aagagatttg ccagaaagta aacctaatcc tactatattc cttaaagctt
     4021 cagaacagct cagaattcca gttgaagaat gtctggtgat agaagattct tataatggta
     4081 taaaagctgg taaaagagca aatatgaagg ttcttgcaat taaagataaa cgtttttctc
     4141 aagatgtaag tctagcagat gatgttattt acgatattaa atatctgcga caagaagtac
     4201 aggtgaaatt tggaatataa ataagttttt tgatgtattt agagaatatt agtaaaaata
     4261 aaaatcatgc ccaaatctaa tcaaaattga ttgctttggg tatgatttct tttaatatca
     4321 atctattact ttaggatctg aatcattttt atgcaccgta tctaaagtga tattttctgt
     4381 gacttcaata acataactaa ttctatcaca acgaactgtg gattgagaat aatgaaatgg
     4441 ttgattattt ttatctttgc caaccttttc taaaataaat agcggatctc ctactataca
     4501 ttgcaataag tcggaaagct caggtgttgc agataaaccg tttatttcca taatagagct
     4561 cgtagctttg acatcatagt catcttctag aagtttatat aatgaagtat attggctacc
     4621 tttttcaaat aaagttggaa atttttcttc tgatgcgaaa aaattatcaa ttcccatcgg
     4681 ggcaccgtct tcccacatta aacgtttaat attgtaaact ctatcagtta cattgatttg
     4741 taatttatta gctaaaaact catcagcctt tataatttta gaatgtaaaa cagtatatct
     4801 aattttatgt cctcgggtag aaagagaatc gctaaatcca tgaactccag ctaattttaa
     4861 actttctttt ttttgagtta caaaggtccc tttccccctt aatcgttcaa ctgttccact
     4921 ttctgctaaa agtttaaaag cttttcgaat ggtaatctta cttactccat aaattttaca
     4981 taattcttct tctgtaggta attgctctcc tggaagatat tttttttcac gaatatcgtt
     5041 acgtatagaa gcttctactt gtaaatataa tggtctatta tcttgaaaat tcatagtaca
     5101 ctccctaaaa agttatactt cattatatca caatttatta aa
//
LOCUS       DADOZM010000164         5141 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.170, whole genome shotgun sequence.
ACCESSION   DADOZM010000164 DADOZM010000000
VERSION     DADOZM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.170"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(4..1029)
                     /locus_tag="J8I47_002574"
     CDS             complement(4..1029)
                     /locus_tag="J8I47_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323980.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="J8I47_002575"
     CDS             complement(1274..2545)
                     /locus_tag="J8I47_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAZ6323981.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="J8I47_002576"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="J8I47_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="J8I47_002577"
     CDS             3788..4261
                     /locus_tag="J8I47_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6323982.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="J8I47_002578"
     CDS             complement(4434..4988)
                     /locus_tag="J8I47_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6323983.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1634 a          968 c          757 g         1782 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tcttaaaaat attctgagtt aatgattatg tatatatttt
     5041 accataatca ttaacttata tatataaact tttgaaagtc aataaaatca agggatttag
     5101 tttatgattt tatatcattt cagtaactca gaatattccg a
//
LOCUS       DADOZM010000165         5027 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.171, whole genome shotgun sequence.
ACCESSION   DADOZM010000165 DADOZM010000000
VERSION     DADOZM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.171"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(49..489)
                     /locus_tag="J8I47_002579"
     CDS             complement(49..489)
                     /locus_tag="J8I47_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ6323984.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            649..1155
                     /locus_tag="J8I47_002580"
     CDS             649..1155
                     /locus_tag="J8I47_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ6323985.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1323..3236
                     /locus_tag="J8I47_002581"
     CDS             1323..3236
                     /locus_tag="J8I47_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAZ6323986.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3241..4242
                     /locus_tag="J8I47_002582"
     CDS             3241..4242
                     /locus_tag="J8I47_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ6323987.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4444..4650)
                     /locus_tag="J8I47_002583"
     CDS             complement(4444..4650)
                     /locus_tag="J8I47_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAZ6323988.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1863 a          773 c         1008 g         1383 t
ORIGIN      
        1 caacactaga tattatagat ccagaaaagc agattgttta tgccaaaatt aagacattgc
       61 aaaaagatta ttttctttaa aatcgacaag atgaccagaa agcaagagtt cgttgagctc
      121 ttgctttctt ttagttttag aaaggaacat cctgatttta tcttcgctaa atccagtcaa
      181 aagttttgca gattggtcgt agataatcgg ttttttcaat aaagaagggg atgtacgaat
      241 ctcaatatat agttcactca aactgagttc atctattctg tgaacaagat ttttataact
      301 aatagaatga gtagagagca tttcggtcac gccgttgttt gtcaatttta gtaagtggat
      361 aaattcattt ttggttagtg gttgacgaat taagttctgt tcgataaacg gtatatcatg
      421 gacgataagc caacgttttg cgcgtcttga agacgagcag ccagaacctg tatacaattt
      481 aatcatcata cattcctcca atagctaagt atttatgaaa taataacatg aagaaatagt
      541 cgaaaagtgc catttagagg tgtaaaataa gaaaatgttg tcacgtataa tggaactagc
      601 tagataagaa atactattaa atcaagaaaa aagaaagaag gcacaagtat gtcaaacaca
      661 gccgacgcaa tagtagtgat cgatttacaa aaaggggtat gttttgattc ttcgccgata
      721 cacgaattag aaacagtgac agcattagtg aataaaagaa tcaaaacata cgaaacgatt
      781 gggaaaccag ttattttcgt tcaacacgaa gatgctcaat tagtgccaca aacaaaacca
      841 tgggagctat tgtcggaatt atctattcct gagcacagtt attttgtgag aaaaacccat
      901 gcgaattcat tttataagac taatttgagt gttttattaa ctgaattgga ctgtcattcg
      961 atcgaattat gtggggcaca agcagagtat tgtatagata cgactgtgaa gtttgctcat
     1021 ggattaggat accagcttat gatgtgtaga ggagcaacga caacttatga taatcctttt
     1081 atgaaagcaa aggaaacaat cgctttttac gagaaaattt gggataaacg atttctgaca
     1141 tttttggagc aataagttgt ttcttcgttt aaaaaaagca gaatggatac tggtttatcc
     1201 attctgcttt attacgtaaa atgagaaaat aatcctgttc tatttttatg ggataaacca
     1261 tttatttttt gtataaaaaa tgtttgcgtt tacatataat tctcctagaa acataaaaag
     1321 gagtgaatag cttgagaaca aaacaagtat taaagggagc gttggtcatc ttatttgctg
     1381 taagtacaag cgtcccgcaa tggacagtat atggagagag catttcttcc gaatcggaat
     1441 ctcgggaggt agaaaccaca gaaactactg atgctggaaa tgaaacgaaa tcgagtactt
     1501 cacaaacaga aacaaatact gaaacaacgg ttgagacatc tgttactagc acagatcaag
     1561 aaacgaccgc taccacagat aaccaacaaa cagaagaagc aacagctaca aaaatgtcaa
     1621 acgacgaatc ctcttctgct aaagtaagtg acaaagagaa tgggacagaa aatgatgata
     1681 tcggtaaagg tgaaaatttc aatggtaaag aatatttgaa ttacttcggt aaacagatga
     1741 cagaaatact taatggacca acagaagaca tcaagtcaaa attaatagag ttattagtca
     1801 ctgagcttgc tgttaatgat tatggtgtag ataaagagat tatcaaacat ctgattgcta
     1861 cttatctatt gtacgatatc gcttttgcaa aaaacagtgt gttgatgtcg atgcttggag
     1921 gtacagatga tgggacaacg cctacagaaa acacaggctt ctatccaaat acaaaacaac
     1981 ttcatcaaac tgtactaaac aaacaagatg gcaaaatgat ggatgtatat gcatattatg
     2041 tcgatcaagg ttcagataaa acagtcatga tccatggcgg atttcgaggg aattggaata
     2101 acgggatcgt tacagaagaa tacaatgatt tttataaggc cgggtataat ttattatttg
     2161 ttgattccag agcgactggt aatagcggag gcgattatgt gacctatgga caatatgagt
     2221 cagatgacgt cctttactgg ataaatcaag aagttcgaga gcggccatct caaaagattt
     2281 tactttatgg tggttcgatg ggagcagcta cgatgatgtc tgtattagcg aaagatattc
     2341 ctgtcaatgt caaaggaatt attgaaaatt gcggatttgc atcaattgat gaacaactac
     2401 gttttacgta cagccagaca gtagcccccg cactaccgga tgccataaaa aatcagttag
     2461 acatcattgg ggatcaagag catgaagatt tatttatggg ccttttgaaa caatactatt
     2521 ttgatcagga gatgcatttg gatacaaaag ctgctctacc tacgattgga atgtcagatt
     2581 cattgcctaa attgattatc catggaacag cagatgatgt agtacctgtg tctaacgcgc
     2641 aaaaattata tgagttatca ggagggtata aagatctttt attagtagaa ggagcagggc
     2701 atggaaaggc acaggaagtt gaccatgctg cctacacgaa acatgtaacg gactttttga
     2761 aagtagtgtt ccatgatcag ataaacgtaa agtatgtgga tgaaaacaat aaaagtctac
     2821 tcaaagacca ggacgaaatc cagctttacg gagcttatgg agaaaattat atgacagagc
     2881 aaaagacatt cgaaggatac gaattagcaa atgttgaagg accgactgaa gggattttta
     2941 atgagacgat accaactatt attttctatt acaaaaaaat cccagtagct ccaccaaaaa
     3001 agcaagatcc gacagagaat gcagacaata agggaaagaa tgaacctcga gaggatcaat
     3061 caagtgatga tacgcaaccg attcagatga cagtgaaagt acaaaaaaag acagatgcaa
     3121 aaaaaagaat gttgccgaaa actggagaaa agaaacattc gcttctcatt tattcaggtg
     3181 gatttttatc tgttagcacg attatcacat ggatttggcg aagaaagaga gggtaaatga
     3241 atgaaatggt tcaagcgaat agtgattgca gtaccagtta tcatcgtagt tattttagtc
     3301 actgtatttt tgattaatca aatcacacca aagccgatta gcttgcttgt tagaaaacaa
     3361 tttgacacaa gtgaaaaaga acaagtatat gcccgaccag atgatttcca gcaaaaaatc
     3421 gaacaaatcg atataaaaaa agaaatatcc tatccttcaa agtacaaaaa taataatatg
     3481 gatatctaca caccgaaagt ggaaaagaaa aaattaccga ttcttttctg gatgcatgga
     3541 ggcgcatatg taggagggga taaaaatgat tgtcgtgatt atttggaata tctatgttca
     3601 gacacgcagc aaatcatcgt aaatatagat tatgaacgat caccggaagc aaaacatcct
     3661 acaccagtta tccaattgaa cgaagccatt caagcagcta aaaaagagat aaaagatcaa
     3721 gcagattggt cgaatatttc gattggtgga aattcggcag gtgcacaaat tagtggagaa
     3781 tatttgttag cacttcaaaa tgaagaaatt aaaaaagctg atcatcttga tccgactttg
     3841 gagaacaagc agattacgaa gtttatttct ttgtctggtc tgttggatcc ttctgcactt
     3901 actcaagtaa gtgataaaat ctctagcttt ctttatgaaa aatgtggatg ggcttacttt
     3961 gataataagg actttgaaca atcagagaag gtcaaaagtt tagccttggt cagacatgcg
     4021 gatcgttgga cgcaaaacac attcttaacg gatggaaata caaatacgtt tacgaaacaa
     4081 atggaagaag taacttctgt atttgagaaa gcgggggtga aagtcacaaa agtagattat
     4141 gcggagaaaa atgctgtttt gaaccatgaa tatcagtttg atttcagcaa taaacaagcg
     4201 caagaaacat atcaaaagtt agtaatgttt ttcaaggagt aaagtcttat gatgcaaaag
     4261 aggttgtaat ctgtcactta gctccaagtc acaaggagca atttcaataa acaagctttt
     4321 catggtttat gaatattgta acttgtgact gaggagttag agatggcaag gggcaccttt
     4381 tattcgaaat ttgggaatat cacacgatta ttgtcacgat ctcttttacg taaattttat
     4441 aagtcagatt tgttgttttg atcgtttaaa aaaagcaaca aatccgaagc ctgcggaggc
     4501 aagaagggac aaaaagccga gtggtaagca aaagaatgct ggttccacta atattccgtt
     4561 gttattgata taaggctggg aacaaagatg aatagcaaat aaaatcaatg cactagcgaa
     4621 aaaaagcaaa gaaatatgga tagctttcat aagcagcacc tcctttttta taattgtatg
     4681 ataaatagat taagaatcta taaatgtgac aagtttgtta ggatgtgaat aaaatgatca
     4741 tctagcgaac ttgaaacaaa gatggctaat gataagcaat taatgattca gcaatcagat
     4801 aggtacaaag tttaccatca tacgtctaga ccaataatag aaaatatggg aatataatag
     4861 aaaagattta ggataccccc aaaagaattt ccccaaaaat tcgatgtatc ctaaactttt
     4921 ttattttaca taacaatcat atcaactgtg aaataaaatt acaaatgata acctaaaatg
     4981 aggccaaaag ccagtgttat caaggaaata cttaattcac caaaatt
//
LOCUS       DADOZM010000166         4859 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.172, whole genome shotgun sequence.
ACCESSION   DADOZM010000166 DADOZM010000000
VERSION     DADOZM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.172"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..290)
                     /locus_tag="J8I47_002584"
     CDS             complement(<1..290)
                     /locus_tag="J8I47_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAZ6323989.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="J8I47_002585"
     CDS             complement(283..624)
                     /locus_tag="J8I47_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323990.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="J8I47_002586"
     CDS             complement(798..1523)
                     /locus_tag="J8I47_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAZ6323991.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="J8I47_002587"
     CDS             1576..2052
                     /locus_tag="J8I47_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323992.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="J8I47_002588"
     CDS             complement(2058..2240)
                     /locus_tag="J8I47_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323993.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="J8I47_002589"
     CDS             complement(2240..2539)
                     /locus_tag="J8I47_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323994.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="J8I47_002590"
     CDS             complement(2644..2847)
                     /locus_tag="J8I47_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6323995.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="J8I47_002591"
     CDS             complement(2862..3608)
                     /locus_tag="J8I47_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAZ6323996.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="J8I47_002592"
     CDS             3686..3994
                     /locus_tag="J8I47_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6323997.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="J8I47_002593"
     CDS             complement(3965..4222)
                     /locus_tag="J8I47_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAZ6323998.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
BASE COUNT         1333 a          939 c          761 g         1826 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgt
//
LOCUS       DADOZM010000167         4807 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.173, whole genome shotgun sequence.
ACCESSION   DADOZM010000167 DADOZM010000000
VERSION     DADOZM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.173"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(197..1159)
                     /locus_tag="J8I47_002594"
     CDS             complement(197..1159)
                     /locus_tag="J8I47_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6323999.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1161..2600)
                     /locus_tag="J8I47_002595"
     CDS             complement(1161..2600)
                     /locus_tag="J8I47_002595"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAZ6324000.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2785..4731
                     /locus_tag="J8I47_002596"
     CDS             2785..4731
                     /locus_tag="J8I47_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAZ6324001.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1539 a          819 c          790 g         1659 t
ORIGIN      
        1 cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa aaaggagtgg
       61 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
      121 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      181 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      241 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      301 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      361 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      421 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      481 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      541 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      601 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      661 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      721 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      781 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      841 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      901 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      961 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
     1021 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1081 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1141 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1201 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1261 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1321 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1381 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1441 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1501 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1561 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1621 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1681 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1741 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1801 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1861 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1921 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1981 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     2041 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2101 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2161 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2221 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2281 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2341 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2401 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2461 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2521 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2581 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2641 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2701 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2761 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2821 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2881 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2941 acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     3001 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3061 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3121 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3181 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3241 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3301 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3361 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3421 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3481 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3541 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3601 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3661 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3721 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3781 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3841 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3901 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3961 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     4021 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4081 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4141 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4201 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4261 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4321 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4381 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4441 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4501 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4561 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4621 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4681 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4741 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4801 agaataa
//
LOCUS       DADOZM010000168         4790 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.174, whole genome shotgun sequence.
ACCESSION   DADOZM010000168 DADOZM010000000
VERSION     DADOZM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.174"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..395
                     /locus_tag="J8I47_002597"
     CDS             <1..395
                     /locus_tag="J8I47_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324002.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="J8I47_002598"
     CDS             398..1147
                     /locus_tag="J8I47_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324003.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="J8I47_002599"
     CDS             1159..1428
                     /locus_tag="J8I47_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324004.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="J8I47_002600"
     CDS             1590..1892
                     /locus_tag="J8I47_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324005.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="J8I47_002601"
     CDS             1889..2050
                     /locus_tag="J8I47_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ6324006.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="J8I47_002602"
     CDS             2047..2352
                     /locus_tag="J8I47_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324007.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2352..2663
                     /locus_tag="J8I47_002603"
     CDS             2352..2663
                     /locus_tag="J8I47_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324008.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            2830..3246
                     /locus_tag="J8I47_002604"
     CDS             2830..3246
                     /locus_tag="J8I47_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAZ6324009.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3246..3914
                     /locus_tag="J8I47_002605"
     CDS             3246..3914
                     /locus_tag="J8I47_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAZ6324010.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            3911..4288
                     /locus_tag="J8I47_002606"
     CDS             3911..4288
                     /locus_tag="J8I47_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324011.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4285..4584
                     /locus_tag="J8I47_002607"
     CDS             4285..4584
                     /locus_tag="J8I47_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324012.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
BASE COUNT         1800 a          744 c         1112 g         1134 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagaaat aatgaacgaa ctaatcacaa aagtagagca gtgggctaaa gacaagggac
     2401 tggatcaagc ggatccaaaa gcacaatttt tgaaagtagc tgaggaattc ggagaaattg
     2461 catcggcaat ggcaagaagt aatgatgagc tatttaaaga tagcgtagga gacgttatcg
     2521 tcacgctgat tattctttcc atgcaaaaag gggcaaacat acaagagtgt ttagaaatgg
     2581 cgtataacga aatcaaagga cgcacaggaa aaatggtaga tggtgtattc gtgaagtcga
     2641 gtgatttgga ggacagcgaa tgatgatgcc aaaagaaatc tattcagatg cattaagagg
     2701 tgctagaaat caattgaaaa tggcaaaaag agcatacgaa attcggccca caatcgaaaa
     2761 tgaacgcaga gtaaaagcaa ttcgccgtag atgttcaatt tacggcgagt tacagaagga
     2821 ggacagcaaa tgataccgag atttcgagct tgggataaac gaaagaacgt aatgagagat
     2881 gtagccgtct tgcattttac taaaaacggc aaaacaaact ttattgaata ttggataaat
     2941 cctaccgaat tgaaatcata tcacgtgcga aacatcgagc tcatgcaatc aacagggctg
     3001 aaagataaga atggcattga gattttcgag tgggatattg tatcagtcag cgtgcgaaat
     3061 ggattcgatt atttagataa taaagtttgt gttgtcaaaa attcaatagg acattcggga
     3121 ttagtgtgtg ccactgttga tgaagattta gagtatcaaa tttttaacac agagctgttt
     3181 gaagaataca cgtatgaagt catcggaaat atttgggaga atagcgagtt attggaggaa
     3241 cagtgatgaa taaacaggaa ttgattgatg aattagctaa atatgtaaag cgttatgaga
     3301 acgttatgga tgagcatggc caaggaaggt acggcgctta tgaagtatct ttaaagttgg
     3361 tgaaaagact aaatgaatca aaaattacgg acgaacaagc ttggaataag gtagctgagg
     3421 cttatcctga atcggcacaa agcttgagaa acactttaga taatgctgta tttggtaaga
     3481 ctggtgaacc gcagaagcac gttatgccga agtttgtggc ggagtggata gaatacgcta
     3541 aaaagaaagg tgatagtcta gctatttcat tcaagccgtg gaacctctat ggtgttgagt
     3601 atagcaaggc tgatagatgg attggagata atcaagaaac gtttgctcgt gcttggctag
     3661 acggctacga ggtcgagaaa gagccgttgt attatgtccc gctaccatat gaagtttggg
     3721 atgaagaggc agcggagctt aaaacagagt atttgtatct acattatgag atcacaagtg
     3781 atgaaactag aatatttcct acaaaagaac ctagaaaagg ttttgtcgca aaattagatg
     3841 aacttacgat taaatcagca gatgaaaact actggccgtt tgctgtacca gtggaagagg
     3901 tggtagaagg atgaaaaaat tgcaccaact catatctgag aaagaaagcg aactccaaaa
     3961 tttagaagat agtttgggat tggggtttcc aattgttgag caagttaaaa tggttcaaat
     4021 cagtcatcta caacttgaat tggaagattt aagacaaata gaagatccgt atcaactcaa
     4081 cgataaccag caaatcgtgt tggagtggtt gaaactaact gcgtcaacag gaaagccaat
     4141 gcaagttgta ttttggatga tgaataatgc agcatggggg catttagacg aattgaggga
     4201 tcctttgatg gaattaactg acaaacagca attcgaagtc ctaacagcat tcgctcaatg
     4261 gggattagaa caggaggaaa aagaatgaaa ctaaaagacg gattttacgc tagtagccac
     4321 ggtatcggcg gtttaatgct agacatgccg acaaagaacc ctaaaacacg taagaagcca
     4381 aaagtcaaag tcggtgacat ggttcgctgt gaagcagaag gattcatcta tccatttcgt
     4441 ggatatgtag aacacgtcta taatcactca gcgatcattc gcattgaaaa cacgatggaa
     4501 tgcgacaagt tattagcgaa aagcaaagat aatttagcag tggtgagatt ggtggatatg
     4561 gaggttataa acaatgaaat ttaaaatctt tgaagaggac actcgctata aattagaaaa
     4621 aaaattaaat gaatttgcaa agaataatga aattcagcat atatctttaa cggcttctaa
     4681 gtcaggttat acaacttact atgcagctgt tgtgagctac gtaattcaat aaaaggaact
     4741 cggcaaataa aaaagccgga tcactccgac tgatgtaata aatccgacaa
//
LOCUS       DADOZM010000169         4788 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.175, whole genome shotgun sequence.
ACCESSION   DADOZM010000169 DADOZM010000000
VERSION     DADOZM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.175"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            564..989
                     /locus_tag="J8I47_002608"
     CDS             564..989
                     /locus_tag="J8I47_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324013.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="J8I47_002609"
     CDS             2141..3118
                     /locus_tag="J8I47_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ6324014.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="J8I47_002610"
     CDS             3128..3625
                     /locus_tag="J8I47_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324015.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="J8I47_002611"
     CDS             4001..4282
                     /locus_tag="J8I47_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324016.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="J8I47_002612"
     CDS             4284..4700
                     /locus_tag="J8I47_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324017.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DADOZM010000170         4758 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.176, whole genome shotgun sequence.
ACCESSION   DADOZM010000170 DADOZM010000000
VERSION     DADOZM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.176"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..806
                     /locus_tag="J8I47_002613"
     CDS             <1..806
                     /locus_tag="J8I47_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6324018.1"
                     /translation="AIQVFKELALPSKIGVILNLSPTFPRSQNPADVKAAEIADLFYT
                     RSFLDPMVKGNFSKKLILLLQEYNQLPEDYTKEDLVCIYENTAQILGLNYYEPRRVKS
                     RSSMVDTSSPFLPEWFFEPHIMPGRRMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFI
                     SENGMGVADEERFMDDTGQIIDDYRIEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCW
                     SWINAYKNRYGLVSLDLTTQKRTIKKSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1105..1812
                     /locus_tag="J8I47_002614"
     CDS             1105..1812
                     /locus_tag="J8I47_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAZ6324019.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            1799..2122
                     /locus_tag="J8I47_002615"
     CDS             1799..2122
                     /locus_tag="J8I47_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAZ6324020.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2211..3248)
                     /locus_tag="J8I47_002616"
     CDS             complement(2211..3248)
                     /locus_tag="J8I47_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ6324021.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(3569..4552)
                     /locus_tag="J8I47_002617"
     CDS             complement(3569..4552)
                     /locus_tag="J8I47_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAZ6324022.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         1342 a          952 c          894 g         1570 t
ORIGIN      
        1 aagcaataca agtatttaaa gaactagctc ttcctagtaa aataggcgtt attctaaatt
       61 tgagtcctac ttttcctcgt agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg
      121 atttatttta tacgcggagc tttttagatc ctatggttaa agggaatttt tctaaaaagc
      181 ttatcctgtt attacaagaa tataatcagc ttcctgaaga ttatacgaaa gaagatcttg
      241 tatgtatcta cgaaaatacc gctcaaattc ttggactaaa ctattacgag ccacgtcgcg
      301 tcaaatctcg ttcttctatg gtggatacat ctagtccatt tttaccagag tggtttttcg
      361 aaccacatat catgcctgga cgacgaatga acgaataccg gggatgggaa atttacgaaa
      421 aaggaattta tgatttgtgc atggatatca agaaccatta tggaaacatc gaagcattta
      481 tctcggagaa cggaatgggc gttgcggatg aagaacgttt catggatgat acagggcaaa
      541 taattgatga ttatcggatt gagtatatca aagatcatct tgcttactta tggaaggcaa
      601 taaatgaagg atgtaacatt aaggggtatc atctttggac atttattgat tgctggtcat
      661 ggatcaacgc ctataaaaac cgatatgggc tggtttcact cgaccttact actcaaaaaa
      721 gaacaattaa aaaaagtggc gaattttaca agcaattaag tgatacaaat ggatttagat
      781 acgatgtaga cttgttagtt gaataatatt ctaaaattcc acttgaagtg aacacattag
      841 aaaacaagaa taatgagaaa taccaataac aaatactttt tcttcatact tcatttatac
      901 ttgtcagtat aagagactgt gacaaatgta gtgatcctca gaagttaggt ctggaaatct
      961 aacttgagaa gattttttta aactacgtcc agtctttttt atttgtttaa aattgaagtt
     1021 gacaagtctt cattaaaaat ttagaatatt cacaagtttg acgacaatta tgaaaattta
     1081 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
     1141 gacacgttac ctaccgtttt tggttatatc ggtattggac ttgcatttgg tatcgttggg
     1201 aaagctgccg ggtttcatcc gctggtcgtc acgttgatgt ccctgctgat ctatgctggt
     1261 tctgcccaat ttatcacggt cagcatgctt gctagccata gtccactgct ctccatcgtt
     1321 ctctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
     1381 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
     1441 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
     1501 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
     1561 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
     1621 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
     1681 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagt
     1741 aacttagtga ttttagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
     1801 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
     1861 taggatccta ccatttgtct tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta
     1921 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
     1981 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
     2041 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct
     2101 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
     2161 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
     2221 aacggaccac taacaacgac ttttcctaat atatgtcctg tttctacgtc tttggttgct
     2281 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
     2341 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
     2401 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
     2461 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
     2521 cggacaaggt gtttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
     2581 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
     2641 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
     2701 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccagctccg
     2761 ttgatcacta agattttttt cccgcggttg gcattttctt ctggagtcag accaaatttt
     2821 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcggc ttcttcatct
     2881 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ccagttggta ttcttgattg
     2941 cttcctgctc ttgtcgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
     3001 acttcatcac ctacagcaac aactttcccg actccatcaa accctaagat acgtaaagca
     3061 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
     3121 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
     3181 ctttttggat catcgatcga caacccttca taaaaaccga ctaccttcat taattccttt
     3241 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gttttcattt
     3301 caatatatat agtatgctat ttgttccact tttattcatc ataattattc tcttacttaa
     3361 agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac
     3421 ctcttttaaa cggtgttcaa tcaactgacc ctcggtattc gctcaaaaaa cgacaaaata
     3481 tgaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacgac
     3541 ctctaattca cggtgttcaa gcaagaaatc attgttcact gaaaagcgtt ggatgttctt
     3601 tttcaaaggt gtctaagatc tctcgtactt catcagtaga tttcgtattc atcaactgaa
     3661 ctcgcaagtc atttgctcct ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc
     3721 ctgtgatgga acgcgggatg atttctgaat aatgatcttg cagatctagt tgcagacgaa
     3781 gtagtccgat cagttctttt tctgtatgtt ccctcggttc tttttcaaat gcataaggat
     3841 tcttgaaaat cccccggcca atcatgatgc catctacgcc atatttttcg gccaattctt
     3901 ctcctgtctg gcgatctggg atatctccgt tgatcgtgat cagtgtttgc ggagcgattt
     3961 ggtcacgtaa agcaatgatt ttagggatga cttcccaatg tgcatctact ttactcatct
     4021 ctttacgcgt acgcaaatgg acagaaagat tggctatatc ttgatgaagt aggtgactga
     4081 tccattcttc catctcagac atttccgtat agccgatacg cgtcttgacg ctgacaggca
     4141 aaccgccagc tttagccgct tcaatcaatt gtgccgctac ttctgggcga aggatcaagc
     4201 cactgccttt tccacgttct gctacattag ggactgggca gcccatgttg atatcgattc
     4261 cttggaagcc catttctgcc atagcaatgc tcatttctct aaaaaattct ggcttgtctc
     4321 cccaaatatg ggcaaccatc ggctgttcat cttctgtaaa ggtcaatctt cctcgtacac
     4381 tgtcttttcc atctggatga cagaaactgt ctgagtttgt aaactcagta aagaagacat
     4441 ccggtgctgc tgcatgcttt acgacatggc gaaatacaac atctgttaca tcttccattg
     4501 gtgctaaaac aaaaaaaggc tttggcagct ctttccaaaa attagtagtc atttatttgt
     4561 tactctccat tctatcaagc agtgaagtcg caatctttct caaaaacatt gtcattatac
     4621 taggaaacaa aaaaagaagc aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa
     4681 aagactgtga caaaaatctt atcacaattc cttttccgga ataaatggtg ttcaaaagct
     4741 tgcatctgcg gtaataag
//
LOCUS       DADOZM010000171         4710 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.177, whole genome shotgun sequence.
ACCESSION   DADOZM010000171 DADOZM010000000
VERSION     DADOZM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.177"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            106..357
                     /locus_tag="J8I47_002618"
                     /pseudo
     CDS             106..357
                     /locus_tag="J8I47_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="J8I47_002619"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="J8I47_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAZ6324023.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="J8I47_002620"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="J8I47_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="J8I47_002621"
     CDS             1786..1869
                     /locus_tag="J8I47_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAZ6324024.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="J8I47_002622"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="J8I47_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAZ6324025.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="J8I47_002623"
     CDS             complement(2994..3857)
                     /locus_tag="J8I47_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAZ6324026.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="J8I47_002624"
     CDS             complement(3859..4131)
                     /locus_tag="J8I47_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ6324027.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="J8I47_002625"
     CDS             complement(4148..4363)
                     /locus_tag="J8I47_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAZ6324028.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="J8I47_002626"
     CDS             complement(4455..>4710)
                     /locus_tag="J8I47_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAZ6324029.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DADOZM010000172         4663 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.178, whole genome shotgun sequence.
ACCESSION   DADOZM010000172 DADOZM010000000
VERSION     DADOZM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.178"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            23..682
                     /locus_tag="J8I47_002627"
     CDS             23..682
                     /locus_tag="J8I47_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAZ6324030.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            701..2284
                     /locus_tag="J8I47_002628"
     CDS             701..2284
                     /locus_tag="J8I47_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAZ6324031.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2419..2997
                     /locus_tag="J8I47_002629"
     CDS             2419..2997
                     /locus_tag="J8I47_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324032.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            2943..3461
                     /locus_tag="J8I47_002630"
     CDS             2943..3461
                     /locus_tag="J8I47_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324033.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3469..4509
                     /locus_tag="J8I47_002631"
     CDS             3469..4509
                     /locus_tag="J8I47_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAZ6324034.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT         1715 a          519 c          924 g         1505 t
ORIGIN      
        1 aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa
       61 caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta
      121 tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa
      181 cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt
      241 agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc
      301 tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa
      361 aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca
      421 agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac
      481 acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt
      541 gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat
      601 tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca
      661 tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt
      721 agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac
      781 aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct
      841 cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc
      901 acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga
      961 atataatata gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc
     1021 gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg
     1081 ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc
     1141 agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg
     1201 aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa
     1261 agatatacag ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca
     1321 aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat
     1381 gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc
     1441 aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac taaatagttt
     1501 ctatcagaag agttattggg gatattcatt accaaactat atttcagcag ttcataatgc
     1561 tcatcctaat tatgcaagct atctgtcata taagaatact ttaacaattg aagcaatgaa
     1621 tgaaatattc gaaatgatgg atgaagaaaa aaaggtatct tatgataaac aatatataga
     1681 agatttatac tacaaatata tggaaactgg gaaaatacag gaggaatata tggaagaact
     1741 gagaaaattt ctctcagaca aaaaaattct actgattgca ccaggaaaaa gttctgtaga
     1801 tgaggtagaa agaataaaag agtttgttac caaagaagaa gtcgtaataa ttagtgtaaa
     1861 ttttgaatat tctcatatag atatagatta cgtattttta agtaatttaa gacgttttca
     1921 tgaattaccg aaagaaagca gaactaaatg tattgttaca tcaaatatac aagcatcgga
     1981 tgtatattta cggacgagtt ataaaaagtt gttatctgaa agggaagttg taagagacaa
     2041 tgcaggatta atggcaatta gatttttagc cgatatgaat gtggatgaaa tattcttagc
     2101 tggatttgat ggatattcac ataatgaaga tgagaattat ggtgagcaat ccatggaaat
     2161 tataacaaaa tttgcattat tagatgcaat gaacgtgcaa atgagagatg aattgtcaga
     2221 attagctaaa aaagtaaagg tgacattttt aacggatcca cgttgggttc gaattgataa
     2281 gtagtagtaa taatgttttt aaaattttga taaagagata gtgagagaat aaaaatgggt
     2341 aaagtcttaa ataaaaaaga agaaaaatat ttaagggacg atggggcgag atgaaactct
     2401 aattttgaac ttttaagaat catttctata ttgtgtattg ttgcttacca tgctaatttg
     2461 cgtttaaatt ataatgcaga cggaattata gtacatggtt cattaactgc taaccagatt
     2521 ttcttgaatt atattggtat ttggggtcag ctaggagtta attgttttat atttatttcg
     2581 gcatattttt taagtgagaa aagaaacatt cactttgaaa atgttttggg aattgtatta
     2641 gaatgtattt tttatagtgt attagtttat ggaattcttt gtgcatgtgg aatagaaaca
     2701 ttttcattga acagctttgt aaaagaaata ttatcatttg ctctaggtag atattggttt
     2761 attacatcct atttagcatt gtatattcta agtccgtatt taaatatcat gcttaacaat
     2821 ctggaaaaac ttcagttgca aaaaataagt atgttattgt ttataggtgt atgctgttat
     2881 cgtatgttat ttaaaacatc tttgtttggg gattgtgctg ttttaattgc actatatgtc
     2941 atatgttata cactgaaaaa aacgataata atatttttga gagacacaga atcataggaa
     3001 caattgtatg tataacagtt ataccacttc tcatatccat aggaatactt ttcaaattta
     3061 taccaaatgc aatggttaac cagatggttc atattggttc tccattgatg gttatagcaa
     3121 gtatattttt attttatgta tttaaaaatt tttcatttta cagtaagtat ataaataaac
     3181 ttgcgagaac cagtttaggg gtatatattt tacatgaaaa tcattatttc attgatggta
     3241 tgaagtctag aggcttaata tgggatggga tatttggatt aggcaaaagg tttttagata
     3301 gggatttttg gatttggttt ataattgcag ttgtagttgt gtgcataatt tgcatggcta
     3361 tcgatgttgt aagagaaaaa tttgaaagac agatttttaa aattaagagt gtaaagaacc
     3421 tgtgtaaact tatgaatcat gttataaata tatacccata attaataaat gaggagaaaa
     3481 gaagtgaaag ttatatcatt cggagaatta ttattgagac ttgcagcacc cggttattca
     3541 aagttatttc aaagagatag tttggagact acattctgcg gaggggaagc taatgtagcg
     3601 gtatccttag caatatttgg atatcaatca gaatttataa caaagcttcc ggattcagat
     3661 gttggatatg cggcagctaa tgctttgcgt tattttggag taaatgttga taatattata
     3721 tttggagagg gacgcatggg actgtattat ttagaaaaag gagcagctca gagaccatct
     3781 aaagtaattt atgacagaaa attttctgca atttcgttag ctgatagtga tgaatttaag
     3841 tggaaggaga tcctaaacga tgcaaattgg tttcattgga cagggataaa tccagcattg
     3901 tctgagaatc tctttgagac gtccttagat gcatgtcagg ctgccaaaaa aagaggtata
     3961 acaatttctt gtgatttaaa ttatcgatcc aagctttgga catcagagga agcaaaagaa
     4021 aagatggaac agatactacc attggttgat atttgtattt gtaatgaaga agatgcagaa
     4081 aaagtgctag gaattaaagc tgagcataca aatattaata gtggagaatt aaataaagat
     4141 ggctatgtgc aggtggctag ggagattcac aagaagtttg gatgtaggta tgtagctaca
     4201 actctgcgta gaagttattc ggcaagtaga aatggttgga gtgcaatgtt atacgaagca
     4261 gaaaaagata aagcgtattt ttccacagaa tacaacatac aaattgttga tcgcgttggt
     4321 ggtggagaca gcttttctgc tggtataata tatgcgttat gtgaaaatat gtccggacaa
     4381 aaggctattg aatttgcgac tgcatctagt tgtttaaaac atactataga aggtgatttt
     4441 aatcgaacta caattgagga tgtatataat ttaatgaata gcggtggaaa tggtcgcgtg
     4501 ttgcgttagt caacatataa agataaatca cagtaatttt tatggtgatt atctgtctta
     4561 gtagtgatta gaataacgtg aaaataaatt gttgagcgtt tatactgttt tgaaaaataa
     4621 atttaacata ttgaaaaata caaatatata cgtatattat aaa
//
LOCUS       DADOZM010000173         4632 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.179, whole genome shotgun sequence.
ACCESSION   DADOZM010000173 DADOZM010000000
VERSION     DADOZM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.179"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            202..1077
                     /locus_tag="J8I47_002632"
     CDS             202..1077
                     /locus_tag="J8I47_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324035.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="J8I47_002633"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="J8I47_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="J8I47_002634"
     CDS             complement(1642..2973)
                     /locus_tag="J8I47_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6324036.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="J8I47_002635"
     CDS             complement(3122..3835)
                     /locus_tag="J8I47_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ6324037.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..>4632
                     /locus_tag="J8I47_002636"
     CDS             4010..>4632
                     /locus_tag="J8I47_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ6324038.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKA"
BASE COUNT         1602 a          852 c          707 g         1471 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gc
//
LOCUS       DADOZM010000174         4611 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.180, whole genome shotgun sequence.
ACCESSION   DADOZM010000174 DADOZM010000000
VERSION     DADOZM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.180"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(86..340)
                     /locus_tag="J8I47_002637"
     CDS             complement(86..340)
                     /locus_tag="J8I47_002637"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ6324039.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="J8I47_002638"
     CDS             complement(451..705)
                     /locus_tag="J8I47_002638"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6324040.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="J8I47_002639"
     CDS             complement(898..1173)
                     /locus_tag="J8I47_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAZ6324041.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="J8I47_002640"
     CDS             complement(1145..2098)
                     /locus_tag="J8I47_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ6324042.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="J8I47_002641"
     CDS             2710..4203
                     /locus_tag="J8I47_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAZ6324043.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="J8I47_002642"
     CDS             4338..>4611
                     /locus_tag="J8I47_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAZ6324044.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DADOZM010000175         4483 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.181, whole genome shotgun sequence.
ACCESSION   DADOZM010000175 DADOZM010000000
VERSION     DADOZM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.181"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..1475
                     /locus_tag="J8I47_002643"
     CDS             138..1475
                     /locus_tag="J8I47_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAZ6324045.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="J8I47_002644"
     CDS             1523..3652
                     /locus_tag="J8I47_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAZ6324046.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="J8I47_002645"
     CDS             3627..4316
                     /locus_tag="J8I47_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324047.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         1391 a          799 c         1010 g         1283 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaa
//
LOCUS       DADOZM010000176         4475 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.182, whole genome shotgun sequence.
ACCESSION   DADOZM010000176 DADOZM010000000
VERSION     DADOZM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.182"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            323..1054
                     /locus_tag="J8I47_002646"
     CDS             323..1054
                     /locus_tag="J8I47_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ6324048.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="J8I47_002647"
     CDS             1199..3226
                     /locus_tag="J8I47_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAZ6324049.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="J8I47_002648"
     CDS             3334..4431
                     /locus_tag="J8I47_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAZ6324050.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DADOZM010000177         4468 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.183, whole genome shotgun sequence.
ACCESSION   DADOZM010000177 DADOZM010000000
VERSION     DADOZM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.183"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(279..809)
                     /locus_tag="J8I47_002649"
     CDS             complement(279..809)
                     /locus_tag="J8I47_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAZ6324051.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            914..1465
                     /locus_tag="J8I47_002650"
     CDS             914..1465
                     /locus_tag="J8I47_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ6324052.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1618..2091)
                     /locus_tag="J8I47_002651"
     CDS             complement(1618..2091)
                     /locus_tag="J8I47_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ6324053.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2229..3701
                     /locus_tag="J8I47_002652"
     CDS             2229..3701
                     /locus_tag="J8I47_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ6324054.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3698..4408
                     /locus_tag="J8I47_002653"
     CDS             3698..4408
                     /locus_tag="J8I47_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAZ6324055.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1406 a          815 c          863 g         1384 t
ORIGIN      
        1 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
       61 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      121 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      181 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      241 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      301 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      361 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      421 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      481 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      541 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      601 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      661 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      721 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      781 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      841 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      901 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
      961 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1021 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1081 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1141 agaagaaggg aaggaacaat tggcagagtg gattcataca ccaaccaatg aactaccggt
     1201 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1261 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1321 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1381 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1441 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1501 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1561 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1621 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1681 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1741 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1801 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1861 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1921 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     1981 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2041 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2101 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2161 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2221 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2281 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2341 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2401 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2461 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2521 ttacttgctt tacagcacct aattttagta cattactaat cggtcgcttg atccaagctt
     2581 gcggcgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2641 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2701 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2761 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2821 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2881 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     2941 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3001 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3061 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3121 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3181 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3241 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3301 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3361 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3421 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3481 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3541 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3601 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3661 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3721 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3781 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3841 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3901 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     3961 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4021 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4081 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4141 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4201 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4261 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4321 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4381 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgtaggag gttgtgaaaa
     4441 agctgctctg catcaagcag taagaaca
//
LOCUS       DADOZM010000178         4433 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.184, whole genome shotgun sequence.
ACCESSION   DADOZM010000178 DADOZM010000000
VERSION     DADOZM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.184"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="J8I47_002654"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="J8I47_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAZ6324056.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="J8I47_002655"
     CDS             complement(2944..3378)
                     /locus_tag="J8I47_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324057.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4433)
                     /locus_tag="J8I47_002656"
     CDS             complement(3395..>4433)
                     /locus_tag="J8I47_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAZ6324058.1"
                     /translation="KDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMN
                     NLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLS
                     KTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSG
                     DTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSG
                     IVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNE
                     MHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQ
                     LGLVDNFNNIS"
BASE COUNT         1374 a          824 c          611 g         1624 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct tta
//
LOCUS       DADOZM010000179         4433 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.185, whole genome shotgun sequence.
ACCESSION   DADOZM010000179 DADOZM010000000
VERSION     DADOZM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.185"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(543..2048)
                     /locus_tag="J8I47_002657"
     CDS             complement(543..2048)
                     /locus_tag="J8I47_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6324059.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="J8I47_002658"
     CDS             complement(2045..2731)
                     /locus_tag="J8I47_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6324060.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="J8I47_002659"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="J8I47_002659"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAZ6324061.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DADOZM010000180         4301 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.186, whole genome shotgun sequence.
ACCESSION   DADOZM010000180 DADOZM010000000
VERSION     DADOZM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.186"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1097)
                     /locus_tag="J8I47_002660"
     CDS             complement(<1..1097)
                     /locus_tag="J8I47_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6324062.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="J8I47_002661"
     CDS             complement(1227..2603)
                     /locus_tag="J8I47_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6324063.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="J8I47_002662"
     CDS             complement(2603..3259)
                     /locus_tag="J8I47_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6324064.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>4301)
                     /locus_tag="J8I47_002663"
     CDS             complement(3269..>4301)
                     /locus_tag="J8I47_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6324065.1"
                     /translation="IIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTK
                     RNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIK
                     SHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSG
                     SAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMV
                     VLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLK
                     VIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKK
                     PKEILSKIS"
BASE COUNT         1346 a          784 c          534 g         1637 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat t
//
LOCUS       DADOZM010000181         4219 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.187, whole genome shotgun sequence.
ACCESSION   DADOZM010000181 DADOZM010000000
VERSION     DADOZM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.187"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..1257)
                     /locus_tag="J8I47_002664"
     CDS             complement(37..1257)
                     /locus_tag="J8I47_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ6324066.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="J8I47_002665"
     CDS             complement(1277..1882)
                     /locus_tag="J8I47_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ6324067.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="J8I47_002666"
     CDS             complement(1931..2863)
                     /locus_tag="J8I47_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAZ6324068.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="J8I47_002667"
     CDS             complement(3284..4144)
                     /locus_tag="J8I47_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324069.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1198 a          853 c          690 g         1478 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgctt
//
LOCUS       DADOZM010000182         4190 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.188, whole genome shotgun sequence.
ACCESSION   DADOZM010000182 DADOZM010000000
VERSION     DADOZM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.188"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            84..1385
                     /locus_tag="J8I47_002668"
     CDS             84..1385
                     /locus_tag="J8I47_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ6324070.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1412..1672
                     /locus_tag="J8I47_002669"
     CDS             1412..1672
                     /locus_tag="J8I47_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAZ6324071.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2671..3906)
                     /locus_tag="J8I47_002670"
     CDS             complement(2671..3906)
                     /locus_tag="J8I47_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAZ6324072.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1211 a          670 c          867 g         1442 t
ORIGIN      
        1 cttaattata taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat
       61 tttattgaaa aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg
      121 tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg
      181 tttcaacatt gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt
      241 ttcctggatg gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga
      301 tgacgatggg attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat
      361 catatggatt agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac
      421 aagttcctgt aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga
      481 gttctttggg agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag
      541 tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg
      601 ctgttgcccg tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc
      661 tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt
      721 taaccaatat tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt
      781 tgttgtggtc atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt
      841 ggctgatcat gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat
      901 taccctatat cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag
      961 caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg
     1021 ttggtcgttt ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag
     1081 ctcctatgat gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga
     1141 ctattatcag ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa
     1201 catggacatt accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg
     1261 ttatcttgat catgatcaac acattgatag catttattat ctattatcca tttgtaaaag
     1321 cttatgataa aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg
     1381 tctaagtttg gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt
     1441 tatcttttta ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca
     1501 aatcaatcga tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat
     1561 tgtaccgcat atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc
     1621 tatcaatttt atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta
     1681 aaagtggttg taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc
     1741 ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga
     1801 tgcaagcttt tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag
     1861 ccctttttta cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa
     1921 aatggaaatt ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag
     1981 tcaaatatag atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat
     2041 ggtaatatgt atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg
     2101 ctgggaatag caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata
     2161 gaacattctg acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat
     2221 gcatcggatt gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag
     2281 tggtaaatcc tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca
     2341 aagcgatgct tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat
     2401 ggtctttctt ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg
     2461 actaagaata aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt
     2521 taaggatgat tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca
     2581 ctatttactt tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt
     2641 ctctattaaa aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc
     2701 gatttcttga attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc
     2761 ctgctgacga atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt
     2821 cactggtttt gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct
     2881 ttgctcaaat tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac
     2941 ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga
     3001 cagatattct gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc
     3061 aataggtgca ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa
     3121 catacgcgga ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg
     3181 ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat
     3241 tggaccaacg attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt
     3301 tgtgatgaga tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat
     3361 ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt
     3421 ttttgtccag tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat
     3481 cagcgcatgc tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa
     3541 aggaatcttt ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag
     3601 tcgaggctca aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc
     3661 tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg
     3721 ttttttcgta aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg
     3781 agcaaaagtt ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc
     3841 aacctcaatc attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt
     3901 cttcatgaaa tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa
     3961 aaaacacagc gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt
     4021 tcacaacttc taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac
     4081 gcaacaattt tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt
     4141 aaaaataaac atgagaacat aaaaaaataa aataattata ttaattgata
//
LOCUS       DADOZM010000183         4138 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.189, whole genome shotgun sequence.
ACCESSION   DADOZM010000183 DADOZM010000000
VERSION     DADOZM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.189"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(22..996)
                     /locus_tag="J8I47_002671"
     CDS             complement(22..996)
                     /locus_tag="J8I47_002671"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAZ6324073.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="J8I47_002672"
     CDS             complement(1076..1951)
                     /locus_tag="J8I47_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAZ6324074.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="J8I47_002673"
     CDS             2081..3988
                     /locus_tag="J8I47_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAZ6324075.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         1269 a          851 c          779 g         1239 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttc
//
LOCUS       DADOZM010000184         4046 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.190, whole genome shotgun sequence.
ACCESSION   DADOZM010000184 DADOZM010000000
VERSION     DADOZM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.190"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..938)
                     /locus_tag="J8I47_002674"
     CDS             complement(<1..938)
                     /locus_tag="J8I47_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAZ6324076.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="J8I47_002675"
     CDS             complement(1160..1522)
                     /locus_tag="J8I47_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324077.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1834)
                     /locus_tag="J8I47_002676"
     CDS             complement(1526..1834)
                     /locus_tag="J8I47_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324078.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1843..2262)
                     /locus_tag="J8I47_002677"
     CDS             complement(1843..2262)
                     /locus_tag="J8I47_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324079.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="J8I47_002678"
     CDS             complement(2255..2488)
                     /locus_tag="J8I47_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324080.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="J8I47_002679"
     CDS             complement(2491..3459)
                     /locus_tag="J8I47_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAZ6324081.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1151 a          909 c          556 g         1430 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaatac ttctaattcc tcagaaagcc ccatatcctt
     4021 ttctgcaaga ccaaaacgga tagaca
//
LOCUS       DADOZM010000185         4036 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.191, whole genome shotgun sequence.
ACCESSION   DADOZM010000185 DADOZM010000000
VERSION     DADOZM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.191"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            853..1065
                     /locus_tag="J8I47_002680"
     CDS             853..1065
                     /locus_tag="J8I47_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324082.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="J8I47_002681"
     CDS             complement(1343..3142)
                     /locus_tag="J8I47_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAZ6324083.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="J8I47_002682"
     CDS             complement(3266..3862)
                     /locus_tag="J8I47_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ6324084.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DADOZM010000186         3976 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.192, whole genome shotgun sequence.
ACCESSION   DADOZM010000186 DADOZM010000000
VERSION     DADOZM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.192"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(18..1484)
                     /locus_tag="J8I47_002683"
     CDS             complement(18..1484)
                     /locus_tag="J8I47_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ6324085.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="J8I47_002684"
     CDS             complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="J8I47_002684"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAZ6324086.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1203 a          846 c          633 g         1294 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttttttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac gattggtgtg
      121 atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaaaat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgcccct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc tttacatttt
     1741 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt tttacatgca
     1801 agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca aatactgcat
     1861 ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt atcccaatag
     1921 caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg attgcttctg
     1981 caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc
     2041 ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt gcatcgttga
     2101 ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc tctaaccgct
     2161 gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact tgcttcccga
     2221 tatgatcagc aatttttttc gagtcgcccg tcaacataac tgtgttcttc acaccagcct
     2281 gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca ataacaagat
     2341 aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct tgacttaaag
     2401 gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact tgtttgccat
     2461 caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt ggtaaaagcg
     2521 gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac tgttctgcac
     2581 tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc tggatcgaaa
     2641 agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt tgcgcaagca
     2701 actccaagta atttgaacct tttactaata caccgatttt acttgcgcct ccgataccgc
     2761 caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc actaaaaatg
     2821 tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa
     2881 cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg aatttagtaa
     2941 tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct aataatttgc
     3001 tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta tttttattga
     3061 taaatccgct taatactgtt tcaccaacat atacagattt agggactgat tctcctgtaa
     3121 gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac ggtacacgtt
     3181 caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc acggtcatca
     3241 atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat tcgcgaatcg
     3301 attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga taaaatagca
     3361 tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta gcaacagaca
     3421 taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc ttcttcacta
     3481 catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt ggttcattaa
     3541 aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg aacagcgtat
     3601 ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct gttactttga
     3661 catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta gccaaatgat
     3721 cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct tccttgactc
     3781 cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa tctaaacctt
     3841 ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac atctcttcat
     3901 aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa
     3961 catctgcatc ggaaaa
//
LOCUS       DADOZM010000187         3925 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.193, whole genome shotgun sequence.
ACCESSION   DADOZM010000187 DADOZM010000000
VERSION     DADOZM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.193"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(573..1130)
                     /locus_tag="J8I47_002685"
     CDS             complement(573..1130)
                     /locus_tag="J8I47_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324087.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="J8I47_002686"
     CDS             complement(1309..1920)
                     /locus_tag="J8I47_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324088.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="J8I47_002687"
     CDS             complement(2033..2290)
                     /locus_tag="J8I47_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324089.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="J8I47_002688"
     CDS             complement(2887..3165)
                     /locus_tag="J8I47_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324090.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="J8I47_002689"
     CDS             complement(3199..3450)
                     /locus_tag="J8I47_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324091.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DADOZM010000188         3879 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.194, whole genome shotgun sequence.
ACCESSION   DADOZM010000188 DADOZM010000000
VERSION     DADOZM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.194"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            414..1103
                     /locus_tag="J8I47_002690"
     CDS             414..1103
                     /locus_tag="J8I47_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6324092.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1090..2298
                     /locus_tag="J8I47_002691"
     CDS             1090..2298
                     /locus_tag="J8I47_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ6324093.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2592..3863
                     /gene="serS"
                     /locus_tag="J8I47_002692"
     CDS             2592..3863
                     /gene="serS"
                     /locus_tag="J8I47_002692"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAZ6324094.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1274 a          670 c          781 g         1154 t
ORIGIN      
        1 ttcgatagaa tcaagcttcg caagtggttc ttttttaacg actcagttat cttatcatgt
       61 catttccaac atgtcaacaa cttatttttt attatttaat aagttattgg tgttcttttg
      121 aacaaataat aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca
      181 acatttctag cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac
      241 tccgagagac gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac
      301 accaactaga tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt
      361 ctgctttttt ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa
      421 ttttgattgc tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg
      481 aggggtatga agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa
      541 tccctgatat tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag
      601 tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta
      661 ctgatatgga taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat
      721 ttaatccgtt agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga
      781 ttgccccaga tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc
      841 atgaagttaa aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc
      901 tctacttgct tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg
      961 tttggaaaca agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa
     1021 gagataagat tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg
     1081 gctataaaat tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg
     1141 acttcaaaag aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt
     1201 ctattaaatg tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg
     1261 acgaatgcga tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc
     1321 tggaacggcc ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat
     1381 tggcgcttga ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac
     1441 tacatcgcaa acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc
     1501 cgagtggtaa caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa
     1561 cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc
     1621 ccactcacgt ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa
     1681 gaagatcttt taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta
     1741 gtggacgact tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct
     1801 gcctttgaca tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct
     1861 aaaaaaaata tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat
     1921 accgaaaaac ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa
     1981 ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc
     2041 aaaaataacg gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat
     2101 cgtgtagaag aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct
     2161 caaagcattg tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact
     2221 tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa
     2281 gttattgacg aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa
     2341 acacagaacc cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca
     2401 aaggataatt tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt
     2461 caacagccgt tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat
     2521 ttcagactga ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag
     2581 gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa
     2641 aattgatgac tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa
     2701 atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg
     2761 ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga
     2821 aagaagtcgg tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat
     2881 tgaaacaaat tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag
     2941 atgaagagga caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac
     3001 caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa
     3061 aagtagccgg tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg
     3121 tctacaactt catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc
     3181 cttatatcgt caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag
     3241 atgttttcca attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa
     3301 ctaactatta taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac
     3361 taagccctgc ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc
     3421 gtcttcacca attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg
     3481 aagaattgga aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt
     3541 atcgtgtgat ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact
     3601 tagaagtatg gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg
     3661 aagatttcca agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact
     3721 atactcatac tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg
     3781 aaaactacca aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg
     3841 ggaatctgaa agttattaaa taaagaggct gtgaaaaag
//
LOCUS       DADOZM010000189         3713 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.195, whole genome shotgun sequence.
ACCESSION   DADOZM010000189 DADOZM010000000
VERSION     DADOZM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.195"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="J8I47_002693"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="J8I47_002693"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAZ6324095.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="J8I47_002694"
     CDS             complement(2261..2512)
                     /locus_tag="J8I47_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAZ6324096.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="J8I47_002695"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="J8I47_002695"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAZ6324097.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="J8I47_002696"
     CDS             3462..3680
                     /locus_tag="J8I47_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324098.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DADOZM010000190         3705 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.196, whole genome shotgun sequence.
ACCESSION   DADOZM010000190 DADOZM010000000
VERSION     DADOZM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.196"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            75..470
                     /locus_tag="J8I47_002697"
                     /pseudo
     CDS             75..470
                     /locus_tag="J8I47_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="J8I47_002698"
     CDS             790..1104
                     /locus_tag="J8I47_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324099.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="J8I47_002699"
     CDS             1208..2386
                     /locus_tag="J8I47_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6324100.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="J8I47_002700"
     CDS             2789..3082
                     /locus_tag="J8I47_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAZ6324101.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1266 a          548 c          717 g         1174 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatac
//
LOCUS       DADOZM010000191         3612 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.197, whole genome shotgun sequence.
ACCESSION   DADOZM010000191 DADOZM010000000
VERSION     DADOZM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.197"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            200..2389
                     /locus_tag="J8I47_002701"
     CDS             200..2389
                     /locus_tag="J8I47_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6324102.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2386..>3612
                     /locus_tag="J8I47_002702"
     CDS             2386..>3612
                     /locus_tag="J8I47_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ6324103.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1366 a          492 c          604 g         1150 t
ORIGIN      
        1 cataaaggtt tccaacaggc cgaaggcgag ctcgaacaaa tgctaagcca actgattgag
       61 aattagaaac aatctgcagt agggaagaat catttacaca aaattattga cgctccctta
      121 taatgagaac ttttactaac ataatttatg ataaacttac tatataatca tattaattaa
      181 atagttagga gatctgctta tgtttatatc tactcttcaa attcgaaatt ttagaaatta
      241 tgaatccgaa atctttcgat tcgataatga gacaaatact attataggtg agaatgattc
      301 agggaaaacc aatgctctta ctgcattgag aatcttatta gatgatagtt attactatag
      361 tagtaaaaca ctaaaagaat cagatttttt tcatggaatt caaaatgggt ggcaagggca
      421 ttggattatt atctctgcga catttgaagg aatcagcgaa gaagaatttg acaatgagat
      481 ttgtgcttcc atttctttaa attctgaaag tcaaacaatc ttagaagaat tgatttcaaa
      541 tgcagataaa ggtgtaggta gtatttctct atttattaga cctaacaaag ctattagaaa
      601 gcaattattt gatatttcca ctgaattaga ctctcatcaa tttaacgaat ttagaaattc
      661 tattcgtcta tccgactatg aattttacta cacatctaaa tcaaatctaa atttctgtat
      721 caatgaaaat tatgataaat tggtaggaag actcaatgac aatatagcta gtaatcctga
      781 agaagacgat gaagctctgt tgggctcaag aattgacatg tcagatattt tcaagcatat
      841 ttcggttgtt tatgttgacg ctcttcgaga tgttcttaga gaaatgaaaa acaatcgaaa
      901 cccagtcaaa agaatcatgg aaacaattga atcaaaaata tcatcagata atgttgagtc
      961 attaaaaaca attatacagc aattaaatga gactattacc agtgttcctg aaattaggaa
     1021 aattggcgaa aatattaatc ggcaactaaa tagcataatt ggttcagtgt attctcctaa
     1081 tcttcttctt tcctcaacta tgtcagatga tatgggttct ttagcaaaat ttctttcaat
     1141 gaagccagaa caaaatatcg acttagactt actagggtta ggacatctaa atatgattta
     1201 tttagctttg aaaatagtcg aatttgaagc gtgtcgttca agagaactac ttaatattat
     1261 gttgattgaa gaaccagaag ctcatattca tcatcatatt caaaaaactc tgttcgaagg
     1321 attaaatctt caaaaaaact atactcagat tctcatgacc acacattctg tacatttagc
     1381 tgaagtgtct gaaatatctc ggatgaatgt attaaaatcg tttaatggca aatctatcgt
     1441 tatgaatcca gcaaataatc tgaactcatt tgctaaagaa aagttaaaaa agaacaatct
     1501 aaatctaata aacgcagtag agagatattt agatgttaaa agaaatggat tgcttttttc
     1561 taaaggtgtt atcttagttg aaggagatgc tgaagaaata cttattcctc aaatagtaaa
     1621 aaaattattt ggtataaact tagacgaact tggaataggt ttgattaatg ttggtagtac
     1681 ttcgtttgaa tatatcgcta gcttgtttga cgatgataga atacagagaa agtgtgctat
     1741 tgtaactgat ttagatgaac aggcaatccc atcagaacat cgattgtata aaagcactgc
     1801 acaagagaaa ggtcaagcaa gaaaagaaaa attatcctcc ctttttaatg aaaacaattg
     1861 ggttaattgt ttttatgcta atacaacatt tgaaattgag tttttagatg tgaataaaga
     1921 aaatattcta acttatgtca gtccaattat agatgacgca ttcaaagata aaaaaacaat
     1981 aactgcttat aaagaagaaa tacaaagtga acaatattta gaaagaaatt tagcgatgtt
     2041 aaaactagcc aatcatgttg gtaaggggtg gtttgcaatt gagttaggtg aaaaaattga
     2101 ctttttaatc aaaattccta agtatattct taatgctatt gcttttgcat gtcaggaaat
     2161 agtagatctc tcaatttata aaaaaattct actccataca atcgacttgt acgaggaaac
     2221 tacagaattg tcagctttat cagctagttt aaaaacaagt gatacagctg atttgaatat
     2281 agcaataatt tctttattag agtactttgg taaagatgag gatgttgtgt atctaatcaa
     2341 tcagatagaa aattacacaa aaatacttta tgctgaggtt tcaaaatgac aaatatcttc
     2401 gaaaaaatcg taaatgattt aaatttagaa caaaaagatg ttgtggaaag tgaagataac
     2461 ctttatgtaa atgcatgtcc tgggagtggg aaaacaaggg tacttacaag gaaaattgct
     2521 taccaagcaa ttaaacacga tggatcacta aaaagaataa ttgctatcac atacactaat
     2581 agagctgctg aagaaattaa ggaacgccta agtttactta gtatagatga tgatattaat
     2641 atttgggtgg ggacaataca tcagttttgt cttgaattta ttatttatcc ttttaaaatg
     2701 aatctctcaa gagtatcaaa aggatttaca attattgatg attatactca aagagcttac
     2761 gtcagccaaa ttaataatat gctgaattta aatctcaaaa cgtatgaact gaacaaaatt
     2821 gatttgtcac tttctcatga ttttaaaatt aatgaagttc agtatactca agtttctgaa
     2881 atgtatcaca ctatgttgga aaaaaataaa gaaattgatt ttgatatgat tttactagta
     2941 gcgtataaaa ttttaactgt aaacagtaac gttgcacgta atattgctag taatataagg
     3001 gcgatttatg tggatgaatt tcaagatact agagaattac aatacaatgt aatagctaaa
     3061 ttacttcaaa acaaccctca aattcagtca atgtttgttg gagatattga ccaagcaatt
     3121 tatggatcat tagatggtat agctaaatca gttgaagaac tagaaatgct aactagtcac
     3181 acttttcaat caaagacgct acatggctgt tacaggtcta atcagagatt agtagacttc
     3241 tactctaatt ttcagagttg tccttacgag attgaaagca gagggaataa taaaaacgac
     3301 cgtggtttta taagttatga ttgcaaagtt actaaggagg atttacccaa cataataaaa
     3361 aatataataa aggaaaagat tgaaagcggc attcatgaaa aagatatttg tgtacttgct
     3421 cctcaacact atcctctgtt ttcactgggt gagtatttaa agaaagaatt accgtattgc
     3481 aattttgatg ctataaatat atcaccaatt aaggtaaata atcatagttt atttttcaag
     3541 ttagcaattt tgtactttac tgaatcaggt gaaaaggttc gaagaagaaa gctaattgca
     3601 aacgatattt ta
//
LOCUS       DADOZM010000192         3582 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.198, whole genome shotgun sequence.
ACCESSION   DADOZM010000192 DADOZM010000000
VERSION     DADOZM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.198"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            798..1064
                     /locus_tag="J8I47_002703"
     CDS             798..1064
                     /locus_tag="J8I47_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324104.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="J8I47_002704"
     CDS             1122..1634
                     /locus_tag="J8I47_002704"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6324105.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="J8I47_002705"
     CDS             1631..2668
                     /locus_tag="J8I47_002705"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ6324106.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2828..2917
                     /locus_tag="J8I47_002706"
                     /pseudo
     CDS             2828..2917
                     /locus_tag="J8I47_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2918..>3582
                     /gene="aac(6')-Ie"
                     /locus_tag="J8I47_002707"
     CDS             2918..>3582
                     /gene="aac(6')-Ie"
                     /locus_tag="J8I47_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAZ6324107.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT         1335 a          552 c          705 g          990 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgct tgccatgtat atgaaaaatt aggtggtaaa aatgattata aagatgaaat
     2881 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
     2941 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
     3001 aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa
     3061 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
     3121 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
     3181 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
     3241 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
     3301 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
     3361 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
     3421 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
     3481 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
     3541 cggtagtggt tatgatagtg tggcatattt agttaataat ga
//
LOCUS       DADOZM010000193         3581 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.199, whole genome shotgun sequence.
ACCESSION   DADOZM010000193 DADOZM010000000
VERSION     DADOZM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.199"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(27..878)
                     /locus_tag="J8I47_002708"
     CDS             complement(27..878)
                     /locus_tag="J8I47_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6324108.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="J8I47_002709"
     CDS             1001..1468
                     /locus_tag="J8I47_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAZ6324109.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="J8I47_002710"
     CDS             complement(1731..2213)
                     /locus_tag="J8I47_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ6324110.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="J8I47_002711"
     CDS             complement(2313..3176)
                     /locus_tag="J8I47_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ6324111.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1042 a          771 c          565 g         1203 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa a
//
LOCUS       DADOZM010000194         3559 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.200, whole genome shotgun sequence.
ACCESSION   DADOZM010000194 DADOZM010000000
VERSION     DADOZM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.200"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            4..1194
                     /locus_tag="J8I47_002712"
     CDS             4..1194
                     /locus_tag="J8I47_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAZ6324112.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1298..2053
                     /locus_tag="J8I47_002713"
     CDS             1298..2053
                     /locus_tag="J8I47_002713"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAZ6324113.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2169..3467
                     /gene="eno"
                     /locus_tag="J8I47_002714"
     CDS             2169..3467
                     /gene="eno"
                     /locus_tag="J8I47_002714"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAZ6324114.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         1239 a          675 c          747 g          898 t
ORIGIN      
        1 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
       61 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
      121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
      181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
      241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
      301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
      361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
      421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
      481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
      541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
      601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
      661 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
      721 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
      781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
      841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
      901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
      961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
     1021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca
     1081 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg
     1141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
     1201 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
     1261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
     1321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
     1381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
     1441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
     1501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
     1561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
     1621 catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgtgg
     1681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
     1741 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
     1801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
     1861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
     1921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
     1981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga
     2041 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
     2101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
     2161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
     2221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
     2281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
     2341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
     2401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
     2461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
     2521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
     2581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
     2641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
     2701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
     2761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
     2821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
     2881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
     2941 tagctgattc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
     3001 tagtttctaa atatccaatc atctcaatcg aagacggatt agacgaaaac gactgggacg
     3061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
     3121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
     3181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
     3241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
     3301 tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta
     3361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
     3421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
     3481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
     3541 ctctctactt gctgaaata
//
LOCUS       DADOZM010000195         3530 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.201, whole genome shotgun sequence.
ACCESSION   DADOZM010000195 DADOZM010000000
VERSION     DADOZM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.201"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..2232)
                     /locus_tag="J8I47_002715"
     CDS             complement(<1..2232)
                     /locus_tag="J8I47_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324115.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="J8I47_002716"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="J8I47_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1000 a          779 c          550 g         1201 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa
//
LOCUS       DADOZM010000196         3478 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.202, whole genome shotgun sequence.
ACCESSION   DADOZM010000196 DADOZM010000000
VERSION     DADOZM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.202"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(766..1722)
                     /locus_tag="J8I47_002717"
     CDS             complement(766..1722)
                     /locus_tag="J8I47_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324116.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1831..2076)
                     /locus_tag="J8I47_002718"
                     /pseudo
     CDS             complement(1831..2076)
                     /locus_tag="J8I47_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2221..2643)
                     /locus_tag="J8I47_002719"
     CDS             complement(2221..2643)
                     /locus_tag="J8I47_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324117.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2782..3212)
                     /locus_tag="J8I47_002720"
                     /pseudo
     CDS             complement(2782..3212)
                     /locus_tag="J8I47_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1067 a          740 c          560 g         1111 t
ORIGIN      
        1 taaagttctg tcaaatatag tcactttata ttactttctc ttgagtaaaa aaacatgtga
       61 ggtcgtaaac aaagattgaa ctatctacat tcatactcta taatctgttt atttgcagac
      121 tgataatagc aaaagaaaac ttataatcaa ataatatcaa catttgatca cataatttga
      181 gattgaatgc tgatttacct atgttttacg taatggtctt tagttatttt tgctcataaa
      241 atgcataaaa cacgcgtttt tatgtgtgct tgaaatttct tggctctata atttctaaga
      301 ctgataaaaa tattctatcg gttctttttg caaataaaaa aacatataga agctccatta
      361 gattgaatca ccacaacaca atcaaaggag cattccgtat gcttgatttt cattctataa
      421 aactactttt aggaatcaaa gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga
      481 agtaccattg tttcatcgat catgagaaaa gcattcacct ctgtctgcag atgttcttat
      541 atcgctctgt cagtcctatt aataaagtac tgttttgttt tcaaaaaaac tattacgagt
      601 actttttaag agaatgcctt gcaaatattc atcacacacc gttatataag tcaaatagtt
      661 tctttcagtc gtcaagaaaa gattctcctg tccttggcat tatcagttaa gtagcaactt
      721 aattctcgga cttgttctac tctagcaaaa tttattttca tcaacttacg tccaaatgtt
      781 tttttgaata attacttcat cgcgggtctg gaagacttgc ctcattcgat aagtcactag
      841 cttttggcgt ccttcaggat ttctagtata caccaactga catttgggca gcgacgattg
      901 atttaattcg tttagaaaaa ggttagcgat ttgcttattt cggccaaagc gttctggtac
      961 cgtcagaact atctttttcc cttcgattaa ataccgcgga ttctccagcg gtgctaaaag
     1021 ctccctcata gcttcagtaa aaagctgtcc ttctcgatag ccagcattct caagatagca
     1081 tctaaattcc tgctgatcag ccgctacgat tacttttcta tcactaactt caccaaggcg
     1141 ctgcaacgtc gtttgaacgc atatggccaa gctttttttg cgggcacatt ccttttccca
     1201 tagcttttgt cgcctacgaa tttggaaata ttccagcaag ttacctttaa atatagctga
     1261 ccagaagctt agaggctcta tattttttac tactatcggc aatgaagttt cttgtttttg
     1321 ctttacctga tcgactttcc taacgacgat tggcatagat aggtgccagc catcagctaa
     1381 agcagtactc cattgttctt tcaaccgact tcgttcacaa gaaaaataga acatcgactg
     1441 agtataagtt tccatcgtgg tggtcgtata ttccagtggt aaagcaaagc gatcgccacc
     1501 actctcaatt tttggtgaag attcaaaggt cagacctgaa atattgccca aacgccgtag
     1561 cattcttttc aaatcaggct gttcagacag aggaactccc ggtaatccaa tcgctaaatg
     1621 ccaaatgttc gttactttat tttgtgcacc taggcgtaag ccccgccctc gtagttgttg
     1681 tgtttgcaca aaagtggaag cttgattagc caagatcaac agattaattg caggacaatc
     1741 ccagccttct cctagcaaac taacggttcc taccaaaata ttcaacttct tttcggttaa
     1801 taggcgagta actgcttgca tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa
     1861 ataacccgac atataaggat gttcattcat acccgtcgct agttctgcca gctcaacagt
     1921 tactagcatt ttcggtaaca gtaagaactc tccacagatg gcaccgattt gctgagtgtt
     1981 acctaataga cgaacaactt catcaaacaa aggaaccacg ccataagcta aggtaccatc
     2041 actgcctgct aatacctcct tgcaaatacg atccatcaaa atgaccgctg tcagttcttg
     2101 cttcagctga acttcttctt ttaacaaaat ttgacaaact gcttgctttt tctcaggaat
     2161 cgcctgacta ccaacagtgt ctggatactc tggaaacaaa tgcagcgact catcaaacaa
     2221 tcatccagct tttttcaatt gataaaaaat aggcaattcc ggttgatctt caaacatctg
     2281 ctgataaata gtgatcagct cttttttacc taaagatgga actgaagctt ttttaccttg
     2341 ctgcttagaa ctgcctaata gctgccaatg ttcgggtatc aattcatagt tatgatgatt
     2401 caagtaaatt aatgcactag tgaacagctc aaaattttca taaatttcct tcgaatgctc
     2461 aagcggatgc tgaacaaatt ctaaagaaag caaataatcc accgtctctt ggctttccat
     2521 taacgcttcc accagctcat tttgtttctc taaaaaggta aaataatgct gagtagtttc
     2581 ctgattagcc ggaatcaaat atagaaaatc ctgatgagga catagaacct cctcctttac
     2641 caactcggga atgctaattt cttcgtcaat caggccattt taactcaata tattgcttcc
     2701 actccgtctg agagctatca atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg
     2761 gttgtttttt cttcaagtca atcaacaatt caccccagct tttttttaaa tgatgagctt
     2821 catcaaaaaa ctaacagctc cagctgatca ccataagttg gctcctcctt cagtcggttg
     2881 taaatggtct gatacgtttc cactgtaata tgactaggat gttcaatcgc cccagaaaga
     2941 tagcaactat ctgctggatt tactaagaaa tattcctcga ttgttgttaa ccactgtttc
     3001 ttcaatatta gggaaggaac caaaattaag gttttacaat ccaaacgtcg gatcatctca
     3061 attcctaaaa aggtcttgcc agcccctggt ggagccacta gatggactga attgttattt
     3121 aaataagtag caaagttagt caatatacgc ttctgatagc tacgccactg aattttttca
     3181 tcagatagcc acatcccagg tccccctctc acgtattttc tctatcataa tctaaatgaa
     3241 tacagcaaaa aaaagatttc ttcaagaata tgtgttttta tagtaatatt ttacttactc
     3301 taaactcaaa tgaaaaggta aaaaaactgg tagtacaatc ttttaaattc tcattgtttt
     3361 gtacgttctt tactatcaaa aaaacaaaaa aaacgcgttt ctacgcgtga ccaaaatttt
     3421 tagctctata atttctaaga ctgatagaaa atcattctgt ttttgaagtc ttttttca
//
LOCUS       DADOZM010000197         3418 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.203, whole genome shotgun sequence.
ACCESSION   DADOZM010000197 DADOZM010000000
VERSION     DADOZM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.203"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            68..751
                     /locus_tag="J8I47_002721"
     CDS             68..751
                     /locus_tag="J8I47_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAZ6324118.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="J8I47_002722"
     CDS             753..1589
                     /locus_tag="J8I47_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ6324119.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="J8I47_002723"
     CDS             1600..3354
                     /locus_tag="J8I47_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6324120.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         1072 a          689 c          646 g         1011 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctc
//
LOCUS       DADOZM010000198         3257 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.204, whole genome shotgun sequence.
ACCESSION   DADOZM010000198 DADOZM010000000
VERSION     DADOZM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.204"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            106..414
                     /locus_tag="J8I47_002724"
     CDS             106..414
                     /locus_tag="J8I47_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAZ6324121.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="J8I47_002725"
     CDS             441..740
                     /locus_tag="J8I47_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAZ6324122.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..1465
                     /locus_tag="J8I47_002726"
     CDS             914..1465
                     /locus_tag="J8I47_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAZ6324123.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1611..1904
                     /locus_tag="J8I47_002727"
     CDS             1611..1904
                     /locus_tag="J8I47_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324124.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1897..2193
                     /locus_tag="J8I47_002728"
     CDS             1897..2193
                     /locus_tag="J8I47_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324125.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2264..>3257
                     /locus_tag="J8I47_002729"
     CDS             2264..>3257
                     /locus_tag="J8I47_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAZ6324126.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQ"
BASE COUNT         1225 a          456 c          650 g          926 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat tggcagatag
     1441 tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat aagtagtttt
     1501 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1561 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1621 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     1681 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     1741 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     1801 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     1861 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     1921 ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc gaatatagat
     1981 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2041 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2101 gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg ttgtcatgct
     2161 ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat taattgactg
     2221 ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa aatggttgtt
     2281 attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata cgaggactaa
     2341 agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc aatcaactga
     2401 acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat
     2461 tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat ctactcaact
     2521 agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa gctacaatcc
     2581 tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg atggaaattg
     2641 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2701 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2761 gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2821 tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac agttaaaaca
     2881 aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag aagaatctac
     2941 tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt caagttggga
     3001 agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt ctcctgataa
     3061 aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3121 tgaaaatgga agctactatt caattaatat tgtaacggat gataatggaa atacaacaat
     3181 ggctggtgga aaacaattcc gtgtttatac agatggaaga atttctcaac gtattggaat
     3241 gactaacaat tttcaaa
//
LOCUS       DADOZM010000199         3224 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.205, whole genome shotgun sequence.
ACCESSION   DADOZM010000199 DADOZM010000000
VERSION     DADOZM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.205"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(552..2165)
                     /locus_tag="J8I47_002730"
     CDS             complement(552..2165)
                     /locus_tag="J8I47_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAZ6324127.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="J8I47_002731"
     CDS             complement(2306..3214)
                     /locus_tag="J8I47_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324128.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          555 c          571 g         1060 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttct
//
LOCUS       DADOZM010000200         3051 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.207, whole genome shotgun sequence.
ACCESSION   DADOZM010000200 DADOZM010000000
VERSION     DADOZM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.207"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1199)
                     /locus_tag="J8I47_002732"
     CDS             complement(<1..1199)
                     /locus_tag="J8I47_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAZ6324129.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNT"
     gene            complement(1364..2845)
                     /locus_tag="J8I47_002733"
     CDS             complement(1364..2845)
                     /locus_tag="J8I47_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ6324130.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          911 a          489 c          555 g         1096 t
ORIGIN      
        1 tgggtattct tcgcttctat ttttccattg gtgtaaggat agatcatcgc gttggtgatg
       61 ccttcttcat aggtcagaag gttttggagc ttttcccgaa agccgtcatc taacgtttcg
      121 ggaagttctg ccaataagga gaaaaataag tcagggtctt tgtctcgaaa ggcttcaact
      181 aattcatgaa aatagggata cgcctccttc agggacgtag aaaagccaag caatcgatca
      241 atcatcatcg cttccgtaag aaatgggtat tttggtgctc gaaaactttt ccatgttttg
      301 tattcataat ggttgatgtt tgcacgattt tttagcaaaa agcgccagtt ctttttcagt
      361 ttttctgcct gacttttctg tcctacttta cgaagttcat tcatttcacg gatacgcaac
      421 tcattaaagg cttgattcat gtgtttgata atatgaaacc gatcaatcac tactttcgca
      481 tttggcagaa tacgtttggt aagctggaag taggcggcgt tcatgtctgt gactaaaaat
      541 tttacttctt caggattagc gcagcctaag aaatagcttg ttaatcgagg caatttacgc
      601 gtaggcaaga catcaattaa ttgtcctgtt tcgccatctg cgcaaataaa gctcatttta
      661 tcttctatgg aagcgtgcga acgaaattca tcaaccatca atactctggg gagaatcctc
      721 ttagattgct ttggtaagta gcttttaaac tctttcaacg tacgaataac ggtggtcaaa
      781 gatacctgac aatttttcgc aataaaagat aaagatactt tttcagtcag taaagaagca
      841 attttatatc tgacatgatt cgcgattgaa tgtctgggtt gaacaaaata actttgagcc
      901 gtccaatggg ttcgacagtt tttacaggta tagcgctgct tttttaagcg cataaccaaa
      961 ggcatgtgat tgtattgttc aaagcggaca atcgtttcct tcttcccatt tttcactaca
     1021 attactttcc cgtttccatc taccacagta gaaccacaat ttctacaggt atggggagca
     1081 ggtgagaaaa cagcatcgac gatcaacgtc tttttcttct gaaaggtctc gtaagagacc
     1141 tctgtaatca tcaaatcttt ctctattaat cgcagcattt ttttgataga atcattcata
     1201 tagcatatcg tcctctcagt tgtttatttt gtcgtgattt aatcatacta gagaacgata
     1261 tgttttttaa tacctaaaat aaaaatgggg ctgaagaatc aattctgatt catcagtccc
     1321 ataaattata gagccggaat aagaggatgt gacaagaatt ttgtcacatc ctctttgttt
     1381 tgttctcata accttttttc atatacagtt catgaaccgc ttttattaac ttgccaaata
     1441 acactgtatt gtgcgttgta tccagataga aagtctccgt ttcatctgaa attttctcat
     1501 gctgatctgt aatgatcaaa tcagcatgtt ctgaatatgc ggtaatttta acagcatctt
     1561 tgcgaaataa ctgctgcaaa tggctttgaa gatgataatt tcctgtaaac tgtttggtta
     1621 tctgcaaatg gatgagtaac tgctctttcg tttctgtttg tagtaagtgt tggatcaatt
     1681 gactaaggta ttgactgatt tggtttcttt ccccattttt tgaaaaaatt gtatcgattt
     1741 tttctcttaa tttcttttcc tgtataaaag aactagaatg attggacgtg gcttctggaa
     1801 agcaaagtgt agaaaaacta ctgctggatt cacccaacag ttcgaaaaaa acaactgcca
     1861 acacaagaaa ataaaaataa attggtattt tattttctaa atggttgaga ggaaactgtt
     1921 gagttaagta agtcatcaat tgaccgactt gtcgacaaat aaggtttttg cttgtggaaa
     1981 ataaaaaacc aatctcctgt ttttgtcgaa aagagatttg atttggaaaa aaacaataga
     2041 aaaaataatt aaagtaggct ctctctgttt ctgtctcttc acaagggcga ctcactgaaa
     2101 aagaagtaaa gtattctgaa taatcatgtg tatgctgcaa taagtccatc aattcagtga
     2161 tttcagaaga agtttttgga aaatatgcct gttgtttgtt tcgtaatatg aataaagtta
     2221 acagcaagta gatatttttt tcttgttttg aaattttttc tatttgctca tctgtactgt
     2281 tcatttttag tgcatttaat tcaagtgagg aaaatggcca atcgttacct ccaaatgtat
     2341 ctgaaagaaa aaagtaaaga tagagacgga tagataactc atttccttca agagtacagc
     2401 aattttttac agataggatc cgtaaatgga atttttttaa ggaatgattg agctgttttg
     2461 tcagtttttc gagataggag ggcgatatat aaagttgttc aagtaaatat ttttttggta
     2521 cagcagtttg tggataattg attagtaaca tcaaatagtt aaaacgattg gattctttta
     2581 ggtagcataa ttttaactta gaaaaaacag ctaacttttt agtttgatcg gtttctttgg
     2641 tttggatctc tcctttatga ttagaataaa taaattcatc aatcaataaa aattgcttga
     2701 gtgatacatt tatttttcgg atcagttctt tgattgtccg ttttggaata tgtaagtcat
     2761 tggataattc ttgtaaagaa caattatttt tttgaatgac catttgtaga acaagtaact
     2821 ctttttttct tgcttcaatt agcataaata cctccttttt attctgtttt tgtatatagt
     2881 tagacaaaaa ctgtgagtgg aaagtcattt cagtctgtgt tattctcaca gagtgtcgcc
     2941 ttctttttta tataacgtta tttaattttt ttgttacctt gtttattcta ttgtttattt
     3001 cctagggtat tttgtcaaga tattcttgtt tttaaggcgg aaaaattgat t
//
LOCUS       DADOZM010000201         3024 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.208, whole genome shotgun sequence.
ACCESSION   DADOZM010000201 DADOZM010000000
VERSION     DADOZM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.208"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1243
                     /locus_tag="J8I47_002734"
     CDS             <1..1243
                     /locus_tag="J8I47_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6324131.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="J8I47_002735"
     CDS             1256..1888
                     /locus_tag="J8I47_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ6324132.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="J8I47_002736"
     CDS             2093..2320
                     /locus_tag="J8I47_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAZ6324133.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="J8I47_002737"
     CDS             2389..2655
                     /locus_tag="J8I47_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ6324134.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="J8I47_002738"
     CDS             2645..2914
                     /locus_tag="J8I47_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ6324135.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DADOZM010000202         3016 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.209, whole genome shotgun sequence.
ACCESSION   DADOZM010000202 DADOZM010000000
VERSION     DADOZM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.209"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(106..1053)
                     /locus_tag="J8I47_002739"
     CDS             complement(106..1053)
                     /locus_tag="J8I47_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ6324136.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="J8I47_002740"
     CDS             1299..1763
                     /locus_tag="J8I47_002740"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAZ6324137.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="J8I47_002741"
     CDS             complement(1825..2868)
                     /locus_tag="J8I47_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAZ6324138.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          995 a          598 c          577 g          846 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttct
//
LOCUS       DADOZM010000203         3013 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.210, whole genome shotgun sequence.
ACCESSION   DADOZM010000203 DADOZM010000000
VERSION     DADOZM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.210"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            177..359
                     /locus_tag="J8I47_002742"
     CDS             177..359
                     /locus_tag="J8I47_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAZ6324139.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="J8I47_002743"
     CDS             462..746
                     /locus_tag="J8I47_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324140.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="J8I47_002744"
     CDS             762..998
                     /locus_tag="J8I47_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324141.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="J8I47_002745"
     CDS             1020..1274
                     /locus_tag="J8I47_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324142.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="J8I47_002746"
     CDS             1356..1541
                     /locus_tag="J8I47_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAZ6324143.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="J8I47_002747"
     CDS             1608..2003
                     /locus_tag="J8I47_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAZ6324144.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="J8I47_002748"
     CDS             1993..2370
                     /locus_tag="J8I47_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324145.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="J8I47_002749"
     CDS             2371..2766
                     /locus_tag="J8I47_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324146.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DADOZM010000204         2896 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.211, whole genome shotgun sequence.
ACCESSION   DADOZM010000204 DADOZM010000000
VERSION     DADOZM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.211"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            786..1172
                     /locus_tag="J8I47_002750"
     CDS             786..1172
                     /locus_tag="J8I47_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ6324147.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1266..1457
                     /locus_tag="J8I47_002751"
     CDS             1266..1457
                     /locus_tag="J8I47_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324148.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1971..2303
                     /locus_tag="J8I47_002752"
     CDS             1971..2303
                     /locus_tag="J8I47_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6324149.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2599..2754
                     /locus_tag="J8I47_002753"
     CDS             2599..2754
                     /locus_tag="J8I47_002753"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAZ6324150.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1059 a          471 c          450 g          916 t
ORIGIN      
        1 tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga
       61 gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc
      121 attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt
      181 acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt
      241 ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc
      301 aaagacaata attattatga tccgttatta tctaagcact atacagataa taggaatttt
      361 tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta
      421 ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata
      481 cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc
      541 tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc
      601 ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac
      661 atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg
      721 cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt
      781 gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg
      841 tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac
      901 aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga
      961 ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt
     1021 tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata
     1081 aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac
     1141 tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc
     1201 ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg gaagaaggta
     1261 gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat gaagaagctg
     1321 ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg agaactggaa
     1381 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
     1441 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
     1501 aatttcgaac aataaatttg aaactatctt ctttataatt ataaagtatc gattttacag
     1561 cgttctataa tgattcatag aatctaaaac gaatgatttt aattatcaac gttttttaat
     1621 tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta ctttatttac
     1681 gtatgaagtg ggcaggaggt cgaatccaga actgggctat ggtacttcaa cgaggacttc
     1741 tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat ttattcaact
     1801 atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt ggttgttaaa
     1861 aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa gttgggctat
     1921 gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta ttgagaaaaa
     1981 catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa aaagtaacta
     2041 ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga tgggtttcag
     2101 aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta cctgataaag
     2161 cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac gagtgttcta
     2221 cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg atgaaggaat
     2281 cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat gcacaagagg
     2341 atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa tggtaaaaaa
     2401 atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgat ggatattact
     2461 tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt agcaactcgt
     2521 attctacgtt actatatggt agattcacac atgaaaaaag agattgtcgt ccgtccatta
     2581 caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag ccagcatcgg
     2641 tcttttattt atcaagagct gttgaacgac caccacatca cacattcagt gagccaaact
     2701 gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa atgagaatta
     2761 atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca agattattta
     2821 gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc ttgacaattt
     2881 gaagcgaaga aaataa
//
LOCUS       DADOZM010000205         2800 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.212, whole genome shotgun sequence.
ACCESSION   DADOZM010000205 DADOZM010000000
VERSION     DADOZM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.212"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..171)
                     /locus_tag="J8I47_002754"
     CDS             complement(<1..171)
                     /locus_tag="J8I47_002754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324151.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPKRK
                     TPRKIAIWHGDRI"
     gene            complement(174..335)
                     /locus_tag="J8I47_002755"
                     /pseudo
     CDS             complement(174..335)
                     /locus_tag="J8I47_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(350..709)
                     /locus_tag="J8I47_002756"
     CDS             complement(350..709)
                     /locus_tag="J8I47_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAZ6324152.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            complement(706..1557)
                     /locus_tag="J8I47_002757"
     CDS             complement(706..1557)
                     /locus_tag="J8I47_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6324153.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1573..2403)
                     /locus_tag="J8I47_002758"
     CDS             complement(1573..2403)
                     /locus_tag="J8I47_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ6324154.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2406..>2800)
                     /locus_tag="J8I47_002759"
     CDS             complement(2406..>2800)
                     /locus_tag="J8I47_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324155.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
BASE COUNT          666 a          607 c          433 g         1094 t
ORIGIN      
        1 aattcgatca ccatgccaga ttgcaatttt tctcggcgtt ttccgtttag gcttttcagc
       61 ccacattgat ttaccgagct tttggacttc tgcaactatt tctttgtctt cttgccaatt
      121 ctcagaatgt gtcagttcga tgattcgttt cattgctgct ttcttatcca cgctcattcc
      181 tccaatcgat ggattttcct tcttaaattt tctatgtgca aatcgattgc cttcctcgcc
      241 gtttcattga ccatcactgc ctttgtccgt tacagatcgt caatctcacg ctgaaggctt
      301 cgaatacgca tttgaatcac ttcttctgtt gtcatgatgg atcacctcgt taaaaccgtt
      361 cttccttgaa cgtattccga tatttcttcg ctaagatcag tggtactcga tattgactgc
      421 agaagagttt cgctttgatt ttgaaatctt ttgtctgcat ccctttaaca tctacgactt
      481 tgactagttt gccgttttta taaaatgtga agtcgggaat atactcgatc ttgcgatact
      541 tctttccgtc tagttcaaat ttcggcatca gctcaaatct ttcctgaagt tttactttcc
      601 agccgttcgc ttcagcttgc cacaaggcta gatcgtaata ctctgcttct gcgatagaat
      661 caaacttgat acctcgatga acagtttttt tattacggta tttattcatg cgatactacc
      721 tttcactggt tttatgcgct tgtctgctgt ttgttggaat ttcagcgcat aaccttctga
      781 attcttaaat atcctagaaa caattctttc accgtaggct tctcttagtt caggaccaga
      841 taagtttgtt gtgatgatcg ttgccttgtt ctgtctggct tctaagagcg tgtttaacgt
      901 gttgtttgta aattgcctac tatttgatac cccgctacct aattcagctc caatatcgtc
      961 aaaaaccacc aaatcagttg ttttgatatc ggctataagc gatccttcaa tttcttttct
     1021 cagttcagca ttgttataag aaaactttat ttgctctaat aactcttgat agcttataaa
     1081 aagtattttc ttgtcataat ttgagcgctc aagtatttcc caagctgtcg ccattgataa
     1141 gtggcttttt ccgcttcctg atttccctga tagaatgaaa tgtgcaggat ggttcagtag
     1201 gacatcattt acatagcttt tagctctttc taaagcaatt ttcgtttctt ggtccactac
     1261 gtgataattc tccattttgc atttaaacaa agttttatct gttaataccg aaccattttg
     1321 aaaaaaactc aatgctcgtg cttttaagct gtcgttatat atccgttcgg tctgtatatc
     1381 ctctttcaca cgtaacgctt tataaccaca actcatacat gttggtttac aacgttctga
     1441 accatcctta tttttagctc gccaactata caaaggttcg ctacattctg gacattttcc
     1501 gctttgcact aatactcttc ttattagctt ctccatagca tttgctaggc tttccatgtg
     1561 atgcatctcc ttttaaattg gcaagtcgtc atattcacta ggattgctgt actgtagttt
     1621 ttgactttgc tttttatgat tcttcttgtc tgctttgatt tcgaatttga gcttctcaaa
     1681 tttttctctc aatttcttag cacttctaat atttccaaac caaaattcat ttgtaggtag
     1741 ccaattgatc acatactcaa tcgcttctat agatgcttta tctctttctt ccatcaacct
     1801 gattgtgtct gcccattttt cgatatctac tttgttcatt tcttttggaa aatcttcagt
     1861 taaattcctt tgcatttttt tagcaaggcg taagtgttcg tcagaatact tacctttctt
     1921 ttcttcttta tctatatctt tatcttcttc tatatcttta tctgtaccgt cacgtgacgt
     1981 cacgctaacg tcattttcca atttgagacg ttcctgtctc tttctttccc tgtatttacg
     2041 gtttctttca gcatttttta gccttacttt atccataccc tcgatatttt gatgtttttc
     2101 ccaattactg atggcaatta gtccatcact gcttagatca atcatgttga aatttgccaa
     2161 tgtagttagc gctaagcgaa ccgtatttac gtttttgcca aacaatgtag caagcatttc
     2221 ttcggtataa ggcatgttcc tctggatata tatcagacca tcgtcgttag tctttcctgc
     2281 taaaactagt aatcgaatcc atataacgat gatggcatcc gactcaggaa cagcttggat
     2341 taaccgtatt ttttcatcgt caaacatagt agttttaagt ttgatccaac ttatctcagc
     2401 caaatttatc ctcctatcct taacttttta atcgtttcat gacttaactt gattcctttg
     2461 atttgatatt tatttttaaa attgatcaca cctattttgt gcttctccgt gtgatggatt
     2521 ctgcagagtg ctgcaaatgt gtactctgaa tgatcaactt cttttcgctt tcgtcttcct
     2581 agcgctttgt caaagtgatc gatgtcagct cctgttttgc cacagatgca acaaactctt
     2641 tttgtaatgc atttgtagaa gtaatattct tgattcgctg gtaaaatctc atagccttct
     2701 ttgaaaggaa tatgatgttc aaagatgaaa tctaagatga tatttgctaa gacattagca
     2761 tcactcacag tcgtattcga ttcatctttg aggcttattt
//
LOCUS       DADOZM010000206         2756 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.213, whole genome shotgun sequence.
ACCESSION   DADOZM010000206 DADOZM010000000
VERSION     DADOZM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.213"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(491..1243)
                     /locus_tag="J8I47_002760"
     CDS             complement(491..1243)
                     /locus_tag="J8I47_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6324156.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1258..2505)
                     /locus_tag="J8I47_002761"
     CDS             complement(1258..2505)
                     /locus_tag="J8I47_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ6324157.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(2581..2727)
                     /locus_tag="J8I47_002762"
     CDS             complement(2581..2727)
                     /locus_tag="J8I47_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAZ6324158.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          954 a          460 c          338 g         1004 t
ORIGIN      
        1 gcagcgctgt agttggtgct gcaggaggac ctatttcggc cgttacagga ggtattggta
       61 atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag ttaaatttat aaaaacagga
      121 aagtaattat ctcaaatcct atcaagtaca atcataggat tgcacttgac aggacaaatt
      181 ttttaactaa aaaatgaaaa acactttcta aaaattagta gaatttgcca aatctttcgc
      241 atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa aacaagaaaa
      301 gaactattca tttggccctt ttttggttcc agaccataga aatattcctc cagtaactaa
      361 tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact ttaataggat
      421 tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa tctccctaaa
      481 gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt gctttcccta
      541 aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta aaatctattt
      601 ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac ataggatatt
      661 tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta gtacacaatg
      721 ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata tcaaaagtaa
      781 atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt gttgcatcta
      841 tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca tctagtgttt
      901 gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg ggtaacatct
      961 catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg attacttctg
     1021 ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat attccttgct
     1081 ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgtgtt ttcaatacta
     1141 tcttaaatac tttatcgtga aaaagaatga aattatcaat aattcgttct aactgattta
     1201 attgtataat ttgatcttca cataatatta ttggataact cataaatcct ccttccctca
     1261 aaacaacaaa ataacttgag tagtaaattg atacggatcg ttttgatagt tcacaaacat
     1321 attgggattc tttttattga tttcctctat actagataaa cctaaccctt gatgtccctt
     1381 ttttgttgtt attccttttt tcattagagt atctatagag agtagtatct ctctacaact
     1441 attcgatatc gaaatttcta tctgtttatc atctcggtaa atagctagag acagttctct
     1501 atcttgactc tctatagatg cttcaattgc attatcgaga ataattccaa gcaccctaat
     1561 acagtcaaat attggaacag gaacttcaat aatagtactt ggacattcga atttacaaca
     1621 aatcttattg ttatttgctt ggtatagctt cgctatcaac aaacttttca aatattcatt
     1681 ttttatattt tttaaataac gatattcaaa cttattggtt agataagaat tagaatagat
     1741 ctctaattct tttatttctt tttccaatat cgaatcatgg ttttgattag cgatttcttt
     1801 taaactcagc aataaatttt tatagtcatg tctaaatctg gcaaattttt cttgatcttt
     1861 ttccagtgaa tcagtatatt tttttagata aaccaattct tttcttttca attgctgttc
     1921 atattgttct cgttgcctaa tagttattct aataaataaa aatagaacaa caaacatttg
     1981 aatgattaaa aatattaaga caccaaacac aaaatgatca aatacttgat attgatgagc
     2041 aacataagaa acaaataaac caattgtaag taaataaatc atgaatatag cagtaagttt
     2101 agaactggac tgttcaacaa tcctatttat agctaatttt ctatataacc aaataaacat
     2161 cagcaaaaac aaagaatcaa gcattatctg gaatactaca taaccatacc cttttatccc
     2221 ttcaaatgaa aacatatacg tcataaccat ggaagtaata atagatctga aatcatcaat
     2281 aagcatacat aatagtaatt tatttaaaag cactatatta gttttcttct tttcctttaa
     2341 tataaaataa cttccgatta taggtatagc tgcggaaaca tcagtaaata attcaacaaa
     2401 aaaagtgagt ggtatcaata aacatacaaa aagatatttt cttttgttaa gatcagtatt
     2461 agtcatataa aataagagaa aaaagaaaaa gtttcccacg aacattctta atagataagc
     2521 ttttgtatcg atagtaatca agagatcccc tcctgatttt aggatatcat aaaaaaaatt
     2581 ttagttacac tttttaaata atgaaaaaac acattccact aaatttgatg ccggttttcc
     2641 ttgaggtttt gttccagcac ctcctttaat ttttttaagc tcttgatctg aacattgttt
     2701 agcatttagt ctattttttt cttccattac gattccccct acaaactata aatact
//
LOCUS       DADOZM010000207         2740 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.214, whole genome shotgun sequence.
ACCESSION   DADOZM010000207 DADOZM010000000
VERSION     DADOZM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.214"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            933..1607
                     /locus_tag="J8I47_002763"
     CDS             933..1607
                     /locus_tag="J8I47_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ6324159.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1604..1945
                     /locus_tag="J8I47_002764"
     CDS             1604..1945
                     /locus_tag="J8I47_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324160.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1975..2334
                     /locus_tag="J8I47_002765"
     CDS             1975..2334
                     /locus_tag="J8I47_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAZ6324161.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2538..2717
                     /locus_tag="J8I47_002766"
                     /pseudo
     CDS             2538..2717
                     /locus_tag="J8I47_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT          930 a          457 c          588 g          765 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag tgccttgacc tgagtattag agaaacaaaa cggaaaaata
       61 gcttctcata ttttaccgtt tttttagcta atatcgaggg tcagctgatt gaacaccgtt
      121 tatatcaaga ggttgtgcgc ctgccgttca gctccgagtc acaaggagta atttcaaaaa
      181 acagtttttc atgtttgatg actattgtga cttgtgaccg aggagttggc cggagagcaa
      241 cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct ttttttaaaa tacgggataa
      301 aggaagtttc cagcagacaa gagaagaaag caaaagaaat tgctatttga ttcacaaggt
      361 tctataacga aatcgcaatc aatcaaacat atacaaagaa ggttattcaa atggaaatct
      421 gatttgatga tgcttctgct gttgaaataa agcagcatct agtacctgaa aacaaactaa
      481 tagtaacatt cgaagatgga gtaggtccgt attttcagca tgcaatgatc catatgtaag
      541 tttaattcat ccttgaaatc atcggaccag ataaagttat gaccaatcta tcccaagcaa
      601 tctagaagag atattagtaa aagaacattt actggaagtc ttagatgagc ctataaatat
      661 atgttctgat ggcgtcatca gtggtctgat tgacggaagc ttaggtgtga tcgatttctc
      721 tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa agtataaaaa
      781 actagacact tgatgagcgt tcattcaatt gattgaaggg actaggctgg tgtcattttt
      841 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
      901 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
      961 acagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
     1021 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatgaaattt gaatgcatga
     1081 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt
     1141 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
     1201 ctagcgatca ggctatattc ggaactgaga aatctgcgca gcaatttgaa tccgtttatc
     1261 gaaaattact aaatgaactt caaacagcgg aaatctgcaa catcatcttg atggagccgt
     1321 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
     1381 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat
     1441 tcaatgcttt agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc
     1501 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
     1561 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
     1621 ggcagtaaaa gaagatttac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
     1681 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
     1741 aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg
     1801 ggtggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
     1861 ggaagaaatc caaaaaaaat ttgcagaata cttgatgctg gaagctaaaa aaagaagaat
     1921 aaaatcaatt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
     1981 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
     2041 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
     2101 aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
     2161 atgtcattga tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
     2221 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
     2281 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaaagag
     2341 aataataaag gttagaaaac gcatagagtc aggtgggata catgtatttc acctgactct
     2401 atgtgttttc ctgtataagt tctcccatgt gacttttacg tcttttattt atatgggatt
     2461 actgtttatg gaaagttgaa ataattttca ttaaagaact ctttctatat ttttgaaagc
     2521 gtagtggagc ttcgaatatc tgggaaggac attttgcagg gattgaaaaa agatattgtc
     2581 ctatcgaaaa attaatgaat ttagacaaat tgccagcaac aggcttcaaa cttagttgct
     2641 atcctgtcaa gatcaagaag gcaagtgcgg gttggataag agcaggggca atgatcgaag
     2701 agaaaaagaa agaataagcc aaaacatctg tatggaagaa
//
LOCUS       DADOZM010000208         2567 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.215, whole genome shotgun sequence.
ACCESSION   DADOZM010000208 DADOZM010000000
VERSION     DADOZM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.215"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..81)
                     /locus_tag="J8I47_002767"
     CDS             complement(<1..81)
                     /locus_tag="J8I47_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAZ6324162.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLS"
     gene            complement(403..624)
                     /locus_tag="J8I47_002768"
     CDS             complement(403..624)
                     /locus_tag="J8I47_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324163.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(722..967)
                     /locus_tag="J8I47_002769"
     CDS             complement(722..967)
                     /locus_tag="J8I47_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324164.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1207..1821
                     /locus_tag="J8I47_002770"
                     /pseudo
     CDS             1207..1821
                     /locus_tag="J8I47_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2191..>2567)
                     /locus_tag="J8I47_002771"
     CDS             complement(2191..>2567)
                     /locus_tag="J8I47_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAZ6324165.1"
                     /translation="IEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSY
                     LSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIM
                     EKYKAPIQADPFSNLDESDLPF"
BASE COUNT          861 a          448 c          354 g          904 t
ORIGIN      
        1 gcttagattg taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg
       61 tttgccttta aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta
      121 aaatagattt tttggaaaac tttgcaacag aaccgttttt actatatact tcttcccaga
      181 acttgtccgt ctccttcaaa ttatctctac ctctcgagtt cattgggtgg tttgcccatt
      241 tattaaacat ggaatcacct ttctcatact catatattta tgtcctattt caattatatt
      301 tatatatgct taaataaatt tgtatcctga aatttattgt aatatgaagc tgtattttac
      361 atatagtaac caaagagtta ataaaataaa aagagcgaac cattatagat cagctctttt
      421 ttcattgctg tttttaatct tatctagaat cacttcaaat gtttcaaagg tatttacatc
      481 taactgacat aaccaaagca agtacttctt ttctttgtaa gtccaatcaa tattacggta
      541 agaggacatt agattatggt accttgctct ttgttcaact gaagccaatt cccagcgttt
      601 ttcaaatatt ttttcttctt ccattattga tccctcgcaa gacgctacct caaacttaaa
      661 taatgggcag cgaaagtcta attccttaga tctaagtata gcaaacaaat atgaaaaaaa
      721 cttagaaggt agtaaaagtt cttccgtctt ttgctttgct tctattctta atgtgtttcg
      781 ttatcggtgc cctacaaaga ttttcattaa ttacacactc atttattgtt gtgtattttc
      841 tgacatttct ctcttgatca attattttaa ttccatgggc atcctgatag ttcataggag
      901 aggagcggct atcagaaata gttgcaccta tctcttgaac ttcttttaca acttctggaa
      961 aatccatcca tcctgcttca gctgtcaaaa gtaataatct ataatattta tctttccttt
     1021 tcaatctaag aaatcgtcac cccattcttt ttcttctatt ataaactatt taacttttag
     1081 aattcaaaaa ccgtttgtct atttagagtt taccctaaat agacaaaaat ataacattga
     1141 aaaatttaga gttctaacgt atgattaggg tgtattataa acgttataga aaagtaggga
     1201 ataaatttga aaattgctta tattagagtt tcatccatcg atcaaaatga acaacgtcaa
     1261 attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta tcgaaaaaca atctggagcg
     1321 acgattagtc atcggccgat tttccaggaa gctttagatt ttgtcagaga gcaagatatt
     1381 tttattgtag aagccattga tcgacttgga cgcaattatg acgagattat cgattcagtg
     1441 aattatctaa agaagaagaa tgtgcgactt attatcacca gtttaccgat catggctgaa
     1501 gcaattggaa atcctctcct agataaattc atcaaagact taatcattta aatctcagcc
     1561 atgattgctg aacaagaacg tactgaatcg aaacgacgcc aggcacaagg aattaaaatc
     1621 gctaaagcaa acggcgtcta taaaggtcgt cctaagttat acagtgcaga caccaaagat
     1681 cctcaacgtc gccttgtcta taggagtatc gttcaggatc ttgagaatgg ggttgctatc
     1741 tcaaagattg caacagacta caatgtgact aggcaaacaa tttaccgaat aaaaaaagaa
     1801 atcgaccaac taattgtatg atttcttttt tgaatcacgt aattattaat tagctttgtc
     1861 atcaatacag acaatcattt tagattcatc tagctaagtt gattacattt atgttaaaaa
     1921 gaatttagtt aacataaata taattgatgc ataaatcgtc cttatttaaa ggcatttaac
     1981 attgacaact tagtataatg tatcttatgt aaactaataa gatttgaatt ttattttata
     2041 cacactcgat aatagatcaa ttttatggtc ccccgcattt acgattgcac aagagaaaca
     2101 taaagtcgac ctattagcat ttcttttcaa ttcaaacatt aaaaaagttt atacctctgc
     2161 tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt aaatctgatt catccaaatt
     2221 ggaaaatgga tcagcctgta ttggagcttt gtatttctcc ataataacag atatcttttt
     2281 ttcatttttt aaaaaatacc ttttaaattt cttatagtcc tctgaaatca aaatatagcg
     2341 tgatggatac tcatcaaaag aagaatcgta ttcttccttt aaaattcccg atttacttaa
     2401 tttttctagt acatcaaata ctctatcact caagtaactc cttaaattgt tatcactttc
     2461 ccattgaact acttttctat aagttgaatt atcagttgaa aagatagaat tttcatccat
     2521 aaacgcatat attagcagca atctttcatc attgtcaaat tctattg
//
LOCUS       DADOZM010000209         2510 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.216, whole genome shotgun sequence.
ACCESSION   DADOZM010000209 DADOZM010000000
VERSION     DADOZM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.216"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            9..359
                     /locus_tag="J8I47_002772"
     CDS             9..359
                     /locus_tag="J8I47_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6324166.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            773..1591
                     /locus_tag="J8I47_002773"
     CDS             773..1591
                     /locus_tag="J8I47_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ6324167.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1664..2023)
                     /locus_tag="J8I47_002774"
     CDS             complement(1664..2023)
                     /locus_tag="J8I47_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ6324168.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          843 a          362 c          447 g          858 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtttta taaagcaatc attcctgaga
       61 acttagatga ggttaaactt tatttatggg aagataacgg agaaattgtc ggttttagtg
      121 gtgtaagtgc agaagaatta gttatgctat ttcttgatcc agattttatt ggtaagcgat
      181 atggtagtca aatattaatt gagctgattg aaactgaaga tataaaaaga attgatgtta
      241 atagtcaaaa taaatatgca aaacattttt atttaagcca tggatttgaa attgaatctg
      301 aagaagactt agatggtttt ggtaaaccat accctataac gcatttagta aaaaaataat
      361 tcatgaatgg tttattacct gattgaacat gaataaaagt aatattttta ttttgatggc
      421 ctatttctag gttatgaatt atccagatca aatttgtgta ttacattcag taataaggtg
      481 cttatcttag gatcaactta tgatactagc tgaccaagga tgtaccagtt atagaaaatc
      541 agttaaagta aaaatgataa aaatcttatg tcaacactga tttaggctga cctgtgggtt
      601 aggagaaaga caggtcggta gcttttaata caaaaaatgg cttgtatgta tctagatagt
      661 taactgaaat tagaaatgtt cttattttta acggagtaag aaaaataggc tgctttaggt
      721 ctgaatactt tttcaatata atgaagatat caaaggcgac aggagtgttt ttatgaataa
      781 gaatcctcat aatctaaagt tggtagcagt agatatggat ggaacatttg ctcgtactga
      841 ttacacatac gatatcccaa ggttcaaggc tgttttatcg cgaatgaaag ctgttgattg
      901 tcaatttgtt gttgcaagtg gaaatcaata ctaccaattg agatcgttat ttccaaatta
      961 ttatgatgaa ttaagttttg ttgctgaaaa tggtgctttt gttaaagata aaacagaggt
     1021 actattttca gccgatatgc ctagaaaagc gatcgaggtg gtccttgatt tatgtaaaga
     1081 ctatcctgaa gtaaaaaatg tgatgtgtgg tctgaatagt gcctactgtg aaagaggaaa
     1141 ggtgagtcaa gcattttttg atctaaccaa tatctattac catagattag aatgggtagc
     1201 tgattttgca gaagttcaag ataaaatttt aaaatttgct ccgactatgc ctgttgaaaa
     1261 aaccgaattt tacgttgaat tgttccgtga aaaattagcc gggctattag tacctaccag
     1321 tagtggtcat ggttctgtag acttgattct ttctgagtgc cataaaactt tcggcttgaa
     1381 aatgttagtt gagcgcttgg ggattaatcc tgaccagtgc gttgcttttg gagatggggg
     1441 caatgacatt gagatgttgg agtattgtgg tctgagttat gaaatggata atgcaacgga
     1501 agctgttaag caagttgcta agcatcaatg tccttcaaat gacgaagacg gtgttttagt
     1561 gacgctcgat cgattgttcc ctaacacgta aacactaggt aattcatcaa aaaacctcga
     1621 tataagaaat attcgaggtt ttaaaactat atttagaaca gatttacgaa gcgtctgatg
     1681 agtcaaaata gacttggaca ttgctatcta aaataccact ttcattcttt aaacgaattg
     1741 tttgatttga tggagttacc tctaatacta aatgcccatt aataaaggta gtatcgtatt
     1801 cagatgtttt aactgttgct tcttcgcaag gtattgttaa atcatatgct ggaatataat
     1861 gttgtgtgga aactaataaa aaatgatcac caatcataca gcatccctct gctgaagaaa
     1921 attgattaga gccatcattt aaagttaaca agaatccatc ctttaaacga ggtacttgct
     1981 taaatacttc tggttgtact tctaaaaata aattatcgct cattttcacg accaccatcg
     2041 acttccacag ttgaaattga cattttattt aacacaccta ttccccctta actataactc
     2101 aattctatat aaaaaatatt ctattctcaa ataattgaac ttttcaaatc gccaatttac
     2161 ttcggcttta tcagagctca ttcatatcaa gagcgattaa cgctgtaaaa attaaaccga
     2221 agatctgata aattttctct attttctaca atatttaaaa cgcttcctta gtctattata
     2281 tatctatttc tcttaggagt atgtttagat gtttgaataa tagttgccca actttaaagg
     2341 ttattcttat aatattttac ttcattttcc aatagaaaat tcaaaggatt actggttttt
     2401 ctaaaatagt ttagtttaaa tacataagta tcctccgaca ctgtattagt tcataatcat
     2461 ctaaatacaa aattttcaaa taaaaatatt tcaagctcta tcgttgacga
//
LOCUS       DADOZM010000210         2454 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.217, whole genome shotgun sequence.
ACCESSION   DADOZM010000210 DADOZM010000000
VERSION     DADOZM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.217"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            132..344
                     /locus_tag="J8I47_002775"
     CDS             132..344
                     /locus_tag="J8I47_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324169.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="J8I47_002776"
     CDS             506..1024
                     /locus_tag="J8I47_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAZ6324170.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="J8I47_002777"
     CDS             1031..1543
                     /locus_tag="J8I47_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324171.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="J8I47_002778"
     CDS             1814..2437
                     /locus_tag="J8I47_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6324172.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DADOZM010000211         2437 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.218, whole genome shotgun sequence.
ACCESSION   DADOZM010000211 DADOZM010000000
VERSION     DADOZM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.218"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            101..868
                     /locus_tag="J8I47_002779"
     CDS             101..868
                     /locus_tag="J8I47_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ6324173.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="J8I47_002780"
     CDS             1060..1458
                     /locus_tag="J8I47_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAZ6324174.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            1678..2082
                     /locus_tag="J8I47_002781"
     CDS             1678..2082
                     /locus_tag="J8I47_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAZ6324175.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2099..>2437
                     /locus_tag="J8I47_002782"
     CDS             2099..>2437
                     /locus_tag="J8I47_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324176.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          894 a          344 c          445 g          754 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgtcccatg ttacaatcag tctatacaag aaatgagttg atataacatg
     1681 gaattagata caaataatca ttcagtattt cttctttatt accaccttgt tttagtaacg
     1741 aaatatcgta gacaagtgat tgatgatgaa atatctgatt atgccaaaac tacttttgaa
     1801 agaatttcag aatcgtatca cattacttta gtggagtgga atcatgataa agaccacgtt
     1861 catattatgt tcaaagctca acctaaaaca gaattgacaa aattcatcaa tgcctataaa
     1921 agtgccagct caagattgat aaaacgtgac tttccaagag tcaaacaatt tctttggaaa
     1981 gaaatgttct ggtctaaaag cttttgtctt ctgacaactg gtggcgcacc aattgatgtg
     2041 attaagaagt atatccaaaa tcaaggaaac aatcataaat agaaagtagg tgaactttat
     2101 ggaacaacta aaagcataca aattcagaat ctatccaaca gaggaacaag aaatcttctt
     2161 tgccaaatct tttggttgtg tccgtaaggt ctacaatcta atgcttgatg accgaaaaaa
     2221 ggcgtatgaa gaagttaaaa atgattcttc taaaaaaatg acttttccga caccagctaa
     2281 atacaagaaa gaatttccgt ttttgaaaga aattgatagc cttgctttag ccaatgccca
     2341 acttaattta gataaagcat acaagaattt ttttcgggat aaatccgttg gatttccacg
     2401 tttcaaaagt aagaaaaatc ctgttcagag ttataca
//
LOCUS       DADOZM010000212         2407 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.219, whole genome shotgun sequence.
ACCESSION   DADOZM010000212 DADOZM010000000
VERSION     DADOZM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.219"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            5..1150
                     /locus_tag="J8I47_002783"
     CDS             5..1150
                     /locus_tag="J8I47_002783"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAZ6324177.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="J8I47_002784"
     CDS             1321..1779
                     /locus_tag="J8I47_002784"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAZ6324178.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="J8I47_002785"
     CDS             1938..>2407
                     /locus_tag="J8I47_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAZ6324179.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DADOZM010000213         2400 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.220, whole genome shotgun sequence.
ACCESSION   DADOZM010000213 DADOZM010000000
VERSION     DADOZM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.220"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            207..2069
                     /locus_tag="J8I47_002786"
     CDS             207..2069
                     /locus_tag="J8I47_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6324180.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="J8I47_002787"
     CDS             2069..>2400
                     /locus_tag="J8I47_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6324181.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DADOZM010000214         2340 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.221, whole genome shotgun sequence.
ACCESSION   DADOZM010000214 DADOZM010000000
VERSION     DADOZM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.221"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            113..2278
                     /gene="acm"
                     /locus_tag="J8I47_002788"
     CDS             113..2278
                     /gene="acm"
                     /locus_tag="J8I47_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAZ6324182.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          922 a          353 c          455 g          610 t
ORIGIN      
        1 tcaatttttt agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg
       61 agagatgata tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa
      121 gtgtagaaat attattttat caatgttatt tattattact aatataacat cactgattcc
      181 tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt
      241 ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa
      301 aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat
      361 tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac
      421 tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga
      481 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      541 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      601 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      661 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      721 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      781 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      841 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      901 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
      961 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
     1021 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
     1081 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
     1141 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
     1201 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
     1261 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
     1321 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
     1381 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1441 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1501 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1561 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1621 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1681 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1741 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1801 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1861 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1921 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     1981 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     2041 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     2101 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     2161 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     2221 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     2281 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
//
LOCUS       DADOZM010000215         2260 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.222, whole genome shotgun sequence.
ACCESSION   DADOZM010000215 DADOZM010000000
VERSION     DADOZM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.222"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..572
                     /locus_tag="J8I47_002789"
     CDS             <1..572
                     /locus_tag="J8I47_002789"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ6324183.1"
                     /translation="CQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTARE
                     VEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIE
                     GFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(928..1500)
                     /locus_tag="J8I47_002790"
     CDS             complement(928..1500)
                     /locus_tag="J8I47_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6324184.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1516..2121)
                     /locus_tag="J8I47_002791"
     CDS             complement(1516..2121)
                     /locus_tag="J8I47_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAZ6324185.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          709 a          460 c          341 g          750 t
ORIGIN      
        1 tttgtcaaaa aaactgttgc cattccatca tttcaaaaga gaatctctta aatcaggact
       61 tttctactga aacgatctgt gagaaatggg cagctgacat tacgtacatt cctactaaga
      121 aaaatggttg gtgttactta tcttcaatca tggatttaca cacgaaaaga attattagtt
      181 atacattttc aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa
      241 tacattatca cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag
      301 caagagaagt ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag
      361 gaacacctta tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag
      421 tctacacgac tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg
      481 aaggatttta taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat
      541 ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct actaaagaca atcaacaccg
      601 tagggatatg gagataaaat caatagagtc aatagattat ttatcgataa taacactaaa
      661 aaagccacgt tcctaaacgt gacttacgct gaacgatatg tgttgtttgg tagacgaaca
      721 aatcgttcat tataaatata tgatggtatc ctaatatagt aagcgtggct agcatacctt
      781 taattacttt accttttaca gttaccatac acattttttg agcagaaatc aagtaaataa
      841 aaaaatatta tatttcacaa catgctaata gactcatatc tagaacgcat taagtattta
      901 cctgtctggt tcgctgagac ttctttttta actttcttca attttcttct ttattctaaa
      961 aagcgtactg cgactgaaac cggttatagc ttcaacttcc ttatacgatt tacctgaagt
     1021 aagcagttca tatgcgtgcc tcttcttcgg tgtgattgtt gctttcggac gtccttctct
     1081 gaaattagga ttattctttc ttgcaaacat ttttccttct tgggtacgtt ccacaatcat
     1141 atcacgttcc atttctgcta cactcaacaa cgtccgtgtc accattcgcc ccatggacgt
     1201 attttcaatc gttcctagat tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc
     1261 aatgatctct aatgcttctc tcgtgttacg agcaaatcga tcaagcttcg taacaactat
     1321 ttcatctcca ggcttcagac cagccaacag ctgattgaat tttggccgat cccgttttgt
     1381 acccgtaaat ttttctgaaa aaatattctt cttctctacg cagttctcaa gcaactgttg
     1441 aatttgttct tttgtacttt gagaagcggt tgagacacgt gcatatccat atttcgtcat
     1501 ttggtccacc tacttttaca aatcatctat cgtatactca gaataccctt catagaaata
     1561 gctgacatcg attcctttca tatataactc gcgatcgtca attttatcag tcagagcatt
     1621 ggaaattaga tagcgtattt ccaaatcgtt tatgggactt cgctccattg cagaaagata
     1681 gtctgcttta tcgattaagt tccaatctac aactttttgc aattcttcct ttaaaatgag
     1741 atccagccaa attctagtac ttcggccatt tccttctcga aatgggtgag cgatattcat
     1801 ctccacatat ttcttgataa tctcttcaaa agaagtttgt ggcatctgat caatatgctg
     1861 taacgaatgt tctaaataca taactggtgc aaatcgaaaa tttccttttg caatattgac
     1921 cgatcggatt tttccggcaa aatcgtaaat atccgaaaat agaaattcat gaatttcaga
     1981 taaaccttta aatgtaccaa cttcgatttc atttattttt ccacaatcgt acaattcttt
     2041 cgctttttgt ttactgagtt tttcttcaac cttagccaac tcaacttgat tggttaatcc
     2101 caatttattc tctaaaatca tgcgaaatct ccttaacttt atgacataag tttatgaaac
     2161 actctcaacc ccattatatc gcgattttgt ttatgtgtca aaaactatta agtttctgac
     2221 ccccgataga tttttcaagt gtctctattt tgcacagtct
//
LOCUS       DADOZM010000216         2258 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.223, whole genome shotgun sequence.
ACCESSION   DADOZM010000216 DADOZM010000000
VERSION     DADOZM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.223"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(574..939)
                     /locus_tag="J8I47_002792"
     CDS             complement(574..939)
                     /locus_tag="J8I47_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324186.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1015..1260)
                     /locus_tag="J8I47_002793"
     CDS             complement(1015..1260)
                     /locus_tag="J8I47_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324187.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1330..1584)
                     /locus_tag="J8I47_002794"
     CDS             complement(1330..1584)
                     /locus_tag="J8I47_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324188.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1748..2071)
                     /locus_tag="J8I47_002795"
     CDS             complement(1748..2071)
                     /locus_tag="J8I47_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324189.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          854 a          371 c          390 g          643 t
ORIGIN      
        1 ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa gcgttattat tttagtctat
       61 tttcaggggg ggaaccttat gataaaaaaa ctatttttac tatcaatatt aacgctagca
      121 atggcaggtt gttctgactc tggcttagca actgaactga tcacaaaatc aactgaagcc
      181 acgacagagg agttaaaatg tggaaacgat cacagtgact acagaggctt aaatcgacga
      241 tgcatgggaa tattaggcag aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt
      301 agaagaagca acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg ctgtgcatcg
      361 tatacagacg tcgaagatgc ggtactttta aaccacgtca ggagatgttc atttatgtct
      421 agggaagttg gcgatgaacc aaaactagag tacgctttta gttttgcacg tgaggatgaa
      481 gataacccta aaacttacga cattttagct tgatctaaga tggttaaagc tttttaatga
      541 agtaagagga gatagaaggc ttttctagtt atattaggta agataaattc ctttttgctc
      601 caaatttgct aaacattctg ctagataaac ttcatcgtgt tccggataaa tcggcacgtt
      661 gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat acataggatt
      721 tttccactct ggtgctgggc tgtaaccacg tttttccatt tcatccatga caagctgatg
      781 atattggaat aatctataag gagaatagtg aaaaacataa tccacagtag catgtttctt
      841 tccccagcca tttcctctta atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg
      901 aggaagcttt gaaatcaatt tttcatgcca taatcgcata gaaatgtctc ctttatatat
      961 agattgttaa ccttagacgt taaatgaaaa aaccagcacc aaggctggaa aaaatcactt
     1021 cagtttgctt gctgtttctg agcgaccaat taggtaatca acggaaatat cgtaaaagtc
     1081 agcgatatag attaattcaa tgatgcttgg aatttcttct ccttcttcgt acttacgata
     1141 tttgaataaa ggaatactta acgcgctaga aacagctact tgagataaag atcggccagt
     1201 tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa atagcttctt cgatactcat
     1261 ttaatcacct ttcattatag tagaatagtt ataatgacta tagaaagcta cacatgcaat
     1321 caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga
     1381 caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt
     1441 aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc
     1501 tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa
     1561 caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat
     1621 aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa
     1681 tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt
     1741 gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa
     1801 agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat
     1861 atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca
     1921 tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg
     1981 aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg
     2041 caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt
     2101 ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata
     2161 agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta
     2221 tagttgaaaa gtaacaaaaa taaattttat ttatgatg
//
LOCUS       DADOZM010000217         2223 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.224, whole genome shotgun sequence.
ACCESSION   DADOZM010000217 DADOZM010000000
VERSION     DADOZM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.224"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(104..1531)
                     /locus_tag="J8I47_002796"
     CDS             complement(104..1531)
                     /locus_tag="J8I47_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAZ6324190.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1524..>2223)
                     /locus_tag="J8I47_002797"
     CDS             complement(1524..>2223)
                     /locus_tag="J8I47_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAZ6324191.1"
                     /translation="KYVLLSGADYPIKDNEYIYNYFKKNSSVEFIRGIDLDQIKHKEF
                     YYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWW
                     ALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPL
                     ELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLN
                     RIDKEILKVGNIDE"
BASE COUNT          839 a          353 c          256 g          775 t
ORIGIN      
        1 ataaataaat aaatctcctt ttgattctcg gtaaaaattt tgataaacat ttaatatatt
       61 aatcaaatgt aaaggagtcc aagcaatgaa agctgtcttc atattaattc tcctttttta
      121 aatacaagta agaaatgctt cccaaataat accagaaaac aattgaaatg acatcgcgct
      181 tattgaaaag tacatgagcc tccaccattt catctataaa aatagatagt ataaaaatta
      241 aaacaatacc aagaaacgca taattcactt tacttctaaa aaatgttttg agtaaactaa
      301 agatatttaa cacatagaaa gcatagatac atataaaagc aattatacca cttgatacaa
      361 gtacttgaag aaatccatta tgcatgtttc catgtgctct ttttagttca ttcacatctg
      421 tagtatcaag atctactttt ttcaaaaatt ttgcatcgct attcccatag aaatcggtct
      481 ctccataacc aaataacaaa gttggtctta taactttaag cgcagctttc caaatcgtaa
      541 aacgaccagc tgaaatttcg gagttatcac tagaatgctg taatattact tctttttctg
      601 gaagtggagt tttatctgat tcatttgact ctataacttc tacttttcta tcaatcgata
      661 caggaattgt tgctaaaaaa tccttagcat aattagtacc aaaagaaaaa tataccaata
      721 gaactaataa tatgcttacc atccatttga tttttacagg tagtacttct ctaatgctct
      781 ttctaaattt tgaattggca aacaacaaaa gaaataatac taaaaaagaa caaattacta
      841 cctgggcacc tctagacgaa gaaagtgtaa tataaaatat ttgtacgata aaattcatca
      901 tataaaaaac acgaatcgtt tttgttattt ttgaaattaa tacactaatt aaacttaaaa
      961 taatagaagt atatccaaac atagctccaa catttggact tgtatagaca ccaaataacc
     1021 gatgttcaat aaaaccttgt cttgcaagta atcctggcgt tcctgttggg acatgataag
     1081 aaatcaaaaa aataaattga tagatagata ttagcgcagc taaaaaaatt atgataatga
     1141 aaacattatt aaaatacacc atttttttct ttttttcctc ttcactttgt gaaaagtctg
     1201 caggaaaaat tactatcaaa gtaatcagca aatatccaaa attataaata gatggaatta
     1261 aaaaattttc tctgttaatg attatagtta agataaaact aatcattgct ccaatcagta
     1321 gccaagaaaa acgcatttta aaaaaatttt tctttataaa aacctcatac aaaactataa
     1381 ttccacccca agcaaaagct attttcatcg ggatattagt tacattaaaa gtactaggca
     1441 taaaataaag cagacaaatg attaaattta ttactcgaaa cgcatctcga cttaataaac
     1501 tcttctctaa aacaggtact attctattca tctatatttc caactttcaa aatttcttta
     1561 tcaattctat ttaatagttt atcagagtaa tccgatttaa cttttcttac aaataatttt
     1621 tcactttctt ttatttgatc tatatccctt tcatcaaacc atttgttcat ggaaggatca
     1681 ataatatgaa tatttgctaa tttcgaagtt tgtaaagtag tttcttcaat atttttcaat
     1741 tcaagtggta aatctggtcc tccaataaca tttttatttt tgaacgagga attaaaaaaa
     1801 agtgtatgaa aaaatttttc atctggggca aacataccta ttttaaaatt caggtagtct
     1861 tgttgatttt gttcatacat ttgtattaat tctgttaggc attctttcga caaagcccac
     1921 cattgagaac catgatacaa atcaaattta tggtgtctaa tttttggggg aagtttaatc
     1981 atcctcagac aacgattaat tatagctcta aaaattttaa atgcagttgt gttattccta
     2041 ttaattctag gataatcatg tttttgatat acatctatat gtttataata aaattcttta
     2101 tgtttaattt gatctagatc tattccacga ataaactcca ctgaactatt ctttttaaaa
     2161 taattataaa tatactcatt atctttgatt ggataatcag caccagataa taatacatat
     2221 ttt
//
LOCUS       DADOZM010000218         2209 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.225, whole genome shotgun sequence.
ACCESSION   DADOZM010000218 DADOZM010000000
VERSION     DADOZM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.225"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(205..1104)
                     /locus_tag="J8I47_002798"
     CDS             complement(205..1104)
                     /locus_tag="J8I47_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAZ6324192.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITWLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="J8I47_002799"
     CDS             complement(1114..1470)
                     /locus_tag="J8I47_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ6324193.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="J8I47_002800"
     CDS             1825..2121
                     /locus_tag="J8I47_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAZ6324194.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          690 a          421 c          376 g          722 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccaa gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgattt
//
LOCUS       DADOZM010000219         2205 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.226, whole genome shotgun sequence.
ACCESSION   DADOZM010000219 DADOZM010000000
VERSION     DADOZM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.226"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..607
                     /locus_tag="J8I47_002801"
     CDS             <1..607
                     /locus_tag="J8I47_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ6324195.1"
                     /translation="LPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVV
                     DKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLE
                     AGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQ
                     SSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            741..2084
                     /locus_tag="J8I47_002802"
     CDS             741..2084
                     /locus_tag="J8I47_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ6324196.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          877 a          270 c          394 g          664 t
ORIGIN      
        1 tctaccaaaa ggaactactt atacttggaa agaaaaagta gatgttagta cagctggaaa
       61 caaaaaaggt actgtagtag taacatatcc agatggatct aaagaagaag tagaagttac
      121 tatttctgta gtagataaaa aagcaccgaa taaacctcaa gttgatccta ttacagaggg
      181 tgaccaaatt gttactggta aaactgaacc aaatgcagag gtgacagtta cattaccaga
      241 cggaagtcaa caccatggga cagctgataa aaatggtaat tttacagtta aagttcctaa
      301 attagaggca gggacaaaag tgatagtaac tgcaactgat gaatctggta atactagtga
      361 acctactaat gtagttgttt cttccaacaa aaaagacagt ggaaaaaacg gtagtaaagg
      421 tagtaagact gacaatcaag gtagcaacag caatcaagac aaaaatagag gcaaaagcca
      481 gtcctcaaaa gtttttccta agacgggaga gagtgacagt aatatcttta ctattagcgg
      541 tggtttaata ctgttaggaa cgttaggatt attagggtat aaaaatcgaa aaaaagagaa
      601 tgaatagaaa atcaatttct taagtcttct ctgagaagag gaagaatctt tttcttcctc
      661 tttttgcttt tttatgatgg gaaataagtg ttagttaaag atgaatatac tttttataga
      721 aaggaaaaga atgtaattaa gtggaaaaaa tagtaattat tggttcaaac cattcagggc
      781 tagccgcagc tcgatttcta aacttaagcg attcaaatta tgaagttatt ctaatcgata
      841 aaaaatcaga aattagttac ttggaatgtg ggacatcgtt atttataaaa ggagaagtaa
      901 gtaatttaga agatcttttt tatcaaaata gaatgacact aaattctgag ataaaaaagt
      961 tatttctaga gacagaagtt gacaaaattg atattcaaaa taaaaagatt agttgtgtaa
     1021 aaaaagatgg tacaagattc tctcaagaat atgacaaagt tatattagct acaggttcaa
     1081 agcaaattat gctagatata ccggggaata atttagctaa tattttttcg ataaagaatt
     1141 ttccaaatgc aaaagagatt tttaaagctt tggatgatag tactattaaa aaagtaggaa
     1201 ttattggagc aggatatatt ggagttgaaa ttgctgaagc cataagaaaa agagggaaag
     1261 aagtatatct tttcgatgtt gcagatagag tgttgtcgac ttattacgac cgtagttttt
     1321 cagataaagt tgaagaaata ctatcaaaaa atggaataca tctttgtcta tcagaaagtc
     1381 ctaaaaaata tttaggaaga aaaaagattg aaaaaatagt cactacaagt ggtacccaat
     1441 actctatgga tatggtcgta caagctatag gatttctatc taatagtcca attggggaaa
     1501 aggaactctt gagagatgtt agtggagcat atctaactaa tgaataccag caaacaaata
     1561 taaaagatat atatgctatt ggggattgtg ctacaacttt ttttacgtcc attaatagaa
     1621 gtagtgttga tttttctatt tctaatgctc taagaacagc ttatattgca actcaacata
     1681 ttaacaatca agattttaca ccttctggtt cccaatttac aaatggcttt tctatattta
     1741 attacaattt ttacgcagca ggtttgaatt taaaaacagc acgattatta agtgagtcta
     1801 tagattatat agaaatagaa gatttcatta gacctgcttt tttgagaaaa aatagtcgtg
     1861 taaaattaag aattagcttt gaaaaaaata caaagagaat agtaggtgta caactttgta
     1921 gtcaagaaga tttatctgga ctattatcaa tgttttctct tgctatagaa gaacaaatta
     1981 cattagacaa attgaaaatg ttagattatt tatttcatcc tagttttagc caaccctata
     2041 attttatttt aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa
     2101 tcaagaaata tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa
     2161 aacactatta aagagaaaaa agagaggtaa atcaaatgaa aaaat
//
LOCUS       DADOZM010000220         2204 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.227, whole genome shotgun sequence.
ACCESSION   DADOZM010000220 DADOZM010000000
VERSION     DADOZM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.227"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(857..1147)
                     /locus_tag="J8I47_002803"
     CDS             complement(857..1147)
                     /locus_tag="J8I47_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324197.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1355..2047)
                     /locus_tag="J8I47_002804"
     CDS             complement(1355..2047)
                     /locus_tag="J8I47_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAZ6324198.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          616 a          417 c          319 g          852 t
ORIGIN      
        1 tcatctaatc tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta
       61 aagagtaaaa agatacccca aatccctatt agtaacaaga tcagttcttt gttattcgtt
      121 atttttctat tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa
      181 catagcttta agcattactt aaagataact taaaagttat cttttaaata cttaattgtt
      241 aaatacctta tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat
      301 ccagaaaaat ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat
      361 aaactgccaa agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg
      421 tttgtctcaa aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa
      481 ggcgaggctt cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc
      541 ggtactctcc aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta
      601 gtctcttttt atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga
      661 tctgttcaat ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac
      721 aaatacttaa atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt
      781 gggttcaaat cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt
      841 ctcagccgct tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata
      901 gacttttctc atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa
      961 caaattgctt tctattatta taataacctg taacgactaa cttcatccct tcgtctactt
     1021 cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac
     1081 ggatcaacgg tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt
     1141 tctccataac cttcacctca ataccattat acgaacatac attccctttg taaagaagaa
     1201 actctgtttt atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt
     1261 cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct
     1321 tctttactaa tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa
     1381 tttgctgaga tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa
     1441 tatattgttt caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg
     1501 ttaactgagt ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac
     1561 catttacagt attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc
     1621 aatctgcatt cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat
     1681 tgatttcatt ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt
     1741 tttgtttttc tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt
     1801 attcttggac atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg
     1861 taagatctgt aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct
     1921 cattgattgt atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg
     1981 tgcctgtgta gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct
     2041 ttttcatttg catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa
     2101 acacaatgtc aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac
     2161 tcatttaatc ctcctttttt atctatttaa aaaatctttt ttat
//
LOCUS       DADOZM010000221         2190 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.228, whole genome shotgun sequence.
ACCESSION   DADOZM010000221 DADOZM010000000
VERSION     DADOZM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.228"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            361..975
                     /locus_tag="J8I47_002805"
     CDS             361..975
                     /locus_tag="J8I47_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6324199.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1140..1964)
                     /locus_tag="J8I47_002806"
     CDS             complement(1140..1964)
                     /locus_tag="J8I47_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324200.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          745 a          363 c          310 g          772 t
ORIGIN      
        1 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
       61 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
      121 cattgtctat cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga
      181 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
      241 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
      301 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
      361 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
      421 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
      481 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
      541 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
      601 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
      661 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
      721 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
      781 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
      841 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
      901 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
      961 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     1021 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     1081 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     1141 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     1201 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     1261 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     1321 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     1381 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     1441 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     1501 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     1561 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     1621 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     1681 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     1741 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     1801 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     1861 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     1921 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     1981 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     2041 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     2101 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     2161 ataaaaatta cttaactgta tccaaattta
//
LOCUS       DADOZM010000222         2167 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.229, whole genome shotgun sequence.
ACCESSION   DADOZM010000222 DADOZM010000000
VERSION     DADOZM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.229"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..914)
                     /locus_tag="J8I47_002807"
     CDS             complement(<1..914)
                     /locus_tag="J8I47_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAZ6324201.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="J8I47_002808"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="J8I47_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAZ6324202.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          734 a          381 c          417 g          635 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 atttttt
//
LOCUS       DADOZM010000223         2151 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.230, whole genome shotgun sequence.
ACCESSION   DADOZM010000223 DADOZM010000000
VERSION     DADOZM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.230"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..930
                     /locus_tag="J8I47_002809"
     CDS             <1..930
                     /locus_tag="J8I47_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324203.1"
                     /translation="KELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDE
                     IFIQGENYFTKILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKN
                     SIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIF
                     FDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEV
                     LLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNI
                     VLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            917..>2151
                     /locus_tag="J8I47_002810"
     CDS             917..>2151
                     /locus_tag="J8I47_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAZ6324204.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKIT"
BASE COUNT          733 a          272 c          333 g          813 t
ORIGIN      
        1 aaagaattag ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc
       61 tttaaacgta aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc
      121 gtttatgatg aaatttttat acagggggaa aattatttca caaaaatact tttctccaat
      181 gtttacaaaa aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat
      241 ttgaagaaga gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat
      301 aaaaatagca tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta
      361 ctttatgatg taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg
      421 aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt
      481 tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa
      541 gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg
      601 acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta
      661 cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat ttctttttca
      721 ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat
      781 gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt
      841 gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta
      901 agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc
      961 cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca taactattcc
     1021 tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt
     1081 tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag
     1141 ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact
     1201 ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt
     1261 tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat
     1321 tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc
     1381 ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt
     1441 tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt
     1501 agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag
     1561 atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc
     1621 acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc
     1681 ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg
     1741 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
     1801 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
     1861 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
     1921 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
     1981 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
     2041 aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac
     2101 gtatttttta ctttttattt ttcattataa tatcgctaaa aaaattacga a
//
LOCUS       DADOZM010000224         2102 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.231, whole genome shotgun sequence.
ACCESSION   DADOZM010000224 DADOZM010000000
VERSION     DADOZM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.231"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..424)
                     /locus_tag="J8I47_002811"
     CDS             complement(<1..424)
                     /locus_tag="J8I47_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAZ6324205.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="J8I47_002812"
     CDS             complement(533..1564)
                     /locus_tag="J8I47_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ6324206.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          655 a          337 c          360 g          750 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 tt
//
LOCUS       DADOZM010000225         2102 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.232, whole genome shotgun sequence.
ACCESSION   DADOZM010000225 DADOZM010000000
VERSION     DADOZM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.232"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            169..1539
                     /locus_tag="J8I47_002813"
     CDS             169..1539
                     /locus_tag="J8I47_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAZ6324207.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="J8I47_002814"
     CDS             1560..1844
                     /locus_tag="J8I47_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324208.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DADOZM010000226         2058 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.233, whole genome shotgun sequence.
ACCESSION   DADOZM010000226 DADOZM010000000
VERSION     DADOZM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.233"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..337)
                     /locus_tag="J8I47_002815"
     CDS             complement(<1..337)
                     /locus_tag="J8I47_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ6324209.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="J8I47_002816"
     CDS             complement(404..559)
                     /locus_tag="J8I47_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ6324210.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="J8I47_002817"
     CDS             1176..1397
                     /locus_tag="J8I47_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324211.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="J8I47_002818"
     CDS             1398..1655
                     /locus_tag="J8I47_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324212.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="J8I47_002819"
     CDS             complement(1791..>2058)
                     /locus_tag="J8I47_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ6324213.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DADOZM010000227         2034 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.234, whole genome shotgun sequence.
ACCESSION   DADOZM010000227 DADOZM010000000
VERSION     DADOZM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.234"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="J8I47_002820"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="J8I47_002820"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAZ6324214.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="J8I47_002821"
     CDS             complement(1431..1823)
                     /locus_tag="J8I47_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ6324215.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DADOZM010000228         2003 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.235, whole genome shotgun sequence.
ACCESSION   DADOZM010000228 DADOZM010000000
VERSION     DADOZM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.235"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..378
                     /locus_tag="J8I47_002822"
     CDS             <1..378
                     /locus_tag="J8I47_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAZ6324216.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="J8I47_002823"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="J8I47_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAZ6324217.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="J8I47_002824"
     CDS             1859..>2003
                     /locus_tag="J8I47_002824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ6324218.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DADOZM010000229         1982 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.236, whole genome shotgun sequence.
ACCESSION   DADOZM010000229 DADOZM010000000
VERSION     DADOZM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.236"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="J8I47_002825"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="J8I47_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAZ6324219.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="J8I47_002826"
     CDS             852..1040
                     /locus_tag="J8I47_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAZ6324220.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="J8I47_002827"
     CDS             1053..1610
                     /locus_tag="J8I47_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ6324221.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="J8I47_002828"
     CDS             1642..1884
                     /locus_tag="J8I47_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ6324222.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          305 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 tt
//
LOCUS       DADOZM010000230         1909 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.237, whole genome shotgun sequence.
ACCESSION   DADOZM010000230 DADOZM010000000
VERSION     DADOZM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.237"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            61..453
                     /locus_tag="J8I47_002829"
     CDS             61..453
                     /locus_tag="J8I47_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324223.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(626..808)
                     /locus_tag="J8I47_002830"
                     /pseudo
     CDS             complement(626..808)
                     /locus_tag="J8I47_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            795..992
                     /locus_tag="J8I47_002831"
     CDS             795..992
                     /locus_tag="J8I47_002831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324224.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1035..1610)
                     /locus_tag="J8I47_002832"
     CDS             complement(1035..1610)
                     /locus_tag="J8I47_002832"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAZ6324225.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1765..1909)
                     /locus_tag="J8I47_002833"
                     /pseudo
     CDS             complement(1765..1909)
                     /locus_tag="J8I47_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          634 a          313 c          272 g          690 t
ORIGIN      
        1 acgtaaatca attatttttc attttacttt aactaataat gtttatttgg agggattatt
       61 atgttagatg atagattgac gtgttctaat tgtggtacat tattttacta tgaggaagtt
      121 agaaatattg taaaacatag aaaaaaagaa atgcctatac gttgtccaaa ttgcaatgaa
      181 gttattaagc gaaagttgtc tcatggttat tttgtcagct acgaactata tgaagaaagt
      241 aaaataaatc tgaaaaaaaa accaaaatac tataaaaatt tgaaaactgg agctattata
      301 actgaaaaag aatttaaaaa tttagtgact gaagaagaaa gcaggtttta taatttgtct
      361 ggtaatattt gccctggatt tatggatcag attattgaaa atgagtcaat tgaatcaatt
      421 gaaaactatg ttcctgttga cttaccaaaa tagaaaaaat aatcctgttt ttacagtgaa
      481 ttattttaat tttgattttc aataaaaata tattacaata ataatcgtaa tagctaatga
      541 aacaatttct caatcttttt accactccaa acagggagcg tcaataattt tgtgtaaata
      601 actcgtcctt ctgcaaaata atgggttact cagtaaacat tgaagctaat gtatccgtca
      661 cttgttggaa gcctttatgg cttctattta agaatttttg attgtatgta tcaaagatgg
      721 atactaggaa acgctctagt gattcttcat tttgaaactg ctcttttctg cggctgtatt
      781 tcttgatttg tttattgaaa gattcgattg tagaaccaga aggaatggag aacacttttg
      841 aactttacaa caaaactact gttgataaat taattccaat tttagttcca actgatttaa
      901 atggtcatga tgttttactt cgttttaacg gatttttagg tgaggattat agatacctaa
      961 aaatttctga tcctcaacct ttaacagaat aataaaaaac gtgtaatcaa ttatttgatt
     1021 acacgttttt tatttcaatc ttcgttgtag tagtggttgc gacataccga aaggtaatct
     1081 tcattacctc ctactacaat ctgttcacct gaataaactg gaataccatc aacaatacga
     1141 gcattcatta cagccttttt attgcaaaaa tggcaaatag ttttaatttc ctcaatttta
     1201 tcagcaaatg tcaataatgc ttcggatccc ttgaagagat tattttgaaa atcattcttc
     1261 aatccatagc aaattactgg tacttcctca ttcattacga taagtgatag atcaataata
     1321 ttctctctac ttaaaaattg agactcatcg accaaaatac aatcaacttt tccgtactga
     1381 tcaataatct ccactaattc tccgtacaca tccatttcag aagtaattgc aatagctggt
     1441 ttcgaaacac ctatacgact ctcaactttt ccaaatcctg atcgagtatc tattgcgctt
     1501 gtcattatga taactctctt cccttgttct tcataattat gagccacttt cagtagctca
     1561 atactctttc cactattcat tgttccatat ctgtaaaata attgtgccat taaatttatt
     1621 cccttctttt acaattcaat tattccttaa ttccatctgc ttttatgcag ctgctcgttt
     1681 attccttgat atgtctataa catctactat ccctaaaacc aatttataag ccattagaga
     1741 tggtattcct gaacatctct catattgttt tttgagtggt atatcatctg aaaaactgaa
     1801 ggaatcatga aaatagcgac atacttatca tttagagttc taactttaaa tgcatttact
     1861 tcttcagtca gcactttagt catgttggac atggtttggg gcgtgtagt
//
LOCUS       DADOZM010000231         1881 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.238, whole genome shotgun sequence.
ACCESSION   DADOZM010000231 DADOZM010000000
VERSION     DADOZM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.238"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            153..1313
                     /locus_tag="J8I47_002834"
     CDS             153..1313
                     /locus_tag="J8I47_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ6324226.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="J8I47_002835"
     CDS             1329..1772
                     /locus_tag="J8I47_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ6324227.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          653 a          299 c          376 g          553 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaaac
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatcttt g
//
LOCUS       DADOZM010000232         1829 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.239, whole genome shotgun sequence.
ACCESSION   DADOZM010000232 DADOZM010000000
VERSION     DADOZM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.239"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            73..1101
                     /locus_tag="J8I47_002836"
     CDS             73..1101
                     /locus_tag="J8I47_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAZ6324228.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1157..1375
                     /locus_tag="J8I47_002837"
     CDS             1157..1375
                     /locus_tag="J8I47_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324229.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1446..>1829
                     /locus_tag="J8I47_002838"
     CDS             1446..>1829
                     /locus_tag="J8I47_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324230.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYN"
BASE COUNT          705 a          202 c          336 g          586 t
ORIGIN      
        1 atgatcacat tctagataat tttttggatt caaatgatga aatattatat ttaaataagg
       61 agtatgataa taatgaatag caaaaaactt cagatgggtg aaattgttag aggtcctagt
      121 caaatatcta ttgatgttac atataaatgt aattttagat gtaaacattg ttacaatgcg
      181 agtggtggag acaattgttt tatggataat gagttgactg atgatgaact gttagaactt
      241 acaaaagata tatgtaattt aaagccatat aatatttgtt tctgtggagg agaaacttta
      301 ctgagaaaaa aaatagtttt atcttgtgcc actttattaa aagaggcagg tattcctaat
      361 attgctattg ttactaatgg atcattagta actcaggatg ttgcaaaaga attattcaaa
      421 gcaggtatta atcgatttca agtaagttta gatggaaaaa atgctagtag ccatgaagca
      481 cttagattat atccaaacag ttttgaaaag gcaattaatg ctatcaaatt atttagagaa
      541 ttgtccgaag aagtggatgt tgctttttgt cctactagtt ttaattacaa tcaattacga
      601 gaagtatatc aactagtaaa tactcttaaa gtgaagtctt taagaattca accatttatg
      661 cttagtggta ggggaatgca aaatagggaa atagtgccaa ctgtttctca atatagaaaa
      721 ctagtttctg atattaacta tattaaaaga aatgaagatt ataccaagat agattgggga
      781 gatccaattg atcatatcat ccgacacaga actttattga aagatttttc gaattttgtt
      841 tctattaggt cagacggagg attaatggca tcaccttact tgccaattca agttggaaat
      901 attaaatgtc acagtctttc taaatattgg cgagcaggac tttataaagt tttttcttta
      961 gatttagttc aagatatagc tactcaagtt tcttcaatac aagatatgag taaagaatta
     1021 aaaaaaggac atattaaatg gcttaatgaa gatatagaaa ttgatattct cgatgaatac
     1081 aaacatgata ttttaattta ggtttatcat tggaaagttt agataaacaa agtactttta
     1141 gaaaggaggg aaaaaaatga tttctgaacc tcaaatgaat aatatgtatg aaagcccaac
     1201 tataatggga gctggtgaag ctgcgccaag agcttgttca gcaattatgg tgtgtgtgtt
     1261 attagcatct gcaggagtag gtatccaagc aggagcggtg tatacttatg ctgtagttgc
     1321 tacagtagct gtagctgcag ccgcagtttg gaaaacagta ggattagttg gttaggaatt
     1381 aataggagac taactgtctc ctattgattc caatgattaa gctaaggagg aattttaaga
     1441 aaataatgaa atacaataat ttaaaaccga aattacttaa tgagaacata gaatatataa
     1501 gagaagaaaa ttatggatat ttaaccatag ttcccaaaaa tggaactgaa ttgaaagata
     1561 acgttttcaa tccaacagta agatatatat tggctaactg tactggaaaa aacagtgtaa
     1621 aaaaaatagc gaatggttta aaaaagaagt ttcctagttt agaatatgaa aaattctatc
     1681 aagatgttgt aggaactatt aaatttttca aacactataa tattattgat ataggagatt
     1741 atagtatgga agaaaagaaa atatataaaa ttgaacaaaa tttagaatta gaattactaa
     1801 atgaatcaca ttatgaagaa atttataat
//
LOCUS       DADOZM010000233         1796 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.240, whole genome shotgun sequence.
ACCESSION   DADOZM010000233 DADOZM010000000
VERSION     DADOZM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.240"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="J8I47_002839"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="J8I47_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAZ6324231.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DADOZM010000234         1752 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.241, whole genome shotgun sequence.
ACCESSION   DADOZM010000234 DADOZM010000000
VERSION     DADOZM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.241"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            949..1422
                     /locus_tag="J8I47_002840"
     CDS             949..1422
                     /locus_tag="J8I47_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAZ6324232.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="J8I47_002841"
     CDS             1431..1658
                     /locus_tag="J8I47_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324233.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          588 a          272 c          260 g          632 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt ta
//
LOCUS       DADOZM010000235         1750 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.242, whole genome shotgun sequence.
ACCESSION   DADOZM010000235 DADOZM010000000
VERSION     DADOZM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.242"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            333..1502
                     /locus_tag="J8I47_002842"
     CDS             333..1502
                     /locus_tag="J8I47_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6324234.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          641 a          241 c          272 g          596 t
ORIGIN      
        1 attaacatac tactatattt taggtttcat attatataaa ttaataatat actaccattt
       61 gttggtagta taatttaaag gtaataagtt attcttttat tactatgttt gggttttata
      121 gcaaagaaag tttaataaga gtttggacct agcaaacaat attaatgagg tcattaaatc
      181 ttattatctt tagtgatata acgaaatatc tacacagaca aaatgatagc gtaaaatatt
      241 ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta
      301 ccacaaccga aattttatag gaggactttc tcatgcccaa ttttactaca gaaattatgg
      361 aaacactaat caataaaggt gatttagatg aattatttcg atgtcattta gaattagctg
      421 ttaattcatt attgcaggct gaattaacag cttttcttga ttatgaaaaa tatgatcgtg
      481 ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc acgttcattc agaacagagt
      541 atggagaatt aaatttggta attcctagag atagaaatgg taagttttct caacaaactt
      601 tacctgctta taaaagaacc aatgactctt tagaaaccac tattattcag ctatttcaaa
      661 aaggaattac tatgtctgaa atctctgaat tgattgaaaa aatgtacggg catcactata
      721 cgccacaaac catttcaaat atgactaaaa tagtttctga agatattatt gcctttaaag
      781 aaaggtcctt agaatccaga tactctgtca tttttatgga tgctactcac attcctttaa
      841 aaagacaaac tgtttcaaaa gaagcagttt atattgtaat tggcattcga ttagatggaa
      901 ccaaagaggt tcttgggttt agtcttgctc caactgaatc tgcttatgtt tggaaagaga
      961 ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt attagtcgta actgatggtt
     1021 taagtggcat taacgatagt attcatagtg tttatccaaa tgctcaattt caacaatgtt
     1081 gtgtccatat atctagaaat atcgctcata aggttcgtgt tagtgatcga caagaaatct
     1141 gtagcgattt caaattagtt tatcaagcaa gttcaaaaga agaagcaaat aatcaaataa
     1201 ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc
     1261 ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta
     1321 atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc
     1381 ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt
     1441 ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa tcaatgtttt
     1501 aacttgatca acagaatggg cttttccatt tacacaaaat tattgacgct cccgacaaaa
     1561 ttatgatctt gcgtaaagtt ataaaatatt aataaaaata ttttattaat gaaaagcttg
     1621 gtagcacggt tattcatatg ctaatttatt tcactttata gtaaggggag ttcttagtat
     1681 attgttcaaa cttaaatttc atataattta attatgtaaa ttagctattc taagaacttc
     1741 tacggataac
//
LOCUS       DADOZM010000236         1749 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.243, whole genome shotgun sequence.
ACCESSION   DADOZM010000236 DADOZM010000000
VERSION     DADOZM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.243"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..424
                     /locus_tag="J8I47_002843"
     CDS             <1..424
                     /locus_tag="J8I47_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324235.1"
                     /translation="SVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKD
                     NGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIE
                     GNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            421..>1749
                     /locus_tag="J8I47_002844"
     CDS             421..>1749
                     /locus_tag="J8I47_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAZ6324236.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMII"
BASE COUNT          610 a          204 c          318 g          617 t
ORIGIN      
        1 gtctgtttat gattttgagg gcggagaacc tgtcggagaa tatgaattag tatgcaaaat
       61 agcagactta gtaggaaaag ataatctaat tgttaagacg catcctagag atgtaagaac
      121 aatttataaa gataatggat ttatcgagga taaaaattca tcaatacctt gggaggcgat
      181 tcaattgtca ggcgatttta acgataaaat atttatgaca attaattcag gaagtgttat
      241 ttcaggtagt acaatgtctg caaaacctgt acgcacgtat tatatgtata aattgtgtaa
      301 catagaagga aatccgtctt gcaaaaagaa tgcgtatgat attgagcgat tgcttaagca
      361 aaaaacgatg aaaaacattt taaagaatgt gaaaatagca gaaagcttag atgatatact
      421 atgagcaaaa ctaaatattt aattaaaaat atgggaatat taacaataag taatttttct
      481 tcaaaaattt tagttttttt gttagtccct ttatatacaa gtgtattatc cacaacagaa
      541 tatggcacat atgatttgac aatttcaaca gccacattat tgtttccaat tttaacctta
      601 aacattgtag atgctgtgat gcgcttttta atggataaag ataaaaataa aaaaaatata
      661 gcatctatag cgattaaagt tatattttta agcgtgatag tctttggatc cgtaatgtat
      721 ttgttaaata ttacaaaatt atggcaagat gtttatggat tagaaaaata tatttttttg
      781 tattatatat cctatgtatg gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa
      841 gttaaagcta tgggcatagc aggtattatc agcacaattg taacaataat tgccaatatt
      901 ttcttcttgc ttattctgaa atggggatta attggttttt tcattgcaaa tattttatca
      961 cagattattt cagctactta tctgtcagta ttagtgagaa tatgggaata tatcgatttt
     1021 gaggctaaag atcctcagct aaagagagaa atgctaaatt actcgattcc attaattgca
     1081 actgttgttg gatggtggat taatagtaca gctgataggt atgtggtttc ctttatagta
     1141 ggtgtctcgg cgaatggctt attgtctgtg gcatataaaa tacctcaaat aattaatact
     1201 ttgcagggaa tatttataca ggcctggcaa atttctgcaa ttaaagaata tggagataat
     1261 gagactgcaa aattttatgg aaatacattt gttatgatta atttgttaat gtgcataacc
     1321 tgttcaagtc ttatcatatt aactagaccg ttttcacata ttctttatgc gaaagacttt
     1381 tatgaagcgt ggaaatatgt tccattttta ttaatagcta atgttttaaa ttgtgcatca
     1441 ggtctgttag gacctatctt atcagcaaaa aaagattcta aatccatgat gtggtctgca
     1501 atcatagggg cattagttaa tgtgattatg agtgtagtcc ttgtttataa gataggggtt
     1561 caaggtgcca caatagccac tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga
     1621 gctgtgggaa aagatatttt tataaagcat tatcatattg ttgtggaatc ttggggatta
     1681 ttgtttgttc aggcaatcat agaaatatat tttaaaaatt atgtaataga aattataatt
     1741 atgattatt
//
LOCUS       DADOZM010000237         1741 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.244, whole genome shotgun sequence.
ACCESSION   DADOZM010000237 DADOZM010000000
VERSION     DADOZM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.244"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(197..1489)
                     /locus_tag="J8I47_002845"
     CDS             complement(197..1489)
                     /locus_tag="J8I47_002845"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAZ6324237.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          494 a          361 c          308 g          578 t
ORIGIN      
        1 tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac cgtttgttcg
       61 gaaaaaggga ctgtgacacg gtttttgtca cagtccctta ttctgaataa acagtgttca
      121 agttattatt atcgtataga taaacacttc gactcgctct ggtaagaacg tcctgattca
      181 gtcgtcgttt ttatccttag atttgcgccc agacgctttc taagatgttt gtttgtgttc
      241 tatcaggtcc gacagaaaat gttgagatac gtacaccaac aagctctgag atacgacgta
      301 catagttacg tgcgttttct ggcagttctg caagtgtttt gcatcctgta atgtcttctg
      361 accagcctgg caattcttca tagattggtt tgcagcggtt caactctttc aagcttgctg
      421 gatagtggta gattagttcg ccatctaatt cataagccgt acaaattttt actgtttcca
      481 atccacttaa tacatcgatc gagtttaaag ataggttggt aatacctgaa actcgtttag
      541 aatggcgcat aactacagaa tcaaaccagc ctacacgacg cggacgacca gttgtcgtac
      601 cgtattctcg tcctacttcg cggatttgtt ctcctgtttc atcaaataat tcagttggaa
      661 atggtccatc accgacacgt gacgtatagg ctttgcagac gccgacaact ttattgattt
      721 ttgaaggccc tacaccgctt ccgattgtta ccccaccggc tactgggtta gaagaagtaa
      781 caaaaggata agttccctgg tcgatatcca gcattacacc ttgtgcgcct tcaaacaaca
      841 cgcgtttgcc tgcatccaaa gcatcattca agataacgga agtatctgtg acgtattgtt
      901 tgatttgttg tccgtattca taatattctt caaaaatatc gtcaaaatca agtggttctg
      961 aatcaaacat ttttacaaat tgacggtttt tgtcatctag attcaaacga agacgttctt
     1021 caaaaatttc tttatctaat aagtctgcaa tacggatacc tacacgagct gctttatcca
     1081 tataggcagg tccgattcct ttaatcgttg taccaatctt attttcgccc ttagcatctt
     1141 cttgtaattg gtccaatttg atatgataag gcaagattac gtgcgcgcgg tcagaaatac
     1201 gcaaattact tgtttctaca cctttttcat gtaaataagc taattcttta accaatgatt
     1261 taggattgac tacaacgccg tttccgatca cactgatctt gtctttataa aaaatcccag
     1321 aaggaatcaa atgcagttta taagtcacac cgttaaactt aattgtgtgc cctgcattgt
     1381 cgccaccttg gtaccgtgca atcacttcag cattttcact gagaaagtct gtgatttttc
     1441 ctttcccttc atcgccccat tgcgtaccta ctactactac agatgacatg tgaacacctc
     1501 gttctatatt atttaatcct gaaccattgt atcaataata gatacatatt tcaaataaaa
     1561 tcgaatgatt tttattttta ttttataaaa acgcttaaaa aacgaacttt atctttttag
     1621 aatggttatt aaaaaagagg ttgtaacaga agtggtcagc ttcaagaaat aagaaggaat
     1681 gtacgaaaat tgcttctcaa atttttgtga aatttcgact tatttttcga agaagctact
     1741 t
//
LOCUS       DADOZM010000238         1724 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.245, whole genome shotgun sequence.
ACCESSION   DADOZM010000238 DADOZM010000000
VERSION     DADOZM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.245"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            86..898
                     /locus_tag="J8I47_002846"
     CDS             86..898
                     /locus_tag="J8I47_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAZ6324238.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            895..1695
                     /locus_tag="J8I47_002847"
     CDS             895..1695
                     /locus_tag="J8I47_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAZ6324239.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          527 a          272 c          392 g          533 t
ORIGIN      
        1 aaacaatgat ttcataggaa aaacagttag atattgctat aatacttaag atagtgggaa
       61 tcttttaagt ataggaggaa aaagaatgtt acgttttttt ccgcaaaaaa acaaatatat
      121 tcaagaaaaa agcgaatttc aatttaatac cgatggacca attcctcagc acattgccat
      181 catcatggac ggcaatggtc gttgggcgca aaaccgtcgt ttgccacgag ttgctggcca
      241 taaagaaggc atggaaacag taaagaaagt cacaaaaaaa gcatcaaggc ttggagtgaa
      301 agtgttgacg ctttatgcct tttcgactga aaactggaag cgaccaaaag atgaagtaag
      361 ttttctcatg caactacctg tcgacttctt tgatacgttc gtaccagaat tgattaaaga
      421 aaatgtcaaa gttcatgtca tgggttatga aaacgtattg ccagaacata cgcaagatgc
      481 tgtaagacgt gcaattgagc aaacaaaaaa caatactgga atggtattga attttgcttt
      541 gaattatgga agcagagcag agatcgtgac tgctgtccaa gaaattgccg aagaagtagc
      601 aaaaggagaa atacacgcag aagaaatcga tgatgaattg attgctaagc atttaatgac
      661 aggtttcttg cctaaagagt tacaagatcc tgagctgatg atccgtacaa gtggagaaga
      721 gcgaattagt aattttctgt tatggcagat cgcatatagt gagctgtatt ttacaaaagc
      781 gctttggcct gattttgatg gtgcgcatct tgaagaagca atcgcctctt atcaaaatcg
      841 agaccgtcga ttcggcggtg taaaaaaaac agaagatgag aaaggggatc agtcatgaaa
      901 caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta ttccgattat atggatcgga
      961 ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa taggagttta cgagctgttt
     1021 cggatgaaag gattgacact cttaagtttt gaaggagttt tatccgccgt tggtgcagtg
     1081 ttacttgttt tgccaaaaga aagatggttt ttctttcttc cagagaatgt aagtacattt
     1141 atgttatttt atcttatagt tatgattctt ttaggaacat cagttatttc taaaaatacg
     1201 tatacgattg atgaagcagg ttttcctgtg cttgtcagct tgtatgtggg aataggtttc
     1261 cagaattttg tcaatgcacg aacagaaagc ttggctgttt tgatatttgg tctatttatt
     1321 gtttgggcaa ctgatatcgg agcatatatg gttggtcgac gttatggtca gcataagtta
     1381 agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc tcggtggtat tggtagtgct
     1441 gttgtagtcg catttcttta tttattgcta tacccagcaa aagaactatt taatcatggt
     1501 atgtttgtca tgcttctgat gacgattatt ttttcagcag tcggacaatt tggtgatttg
     1561 gtcgaatctg caatcaaacg tcattatgat gtgaaagact cgggaaatat cttaccaggc
     1621 catggtggaa tcttagaccg tttcgacagt ttgctgtttg tatttcctat catgcattta
     1681 tttggcatta tttagtaagt atacctgcgt ttctttgtga attt
//
LOCUS       DADOZM010000239         1710 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.246, whole genome shotgun sequence.
ACCESSION   DADOZM010000239 DADOZM010000000
VERSION     DADOZM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.246"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(137..1528)
                     /locus_tag="J8I47_002848"
     CDS             complement(137..1528)
                     /locus_tag="J8I47_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAZ6324240.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT          550 a          380 c          291 g          489 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaaattt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc
//
LOCUS       DADOZM010000240         1664 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.247, whole genome shotgun sequence.
ACCESSION   DADOZM010000240 DADOZM010000000
VERSION     DADOZM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.247"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            131..505
                     /locus_tag="J8I47_002849"
     CDS             131..505
                     /locus_tag="J8I47_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ6324241.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="J8I47_002850"
     CDS             complement(619..1248)
                     /locus_tag="J8I47_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ6324242.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="J8I47_002851"
     tRNA            complement(1382..1454)
                     /locus_tag="J8I47_002851"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DADOZM010000241         1662 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.248, whole genome shotgun sequence.
ACCESSION   DADOZM010000241 DADOZM010000000
VERSION     DADOZM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.248"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..752
                     /locus_tag="J8I47_002852"
     CDS             138..752
                     /locus_tag="J8I47_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ6324243.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="J8I47_002853"
     CDS             complement(776..1126)
                     /locus_tag="J8I47_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ6324244.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="J8I47_002854"
     CDS             complement(1120..1383)
                     /locus_tag="J8I47_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAZ6324245.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          559 a          294 c          256 g          553 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa at
//
LOCUS       DADOZM010000242         1633 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.249, whole genome shotgun sequence.
ACCESSION   DADOZM010000242 DADOZM010000000
VERSION     DADOZM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.249"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="J8I47_002855"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="J8I47_002855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAZ6324246.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          562 a          283 c          378 g          410 t
ORIGIN      
        1 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaa
//
LOCUS       DADOZM010000243         1626 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.250, whole genome shotgun sequence.
ACCESSION   DADOZM010000243 DADOZM010000000
VERSION     DADOZM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.250"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            378..1508
                     /locus_tag="J8I47_002856"
     CDS             378..1508
                     /locus_tag="J8I47_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ6324247.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          465 a          267 c          347 g          547 t
ORIGIN      
        1 ttcaaattcc attgcctaat cattaggaaa agtctatcat gaactcgtaa atagaatata
       61 gtaataacct tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga
      121 aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat
      181 gttttcttta cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt
      241 cttgttcgaa aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag
      301 acaaactcgc tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa
      361 agatcgattg gtggattttg ggtccttatt tgactttgtc catgatcggt cttttagaag
      421 tatacagtgc cagttcttat cgtttattgc aagccgatga aaatacaaaa agtctattat
      481 tgcgtcaact aattttcata tttttgagtt ggagcgtgat cttcttagct cgttcagtca
      541 aactacacta tttacttcac cctaaaatag caggatacgg tttagcctta tcgattttct
      601 ttttagtatt agtaagaata gggatattcg gtgtcactgt caacggcgca caacgttgga
      661 tctctctgtt tggcattcaa ttccagcctt ctgaactggc aaatcttttt ttgatttttt
      721 atttaagctg gttttttcgt gacggaaata gtagcccaaa agatctaaaa aaaccattcc
      781 tgattacagt aggtataact tttctgattt tatttcagcc aaagattgct ggagcattga
      841 tgatcctttc gattgcgtgg gtcatatttt gggcagcggc ggttccattt aaaaaaggga
      901 tctatctaat cgttactttt tctgcattgc tgattggagc agcaggcggg gtattatatt
      961 taggaaataa aggttggctt ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt
     1021 taagagattc ttttatcgat agtcatggtg cgggctatca aatgacccat tcgttttatg
     1081 cactatataa tggtggcata tttgggcggg gattaggaaa tagtatcaca aaaaaagggt
     1141 atcttccaga gacagaaaca gattttattt tttcgattat tacagaagag cttgggctaa
     1201 tcggtgcatt gtgtgtgctt ttcttattgt tctcattatg catgagaatc ttttgtttaa
     1261 gcagtcgctg taaaaatcag caagctggat tgtttctttt aggattcgga actttgctat
     1321 tcgttcagac gataatgaac gtgggaagta tcgcggggct gatgccaatg actggtgtgc
     1381 cattgccatt tgttagctac ggaggaacaa gttatctgat cctttcttta ggaatcggaa
     1441 tcacgttgaa tatttcttca aagatacagg ccgaagagtt ggcgctctat cgacctgaaa
     1501 aacaataaag agaaactgag aaactctcag tttgaattat cataaagtac aatcttttat
     1561 ttttcaagcg agaggttctt ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc
     1621 atatta
//
LOCUS       DADOZM010000244         1621 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.251, whole genome shotgun sequence.
ACCESSION   DADOZM010000244 DADOZM010000000
VERSION     DADOZM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.251"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            14..151
                     /locus_tag="J8I47_002857"
     CDS             14..151
                     /locus_tag="J8I47_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAZ6324248.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            188..481
                     /locus_tag="J8I47_002858"
     CDS             188..481
                     /locus_tag="J8I47_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324249.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            478..702
                     /locus_tag="J8I47_002859"
     CDS             478..702
                     /locus_tag="J8I47_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAZ6324250.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            699..>1621
                     /locus_tag="J8I47_002860"
     CDS             699..>1621
                     /locus_tag="J8I47_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ6324251.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT          550 a          269 c          343 g          459 t
ORIGIN      
        1 ggaggtgagt agtatgttta gttttagcga tgtgaaaatg atgtatgatt ggggctgttt
       61 tactgacgat caagttcgac tattcgttcc actatgcatt acagacgaag aagcagataa
      121 aatcattaat aaagataaga gcgcatctta attgatgcgt ttttatttaa ggtaaaggag
      181 ttgtcacatg attaatttag gggaatgggg aatgatagca ggatcaataa ccgctatcgt
      241 ttctttgatt ttattagtaa ttagaccgat tgctgcatct ttctcgaaaa ttactgagac
      301 tctttcaaaa gtaagccaca atttagattt gctgactaaa gatttagaat cgagcaaatc
      361 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatac
      421 agaaaaattg gtggaacaca cgcaacaaat caaaactttg tttagggaga gaagaaaatg
      481 aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct
      541 actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc
      601 atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac
      661 aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta
      721 ctgcgatgag cctgttaacg gctctttttt tattgccaat taacggattt gcctatacta
      781 tcaacaatga atttaatttg ggcgcaaatg aaggtagctc acaagtagca aataatcagt
      841 atattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
      901 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
      961 accaagtcgg acaacctggt tatgtacagt acggtgctgg ttcatatgct aatgccaata
     1021 gtcctgtgca gattgagtta caacacacac atgataaagc aacttttgaa aaaaactata
     1081 aagcatacgt tgaattggct agagattcag caatgaaata tggtattcca ttaacattag
     1141 acacgcctta taaccaacca ggaatcaaat cacatttatg ggtaactcaa aacatttggg
     1201 gcgatcacac tgatccgtat ggatacttat cagaaatggg tgtaagtaag gaaaaactgg
     1261 cttacgattt agctcatgga tttacagacg aaaacccaac gacttctgag aacaagcctg
     1321 tcattgatcc aacccgagca ggtgcagcaa atccaacttt atcagatgga acgaaccatt
     1381 ctcacattga tcagtttgga gaaatcgaaa atgcgaactt acacgtcgct ggatggcaca
     1441 tcgctaacta taagtacgag tatattttca ttatggacta caatactggg aaagaattag
     1501 ctcgagtaag agctgatgga atttatagac cggatgtaaa tcaagcttat aatacttctg
     1561 gaaatgttgg ctatcatgta tcttttaaca tgcgtaactt ccctaataag aaagtctatg
     1621 t
//
LOCUS       DADOZM010000245         1602 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.252, whole genome shotgun sequence.
ACCESSION   DADOZM010000245 DADOZM010000000
VERSION     DADOZM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.252"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            91..963
                     /locus_tag="J8I47_002861"
     CDS             91..963
                     /locus_tag="J8I47_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ6324252.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1259..>1602
                     /locus_tag="J8I47_002862"
     CDS             1259..>1602
                     /locus_tag="J8I47_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6324253.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT          497 a          308 c          360 g          437 t
ORIGIN      
        1 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
       61 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc
      121 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
      181 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
      241 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
      301 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
      361 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
      421 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
      481 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
      541 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
      601 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaacgc
      661 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
      721 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
      781 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
      841 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
      901 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
      961 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
     1021 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
     1081 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg
     1141 tattgtgcaa tagtttttta cacgccgttt gtataattaa gcgagacaaa taaaaaagcc
     1201 atttatgtta cactcaaatc gtaatacccg ctaaagcata caaggagagt gaacataaat
     1261 gacttatacc catcttacac caaacgagct tgtaatgata gaagcatatt ttcatcaaga
     1321 aactccggtt gctatcgttg cgaagcagct taaacgtgga cgccaaacaa tttacaatgt
     1381 ctataacttt ctcaaatgtg gtggaacagc acttgaatac tttgaacaat acaaagaaaa
     1441 taagcgacgt tgtgggagaa ccgaaatcat ttttcctgct gaggaaaaag aatacattgc
     1501 aaaaagatca actgaaggtt ggaccccaga cgtcattatt ggccgtgcag agcgaacctt
     1561 ctcttgttca gtaagtaccc tctatcgccg gtttaagacg gg
//
LOCUS       DADOZM010000246         1548 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.253, whole genome shotgun sequence.
ACCESSION   DADOZM010000246 DADOZM010000000
VERSION     DADOZM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.253"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            19..1236
                     /locus_tag="J8I47_002863"
     CDS             19..1236
                     /locus_tag="J8I47_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ6324254.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1249..>1548
                     /locus_tag="J8I47_002864"
     CDS             1249..>1548
                     /locus_tag="J8I47_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ6324255.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERED"
BASE COUNT          548 a          243 c          323 g          434 t
ORIGIN      
        1 cgctggaggt gttcaaacat gagcaatgat acacaaccag aaatccgttt cccaggattt
       61 acagatgatt gggaacagag gaagttgggg gacttcgttg tagattatgt tgaaaagaca
      121 agtgtacaaa atcaatttcc tatgctaact tcatctcaac aaaaagggat agttttgcaa
      181 gaagactatt ttgcaaatcg gcaagtaact acagaaaata atattggtta ctttgtacta
      241 cctagaggat atttcacgtt ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt
      301 aatgacatta ttgatagagg gattataagc tatttttatc ctgtatttac tttaaaatct
      361 gctgattccg acttcttctt aagacgtatt aacaatggaa tccaacgaca attatctatt
      421 caagcagagg gaacaggtca acatgttctg tctctaaaaa agtttaaaaa tatagtagca
      481 atgtttccta gtgaggaaga acaacaaaaa atcggcacct tcttcaaaca acttgacgac
      541 acgatcgctc ttcatcagcg taagttagat ttgctgaagg aaacgaaaaa aggattctta
      601 caaaaaatgt tccctgaaaa cggagcaaaa gtgccagaaa tccggtttcc tggttttacg
      661 gaagattggg aagagcgtaa gttgggagaa ttaggtaaaa ctcaatcggg aattggattt
      721 cctgatgccg agcaaggtgg ttctgaaggt actccatttt ttaaagtttc cgacatgaat
      781 aacattggta atgagtatga aatgagaaat gcaaatcatt atgtatctaa tgagcaaatt
      841 gagcgtaaaa aatggaagcc aataaaagat gtgccagcga ttatatttgc taaagttggc
      901 gcagcaataa tgcttaatag aaaacgtttg gtcacaagcc ctttcttaat agataacaat
      961 accatggctt atttatttaa taatacttgg gatatctatt ttggtaaaat attgtttgaa
     1021 actataaatt tgccaagata ctcccaagta ggggcattgc cttcttataa cagttctgat
     1081 attgagaata tcagtgttaa ggttcctgta aaggatgaac aacaaaaaat cggtactttc
     1141 ttcaaacagc tagatgatac gatcgctctt catcaacgta agttagattt actaaaagaa
     1201 acgaaaaaaa gcttcttaca aaagatgttt gtctaggagg gaagaagaat gaaaattagt
     1261 caccgtgagg aagcagaagt cgaagaacag ttgattcgtg tacttggaga agggcataat
     1321 cagtggacgt atcgtccgga tctaaaatca gaagaagacc tttgggtcaa tttacgccaa
     1381 aaaattatct caaataatca agcagaactg aatgattttc ctttgaccga taaagaattt
     1441 gaaacgatca aaacggaatt actgctgcga acgaaaaccc catttgatgc tgcgaaatgg
     1501 ttaaaaggtg aaaatgggat ggctcggatc acgattgaac gggaagat
//
LOCUS       DADOZM010000247         1543 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.254, whole genome shotgun sequence.
ACCESSION   DADOZM010000247 DADOZM010000000
VERSION     DADOZM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.254"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            30..1496
                     /locus_tag="J8I47_002865"
     CDS             30..1496
                     /locus_tag="J8I47_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAZ6324256.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          426 a          289 c          327 g          501 t
ORIGIN      
        1 tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg
       61 ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt
      121 acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac gggggacttg
      181 gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt
      241 ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct
      301 tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta
      361 cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt
      421 ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga
      481 ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg
      541 gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat
      601 tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga
      661 atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg
      721 ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg
      781 tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga
      841 cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc
      901 ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg
      961 ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat
     1021 cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac
     1081 ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat
     1141 tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc
     1201 cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag
     1261 ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta
     1321 tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca
     1381 caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta
     1441 tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa
     1501 tgaacataaa aacaggaatt gcttatttgc agcagttcct gtt
//
LOCUS       DADOZM010000248         1525 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.255, whole genome shotgun sequence.
ACCESSION   DADOZM010000248 DADOZM010000000
VERSION     DADOZM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.255"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            360..1400
                     /locus_tag="J8I47_002866"
     CDS             360..1400
                     /locus_tag="J8I47_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAZ6324257.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          604 a          196 c          237 g          488 t
ORIGIN      
        1 atatatacta ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc
       61 ataaggtagt tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat
      121 tttaaaacta aattttattc atacgttttt ctggtagtat ttaattataa ataatattta
      181 tttaaagtag ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta
      241 ggtagtattt aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat
      301 tttcgaaaag agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa
      361 tgactgattt taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc
      421 caaaagtctt tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct
      481 atatgctttt aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag
      541 gtaatgttta ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac
      601 aaaaagcgat agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga
      661 tgggatttaa caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa
      721 aagtctcaga aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg
      781 ttgataaatc agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat
      841 cccttggggc tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg
      901 gtatgaaaat cataccagaa cttaataata atatattaga cactaataga cacaatatag
      961 acactgaaaa agaccgccta caagatcaat tgttgttaga caattttgag acaattatga
     1021 caaacgacag cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt
     1081 cttcaagtta cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag
     1141 ctgaaataga aagtggtatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg
     1201 cagaacaaga attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag
     1261 tcgaaaatat ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa
     1321 tagctgctaa agtatcaagt gaaaaacgtt tgaattatga aagctcccca agcactatta
     1381 cgaaagactg gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca
     1441 ttaaaattaa aaatttttta ttattctaat tatattacga atattacaaa ataatagcta
     1501 ttttaataga atttataagt taaaa
//
LOCUS       DADOZM010000249         1522 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.256, whole genome shotgun sequence.
ACCESSION   DADOZM010000249 DADOZM010000000
VERSION     DADOZM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.256"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            158..496
                     /locus_tag="J8I47_002867"
     CDS             158..496
                     /locus_tag="J8I47_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324258.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(701..1276)
                     /locus_tag="J8I47_002868"
     CDS             complement(701..1276)
                     /locus_tag="J8I47_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAZ6324259.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          474 a          313 c          258 g          477 t
ORIGIN      
        1 ttaatttgac aattaagaaa ctaaaaaaat aatccaagaa acattgaacc tatggatcca
       61 aaagctaccc aaatactata gctaaccccc cgctaccttc aattttgcag ataaaacgct
      121 ttgggagaaa atttcaaaga caacaggcca tcctatcatg cccgattata aacaaaaagc
      181 tggctattcc ttttgctctg acaaatttca attgggaaag ttcacgaaac tagcaggtga
      241 agaagtttca actgttcgga tcaaagaatg cccgcttcaa atagaaacat tggttcaacg
      301 aatttcaact agacaagact ttgctattgt agaacgtgag atcgtagcta tttttgtaga
      361 agaagggatt ttacatgatc caactcatat caatgtggat aaatggcaac ctcttatcta
      421 taaattcaga gaatatacaa ctgccaacca gtcgttaact ttgaatttta gattccaaga
      481 attcaaagag tcataatgat aaacatcgtt aggtataaat aaaaaagact cgcagcacga
      541 gtaaaaaaca aatagaagct atacattaga aaggtcgact gtgaccctat agatgcagta
      601 tatgcagctt atgagcattt cttctgttct ctttgttttt gggatctgtg ctgaagtctt
      661 tttcgttttt tgccgctctt tctttgaaaa atcgattatg ttataccttc tccattacta
      721 attcaggcat ctgctgagta atgatttttc gcgcaaaaga aatatctgtt acccaaggtt
      781 cgatttgttc ttcagtcaag acaagcggca ttcggtcatg gattggtgcg actgtgcggt
      841 taggttcagt agtcattatg attgattctg ccattttttc atcctgatgg ctacgataaa
      901 aaccgccaat atacactatt ttttccttat ttgaaaacat aaatttgtct ttttcttcat
      961 tccactcata aaagccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa
     1021 aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa
     1081 atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta
     1141 acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg
     1201 gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa
     1261 aataacgacc acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag
     1321 aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga
     1381 caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca
     1441 acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc
     1501 ttacgacctc tgggtaaacg gt
//
LOCUS       DADOZM010000250         1515 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.257, whole genome shotgun sequence.
ACCESSION   DADOZM010000250 DADOZM010000000
VERSION     DADOZM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.257"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..355
                     /locus_tag="J8I47_002869"
     CDS             <1..355
                     /locus_tag="J8I47_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324260.1"
                     /translation="TLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRFGF
                     ETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQM
                     SESKFTSIFNDVNLR"
     gene            576..1515
                     /locus_tag="J8I47_002870"
                     /pseudo
     CDS             576..1515
                     /locus_tag="J8I47_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          545 a          222 c          352 g          396 t
ORIGIN      
        1 gactttagca gatattaaag cgcaagaaga aaaattaaaa aaagaacaag cgaaacttcg
       61 ccaacagaaa aaacgcttga ttgagaaaga aaatgctgca atgggcaagg ctcttcggaa
      121 gcgttttggg tttgaaactt ttgctgattt tgagcggtgg ctatcgtcga tttctgacga
      181 tcagcaacac gaaaaatcag tggataatga gtggcttgct gatgaaaaat cagcgccaag
      241 cgaacgagat tatcccgatg aatctgacgg cttattcgga gtgataccga ataagaaaag
      301 tcagatgagt gagagtaaat ttacgtcaat atttaatgac gtgaatttac gataagcgat
      361 tattttgcgt gaagcaaaat aaagcgtaaa aggagttgag agcgtgacac gctttcggct
      421 ccttttttcg ttgtcacttg tgacagcgaa taccctcgga gagcatttgg aattgtgaca
      481 gccatgtcac aattaggaaa atgttactct ttgacaaaga gtaacagaaa ggtttacact
      541 ctttttatta gcgacagccg aaggagggtt ttaacttggg gttcagtatt tcgttcaaaa
      601 agggaacaaa gaatacaagc atttctcata ataatcgtga gttaactgat agccaaaaaa
      661 tcaatgactg gcataagcat attgatttca caaaatcgga tgaaaatatc tatttagaac
      721 aaacaaatat tcgtgaaaaa tatgaagaat tattcggtga agccgtagaa aaatataatg
      781 caaagcagaa aagagccgat agaaaaatcg aggactattt agcaaaggtt cgcaaggata
      841 aaaaattgga accacagcga gaatttatcg tgcaaattgg tactcttgat gattttcgta
      901 ctactcgtga tgatggttct tctactggca tatcggaaca acaagcagaa caaaatcgag
      961 tgattgccaa caaaatttta gttgaatatt tcaaagaatt taaagaacgg aatcctaatt
     1021 tatccgtcta caatgctgtt attcataatg acgaagtaag tccgcacctg catttgaata
     1081 ttgttcctgt tgctgaaggg tataaacggg gtgttcaaaa gcaaccaagt tttaacaaag
     1141 ccttgcaaca gcagggttta cgagcagata aagagaatgg tcgagtgatt tggaataatt
     1201 ttaggaatca ggaagttgag tcaattgaac gtttgatggc agattttggt tgggagcgtg
     1261 agttagtcgg tactaacaaa atcaaagatg ttcacgaata taaagaaatc atgagcgaga
     1321 tttcagaatt gcgtcaaacg gccgtaaaag agcgtgagag cgtttctgat gagttgaggt
     1381 atatttttct aggcaaagag aagaaaatga gcgagaaaag gctctggtgg cttctgagag
     1441 agtgaagatt gaagaagaga agaaaaatat gctgaatgta aaaaaagaga tggcggtcat
     1501 tttccaaaaa gatat
//
LOCUS       DADOZM010000251         1514 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.258, whole genome shotgun sequence.
ACCESSION   DADOZM010000251 DADOZM010000000
VERSION     DADOZM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.258"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..300)
                     /locus_tag="J8I47_002871"
     CDS             complement(<1..300)
                     /locus_tag="J8I47_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ6324261.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREN"
     gene            complement(314..>1514)
                     /locus_tag="J8I47_002872"
     CDS             complement(314..>1514)
                     /locus_tag="J8I47_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ6324262.1"
                     /translation="PEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSGIRFLRA
                     RDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLITDDNPI
                     YFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGKKTPISL
                     PTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEVRFPGFT
                     GDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLELEEDKYA
                     NKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDKEKIKES
                     TNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLLKETKKG
                     FLQKMFV"
BASE COUNT          417 a          325 c          234 g          538 t
ORIGIN      
        1 attttcccgt tcaatcgtga tccgagccat cccattttca ccttttaacc atttcgcagc
       61 atcaaatggg gttttcgtcc gtagcaataa ttcggttttg atcgtttcaa actctttatc
      121 ggtcaaagga gaatcattca gttctgcttg attatttgag atgatttttt gacgtaaatt
      181 gctccaaaga tcttcttctg attttagatc cggacgatac gtccactgat tatgtccttc
      241 cccgaggaca cgaatcaact gttcttcgac ttccgcttcc tcacggtgac taattttcat
      301 tcttcttccc tccctaaaca aacatctttt gcaagaagcc ttttttcgtt tccttcagca
      361 aatctaactt acgttgatga agagcgatcg tatcatctag ctgcttgaag aaggcaccaa
      421 ttttttgttg ttcatttata tccggtggaa actgaaatgt cctttgttct ataccacctt
      481 tagtcacatg taccattgtc gttccgttag ttgattcttt aattttctct ttatcagtca
      541 atagccaagt atatagatac tgtttgtcca attccttaga taaaaattta agtttccaaa
      601 tatgataatg gtagattaca cgttcttcat tccacatttc aggaccgaag cttgttgccc
      661 ataaatataa gagatctcct ttattagcat acttgtcttc ctctaattct aagtttgaat
      721 aataccatct atcattagta ttaaaattac ctactcttaa aaccctatat tttccagaat
      781 caagtaattc ttgttgttta aaagctctac cattcaaaaa aactaccact tcccccaact
      841 tccgctcttc ccaatctccc gtaaatcctg gaaaacgaac ttctggcact tttgctccgt
      901 ttttagggaa cattttttgt aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt
      961 gctggtgaag catgatggtg tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt
     1021 cgtcataagt tggaagtgag ataggtgtct ttttaccact atcaattgta taggttccga
     1081 cggttccaat gcctgcaacg tcacggatat acttctgaat tttgttattt atgaacgagt
     1141 agtaaaagaa gtttccgtca attttatgct cattcttgaa ccaaatgatg tttccatcct
     1201 taaagtaaat tgggttatca tcagtgatta ataaggggac acctattgat ccgacaccag
     1261 ttacaagcaa gtctccttga gaaactttac ctgaattttt cgaatattca ttatattttt
     1321 cttctgaaat gtaaagataa tcggatggtt cctcattttt tgcagctgag acaatatcac
     1381 gagcacgtaa aaatctaata cctgagttag tccaatctga ttgatgaatt cgcttcacag
     1441 aagtaacatc catcatatct cctaacttac gctcttccca atcttccgta aaaccaggaa
     1501 accggatttc tggt
//
LOCUS       DADOZM010000252         1502 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.260, whole genome shotgun sequence.
ACCESSION   DADOZM010000252 DADOZM010000000
VERSION     DADOZM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.260"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            264..1457
                     /locus_tag="J8I47_002873"
     CDS             264..1457
                     /locus_tag="J8I47_002873"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAZ6324263.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT          470 a          244 c          346 g          442 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ct
//
LOCUS       DADOZM010000253         1485 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.261, whole genome shotgun sequence.
ACCESSION   DADOZM010000253 DADOZM010000000
VERSION     DADOZM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.261"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            287..694
                     /locus_tag="J8I47_002874"
     CDS             287..694
                     /locus_tag="J8I47_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324264.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="J8I47_002875"
     CDS             708..1109
                     /locus_tag="J8I47_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324265.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="J8I47_002876"
     CDS             1111..1482
                     /locus_tag="J8I47_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324266.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          473 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatga
//
LOCUS       DADOZM010000254         1480 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.262, whole genome shotgun sequence.
ACCESSION   DADOZM010000254 DADOZM010000000
VERSION     DADOZM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.262"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          551 a          169 c          303 g          457 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat
//
LOCUS       DADOZM010000255         1472 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.263, whole genome shotgun sequence.
ACCESSION   DADOZM010000255 DADOZM010000000
VERSION     DADOZM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.263"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>1472
                     /locus_tag="J8I47_002877"
     CDS             <1..>1472
                     /locus_tag="J8I47_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ6324267.1"
                     /translation="RDRRFDVTLLINGLPIVQIELKQVTAKDGVYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNEKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAIQALFIAANKHQSGYVWHATGSG
                     KTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTKFASEFNTGISSGNAKANS
                     LIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQH
                     IVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYG
                     EVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRM
                     IDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSS
                     IDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPD"
BASE COUNT          512 a          267 c          307 g          386 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
       61 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
      121 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
      241 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
      301 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc
      421 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
      481 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
      541 acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa
      601 tttgcttcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc
      661 gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat
      721 gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa
      781 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cgtgtttgta
      841 gtagatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt
      901 ccgaaatcca cttggtttgg ctttacaggt acaccgattt ttcctgagaa tcggaaacaa
      961 gccaaagggc aattggcacg tacaacccat gatcagtatg gagaggtctt gcatacctat
     1021 accattaaga atgcgctaga agatggctct gtcttaggat ttcaagtaga gcatgaaaac
     1081 accgtggagc cgacttcact agagaataaa atctatcgga aattaaaaga agtagaaaca
     1141 tatgcagagt attcatcaga acagatcaat cgtatgattg atcaaatgga acctgtcaaa
     1201 aaagaaagtt atttggaccc agctgttttt gaagcagatg aacatatcca aaaagtcatt
     1261 cataaaatgt tccgtccaga taatgcctat acaaaatttg attttcgaaa tggcaggcca
     1321 acgaaatctg caatcttaac cacgagttcc attgacatgg ccaaaaagta ttatcgggcc
     1381 atcaaagaga tgacgaaaga gcctgattgg ttaacaaaag agtttcccaa tcagctcatt
     1441 cgtgaaggac gaacgatgga ggatcctgat tt
//
LOCUS       DADOZM010000256         1472 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.264, whole genome shotgun sequence.
ACCESSION   DADOZM010000256 DADOZM010000000
VERSION     DADOZM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.264"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>1472
                     /locus_tag="J8I47_002878"
     CDS             <1..>1472
                     /locus_tag="J8I47_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ6324268.1"
                     /translation="RDRRFDVTLLINGLPIIQIELKQVTAKDGFYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNKKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAIQALFIAANKHQSGYVWHATGSG
                     KTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTKFASEFNTGVSSGNAKANS
                     LIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQH
                     IVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYG
                     EVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSPEQINRM
                     IDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSS
                     IDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPD"
BASE COUNT          496 a          264 c          320 g          392 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttattg atcaatggct taccgattat ccaaatcgag
       61 ctaaaacaag taactgcaaa agatggtttt tatcaggcgt ttaatcaaat caaaaaatat
      121 gctgaagaag gcatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa cgactcggta ttttgcaaat gccttaccga aggatttgca tcctaagttt
      241 ttatttagtt ggcgaacgaa agataataaa aaggtagaaa acttatatga gttttgcaaa
      301 caagtgctaa atatcccaga tgcccatcgc ttgattgcgg attatacgat tgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagtgcatgc gattcaagcc
      421 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
      481 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
      541 acgatcatgt tagtggaccg caaagattta gataatcaga ccaccacaga gtttacgaaa
      601 tttgcttcgg agtttaatac tggggtttct tctggtaatg ccaaagcgaa tagcttgatc
      661 gtgggaacag gaagtgccaa agagttaagt gaaactctgt tggcagatgc caatgcgaat
      721 gttgtgatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacag
      781 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg ggcaacatat cgtgtttgta
      841 gttgatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt
      901 ccgaaatcca cttggtttgg ctttacaggt acaccgattt tccctgagaa tcggaaacaa
      961 gccaaagggc aattggcacg tacgacccat gatcagtatg gagaggtctt gcacacctat
     1021 accatcaaga atgcattaga agatggctcg gttttaggat ttcaagtaga gcatgaaaac
     1081 accgtggagc cgacttcatt agagaataaa atctatcgga aattaaaaga agtagaaaca
     1141 tatgcagagt attcaccgga acagatcaat cgtatgatcg atcaaatgga gtctgtcaaa
     1201 aaagaaagtt atttggaccc tactgttttt gaagcagagg aacatatcca aaaagtcatt
     1261 cataaaatgt tccgtccaga caatgcctat acaaaatttg attttcgaaa tggccgtcca
     1321 acgaagtctg cgatcttaac cacgagttcc attgatatgg ccaaaaagta ttatcgggcc
     1381 atcaaagaga tgacgaaaga atctgattgg ttaataaaag agtttcccaa tcagcctatc
     1441 cgtgaaggac gaacgatgga ggatcctgat tt
//
LOCUS       DADOZM010000257         1472 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.265, whole genome shotgun sequence.
ACCESSION   DADOZM010000257 DADOZM010000000
VERSION     DADOZM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.265"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            244..1347
                     /locus_tag="J8I47_002879"
     CDS             244..1347
                     /locus_tag="J8I47_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAZ6324269.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          482 a          240 c          328 g          422 t
ORIGIN      
        1 taccgagaaa atggcttttg aacaccgttt attcaaaaaa agggactttg acactacttt
       61 tgtcaaagtc ccatcggttt tttatctatt tttattacaa tcatttcttc cagagtagtg
      121 acatctggaa tggctgaaat gtaaacagat tatttgcctg tatcaatgtc gaagagtaat
      181 ataaaaagtg taaacgctat catattgttc atcattcaaa ttttatagag agaaggcatt
      241 gagatggaaa aaacatatca agcaggtaca aatgaaggaa tagtagattt tatcaatatg
      301 gaagatttag aaatcgcggc ttcacaagtg attccagctg gtggttatgg ttacatcagc
      361 agtggtgctg gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcgtttg
      421 attattcctc atgtattaag agatgtagaa ttacccgata caactacaca ttttgacgaa
      481 gaaatgctga ctgcaccaat catcatggca ccagttgctg ctcatggatt agctcatgtg
      541 aaggcggaga aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt
      601 tcttacgctt cttgtacttt agaagagata agagaagcag gcggggagaa agcaccgcaa
      661 tggtttcaat tttatatgag caaagataat ggaatcaatt tggatattct tgaagtggcc
      721 aagcgtaatg gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt
      781 gaaacagacc gcagaaacgg gtttaccttc ccgctgccaa tgccgatcgt tcaggcttat
      841 caatcaggag taggccaaac gatggatgct gtatataaat cttccaaaca aaaacttagt
      901 ccaaaagatg tagaatttat cgctgctcat tctgatttgc cagtctacgt gaaaggtgtt
      961 caatcagaag aagatgtcta tcgatcatta gaatctggtg ctggtggaat ttgggtatct
     1021 aaccatggtg gccgtcaatt agatgggggg cctgctgcgt ttgattcgtt gcaatatgtg
     1081 gcagaagcag tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgtggacaa
     1141 catgttttca aagcaatcgc atcaggtgcc gatcttgttg ctattggcag acccgttatt
     1201 tatggattgt ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca
     1261 gaattggaaa tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata
     1321 aaattgcgtg agaatcgttt tatttaagaa ataaatgacg aaaaaaatag gagtgcggca
     1381 gatgatgccg tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac
     1441 gaaaaaataa aattgaaata ttttttcata ct
//
LOCUS       DADOZM010000258         1472 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.266, whole genome shotgun sequence.
ACCESSION   DADOZM010000258 DADOZM010000000
VERSION     DADOZM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.266"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..391
                     /locus_tag="J8I47_002880"
     CDS             <1..391
                     /locus_tag="J8I47_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324270.1"
                     /translation="IYHQGNSKELEVVLRYNLFSFTSEFYFLRHKERIVGMVSIIVNP
                     FQNLSYSIVNYLICEPAYTTTILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPYL
                     KDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            455..790
                     /locus_tag="J8I47_002881"
     CDS             455..790
                     /locus_tag="J8I47_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAZ6324271.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            1214..>1472
                     /locus_tag="J8I47_002882"
     CDS             1214..>1472
                     /locus_tag="J8I47_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324272.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYK"
BASE COUNT          566 a          163 c          243 g          500 t
ORIGIN      
        1 tatttatcat caaggaaata gtaaagaatt agaagttgtc cttagatata atttattttc
       61 gtttacgagt gaattttact ttttaagaca taaagagaga attgtaggaa tggtttctat
      121 aattgttaat ccatttcaaa acttatccta cagtatagta aattacctca tatgtgagcc
      181 tgcatatact acgactattt tgacaacagt attagaaaat attaacgaag tttcaatttt
      241 agaagtaaag aagctagtga ttcatttgct aaattcaaca gaagatgaat cactaatccc
      301 ctatttaaaa gattcacatt tttttaaaga agcggtacta tctaaagaat atagagagca
      361 tgatgttttg agatacgctt acatttatta aataaaattt ctgatagtat tttaataatt
      421 cggatcatat cataagatat aggagggaaa agaagtgatt ttattgattt tgtctctcgt
      481 attactagtt gtatctaatc taatgaaaaa taatcctcct acaaaaatta attcttttta
      541 tggctttaga actaaaaagt caatgaaaaa tgaaaaaagt tggcatttag cacaaaaatt
      601 gttgggggat ttttcaaaaa aaaaagttat cccagtattt ttgatgggta ttgttttttt
      661 cagtatagag atttatagtt tagctatttt aaaaaatgaa aattttttcg ttagtgtatt
      721 aatgggtgaa agtttaatcc taatactgat tttgtgttta attgttcatg aagtgaataa
      781 acaattgtga attaaaaaaa taaaaaccta gtataatcac tttaataaat tgttggtaga
      841 aggtgatcaa aatgggagta aacgaaatga tgaaagtcag attgagtttt taaatgatac
      901 cttcctcaga acttcaaaac gaattcaaag attcgttgac aaatcttgag caagatcatt
      961 taaaaaaata ttttctcttc atttgacgaa aaatgtttag aaatcttata taagtgaaga
     1021 taaagtaatt ggtgtaatta attgtagaga atagcctttg agcaattgaa gcgatacaat
     1081 tatttagaaa atgaacttcc tccagcatac tacatgattc cttacgggac gatgttaaag
     1141 aagaaagcaa gaaattttag aagtattagt agataaacta atgggctttt ctcaatttaa
     1201 gggagtagcc caaatgttta tcaaatatgg tgcagataca gtagcaacca tgtttacaga
     1261 agaatatcaa gtagactatt atgaagtaat agataaatgt gtacattttg tttatgaaaa
     1321 agtgtggaat tttatgaatt ttgcgataag tgaagaaatc ttacgagtac aagaaaacag
     1381 aacaggaata tttaccagag acagaaagct taatttgatt gcgcattata tacggaatcg
     1441 ctggtcatca ataccaaatt ttaattataa aa
//
LOCUS       DADOZM010000259         1452 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.267, whole genome shotgun sequence.
ACCESSION   DADOZM010000259 DADOZM010000000
VERSION     DADOZM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.267"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..625)
                     /locus_tag="J8I47_002883"
     CDS             complement(<1..625)
                     /locus_tag="J8I47_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAZ6324273.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="J8I47_002884"
     CDS             complement(784..1050)
                     /locus_tag="J8I47_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324274.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="J8I47_002885"
     CDS             complement(1040..1396)
                     /locus_tag="J8I47_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ6324275.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aa
//
LOCUS       DADOZM010000260         1451 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.268, whole genome shotgun sequence.
ACCESSION   DADOZM010000260 DADOZM010000000
VERSION     DADOZM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.268"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            105..1406
                     /locus_tag="J8I47_002886"
     CDS             105..1406
                     /locus_tag="J8I47_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAZ6324276.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DADOZM010000261         1429 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.269, whole genome shotgun sequence.
ACCESSION   DADOZM010000261 DADOZM010000000
VERSION     DADOZM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.269"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..365
                     /locus_tag="J8I47_002887"
     CDS             <1..365
                     /locus_tag="J8I47_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324277.1"
                     /translation="FIPEDFQNLQLENMRLEDLFRLADDDKYRFEVLKIKKNELLNKL
                     YPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEVI
                     IKYMKPVIKLVNKNKGNG"
     gene            471..734
                     /locus_tag="J8I47_002888"
     CDS             471..734
                     /locus_tag="J8I47_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ6324278.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            734..1006
                     /locus_tag="J8I47_002889"
     CDS             734..1006
                     /locus_tag="J8I47_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ6324279.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1083..1412
                     /locus_tag="J8I47_002890"
     CDS             1083..1412
                     /locus_tag="J8I47_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ6324280.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          553 a          168 c          279 g          429 t
ORIGIN      
        1 aatttatacc ggaagatttt caaaacttac agttagaaaa catgcgttta gaagatttat
       61 ttagattagc agatgatgat aagtatagat ttgaagtttt gaaaattaaa aagaatgaac
      121 tattgaataa gttatatcct gagaatgcga taaaagcaca ttttggtgat gctaatcata
      181 tgtatagttg gttcattcct aaaagattta gagggaaaat caatgctgga gatcaagtga
      241 aagtactcaa tcgaaatagt acagatgtcg taatgataac agatatctac gtagaagatg
      301 aagttataat caaatatatg aagcctgtga taaaacttgt taataaaaat aaaggaaacg
      361 gataacactt tacaatgctg ttttggtact ccaatttaaa caaaacaatc gaatttacgt
      421 acttaattaa gtacgttaga atggagatat aaattagagg gggagtagat atggcaaaaa
      481 cagtcttaaa tcctagctca gcacggaaaa atttttataa gttgttgaaa gatgtaaatg
      541 agaatcatac ggaaattgaa atcattagtg agcgcagtga aaataatgcg gtgcttattg
      601 gtttggatga ttggaaagca atcaaagaaa cgctattact tgagcaaata ggcactttgg
      661 gtgttgttcg taagcgagaa aaagatgata gtggatttac taatgttgat gaaattgatt
      721 gggataactt ataatgaata actattctgt gatgattaaa aactcggcta aagctgattt
      781 aaagaaaatc aaacagtcta atttgaaacc tcaatttgag aaggttattc agactttaaa
      841 ggaagatccc tatctcccta cccaatcatt tgagaagtta aaacctacac atgaaggtag
      901 gtattctaga agattgaata gacaacaccg ggttgtttat aaggtagatg aagatgaaca
      961 cgtggttgaa atttattcag caaggacgca ttacgagtca cagtaattac ttgtaaatac
     1021 attatctcgt ggtataatgt atttactaag taattacata ctgttagaaa tgagggattt
     1081 aaatgattac aaaaacaaga aaacaaggca attcaatcat gttgacagta cctaaagatt
     1141 ttaatgttcc taatggagtc gaagttgaag caaagttggt agagaatggt attctttatg
     1201 aatttgttga accacaaaaa gaattttatg attttagcga agatattttg tctgatataa
     1261 ttgcagaagg ctacgataaa gatgagattc ttgtagaatt taagaataga aaaaacaaaa
     1321 tgcattcttc ctttagagat attgctgaag atacacttac taattctaaa gtaatgacta
     1381 aagaggagtt ggcaacagaa attggcttat gaaatcgttc cttcaaaac
//
LOCUS       DADOZM010000262         1420 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.270, whole genome shotgun sequence.
ACCESSION   DADOZM010000262 DADOZM010000000
VERSION     DADOZM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.270"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(150..1337)
                     /locus_tag="J8I47_002891"
     CDS             complement(150..1337)
                     /locus_tag="J8I47_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAZ6324281.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          309 c          249 g          449 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact
//
LOCUS       DADOZM010000263         1374 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.271, whole genome shotgun sequence.
ACCESSION   DADOZM010000263 DADOZM010000000
VERSION     DADOZM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.271"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            33..1372
                     /locus_tag="J8I47_002892"
     CDS             join(33..474,474..1372)
                     /locus_tag="J8I47_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAZ6324282.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DADOZM010000264         1373 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.272, whole genome shotgun sequence.
ACCESSION   DADOZM010000264 DADOZM010000000
VERSION     DADOZM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.272"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            191..445
                     /locus_tag="J8I47_002893"
     CDS             191..445
                     /locus_tag="J8I47_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ6324283.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="J8I47_002894"
     CDS             446..763
                     /locus_tag="J8I47_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ6324284.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DADOZM010000265         1343 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.274, whole genome shotgun sequence.
ACCESSION   DADOZM010000265 DADOZM010000000
VERSION     DADOZM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.274"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(305..661)
                     /locus_tag="J8I47_002895"
     CDS             complement(305..661)
                     /locus_tag="J8I47_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAZ6324285.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="J8I47_002896"
     CDS             complement(675..1085)
                     /locus_tag="J8I47_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ6324286.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          434 a          246 c          187 g          476 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agc
//
LOCUS       DADOZM010000266         1286 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.275, whole genome shotgun sequence.
ACCESSION   DADOZM010000266 DADOZM010000000
VERSION     DADOZM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.275"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            4..>1286
                     /locus_tag="J8I47_002897"
     CDS             4..>1286
                     /locus_tag="J8I47_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324287.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKG"
BASE COUNT          531 a          204 c          285 g          266 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc
      301 ctggacatga cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaagg
//
LOCUS       DADOZM010000267         1269 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.276, whole genome shotgun sequence.
ACCESSION   DADOZM010000267 DADOZM010000000
VERSION     DADOZM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.276"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            22..1184
                     /locus_tag="J8I47_002898"
     CDS             join(22..298,298..1184)
                     /locus_tag="J8I47_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ6324288.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          457 a          227 c          253 g          332 t
ORIGIN      
        1 aacataccag gaggaatttt tatgtctaag agaacacgaa gaactttttc acaagaattc
       61 aagcaacaaa tcgtcaatct ttacttagct ggaaagccac gtgtagaaat cattcgagaa
      121 tatgaactaa cagcttcagc atttgacaaa tgggtaaagc aatctaaaac gagtggttca
      181 ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact acgtaaaaga
      241 aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat attcggacga
      301 agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg tgcagaatat
      361 taggtctatc acgtcagtcc tattattatc aatcaaaacc aaagaaagac gaatcagaac
      421 ttgaagaagt agtcgctgaa gaatttatcc gcagccgaaa ggcctacggc tcaagaaaaa
      481 taaaaaaagc cttatcaaaa cgaggcattc agatcagccg acgaaaaatt agtagaatca
      541 tgaaaaatag aggattaaaa tcgagctata ctgttgctta ttttaaagta catcattcta
      601 cttgcaatga agccaaaacg acaaacgtat tgaatcgtaa attcttaaga gacaacccat
      661 tagaagcgat cgtaacagac ttgacttatg tacgagtcgg gaaaaaatgg aattatgtct
      721 gtttcatttt ggatctgttc aatcgagaaa ttctcggcta ttcttgtgga gaacataaag
      781 atgccgttct agtaaaaaaa gcatttagcc gtatcaaaca acctctgaca gaggttgaga
      841 tttttcatac tgatcgtgga aaagagtttg ataaccaagc tattgatgaa ttattaacaa
      901 cttttgacat caatcgatca ttgagtcata aaggctgtcc ttttgataat gccgtagctg
      961 aatcaactta taagtcgttg aaagtagaat ttgtctatca atacacattt gaaaccttac
     1021 aacaattgga tttggagtta tttgactatg tcaattggtg gaaccacctt cggttgcacg
     1081 gtacacttgg ctacgagaca ccggttggtt accgtaacca gagattggcg cagcgaatcc
     1141 ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa ctgttagctc
     1201 ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga atagcatacg
     1261 gaaagaagt
//
LOCUS       DADOZM010000268         1268 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.277, whole genome shotgun sequence.
ACCESSION   DADOZM010000268 DADOZM010000000
VERSION     DADOZM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.277"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            7..624
                     /locus_tag="J8I47_002899"
     CDS             7..624
                     /locus_tag="J8I47_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324289.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          478 a          163 c          201 g          426 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataa
//
LOCUS       DADOZM010000269         1250 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.278, whole genome shotgun sequence.
ACCESSION   DADOZM010000269 DADOZM010000000
VERSION     DADOZM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.278"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..915)
                     /locus_tag="J8I47_002900"
     CDS             complement(<1..915)
                     /locus_tag="J8I47_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324290.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYF"
BASE COUNT          408 a          223 c          124 g          495 t
ORIGIN      
        1 aaaatattgt tcaattattt ctttatatat tttctcatag tatgtttctg tccatcctaa
       61 tgttcctata aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt
      121 aataatgttg tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc
      181 aatttcctcc caataatatg aatctctttc aattcccatg atggtagctt tggctaattt
      241 caaatcaaat aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc
      301 tttaatccat tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt
      361 atatttcaaa tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct
      421 tttaagtact tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt
      481 attcactaat aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg
      541 aaaatcacct ttataaactc cattcttttc cactgtttta ttaatatttt ttaaaaacca
      601 gaatgagttt gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg
      661 taataataag acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc
      721 aatcataggg agccattttt tgattttcac taattgattg ctcataaatc tttcactgga
      781 cttttgttca atcttaaatt gcaaaacttc taactcataa ttcccatctt ctgatttatt
      841 tttgggaaca actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc
      901 ataatttgta gccattaata catctcctta ttcataataa atttcatatg acgctcctct
      961 caaaatttac cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct
     1021 taaaaaaaaa ctggtctctc taccggtaat atcgattgag tccgccttta tcgactttat
     1081 ccttttttta tattaaattt tttcaccggt agacagggag agaaagaata aactaaaaag
     1141 cccttaattc ttaatgtttt cacctcctta ccgttcctac cgctaaaatt taatataact
     1201 aaattctata acattagaag aaattttaaa tacctttgat aaaaaatatt
//
LOCUS       DADOZM010000270         1246 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.279, whole genome shotgun sequence.
ACCESSION   DADOZM010000270 DADOZM010000000
VERSION     DADOZM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.279"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            200..742
                     /locus_tag="J8I47_002901"
     CDS             200..742
                     /locus_tag="J8I47_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324291.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="J8I47_002902"
     CDS             832..>1246
                     /locus_tag="J8I47_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAZ6324292.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DADOZM010000271         1245 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.280, whole genome shotgun sequence.
ACCESSION   DADOZM010000271 DADOZM010000000
VERSION     DADOZM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.280"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1244
                     /locus_tag="J8I47_002903"
     CDS             <1..1244
                     /locus_tag="J8I47_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAZ6324293.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DADOZM010000272         1222 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.281, whole genome shotgun sequence.
ACCESSION   DADOZM010000272 DADOZM010000000
VERSION     DADOZM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.281"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            113..>1222
                     /locus_tag="J8I47_002904"
     CDS             113..>1222
                     /locus_tag="J8I47_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ6324294.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFI"
BASE COUNT          410 a          156 c          160 g          496 t
ORIGIN      
        1 taaaaaatat tttattaata ataaaattaa tcacatttta tatagtgagt aatctagtgt
       61 tttaaaaaaa taaatgatac tatatgtaat ataaaaaaac atgaggtggt atatgaattt
      121 gaaaaaaata ttttctgtaa aaatatttat agtttatgca tttgttagtt tattatattt
      181 atttccagca tttaaaaaca tggaaatatt tggcttttat gatacggcat ttcacattaa
      241 tcgtgcgtta agtttagaat ctatttttag ctcccctatt aattttgaaa cgtttagatc
      301 atatggcatg caagtaaata atttttaccc atggttaact ctatatcctt tatttttatt
      361 gatcaagttt actaatttag caataggcta taaccttttt ctttatattg ttacgctcat
      421 tactctattt atatgtcatt atgtaatgta tgaaattacc aaaaaacatg ttactagctc
      481 ctttttcgct attatatata caacatcttc ttttaggagt gtagaaatat ttcttcgagg
      541 ggcaatgggg gaattactcg caatgtcaat acttcctctt attttactcg ggtttataaa
      601 gctttacgac tcaaaaaaag aatcttgggt tatgttggcg attagcatga ctttattaat
      661 ttacactcat gttctttctg tagctatgac tatggtaatg attataattg ctttaattat
      721 tcaattttat cggaaaaaga aaatagaaat cttcttaatt attaaattaa taaaagctgc
      781 tatagttact ttacttcttt cactagcgtt tataggtcct atgattgagc agacattgta
      841 tcaagatctg aatagacctt atgtggatat attacaaaaa agagcaattt atctgggtaa
      901 agagtttttg attggaagta tagattcaga attactatca tttggtattg gcttaacttt
      961 gcttatttgt ttaattatac taacatttaa ctttaaaaaa ttaggaatct tatctaaaat
     1021 cacttatata atgggtataa tatctattct actttgtaca aaaatatttc catggtttgc
     1081 cttacaaaat actcctttaa atattattca agctccttgg cgttttatga tattttcaac
     1141 attgttcata tctttttcag tatctctatt tatttctcca cagttggaaa aattttctta
     1201 tacaaggaga agaaatttta ta
//
LOCUS       DADOZM010000273         1203 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.282, whole genome shotgun sequence.
ACCESSION   DADOZM010000273 DADOZM010000000
VERSION     DADOZM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.282"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..94
                     /locus_tag="J8I47_002905"
     CDS             <1..94
                     /locus_tag="J8I47_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAZ6324295.1"
                     /translation="EDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            99..1046
                     /locus_tag="J8I47_002906"
     CDS             99..1046
                     /locus_tag="J8I47_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAZ6324296.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          394 a          176 c          258 g          375 t
ORIGIN      
        1 agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct
       61 tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg
      121 gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa
      181 tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca
      241 cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata
      301 aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac
      361 cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca
      421 agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg
      481 aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg
      541 ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta
      601 gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg
      661 gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat
      721 ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc
      781 aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa
      841 cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag
      901 aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata
      961 ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta
     1021 attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag
     1081 gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata gaagggctct
     1141 ttgtcaaatg atgttggtaa agagaaatct tagaataatt tggacagaac agaaagaaga
     1201 aaa
//
LOCUS       DADOZM010000274         1203 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.283, whole genome shotgun sequence.
ACCESSION   DADOZM010000274 DADOZM010000000
VERSION     DADOZM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.283"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..336)
                     /locus_tag="J8I47_002907"
     CDS             complement(<1..336)
                     /locus_tag="J8I47_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324297.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLT"
     gene            complement(329..562)
                     /locus_tag="J8I47_002908"
     CDS             complement(329..562)
                     /locus_tag="J8I47_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324298.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(559..>1203)
                     /locus_tag="J8I47_002909"
     CDS             complement(559..>1203)
                     /locus_tag="J8I47_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAZ6324299.1"
                     /translation="FNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFI
                     TQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTW
                     WRLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLA
                     KNTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          350 a          265 c          168 g          420 t
ORIGIN      
        1 ggtcaatttt tgttgatcac gaatcatttc tgttaattca gaaatatgtt tgtccttctc
       61 agctaattga tcacgcaaaa actcattctc tttttctaaa ggaaaatcac tgcctgtgtt
      121 attgaccggt aattgaccag tcaattgatc actttttgac cgataaattg accgaacaac
      181 accaatcaac tctttatcta gcatgataat gccattttct ttatactgag caaggtcaaa
      241 ccctgaattg accagttgat tgcgcactgt atttttcgat acacccaatt cgtcagcaag
      301 attttttatg gtcatctttt ctgacaaatc agtcattagc ttctccacta ctttcagcta
      361 ccgcataaat atatttaacg gcgtgcttag ggataataat cggagagtca attttattat
      421 ttttcaaaat caattcatat tgcgttacat caagtaactt accggacatt tttttgctat
      481 ccaaaaaaac aaccacaacg taatcacctg ttaaatttac caaccaattt ttcttaccaa
      541 aatacttttg tttttctgtc atttttcttc ctcctctgtt acgtctagcc ctagaagcca
      601 tgtgtctaat tcttttttta aatcgtctgc cgattccgca aaggtttctc gcaaatgatt
      661 cgtcaattca tcattttgac tttcttgctt caacaaatcc ctgtattttt tctttgtatt
      721 cttagctaga tttccccact cagaatgatc ataaagcaaa cctttcacca taatccgttc
      781 agtaacttta gtatttggcg gaaaataatg tggactagca aagctatcca aactttcatg
      841 aaccatctca tgccaatcag ttgcttttcc ccttcgtaac tgcaattcta gtcgccacca
      901 tgtcgttatt tccggcggaa caattttacg ttttctttct tgttccagtt ttttattata
      961 catccgaatt tgtcgttcac tggcacgact accccaatat gccgtttcta acttaccaga
     1021 acgcccataa attggtttaa aagacacagg atcaactacc cgatattgtg taataaactc
     1081 atcaggtaca tcgataatgt cgcaagcaat atccgcacgt gaaaagtgag gttcgataaa
     1141 caaatcatga ataaaatttt tcatggaact agctaagaat tgactaatct tattcggatt
     1201 gaa
//
LOCUS       DADOZM010000275         1195 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.284, whole genome shotgun sequence.
ACCESSION   DADOZM010000275 DADOZM010000000
VERSION     DADOZM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.284"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            187..888
                     /locus_tag="J8I47_002910"
     CDS             187..888
                     /locus_tag="J8I47_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ6324300.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          428 a          209 c          195 g          363 t
ORIGIN      
        1 gttttttata cacaaatcat tcgcttcaaa acaaattatt caataccatt gtatattttt
       61 tacataataa caaattatat ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta
      121 aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac
      181 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
      241 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      301 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      361 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      421 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      481 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      541 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      601 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      661 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      721 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      781 actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag
      841 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      901 taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga
      961 aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt
     1021 tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc
     1081 ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa
     1141 tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca ataat
//
LOCUS       DADOZM010000276         1190 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.286, whole genome shotgun sequence.
ACCESSION   DADOZM010000276 DADOZM010000000
VERSION     DADOZM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.286"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            173..>1190
                     /locus_tag="J8I47_002911"
     CDS             173..>1190
                     /locus_tag="J8I47_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ6324301.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKII"
BASE COUNT          485 a          110 c          233 g          362 t
ORIGIN      
        1 aaatttaaat aatatatatg atgaaaaagt atggaactta ttaaagaaag atactttact
       61 gtttaagttg acatggaaac aatcttttaa aaagcaaaaa gatgataaag ttacttttta
      121 tgggatgttg gtagaaaaca aattattata aatattagag gtataaaaag ttatgaaaca
      181 caaggtaagt gtgatagtac caatttacaa tgttgaaaaa tatttaagaa aatgtgtgga
      241 tagtttattg agtcagacat taaaagagat agaaatcgta ttagttgatg atggttcgcc
      301 agatgcttcg ggggaaatag cagatgagta tcaaaaaaaa tattctaatg taaaaacaat
      361 acatagagaa aatggggggt taggaccggc aagaaataca ggaattgaga atgcgacagg
      421 agaatatgtg gcttttcttg attcagatga ttgggtagaa agtgatatgt atgaaaaatt
      481 atacttagtt gcgtccaaag aaaatgctga tatagtagtt agtggacatt gtgattttgc
      541 gaatgacaaa gctatggtga aaaagcaaca tcctttgagt gggaatgtat atgatacgcc
      601 taatagtatt aaaaatgtac gaaaaaattt atttggacat agtccaaaag atcaagaagt
      661 agaggccttc ccgatgtcag tatgtatgtc tttgtataat aaaaatctgc tagataaata
      721 tagactaaga tttgaaaaaa ttttatctga agatacaatt tttaacttga atgcatatga
      781 tgtcgcaaat aaaattgctt ttacagaata tacagattat tgttatagaa aagaagaaca
      841 agagtctatt acaaaaagtt tatctccaaa taagcagacg gaatttaaag aatttttaat
      901 aactctaaaa gctatagcat cgaaagaaga tttagaatgt caaatcagag caaaacgtat
      961 ggcaattgat tattgtaggc tttatgtggg cttagttgat gatgcaaatt tgggaattaa
     1021 agagaaaaga aagtatataa aaaaatttat tgaagatgag ggattaattc aatgttggaa
     1081 gggatatcct acaaatacgc taccatttca acaaaaattt tttcataaaa tgcttgaatg
     1141 taagtgttat acaatattgt tgttgttaag tagaattaga aagataatta
//
LOCUS       DADOZM010000277         1186 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.287, whole genome shotgun sequence.
ACCESSION   DADOZM010000277 DADOZM010000000
VERSION     DADOZM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.287"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(17..90)
                     /locus_tag="J8I47_002912"
     tRNA            complement(17..90)
                     /locus_tag="J8I47_002912"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Met,seq:cat)
     gene            complement(103..192)
                     /locus_tag="J8I47_002913"
     tRNA            complement(103..192)
                     /locus_tag="J8I47_002913"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Ser,seq:tga)
     gene            complement(216..289)
                     /locus_tag="J8I47_002914"
     tRNA            complement(216..289)
                     /locus_tag="J8I47_002914"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Ile,seq:cat)
     gene            complement(303..376)
                     /locus_tag="J8I47_002915"
     tRNA            complement(303..376)
                     /locus_tag="J8I47_002915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(340..342),aa:Met,seq:cat)
     gene            complement(404..476)
                     /locus_tag="J8I47_002916"
     tRNA            complement(404..476)
                     /locus_tag="J8I47_002916"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Ala,seq:tgc)
     gene            complement(496..569)
                     /locus_tag="J8I47_002917"
     tRNA            complement(496..569)
                     /locus_tag="J8I47_002917"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(533..535),aa:Pro,seq:tgg)
     gene            complement(579..652)
                     /locus_tag="J8I47_002918"
     tRNA            complement(579..652)
                     /locus_tag="J8I47_002918"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Arg,seq:acg)
     gene            complement(663..748)
                     /locus_tag="J8I47_002919"
     tRNA            complement(663..748)
                     /locus_tag="J8I47_002919"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Leu,seq:taa)
     gene            complement(765..836)
                     /locus_tag="J8I47_002920"
     tRNA            complement(765..836)
                     /locus_tag="J8I47_002920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Gly,seq:gcc)
     gene            complement(848..920)
                     /locus_tag="J8I47_002921"
     tRNA            complement(848..920)
                     /locus_tag="J8I47_002921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Thr,seq:tgt)
     gene            complement(938..1019)
                     /locus_tag="J8I47_002922"
     tRNA            complement(938..1019)
                     /locus_tag="J8I47_002922"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(983..985),aa:Leu,seq:tag)
     gene            complement(1027..1099)
                     /locus_tag="J8I47_002923"
     tRNA            complement(1027..1099)
                     /locus_tag="J8I47_002923"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1064..1066),aa:Lys,seq:ttt)
     gene            complement(1103..1175)
                     /locus_tag="J8I47_002924"
     tRNA            complement(1103..1175)
                     /locus_tag="J8I47_002924"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Val,seq:tac)
BASE COUNT          306 a          332 c          282 g          266 t
ORIGIN      
        1 tgtttttagt ttttaattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc
       61 cgacgagcta ccagactgct ccatcccgcg ataatatgaa aaaggaggat aagggattcg
      121 aacccttgca cggttttacc cgcctgacgg ttttcaagac cgttcccttc agccggactt
      181 gggtaatcct ccatatgaaa cttacaacgc cacaatggac cttgtaggac tcgaacctac
      241 gaccggacgg ttatgagccg tctgctctaa ccaactgagc taaaggtcca ggctcttaaa
      301 aatcgcggcg gaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca
      361 gctgagctac accgcgaaaa agatattcat tatttaaatg aaatggagcc tagcgggatc
      421 gaaccgctga cctcctgcgt gcaaagcagg cgctctccca gctgagctaa ggccccgtgt
      481 ttaaatttat aattatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca
      541 agtgctctac caagctgagc tacttcccgt ttaattaacg cgcccaagag gagtcgaacc
      601 cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc gcatatttga
      661 aatgccgagg accggaatcg aaccggtacg gtgatcactc accgcaggat tttaagtcct
      721 gtgcgtctgc cagttccgcc accccggcgc gattgctttg gcaaagcgga aaacggggtt
      781 cgaacccgcg acccccacct tggcaaggtg gtgctctacc actgagctat ttccgcgtgt
      841 ttaaagatgc cggctaaagg acttgaaccc tcgaccctct gattacaaat cagatgctct
      901 accaactgag ctaagccggc atgtaaaact taaattatgc gggtgaaggg acttgaaccc
      961 ccacgccgta aggcgctaga tcctaaatct agtgcgtctg ccaattccgc cacacccgca
     1021 aaaatatgag ccgtacaggg ctcgaacctg tgaccctctg attaaaagtc agatgctcta
     1081 ccaactgagc taacggctca tatggaggtt aacgggatcg aaccgctgac cctctgcttg
     1141 taaggcagat gctctcccag ctgagctaaa cctccatgga taactt
//
LOCUS       DADOZM010000278         1171 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.288, whole genome shotgun sequence.
ACCESSION   DADOZM010000278 DADOZM010000000
VERSION     DADOZM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.288"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..102)
                     /locus_tag="J8I47_002925"
     CDS             complement(<1..102)
                     /locus_tag="J8I47_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAZ6324302.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQD"
     gene            complement(113..1135)
                     /locus_tag="J8I47_002926"
     CDS             complement(113..1135)
                     /locus_tag="J8I47_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAZ6324303.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          307 a          255 c          185 g          424 t
ORIGIN      
        1 atcttggatc tcaaaatacc catcttttcg ggtagcttta gaaaagctat cggcataagc
       61 cttcgcttgt gacggttttt ctgctaaaat aacggttttc atcatgacga tttcactccc
      121 tttcttgctg tctaccttgt tgaaacaaca tggcacgttt ttgctttctg atctcttgtt
      181 ggtagatttc atgcttcaat tgatcattcc aatccttttt tacttcaggt acaaattcat
      241 gttcatgatt tttatagcct ttgtagtatt gttctgcttt ataagagaaa agaatctgag
      301 cattaatatc ccctttttct tgataaaaac gatcaaaatc gtacgtacca tccacgcgtt
      361 cacaagcttt taattctttt gcgacttctc tacaaatatc tttcaaatta atagcttcta
      421 ttgaatcgtt aacagaaagc tgctgaccaa aagacgcatt tactttgtga gcggaaacca
      481 attggttcgt taattgcaga ttgttttctg tcttgtgtat ggccataata gcttctggtg
      541 tgatcttttc ttcttttgcg acttcttcat aaatgacttt gtattctttt ggcacttgcc
      601 aatcattagc gattcctcgt tcacaacgta ctttctgatt cgtaaatgga tcaactgcgc
      661 ccattaattg ttctttttcg tagaaaatat gtcctgcacg gtcattatcc acacaaatct
      721 cgatggattg aggaaaggct tgtttctttc tcaattcaga tactgcctca ccaaaataat
      781 gactgatcac atggtgtttt aacccttcca tcgaaactaa ccacgtatcg tttaactgat
      841 cacgatttag tgcagcatag ctaagtaaat caatactact ctcaaaaaat tttaactgct
      901 tagggtcacc gatctttagg ttaaagccat gattcgctgt cgaattttta tctatatgct
      961 tatatgtgcc tctctcacca aaacgttcat aatcaatgaa cgttccttga taactcgccc
     1021 ctactgtctc atttgtctgt ggatctttcc ataaaaaggc gaccgcatga tctaaacgat
     1081 cgtctcgaac taagaaattt tgccgttcat gttttggctt tagttcggca atcaaccctt
     1141 ctttttctgc ataatcaatc agccactctg g
//
LOCUS       DADOZM010000279         1115 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.289, whole genome shotgun sequence.
ACCESSION   DADOZM010000279 DADOZM010000000
VERSION     DADOZM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.289"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="J8I47_002927"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="J8I47_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAZ6324304.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DADOZM010000280         1092 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.290, whole genome shotgun sequence.
ACCESSION   DADOZM010000280 DADOZM010000000
VERSION     DADOZM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.290"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            149..>1092
                     /locus_tag="J8I47_002928"
     CDS             149..>1092
                     /locus_tag="J8I47_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAZ6324305.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAF"
BASE COUNT          395 a          193 c          194 g          310 t
ORIGIN      
        1 tggaattttc ataaaatgtt tcaatgaagc gcgacaacgt tgtttaatat atcgcgaatt
       61 gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg tatttccgac
      121 gaaagaaaac aaaggagtgt aaccataaat gacctacaaa catcttacca tagacgaact
      181 gacaatgata gaatcatatt atcttcaaca taataagccg gttgaaatcg ctaaccgaat
      241 gggacgtgct atacaaacta tttataatgt agtcaataag ttcaagcaag gcaagactgc
      301 tcttgattat tggcaccagt ataaagaaaa taagaaaaaa tgtggtagaa aagtcattca
      361 attacctgct catgaagtag attacattaa agagaaagtc actcttggtt ggacgcctga
      421 cgtcattatc gggcgaaaag aaaggcctgt ttcatgcggt atgagaacac tttatcgttt
      481 attttctaaa ggaatatttg atattgacac actaccgatg aaaggtaaaa gaaaacccaa
      541 tggccatcag gaaaaacggg gaaaacaaca gtatcagcgc tcaatccatg atcgacctga
      601 taattatcct gatttcaatt ctgagtttgg tcaccttgaa ggtgatacga tcgttggcat
      661 tcatcataaa agtgccgtca ttactttagt tgaaagatta tctaaagtca ttatcacgat
      721 taaacccaac ggccgtaagg cattagatat tgaaactgcc cttaatcaat ggttttctcg
      781 cttccctaaa aacttcttta aatctattac gtttgactgt ggaaaagaat tttctaactg
      841 gaaagccatt agtaaccaac atgatattga tatatatttt gcggaccctg gaacaccttc
      901 tcaacgccca ttaaacgaga attctaacgg gattctgcgt cgtaatggac tgccgaaatc
      961 aatggatttt agaaaagtga atcagacatt tatttccagt gtcagcaatc aacgtaatca
     1021 tattccaaga aaatcattga attacagaac accaatcgaa atatttttga gctatgtaca
     1081 agaagcattt ta
//
LOCUS       DADOZM010000281         1089 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.291, whole genome shotgun sequence.
ACCESSION   DADOZM010000281 DADOZM010000000
VERSION     DADOZM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.291"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            414..947
                     /locus_tag="J8I47_002929"
     CDS             414..947
                     /locus_tag="J8I47_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAZ6324306.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          350 a          203 c          200 g          336 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgttta
//
LOCUS       DADOZM010000282         1087 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.292, whole genome shotgun sequence.
ACCESSION   DADOZM010000282 DADOZM010000000
VERSION     DADOZM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.292"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            64..849
                     /locus_tag="J8I47_002930"
     CDS             64..849
                     /locus_tag="J8I47_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAZ6324307.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          427 a          161 c          177 g          322 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
       61 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
      121 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
      181 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
      241 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
      301 aataaataca tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc
      361 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
      421 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
      481 agtattttag aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc
      541 ggctctgtat atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa
      601 gtgaaaaata aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca
      661 attagaaaat ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc
      721 cccaattgga aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta
      781 actgaaaata gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc
      841 gatgactaac aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa
      901 acaaaaaaac tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg
      961 gatttacgaa acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat
     1021 tactaaactt aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt
     1081 ttaaaag
//
LOCUS       DADOZM010000283         1083 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.293, whole genome shotgun sequence.
ACCESSION   DADOZM010000283 DADOZM010000000
VERSION     DADOZM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.293"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            1..492
                     /locus_tag="J8I47_002931"
                     /pseudo
     CDS             1..492
                     /locus_tag="J8I47_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="J8I47_002932"
                     /pseudo
     CDS             712..1008
                     /locus_tag="J8I47_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          387 a          204 c          224 g          268 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tga
//
LOCUS       DADOZM010000284         1078 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.294, whole genome shotgun sequence.
ACCESSION   DADOZM010000284 DADOZM010000000
VERSION     DADOZM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.294"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..987
                     /locus_tag="J8I47_002933"
     CDS             <1..987
                     /locus_tag="J8I47_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324308.1"
                     /translation="SNAEFENFIKLCSTLLYLILAYGYACTAFYLFKAEGIDFIFWSG
                     VISYMSGSVFYLIVSYGLNGLISYIKSLITGETNTANFAMEVHDLTFAMGVFFLYYVF
                     FENKNTKKHKIKIIVSLILIFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTI
                     CATVISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWF
                     SKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKF
                     GLKTGECVLLLTIYMFILYLTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
BASE COUNT          350 a          106 c          194 g          428 t
ORIGIN      
        1 tcaaatgcag aatttgaaaa ttttataaag ttatgttcca cgttgcttta tttaattctt
       61 gcatatggtt acgcatgtac agcattttac ttatttaagg cagaaggaat tgattttatt
      121 ttttggtcag gtgttatttc atatatgagt ggttcagttt tttatttaat tgtatcttat
      181 ggtttaaatg gtttaatttc ttatataaaa agtcttataa caggtgaaac aaatactgca
      241 aattttgcta tggaggtaca tgatttaact tttgcgatgg gggttttttt cctttattac
      301 gtattttttg aaaataaaaa tacaaaaaaa cataagataa aaattatcgt atctttaatt
      361 ttaattttca tgggattgaa gagaattgaa ctgttagctt taagtggtgc aattctagtg
      421 tattatgttt tattaaagtg gggaaaaaag attcaaatcc gtgcattagt gtgtactatt
      481 tgtgcaactg ttatttcgtt agtgtttatt tacattatag atacaggaat tttggcattg
      541 cttatgtcaa atttgggcat tacagatgac ggtgcgaggc tttcatttta tctatattca
      601 tctaaatttt atgaacttgg tttaggatat gtaggaacag gatggacatg gttttcaaaa
      661 tactttttta atctttattt atcgcacttt aggttaaatg gtggaagaat tgcagcaagc
      721 ctacacagtg atatattaac tatttatata gaagttggat gtatagtttt tattatatgg
      781 atgttttggt tattttttgt gaaaccgata agactaacta aaaaatttgg attgaaaaca
      841 ggggagtgtg tattattatt aactatatat atgtttatat tatatttgac ggataatacg
      901 ctcacttatc cagacactca aatgattttt ctcttagtac ctatgatagt ttcgtgggtt
      961 gataatcaac aacgaatatc ggaatagata gctaaagtat attttaatta tgaatttata
     1021 attagttgta aaagttaata tttaacggac aggaagtagg gtgaaaaaat ttgaaaaa
//
LOCUS       DADOZM010000285         1075 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.295, whole genome shotgun sequence.
ACCESSION   DADOZM010000285 DADOZM010000000
VERSION     DADOZM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.295"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="J8I47_002934"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="J8I47_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAZ6324309.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          317 a          221 c          182 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaa
//
LOCUS       DADOZM010000286         1062 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.296, whole genome shotgun sequence.
ACCESSION   DADOZM010000286 DADOZM010000000
VERSION     DADOZM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.296"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..993)
                     /locus_tag="J8I47_002935"
     CDS             complement(37..993)
                     /locus_tag="J8I47_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324310.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DADOZM010000287         1052 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.297, whole genome shotgun sequence.
ACCESSION   DADOZM010000287 DADOZM010000000
VERSION     DADOZM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.297"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            6..980
                     /locus_tag="J8I47_002936"
     CDS             6..980
                     /locus_tag="J8I47_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAZ6324311.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          361 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt tt
//
LOCUS       DADOZM010000288         1052 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.298, whole genome shotgun sequence.
ACCESSION   DADOZM010000288 DADOZM010000000
VERSION     DADOZM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.298"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            47..739
                     /locus_tag="J8I47_002937"
     CDS             47..739
                     /locus_tag="J8I47_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAZ6324312.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          462 a          119 c          156 g          315 t
ORIGIN      
        1 tttttaataa cttatttaag gaaatttttt gaaggagaag ataaaagtga gaaaaaagat
       61 tattattaat gctggagtaa ttataggatt gatgttagta attttctccg gaaagcaata
      121 tgctgataat attttattat ggggagggga agataatatt gaaaagatca acaacaattt
      181 aactatatta gacacagctc tagaaaataa agagcaaaaa attagtattt taaattcaca
      241 attgtcttca aacaaccaaa ctcttgaaca actaagaact aatattgaag tttccaaaca
      301 aaaagtggca tctttagaaa acgacaagaa tcaattagta tcagagaaaa ctaatttaga
      361 aaatcaattg tcagcaaaag actctgaatt gcaaggaaaa caaaatgaaa ttaatgcgaa
      421 aatagacgaa attaaccaaa aagtagctga aataaatcga ttaaatagcg attggaataa
      481 tcgtttacaa aatgaaaaga acaaatttaa tgaagaacaa tctactatta atagtttaaa
      541 ccagcaaatt aatcaaatgt tgagtgaaaa aaataacatt caaaatcaac tgcaagctac
      601 tcagcaaaag ctagaagaag aaaagtcaaa aaatagtagt ttgaataatt acattgaaaa
      661 gcttcaaaaa gctaaaaatg atgtagagga taccgcaaat aaagcacaac aaattgtaaa
      721 tgaacataca atccaataga tatagtttta atttaacaaa tggtatgttt ggagataatg
      781 agatagaata taaatgtcat aatattatac aatctaaaca ccgtttcctt ttctattttt
      841 gatgacatca gtatatagct actcgaaaga gtagtctttt tattattctc gcttattatt
      901 gatatgaaaa tttcgatttg acaaaaggta ccgaataatt ttgtaatatg aattattatc
      961 aagaagtaat tatgatgata acatatggaa actagttagt aataataaca ggtgattaaa
     1021 cttttgaacg acttgtaatt ctatattttt tc
//
LOCUS       DADOZM010000289         1043 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.299, whole genome shotgun sequence.
ACCESSION   DADOZM010000289 DADOZM010000000
VERSION     DADOZM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.299"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(61..279)
                     /locus_tag="J8I47_002938"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="J8I47_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="J8I47_002939"
     CDS             complement(545..739)
                     /locus_tag="J8I47_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324313.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          361 a          180 c          153 g          349 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aat
//
LOCUS       DADOZM010000290         1037 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.300, whole genome shotgun sequence.
ACCESSION   DADOZM010000290 DADOZM010000000
VERSION     DADOZM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.300"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            59..>1037
                     /locus_tag="J8I47_002940"
     CDS             59..>1037
                     /locus_tag="J8I47_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAZ6324314.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEF"
BASE COUNT          350 a          196 c          204 g          287 t
ORIGIN      
        1 cgttctctag tatgattaaa tcaccacaaa ataaacaact gagaggacga tacgctatat
       61 gaatgattct atcaaaaaaa tgctgagaat aacagagaaa gatttgatga ttacagaggt
      121 ctcttacgag acccttcaga agaaaaagac gttggtcgtc gatgctgttc tctcgcctac
      181 tcctcgtgct tgtagaagtt gtggttctac tgtggtagat ggaaacggga aagcaattat
      241 agtgaaaaat ggaaaaaagg aaacgattgt ccgttttgaa caatacaatc atatgccttt
      301 ggttatgcgc ctaaaaaagc aacgctatac ctgtaaaaac tgtcgcaccc attggacggc
      361 tcaaagttat tttgtccaac ccagacattc aatcgcaaat catgttagat ataaaattgc
      421 ttctttactg actgaaaaag tatctttatc ttttattgcg aaaagctgtc aggtatcttt
      481 gaccaccgtt attcgtacat tgaaagagtt taaaagctat ttaccaaagc aatctaagaa
      541 gatcctcccc agagtattga tggttgacga atttcgttcg catgcttcca tagaagataa
      601 gatgagcttt atttgcgcgg atggcgaaac aggaaaatta atagatgttt tgcctacgcg
      661 taaattacct cgattaacaa gctatttctt aggttgtacc aatccagaag aagtagaatt
      721 cttggtgaca gacatgaacg ccgcctactt ccagctcacc aaacgtgttc tgccaaatgc
      781 gaaagtcgtg attgatcggt ttcatattgt caaacacatg aatcaagcgt tcaatgagtt
      841 gcgtatccgt gaaatgaatg aacttcgtaa agccggacag aaaagccagg cagaaaaact
      901 gaaaaagaac tggcgctttt tgctaaaaaa tcgtgcaaac atcaaccatt atgaatacaa
      961 aacatggaaa agtttccgag caccaaaata cccatttctt actgaagcaa tgatgattga
     1021 tcgattgctt gagtttt
//
LOCUS       DADOZM010000291         1027 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.301, whole genome shotgun sequence.
ACCESSION   DADOZM010000291 DADOZM010000000
VERSION     DADOZM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.301"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..345
                     /locus_tag="J8I47_002941"
     CDS             <1..345
                     /locus_tag="J8I47_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAZ6324315.1"
                     /translation="GNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEK
                     VPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILES
                     KYYELFELIQRG"
     gene            351..>1027
                     /locus_tag="J8I47_002942"
     CDS             351..>1027
                     /locus_tag="J8I47_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ6324316.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLA"
BASE COUNT          397 a          132 c          191 g          307 t
ORIGIN      
        1 ggaaatagaa atgatataga aaaattttat caagcatttg acgtattaat gttgccatca
       61 ttatttgaag gggttccgtt agtgggtatt gaagctcaat ttgcagattt accgtgtttc
      121 ttttcggaga aagttcctac agaagttgct ttcagtgata agaccaattt cataagttta
      181 aatcaatcgg caaaagaatg ggcgagagag attgcaaatg tagatatctc tcatcgagat
      241 tcagaacgtt cgattttaat aaaggcagat tataatattc aaacagcata caaaatctta
      301 gaatcaaaat attatgagtt atttgaatta atacaaagag gttaaagaat atgacacaga
      361 aacattcaag tataaaaaga attatacatc gcatatccga agaattgaat gcaacaagac
      421 gaattgcaaa gataacatct tggaaggaag catttgccac gttacaagct aaaattgata
      481 ttcaagtcat gaatcgtaat ggatataatg aaagtaaaaa acagaaggaa catttattaa
      541 aaaaacatga ggtaatgctg aagtattatg aaaagacttt ttcagatttt ttgaaaacat
      601 atgattatag tcaaaatcaa ataattaatc aatcaaaatc accatattct aactgcattt
      661 gggtttgttg gtggcaggga ttggatcaat cgccggaaat tgtaaaagct tgtgtaaaat
      721 caataaaacg aaatgcaggg aaacatactg ttataatttt aacagaagat aattatagac
      781 aatatgttga tataccaaaa tgggtggaag aaaagaaaaa caaaggaatc attacgagaa
      841 caaattattc tgatttatta agattatctt tgctagcaaa acatggaggt ttgtggttag
      901 acgccacatt ttattgtaca aaaccaatac cagatgagta ttttgatatc gatatttggt
      961 ctattaggag gcctgattat gctcacgcat ccgtagcatc aggatatttt gcgggttatt
     1021 cattagc
//
LOCUS       DADOZM010000292         1021 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.302, whole genome shotgun sequence.
ACCESSION   DADOZM010000292 DADOZM010000000
VERSION     DADOZM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.302"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..221
                     /locus_tag="J8I47_002943"
     CDS             <1..221
                     /locus_tag="J8I47_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAZ6324317.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..>1021
                     /locus_tag="J8I47_002944"
     CDS             218..>1021
                     /locus_tag="J8I47_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ6324318.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE"
BASE COUNT          336 a          176 c          218 g          291 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attacccatt aatacgtttg cttatactat
      301 taatgacgag tataatttag cgccgaatca aggagactcc agattagcaa ttcctaacaa
      361 aattattttg cacgaaactg gaatagatgc accagcaaga aacgtagccg ccaacatgaa
      421 aaataattat aacggaagca attcttatac tacagatgtt attggtgacg gtgggattgt
      481 ttaccgtgtg ggtgagcaag gatatgtttc gtggggagct ggtaacgcta atccttatgc
      541 gcctgtacag attgaattac agcgcacata tgataaagca ttgtttgaaa aaaactatcg
      601 agcttatatt gaatatacaa gagatagtgc aaaaaaatat ggaattccat tgactcttga
      661 tcaaggaact tctttattta caaaaggaat catttctcat ttgtgggtga caaattatgt
      721 ttgggggaac cacacagatc catatggtta cttatcgcaa atgggagtta gcaaagaaaa
      781 gcttgcttat gatttagctc atggatttac cgatgaaaat ccaacaactt ctgaaaacaa
      841 gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag atggaacaaa
      901 tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg tggctggatg
      961 gcacattgct aactataaat acgagtatat tttcattatg gactacaata ctgggaaaga
     1021 a
//
LOCUS       DADOZM010000293         1010 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.303, whole genome shotgun sequence.
ACCESSION   DADOZM010000293 DADOZM010000000
VERSION     DADOZM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.303"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..>1010
                     /locus_tag="J8I47_002945"
     CDS             138..>1010
                     /locus_tag="J8I47_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ6324319.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          351 a          176 c          185 g          298 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attacgccga ttgtaaaatt
       61 aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccga ccaaagaatt
      121 ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt gtaataatag
      181 agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg aatcgttcaa
      241 gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc ttagaatatt
      301 atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt ttacccgagg
      361 aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat gtgattgttg
      421 gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg
      481 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
      541 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
      601 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
      661 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
      721 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
      781 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
      841 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
      901 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
      961 attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
//
LOCUS       DADOZM010000294          995 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.304, whole genome shotgun sequence.
ACCESSION   DADOZM010000294 DADOZM010000000
VERSION     DADOZM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.304"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(26..913)
                     /locus_tag="J8I47_002946"
     CDS             complement(26..913)
                     /locus_tag="J8I47_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ6324320.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DADOZM010000295          994 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.305, whole genome shotgun sequence.
ACCESSION   DADOZM010000295 DADOZM010000000
VERSION     DADOZM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.305"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..>994)
                     /locus_tag="J8I47_002947"
     rRNA            complement(<1..>994)
                     /locus_tag="J8I47_002947"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DADOZM010000296          986 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.306, whole genome shotgun sequence.
ACCESSION   DADOZM010000296 DADOZM010000000
VERSION     DADOZM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.306"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..704
                     /locus_tag="J8I47_002948"
     CDS             <1..704
                     /locus_tag="J8I47_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6324321.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DADOZM010000297          967 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.307, whole genome shotgun sequence.
ACCESSION   DADOZM010000297 DADOZM010000000
VERSION     DADOZM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.307"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            87..356
                     /locus_tag="J8I47_002949"
     CDS             87..356
                     /locus_tag="J8I47_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ6324322.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="J8I47_002950"
     CDS             349..606
                     /locus_tag="J8I47_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ6324323.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          372 a          104 c          178 g          313 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 gcaataggtt taataatcta ttttaaattt tttgtactag ttttagttaa ttagcaaaaa
      961 caacaaa
//
LOCUS       DADOZM010000298          959 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.308, whole genome shotgun sequence.
ACCESSION   DADOZM010000298 DADOZM010000000
VERSION     DADOZM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.308"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(107..511)
                     /locus_tag="J8I47_002951"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="J8I47_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="J8I47_002952"
     CDS             complement(570..>959)
                     /locus_tag="J8I47_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6324324.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DADOZM010000299          956 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.309, whole genome shotgun sequence.
ACCESSION   DADOZM010000299 DADOZM010000000
VERSION     DADOZM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.309"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            323..956
                     /locus_tag="J8I47_002953"
                     /pseudo
     CDS             323..956
                     /locus_tag="J8I47_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          324 a          180 c          177 g          275 t
ORIGIN      
        1 cagaaagaaa tgaacacctt caattatcta aatctcgaaa atggttagat gtaatcatca
       61 ggaaaaacaa tcttaaacga attactattc atggtcttag acacacacat gctagcttgc
      121 ttttagcaag ctagtgcaaa tattaaagat gtgcaagagc gtttaggcca ctcttctatt
      181 caaatcatta tggatctata tattcatatt acagacaaaa gaaaagaaga aaccgtagcg
      241 caattcgcaa aatataggct ctataattta tgggactgat gaatcaaaat tgattcttca
      301 gtcccattgt tattttaggt atatgattcg tacattgaaa gagttgaaaa actatcttcc
      361 gaagccgtca aaaaggaccc tgccaaaagt attgatggtg gatgaatttc gttcgcacgc
      421 ttcaattgtg gataaaatga gctttatttg tgcggatggc gagactggaa aactcattga
      481 tattttgcct acacgaaaat tgtctcgatt aacacactat tttagtcgct gtgcaaatcc
      541 tgaagcggtg gaatatttag tgacagatat gaacgccgcc tacttccaac tgacaaaacg
      601 tgtcttacca aatgcgaaag tcgtcattga ttgttttcat atcatcaaac atttgaatca
      661 ggcgttcaat gattttcgtg tacaagaaat gaaaagatta caccagttag ggcaacgacg
      721 ggaagccgag aaagtgaaaa agaactggcg ctttcttcta aaaaaccgtc aaaaaatcaa
      781 tcactatgat tacaaaacat ggcgcagttt tcgtgcacct aaatacccgc tactcactga
      841 agcaatgatg attgatcgat tgctggcatt ttcaacgcct ttaaaggacg cctatacttt
      901 tttccatgag ctgaccgaag cctttagaga caaggaatca gacttatttt ttacct
//
LOCUS       DADOZM010000300          943 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.310, whole genome shotgun sequence.
ACCESSION   DADOZM010000300 DADOZM010000000
VERSION     DADOZM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.310"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            2..>943
                     /locus_tag="J8I47_002954"
     rRNA            2..>943
                     /locus_tag="J8I47_002954"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DADOZM010000301          937 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.311, whole genome shotgun sequence.
ACCESSION   DADOZM010000301 DADOZM010000000
VERSION     DADOZM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.311"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..354
                     /locus_tag="J8I47_002955"
     CDS             <1..354
                     /locus_tag="J8I47_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ6324325.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="J8I47_002956"
     CDS             355..903
                     /locus_tag="J8I47_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324326.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          379 a          131 c          198 g          229 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaac
//
LOCUS       DADOZM010000302          931 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.312, whole genome shotgun sequence.
ACCESSION   DADOZM010000302 DADOZM010000000
VERSION     DADOZM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.312"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(156..>931)
                     /gene="menA"
                     /locus_tag="J8I47_002957"
     CDS             complement(156..>931)
                     /gene="menA"
                     /locus_tag="J8I47_002957"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAZ6324327.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          332 a          193 c          161 g          245 t
ORIGIN      
        1 caaaacgctg attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa
       61 taagcccaac caaacgaaaa atataaggag ctatttttat ttggctgttc ttattacttg
      121 atgcagggca gttttttcac aacctcttca tgctgttaaa tggcttgcat aatgattgtt
      181 agtaatagcc ccaatacata actggcattg aataagacca tatttttgat ggaatagctg
      241 aaactgatag gctggggtaa cgtttgcttg aattgatcca aatgcttctt cacaattggc
      301 aaggtcaaga aggtgaccaa gatcggccat tcatagatac cgctcaacaa ccccattaaa
      361 atcacgaaat agcaagcata catcaatagc tggaataaga ctgttcccgc aggacgtccg
      421 atataaaaaa ctagtgtata gcgatgattt tcaatatcac ggtcaagatc ccgcaaatta
      481 ttagccaaca tgatattggc aatcgtaaac accaagggta aggaagctaa tacgattgct
      541 gctatagccc aaagatttcc taccaaatca aattgtccag atgaaaagtt gactgtcaga
      601 tcatagacat ctgaaccgaa tgtattgaca taaacgacca tggcaaaaat tccaaggccc
      661 atagtcaccc cactgaaaat ttcccctaaa ggcattcgag ataaaggaat cggtccgaat
      721 gtatagaata ctccaataaa acaacagata aatcccatca aaagtaaaag ccaacccgta
      781 ttgatcgtta gaataacgcc gataacaaaa acaaatgcaa tcatagctaa aatcatgttt
      841 cgaatcaata caggagatag atttctgcgg ccaatgacat tttcttcata cttatatact
      901 tcagaatgag ccttttcata atccatgtaa t
//
LOCUS       DADOZM010000303          916 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.313, whole genome shotgun sequence.
ACCESSION   DADOZM010000303 DADOZM010000000
VERSION     DADOZM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.313"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..135)
                     /locus_tag="J8I47_002958"
     CDS             complement(<1..135)
                     /locus_tag="J8I47_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAZ6324328.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     D"
     gene            complement(122..451)
                     /locus_tag="J8I47_002959"
     CDS             complement(122..451)
                     /locus_tag="J8I47_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324329.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          269 a          217 c          110 g          320 t
ORIGIN      
        1 gtctaatgct aaaacatcta atcctttaca atttccgcct cttctaaacg tcacaaatcc
       61 tttgtaatcc caacgttgga aagaaacctt aatcgtatgt gttccccatc ggctattcgg
      121 atcataattt ttcatttgtt gtaacctcct cataatcagt tagtactata aaggtatctt
      181 tgtttttatc caaaaaatct aataaatctt ttgtagaaga tatctcttca tcaacttcac
      241 cataataaat accttcgata attgttgcta tatctgaaaa actaattttt actggacatt
      301 tatcgtctac cgcattcaat accgacaaaa ttgcttcttt ttctgcaatc tctcgtttga
      361 atttctttgg agctaatcgc atgacactca agtcattttc aagcttttca atccatgcag
      421 ttttactcat tttcgcaaat ttttgaatca ttcctctacc acctttgcta tctactaaat
      481 tagttttact tcataacgaa tatttttatc gtttttcgtt cactcaaaaa tatataaaat
      541 aaatcgactc gtttatggcg tccgaaacga gaaaccctag tagaaaacgg taaatgctcg
      601 attgatccaa agatcaacgt cttcgcaagt atctacatgt actgcagcac acgacacaat
      661 ttcttgtgcg tcctccccat tacgagaaga cctactcctt attccatgag tttgcgtcaa
      721 agcctagatc ccttagtagc tgctcatgat taatctaggc gctattctaa cttgtctaac
      781 ttctaaattc cctcttgttt actctgtgca tattatttac acaaattcat ttcaccacta
      841 tccaaacaag ggcatttact cgattgacca attttttacg ttaccaatcg taaaaaccta
      901 ccccgtcacc ccccga
//
LOCUS       DADOZM010000304          871 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.314, whole genome shotgun sequence.
ACCESSION   DADOZM010000304 DADOZM010000000
VERSION     DADOZM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.314"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            40..423
                     /locus_tag="J8I47_002960"
     CDS             40..423
                     /locus_tag="J8I47_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324330.1"
                     /translation="MLVSVPYTDQPPLEGIRADGEPLTIADQMFDPKLNQWIILANAL
                     DHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIKQENTALKEKADSLAQINSKMM
                     LASLQNSKDISEIKEQLNPASKGGE"
     gene            425..562
                     /locus_tag="J8I47_002961"
     CDS             425..562
                     /locus_tag="J8I47_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAZ6324331.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            600..>871
                     /locus_tag="J8I47_002962"
     CDS             600..>871
                     /locus_tag="J8I47_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324332.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          318 a          137 c          177 g          239 t
ORIGIN      
        1 cgtactggcg gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac
       61 acagatcagc caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat
      121 caaatgtttg atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac
      181 gatttaaaca atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa
      241 cagatcaatg ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg
      301 aaagaaaaag cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa
      361 aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag
      421 tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga
      481 acaagtaatg gttttcgttc cgttgtgcat tactgaagaa aaagcagata aaatcattag
      541 caaagaagag agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca
      601 tgattaattt aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga
      661 ttttattagt aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa
      721 aagtaagtca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
      781 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat
      841 tggtagaaca cacgcaacaa attaaaactt t
//
LOCUS       DADOZM010000305          846 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.315, whole genome shotgun sequence.
ACCESSION   DADOZM010000305 DADOZM010000000
VERSION     DADOZM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.315"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(226..846)
                     /locus_tag="J8I47_002963"
                     /pseudo
     CDS             complement(226..846)
                     /locus_tag="J8I47_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          213 a          180 c          159 g          294 t
ORIGIN      
        1 aatggctcca tctgcaccaa tagataactg agcactgttc aaagtagaac ggtctatcga
       61 gccaaccagt tgctcaaaac taattggtgg ccgatagcct tctggaattg ttagtatcgt
      121 tgaatcattt ccaccactgc ttttcccatt caacgcaaaa aaataaatag tgacggtttt
      181 tccttcacga taaagctttg ctgatccggt attcccgttt gtaactgtta aggttacgac
      241 ttcatatttg ttgtcgtctg atgtcaatac agattttcct tttgattgaa taccatcttc
      301 gaaatttttc aatccttcga tggattgcgg ttcagtcaaa ctaaccgtat cattcaagcc
      361 tttttcagta tattcaggcg tgatgtccca gctgtagtcg ttcggattgt tgctatcttt
      421 caatccttca ccaaagtatt taaactgact aatattcggg gttcgggtgt cgcctttttc
      481 aattttgagc cagtcaattt ggcatgcgcc ggccgtttct ttaggcaact gaaaaatgcg
      541 aaggtcttta ggcaaacccg gttcaagttt cgttggtgtg aatgttagag accatacgtc
      601 tgtcaatccc tcaactggtt ttagttctcc aaaattaacg ttccaataat tatatgctac
      661 aaaggtttga cttgcgggtt ttgttccttt aagcgtgata gtatacgttt gacctattat
      721 aagctcttct tccgtgttac ctttatatat ttcgtacgcg ctagatttaa ttgggaacgt
      781 cgtaccttta tttgcaatat tctcacccaa cgatgtttta cctacccagt aagggtcatc
      841 tagtaa
//
LOCUS       DADOZM010000306          835 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.316, whole genome shotgun sequence.
ACCESSION   DADOZM010000306 DADOZM010000000
VERSION     DADOZM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.316"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            171..824
                     /locus_tag="J8I47_002964"
     CDS             171..824
                     /locus_tag="J8I47_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ6324333.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          349 a           76 c          153 g          257 t
ORIGIN      
        1 caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct gtttgcattt
       61 attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga tattatagag
      121 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
      181 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      241 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      301 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      361 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      421 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      481 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      541 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      601 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      661 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      721 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      781 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat taaag
//
LOCUS       DADOZM010000307          831 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.318, whole genome shotgun sequence.
ACCESSION   DADOZM010000307 DADOZM010000000
VERSION     DADOZM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.318"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..154
                     /locus_tag="J8I47_002965"
     CDS             <1..154
                     /locus_tag="J8I47_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAZ6324334.1"
                     /translation="ITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRKLERS
                     EDEIEW"
     gene            603..>831
                     /locus_tag="J8I47_002966"
     CDS             603..>831
                     /locus_tag="J8I47_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ6324335.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYL"
BASE COUNT          268 a          135 c          178 g          250 t
ORIGIN      
        1 gatcactcgt ttgtctatcc gttttggtct tgcagaaaag gatatggggc tttctgagga
       61 attagaagtg ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa
      121 attagagcgt tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg
      181 aggcggtttg tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca
      241 tttgctaaaa aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc
      301 gcccttttca aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct
      361 attattgagt tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg
      421 ctggacacgt tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca
      481 ttatacgaat atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag
      541 agttacgtga acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa
      601 atttgagtaa aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca
      661 ggcggttaaa gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa
      721 ttaccatagt cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga
      781 ttttaaattt tgaacagctt atgcgcctaa atgaaggtaa tggttattta a
//
LOCUS       DADOZM010000308          812 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.319, whole genome shotgun sequence.
ACCESSION   DADOZM010000308 DADOZM010000000
VERSION     DADOZM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.319"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..259
                     /locus_tag="J8I47_002967"
     CDS             <1..259
                     /locus_tag="J8I47_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAZ6324336.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            260..>812
                     /locus_tag="J8I47_002968"
     CDS             260..>812
                     /locus_tag="J8I47_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAZ6324337.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNAS"
BASE COUNT          315 a           95 c          144 g          258 t
ORIGIN      
        1 agaaattcat ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt
       61 agatgaatgt atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt
      121 taaagaaatg cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga
      181 aaacgtagta acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa
      241 aatagctgag gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa
      301 ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt
      361 agcaatgata gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa
      421 tacggacagt aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt
      481 agaacgtaaa cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc
      541 attcttacaa ctagaaagag aagataatat atttgattta attatagatt tagatttgac
      601 ttcaccttta cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt
      661 agataaagac gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt
      721 aaaaagaatt gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca
      781 gacacctgaa gtttttgaaa tgaatgcctc aa
//
LOCUS       DADOZM010000309          811 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.320, whole genome shotgun sequence.
ACCESSION   DADOZM010000309 DADOZM010000000
VERSION     DADOZM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.320"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="J8I47_002969"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="J8I47_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAZ6324338.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DADOZM010000310          811 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.321, whole genome shotgun sequence.
ACCESSION   DADOZM010000310 DADOZM010000000
VERSION     DADOZM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.321"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(197..454)
                     /locus_tag="J8I47_002970"
     CDS             complement(197..454)
                     /locus_tag="J8I47_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAZ6324339.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          243 a          141 c          112 g          315 t
ORIGIN      
        1 tataccctct tattttagca tactcagttt tagaaaaagc aaaaaaataa agaaacttat
       61 tcttgataac aagctacgtg acgtatatcc ctaacacaaa aatagccata tcctttggtt
      121 tggatacggc tatttgatat tactcattca attatcaaat cacagtaggt gtgaatctca
      181 tcgggatcgg caatatctac ttctgattag gatcaaaggt gattttctca ataaaggtgt
      241 gatacaaagc tttaacttgc ttcttctcac tctggataag aaagcaatcc aaactagtca
      301 atatctgttg gacatctttg acttgaatcg gttccccttg ttgttgaaag atccctaaga
      361 tttcattctc tcgaatttgt aactgtcttc gtttttcagt caattcattt aaacgatctt
      421 ttaacatcgg aaacaattcg ggactatctt ccaaagcagc ttcctgtttt tgctctaata
      481 gttagatttt gcgtaccatt ttcttctttt attttccttc gattgctgat tttaatatag
      541 tctaaaggaa atttatattt attgtattct ttataacttc aagagaactt cgtttgagta
      601 ttactgtatt tttttaattc cataagaaaa ttagttatta cctgccacct aatcagcaaa
      661 tctgaacctg agtcaatata attaatatta ttttccatct ccaacattgt aagtactgtt
      721 ggtgttaata tagggacttt gtacaattgt atttttttga ttttatcatc tgcacaaagg
      781 agatttttta aatctaatcc ttcttttatt t
//
LOCUS       DADOZM010000311          785 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.322, whole genome shotgun sequence.
ACCESSION   DADOZM010000311 DADOZM010000000
VERSION     DADOZM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.322"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          302 a           74 c          127 g          282 t
ORIGIN      
        1 caagttacaa aaaaatatta taaagaataa tattttctaa aaagtaaact atagtaatgt
       61 ggattatcac cttccaacag tatataaaat gagcgtaaaa acggctattt ttgccttaac
      121 aacgattttt ttgtatacaa atacgatatg ctcattaatt attggaattt gtagtgctgt
      181 ttaaattaat gaaggtgtaa ttatatgagt gatcattttg aaaaagtttg ttgagaagat
      241 gtgcttctag tcatgtgatg ggagaaaata atttaactgt tagagtgaat agttaaatta
      301 ttctggttat atgaaaaaag tagtttctaa aataatcttg cagggattaa cttaaagaaa
      361 ttgagagaat agtttctttg aaatttctat atacaatgag aagctgaaat ttacttttat
      421 ttgatgactg gaaatagaaa ttaaatatat tgtattgtta cattaaagaa aacttgtatg
      481 gaaaaatcag tacaatacca ttatttaatg ttcttcactt aaagcgcaaa aaatatcagt
      541 ttattaaact cgtatataga acaaatatat gtagacgggg cgaaacgatg agtgtaaaaa
      601 aaatgctatt tatggtaagt acaataattg tattaacatc ccgattttgg ggaattaata
      661 ctagtttatc tatgcgatat tttattatgg caatatggga tttatatttt atgttaagct
      721 atttttctta tcgtgagaaa acatataaaa aatacgttat caagaataac ttttggttga
      781 ctata
//
LOCUS       DADOZM010000312          778 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.323, whole genome shotgun sequence.
ACCESSION   DADOZM010000312 DADOZM010000000
VERSION     DADOZM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.323"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>778
                     /locus_tag="J8I47_002971"
     rRNA            <1..>778
                     /locus_tag="J8I47_002971"
                     /product="23S ribosomal RNA"
BASE COUNT          217 a          172 c          231 g          158 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcg
//
LOCUS       DADOZM010000313          761 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.324, whole genome shotgun sequence.
ACCESSION   DADOZM010000313 DADOZM010000000
VERSION     DADOZM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.324"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            51..731
                     /locus_tag="J8I47_002972"
     CDS             51..731
                     /locus_tag="J8I47_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAZ6324340.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          270 a          127 c          152 g          212 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DADOZM010000314          750 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.325, whole genome shotgun sequence.
ACCESSION   DADOZM010000314 DADOZM010000000
VERSION     DADOZM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.325"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(91..>750)
                     /locus_tag="J8I47_002973"
     CDS             complement(91..>750)
                     /locus_tag="J8I47_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAZ6324341.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          175 c          143 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg
//
LOCUS       DADOZM010000315          737 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.326, whole genome shotgun sequence.
ACCESSION   DADOZM010000315 DADOZM010000000
VERSION     DADOZM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.326"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>737
                     /locus_tag="J8I47_002974"
     CDS             <1..>737
                     /locus_tag="J8I47_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ6324342.1"
                     /translation="LKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANNIGIQIRD
                     MTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSE
                     LDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDV
                     SRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKD
                     GKVMEEDTGRTLHLKDAAFIEDPQHNQV"
BASE COUNT          273 a          124 c          166 g          174 t
ORIGIN      
        1 ttaaaacctg aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat
       61 aaaaaaggac agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata
      121 caaattcgag atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacggggg
      181 gcatatgttc gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact
      241 cctacgcagc atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt
      301 tcagaattag ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc
      361 aaacattttg gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag
      421 aatggtcaga cgttggatga taaggaagct gcggaacgtg ggaaaatcat gaatgatgtc
      481 agccgtgtcg caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa
      541 agagaaccag agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg
      601 gcagaacaaa agaatattgt tcctgttggt tcactttgga tcgacaagaa agacgggaaa
      661 gtaatggaag aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat
      721 ccacagcata atcaagt
//
LOCUS       DADOZM010000316          726 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.327, whole genome shotgun sequence.
ACCESSION   DADOZM010000316 DADOZM010000000
VERSION     DADOZM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.327"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..482)
                     /locus_tag="J8I47_002975"
     CDS             complement(<1..482)
                     /locus_tag="J8I47_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ6324343.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     "
BASE COUNT          229 a          134 c          106 g          257 t
ORIGIN      
        1 tccgcaatgt acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag
       61 atgacgtcaa actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat
      121 aatgaatcat tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct
      181 ggaagtatat agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt
      241 atcaacacgt cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct
      301 tcgttagtga agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc
      361 aagtcaatta tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc
      421 gcgcacgtac ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc
      481 acgttcctac ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt
      541 ttagttacta ccaaaaaaaa gatatatatg tactacctta taattaataa gtaaaaggta
      601 gtacataaat aaggaaaagc tgaaatatat tgatttatag ctttcctcaa agtaatgacg
      661 ttatttaatt tatcgagaag cagagaatac tcgcgacttt agtcatgtag acgaatcaga
      721 ttttcg
//
LOCUS       DADOZM010000317          712 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.328, whole genome shotgun sequence.
ACCESSION   DADOZM010000317 DADOZM010000000
VERSION     DADOZM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.328"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..446)
                     /locus_tag="J8I47_002976"
     CDS             complement(<1..446)
                     /locus_tag="J8I47_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ6324344.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DADOZM010000318          696 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.329, whole genome shotgun sequence.
ACCESSION   DADOZM010000318 DADOZM010000000
VERSION     DADOZM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.329"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            261..542
                     /locus_tag="J8I47_002977"
                     /pseudo
     CDS             261..542
                     /locus_tag="J8I47_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            616..>696
                     /locus_tag="J8I47_002978"
     CDS             616..>696
                     /locus_tag="J8I47_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAZ6324345.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLS"
BASE COUNT          253 a           89 c          119 g          235 t
ORIGIN      
        1 aaaagttttt gatcctttat tttcaagtct gatttggcag cttttcggca gctcatttat
       61 ttatggtcag aaatagatgc tgaaatttta atctattagc aaatagtcca gaacgtatga
      121 agaggagtcg gatatcattt gtcattaaca ccataaatat ttataaaatt cgtgttcacc
      181 tacaattaaa tttaatatat aatattcgtt gtaagggctt ataatttttt gttaatgaac
      241 aagataggaa gaaacattta atggacagtt ttcagagatt gtcactagta aatcaatttg
      301 agttattggc taaatttgag gaccaaaaca gatcttatta tgaaagaaaa atcgaaatat
      361 tgagagaagg ttacgagtat cattatgatg atgaaatttg gagtgatctt tctgagccat
      421 ttccgaaaga agattctcga tttgtattag atgtattgaa catgtatcga gatatcaatt
      481 ttagtaaatc aaagctaaac ttagatgaaa accaaattac taattcatac tatactcatt
      541 tcggttctgt tgcaaagttt tccaaaaaat ctattttagt gtaaaattga gaaaaaagac
      601 agagaggaca gagtaatgaa tcattttaaa ggcaaacaat tcaaaaaaga cgtcattatt
      661 gtcgctgttg gttactacct gcgttacaat ctaagc
//
LOCUS       DADOZM010000319          671 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.330, whole genome shotgun sequence.
ACCESSION   DADOZM010000319 DADOZM010000000
VERSION     DADOZM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.330"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          230 a          127 c           88 g          226 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat a
//
LOCUS       DADOZM010000320          635 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.332, whole genome shotgun sequence.
ACCESSION   DADOZM010000320 DADOZM010000000
VERSION     DADOZM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.332"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..270)
                     /locus_tag="J8I47_002979"
     CDS             complement(<1..270)
                     /locus_tag="J8I47_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324346.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="J8I47_002980"
     CDS             complement(276..>635)
                     /locus_tag="J8I47_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAZ6324347.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DADOZM010000321          634 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.333, whole genome shotgun sequence.
ACCESSION   DADOZM010000321 DADOZM010000000
VERSION     DADOZM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.333"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(1..407)
                     /locus_tag="J8I47_002981"
                     /pseudo
     CDS             complement(1..407)
                     /locus_tag="J8I47_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          200 a          117 c          101 g          216 t
ORIGIN      
        1 tcttttcgga ggagtccgtt tgaatgttca tttaatcccc gttgggaagg cgttcctggg
       61 tctgcgaaat aaatatcaat atcattcgta ttactaatac ttttccaatt agagaattct
      121 tttccacaat caaaggtaat tgatttgaaa agatttttgg gtacggattg aagccattca
      181 ttaatcgaat tttcaatatc aactgcctta cggccttctg gtttcaaggc gataatggct
      241 tttgaaagtc gctcaacgag tgtgatgacg gcacttttat ggtggatgcc gacaatcgta
      301 tcaccctcta aatgtccaaa ttcttcttcg aaaacgacat aatctttttt acgctcagaa
      361 atatttcttt tgaatgcctg ttttccacgt ttttccttat aaccatttca ttgtgatcca
      421 cctcttttgt ttattagttt aaaccatcaa accattttta ataaaatatg taaattcctt
      481 tgctcaaaaa agtagcttct ttacgattcg acaatttcaa ataaagcagg taaaaactgg
      541 ctgtcagaaa atagccaaac tgaagcaaaa aataatgtta aaattaatta atgaacgaaa
      601 aaaatccctc acctaatatt acggtgagga attg
//
LOCUS       DADOZM010000322          629 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.334, whole genome shotgun sequence.
ACCESSION   DADOZM010000322 DADOZM010000000
VERSION     DADOZM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.334"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..136)
                     /locus_tag="J8I47_002982"
     CDS             complement(<1..136)
                     /locus_tag="J8I47_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAZ6324348.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     D"
BASE COUNT          177 a          155 c           77 g          220 t
ORIGIN      
        1 cgtctaatgc taaaacatct aatcctttac aatttccgcc tcttctaaac gtcacaaatc
       61 ctttgtaatc ccaacgttgg aaagaaacct taatagtatg tgtgcccaat cgtatattcg
      121 gatcataatt tttcatttgt cattcctcca ttacgtaaat ttcattttct ctatgtttta
      181 tatcaatttt ttcactttat tcaggacttg ttcatatttt atcgtttttc gtttacttaa
      241 aaatacataa aaaatttcga ctcgtttatg gcgtccgaaa cgagaaaccc tagtagacag
      301 cggtaaatgc tcgattgatc caataatcaa cgtcctcgca agtatctaca tgtactgcag
      361 cacacgacac aatttcttgt gcgtcctccc cattacgaga agacctactc cttattccat
      421 gagtttgcgt caaagtctag atcccttagt agctgctcat gattaatcta gacgctattc
      481 taacttgtct aacttctaaa ttccctcttg tttactctgc gcttattatt tacacaaatt
      541 catttcacca ctatccaaac aagcgcattt actcgattga ccaatttttt acgttaccaa
      601 tcgttaaaac ctaccccgtc actccccga
//
LOCUS       DADOZM010000323          614 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.335, whole genome shotgun sequence.
ACCESSION   DADOZM010000323 DADOZM010000000
VERSION     DADOZM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.335"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(157..456)
                     /locus_tag="J8I47_002983"
     CDS             complement(157..456)
                     /locus_tag="J8I47_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAZ6324349.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          200 a          110 c           71 g          233 t
ORIGIN      
        1 tgtttttgtg ttaattaaag gataaaatac aatataaaga aacatctgtt tctttattta
       61 gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac
      121 tatacttttt aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg
      181 tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat
      241 ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg
      301 ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc
      361 tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat catataattt
      421 tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat
      481 ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt
      541 actggattgt taaaaccttt caatacactg ttatggcaac gatttttatt ttaaaaacat
      601 tctaaattaa atca
//
LOCUS       DADOZM010000324          560 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.336, whole genome shotgun sequence.
ACCESSION   DADOZM010000324 DADOZM010000000
VERSION     DADOZM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.336"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>560
                     /locus_tag="J8I47_002984"
     CDS             <1..>560
                     /locus_tag="J8I47_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6324350.1"
                     /translation="FHEDAFRKERINDETCFVFDGTLTYQPEECFHCHYQNKQTIIKW
                     GWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAEDSVSDRRCSIARRVKQAILE
                     LLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQPLPEVVCFDEFKSVKNVSGA
                     MSFVMMDGKTHQLIDIVENRQLNPLR"
BASE COUNT          166 a          122 c          115 g          157 t
ORIGIN      
        1 ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt ttgtttttga
       61 tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa ataagcaaac
      121 gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
      181 gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
      241 agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca aacaagccat
      301 tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga agcacatttc
      361 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt ctttgaacca
      421 gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg tctcgggagc
      481 catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg
      541 gcaactcaat cctttacgcg
//
LOCUS       DADOZM010000325          534 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.337, whole genome shotgun sequence.
ACCESSION   DADOZM010000325 DADOZM010000000
VERSION     DADOZM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.337"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>534
                     /locus_tag="J8I47_002985"
     rRNA            <1..>534
                     /locus_tag="J8I47_002985"
                     /product="23S ribosomal RNA"
BASE COUNT          128 a          116 c          170 g          120 t
ORIGIN      
        1 gtcgagcagg gacgaaagtc gggcttagtg atccggtggt tccgcatgga agggccatcg
       61 ctcaacggat aaaagctacc ctggggataa caggcttatc tcccccaaga gtccacatcg
      121 acggggaggt ttggcacctc gatgtcggct cgtcgcatcc tggggctgta gtcggtccca
      181 agggttgggc tgttcgccca ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac
      241 agttcggtcc ctatccgtcg cgggcgttgg aaatttgaga ggagctgtcc ttagtacgag
      301 aggaccggga tggacttacc gctggtgtac cagttgttct gccaagggca ttgctgggta
      361 gctatgtagg gaagggataa acgctgaaag catctaagtg tgaagcccac ctcaagatga
      421 gatttcccat ttctttaaga aagtaagatc cctgagagat gatcaggtag ataggtcagg
      481 agtggaagta cagtgatgta tggagcggac tgatactaat cgatcgagga ctta
//
LOCUS       DADOZM010000326          526 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.339, whole genome shotgun sequence.
ACCESSION   DADOZM010000326 DADOZM010000000
VERSION     DADOZM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.339"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="J8I47_002986"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="J8I47_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAZ6324351.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DADOZM010000327          525 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.340, whole genome shotgun sequence.
ACCESSION   DADOZM010000327 DADOZM010000000
VERSION     DADOZM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.340"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..342
                     /locus_tag="J8I47_002987"
     CDS             <1..342
                     /locus_tag="J8I47_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAZ6324352.1"
                     /translation="LERMAGRSLIDIKSPIITDMPKAPKHGNKAEDAIIQMMDIEAER
                     DAILAALMALSLISRQILYYSFCDVNKHSNYEIGQLIRGYGEKNVEKLKSIALIEFAE
                     AYKKGVLVQYR"
BASE COUNT          170 a           73 c          137 g          145 t
ORIGIN      
        1 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
       61 ccgaaggcac cgaagcacgg caataaggca gaagacgcga tcattcagat gatggatata
      121 gaagcggaga gagacgcgat tttagcggct ttgatggcgc ttagtctgat tagccgtcag
      181 atactctact acagcttctg tgacgtaaac aagcactcta attatgaaat agggcaattg
      241 atacgaggat acggagagaa gaatgtagag aagctgaaat ccatcgcatt gatcgaattt
      301 gctgaagcat acaaaaaagg cgtgttagtt cagtatcgtt aattttgtag ggtttttgta
      361 gggatagtgt agggtttttg agcggtttaa cgtgatatta tgatagtgtc gaaagattag
      421 tgataggtct aagacaaaat aataataaaa ggaacatcgt tttattattg tttcacaatt
      481 aagcttcgat agacagcagc ggaaatatta agaataagga tgtga
//
LOCUS       DADOZM010000328          514 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.341, whole genome shotgun sequence.
ACCESSION   DADOZM010000328 DADOZM010000000
VERSION     DADOZM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.341"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="J8I47_002988"
     CDS             complement(<1..132)
                     /locus_tag="J8I47_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAZ6324353.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DADOZM010000329          507 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.342, whole genome shotgun sequence.
ACCESSION   DADOZM010000329 DADOZM010000000
VERSION     DADOZM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.342"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            145..348
                     /locus_tag="J8I47_002989"
     CDS             145..348
                     /locus_tag="J8I47_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ6324354.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          206 a           56 c           89 g          156 t
ORIGIN      
        1 acaaaatatt ttacactctc gtcaatcatt gttatataat aaaatgttat ataataaaat
       61 tactaatgat atttaatatt acaagcaatc ttaagaaatt cgaaaaggaa aaaatgatta
      121 tgaaaaagaa agaaaaagaa agtattgaaa cattgtgtta ttctaggaat attaggaact
      181 tgtctagctg gcattggtac aggaatagac gttgacgcag ctacttacta tggaaatggt
      241 ctttattgta acaaagaaaa atgttgggta aattggggac aatcatggtc agaagggtta
      301 aaacgttggg gagataatct ttttggtagt tttagtggag gacgataatg acttcaataa
      361 acattgatca cacaaaaaaa gtcatacatg aattatacaa tagtttaatg acacgaccta
      421 atcagacgaa agcattatta gatattactg atgttttatt tcaggtatat aaaaaaattg
      481 atacagcaaa atatcctgaa attcttg
//
LOCUS       DADOZM010000330          493 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.343, whole genome shotgun sequence.
ACCESSION   DADOZM010000330 DADOZM010000000
VERSION     DADOZM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.343"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="J8I47_002990"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="J8I47_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAZ6324355.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          141 a          102 c           85 g          165 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatctccc atc
//
LOCUS       DADOZM010000331          488 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.344, whole genome shotgun sequence.
ACCESSION   DADOZM010000331 DADOZM010000000
VERSION     DADOZM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.344"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>488
                     /locus_tag="J8I47_002991"
     CDS             <1..>488
                     /locus_tag="J8I47_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAZ6324356.1"
                     /translation="LIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLA
                     STYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFL
                     ISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNY
                     LT"
BASE COUNT          159 a           87 c          106 g          136 t
ORIGIN      
        1 ctgattgcca caatcgttga tattttggga tatgcaattg caccagagta tcttttcaac
       61 gtcttagcgg atcaagcaaa acaagcaacg ttccaattga acgatctaaa caaagccttt
      121 gtgcaattgg catctaccta caatcaattt aatgggttat ttgatgatgt ggatctccaa
      181 tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga cgattaccga agtcatcaag
      241 aaattgaatg atgtagatgt tttagaacac gatggggacg tgatcgggga tgcgtatgag
      301 tttctgatca gtcaatttgc ttcagaagca gggaagaaag ccggagaatt ttacacacct
      361 catatggttt ctgatatgat ggcgcaaatc gtaacgctcg atcaaaagga acgacgattc
      421 tttagtgttt ttgatccaac gatgggttca ggttcattga tgttaaacgt acgtaactac
      481 ttaacaca
//
LOCUS       DADOZM010000332          487 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.345, whole genome shotgun sequence.
ACCESSION   DADOZM010000332 DADOZM010000000
VERSION     DADOZM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.345"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            352..>487
                     /locus_tag="J8I47_002992"
     CDS             352..>487
                     /locus_tag="J8I47_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ6324357.1"
                     /translation="MNDFTTEILKTLANKGDLNGLFRVHLEKAVNTLLKTELTAFLDY
                     E"
BASE COUNT          171 a           88 c           79 g          149 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgttgag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
      301 ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca tatgaacgat
      361 tttactacag aaatcctaaa gactctagcg aacaaaggcg atttgaatgg attattccgt
      421 gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc tttcctcgat
      481 tacgaaa
//
LOCUS       DADOZM010000333          478 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.346, whole genome shotgun sequence.
ACCESSION   DADOZM010000333 DADOZM010000000
VERSION     DADOZM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.346"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>478
                     /locus_tag="J8I47_002993"
     CDS             <1..>478
                     /locus_tag="J8I47_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAZ6324358.1"
                     /translation="IVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYN
                     QFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQF
                     ASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLT"
BASE COUNT          152 a           85 c          105 g          136 t
ORIGIN      
        1 caatcgttga tattttgggg tattcgatct caccagagta tcttttcaac gtcttagcgg
       61 atcaagcaaa acaagcaacg ttccaattga acgatctaaa caaagccttt gtgcaattgg
      121 catctaccta caatcaattt aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac
      181 taggaacaga tgagcaacaa cgaaatgtga cgattaccga agttatcaag aaattgaatg
      241 acgtggaagt tttaggtcac gatggggatg tgatcgggga tgcgtatgaa tttctgatca
      301 gtcaatttgc ttcagaagca gggaagaaag ccggagaatt ttacacgcct catatggttt
      361 ctgatatgat ggcacaaatc gtgacgttgg atcaaaaaga acgacgattc tttagtgtct
      421 ttgacccaac catgggttca ggttccttga tgttaaatgt acgtaactac ttaactca
//
LOCUS       DADOZM010000334          474 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.347, whole genome shotgun sequence.
ACCESSION   DADOZM010000334 DADOZM010000000
VERSION     DADOZM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.347"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(61..258)
                     /locus_tag="J8I47_002994"
     CDS             complement(61..258)
                     /locus_tag="J8I47_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ6324359.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          154 a           88 c           66 g          166 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttcc
//
LOCUS       DADOZM010000335          470 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.348, whole genome shotgun sequence.
ACCESSION   DADOZM010000335 DADOZM010000000
VERSION     DADOZM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.348"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            175..470
                     /locus_tag="J8I47_002995"
                     /pseudo
     CDS             175..470
                     /locus_tag="J8I47_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          184 a           47 c          101 g          138 t
ORIGIN      
        1 ttctggttac gtgattaaaa aatccaaaaa atgagcatag gaacggttat gattttaggg
       61 tgtgttttaa tgagatattg tgatttcatt aacaagtgta tgataatctg ttgttgagtt
      121 agtaaataag taatagagct aactctaaaa taaaactatt aggaggagat gtaggtgaaa
      181 aagtttaaaa agaaaatata tttaggattg gttgttgggg tgagtatgtt tgggttcatg
      241 gctcatacta aacaagtaac tgcagatact aaccaaaaag aaaggattat gagccaatta
      301 gatgactctc aaataagtat gtacccaatg agctaattgt cagtttaaag gctacacaaa
      361 gaatttcaac agagaaaaat aaaccgcaaa atttattggg tgtaaaagtt atggtggaaa
      421 aagatttgac tcttaggaca aatgaaagcg attgtaataa gcgagatcaa
//
LOCUS       DADOZM010000336          468 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.349, whole genome shotgun sequence.
ACCESSION   DADOZM010000336 DADOZM010000000
VERSION     DADOZM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.349"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            1..468
                     /locus_tag="J8I47_002996"
                     /pseudo
     CDS             1..468
                     /locus_tag="J8I47_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a          101 c           89 g          134 t
ORIGIN      
        1 ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga
       61 tggcacattg acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac
      121 gatcattaaa tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
      181 gaccattctg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
      241 agcagaagat tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat
      301 tcttgagcta ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc
      361 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca
      421 gctgttacca gaagtggtgt gtttcgatga attcaaatca gtgaaaaa
//
LOCUS       DADOZM010000337          457 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.351, whole genome shotgun sequence.
ACCESSION   DADOZM010000337 DADOZM010000000
VERSION     DADOZM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.351"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..>457)
                     /locus_tag="J8I47_002997"
     CDS             complement(<1..>457)
                     /locus_tag="J8I47_002997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324360.1"
                     /translation="TLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTH
                     SIKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMN
                     YYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQY"
BASE COUNT          153 a           76 c           58 g          170 t
ORIGIN      
        1 atactgaatt tctgaaaatc gaatttttct cccagttgat agatcaaggg gcagaaaacc
       61 ttttattaca ttgtgagtgt caccatcatt tacaatgtca ttaggaattt tgattttaga
      121 attctgtgga gcacctattg gaagataata attcattata attttgtagt cttcatgagg
      181 aaataaagta cttttaggat tgctaagata ttctcgtacc gaatctaaga atggcttatc
      241 cattgaatct tcatccccat ttaaactaaa aaacattcct acaattgctt ttgcaactct
      301 aagtgaattc attttcttta ttgaatgtgt taaatcagac ttaccattct ttaaacttgt
      361 attgttataa aggaaatttt tattgtcttc tgcaaatcta atatattcct caacatactc
      421 actaccaaaa aaactattac atttactaca taacgta
//
LOCUS       DADOZM010000338          455 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.352, whole genome shotgun sequence.
ACCESSION   DADOZM010000338 DADOZM010000000
VERSION     DADOZM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.352"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>455
                     /locus_tag="J8I47_002998"
     CDS             <1..>455
                     /locus_tag="J8I47_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ6324361.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV"
BASE COUNT          150 a           95 c           90 g          120 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagt
//
LOCUS       DADOZM010000339          452 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.denovo.353, whole genome shotgun sequence.
ACCESSION   DADOZM010000339 DADOZM010000000
VERSION     DADOZM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.denovo.353"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(158..231)
                     /locus_tag="J8I47_002999"
     tRNA            complement(158..231)
                     /locus_tag="J8I47_002999"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Ile,seq:cat)
BASE COUNT          154 a           80 c           84 g          134 t
ORIGIN      
        1 aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa
       61 aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa
      121 aaattctaac acagaacgat tcaaatggct acgttaatgt accctgtagg actcgaacct
      181 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta ctggttgttg
      241 ccacataaag ccataaacaa tcaaccagta gaatgtgtgg caacaaacct gttatcgcat
      301 agcttggagt gtgactatta tgggtgatag tgaagatatg cgataacatc actattttat
      361 cgaatgattt ttatagttgt caatatagtt atgtaatgct cctcaacgag gagctatttt
      421 tatcgtttag gaatatttaa ataccaacgt tt
//
LOCUS       DADOZM010000340        20347 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.039, whole genome shotgun sequence.
ACCESSION   DADOZM010000340 DADOZM010000000
VERSION     DADOZM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.039"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            192..1232
                     /locus_tag="J8I47_003000"
     CDS             192..1232
                     /locus_tag="J8I47_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAZ6324362.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1330..1518
                     /locus_tag="J8I47_003001"
     CDS             1330..1518
                     /locus_tag="J8I47_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6324363.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1576..1929)
                     /locus_tag="J8I47_003002"
     CDS             complement(1576..1929)
                     /locus_tag="J8I47_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ6324364.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2460..2882
                     /locus_tag="J8I47_003003"
     CDS             2460..2882
                     /locus_tag="J8I47_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ6324365.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2879..3109
                     /locus_tag="J8I47_003004"
     CDS             2879..3109
                     /locus_tag="J8I47_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ6324366.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3607..3807
                     /locus_tag="J8I47_003005"
     CDS             3607..3807
                     /locus_tag="J8I47_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ6324367.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3834..5027
                     /locus_tag="J8I47_003006"
     CDS             3834..5027
                     /locus_tag="J8I47_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ6324368.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5095..6660)
                     /gene="guaA"
                     /locus_tag="J8I47_003007"
     CDS             complement(5095..6660)
                     /gene="guaA"
                     /locus_tag="J8I47_003007"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAZ6324369.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6719..7642)
                     /locus_tag="J8I47_003008"
     CDS             complement(6719..7642)
                     /locus_tag="J8I47_003008"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAZ6324370.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7838..8443
                     /locus_tag="J8I47_003009"
     CDS             7838..8443
                     /locus_tag="J8I47_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ6324371.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8751..9767
                     /locus_tag="J8I47_003010"
     CDS             8751..9767
                     /locus_tag="J8I47_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ6324372.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9908..11209
                     /locus_tag="J8I47_003011"
     CDS             9908..11209
                     /locus_tag="J8I47_003011"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAZ6324373.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11255..11917
                     /gene="deoC"
                     /locus_tag="J8I47_003012"
     CDS             11255..11917
                     /gene="deoC"
                     /locus_tag="J8I47_003012"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAZ6324374.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11938..12336
                     /locus_tag="J8I47_003013"
     CDS             11938..12336
                     /locus_tag="J8I47_003013"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAZ6324375.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12402..13484
                     /locus_tag="J8I47_003014"
     CDS             12402..13484
                     /locus_tag="J8I47_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ6324376.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13696..15258
                     /locus_tag="J8I47_003015"
     CDS             13696..15258
                     /locus_tag="J8I47_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ6324377.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15251..16384
                     /locus_tag="J8I47_003016"
     CDS             15251..16384
                     /locus_tag="J8I47_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6324378.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16384..17340
                     /locus_tag="J8I47_003017"
     CDS             16384..17340
                     /locus_tag="J8I47_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ6324379.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17439..18605
                     /gene="deoB"
                     /locus_tag="J8I47_003018"
     CDS             17439..18605
                     /gene="deoB"
                     /locus_tag="J8I47_003018"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAZ6324380.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18629..19447
                     /locus_tag="J8I47_003019"
     CDS             18629..19447
                     /locus_tag="J8I47_003019"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ6324381.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19464..20171
                     /gene="deoD"
                     /locus_tag="J8I47_003020"
     CDS             19464..20171
                     /gene="deoD"
                     /locus_tag="J8I47_003020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ6324382.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6522 a         3643 c         4362 g         5820 t
ORIGIN      
        1 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
       61 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
      121 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
      181 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
      241 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
      301 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
      361 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
      421 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
      481 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
      541 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
      601 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
      661 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
      721 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
      781 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
      841 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
      901 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
      961 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
     1021 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
     1081 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
     1141 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
     1201 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
     1261 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
     1321 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
     1381 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
     1441 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
     1501 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
     1561 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
     1621 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
     1681 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
     1741 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
     1801 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
     1861 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
     1921 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
     1981 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
     2041 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
     2101 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
     2161 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
     2221 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
     2281 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
     2341 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
     2401 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
     2461 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
     2521 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
     2581 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
     2641 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
     2701 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
     2761 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
     2821 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
     2881 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
     2941 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
     3001 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
     3061 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
     3121 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
     3181 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
     3241 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
     3301 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
     3361 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
     3421 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
     3481 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
     3541 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
     3601 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
     3661 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
     3721 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
     3781 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
     3841 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
     3901 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
     3961 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
     4021 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
     4081 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
     4141 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
     4201 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
     4261 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
     4321 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
     4381 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
     4441 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
     4501 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
     4561 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
     4621 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
     4681 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
     4741 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
     4801 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
     4861 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
     4921 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
     4981 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
     5041 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
     5101 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
     5161 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
     5221 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
     5281 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
     5341 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
     5401 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
     5461 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
     5521 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
     5581 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
     5641 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
     5701 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
     5761 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
     5821 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
     5881 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
     5941 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
     6001 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
     6061 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
     6121 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
     6181 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
     6241 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
     6301 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
     6361 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
     6421 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
     6481 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
     6541 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
     6601 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
     6661 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
     6721 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
     6781 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
     6841 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
     6901 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
     6961 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
     7021 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
     7081 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
     7141 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
     7201 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
     7261 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
     7321 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
     7381 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
     7441 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
     7501 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
     7561 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
     7621 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
     7681 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
     7741 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
     7801 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
     7861 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
     7921 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
     7981 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
     8041 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
     8101 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
     8161 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
     8221 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
     8281 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
     8341 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
     8401 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
     8461 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
     8521 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
     8581 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
     8641 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
     8701 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
     8761 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
     8821 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
     8881 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
     8941 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
     9001 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
     9061 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
     9121 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
     9181 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
     9241 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
     9301 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
     9361 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
     9421 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
     9481 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
     9541 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
     9601 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
     9661 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
     9721 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
     9781 tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt
     9841 tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga
     9901 tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa
     9961 agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt
    10021 cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt
    10081 aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa
    10141 ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct
    10201 agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg
    10261 tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga
    10321 atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc
    10381 accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc
    10441 gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt
    10501 ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc
    10561 aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga
    10621 tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga
    10681 aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca
    10741 aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga
    10801 agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg
    10861 agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct
    10921 tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc
    10981 aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt
    11041 gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta
    11101 tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg
    11161 tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc
    11221 ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca
    11281 tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa
    11341 aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca
    11401 attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac
    11461 acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga
    11521 tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat
    11581 cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc
    11641 gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga
    11701 ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt
    11761 gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga
    11821 agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt
    11881 agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg
    11941 gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt
    12001 ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa
    12061 ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt
    12121 ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc
    12181 cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct
    12241 gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca
    12301 ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac
    12361 tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat
    12421 ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg
    12481 gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca
    12541 cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa
    12601 aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt
    12661 caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga
    12721 tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag
    12781 gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa
    12841 ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa
    12901 caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg
    12961 gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga
    13021 atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt
    13081 atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc
    13141 aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc
    13201 gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga
    13261 catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg
    13321 gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa
    13381 cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca
    13441 tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc
    13501 ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc
    13561 caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa
    13621 aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta
    13681 ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa
    13741 gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt
    13801 catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga
    13861 ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt
    13921 ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat
    13981 gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt
    14041 gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc
    14101 aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta
    14161 aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc
    14221 cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg
    14281 atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt
    14341 atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta
    14401 gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct
    14461 caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc
    14521 gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat
    14581 ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga
    14641 catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat
    14701 ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta
    14761 tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg
    14821 aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca
    14881 acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc
    14941 gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg
    15001 gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa
    15061 gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct
    15121 gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa
    15181 ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg
    15241 gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc
    15301 agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat
    15361 ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac
    15421 gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc
    15481 tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg
    15541 ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt
    15601 aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg
    15661 aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt
    15721 tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat
    15781 cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt
    15841 aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa
    15901 aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc
    15961 aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg
    16021 gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc
    16081 gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg
    16141 tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac
    16201 aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt
    16261 cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga
    16321 agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact
    16381 ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca
    16441 ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta
    16501 gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg
    16561 agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt
    16621 atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc
    16681 agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg
    16741 tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga
    16801 ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat
    16861 tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg
    16921 cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat
    16981 gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt
    17041 tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc
    17101 tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg
    17161 ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg
    17221 attccgccag tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt
    17281 ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa
    17341 tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt
    17401 ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc gtacatctga
    17461 tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc ggtgatgttg
    17521 gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca catttagagc
    17581 agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag
    17641 gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca ggccactggg
    17701 aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg
    17761 atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac aaaccttata
    17821 gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc gatttaattg
    17881 tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc attcctttag
    17941 aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat ccgtatatga
    18001 ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca
    18061 atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct ttgaaagaag
    18121 caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga caaggaatca
    18181 ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg aaagttatga
    18241 aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca ttatacggtc
    18301 atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta cgaattccag
    18361 aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat cacggaaacg
    18421 atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg gtttacagta
    18481 aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt tcagcaacga
    18541 ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg aaattattaa
    18601 aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt tacaagaaac
    18661 gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga ttctaggttc
    18721 aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt atgatgagat
    18781 cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt atgggtctct
    18841 ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag gtcattccat
    18901 gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt cagtcattgt
    18961 gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga tgctgattac
    19021 cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg
    19081 ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc
    19141 agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct attctggacc
    19201 aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg atgcagtagg
    19261 gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag tgttaggaat
    19321 ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg aagaagttgt
    19381 agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa cattgacatt
    19441 attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg
    19501 ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg gagacttttt
    19561 tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc ggcaaatata
    19621 aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc accatctatg
    19681 cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt acatgcggtg
    19741 cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca gcgaccagtt
    19801 cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt gatttcaatc
    19861 tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat gtaggaaatg
    19921 ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa ttagctgaac
    19981 ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc gcgaaatacc
    20041 atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt gaagaaacaa
    20101 cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg gaaacagcga
    20161 tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga acatccaagc
    20221 aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg taagcttttg
    20281 aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt
    20341 tcaaaaa
//
LOCUS       DADOZM010000341        13231 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.078, whole genome shotgun sequence.
ACCESSION   DADOZM010000341 DADOZM010000000
VERSION     DADOZM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.078"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            193..690
                     /gene="dfrG"
                     /locus_tag="J8I47_003021"
     CDS             193..690
                     /gene="dfrG"
                     /locus_tag="J8I47_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAZ6324383.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(762..2714)
                     /locus_tag="J8I47_003022"
     CDS             complement(762..2714)
                     /locus_tag="J8I47_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAZ6324384.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2721..3017)
                     /locus_tag="J8I47_003023"
     CDS             complement(2721..3017)
                     /locus_tag="J8I47_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324385.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3192..3995)
                     /locus_tag="J8I47_003024"
     CDS             complement(3192..3995)
                     /locus_tag="J8I47_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ6324386.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(4011..4127)
                     /locus_tag="J8I47_003025"
     CDS             complement(4011..4127)
                     /locus_tag="J8I47_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAZ6324387.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4372..5301)
                     /locus_tag="J8I47_003026"
     CDS             complement(4372..5301)
                     /locus_tag="J8I47_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6324388.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5318..6340)
                     /locus_tag="J8I47_003027"
     CDS             complement(5318..6340)
                     /locus_tag="J8I47_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ6324389.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6337..8364)
                     /locus_tag="J8I47_003028"
     CDS             complement(6337..8364)
                     /locus_tag="J8I47_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324390.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8361..10814)
                     /locus_tag="J8I47_003029"
     CDS             complement(8361..10814)
                     /locus_tag="J8I47_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ6324391.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10798..11193)
                     /locus_tag="J8I47_003030"
     CDS             complement(10798..11193)
                     /locus_tag="J8I47_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ6324392.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(11265..11903)
                     /locus_tag="J8I47_003031"
     CDS             complement(11265..11903)
                     /locus_tag="J8I47_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324393.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(11959..12453)
                     /locus_tag="J8I47_003032"
     CDS             complement(11959..12453)
                     /locus_tag="J8I47_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ6324394.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(12520..>13231)
                     /locus_tag="J8I47_003033"
     CDS             complement(12520..>13231)
                     /locus_tag="J8I47_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAZ6324395.1"
                     /translation="KSSSNYYEGGNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLC
                     TYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQL
                     KISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLY
                     SRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKL
                     IIGISSTVTDYISGYLDK"
BASE COUNT         3938 a         2550 c         2185 g         4558 t
ORIGIN      
        1 ttacatacaa aatgatttat aaaatgcaat tgaaaatgat aattgaatga aaatttgtga
       61 ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac taacgggcag
      121 gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt agttaaaaag
      181 gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag agtgattggc
      241 aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa aaatactaca
      301 aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag agccttacct
      361 gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg ttgtgaaatt
      421 gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat ttttattttc
      481 ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta catcacaaaa
      541 atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga ggaatggaat
      601 gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta ctattttcat
      661 gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa
      721 gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa ataaattata
      781 tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt caaaaccttt
      841 ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca
      901 aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc accacgagct
      961 gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat aaagctattg
     1021 gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc cagagcagat
     1081 aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa tggatgtttg
     1141 atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc ctcttcgtgg
     1201 aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg tagaaacttt
     1261 gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg tccgaattct
     1321 tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg
     1381 ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga gaagtcatca
     1441 tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca atagtgagca
     1501 gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct attagtaatt
     1561 ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga tggttcttgt
     1621 ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag atggtcttct
     1681 ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc ataggcaaca
     1741 tcagaacgga acacatatct tgataagacc tcagcagata caactatata cgtttgcata
     1801 ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg gacgttattt
     1861 aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt tgtttgaagg
     1921 ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca ctccagctta
     1981 tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac tgaaaccctt
     2041 ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg ataagtagtg
     2101 gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca ttgatagcgt
     2161 tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt gaatggggtt
     2221 gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac atcttggatg
     2281 ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga taaaggggtt
     2341 actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta cataccttta
     2401 tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc ttttacatct
     2461 ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca gtaattatat
     2521 tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa cattttgtta
     2581 taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct atcttttaat
     2641 tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt ggctaatctt
     2701 ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt ttcaacaatc
     2761 cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc taaaatgttc
     2821 aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc gtattgctta
     2881 actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt cttatttttt
     2941 ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg taaccatttt
     3001 aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta tatacatttt
     3061 aacttacatt ttgtggtgta aattaacaac aaacgttgat ttaacaataa aaaacgagta
     3121 tcaaattcag ttgcaatttg atactcattt aggtttacat tttcactttt tgtactccaa
     3181 aactgaacta cttaatatgc aagacgttgt cttttttttg tatattcaat tttgaaaaca
     3241 tttccgcaaa gttcagacgg acctcgatgt gttgatgaat aaaatttatt agtaataact
     3301 tctataaggt tatcaatccc tatattactt tttgcacttc catgataaag agggaacaga
     3361 gaacaattct gaaatcttat gctttcctct tgttcgagtt ccaatgcttc taatgattta
     3421 ccggacatat atttctctaa aaggtcatcg tttccctcta ttaccgtatc ccattgttca
     3481 gattcggtaa agttcgtcac acacatatta ggatacagtt ctaccttctg tttgattaca
     3541 atttcggcag aaagtttctc tttaatatcc tgataaaccg ttgataaatc aattccattt
     3601 tggtcaatct tattgataaa aaagattgtg ggaatcccca ttttcctaag tgcatgaaat
     3661 aatatacgag tttgtgcttg tacgccatct tttgcagaaa tcagtagaat tgccccatct
     3721 aaaactgata atgaacgata tacttctgct aagaaatcca tatgtcctgg cgtgtctatg
     3781 atgttcacct tcgtattttc ccactgaaaa gaggttattc ctgtctgaat tgtaattcct
     3841 ctctgacgtt ctaaaagcgt attatccgtc ctcgttgtac ctttgtccac gcttcctaat
     3901 tctgtaatcg ctccactgtt atataataag ctttctgtta aggtagtttt tcctgcatca
     3961 acatgagcta aaactccaat attaataatt ttcatgtgat tttcctccat tcaaaaaccc
     4021 aaaagggcat aaaaatccca gtgataaata cttttatcac tgggattttt atgcataacc
     4081 ataggtatac aaagcataca gatattctct ggatacttta gaatcacatg ataaaggtat
     4141 tcttaaactg ggtacaaaaa actaagccct cctaaaaaag gacatctaat tatttgttcc
     4201 cgctatcaaa ttgacagttt atttaagaat accttgccgc atatttatta actcctttta
     4261 aatagtcact taaataatag cacgtaagag catatttgta aaggaatctc caatttttta
     4321 tcaaaaagag tacatgatta caaagtatct gtaatcatgt accaatattt gttattttac
     4381 aatcttccaa ttatcttgtt tttctaatat caactcaaat tgcgagattt gggttgcctt
     4441 tgtttcttga tctaaatatt tcacagcgac attcacgatt acttggttat cttttctggt
     4501 aaagatagga ttcaccaatt cctcgaaaac atattccttg ttaatcatag gtaacacatg
     4561 attacttaca tagtaagtca attctttttc tgtggcggta ggatagagct ggaagaacgt
     4621 ttctaaaaag ctagtgattt cgtccatcgt ttctgtatct actgtatggt cactttctag
     4681 ctgtttgggt tggtagtccg atttttgagg tttcttactc attgtcggat ttttgataat
     4741 aaccatatta tctgattcat ctatatggac aaccacatga aagctagacg atatagtttc
     4801 cttgtctttg tcttcggtaa taacttgttc aacagaaaac agtacctcaa aggtgttctc
     4861 attcacttga gaaacctgcc acacctgtat atcgttcaca ctagaactag taggaatatc
     4921 cttacgaatc atttcttcat ttaatacctg caattcttcc gtcaaataat gggtcaattt
     4981 ttcatttcgt ttatcaatgg cttcttggga ttgttgccac gaaaagtagt ctttcgcaaa
     5041 ggatttcaca aaactttcta cttggttggt atcgacaatc tgctgtttaa tgatttctgt
     5101 ttcacggaca gtatgagtgt caatggcagt aaaattttta tagattccaa atccgacact
     5161 accaatcaat aaaacccata aaacaaaggt taatttctta tgcgtgccaa ctttaatcat
     5221 cggaactttt ctttgcttgc tcggtttttt agatttcttt ggtttctcct tcttttcttt
     5281 tcgctcgatt tttataatca tttgattcat tcctttctta gttatgaata acacgtctag
     5341 ccccaatcag atggtcttgc caataacgac tgcttaagtc gccataaccg attgggtcgc
     5401 ctgcatgata aaagcgattt ccctctaaat atatcgccac atgagtcaca tatgtgcctg
     5461 cattataggt tgaatgaaag aagataaggt cgcctgcttg ggcttcttcc atagagattt
     5521 cttgcgtagc gtcatactga tcttgggcga cacgagggag agaaatgcca gctttgtcat
     5581 acacccattg tattaagcca ctacaatcaa aagaggtggt aggcgacgcc cctccaaaga
     5641 catagggaaa cccttcgtat ttcaaggctt cgtccataat gacttgcacg gtttcatcat
     5701 caaattctgt tggggaagtg tcggttaggt attgtgaaac caactgaaca taaaactgat
     5761 ttccatagtt atatcgccaa ccaccattca caggtatggc tataggattg ggatagtctg
     5821 ctttttgacc acccgacttc tctttagaaa actgttcggc taattcatag gtatattttt
     5881 tcccatgact cctcacataa tttaaaaagc caccgccata attataagat tggataacag
     5941 aatctatatc cactccttgt tgttctgcac tggttaataa ttcactgaaa tatttaaccc
     6001 cttgtttgat ggactcttcg gtactcaaac tgttgggcgg taaacccaaa gattcggaac
     6061 tttgcataac atcttctgct gtaccgcccg attccacttg cataatggct aagatataag
     6121 aaacatagtc ttcaatccca tattccttac catatttttc aatcaacgga cggtgtgcca
     6181 acacttctgg tgaaacggtc acaccgcctt gtggaataga agaatccttg ttgttttcgc
     6241 cactgtccgt atcatctgca aagaaaatga ctaggaataa taagagaaaa aagaagagag
     6301 agaagaccac actacaaatc actgttattt ttagcttcat gtttgttgtc ctcgcttatc
     6361 cgttctttta tttagcttcc tctgattgaa ttgcttcggt ggtgtctttt gtttttgctc
     6421 ttttttcaat ggtcgctgat gattttcacg tttagtgttt tgtgacaaaa caggcgtgac
     6481 ggtttgctga cgcttgcgaa catgctgctg gttattctga acaggagatg atgggttttc
     6541 ttttcgtctt ttttcttttg ctttatccag ctccaaacgc ttttgagcaa tgttctttcg
     6601 tttttgctcc tgctggttca tacgctgttg tttgcgttga gctttggtat ctgccataga
     6661 ttgtttaaag tctgttacat tatgagtgac attctctttt ccttgatgaa gggcatattt
     6721 gacatgggtt ggtgtcgcct gtatctgttg ttttgcctgc tttcttttat tgactagacg
     6781 atttttcgtg tccaatacat gggctgtctt ttcacctaat cgttgcccga taccgccttg
     6841 ttgctttttc acattggaaa gtgctgtttc tttctttggt gtagtccgtt cattttgacg
     6901 agtatggtta gctttattgg tttgattctg ctttgttgtc attgtctttt gcttcgttga
     6961 ccctgctttt ccacgaaaaa tcttactcat actgcgttga aatcttctgg tttgtcgatt
     7021 catcatttga cgaggtctgc gaaacacttg tctgcccatg ttttgagagt cgttgctttg
     7081 taaagtgaat aggctcatta aatcgcccag tttaaaataa atccctgcaa aggtcacaat
     7141 ttgtaaaaac ataatcatga aaaagggaga acttgctgac agcgaataaa gcatagtgga
     7201 aatactaaag gcaacggtaa taatcaaggt aataccagcc cttgtcataa tcacattaaa
     7261 taacttggta atggcatgcc tgcccatgcc atcaaaactc ggcaacatac ttaataggaa
     7321 actgattggc aaaaacatag cgaaaataat aaatagcact tgcgaaaaaa tcatgattcc
     7381 tgttagaagg aaaacaaaaa tcgagatacc aatattgaat acaaacaaaa agaagactgt
     7441 tcctaatctg gaaatcgtct tcggtagggt taaataggta ttgtcatgat cttcaatttc
     7501 ttcaatcact acattttctc tatcttcacc attgtttgta tctggactgg tagacaataa
     7561 actttccaca cgttcttccc ctaacgcttc tacatcagtg gagccatatt gaagcaatag
     7621 ccatggttgc tttacttgaa cagagaataa gctgtcacgt atcatgtcca cactgtcttt
     7681 cccttgacta tccgtgttag gaagaacaat ttttgtccca atattcagac tggcggtact
     7741 gacatctttg gaaaaatcat tgattttcgc aatataatcg ggtgcatacg caataaagga
     7801 agccgataga ataaacacca cgacaaagtt taaaacagct cgtatcactt tcgtcgtttc
     7861 tcgcttaacc aatcctgtat aggcaacata gattccaaca atgagaataa aaagaaggag
     7921 gaaaccgaca taaaaccctg aactggaaaa gccattcggt gtaatgcctg caatcgtctg
     7981 catgttttcg ccaattgctt gggatgtttg actaatgaaa tctaaggcat aggcttcttt
     8041 caccaaataa cctgtggcgt tcgatagata aagagaaatt gtccaaataa aattagtaat
     8101 ggcatacaat ccatacatca ccgattgtcc tatgccatca ctccaattcc atggaagcca
     8161 gccccagcta ttgtccacat aataatcaag ctgatattga ttcaaaccat acttggagta
     8221 ttcattgctt gtatcaatgg tttcatcaac taagcctgtc gcttgtactg cattgcccag
     8281 cgtgcctaat aaaagtaaaa taacgcccac acaaaggagc gttatccaac tatagtgaag
     8341 aaatttcttt tgtttttgtg tcattcttcc acctcatttc gcacaggcgg tctggtatca
     8401 aaggcatgaa gcaactcttc aaaaatcggg tgaaactgca taacccctac acgaccatag
     8461 atgtctgaaa ctaaacattg tccattttct aattcacgta agcgtttctg attgttttca
     8521 tcttccttgt ccacaccaaa aaattctaag gtctttttga tttccactag gtcagtggaa
     8581 cgaaaggcaa atttcaagcc aatattattc ttcatctttt catctagtaa atcgtccgcg
     8641 ttttgagtaa cgaaatagac ccctgcattc atggcacgtc ctgctcgaac caatcgcata
     8701 gatagagctt ttccttgtgc cacttgtaaa aaactccaag cctcatcaag gtcaacaatt
     8761 ttaaaaatgg aacggtcaga gtggataaag tctaaagcaa aggtactaat gacaatcagc
     8821 attgccacac ttaataattc catggtagtg tattcttcaa aagagctatt ggaatcgggt
     8881 aagaccaaat ctgccacttg aataatattg agttgttttt ccaagctaat ggattgggtg
     8941 acactgccat cactgaataa cagatgggca aaatcataat cgacaaagct ttcgatatgg
     9001 tcagcaatac tattgctgat gggcgtatct tcttttcgca attcatcaat caccaataac
     9061 aaccctcgtt gtttgctttg agttacactc cgaatagctt tacgcaggac aggaaacttc
     9121 tcgctatctc ggctggaaat acctgttaag aacgtgagaa tatcaatggc aaggctttct
     9181 gaatctttcg ggagttttaa aatcacataa gggtcaagta accctttatt gtcgttctca
     9241 ctcgtgagat taacaatatt gatttcatgg gcaatatcag gtaaggtttc tttccattca
     9301 ccacgttcag cttttgggtc aacaatggtt gcctgtccgc caaagagtac tgaataatag
     9361 accagcatat tgttagagaa tgattttccg ccacctaatg aaccaataaa agcggacgct
     9421 aatgcatttg ttacagagcc tttaattcct tgacttgcaa ggctaggttg gagataaata
     9481 tttctgcctg tatcgagatt gtaaccgacg taaataccag aattttcacc aatcatttgt
     9541 gtagcaccga aaccaagacc agccaaaaaa tccgacgtga catattgaat atagtcattg
     9601 atatatcgct tgcttgaggg aataaattca ttatgcaagc ctagcatatc gccaaaaggt
     9661 cgtactaatt tgatagacag atcatcataa aaatctttta cttcatcaca acgttgtttc
     9721 agctcgtcca aattctttgc tgatacccgt accacatagc ttagtttgta catagattct
     9781 ttggtttggt ctaaattact ttctaattca tcgacagatt ctaaagcgtc caccacatta
     9841 gtactggttt cgttgtcgct ttcccatgcg tggctatcta aatctttcag ttctttcttt
     9901 ttattgcgaa cggtagataa ggctttttta ttggcgacaa tttcaacatt catactggta
     9961 tcaacgggga aagtaaactg ctgttgctgg taataaaaaa tttcactatt agggaaatcc
    10021 aattcaccaa caatactatt aattgtaaaa tacgccacat aagtagtggt atcttcctgt
    10081 tcaatccgta aatagcgttg attttcttcg attaaacagc gagtgggttt gagaatatcg
    10141 tatttcttta ccaacacttt tttatcttcg tatcgtttcg gtaaatggta gtcgtagtca
    10201 tcaaaaggga cacctgtttg cccgtaaaga tgttcaataa tataaccaaa atcatctttc
    10261 tccaatttac gaatctgaaa gcgtctggat aatttacttt ctaacaactg ttccatctta
    10321 gaaaagcgtt cgatttccgc cataggcata gaaacaaaat cgcccattaa tttgtgattc
    10381 acaccatgaa agaattccgc aaatgctttt tgaatttctt tcccaacatt ttttacggat
    10441 acttcctgtt cattgaggta gagtttaaaa ccaataaaaa agcgatagtc tacttgattg
    10501 tcaccaatca tagaaattaa cgcttcggtc tgttcatcta ttttttcgta agctatctct
    10561 tttaatcgtc cagtgatttc ttttttcgat tgttcctgtg tggctcgaat acttgattcg
    10621 gttgctagtt gcagagcatg aatcttgcca tcacgatttt gggcaatcaa ttgacagaaa
    10681 ttatcatgaa cttgaatttt ttcatcggaa cttaaaaaac tgtaattata gggaattaat
    10741 tcataataag cgaatacttc gccatcatga ttaaatacga gattatcttc catatattta
    10801 atcggatatg ccatctagtt gttcactcct tacaatcgta atcgattcat tcattgtttc
    10861 tttttttaat cgtatttgtt tgccagcgta ggttacttta gggcgtagag cataggtaat
    10921 gatagacttt agaaatccat agggtttctt tccgtcaaag gtcttttggc acatgaaccc
    10981 agtgagaaca atgggaatcc ctaagtattt gagtagtgct ccgtcaatca gagaaagtgg
    11041 tggtaaactg ccaaagagta tgacgacaaa caaagaaacg ataaaccatg tcatttgcgt
    11101 aaaggtaatt gggaaaggga gttgtaaatc attaatcgca taaatcactt tttctactga
    11161 ccaaatactg gtgtaacttc ttattttttt catgtcttca cgtcctttcc gtaaaaatag
    11221 aaaaagaaca atcaaaatag gtgattgctc tttcaatgct ttaactattc aattttattt
    11281 tttgtccatt ttgcaataat accttcatca gtttcttcat catctggatt cattgaatat
    11341 ctgaaactta aattggtatc tgaccctaat cgttcatata agctgggtct atacaatctc
    11401 gttttattgt cattaaaagg acttcgtttt cccagctcac catttccctt taaaggattt
    11461 cttctaactg aaaaacaata tttttttctg ggatcttctg aatcactttg acttaatgag
    11521 aaacacatgg cttctttttg gtcttcattt ttcttctcac taacttctgt cggaataaat
    11581 cgagcaagag tttggctaaa ttgaaataaa acatctccca agttactact gtattcgata
    11641 ccaaaatatt ctcttattgt tttcgtgtct gtatataatt ttaccgaatt agcatagaca
    11701 gctagcatat ctgaaaaatc gttttccttt aaaaattcta gttttaacag agcatattct
    11761 ggaacttttt cctctttttc aaatagctta acaagtacaa tataattttg ctgtttgtaa
    11821 cgaaagtaaa aggaatctat cacccagcct ttatctttca tgtctagttt taatagttta
    11881 agattttcaa atacatttac cataatttat acctctccgt gctatttatt aatttaatta
    11941 taacacggat tgttaaactt actgacaata ttcaaatacc ccatgagagg taactaggta
    12001 attaccctct atttccatat cacgtcccaa agattggtag tcaatataat tttgtaaatt
    12061 ggaagggact tcaccaagct gtcctgtttc ttccacgtaa taacgggcga catcttccat
    12121 cgaatcacaa tcactataac aatgaatatc ctctttgttt tccagtaatt cttccaagct
    12181 actaaaccac atgccttgaa tttctttgag ttcattataa ataggtgtac cttcgatttc
    12241 ttgaatggct tcacatagac ggttgatttc actaatcggc gtgtattcgt ccacatcaaa
    12301 aggaagctca aaatcatgta ttgcatattc ttcgtagtcc tcatttaaac cgattctttc
    12361 tgccatttct tcctcatcaa ttggtggagt aaaccatgct ccgactaatt caccttcatt
    12421 ataccgtcct aaatttgcca catagacttt catttccaat tcttatcaca tcctttttat
    12481 aaaaatagtc ataaaaaaag tagcaatcag ttaatgacgc tacttatcaa ggtatccgct
    12541 aatataatct gtaactgtac ttgaaatacc tattattagt tttgcttctc tctttccaat
    12601 aatatttgta ttagggtgtg caagtgaatg cctatttctt gcttcattaa gagagctaat
    12661 cattccattt gatgacctta tcgttgtctt cactatatca gcaagttcag taggttgaat
    12721 ttctttttca attaattgtt gtatcctact atataattta gatagatttt cgtctctagg
    12781 aactgtaatt tcatattttt ttagtatttc aattaaatat ccatgaaaag cagtgtgaac
    12841 tctgtcaaag gcacgttgat attcaccatt actcataaaa gactcagcgt cttcaatagc
    12901 ttgtgaaacc acatcactaa ttttcaactg actaaagtta attacttcgt aattctctgg
    12961 aagtggtact aaaatagtat ccactaatac attatcacct tgtttatcat atatggagct
    13021 tgctgtctgg gtaatcaaat ctttatgact ttcaaaaaag tctaaatcat caaaaggaac
    13081 tcttatttta agatatgtgc aaagcgtatt ccagcccttt ttatatcctt tataataagt
    13141 aaaatcgtca gtctcatcta aagttacctc tgatattttc attttagaaa caatctcgtt
    13201 acctccttca taataatttg aagagctctt t
//
LOCUS       DADOZM010000342        13213 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.079, whole genome shotgun sequence.
ACCESSION   DADOZM010000342 DADOZM010000000
VERSION     DADOZM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.079"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(204..2162)
                     /locus_tag="J8I47_003034"
     CDS             complement(204..2162)
                     /locus_tag="J8I47_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ6324396.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="J8I47_003035"
     CDS             complement(2159..3031)
                     /locus_tag="J8I47_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ6324397.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="J8I47_003036"
     CDS             complement(3373..4245)
                     /locus_tag="J8I47_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ6324398.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="J8I47_003037"
     CDS             4373..4942
                     /locus_tag="J8I47_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAZ6324399.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="J8I47_003038"
     CDS             5009..5746
                     /locus_tag="J8I47_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ6324400.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="J8I47_003039"
     CDS             5977..7245
                     /locus_tag="J8I47_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAZ6324401.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="J8I47_003040"
     CDS             7732..8196
                     /locus_tag="J8I47_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ6324402.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="J8I47_003041"
     CDS             8180..8809
                     /locus_tag="J8I47_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ6324403.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr"
                     /locus_tag="J8I47_003042"
     CDS             9163..10641
                     /gene="msr"
                     /locus_tag="J8I47_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ6324404.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="J8I47_003043"
     CDS             10688..11134
                     /locus_tag="J8I47_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAZ6324405.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="J8I47_003044"
     CDS             complement(11333..11848)
                     /locus_tag="J8I47_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ6324406.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="J8I47_003045"
     CDS             11965..13167
                     /locus_tag="J8I47_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ6324407.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DADOZM010000343         8032 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.119, whole genome shotgun sequence.
ACCESSION   DADOZM010000343 DADOZM010000000
VERSION     DADOZM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.119"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            292..567
                     /locus_tag="J8I47_003046"
     CDS             292..567
                     /locus_tag="J8I47_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ6324408.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2652
                     /locus_tag="J8I47_003047"
     CDS             580..2652
                     /locus_tag="J8I47_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAZ6324409.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2817..4703
                     /locus_tag="J8I47_003048"
     CDS             2817..4703
                     /locus_tag="J8I47_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ6324410.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4715..5662
                     /locus_tag="J8I47_003049"
     CDS             4715..5662
                     /locus_tag="J8I47_003049"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAZ6324411.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5682..6209
                     /locus_tag="J8I47_003050"
     CDS             5682..6209
                     /locus_tag="J8I47_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ6324412.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6272..6925)
                     /locus_tag="J8I47_003051"
     CDS             complement(6272..6925)
                     /locus_tag="J8I47_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAZ6324413.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7057..7899
                     /locus_tag="J8I47_003052"
     CDS             7057..7899
                     /locus_tag="J8I47_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ6324414.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         2737 a         1350 c         1695 g         2250 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca aaacatatca
     2701 gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca aataccaaaa
     2761 aaattttcct gaagttcact atttttatga tacaatatcc aagataagga gggaacatgt
     2821 tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat ttgtttgacc
     2881 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
     2941 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
     3001 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
     3061 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
     3121 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
     3181 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
     3241 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
     3301 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
     3361 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
     3421 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
     3481 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
     3541 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
     3601 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
     3661 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
     3721 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
     3781 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
     3841 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     3901 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     3961 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     4021 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     4081 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     4141 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     4201 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     4261 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     4321 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     4381 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     4441 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     4501 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     4561 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     4621 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     4681 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     4741 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     4801 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     4861 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     4921 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     4981 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     5041 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     5101 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     5161 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     5221 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     5281 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     5341 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     5401 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     5461 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     5521 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     5581 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     5641 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     5701 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     5761 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     5821 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     5881 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     5941 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     6001 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     6061 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     6121 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     6181 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     6241 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     6301 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     6361 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     6421 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agccacccca
     6481 taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc aacgtcaacg
     6541 ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa gtccaaccta
     6601 gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc aaaagaaaaa
     6661 tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc aatgaatgaa
     6721 acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca taagagacaa
     6781 tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt cccgcgatac
     6841 tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat atttcatacg
     6901 ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta attaatgaaa
     6961 aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt ttaacatgaa
     7021 taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa aatcgtaaca
     7081 gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg tgtcatgccg
     7141 ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga cggcgaaaga
     7201 tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc accaattgga
     7261 gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat ttcgatccat
     7321 atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa tctaacaaaa
     7381 acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt tcaggtaatt
     7441 cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga agaagtcaaa
     7501 aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa cttggtaaaa
     7561 ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat acgcgtggtg
     7621 atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa gaaaacgttc
     7681 gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt gcgacaaatc
     7741 ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg tttgcaagaa
     7801 ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc tacgcatact
     7861 ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg ataagccctc
     7921 agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa ataattgttt
     7981 tcttttttca taaagactac ctttttgata aaatttttga taacattaga ac
//
LOCUS       DADOZM010000344         5985 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.153, whole genome shotgun sequence.
ACCESSION   DADOZM010000344 DADOZM010000000
VERSION     DADOZM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.153"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(40..1026)
                     /locus_tag="J8I47_003053"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="J8I47_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="J8I47_003054"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="J8I47_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAZ6324415.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="J8I47_003055"
     CDS             1851..3005
                     /locus_tag="J8I47_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAZ6324416.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="J8I47_003056"
     CDS             3220..4188
                     /locus_tag="J8I47_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAZ6324417.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="J8I47_003057"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="J8I47_003057"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAZ6324418.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="J8I47_003058"
     CDS             5218..5826
                     /locus_tag="J8I47_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAZ6324419.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DADOZM010000345         5841 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.156, whole genome shotgun sequence.
ACCESSION   DADOZM010000345 DADOZM010000000
VERSION     DADOZM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.156"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            136..1032
                     /locus_tag="J8I47_003059"
     CDS             136..1032
                     /locus_tag="J8I47_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAZ6324420.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1182..2123)
                     /locus_tag="J8I47_003060"
     CDS             complement(1182..2123)
                     /locus_tag="J8I47_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAZ6324421.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2249..3370
                     /locus_tag="J8I47_003061"
     CDS             2249..3370
                     /locus_tag="J8I47_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ6324422.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3628..5130
                     /locus_tag="J8I47_003062"
     CDS             3628..5130
                     /locus_tag="J8I47_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAZ6324423.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5197..5724
                     /locus_tag="J8I47_003063"
     CDS             5197..5724
                     /locus_tag="J8I47_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ6324424.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1915 a         1031 c         1225 g         1670 t
ORIGIN      
        1 gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata caaatatagg
       61 aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag tgacagagaa
      121 agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa taaatcactt
      181 tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt actacagtcg
      241 gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt ttttttagat
      301 tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc aaaaattgtg
      361 ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca ggcacaagat
      421 gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga acttgggctg
      481 aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga accaaagacg
      541 gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag ctataagcag
      601 gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg agggaatgaa
      661 caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc gcgcgggatt
      721 ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga atccatgtat
      781 tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt ttatcgtcct
      841 gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt tctgcataca
      901 ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa ttgggcaaaa
      961 cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct ctcctctgtt
     1021 gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa caagcacaat
     1081 tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct taaaaaaaga
     1141 gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc gccaaggatt
     1201 ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat
     1261 ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa acgatggttt
     1321 ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta
     1381 gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt tgctgcgata
     1441 gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg cgcgcctagt
     1501 tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt tacacaagct
     1561 tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt tgcttcttga
     1621 aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg
     1681 atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc tgtccacatc
     1741 gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc ttcatgacct
     1801 aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg gatcgaacaa
     1861 aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac gtgttcgttg
     1921 ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc tttaccgtta
     1981 aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca acgatcgatc
     2041 aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc cattgctccg
     2101 gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag
     2161 tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa aaaaagtgtc
     2221 ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg attcatttaa
     2281 aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg aagatcgtaa
     2341 aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta
     2401 ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat tccgtcagcg
     2461 aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca tcgaatctgc
     2521 acaagcattg gggttagatc tacttgataa accaacggat caaactatcc gacttgcttc
     2581 cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag aacaaattat
     2641 tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc aatccttggg
     2701 tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa aacttcattt
     2761 tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag atgttaccaa
     2821 tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaataga
     2881 tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa taaaagattt
     2941 ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta ttggtggagc
     3001 cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc aaatcactgg
     3061 attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc ggatcgatca
     3121 gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa
     3181 accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt ctgattttct
     3241 cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa tggaacacag
     3301 gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt tcaccatcct
     3361 cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat ataggacaaa
     3421 gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac
     3481 cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt tttcgatgta
     3541 tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg aaaataggac
     3601 tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa tctgacattc
     3661 ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt tgacacacaa
     3721 tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact gaatattcta
     3781 caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc ctatttcccg
     3841 caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat tacggatttt
     3901 gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga aatcttatgg
     3961 agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc acttttattt
     4021 gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggt
     4081 agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt ggtcagccat
     4141 gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa aagtcaactg
     4201 gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt
     4261 gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa aacagcagct
     4321 gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca agtcggattc
     4381 atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc tgcacggacg
     4441 atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga gaaagaaaaa
     4501 ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc gtctcaccat
     4561 aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt attcgagcca
     4621 attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa cggtgcagga
     4681 aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat aggagaaaaa
     4741 taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga caatcgagga
     4801 acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt gaacaacctc
     4861 cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat gagtatgggc
     4921 caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact atatacatgg
     4981 gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca actgatcttg
     5041 aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga taaagtatct
     5101 actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca acaaggtatg
     5161 gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac gagacacgga
     5221 gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc gctgactgaa
     5281 aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc tgttccaatc
     5341 acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc agttgctgat
     5401 cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt tggggattat
     5461 gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt cgctgtacga
     5521 ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt attgaaagaa
     5581 tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt gataagagtg
     5641 atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat ggtagataat
     5701 acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa atatttcaag
     5761 gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg ataatataaa
     5821 ctcaaccttt agaaatgaaa t
//
LOCUS       DADOZM010000346         3126 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.206, whole genome shotgun sequence.
ACCESSION   DADOZM010000346 DADOZM010000000
VERSION     DADOZM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.206"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            199..981
                     /locus_tag="J8I47_003064"
     CDS             199..981
                     /locus_tag="J8I47_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAZ6324425.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1107..1838)
                     /gene="erm(A)"
                     /locus_tag="J8I47_003065"
     CDS             complement(1107..1838)
                     /gene="erm(A)"
                     /locus_tag="J8I47_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAZ6324426.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1896..1983)
                     /locus_tag="J8I47_003066"
                     /pseudo
     CDS             complement(1896..1983)
                     /locus_tag="J8I47_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2347..3009
                     /locus_tag="J8I47_003067"
     CDS             2347..3009
                     /locus_tag="J8I47_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ6324427.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1044 a          452 c          577 g         1053 t
ORIGIN      
        1 aaccaaattc aaaaattata tggagatctg tataataaag aataattatt aatctgtaga
       61 caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca gtcatttagg
      121 tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata tgaacataat
      181 cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa tcaagcgatt
      241 caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt atatctattt
      301 ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct agtcgtcgtg
      361 aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat gactatatca
      421 ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat aaataggagt
      481 gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga gtggctcagg
      541 ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt ggctattgtt
      601 ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc aagtatactt
      661 gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc agaactaatt
      721 gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat gtggcaaaca
      781 gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat acctctttta
      841 cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat
      901 gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct
      961 atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta cttttaaaac
     1021 tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa
     1081 attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt gaaaatagaa
     1141 agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt caaagcctgt
     1201 cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt ataaacaaaa
     1261 gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc aagaacaatc
     1321 aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt tttgagcatt
     1381 tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt ttgcaatctt
     1441 ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc aaaggtaatt
     1501 cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt atagtttata
     1561 tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt ctcagagggg
     1621 tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc agtaactgat
     1681 cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat ttctattacg
     1741 ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt tttagaagta
     1801 ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc cttactggtt
     1861 ataatgaact gacattaagc gttcattata accaattaat tttgatttgg ttgataacga
     1921 actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt ccattttttt
     1981 cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat aattgaaacc
     2041 tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac ctttatatgt
     2101 aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt ttgaaacttg
     2161 ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac agtgacttta
     2221 ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag ttttatcccc
     2281 aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta ggaatggggg
     2341 atctttttga atacaataga atcacagttt gataaggttg cagaagatta cgatttcgtg
     2401 aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga
     2461 gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag ttattatgat
     2521 gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa
     2581 ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa tgagaagttt
     2641 gattttattg taagccgaac aacctttcat catttggatg atatagccag cgtaatacag
     2701 caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa tgtatctgaa
     2761 gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt tttaccgcat
     2821 tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac atctaaatca
     2881 tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta cgatttatat
     2941 gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg ggttgtctgg
     3001 acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt tcagagctat
     3061 cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta tgttactatt
     3121 aagtaa
//
LOCUS       DADOZM010000347         1504 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.259, whole genome shotgun sequence.
ACCESSION   DADOZM010000347 DADOZM010000000
VERSION     DADOZM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.259"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            262..373
                     /locus_tag="J8I47_003068"
                     /pseudo
     CDS             262..373
                     /locus_tag="J8I47_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="J8I47_003069"
     CDS             394..477
                     /locus_tag="J8I47_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAZ6324428.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="J8I47_003070"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="J8I47_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAZ6324429.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DADOZM010000348         1351 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.273, whole genome shotgun sequence.
ACCESSION   DADOZM010000348 DADOZM010000000
VERSION     DADOZM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.273"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            48..299
                     /locus_tag="J8I47_003071"
                     /pseudo
     CDS             48..299
                     /locus_tag="J8I47_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            392..1186
                     /locus_tag="J8I47_003072"
                     /note="aph(3')-IIIa"
     CDS             392..1186
                     /locus_tag="J8I47_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAZ6324430.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          323 g          369 t
ORIGIN      
        1 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
       61 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      121 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      181 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      241 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      301 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      361 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      421 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      481 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      541 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      601 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      661 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      721 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      781 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      841 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      901 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
      961 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1021 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1081 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1141 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1201 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1261 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1321 agcgcctacg aggaatttgt atcgataaga a
//
LOCUS       DADOZM010000349         1194 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.285, whole genome shotgun sequence.
ACCESSION   DADOZM010000349 DADOZM010000000
VERSION     DADOZM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.285"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="J8I47_003073"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="J8I47_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAZ6324431.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DADOZM010000350          834 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.317, whole genome shotgun sequence.
ACCESSION   DADOZM010000350 DADOZM010000000
VERSION     DADOZM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.317"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            119..667
                     /gene="aac(6')"
                     /locus_tag="J8I47_003074"
     CDS             119..667
                     /gene="aac(6')"
                     /locus_tag="J8I47_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAZ6324432.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          301 a          126 c          188 g          219 t
ORIGIN      
        1 aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga
       61 gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat
      121 gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact
      181 gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat
      241 gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg
      301 tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc
      361 ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc
      421 cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag
      481 tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga
      541 acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc
      601 aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc
      661 tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta
      721 cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg
      781 gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DADOZM010000351          659 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00013932
            SAMN18083050-rid11850743.guided.331, whole genome shotgun sequence.
ACCESSION   DADOZM010000351 DADOZM010000000
VERSION     DADOZM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18083050
            Sequence Read Archive: SRR13825838
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995491.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995491.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 11:50:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 38 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18083050-rid11850743.guided.331"
                     /strain="AUSMDU00013932"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            9..521
                     /gene="aac(6')-I"
                     /locus_tag="J8I47_003075"
                     /pseudo
     CDS             9..521
                     /gene="aac(6')-I"
                     /locus_tag="J8I47_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
BASE COUNT          270 a           53 c          120 g          216 t
ORIGIN      
        1 aaaatgaaat atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt
       61 taactgatga aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat
      121 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
      181 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      241 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      301 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      361 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      421 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      481 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      541 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      601 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacatt
//
